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594 results on '"Sayers, A. R."'

2. High SARS-CoV-2 incidence and asymptomatic fraction during Delta and Omicron BA.1 waves in The Gambia

Author

Jarju, Sheikh, Wenlock, Rhys D., Danso, Madikoi, Jobe, Dawda, Jagne, Ya Jankey, Darboe, Alansana, Kumado, Michelle, Jallow, Yusupha, Touray, Mamlie, Ceesay, Ebrima A., Gaye, Hoja, Gaye, Biran, Tunkara, Abdoulie, Kandeh, Sheriff, Gomes, Marie, Sylva, Ellen Lena, Toure, Fatoumata, Hornsby, Hailey, Lindsey, Benjamin B., Nicklin, Martin J., Sayers, Jon R., Sesay, Abdul K., Kucharski, Adam, Hodgson, David, Kampmann, Beate, and de Silva, Thushan I.

Published
2024
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3. Interfaith?: A Critical Examination of the Interfaith Learning and Development (ILDT) Framework for Religious, Secular, and Spiritual Identities

Author

Nielsen, J. Cody, Edwards, Sachi, and Sayers, Matthew R.

Abstract

In a recent volume of the "Journal of College and Character," Matt Mayhew and Alyssa Rockenbach presented their frameworks by which they have designed and utilized their IDEALS study over the past several years. In this present article, the authors contend that despite their rigorous research, the researchers and measurements oftentimes have lacked recognition and acknowledgement of Christian biases and hegemony and thus have limited their results. We propose several remediations for how the data can be helpful and effective and suggest future scholarship which evaluates more critical approaches to the subject of religious, secular, and spiritual identities (RSSIs).

Published
2022
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4. Establishing SARS-CoV-2 membrane protein-specific antibodies as a valuable serological target via high-content microscopy

Author

Williams, Daniel M., Hornsby, Hailey R., Shehata, Ola M., Brown, Rebecca, Gallis, Marta, Meardon, Naomi, Newman, Thomas A.H., Plowright, Megan, Zafred, Domen, Shun-Shion, Amber S.M., Hodder, Anthony J., Bliss, Deepa, Metcalfe, Andrew, Edgar, James R., Gordon, David E., Sayers, Jon R., Nicklin, Martin J., Carroll, Miles, Collini, Paul J., Brown, Stephen, de Silva, Thushan I., and Peden, Andrew A.

Published
2023
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11. Efficient overexpression and purification of severe acute respiratory syndrome coronavirus 2 nucleocapsid proteins in Escherichia coli.

Author

Brudenell, Emma L., Pohare, Manoj B., Zafred, Domen, Phipps, Janine, Hornsby, Hailey R., Darby, John F., Junxiao Dai, Liggett, Ellen, Cain, Kathleen M., Barran, Perdita E., de Silva, Thushan I., and Sayers, Jon R.

Subjects
SARS-CoV-2, CORONAVIRUSES, ESCHERICHIA coli, SYNTHETIC genes
Abstract

The fundamental biology of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein (Ncap), its use in diagnostic assays and its potential application as a vaccine component have received considerable attention since the outbreak of the Covid19 pandemic in late 2019. Here we report the scalable expression and purification of soluble, immunologically active, SARS-CoV-2 Ncap in Escherichia coli. Codon-optimised synthetic genes encoding the original Ncap sequence and four common variants with an N-terminal 6His affinity tag (sequence MHHHHHHG) were cloned into an inducible expression vector carrying a regulated bacteriophage T5 synthetic promoter controlled by lac operator binding sites. The constructs were used to express Ncap proteins and protocols developed which allow efficient production of purified Ncap with yields of over 200 mg per litre of culture media. These proteins were deployed in ELISA assays to allow comparison of their responses to human sera. Our results suggest that there was no detectable difference between the 6His-tagged and untagged original Ncap proteins but there may be a slight loss of sensitivity of sera to other Ncap isolates. [ABSTRACT FROM AUTHOR]

Published
2024
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12. Synthesis and characterisation of M-doped titanium dioxide for the potential application in electrochemical devices

Author

Sayers, Laura R.

Subjects
621.31242
Abstract

The chemistry of systems intended to produce metal-doped, nanostructured oxide materials containing titanium(IV) has been investigated. Manganese and iron were chosen as cheap, sustainable metal atom dopants with a view to possible use of product materials in electrochemical devices. In particular, the aim was to enhance electronic conductivity and to introduce psudocapacitance for investigation in supercapacitor devices. Previous literature in this area was found to be misleading; products of earlier synthetic studies have been incompletely characterised, with solid products being assumed to be single crystalline phases and metal atom contents being assumed on the basis of hypothetical doping levels rather than being determined analytically. Electrochemical properties of product materials in electrode films were determined in this study via use of EIS, CV and galvanostatic charge-discharge techniques. Conventional sol-gel and micro-emulsion synthetic methods were undertaken with the view to control of product particle sizes and yields for single phase, Mn-doped titanium(lV) oxide (ideally within the anatase structure). Other than for the pure binary oxides of manganese or titanium, the products obtained were mixtures of oxides rather than single phases, with titanium(lV) in the rutile polymorph within the mixed-phase products. "Doped" product materials produced by the emulsion method showed no improvements in conductivity or specific capacitance when compared with a standard anatase (undoped) sample. The mixed-oxide materials produced by the sol-gel method, containing MnO2, had enhanced conductivities but no improvement in specific capacitance. Single-phase, anatase structured, Mn-doped titanium dioxide materials, were prepared using the continuous hydrothermal synthesis (CHS) method. The maximum amount of Mn successfully incorporated into the anatase structure was found to be approximately 8%wt, as determined by AAS, ICP-MS and SEM-EDX analytical methods; high Mn-content resulted in phase segregation and irreversibihty within cyclic voltammograms, as evident in observation of an oxidation peak but no reduction peak. The highest recorded specific capacitance value for these samples was that for the CHSMn008 sample (theoretical composition Ti0.92Mn0.08O2), with a value of 4.5 F g-1 (oxide specific surface area, 34.8 m2 g-1). This compared to a similarly prepared anatase (undoped) material with a specific surface area of 48.6 m2 g-1 and a specific capacitance of 2.5 F g-1. No correlation between specific surface areas and specific capacitance values was observed, but the BET-determined specific surface areas were for powdered samples whereas capacitances refer to composite electrode films. The initial CHS product materials displayed no improvement in conductivity, but pH-adjusted synthesis led to samples (denoted CHS MnKOH) with higher conductivities consequent on higher Mn contents, and these samples also had the highest specific capacitance values. In view of the success of the CHS method in producing Mn-doped anatase materials, it was also used to synthesise Fe-doped titanium(IV) oxides. The products were a range of single phase materials, but syntheses aimed at high Fe-contents led to mixtures, TiO2 + Fe oxides + Fe2TiOs- the maximum amount of Fe incorporated whilst still maintaining a pure single phase product was approximately 7%wt. The conductivity of these materials showed marked improvement relative to the standard (undoped) anatase sample, comparable to those for CHS MnKOH samples, but the Mn-doped samples had higher specific capacitances than Fe-doped counterparts. The highest specific capacitance values recorded were between 25 and 32 F g-1, regardless of dopant (Mn/Fe) and/or phase purity, and were associated with a high specific surface area or a high concentration of “dopant” within the sample.

Published
2012

14. Notes From the Field: Use of Emergency Medical Service Data to Augment COVID-19 Public Health Surveillance in Montgomery County, Maryland, From March to June 2020

Author

Sayers, David R, Hulse, Scott T, Webber, Bryant J, Burns, Timothy A, and Denicoff, Anne L

Subjects
Public aspects of medicine, RA1-1270
Abstract

Epidemiologic and syndromic surveillance metrics traditionally used by public health departments can be enhanced to better predict hospitalization for coronavirus disease (COVID-19). In Montgomery County, Maryland, measurements of oxygen saturation (SpO2) by pulse oximetry obtained by the emergency medical service (EMS) were added to these traditional metrics to enhance the public health picture for decision makers. During a 78-day period, the rolling 7-day average of the percentage of EMS patients with SpO2

Published
2020
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25. Characterisation of SARS-CoV-2 Membrane Protein-Specific Antibodies as an Additional Serological Target Following COVID-19 Infections Identified Through a High Content Microscopy-Based Platform

Author

Williams, Daniel Michael, primary, Hornsby, Hailey, additional, Shehata, Ola M., additional, Brown, Rebecca, additional, Gallis, Marta, additional, Meardon, Naomi, additional, Newman, Thomas A.H., additional, Plowright, Megan, additional, Zafred, Domen, additional, Shun-Shion, Amber S.M., additional, Hodder, Anthony J., additional, Bliss, Deepa, additional, Metcalfe, Andrew, additional, Edgar, James R., additional, Gordon, David E., additional, Sayers, Jon R., additional, Nicklin, Martin J.H., additional, Carroll, Miles W., additional, Consortium, PITCH, additional, Collini, Paul J., additional, Brown, Stephen, additional, de Silva, Thushan I., additional, and Peden, Andrew A., additional

Published
2023
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26. SARS-CoV-2 Incidence and Re-Infection During Delta and Omicron Ba.1 Waves in the Gambia: Results from a Prospective Household Cohort Study in a Population with Low SARS-CoV-2 Vaccine Coverage

Author

Jarju, Sheikh, primary, Wenlock, Rhys, additional, Danso, Madikoi, additional, Jobe, Dawda, additional, Jagne, Ya Jankey, additional, Darboe, Alansana, additional, Kumado, Michelle, additional, Jallow, Yusupha, additional, Touray, Mamlie, additional, Ceesay, Ebrima, additional, Gaye, Hoja, additional, Gaye, Biran, additional, Tunkara, Abdoulie, additional, Kandeh, Sheriff, additional, Gomes, Marie, additional, Sylva, Ellen-Lena, additional, Toure, Fatoumata, additional, Hornsby, Hailey, additional, Lindsey, Benjamin B., additional, Nicklin, Martin J.H., additional, Sayers, Jon R., additional, Sesay, Abdul K., additional, Kucharski, Adam J., additional, Hodgson, David, additional, Kampmann, Beate, additional, and de Silva, Thushan I., additional

Published
2023
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32. Risk factors for SARS-CoV-2 seroprevalence following the first pandemic wave in UK healthcare workers in a large NHS Foundation Trust

Author

Colton, Hayley, Hodgson, David, Hornsby, Hailey, Brown, Rebecca, Mckenzie, Joanne, Bradley, Kirsty L, James, Cameron, Lindsey, Benjamin B, Birch, Sarah, Marsh, Louise, Wood, Steven, Bayley, Martin, Dickson, Gary, James, David C, Nicklin, Martin J, Sayers, Jon R, Zafred, Domen, Rowland-Jones, Sarah L, Kudesia, Goura, Kucharski, Adam, CMMID COVID-19 Working Group, Darton, Thomas C, de Silva, Thushan I, and Collini, Paul J

Abstract

Background: We aimed to measure SARS-CoV-2 seroprevalence in a cohort of healthcare workers (HCWs) during the first UK wave of the COVID-19 pandemic, explore risk factors associated with infection, and investigate the impact of antibody titres on assay sensitivity. Methods: HCWs at Sheffield Teaching Hospitals NHS Foundation Trust were prospectively enrolled and sampled at two time points. We developed an in-house ELISA for testing participant serum for SARS-CoV-2 IgG and IgA reactivity against Spike and Nucleoprotein. Data were analysed using three statistical models: a seroprevalence model, an antibody kinetics model, and a heterogeneous sensitivity model. Results: Our in-house assay had a sensitivity of 99·47% and specificity of 99·56%. We found that 24·4% (n=311/1275) of HCWs were seropositive as of 12th June 2020. Of these, 39·2% (n=122/311) were asymptomatic. The highest adjusted seroprevalence was measured in HCWs on the Acute Medical Unit (41·1%, 95% CrI 30·0-52·9) and in Physiotherapists and Occupational Therapists (39·2%, 95% CrI 24·4-56·5). Older age groups showed overall higher median antibody titres. Further modelling suggests that, for a serological assay with an overall sensitivity of 80%, antibody titres may be markedly affected by differences in age, with sensitivity estimates of 89% in those over 60 years but 61% in those ≤30 years. Conclusions: HCWs in acute medical units and those working closely with COVID-19 patients were at highest risk of infection, though whether these are infections acquired from patients or other staff is unknown. Current serological assays may underestimate seroprevalence in younger age groups if validated using sera from older and/or more severe COVID-19 cases.

Published
2022

40. Intestinal Carriage of Verocytotoxigenic Escherichia coli O157, Salmonella, Thermophilic Campylobacter and Yersinia enterocolitica, in Cattle, Sheep and Pigs at Slaughter in Great Britain during 2003

Author

Milnes, A. S., Stewart, I., Clifton-Hadley, F. A., Davies, R. H., Newell, D. G., Sayers, A. R., Cheasty, T., Cassar, C., Ridley, A., Cook, A. J. C., Evans, S. J., Teale, C. J., Smith, R. P., McNally, A., Toszeghy, M., Futter, R., Kay, A., and Paiba, G. A.

Published
2008
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42. Author Reply to Peer Reviews of Centriole growth is not limited by a finite pool of components, but is limited by the Cdk1/Cyclin-dependent phosphorylation of Ana2/STIL

Author

Raff, Jordan W., primary, Lagerholm, B. Christoffer, additional, Sayers, Judith R., additional, van Houtum, Eline J.H., additional, Gartenmann, Lisa, additional, Saurya, Saroj, additional, Novak, Zsofia A., additional, Wong, Siu-Shing, additional, and Steinacker, Thomas L., additional

Published
2022
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45. A high content microscopy-based platform for detecting antibodies to the nucleocapsid, spike and membrane proteins of SARS-CoV-2

Author

Williams, Daniel M., primary, Hornsby, Hailey, additional, Shehata, Ola M., additional, Brown, Rebecca, additional, Zafred, Domen, additional, Shun-Shion, Amber S.M., additional, Hodder, Anthony J., additional, Bliss, Deepa, additional, Metcalfe, Andrew, additional, Edgar, James R., additional, Gordon, David E., additional, Sayers, Jon R., additional, Nicklin, Martin J., additional, Collini, Paul J., additional, Brown, Steve, additional, de Silva, Thushan I., additional, and Peden, Andrew A., additional

Published
2021
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