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936 results on '"Scala, G."'

1. The long-term spectral changes of eta Carinae: are they caused by a dissipating occulter as indicated by CMFGEN models?

Author

Damineli, A., Hillier, D. J., Navarete, F., Moffat, A. F. J., Weigelt, G., Corcoran, M. F., Gull, T. R., Richardson, N. D., Ho, T. P., Madura, T. I., Espinoza-Galeas, D., Hartman, H., Morris, P., Pickett, C. S., Stevens, I. R., Russell, C. M. P., Hamaguchi, K., Jablonski, F. J., Teodoro, M., McGee, P., Cacella, P., Heathcote, B., Harrison, K., Johnston, M., Bohlsen, T., and Di Scala, G.

Subjects
Astrophysics - Solar and Stellar Astrophysics, Astrophysics - Astrophysics of Galaxies
Abstract

Eta Carinae ($\eta$\,Car) exhibits a unique set of P Cygni profiles with both broad and narrow components. Over many decades, the spectrum has changed -- there has been an increase in observed continuum fluxes and a decrease in FeII and HI emission line equivalent widths. The spectrum is evolving towards that of a P Cygni star such as P~Cygni itself and HDE~316285. The spectral evolution has been attributed to intrinsic variations such as a decrease in the mass-loss rate of the primary star or differential evolution in a latitudinal-dependent stellar wind. However intrinsic wind changes conflict with three observational results: the steady long-term bolometric luminosity; the repeating X-ray light curve over the binary period; and the constancy of the dust-scattered spectrum from the Homunculus. We extend previous work that showed a secular strengthening of P~Cygni absorptions by adding more orbital cycles to overcome temporary instabilities and by examining more atomic transitions. {\sc cmfgen} modeling of the primary wind shows that a time-decreasing mass-loss rate is not the best explanation for the observations. However, models with a `small' dissipating absorber in our line-of-site can explain both the increase in brightness and changes in the emission and P Cygni absorption profiles. If the spectral evolution is caused by the dissipating circumstellar medium, and not by intrinsic changes in the binary, the dynamical timescale to recover from the Great Eruption is much less than a century, different from previous suggestions., Comment: 71 pages, 8 figures, 4 long tables, to appear on ApJ

Published
2022

2. Pulsations, eruptions, and evolution of four yellow hypergiants

Author

van Genderen, A. M., Lobel, A., Nieuwenhuijzen, H., Henry, G. W., de Jager, C., Blown, E., Di Scala, G., and van Ballegoij, E. J.

Subjects
Astrophysics - Solar and Stellar Astrophysics
Abstract

We aim to explore the variable photometric and stellar properties of four yellow hypergiants (YHGs), HR8752, HR 5171A, $\rho$ Cas, and HD 179821, and their pulsations of hundreds of days, and long-term variations (LTVs) of years. We tackled multi-colour and visual photometric data sets, looked for photometric indications betraying eruptions or enhanced mass-loss episodes, calculated stellar properties mainly using a published temperature calibration, and investigated the nature of LTVs and their influence on quasi-periods and stellar properties. The $BV$ photometry revealed a high-opacity layer in the atmospheres. When the temperature rises the mass loss increases as well, consequently, as the density of the high-opacity layer. As a result, the absorption in $B$ and $V$ grow. The absorption in $B$, presumably of the order of one to a few 0\fm1, is always higher than in $V$. This difference renders redder and variable $(B-V)$ colour indexes, but the absorption law is unknown. This property of YHGs is unpredictable and explains why spectroscopic temperatures are always higher than photometric ones. We propose shorter distances for $\rho$ Cas and HR 5171A than the accepted ones. Therefore, a correction to decrease the blue luminescence of HR 5171A by polycyclic aromatic hydrocarbon (PAH) molecules is necessary, and HR 5171A would no longer be a member of the cluster Gum48d. HR 5171A is only subject to one source of light variation, not by two as the literature suggests. Eruptive episodes of YHGs prefer relatively cool circumstances when a red evolutionary loop (RL) has shifted the star to the red on the HR diagram. After the eruption, a blue loop evolution (BL) is triggered lasting one to a few decades. The reddening episode of HR 5171A between 1960 and 1974 was most likely due to a red loop evolution, and the reddening after the 1975 eruption was likely due to a shell ejection., Comment: 28 pages, 18 figures, Accepted for Astronomy and Astrophysics

Published
2019
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3. A new bright eclipsing hot subdwarf binary from the ASAS and SuperWASP surveys

Author

Schaffenroth, V., Geier, S., Drechsel, H., Heber, U., Wils, P., Oestensen, R. H., Maxted, P. F. L., and di Scala, G.

Subjects
Astrophysics - Solar and Stellar Astrophysics
Abstract

We report the discovery of a bright (V=11.6 mag) eclipsing hot subdwarf binary of spectral type B with a late main sequence companion from the All Sky Automated Survey (ASAS 102322-3737.0). Such systems are called HW Vir stars after the prototype. The lightcurve shows a grazing eclipse and a strong reflection effect. An orbital period of P=0.13927 d, an inclination of i=65.86{\deg}, a mass ratio q=0.34, a radial velocity semiamplitude K_1=81.0 kms^-1, and other parameters are derived from a combined spectroscopic and photometric analysis. The short period can only be explained by a common envelope origin of the system. The atmospheric parameters (T_eff=28400 K, log g=5.60) are consistent with a core helium-burning star located on the extreme horizontal branch. In agreement with that we derived the most likely sdB mass to be M_sdB=0.46M_sun, close to the canonical mass of such objects. The companion is a late M-dwarf with a mass of M_comp=0.16 M_sun. ASAS 102322-3737.0 is the third brightest of only 12 known HW Virginis systems, which makes it an ideal target for detailed spectroscopic studies and long term photometric monitoring to search for period variations, e.g. caused by a substellar companion.

Published
2013
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6. AB0667 CHARACTERIZING ULTRASOUND-PROVEN ENTHESITIS IN SYSTEMIC LUPUS ERYTHEMATOSUS: PREVALENCE AND IDENTIFICATION OF DISEASE SUBSET WITH LESS FREQUENT RENAL INVOLVEMENT

Author

Fagni, F., primary, Bettiol, A., additional, Silvestri, E., additional, Fedi, R., additional, Palermo, A., additional, Urban, M. L., additional, Mazzotta, R., additional, Malandrino, D., additional, Bello, F., additional, Mattioli, I., additional, Simon, D., additional, Di Scala, G., additional, Schett, G., additional, Prisco, D., additional, and Emmi, G., additional

Published
2023
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9. 'Vecchie' questioni e nuovi strumenti: il crocifisso scolastico e il diritto antidiscriminatorio

Author

Ragone, G, Vanoni, LP, Anrò, I, Croci, F, Ceff, CB, Giannuzzo, L, Scala, G, Cesarini, A, Colombo, F, Losanno, A, Spadaro, I, Ferrari, D, Galluccio, A, Mazzola, R, Pagotto, T, Testa Bappenheim, S, Tira, A, Attollino, S, Ciravegna, M, Geraci, S, Losurdo, R, Marcaccio, G, Negri, A, Suffia, G, Baldassarre, S, Ferrara, F, Ferraro, F, Oliosi, F, Toscano, M, Ragone, G, Vanoni, LP, Anrò, I, Croci, F, Ceff, CB, Giannuzzo, L, Scala, G, Cesarini, A, Colombo, F, Losanno, A, Spadaro, I, Ferrari, D, Galluccio, A, Mazzola, R, Pagotto, T, Testa Bappenheim, S, Tira, A, Attollino, S, Ciravegna, M, Geraci, S, Losurdo, R, Marcaccio, G, Negri, A, Suffia, G, Baldassarre, S, Ferrara, F, Ferraro, F, Oliosi, F, and Toscano, M

Published
2022

11. Mepolizumab for Eosinophilic Granulomatosis With Polyangiitis: A European Multicenter Observational Study

Author

Bettiol, A., Urban, M. L., Dagna, L., Cottin, V., Franceschini, F., Del Giacco, S., Schiavon, F., Neumann, T., Lopalco, G., Novikov, P., Baldini, C., Lombardi, C., Berti, A., Alberici, F., Folci, M., Negrini, S., Sinico, R. A., Quartuccio, L., Lunardi, C., Parronchi, P., Moosig, F., Espigol-Frigole, G., Schroeder, J., Kernder, A. L., Monti, S., Silvagni, E., Crimi, C., Cinetto, F., Fraticelli, P., Roccatello, D., Vacca, A., Mohammad, A. J., Hellmich, B., Samson, M., Bargagli, E., Cohen Tervaert, J. W., Ribi, C., Fiori, D., Bello, F., Fagni, F., Moroni, L., Ramirez, G. A., Nasser, M., Marvisi, C., Toniati, P., Firinu, D., Padoan, R., Egan, A., Seeliger, B., Iannone, F., Salvarani, C., Jayne, D., Prisco, D., Vaglio, A., Emmi, G., Ahmad, K., Beccalli, M., Bonnotte, B., Bortolotti, R., Cariddi, A., Caminati, M., Cid, M. C., Deidda, M., Delvino, P., Scala, G. D., Felicetti, M., Ferro, F., Furini, F., Gelain, E., Ghirelli, G., Holle, J., Losappio, L. M., Mahr, A., Malandrino, D., Marhhold, J., Mattioli, I., Moi, L., Moiseev, S., Muratore, F., Nolasco, S., Olivieri, B., Palermo, A., Regola, F., Sander, O., Scarpa, R., Sciascia, S., Silvestri, E., Susca, N., Terrier, B., Treppo, E., Trezzi, B., Uzzo, M., Vitiello, G., Yacyshyn, E., RS: MHeNs - R3 - Neuroscience, Faculteit FHML Centraal, Bettiol, A, Urban, M, Dagna, L, Cottin, V, Franceschini, F, Del Giacco, S, Schiavon, F, Neumann, T, Lopalco, G, Novikov, P, Baldini, C, Lombardi, C, Berti, A, Alberici, F, Folci, M, Negrini, S, Sinico, R, Quartuccio, L, Lunardi, C, Parronchi, P, Moosig, F, Espígol-Frigolé, G, Schroeder, J, Kernder, A, Monti, S, Silvagni, E, Crimi, C, Cinetto, F, Fraticelli, P, Roccatello, D, Vacca, A, Mohammad, A, Hellmich, B, Samson, M, Bargagli, E, Cohen Tervaert, J, Ribi, C, Fiori, D, Bello, F, Fagni, F, Moroni, L, Ramirez, G, Nasser, M, Marvisi, C, Toniati, P, Firinu, D, Padoan, R, Egan, A, Seeliger, B, Iannone, F, Salvarani, C, Jayne, D, Prisco, D, Vaglio, A, Emmi, G, Jayne, David [0000-0002-1712-0637], and Apollo - University of Cambridge Repository

Subjects
Male, Epidemiology, Birmingham Vasculitis Activity Score, law.invention, Glucocorticoid, Randomized controlled trial, Prednisone, law, Eosinophilic, Monoclonal, Immunology and Allergy, Humanized, PLACEBO, Middle Aged, egpa mepolizumab, Treatment Outcome, SAFETY, Female, ANCA-associated Vasculitis, Biologicals, Eosinophilic Granulomatosis with Polyangiitis (Churg-Strauss), Glucocorticoids, Granulomatosis with polyangiitis, ANCA-associated Vasculiti, medicine.drug, Keywords: ANCA-associated Vasculitis, Adult, medicine.medical_specialty, Immunology, Antibodies, Monoclonal, Humanized, Antibodies, Drug Administration Schedule, Eosinophilia, Granulomatosis with Polyangiitis, Humans, Retrospective Studies, Rheumatology, Internal medicine, medicine, Adverse effect, Asthma, business.industry, medicine.disease, Biological, EGPA, European EGPA Study Group, business, FOLLOW-UP, Mepolizumab
Abstract

OBJECTIVE: Mepolizumab proved to be an efficacious treatment for eosinophilic granulomatosis with polyangiitis (EGPA) at a dose of 300 mg every 4 weeks in the randomized, controlled MIRRA trial. In a few recently reported studies, successful real-life experiences with the approved dose for treating severe eosinophilic asthma (100 mg every 4 weeks) were observed. We undertook this study to assess the effectiveness and safety of mepolizumab 100 mg every 4 weeks and 300 mg every 4 weeks in a large European EGPA cohort. METHODS: We included all patients with EGPA treated with mepolizumab at the recruiting centers in 2015-2020. Treatment response was evaluated from 3 months to 24 months after initiation of mepolizumab. Complete response to treatment was defined as no disease activity (Birmingham Vasculitis Activity Score [BVAS] = 0) and a prednisolone or prednisone dose (or equivalent) of ���4 mg/day. Respiratory outcomes included asthma and ear, nose, and throat (ENT) exacerbations. RESULTS: Two hundred three patients, of whom 191 received a stable dose of mepolizumab (158 received 100 mg every 4 weeks and 33 received 300 mg every 4 weeks) were included. Twenty-five patients (12.3%) had a complete response to treatment at 3 months. Complete response rates increased to 30.4% and 35.7% at 12 months and 24 months, respectively, and rates were comparable between mepolizumab 100 mg every 4 weeks and 300 mg every 4 weeks. Mepolizumab led to a significant reduction in BVAS score, prednisone dose, and eosinophil counts from 3 months to 24 months, with no significant differences observed between 100 mg every 4 weeks and 300 mg every 4 weeks. Eighty-two patients (40.4%) experienced asthma exacerbations (57 of 158 [36%] who received 100 mg every 4 weeks; 17 of 33 [52%] who received 300 mg every 4 weeks), and 31 patients (15.3%) experienced ENT exacerbations. Forty-four patients (21.7%) experienced adverse events (AEs), most of which were nonserious AEs (38 of 44). CONCLUSION: Mepolizumab at both 100 mg every 4 weeks and 300 mg every 4 weeks is effective for the treatment of EGPA. The 2 doses should be compared in the setting of a controlled trial.

Published
2022
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12. CpGmotifs: a tool to discover DNA motifs associated to CpG methylation events

Author

Scala G., Federico A., Greco D., Scala, G., Federico, A., and Greco, D.

Subjects
DNA methylation signature, DNA methylation, Nucleotide Motif, Genome, Human, R-Shiny, Transcription factor, CpG Island, DNA motif, Software, Human
Abstract

Background: The investigation of molecular alterations associated with the conservation and variation of DNA methylation in eukaryotes is gaining interest in the biomedical research community. Among the different determinants of methylation stability, the DNA composition of the CpG surrounding regions has been shown to have a crucial role in the maintenance and establishment of methylation statuses. This aspect has been previously characterized in a quantitative manner by inspecting the nucleotidic composition in the region. Research in this field still lacks a qualitative perspective, linked to the identification of certain sequences (or DNA motifs) related to particular DNA methylation phenomena. Results: Here we present a novel computational strategy based on short DNA motif discovery in order to characterize sequence patterns related to aberrant CpG methylation events. We provide our framework as a user-friendly, shiny-based application, CpGmotifs, to easily retrieve and characterize DNA patterns related to CpG methylation in the human genome. Our tool supports the functional interpretation of deregulated methylation events by predicting transcription factors binding sites (TFBS) encompassing the identified motifs. Conclusions: CpGmotifs is an open source software. Its source code is available on GitHub https://github.com/Greco-Lab/CpGmotifs and a ready-to-use docker image is provided on DockerHub at https://hub.docker.com/r/grecolab/cpgmotifs.

Published
2021

13. Erratum: Management of adult-onset Still's disease with interleukin-1 inhibitors: Evidence- And consensus-based statements by a panel of Italian experts (Arthritis Res Ther (2019) 21:275 DOI: 10.1186/s13075-019-2021-9)

Author

Colafrancesco, S., Manara, M., Bortoluzzi, A., Serban, T., Bianchi, G., Cantarini, L., Ciccia, F., Dagna, L., Govoni, M., Montecucco, C., Priori, R., Ravelli, A., Sfriso, P., Sinigaglia, L., Alivernini, S., Baldissera, E., Bartoloni, E., Berti, A., Bugatti, S., Camellino, D., Cammelli, D., Caporali, R., Caso, F., Cavallaro, E., Cavalli, G., Colaci, M., Costa, L., Di Scala, G., Emmi, G., Frassi, M., Gerli, R., Giacomelli, R., Gremese, E., Iannone, F., Lapadula, G., Leveghi, L., Lopalco, G., Manna, R., Marotto, D., Mathieu, A., Neri, R., Patisso, I., Piga, M., Punzi, L., Romano, M., Ruscitti, P., Salvarani, C., Scarpa, R., Scrivo, R., Talarico, R., Verrecchia, E., Viapiana, O., Vitale, A., AOSD Consensus Group: Alivernini S, Vitiello G., Baldissera, E, Bartoloni, E, Berti, A, Bugatti, S, Camellino, D, Cammelli, D, Caporali, R, Caso, F, Cavallaro, E, Cavalli, G, Colaci, M, Costa, L, di Scala, G, Emmi, G, and Frassi, M

Published
2020

14. Dna methylation levels in mononuclear leukocytes from the mother and her child are associated with ige sensitization to allergens in early life

Author

Acevedo, N., Scala, G., Merid, S.K., Frumento, P., Bruhn, S., Andersson, A., Ogris, C., Bottai, M., Pershagen, G., Koppelman, G.H., Melén, E., Sonnhammer, E., Alm, J., Söderhäll, C., Kere, J., Greco, D., Scheynius, A., STEMM - Stem Cells and Metabolism Research Program, Research Programs Unit, University of Helsinki, Institute of Biotechnology, Acevedo, Nathalie, Scala, Giovanni, Merid, Simon Kebede, Frumento, Paolo, Bruhn, Sören, Andersson, Anna, Ogris, Christoph, Bottai, Matteo, Pershagen, Göran, Koppelman, Gerard H, Melén, Erik, Sonnhammer, Erik, Alm, Johan, Söderhäll, Cilla, Kere, Juha, Greco, Dario, Scheynius, Annika, Groningen Research Institute for Asthma and COPD (GRIAC), Tampere University, and BioMediTech

Subjects
maternal effect, Adult, Male, Maternal effects, Cells, IgE sensitization, Mononuclear, Mothers, ALLADIN, Allergens, Atopy, BAMSE, DNA methylation, Epigenetics, Cells, Cultured, Child, Preschool, Cohort Studies, CpG Islands, Female, Fetal Blood, Genetic Predisposition to Disease, Humans, Hypersensitivity, Immunoglobulin E, Leukocytes, Mononuclear, Pregnancy, DNA Methylation, Article, lcsh:Chemistry, Leukocytes, Alladin, Bamse, Dna Methylation, Ige Sensitization, Maternal Effects, Child, Preschool, lcsh:QH301-705.5, Mother, Cultured, Allergen, lcsh:Biology (General), lcsh:QD1-999, 1182 Biochemistry, cell and molecular biology, 3111 Biomedicine, Cohort Studie, CpG Island, epigenetic, Human
Abstract

DNA methylation changes may predispose becoming IgE-sensitized to allergens. We analyzed whether DNA methylation in peripheral blood mononuclear cells (PBMC) is associated with IgE sensitization at 5 years of age (5Y). DNA methylation was measured in 288 PBMC samples from 74 mother/child pairs from the birth cohort ALADDIN (Assessment of Lifestyle and Allergic Disease During INfancy) using the HumanMethylation450BeadChip (Illumina). PBMCs were obtained from the mothers during pregnancy and from their children in cord blood, at 2 years and 5Y. DNA methylation levels at each time point were compared between children with and without IgE sensitization to allergens at 5Y. For replication, CpG sites associated with IgE sensitization in ALADDIN were evaluated in whole blood DNA of 256 children, 4 years old, from the BAMSE (Swedish abbreviation for Children, Allergy, Milieu, Stockholm, Epidemiology) cohort. We found 34 differentially methylated regions (DMRs) associated with IgE sensitization to airborne allergens and 38 DMRs associated with sensitization to food allergens in children at 5Y (Sidak p ≤ 0.05). Genes associated with airborne sensitization were enriched in the pathway of endocytosis, while genes associated with food sensitization were enriched in focal adhesion, the bacterial invasion of epithelial cells, and leukocyte migration. Furthermore, 25 DMRs in maternal PBMCs were associated with IgE sensitization to airborne allergens in their children at 5Y, which were functionally annotated to the mTOR (mammalian Target of Rapamycin) signaling pathway. This study supports that DNA methylation is associated with IgE sensitization early in life and revealed new candidate genes for atopy. Moreover, our study provides evidence that maternal DNA methylation levels are associated with IgE sensitization in the child supporting early in utero effects on atopy predisposition. publishedVersion

Published
2021

15. Uric acid and late-onset Alzheimer’s disease: results from the ReGAl 2.0 project

Author

Boccardi, Virginia, Carino, Silvia, Marinelli, Elisa, Lapenna, Maria, Caironi, Giulia, Bianco, Anna Rita, Cecchetti, Roberta, Ruggiero, Carmelinda, Mecocci, Patrizia, Defanti, C. A., Dentizzi, C., Gambardella, A., Mari, D., Monacelli, F., Monzani, F., Mossello, E., Onder, G., and Scala, G.

Subjects
Aging, Physiology, Late onset, medicine.disease_cause, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Alzheimer Disease, medicine, 80 and over, Humans, Cognitive Dysfunction, 030212 general & internal medicine, Alzheimer’s, Oxidative stress, Prevention, Uric acid, Aged, Retrospective Studies, Aged, 80 and over, Creatinine, business.industry, Retrospective cohort study, medicine.disease, Uric Acid, chemistry, Italy, Cohort, Female, Geriatrics and Gerontology, Alzheimer's disease, business, Body mass index, 030217 neurology & neurosurgery, Biomarkers
Abstract

It has been suggested that oxidative stress may have a role in the pathogenesis of Alzheimer's disease (AD). Serum uric acid (UA) could exert neuroprotective effects via its antioxidant capacities. Many studies investigated serum UA levels in subjects with AD, but to date, results are conflicting and evidence in old age subjects is weak. In this study, we assess whether serum UA levels would be altered in the AD old age subjects compared to those of initial cognitive impairment and healthy controls. This is a retrospective study with data gathered from the ReGAl 2.0 project (Rete Geriatrica Alzheimer-Geriatric Network on Alzheimer’s disease), a large Italian multicentric clinical-based study. A cohort of 232 subjects, including 65 (healthy controls HC), 95 mild cognitive impairment (MCI), and 72 AD, were included in the study. Serum UA was measured in all subjects by routine laboratory method. The sample population includes 232 subjects, mostly women with a mean age of 79.16 ± 5.64 (range 66–93) years. No significant difference was found in gender distribution between groups. No significant correlation was found in all populations between age and uric acid levels. AD group had significantly lower UA levels as compared with HC. The association of uric acid with AD presence after adjusting for age, gender, body mass index (BMI) and creatinine levels showed that uric acid level was independently associated with the diagnosis of AD. These data indicate that serum UA is reduced in AD, supporting that UA may have a potential protective role against AD in old age.

Published
2021

16. Clinical profile and evolution of patients with juvenile-onset Behçet’s syndrome over a 25-year period: insights from the AIDA network

Author

Sota, J, Rigante, Donato, Lopalco, G, Emmi, G, Gentileschi, S, Gaggiano, C, Ciarcia, L, Berlengiero, V, Mourabi, M, Ricco, N, Barneschi, S, Tosi, Gm, Frediani, B, Tarsia, M, di Scala, G, Vitale, A, Iannone, F, Fabiani, C, Cantarini, L, Rigante D (ORCID:0000-0001-7032-7779), Sota, J, Rigante, Donato, Lopalco, G, Emmi, G, Gentileschi, S, Gaggiano, C, Ciarcia, L, Berlengiero, V, Mourabi, M, Ricco, N, Barneschi, S, Tosi, Gm, Frediani, B, Tarsia, M, di Scala, G, Vitale, A, Iannone, F, Fabiani, C, Cantarini, L, and Rigante D (ORCID:0000-0001-7032-7779)

Abstract

Behçet’s syndrome (BS) represents an understudied topic in pediatrics: the main aims of our study were to characterize demographic and clinical features of a cohort of BS patients with juvenile-onset managed in three tertiary referral centers in Italy, evaluate their evolution in the long-term, and detect any potential differences with BS patients having an adult-onset. Medical records of 64 juvenile-onset and 332 adult-onset BS followed-up over a 2-year period were retrospectively analyzed and compared. Mean age ± SD of first symptom-appearance was 10.92 ± 4.34 years with a female-to-male ratio of 1.06:1. Mucocutaneous signs were the most frequent initial manifestations, followed by uveitis. Throughout the disease course, genital aphthae (76.56%) and pseudofolliculitis (40.63%) prevailed among the mucocutaneous signs, while major organ involvement was represented by gastrointestinal and ocular involvement (43.75 and 34.38%, respectively). No significant differences emerged for both mucocutaneous signs and specific major organ involvement between juvenile-onset and adult BS patients. After excluding nonspecific abdominal pain, juvenile-onset BS patients were less frequently characterized by the development of major organ involvement (p = 0.027). Logistic regression detected the juvenile-onset as a variable associated with reduced risk of long-term major organ involvement (OR 0.495 [0.263–0.932], p = 0.029). In our cohort, juvenile-onset BS resembled the clinical spectrum of adult-onset patients. Pediatric patients with a full-blown disease at onset showed a more frequent mucocutaneous involvement. In addition, patients with juvenile-onset seemed to develop less frequently major organ involvement and had an overall less severe disease course.

Published
2021

18. FunMappOne: A tool to hierarchically organize and visually navigate functional gene annotations in multiple experiments

Author

Scala G., Serra A., Marwah V. S., Saarimaki L. A., Greco D., Scala, G., Serra, A., Marwah, V. S., Saarimaki, L. A., and Greco, D.

Subjects
Databases, Factual, Animal, R-Shiny, Reactome, Pathway visualization, Computational Biology, Functional annotation, Molecular Sequence Annotation, Computer Graphic, Gene, Mice, Gene Ontology, KEGG, Ontology visualization, Software, Human
Abstract

Functional annotation of genes is an essential step in omics data analysis. Multiple databases and methods are currently available to summarize the functions of sets of genes into higher level representations, such as ontologies and molecular pathways. Annotating results from omics experiments into functional categories is essential not only to understand the underlying regulatory dynamics but also to compare multiple experimental conditions at a higher level of abstraction. Several tools are already available to the community to represent and compare functional profiles of omics experiments. However, when the number of experiments and/or enriched functional terms is high, it becomes difficult to interpret the results even when graphically represented. Therefore, there is currently a need for interactive and user-friendly tools to graphically navigate and further summarize annotations in order to facilitate results interpretation also when the dimensionality is high.

Published
2019

19. eUTOPIA: SolUTion for Omics data PreprocessIng and Analysis

Author

Marwah V. S., Scala G., Kinaret P. A. S., Serra A., Alenius H., Fortino V., Greco D., Marwah, V. S., Scala, G., Kinaret, P. A. S., Serra, A., Alenius, H., Fortino, V., and Greco, D.

Subjects
Transcriptomics analysis, R shiny, Gene expression, Microarray
Abstract

Application of microarrays in omics technologies enables quantification of many biomolecules simultaneously. It is widely applied to observe the positive or negative effect on biomolecule activity in perturbed versus the steady state by quantitative comparison. Community resources, such as Bioconductor and CRAN, host tools based on R language that have become standard for high-throughput analytics. However, application of these tools is technically challenging for generic users and require specific computational skills. There is a need for intuitive and easy-to-use platform to process omics data, visualize, and interpret results.

Published
2019

20. Risk of acute arterial and venous thromboembolic events in eosinophilic granulomatosis with polyangiitis (Churg-Strauss syndrome)

Author

Bettiol, A., Sinico, R. A., Schiavon, F., Monti, S., Bozzolo, E. P., Franceschini, F., Govoni, M., Lunardi, C., Guida, G., Lopalco, G., Paolazzi, G., Vacca, A., Gregorini, G., Leccese, P., Piga, M., Conti, F., Fraticelli, P., Quartuccio, L., Alberici, F., Salvarani, C., Bettio, S., Negrini, S., Selmi, C., Sciascia, S., Moroni, G., Colla, L., Manno, C., Urban, M. L., Vannacci, A., Pozzi, M. R., Fabbrini, P., Polti, S., Felicetti, M., Marchi, M. R., Padoan, R., Delvino, P., Caporali, R., Montecucco, C., Dagna, L., Cariddi, A., Toniati, P., Tamanini, S., Furini, F., Bortoluzzi, A., Tinazzi, E., Delfino, L., Badiu, I., Rolla, G., Venerito, V., Iannone, F., Berti, A., Bortolotti, R., Racanelli, V., Jeannin, G., Padula, A., Cauli, A., Priori, R., Gabrielli, A., Bond, M., Tedesco, M., Pazzola, G., Tomietto, P., Pellecchio, M., Marvisi, C., Maritati, F., Palmisano, A., Dejaco, C., Willeit, J., Kiechl, S., Olivotto, I., Willeit, P., Prisco, D., Vaglio, A., Emmi, G., Bargagli, E., Becatti, M., Beccalli, M., Bello, F., Bozzao, F., Canti, V., Cassia, M. A., Cassone, G., Catanoso, M., Chieco-Bianchi, F., Clari, R., Coladonato, L., De Santis, M., Di Scala, G., Fagni, F., Fenaroli, P., Fiorillo, C., Floris, A., Fornaro, M., Galli, E., Generali, E., Giliberti, M., Lascaro, N., Leccese, I., Mattioli, I., Olivieri, B., Osti, N., Peyronel, F., Radin, M., Righetti, G., Salvati, S., Silvestri, E., Susca, N., Tamburini, C., Taurisano, G., Trezzi, B., Trivioli, G., Bettiol, A, Sinico, R, Schiavon, F, Monti, S, Bozzolo, E, Franceschini, F, Govoni, M, Lunardi, C, Guida, G, Lopalco, G, Paolazzi, G, Vacca, A, Gregorini, G, Leccese, P, Piga, M, Conti, F, Fraticelli, P, Quartuccio, L, Alberici, F, Salvarani, C, Bettio, S, Negrini, S, Selmi, C, Sciascia, S, Moroni, G, Colla, L, Manno, C, Urban, M, Vannacci, A, Pozzi, M, Fabbrini, P, Polti, S, Felicetti, M, Marchi, M, Padoan, R, Delvino, P, Caporali, R, Montecucco, C, Dagna, L, Cariddi, A, Toniati, P, Tamanini, S, Furini, F, Bortoluzzi, A, Tinazzi, E, Delfino, L, Badiu, I, Rolla, G, Venerito, V, Iannone, F, Berti, A, Bortolotti, R, Racanelli, V, Jeannin, G, Padula, A, Cauli, A, Priori, R, Gabrielli, A, Bond, M, Tedesco, M, Pazzola, G, Tomietto, P, Pellecchio, M, Marvisi, C, Maritati, F, Palmisano, A, Dejaco, C, Willeit, J, Kiechl, S, Olivotto, I, Willeit, P, Prisco, D, Vaglio, A, and Emmi, G

Subjects
Pulmonary and Respiratory Medicine, Burden of disease, Humans, Churg-Strauss Syndrome, Granulomatosis with Polyangiitis, Venous Thromboembolism, Venous Thrombosis, Churg-strauss syndrome, Criminology, NO, 03 medical and health sciences, 0302 clinical medicine, Medicine, 030212 general & internal medicine, Vascular inflammation, business.industry, Conflict of interest, Cytoplasmic antibody, medicine.disease, 030228 respiratory system, Wegener granulomatosis, arterial and venous thromboembolic events, Eosinophilic Granulomatosis with Polyangiitis (Churg-Strauss syndrome), Organ involvement, business, Production team
Abstract

Eosinophilic Granulomatosis with Polyangiitis (EGPA, Churg-Strauss syndrome) is a rare anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) characterised by respiratory manifestations and systemic organ involvement [1]. Particularly, cardiac manifestations occur in 40–60% of patients, representing the leading cause of mortality [2]. Recent reports suggest that venous thromboembolic events might also represent a consistent burden of disease [3, 4], as already known for the other AAVs [5–7], possibly due to eosinophil-mediated vascular inflammation [5]. Nevertheless, the occurrence of arterial and venous thrombotic events (AVTE) has never been systematically explored in EGPA. Footnotes This manuscript has recently been accepted for publication in the European Respiratory Journal . It is published here in its accepted form prior to copyediting and typesetting by our production team. After these production processes are complete and the authors have approved the resulting proofs, the article will move to the latest issue of the ERJ online. Please open or download the PDF to view this article. Conflict of interest: Alessandra Bettiol Conflict of interest: Renato Alberto Sinico Conflict of interest: Franco Schiavon Conflict of interest: Sara Monti Conflict of interest: Enrica Paola Bozzolo Conflict of interest: Franco Franceschini Conflict of interest: Marcello Govoni Conflict of interest: Claudio Lunardi Conflict of interest: Giuseppe Guida Conflict of interest: Giuseppe Lopalco Conflict of interest: Giuseppe Paolazzi Conflict of interest: Angelo Vacca Conflict of interest: Gina Gregorini Conflict of interest: Pietro Leccese Conflict of interest: Matteo Piga Conflict of interest: Fabrizio Conti Conflict of interest: Paolo Fraticelli Conflict of interest: Luca Quartuccio Conflict of interest: Federico Alberici Conflict of interest: Carlo Salvarani Conflict of interest: Silvano Bettio Conflict of interest: Simone Negrini Conflict of interest: Carlo Selmi Conflict of interest: Savino Sciascia Conflict of interest: Gabriella Moroni Conflict of interest: Loredana Colla Conflict of interest: Carlo Manno Conflict of interest: Maria Letizia Urban Conflict of interest: Alfredo Vannacci Conflict of interest: Maria Rosa Pozzi Conflict of interest: Paolo Fabbrini Conflict of interest: Stefano Polti Conflict of interest: Mara Felicetti Conflict of interest: Maria Rita Marchi Conflict of interest: Roberto Padoan Conflict of interest: Paolo Delvino Conflict of interest: Roberto Caporali Conflict of interest: Carlomaurizio Montecucco Conflict of interest: Lorenzo Dagna Conflict of interest: Adriana Cariddi Conflict of interest: Paola Toniati Conflict of interest: Dr. Tamanini reports other from Glaxo Smith Kline, outside the submitted work. Conflict of interest: Federica Furini Conflict of interest: Alessandra Bortoluzzi Conflict of interest: Elisa Tinazzi Conflict of interest: Lorenzo Delfino Conflict of interest: Iuliana Badiu Conflict of interest: Giovanni Rolla Conflict of interest: Vincenzo Venerito Conflict of interest: Florenzo Iannone Conflict of interest: Alvise Berti Conflict of interest: Roberto Bortolotti Conflict of interest: Vito Racanelli Conflict of interest: Guido Jeannin Conflict of interest: Angela Padula Conflict of interest: Alberto Cauli Conflict of interest: Roberta Priori Conflict of interest: Armando Gabrielli Conflict of interest: Milena Bond Conflict of interest: Martina Tedesco Conflict of interest: Giulia Pazzola Conflict of interest: Paola Tomietto Conflict of interest: Marco Pellecchio Conflict of interest: Chiara Marvisi Conflict of interest: Federica Maritati Conflict of interest: Alessandra Palmisano Conflict of interest: Christian Dejaco Conflict of interest: Johann Willeit Conflict of interest: Stefan Kiechl Conflict of interest: Iacopo Olivotto Conflict of interest: Peter Willeit Conflict of interest: Domenico Prisco Conflict of interest: Augusto Vaglio Conflict of interest: Giacomo Emmi

Published
2020

21. Multi-omics analysis reveals the influence of genetic and environmental risk factors on developing gut microbiota in infants at risk of celiac disease

Author

Leonard, M. M., Karathia, H., Pujolassos, M., Troisi, J., Valitutti, F., Subramanian, P., Camhi, S., Kenyon, V., Colucci, A., Serena, G., Cucchiara, S., Montuori, M., Malamisura, B., Francavilla, R., Elli, L., Fanelli, B., Colwell, R., Hasan, N., Zomorrodi, A. R., Fasano, A., Piemontese, P., Calvi, A., Baldassarre, M., Norsa, L., Trovato, C. M., Raguseo, C. L., Passaro, T., Roggero, P., Crocco, M., Morelli, A., Perrone, M., Chieppa, M., Scala, G., Lionetti, M. E., Catassi, C., Serretiello, A., Vecchi, C., and De Villsante, G. C.

Subjects
Male, Microbiology (medical), Multi-omics analysis, gut microbiome, gut microbiome, Disease, Environment, Biology, Gut flora, Microbiology, lcsh:Microbial ecology, 03 medical and health sciences, 0302 clinical medicine, Immune system, Pregnancy, Risk Factors, Digestive disorder, Metabolome, Genetic predisposition, Bacteroides, Humans, Metabolomics, Celiac disease, Longitudinal Studies, Prospective Studies, 030304 developmental biology, 0303 health sciences, Thioctic Acid, Cesarean Section, Research, Microbiota, Infant, Newborn, Bacteroides dorei, Infant, celiac disease, microbiota, multi-omics analysis, biology.organism_classification, Gastrointestinal Microbiome, Cross-Sectional Studies, Immunology, Environmental Risk Factor, lcsh:QR100-130, Female, 030211 gastroenterology & hepatology, Metagenomics, Methane
Abstract

Background Celiac disease (CD) is an autoimmune digestive disorder that occurs in genetically susceptible individuals in response to ingesting gluten, a protein found in wheat, rye, and barley. Research shows that genetic predisposition and exposure to gluten are necessary but not sufficient to trigger the development of CD. This suggests that exposure to other environmental stimuli early in life, e.g., cesarean section delivery and exposure to antibiotics or formula feeding, may also play a key role in CD pathogenesis through yet unknown mechanisms. Here, we use multi-omics analysis to investigate how genetic and early environmental risk factors alter the development of the gut microbiota in infants at risk of CD. Results Toward this end, we selected 31 infants from a large-scale prospective birth cohort study of infants with a first-degree relative with CD. We then performed rigorous multivariate association, cross-sectional, and longitudinal analyses using metagenomic and metabolomic data collected at birth, 3 months and 6 months of age to explore the impact of genetic predisposition and environmental risk factors on the gut microbiota composition, function, and metabolome prior to the introduction of trigger (gluten). These analyses revealed several microbial species, functional pathways, and metabolites that are associated with each genetic and environmental risk factor or that are differentially abundant between environmentally exposed and non-exposed infants or between time points. Among our significant findings, we found that cesarean section delivery is associated with a decreased abundance of Bacteroides vulgatus and Bacteroides dorei and of folate biosynthesis pathway and with an increased abundance of hydroxyphenylacetic acid, alterations that are implicated in immune system dysfunction and inflammatory conditions. Additionally, longitudinal analysis revealed that, in infants not exposed to any environmental risk factor, the abundances of Bacteroides uniformis and of metabolite 3-3-hydroxyphenylproprionic acid increase over time, while those for lipoic acid and methane metabolism pathways decrease, patterns that are linked to beneficial immunomodulatory and anti-inflammatory effects. Conclusions Overall, our study provides unprecedented insights into major taxonomic and functional shifts in the developing gut microbiota of infants at risk of CD linking genetic and environmental risk factors to detrimental immunomodulatory and inflammatory effects.

Published
2020

22. Detection of Gibberellin-regulated protein (Peamaclein) sensitization among Italian cypress pollen-sensitized patients

Author

Asero R, Abbadessa S, Aruanno A, Barilaro G, Barzaghi C, Bignardi D, Bilò MB, Borro M, Bresciani M, Busa M, Buzzulini F, Cavaliere C, Cecchi L, Ciccarelli A, Cortellini G, Cucinelli F, Deleonardi G, Emiliani F, Farsi A, Ferrarini E, Franchini M, Ingrassia A, Lippolis D, Losappio L, Marra AM, Martini M, Masieri S, Mauro M, Mazzolini M, Muratore L, Murzilli F, Nucera E, Pastorello EA, Pinter E, Polillo BR, Pravettoni V, Quercia O, Rizzi A, Russello M, Sacerdoti C, Scala E, Scala G, Scarpa A, Schroeder J, Uasuf CG, Villalta D, Yang B, Mistrello G, Amato S, and Lidholm J

Subjects
pollen food syndrome, food allergy, cypress pollen allergy, peach, peamaclein, Settore MED/09 - MEDICINA INTERNA, food and beverages
Abstract

Background: Peach gibberellin-regulated protein (peamaclein) has recently emerged as a relevant food allergen in cypress pollen-hypersensitive patients. Objective: We looked for mono-sensitization to peamaclein among Italian cypress-pollen allergic patients. Material and methods: 835 cypress pollen hypersensitive patients from 28 Italian allergy centers under went thorough interview for food-allergic reactions, and SPT with a commercial peach extracts containing peamaclein. In peach reactors, IgE to rPru p 3 was measured, and those scoring negative were enrolled as potentially mono-sensitized to peamaclein. IgE reactivity to rPru p 7 was evaluated by immunoblot and by an experimental ImmunoCAP with rPru p 7. Results: Peach SPT scored positive in 163 (19.5%) patients but 127 (77,9%) were excluded because Pru p 3 reactors. Twenty-four (14,7%, corresponding to 2.8% of the entire study population) were considered as potentially mono-sensitized to peamaclein. Their distribution did not show any geographic preference. Seventeen/24 (70,8%) had a history of food allergy, in most cases (n=15) to peach. Other offending foods included other Rosaceae, citrus fruits, fig, melon, tree nuts, and kiwi. On peach immunoblot, only 3/18 putative peamaclein allergic subjects reacted to a band at about 7kDa; 4 other patients reacted at about 50-60 kDa. Ten/18 (56%) scored positive for Pru p 7 on ImmunoCAP. Conclusion: Peamaclein allergy and sensitization prevalence seem rare in Italy. Most patients react to peach, albeit other Rosaceae fruits and several citrus fruits may also act as offending foods. Peach and cypress pollen probably share also cross-reacting allergens other than peamaclein.

Published
2020

24. Adalimumab effectively controls both anterior and posterior noninfectious uveitis associated with systemic inflammatory diseases: focus on Behçet’s syndrome

Author

Silvestri, E., Bitossi, A., Bettiol, A., Emmi, G., Urban, M. L., Mattioli, I., Di Scala, G., Bacherini, D., Lopalco, G., Venerito, V., Iannone, F., Vitale, A., Tosi, G. M., Rizzo, S., Fabiani, C., Cantarini, L., Virgili, G., Vannozzi, L., and Prisco, D.

Subjects
eye diseases, Adalimumab, Anterior uveitis, Behçet’s syndrome, Macular edema, Ocular relapses, Uveitis
Published
2020

25. Erratum. management of adult-onset still's disease with interleukin-1 inhibitors. evidence- and consensus-based statements by a panel of Italian experts

Author

Colafrancesco, S., Manara, M., Bortoluzzi, A., Serban, T., Bianchi, G., Cantarini, L., Ciccia, F., Dagna, L., Govoni, M., Montecucco, C., Priori, R., Ravelli, A., Sfriso, P., Sinigaglia, L., Alivernini, S., Baldissera, E., Bartoloni, E., Berti, A., Bugatti, S., Camellino, D., Cammelli, D., Caporali, R., Caso, F., Cavallaro, E., Cavalli, G., Colaci, M., Costa, L., Di Scala, G., Emmi, G., Frassi, M., Gerli, R., Giacomelli, R., Gremese, E., Iannone, F., Lapadula, G., Leveghi, L., Lopalco, G., Manna, R., Marotto, D., Mathieu, A., Neri, R., Patisso, I., Piga, M., Punzi, L., Romano, M., Ruscitti, P., Salvarani, C., Scarpa, R., Scrivo, R., Talarico, R., Verrecchia, E., Viapiana, O., Vitale, A., and Vitiello, G.

Subjects
030203 arthritis & rheumatology, 0301 basic medicine, medicine.medical_specialty, Pediatrics, Adult-onset Still's disease, lcsh:Diseases of the musculoskeletal system, business.industry, Published Erratum, MEDLINE, Interleukin, Rheumatology, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Internal medicine, medicine, lcsh:RC925-935, business
Abstract

Following publication of the original article [1], it was brought to our attention that the AOSD Consensus Group was incorrectly tagged and therefore not searchable. The publishers apologize for this error.

Published
2020

26. Detection of Gibberellin-Regulated Protein (Peamaclein) Sensitization among Italian Cypress Pollen-Sensitized Patients

Author

Asero, R, Abbadessa, S, Aruanno, A, Barilaro, G, Barzaghi, C, Bignardi, D, Bilò, M B, Borro, M, Bresciani, M, Busa, M, Buzzulini, F, Cavaliere, C, Cecchi, L, Ciccarelli, A, Cortellini, G, Cucinelli, F, Deleonardi, G, Emiliani, F, Farsi, A, Ferrarini, E, Franchini, M, Ingrassia, A, Lippolis, D, Losappio, L, Marra, A M, Martini, M, Masieri, S, Mauro, M, Mazzolini, M, Muratore, L, Murzilli, F, Nucera, E, Pastorello, E A, Pinter, E, Polillo, B R, Pravettoni, V, Quercia, O, Rizzi, A, Russello, M, Sacerdoti, C, Scala, E, Scala, G, Scarpa, A, Schroeder, J, Uasuf, G G, Villalta, D, Yang, B, Mistrello, G, Amato, S, Lidholm, J, Nucera, E (ORCID:0000-0002-0565-7680), Rizzi, A (ORCID:0000-0002-6795-746X), Asero, R, Abbadessa, S, Aruanno, A, Barilaro, G, Barzaghi, C, Bignardi, D, Bilò, M B, Borro, M, Bresciani, M, Busa, M, Buzzulini, F, Cavaliere, C, Cecchi, L, Ciccarelli, A, Cortellini, G, Cucinelli, F, Deleonardi, G, Emiliani, F, Farsi, A, Ferrarini, E, Franchini, M, Ingrassia, A, Lippolis, D, Losappio, L, Marra, A M, Martini, M, Masieri, S, Mauro, M, Mazzolini, M, Muratore, L, Murzilli, F, Nucera, E, Pastorello, E A, Pinter, E, Polillo, B R, Pravettoni, V, Quercia, O, Rizzi, A, Russello, M, Sacerdoti, C, Scala, E, Scala, G, Scarpa, A, Schroeder, J, Uasuf, G G, Villalta, D, Yang, B, Mistrello, G, Amato, S, Lidholm, J, Nucera, E (ORCID:0000-0002-0565-7680), and Rizzi, A (ORCID:0000-0002-6795-746X)

Abstract

Background: Peach gibberellin-regulated protein (peamaclein) has recently emerged as a relevant food allergen in cypress pollen-hypersensitive patients. Objective: We looked for mono-sensitization to peamaclein among Italian cypress-pollen allergic patients. Material and methods: 835 cypress pollen hypersensitive patients from 28 Italian allergy centers under went thorough interview for food-allergic reactions, and SPT with a commercial peach extracts containing peamaclein. In peach reactors, IgE to rPru p 3 was measured, and those scoring negative were enrolled as potentially mono-sensitized to peamaclein. IgE reactivity to rPru p 7 was evaluated by immunoblot and by an experimental ImmunoCAP with rPru p 7. Results: Peach SPT scored positive in 163 (19.5%) patients but 127 (77,9%) were excluded because Pru p 3 reactors. Twenty-four (14,7%, corresponding to 2.8% of the entire study population) were considered as potentially mono-sensitized to peamaclein. Their distribution did not show any geographic preference. Seventeen/24 (70,8%) had a history of food allergy, in most cases (n=15) to peach. Other offending foods included other Rosaceae, citrus fruits, fig, melon, tree nuts, and kiwi. On peach immunoblot, only 3/18 putative peamaclein allergic subjects reacted to a band at about 7kDa; 4 other patients reacted at about 50-60 kDa. Ten/18 (56%) scored positive for Pru p 7 on ImmunoCAP. Conclusion: Peamaclein allergy and sensitization prevalence seem rare in Italy. Most patients react to peach, albeit other Rosaceae fruits and several citrus fruits may also act as offending foods. Peach and cypress pollen probably share also cross-reacting allergens other than peamaclein.

Published
2020

27. Evaluation of two commercial peach extracts for skin prick testing in the diagnosis of hypersensitivity to lipid transfer protein. A multicenter study

Author

Asero, R, Aruanno, A, Bresciani, Marco Torkel, Brusca, I, Carollo, M, Cecchi, L, Cortellini, G, Deleonardi, G, Farsi, A, Ferrarini, E, Gabrielli, Francesca Augusta, Ingrassia, A, Mauro, M, Murzilli, F, Nucera, Eleonora, Onida, R, Pastorello, E A, Pinter, E, Rizzi, Angela, Russello, M, Sacerdoti, C, Scala, G, Villalta, D, Zampogna, S, Amato, S, Mistrello, G, Scala, E, Bresciani, M, Gabrielli, G, Nucera, E (ORCID:0000-0002-0565-7680), Rizzi, A (ORCID:0000-0002-6795-746X), Asero, R, Aruanno, A, Bresciani, Marco Torkel, Brusca, I, Carollo, M, Cecchi, L, Cortellini, G, Deleonardi, G, Farsi, A, Ferrarini, E, Gabrielli, Francesca Augusta, Ingrassia, A, Mauro, M, Murzilli, F, Nucera, Eleonora, Onida, R, Pastorello, E A, Pinter, E, Rizzi, Angela, Russello, M, Sacerdoti, C, Scala, G, Villalta, D, Zampogna, S, Amato, S, Mistrello, G, Scala, E, Bresciani, M, Gabrielli, G, Nucera, E (ORCID:0000-0002-0565-7680), and Rizzi, A (ORCID:0000-0002-6795-746X)

Abstract

The clinical usefulness of two commercial peach extracts for SPT (by Lofarma SpA and ALK-Abellò, respectively) was compared in a multicenter study carried out in Italy. Peach allergic patients were tested with the two extracts in parallel and underwent the detection of IgE specific for all three peach allergens currently available (Pru p 1, Pru p 3, and Pru p4, respectively). The two extracts were almost identical in terms of sensitivity and specificity, being able to detect virtually all patients sensitized to stable peach allergens (lipid transfer protein [LTP] and, presumably, peamaclein) but scoring negative in patients exclusively sensitive to labile allergens (either PR-10 and/or profilin). Thus, the two extracts represent an excellent tool to carry out a preliminary component-resolved diagnosis of peach allergy at the first patient visit

Published
2020

28. Long-term effectiveness of secukinumab in patients with axial spondyloarthritis

Author

Gentileschi, S, Rigante, Donato, Sota, J, Lopalco, G, Giannotta, Mg, Emmi, G, Di Scala, G, Iannone, F, Fabiani, C, Frediani, B, Cantarini, L, Rigante D (ORCID:0000-0001-7032-7779), Gentileschi, S, Rigante, Donato, Sota, J, Lopalco, G, Giannotta, Mg, Emmi, G, Di Scala, G, Iannone, F, Fabiani, C, Frediani, B, Cantarini, L, and Rigante D (ORCID:0000-0001-7032-7779)

Abstract

Objectives. The primary aim of our study was to evaluate long-term efficacy of secukinumab (SCK) in patients with axial spondyloarthritis (axSpA); secondary aims were to evaluate drug retention rate and to identify differences in the clinical and laboratory assessment according to axSpA clinical features, dosage administered, and biologic treatment lines. Patients and Methods. We collected clinical, demographical, and treatment data from 39 patients affected by axSpA consecutively treated with SCK. Laboratory assessment was based on inflammation parameters; clinical assessment was performed with the Ankylosing Spondylitis Disease Activity Score- (ASDAS-) CRP and Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). Data were recorded at baseline and every 3 months for the first year and then every 6 months in the second year. Results. Twelve males and 27 females were enrolled; both BASDAI and ASDAS-CRP showed a statistically significant reduction during the observation period (p < 0:0001 and p < 0:0001, respectively). C-reactive protein significantly decreased (p = 0:006), with significant reduction at the post hoc analysis between baseline and both 6-month evaluation (p = 0:02) and 24-month visit (p = 0:036). No statistical significance was observed in BASDAI and ASDAS-CRP improvement (p = 0:482 and p = 0:164, respectively) between different dosages administered. No significant differences emerged in the BASDAI and ASDAS-CRP variations between biologic-naïve patients and subjects previously failing to tumour necrosis factor (TNF) inhibitors (p = 0:53 and p = 0:148, respectively). At the end of our observation, 7 out of 39 patients discontinued SCK. The global retention rate at the end of the study period was 78.2%, without any significant differences between biologic-naïve and anti-TNF-failure patients (p = 0:619) or between subjects administered with different SCK dosages (p = 0:614). No adverse events were reported. Conclusions. In our cohort, SCK

Published
2020

37. Evaluation of two commercial peach extracts for skin prick testing in the diagnosis of hypersensitivity to lipid transfer protein. A multicenter study

Author

Asero, R., primary, Aruanno, A., additional, Bresciani, M., additional, Brusca, I., additional, Carollo, M., additional, Cecchi, L., additional, Cortellini, G., additional, Deleonardi, G., additional, Farsi, A., additional, Ferrarini, E., additional, Gabrielli, G., additional, Ingrassia, A., additional, Mauro, M., additional, Murzilli, F., additional, Nucera, E., additional, Onida, R., additional, Pastorello, E.A., additional, Pinter, E., additional, Rizzi, A., additional, Russello, M., additional, Sacerdoti, C., additional, Scala, E., additional, Scala, G., additional, Villalta, D., additional, Zampogna, S., additional, Amato, S., additional, and Mistrello, G., additional

Published
2020
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39. Noninvasive Screening of Fetal Anomalies: The Serum Metabolomic Way

Author

Troisi, J, Sarno, L, Richards, Sm, Symes, Sj, Adair, Dc, Scala, G, Taylor, Rs, Mccowan, Lm, Fasano, A, Martinelli, P, Guida, M., Troisi, J, Sarno, L, Richards, Sm, Symes, Sj, Adair, Dc, Scala, G, Taylor, R, Mccowan, Lm, Fasano, A, Martinelli, P, and Guida, M.

Published
2018

40. Streptococcus thermophilus urease activity boosts Lactobacillus delbrueckii subsp. bulgaricus homolactic fermentation

Author

Arioli, S, Della Scala, G, Remagni, M, Stuknyte, M, Colombo, S, Guglielmetti, S, De Noni, I, Ragg, E, Mora, D, Arioli S., Della Scala G., Remagni M. C., Stuknyte M., Colombo S., Guglielmetti S., De Noni I., Ragg E., Mora D., Arioli, S, Della Scala, G, Remagni, M, Stuknyte, M, Colombo, S, Guglielmetti, S, De Noni, I, Ragg, E, Mora, D, Arioli S., Della Scala G., Remagni M. C., Stuknyte M., Colombo S., Guglielmetti S., De Noni I., Ragg E., and Mora D.

Abstract

The proto-cooperation between Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus in the yogurt consortium enhances the growth rate and size of each population. In contrast, the independent growth of the two species in milk leads to a slower growth rate and a smaller population size. In this study, we report the first evidence that the urease activity of S. thermophilus increases the intracellular pH of L. delbrueckii in the absence of carbon source. However, in milk, in the presence of lactose the alkalizing effect of urea-derived ammonia was not detectable. Nevertheless, based on glucose consumption and lactic acid production at different pHin, L. delbrueckii showed an optimum of glycolysis and homolactic fermentation at alkaline pH values. In milk, we observed that ammonia provided by urea hydrolysis boosted lactic acid production in S. thermophilus and in L. delbrueckii when the species were grown alone or in combination. Therefore, we propose that urease activity acts as an altruistic cooperative trait, which is costly for urease-positive individuals but provides a local benefit because other individuals can take advantage of urease-dependent ammonia release.

Published
2017

46. Real-world effectiveness of apremilast in multirefractory mucosal involvement of Behçet’s disease

Author

Lopalco, G, Venerito, V, Leccese, P, Emmi, G, Cantarini, L, Lascaro, N, Di Scala, G, Fabiani, C, Rigante, Donato, Iannone, F, Rigante D (ORCID:0000-0001-7032-7779), Lopalco, G, Venerito, V, Leccese, P, Emmi, G, Cantarini, L, Lascaro, N, Di Scala, G, Fabiani, C, Rigante, Donato, Iannone, F, and Rigante D (ORCID:0000-0001-7032-7779)

Abstract

Relapsing oral and genital ulcers (OGUs) represent the stigmata of Behçet’s disease (BD) and may be very painful, affecting both quality of life and relationships. A wide number of topical and immunosuppressive drugs can be used to treat ulcers [1], but failures are commonly reported. The efficacy of the phosphodiesterase-4 inhibitor apremilast has been proven in OGUs of BD in two randomized clinical trials (RCT) [2, 3], whereas only two case reports are available until now [4, 5]. We aimed at evaluating the real-world effectiveness of apremilast in BD patients with OGUs refractory to conventional and/or biologic treatments. We retrospectively evaluated patients classified as BD, according to International Criteria for BD [6] and International Study Group [7] criteria, who underwent apremilast (30 mg twice daily) for multirefractory OGUs from November 2017 to January 2019. The number of OGUs was assessed at baseline and either at 3 and 6 months. Pain from ulcers and BD activity were evaluated via 100-mm visual-analogue scale (VAS) and BD Current Activity Form (BDCAF). We also recorded the number of oral and genital ulcer flares both in the 4 weeks prior to apremilast start and throughout the observation period (Table 1 and Supplementary Table 2). The occurrence of adverse events was also reported. Paired t-test or Wilcoxon matched-pair signed rank test were used for statistical analysis. The off-label use of apremilast was approved by the Hospital Ethics Committee in compliance with the Declaration of Helsinki. All patients provided a written informed consent. Thirteen patients (females 9/13) with disease duration (mean ± SD) of 154 ± 167 months were analysed (Table 1). At 3 months, (data from 12/13 patients) active OGUs were significantly less (p=0.02 for both) than baseline (Table 2). Three patients stopped the treatment due to diarrhoea. At 6 months, active oral ulcers and oral relapses were still lower than baseline (p=0.03 for both), whereas only a positive tr

Published
2019

48. Identification of C12orf4 as a gene for autosomal recessive intellectual disability

Author

Anju K Philips, Pinelli M, Ci, Bie, Mustonen A, Määttä T, Hh, Arts, Wu K, Roepman R, Js, Moilanen, Raza S, Varilo T, Scala G, Cocozza S, Gilissen C, Kl, Gassen, Järvelä I, Philips, A. K., Pinelli, M., de Bie, C. I., Mustonen, A., Maatta, T., Arts, H. H., Wu, K., Roepman, R., Moilanen, J. S., Raza, S., Varilo, T., Scala, G., Cocozza, S., Gilissen, C., van Gassen, K. L. I., and Jarvela, I.

Subjects
Male, Genotype, Intellectual disability, Missense variant, Genes, Recessive, C12orf4, Case Reports, whole exome sequencing, Consanguinity, Netherland, Frameshift variant, Intellectual Disability, Genetics, Journal Article, Humans, Exome, Genetic Predisposition to Disease, Genetics(clinical), Amino Acid Sequence, Child, Finland, Netherlands, Aged, Family Health, Base Sequence, Geography, Sequence Homology, Amino Acid, Intracellular Signaling Peptides and Proteins, Whole exome sequencing, Sequence Analysis, DNA, Founder effect, missense variant, frameshift variant, Founder Effect, Pedigree, Renal disorders Radboud Institute for Molecular Life Sciences [Radboudumc 11], Intracellular Signaling Peptides and Protein, Mutation, Female, Human
Abstract

Contains fulltext : 169700.pdf (Publisher’s version ) (Closed access) Intellectual disability (ID) is a major health problem in our society. Genetic causes of ID remain unknown because of its vast heterogeneity. Here we report two Finnish families and one Dutch family with affected individuals presenting with mild to moderate ID, neuropsychiatric symptoms and delayed speech development. By utilizing whole exome sequencing (WES), we identified a founder missense variant c.983T>C (p.Leu328Pro) in seven affected individuals from two Finnish consanguineous families and a deletion c.799_1034-429delinsTTATGA (p.Gln267fs) in one affected individual from a consanguineous Dutch family in the C12orf4 gene on chromosome 12. Both the variants co-segregated in the respective families as an autosomal recessive trait. Screening of the p.Leu328Pro variant showed enrichment in the North Eastern sub-isolate of Finland among anonymous local blood donors with a carrier frequency of 1:53, similar to other disease mutations with a founder effect in that region. To date, only one Arab family with a three affected individuals with a frameshift insertion variant in C12orf4 has been reported. In summary, we expand and establish the clinical and mutational spectrum of C12orf4 variants. Our findings implicate C12orf4 as a causative gene for autosomal recessive ID.

Published
2017

50. Clinical determination of carbohydrate-deficient transferrin (CDT) in alcohol addicts: A random sample of the general population in Naples, Italy

Author

Reccia, L. A, Morelli, R. A, Boccella, S. B, Iovine, R. A, Zaia, D. C, Crinisio, A. D, Leone, A. D, Marabese, I. B, Scala, G. E, Cante, D. F, Bemail Author, V., Stella, L., MARABESE, Ida, GUIDA, Francesca, DE NOVELLIS, Vito, Reccia, L. A, Morelli, R. A, Boccella, S. B, Iovine, R. A, Zaia, D. C, Crinisio, A. D, Leone, A. D, Marabese, I. B, Scala, G. E, Guida, Francesca, Cante, D. F, DE NOVELLIS, Vito, Bemail Author, V., Stella, L., and Marabese, Ida

Published
2017

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