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17 results on '"Sayas, Enric"'

7. An Arabidopsis Mutant Over-Expressing Subtilase SBT4.13 Uncovers the Role of Oxidative Stress in the Inhibition of Growth by Intracellular Acidification

Author

Bissoli, Gaetano, primary, Muñoz-Bertomeu, Jesús, additional, Bueso, Eduardo, additional, Sayas, Enric, additional, Vilcara, Edgardo A., additional, Felipo, Amelia, additional, Niñoles, Regina, additional, Rubio, Lourdes, additional, Fernández, José A., additional, and Serrano, Ramón, additional

Published
2020
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11. Polyamines interfere with protein ubiquitylation and cause depletion of intracellular amino acids: a possible mechanism for cell growth inhibition.

Author

Sayas, Enric, Pérez‐Benavente, Beatriz, Manzano, Concepción, Farràs, Rosa, Alejandro, Santiago, Pozo, Juan Carlos, Ferrando, Alejandro, and Serrano, Ramón

Subjects
*AMINO acids, *POLYAMINES, *SACCHAROMYCES cerevisiae, *CELL proliferation, *UBIQUITINATION
Abstract

Spermidine is a polyamine present in eukaryotes with essential functions in protein synthesis. At high concentrations spermidine and norspermidine inhibit growth by unknown mechanisms. Transcriptomic analysis of the effect of norspermidine on the plant Arabidopsis thaliana indicates upregulation of the response to heat stress and denatured proteins. Accordingly, these polyamines inhibit protein ubiquitylation, both in vivo (in yeast, Arabidopsis, and human Hela cells) and in vitro (with recombinant ubiquitin ligase). This interferes with protein degradation by the proteasome, a situation known to deplete cells of amino acids. Norspermidine treatment of yeast cells induces amino acid depletion, and supplementation of media with amino acids counteracts growth inhibition and cellular amino acid depletion but not inhibition of protein polyubiquitylation. [ABSTRACT FROM AUTHOR]

Published
2019
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12. ARABIDOPSIS THALIANA HOMEOBOX25 Uncovers a Role for Gibberellins in Seed Longevity1[C][W]

Author

Bueso, Eduardo, Muñoz-Bertomeu, Jesús, Campos, Francisco, Brunaud, Veronique, Martínez, Liliam, Sayas, Enric, Ballester, Patricia, Yenush, Lynne, and Serrano, Ramón

Subjects
Genotype, Staining and Labeling, Arabidopsis Proteins, Gene Expression Profiling, Arabidopsis, food and beverages, Germination, Triazoles, Genes, Plant, Plants, Genetically Modified, Article, Gibberellins, Plant Mucilage, Phenotype, Gene Expression Regulation, Plant, Mutation, Seeds, Nucleotide Motifs, Promoter Regions, Genetic, Oligonucleotide Array Sequence Analysis
Abstract

Seed longevity is crucial for agriculture and plant genetic diversity, but it is limited by cellular damage during storage. Seeds are protected against aging by cellular defenses and by structures such as the seed coat. We have screened an activation-tagging mutant collection of Arabidopsis (Arabidopsis thaliana) and selected four dominant mutants with improved seed longevity (isl1-1D to isl4-1D) under both natural and accelerated aging conditions. In the isl1-1D mutant, characterized in this work, overexpression of the transcription factor ARABIDOPSIS THALIANA HOMEOBOX25 (ATHB25; At5g65410) increases the expression of GIBBERELLIC ACID3-OXIDASE2, encoding a gibberellin (GA) biosynthetic enzyme, and the levels of GA1 and GA4 are higher (3.2- and 1.4-fold, respectively) in the mutant than in the wild type. The morphological and seed longevity phenotypes of the athb25-1D mutant were recapitulated in transgenic plants with moderate (4- to 6-fold) overexpression of ATHB25. Simultaneous knockdown of ATHB25, ATHB22, and ATHB31 expression decreases seed longevity, as does loss of ATHB25 and ATHB22 function in a double mutant line. Seeds from wild-type plants treated with GA and from a quintuple DELLA mutant (with constitutive GA signaling) are more tolerant to aging, providing additional evidence for a role of GA in seed longevity. A correlation was observed in several genotypes between seed longevity and mucilage formation at the seed surface, suggesting that GA may act by reinforcing the seed coat. This mechanism was supported by the observation of a maternal effect in reciprocal crosses between the wild type and the athb25-1D mutant.

Published
2013

14. A forward genetic approach in Arabidopsis thaliana identifies a RING-type ubiquitin ligase as a novel determinant of seed longevity

Author

Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia, Ministerio de Ciencia e Innovación, Ministerio de Economía y Competitividad, Consejo Superior de Investigaciones Científicas, Bueso Ródenas, Eduardo, Ibanez, Carla, Sayas, Enric, Muñoz Bertomeu, Jesús, González Guzmán, Miguel, Rodríguez Egea, Pedro Luís, Serrano Salom, Ramón, Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia, Ministerio de Ciencia e Innovación, Ministerio de Economía y Competitividad, Consejo Superior de Investigaciones Científicas, Bueso Ródenas, Eduardo, Ibanez, Carla, Sayas, Enric, Muñoz Bertomeu, Jesús, González Guzmán, Miguel, Rodríguez Egea, Pedro Luís, and Serrano Salom, Ramón

Abstract

[EN] Seed longevity is important to preserve crops and wild plants and it is limited by progressive cellular damage (aging) during storage. The induction of cellular stress defenses and the formation of the seed coat are crucial protecting events during seed development, a process mediated in Arabidopsis thaliana by the transcription factors LEC1, LEC2, FUS3 and the abscisic acid-activated ABI3. In order to identify novel determinants of seed longevity we have screened an activation-tagging mutant collection of Arabidopsis and isolated a dominant mutant with increased seed longevity under both natural and accelerated aging conditions. Molecular characterization indicates that the mutant phenotype is caused by over-expression of the At2g26130 gene encoding a RING-type zinc finger putative ubiquitin ligase. Loss of function of this gene in a T-DNA insertion mutant resulted in decreased seed longevity. We named this important gene for seed longevity RSL1 (from Ring finger of Seed Longevity1) and we could demonstrate ubiquitin ligase activity with the recombinant protein. Morphological alterations in shoot tissues of the RSL1 over-expressing plants and analysis of gibberellins levels suggest that RSL1 may increase gibberellins responses by some unknown mechanism. These results validate the forward genetic approach to seed longevity and anticipate the identification of many novel determinants of this important trait. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

Published
2014

16. Toxicity, mutagenicity and transport in Saccharomyces cerevisiae of three popular DNA intercalating fluorescent dyes.

Author

Sayas, Enric, García‐López, Federico, and Serrano, Ramón

Abstract

We have compared the toxicity, mutagenicity and transport in Saccharomyces cerevisiae of three DNA-intercalating fluorescent dyes widely used to stain DNA in gels. Safety data about ethidium bromide (EtBr) are contradictory, and two compounds of undisclosed structure (Redsafe and Gelred) have been proposed as safe alternatives. Our results indicate that all three compounds inhibit yeast growth, with Gelred being the most inhibitory and also the only one causing cell death. EtBr and Gelred, but not Redsafe, induce massive formation of petite (non-respiratory) mutants, but only EtBr induces massive loss of mitochondrial DNA. All three compounds increase reversion of a chromosomal point mutation ( lys2-801amber), with Gelred being the most mutagenic and Redsafe the least. These dyes are all cationic and are probably taken by cells through non-selective cation channels. We could measure the glucose-energized transport of EtBr and Gelred inside the cells, while uptake of Redsafe was below our detection limit. We conclude that although all three compounds are toxic and mutagenic in the yeast system, Redsafe is the safest for yeast, probably because of very limited uptake by these cells. Copyright © 2015 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Published
2015
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