Search

Your search keyword '"Sawadkar, Prasad"' showing total 30 results
Start Over Author "Sawadkar, Prasad"
30 results on '"Sawadkar, Prasad"'

9. Engineering of a functional tendon

Author

Sawadkar, Prasad

Subjects
617.5
Abstract

Reconstruction of a tendon following rupture is surgically challenging as each end of the tendon retracts leaving a substantial gap so that direct repair is often not feasible. To restore function, a tendon graft is required to bridge this defect and presently these gaps are filled with auto-, allo- or, synthetic grafts but they all have clinical limitations. To overcome this problem, tissue engineered collagen grafts were developed by a rapid process using compressed cell seeded type I collagen. Previously these constructs were tested in vivo in intercostal spaces of a lapine model to test immunocompatibility but material properties of the tissue engineered collagen grafts are currently unsuitable to withstand complete load bearing in vivo. A modified suture technique was developed to physiologically withstand and off load the tissue engineered collagen graft to aid integration in vivo. This modified suture method allowed only partial load to be transferred onto the tissue engineered collagen graft. Lapine tendons were used to test mechanical strength of repairs and a stress model was built. The break point for modified suture technique with tissue engineered collagen graft in situ was significantly higher compared to standard modified Kessler suture technique. To test the effect of the partial load on tenocytes, mechanobiology was studied under static and 10% cyclic loads using custom designed tensioning culture force monitors with immunohistology and matrix remodelling gene expression as quantifiable outcomes. Tendon fibroblast seeded tissue engineered collagen grafts were tested in vivo in a lapine model up to 12 weeks without immobilization. The gross observation at 3 and 12 weeks showed bridged integration of the graft without any adhesion with significant increase in the mechanical properties for 3 and 12 weeks as compared to 1 week. Histological analysis showed that tendon fibroblasts from the native tendon were able to migrate into the graft with higher collagen remodelling and graft maturation at 12 weeks as compared to 3 and 1 week. Insertion of tissue engineered collagen graft using a novel load bearing suture technique which partially loads in vivo showed integration, greater mechanical strength and no adhesion formation in the time period tested and it has inherent advantages as compared to the present day tendon grafts.

Published
2015

11. sj-pptx-3-tej-10.1177_20417314211019238 – Research Data for Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration

Author

Sawadkar, Prasad, Mandakhbayar, Nandin, Patel, Kapil D, Buitrago, Jennifer Olmas, Kim, Tae Hyun, Rajasekar, Poojitha, Lali, Ferdinand, Kyriakidis, Christos, Rahmani, Benyamin, Mohanakrishnan, Jeviya, Dua, Rishbha, Greco, Karin, Lee, Jung-Hwan, Kim, Hae-Won, Knowles, Jonathan, and García – Gareta, Elena

Subjects
110599 Dentistry not elsewhere classified, FOS: Clinical medicine, FOS: Biological sciences, 69999 Biological Sciences not elsewhere classified
Abstract

Research Data, sj-pptx-3-tej-10.1177_20417314211019238 for Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration by Prasad Sawadkar, Nandin Mandakhbayar, Kapil D Patel, Jennifer Olmas Buitrago, Tae Hyun Kim, Poojitha Rajasekar, Ferdinand Lali, Christos Kyriakidis, Benyamin Rahmani, Jeviya Mohanakrishnan, Rishbha Dua, Karin Greco, Jung-Hwan Lee, Hae-Won Kim, Jonathan Knowles and Elena García – Gareta in Journal of Tissue Engineering

Published
2021
Full Text
View/download PDF

12. sj-pptx-2-tej-10.1177_20417314211019238 – Research Data for Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration

Author

Sawadkar, Prasad, Mandakhbayar, Nandin, Patel, Kapil D, Buitrago, Jennifer Olmas, Kim, Tae Hyun, Rajasekar, Poojitha, Lali, Ferdinand, Kyriakidis, Christos, Rahmani, Benyamin, Mohanakrishnan, Jeviya, Dua, Rishbha, Greco, Karin, Lee, Jung-Hwan, Kim, Hae-Won, Knowles, Jonathan, and García – Gareta, Elena

Subjects
110599 Dentistry not elsewhere classified, FOS: Clinical medicine, FOS: Biological sciences, 69999 Biological Sciences not elsewhere classified
Abstract

Research Data, sj-pptx-2-tej-10.1177_20417314211019238 for Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration by Prasad Sawadkar, Nandin Mandakhbayar, Kapil D Patel, Jennifer Olmas Buitrago, Tae Hyun Kim, Poojitha Rajasekar, Ferdinand Lali, Christos Kyriakidis, Benyamin Rahmani, Jeviya Mohanakrishnan, Rishbha Dua, Karin Greco, Jung-Hwan Lee, Hae-Won Kim, Jonathan Knowles and Elena García – Gareta in Journal of Tissue Engineering

Published
2021
Full Text
View/download PDF

13. sj-pptx-4-tej-10.1177_20417314211019238 – Research Data for Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration

Author

Sawadkar, Prasad, Mandakhbayar, Nandin, Patel, Kapil D, Buitrago, Jennifer Olmas, Kim, Tae Hyun, Rajasekar, Poojitha, Lali, Ferdinand, Kyriakidis, Christos, Rahmani, Benyamin, Mohanakrishnan, Jeviya, Dua, Rishbha, Greco, Karin, Lee, Jung-Hwan, Kim, Hae-Won, Knowles, Jonathan, and García – Gareta, Elena

Subjects
110599 Dentistry not elsewhere classified, FOS: Clinical medicine, FOS: Biological sciences, 69999 Biological Sciences not elsewhere classified
Abstract

Research Data, sj-pptx-4-tej-10.1177_20417314211019238 for Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration by Prasad Sawadkar, Nandin Mandakhbayar, Kapil D Patel, Jennifer Olmas Buitrago, Tae Hyun Kim, Poojitha Rajasekar, Ferdinand Lali, Christos Kyriakidis, Benyamin Rahmani, Jeviya Mohanakrishnan, Rishbha Dua, Karin Greco, Jung-Hwan Lee, Hae-Won Kim, Jonathan Knowles and Elena García – Gareta in Journal of Tissue Engineering

Published
2021
Full Text
View/download PDF

14. Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering

Author

Kohli, Nupur, Sharma, Vaibhav, Orera, Alodia, Sawadkar, Prasad, Owji, Nazanin, Frost, Oliver G, Bailey, Russell J., Snow, Martyn, Knowles, Jonathan C., Blunn, Gordon W., García-Gareta, Elena, Kohli, Nupur, Sharma, Vaibhav, Orera, Alodia, Sawadkar, Prasad, Owji, Nazanin, Frost, Oliver G, Bailey, Russell J., Snow, Martyn, Knowles, Jonathan C., Blunn, Gordon W., and García-Gareta, Elena

Abstract

Due to the limitations of bone autografts, we aimed to develop new composite biomaterials with pro-angiogenic and osteogenic properties to be used as scaffolds in bone tissue engineering applications. We used a porous, cross-linked and slowly biodegradable fibrin/alginate scaffold originally developed in our laboratory for wound healing, throughout which deposits of calcium phosphate (CaP) were evenly incorporated using an established biomimetic method. Material characterisation revealed the porous nature and confirmed the deposition of CaP precursor phases throughout the scaffolds. MC3T3-E1 cells adhered to the scaffolds, proliferated, migrated and differentiated down the osteogenic pathway during the culture period. Chick chorioallantoic membrane (CAM) assay results showed that the scaffolds were pro-angiogenic and biocompatible. The work presented here gave useful insights into the potential of these pro-angiogenic and osteogenic scaffolds for bone tissue engineering and merits further research in a pre-clinical model prior to its clinical translation.

Published
2021

16. Pre-screening the intrinsic angiogenic capacity of biomaterials in an optimised ex ovo chorioallantoic membrane model

Author

Kohli, Nupur, Sawadkar, Prasad, Ho, Sonia, Sharma, Vaibhav, Snow, Martyn, Powell, Sean, Woodruff, Maria A., Hook, Lilian, García-Gareta, Elena, Kohli, Nupur, Sawadkar, Prasad, Ho, Sonia, Sharma, Vaibhav, Snow, Martyn, Powell, Sean, Woodruff, Maria A., Hook, Lilian, and García-Gareta, Elena

Abstract

Biomaterial development for clinical applications is currently on the rise. This necessitates adequate in vitro testing, where the structure and composition of biomaterials must be specifically tailored to withstand in situ repair and regeneration responses for a successful clinical outcome. The chorioallantoic membrane of chicken embryos has been previously used to study angiogenesis, a prerequisite for most tissue repair and regeneration. In this study, we report an optimised ex ovo method using a glass-cling film set-up that yields increased embryo survival rates and has an improved protocol for harvesting biomaterials. Furthermore, we used this method to examine the intrinsic angiogenic capacity of a variety of biomaterials categorised as natural, synthetic, natural/synthetic and natural/natural composites with varying porosities. We detected significant differences in biomaterials’ angiogenesis with natural polymers and polymers with a high overall porosity showing a greater vascularisation compared to synthetic polymers. Therefore, our proposed ex ovo chorioallantoic membrane method can be effectively used to pre-screen biomaterials intended for clinical application.

Published
2020

19. Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration

Author

Sawadkar, Prasad, primary, Mandakhbayar, Nandin, additional, Patel, Kapil D, additional, Buitrago, Jennifer Olmas, additional, Kim, Tae Hyun, additional, Rajasekar, Poojitha, additional, Lali, Ferdinand, additional, Kyriakidis, Christos, additional, Rahmani, Benyamin, additional, Mohanakrishnan, Jeviya, additional, Dua, Rishbha, additional, Greco, Karin, additional, Lee, Jung-Hwan, additional, Kim, Hae-Won, additional, Knowles, Jonathan, additional, and García – Gareta, Elena, additional

Published
2021
Full Text
View/download PDF

27. A review on the use of cell therapy in the treatment of tendon disease and injuries

Author

Ho, Jasmine OY, Sawadkar, Prasad, and Mudera, Vivek

Subjects
lcsh:Biochemistry, cell culture, tendon injury, biocompatible materials, Tissue engineering, lcsh:QD415-436, Review, cell therapy
Abstract

Tendon disease and injuries carry significant morbidity worldwide in both athletic and non-athletic populations. It is estimated that tendon injuries account for 30%-50% of all musculoskeletal injuries globally. Current treatments have been inadequate in providing an accelerated process of repair resulting in high relapse rates. Modern concepts in tissue engineering and regenerative medicine have led to increasing interest in the application of cell therapy for the treatment of tendon disease. This review will explore the use of cell therapy, by bringing together up-to-date evidence from in vivo human and animal studies, and discuss the issues surrounding the safety and efficacy of its use in the treatment of tendon disease.

Published
2014

29. Ex Ovo Chorioallantoic Membrane Assay as a Model of Bone Formation by Biomaterials.

Author

Owji N, Kohli N, Frost OG, Sawadkar P, Snow M, Knowles JC, and García-Gareta E

Subjects
Animals, Chick Embryo, Alginates chemistry, Fibrin metabolism, Fibrin chemistry, Chorioallantoic Membrane metabolism, Chorioallantoic Membrane drug effects, Chorioallantoic Membrane blood supply, Osteogenesis drug effects, Osteogenesis physiology, Biocompatible Materials chemistry, Tissue Engineering methods, Calcium Phosphates chemistry, Calcium Phosphates metabolism, Tissue Scaffolds chemistry
Abstract

Biomaterials play an increasingly critical role in bone tissue engineering. However, achieving effective clinical translation requires a careful choice of biomimetic materials and thorough assessment of their efficacy and safety. Existing in vitro and in vivo models have drawbacks including time and cost constraints, invasive procedures, and discordance between animal models and clinical outcomes. Therefore, there is a demand for an alternative model. We hypothesized that the chick embryo chorioallantoic membrane can serve as a bioreactor to evaluate the initial sign of bone formation on scaffolds. In parallel, we investigated the osteogenic potential of a previously fabricated fibrin-alginate-calcium phosphate biomaterial (FACaP). Blood vessels were observed to infiltrate the scaffolds with early signs of bone formation, confirmed via RUNX-2 and alpha smooth muscle actin markers. The scaffolds' chemical composition was evaluated by Fourier-transform infrared spectroscopy, and ion chromatography was used to assess calcium ion release. Finally, the topography was examined by atomic force microscopy. In conclusion, this system offers simple refinement for in vivo models in bone tissue engineering and highlights the great potential of FACaP as an angiogenic and osteogenic biomaterial for non-load-bearing applications.

Published
2024
Full Text
View/download PDF

30. Development of a surgically optimized graft insertion suture technique to accommodate a tissue-engineered tendon in vivo.

Author

Sawadkar P, Alexander S, Tolk M, Wong J, McGrouther D, Bozec L, and Mudera V

Abstract

The traumatic rupture of tendons is a common clinical problem. Tendon repair is surgically challenging because the tendon often retracts, resulting in a gap between the torn end and its bony insertion. Tendon grafts are currently used to fill this deficit but are associated with potential complications relating to donor site morbidity and graft necrosis. We have developed a highly reproducible, rapid process technique to manufacture compressed cell-seeded type I collagen constructs to replace tendon grafts. However, the material properties of the engineered constructs are currently unsuitable to withstand complete load bearing in vivo. A modified suture technique has been developed to withstand physiological loading and off load the artificial construct while integration occurs. Lapine tendons were used ex vivo to test the strength of different suture techniques with different sizes of Prolene sutures and tissue-engineered collagen constructs in situ. The data were compared to standard modified Kessler suture using a standard tendon graft. Mechanical testing was carried out and a finite element analysis stress distribution model constructed using COMSOL 3.5 software. The break point for modified suture technique with a tissue-engineered scaffold was significantly higher (50.62 N) compared to a standard modified Kessler suture (12.49 N, p<0.05). Distributing suture tension further proximally and distally from the tendon ends increased the mechanical strength of the repairs. We now have ex vivo proof of concept that this suture technique is suitable for testing in vivo, and this will be the next stage of our research.

Published
2013
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results