188 results on '"Satoru Fujii"'
Search Results
2. Single cell analysis revealed that two distinct, unique CD4+ T cell subsets were increased in the small intestinal intraepithelial lymphocytes of aged mice
- Author
-
Yuki Yonemoto, Yasuhiro Nemoto, Ryo Morikawa, Nana Shibayama, Shigeru Oshima, Takashi Nagaishi, Tomohiro Mizutani, Go Ito, Satoru Fujii, and Ryuichi Okamoto
- Subjects
intraepithelial lymphocyte ,T cell aging ,intestinal immunity ,CD4 T cell ,scRNAseq ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Recent advances in research suggest that aging has a controllable chronic inflammatory disease aspect. Aging systemic T cells, which secrete pro-inflammatory factors, affect surrounding somatic cells, and accelerate the aging process through chronic inflammation, have attracted attention as potential therapeutic targets in aging. On the other hand, there are few reports on the aging of the intestinal immune system, which differs from the systemic immune system in many ways. In the current study, we investigated the age-related changes in the intestinal immune system, particularly in T cells. The most significant changes were observed in the CD4+ T cells in the small intestinal IEL, with a marked increase in this fraction in old mice and reduced expression of CD27 and CD28, which are characteristic of aging systemic T cells. The proliferative capacity of aging IEL CD4+ T cells was significantly more reduced than that of aging systemic T cells. Transcriptome analysis showed that the expression of inflammatory cytokines was not upregulated, whereas Cd8α, NK receptors, and Granzymes were upregulated in aging IEL CD4+ T cells. Functional analysis showed that aging IEL T cells had a higher cytotoxic function against intestinal tumor organoids in vitro than young IEL T cells. scRNAseq revealed that splenic T cells show a transition from naïve to memory T cells, whereas intestinal T cells show the emergence of a CD8αα+CD4+ T cell fraction in aged mice, which is rarely seen in young cells. Further analysis of the aging IEL CD4+ T cells showed that two unique subsets are increased that are distinct from the systemic CD4+ T cells. Subset 1 has a pro-inflammatory component, with expression of IFNγ and upregulation of NFkB signaling pathways. Subset 2 does not express IFNγ, but upregulates inhibitory molecules and nIEL markers. Expression of granzymes and Cd8a was common to both. These fractions were in opposite positions in the clustering by UMAP and had different TCR repertoires. They may be involved in the suppression of intestinal aging and longevity through anti-tumor immunity, elimination of senescent cells and stressed cells in the aging environment. This finding could be a breakthrough in aging research.
- Published
- 2024
- Full Text
- View/download PDF
3. Simultaneous Hybrid Transcatheter Aortic Valve Implantation and Endoscopic Mitral Valve RepairNovel Teaching Points
- Author
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Christina Oatway, MD, Junichi Shimamura, MD, Rodrigo Bagur, MD, PhD, Satoru Fujii, MD, and Michael W.A. Chu, MD, MEd
- Subjects
Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Published
- 2023
- Full Text
- View/download PDF
4. Method for evaluating the soundness of underground walls
- Author
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Takeshi Fujimori, Satoru Fujii, and Michito Shoji
- Subjects
existing pile ,pile integrity test ,reflected wave ,reuse ,soundness ,underground wall ,Architecture ,NA1-9428 ,Architectural engineering. Structural engineering of buildings ,TH845-895 - Abstract
Abstract The elastic wave caused by the input at the wallhead spreads throughout the plane of the wall, and thus, inspecting the soundness of underground walls using a pile integrity test (PIT) is a challenging task. This paper presents a feasible method for evaluating the soundness of underground walls via a PIT. In particular, by applying a low‐pass filter to the reflected wave recorded at the wall head, the wave components reflected from the wall ends and cracks are effectively separated from the components reflected from the wall sides. Hence, through the proposed strategy, the wave can be clearly identified for soundness evaluation.
- Published
- 2023
- Full Text
- View/download PDF
5. Management of a bi-caval dual lumen cannula clot obstruction after TEE guided diagnosis: a case report
- Author
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Raffael Pereira Cezar Zamper, Daniel Bainbridge, Dave Nagpal, and Satoru Fujii
- Subjects
Síndrome do desconforto respiratório agudo ,Catéteres, canulação ,Ecocardiografia, transesofageana ,Oxigenação por membrana extracorpórea ,Resolução de problemas ,Anesthesiology ,RD78.3-87.3 - Abstract
Background: Veno-venous extracorporeal membrane oxygenation is an established therapy for patients with refractory acute respiratory distress syndrome (ARDS). One complication related to the use of veno-venous extracorporeal membrane oxygenation is thrombosis despite proper anticoagulation. We report the diagnosis and management of a clot-obstruction in a single site cannula placed through the internal jugular vein, guided by transesophageal echocardiography. Case report: A 39 year-old male developed acute respiratory distress syndrome and hemodynamic instability after an episode of pulmonary aspiration in the ICU. Eight hours after placement of a single site veno-venous extracorporeal membrane oxygenation, suddenly the perfusionist noticed a reduction in flow. TEE showed a thrombus-like mass obstructing the inflow port in SVC and inflow at IVC was intact. After unsuccessful attempts to reposition the cannula, the team decided to insert additional femoral inflow cannula through the IVC. The single site catheter was then pulled out until its tip was positioned in the right atrium and all three ports of the catheter were switched to the infusion ports. After this, flows and oxygenation improved significantly. Unfortunately, despite all of the efforts, the patient died 2 days later. Discussion: The diagnosis of veno-venous extracorporeal membrane oxygenation cannula obstruction is based on reduced inflow rates, hemodynamic instability and poor oxygenation of blood. TEE allows evaluation of the flows inside the cannula and in this case, an obstruction was found. The management presented points to the fact that in a situation of catheter obstruction caused by a clot, there is a feasible alternative to assure minimal interruption of the hemodynamic support offered by the veno-venous extracorporeal membrane oxygenation. Resumo: Justificativa: A oxigenação por membrana extracorpórea veno-venosa é terapia estabelecida para pacientes com a síndrome do desconforto respiratório agudo. Uma complicação relacionada ao uso da oxigenação por membrana extracorpórea veno-venosa é trombose apesar de anti-coagulação adequada. Relatamos o diagnóstico e conduta em obstrução por coágulo em cânula de acesso único inserida pela veia jugular interna, guiada por ecocardiografia transesofageana. Relato de caso: Paciente do sexo masculino de 39 anos desenvolveu síndrome do desconforto respiratório agudo e instabilidade hemodinâmica após episódio de aspiração pulmonar na UTI. Oito horas após a instalação de oxigenação por membrana extracorpórea veno-venosa de acesso único, o perfusionista notou repentina redução no fluxo. A ETE revelou massa semelhante a um trombo obstruindo o portal de fluxo de entrada na VCS e o fluxo de saída na VCI estava intacto. Após tentativas sem sucesso para reposicionar a cânula, a equipe decidiu inserir cânula de entrada de fluxo adicional pela VCI. O catéter de acesso único foi então puxado até que sua ponta se posicionasse no átrio direito e todos os três portais do catéter fossem transferidos para os portais de infusão. A seguir, os fluxos e oxigenação melhoraram significativamente. Infelizmente, apesar dos esforços, o paciente foi a óbito 2 dias depois. Discussão: O diagnóstico de obstrução de cânula da oxigenação por membrana extracorpórea veno-venosa se baseia em velocidades reduzidas de entrada de fluxo, instabilidade hemodinâmica e oxigenação pobre do sangue. A ETE permite a avaliação dos fluxos dentro da cânula, e nesse caso foi encontrada obstrução. A técnica apresentada aponta para o fato de que em situação de obstrução de catéter causada por coágulo, existe alternativa factível para garantir interrrupção mínima do suporte hemodinâmico oferecido pela oxigenação por membrana extracorpórea veno-venosa.
- Published
- 2020
- Full Text
- View/download PDF
6. A fundamental study on the earthquake response reduction of base‐isolated foundation backfilled using an improved compound geomaterial
- Author
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Yuji Miyamoto, Atsushi Shimamura, Satoru Fujii, Fumiko Hoshizawa, and Hisatoshi Kashiwa
- Subjects
improved compound geomaterial ,nonlinear soil‐structure interaction ,seismic‐isolated foundation ,shaking table test ,three‐dimensional nonlinear finite element method ,Architecture ,NA1-9428 ,Architectural engineering. Structural engineering of buildings ,TH845-895 - Abstract
Abstract This study addresses the development of a seismic‐isolated foundation embedded with improved soil to reduce the response of a superstructure. The material properties of the new improved composite soil are investigated by performing laboratory tests. Subsequently, the response behaviors of test model structures are discussed considering various contact conditions at the bottom of the foundation and different improved backfill soils around the foundation by conducting shaking table tests. Furthermore, the results of the analysis are obtained using a three‐dimensional nonlinear finite element method to clarify the effect of contact conditions at the bottom of the foundation and that of the improved compound soil on the seismic response of a building. Based on these results, an effective method to reduce the response of a building is to decrease the frictional resistance of the foundation bottom in order to reduce the input motion and make it non‐resonant. In addition, the response can be reduced by absorbing the seismic vibration energy in the improved composite soil embedded around the foundation. Furthermore, it is expected that the improved composite soil along with its toughness will result in the suppression of the excessive deformation of the foundation due to the effect of embedding.
- Published
- 2018
- Full Text
- View/download PDF
7. Contribution of ATOH1+ Cells to the Homeostasis, Repair, and Tumorigenesis of the Colonic Epithelium
- Author
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Fumiaki Ishibashi, Hiromichi Shimizu, Toru Nakata, Satoru Fujii, Kohei Suzuki, Ami Kawamoto, Sho Anzai, Reiko Kuno, Sayaka Nagata, Go Ito, Tatsuro Murano, Tomohiro Mizutani, Shigeru Oshima, Kiichiro Tsuchiya, Tetsuya Nakamura, Mamoru Watanabe, and Ryuichi Okamoto
- Subjects
Atoh1 ,intestinal secretory cell ,plasticity ,de-differentiation ,intestinal stem cell ,colonic inflammation ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Summary: ATOH1 is a master transcription factor for the secretory lineage differentiation of intestinal epithelial cells (IECs). However, the comprehensive contribution of ATOH1+ secretory lineage IECs to the homeostasis, repair, and tumorigenesis of the intestinal epithelium remains uncertain. Through our ATOH1+ cell-lineage tracing, we show here that a definite number of ATOH1+ IECs retain stem cell properties and can form ATOH1+IEC-derived clonal ribbons (ATOH1+ICRs) under completely homeostatic conditions. Interestingly, colonic ATOH1+ IECs appeared to exhibit their stem cell function more frequently compared with those of the small intestine. Consistently, the formation of ATOH1+ICRs was significantly enhanced upon dextran sodium sulfate colitis-induced mucosal damage. In addition, colonic ATOH1+ IECs acquired tumor stem cell-like properties in the azoxymethane-DSS tumor model. Our results reveal an unexpected contribution of colonic ATOH1+ IECs to maintaining the stem cell population under both homeostatic and pathologic conditions and further illustrate the high plasticity of the crypt-intrinsic stem cell hierarchy.
- Published
- 2018
- Full Text
- View/download PDF
8. Data showing proliferation and differentiation of intestinal epithelial cells under targeted depletion of Notch ligands in mouse intestine
- Author
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Toru Nakata, Hiromichi Shimizu, Sayaka Nagata, Go Ito, Satoru Fujii, Kohei Suzuki, Ami Kawamoto, Fumiaki Ishibashi, Reiko Kuno, Sho Anzai, Tatsuro Murano, Tomohiro Mizutani, Shigeru Oshima, Kiichiro Tsuchiya, Tetsuya Nakamura, Katsuto Hozumi, Mamoru Watanabe, and Ryuichi Okamoto
- Subjects
Computer applications to medicine. Medical informatics ,R858-859.7 ,Science (General) ,Q1-390 - Abstract
The data on the immunohistochemical analysis of conditional Notch ligand knockout mice is presented. Targeted deletion of Jag1, Dll1, Dll4, or Dll1 plus Dll4 in Lgr5+ve cells was induced by a Cre-mediated gene recombination, and differentiation or proliferation of the intestinal epithelial cells was examined by immunohistochemistry. These data are the extension of the data presented and discussed in the paper entitled “Indispensable role of non-canonical Notch signaling in the proliferation of Apc-deficient intestinal tumors“ (Nakata et al., Submitted for publication) [1].
- Published
- 2017
- Full Text
- View/download PDF
9. Neural Bradley-Terry Rating: Quantifying Properties from Comparisons.
- Author
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Satoru Fujii
- Published
- 2024
- Full Text
- View/download PDF
10. Distinct expression patterns of Notch ligands, Dll1 and Dll4, in normal and inflamed mice intestine
- Author
-
Hiromichi Shimizu, Ryuichi Okamoto, Go Ito, Satoru Fujii, Toru Nakata, Kohei Suzuki, Tatsuro Murano, Tomohiro Mizutani, Kiichiro Tsuchiya, Tetsuya Nakamura, Katsuto Hozumi, and Mamoru Watanabe
- Subjects
Dll1 ,Dll4 ,Hes1 ,ATOH1 ,Notch signaling ,Intestinal epithelial cells ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Reports have suggested that the two Notch ligands, Dll1 and Dll4, are indispensable to maintain the homeostasis of the intestinal epithelium. However, within the intestinal epithelium, the precise distribution of the cells that express those ligands at the protein level remains largely unknown. Here, we show a series of immunohistochemical analysis through which we successfully identified mice intestinal epithelial cells (IECs) that endogenously express Dll1 or Dll4. Results showed that Dll1-positive (Dll1+ve) IECs reside exclusively within the crypt, whereas Dll4-positive (Dll4+ve) IECs can locate both in the crypt and in the villus of the small intestine. Also in the colon, Dll1+ve IECs resided at the lower part of the crypt, whereas Dll4+ve IECs resided at both upper and lower part of the crypt, including the surface epithelium. Both Dll1+ve and Dll4+ve IECs were ATOH1-positive, but Hes1-negative cells, and located adjacent to Hes1-positive cells within the crypts. A sub-population of both Dll1+ve and Dll4+ve IECs appeared to co-express Muc2, but rarely co-expressed other secretory lineage markers. However, as compared to Dll1+ve IECs, Dll4+ve IECs included larger number of Muc2-postive IECs, suggesting that Dll4 is more preferentially expressed by goblet cells. Also, we identified that Dll4 is expressed in the Paneth cells of the small intestine, whereas Dll1 and Dll4 is expressed in the c-kit-positive IECs of the colon, indicating that Dll1+ve and Dll4+ve IECs may contribute to constitute the intestinal stem cell niche. Compared to the normal colon, analysis of DSS-colitis showed that number of Dll1+ve IECs significantly decrease in the elongated crypts of the inflamed colonic mucosa. In sharp contrast, number of Dll4+ve IECs showed a significant increase in those crypts, which was accompanied by the increase in number of Hes1-positive IECs. Those Dll4+ve IECs were mostly found adjacent to the Hes1-positive IECs, suggesting that Dll4 may act as a major Notch ligand in the crypts of the inflamed colonic mucosa. Our results illustrate distinct expression patterns of Dll1 and Dll4 within the intestinal epithelium, and suggest that these two ligands may have different roles in normal and inflamed mucosa.
- Published
- 2014
- Full Text
- View/download PDF
11. Lineage-specific expression of bestrophin-2 and bestrophin-4 in human intestinal epithelial cells.
- Author
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Go Ito, Ryuichi Okamoto, Tatsuro Murano, Hiromichi Shimizu, Satoru Fujii, Toru Nakata, Tomohiro Mizutani, Shiro Yui, Junko Akiyama-Morio, Yasuhiro Nemoto, Eriko Okada, Akihiro Araki, Kazuo Ohtsuka, Kiichiro Tsuchiya, Tetsuya Nakamura, and Mamoru Watanabe
- Subjects
Medicine ,Science - Abstract
Intestinal epithelial cells (IECs) regulate the absorption and secretion of anions, such as HCO3(-) or Cl(-). Bestrophin genes represent a newly identified group of calcium-activated Cl(-) channels (CaCCs). Studies have suggested that, among the four human bestrophin-family genes, bestrophin-2 (BEST2) and bestrophin-4 (BEST4) might be expressed within the intestinal tissue. Consistently, a study showed that BEST2 is expressed by human colonic goblet cells. However, their precise expression pattern along the gastrointestinal tract, or the lineage specificity of the cells expressing these genes, remains largely unknown. Here, we show that BEST2 and BEST4 are expressed in vivo, each in a distinct, lineage-specific manner, in human IECs. While BEST2 was expressed exclusively in colonic goblet cells, BEST4 was expressed in the absorptive cells of both the small intestine and the colon. In addition, we found that BEST2 expression is significantly down-regulated in the active lesions of ulcerative colitis, where goblet cells were depleted, suggesting that BEST2 expression is restricted to goblet cells under both normal and pathologic conditions. Consistently, the induction of goblet cell differentiation by a Notch inhibitor, LY411575, significantly up-regulated the expression of not BEST4 but BEST2 in MUC2-positive HT-29 cells. Conversely, the induction of absorptive cell differentiation up-regulated the expression of BEST4 in villin-positive Caco-2 cells. In addition, we found that the up- or down-regulation of Notch activity leads to the preferential expression of either BEST4 or BEST2, respectively, in LS174T cells. These results collectively confirmed that BEST2 and BEST4 could be added to the lineage-specific genes of humans IECs due to their abilities to clearly identify goblet cells of colonic origin and a distinct subset of absorptive cells, respectively.
- Published
- 2013
- Full Text
- View/download PDF
12. Quantifying Feature Importance of Games and Strategies via Shapley Values.
- Author
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Satoru Fujii
- Published
- 2023
- Full Text
- View/download PDF
13. Deep Bradley-Terry Rating: Estimate Properties Without Metric of Unseen Items.
- Author
-
Satoru Fujii
- Published
- 2023
- Full Text
- View/download PDF
14. Management of a bi-caval dual lumen cannula clot obstruction after TEE guided diagnosis: a case report
- Author
-
Raffael Pereira Cezar Zamper, Daniel Bainbridge, Dave Nagpal, and Satoru Fujii
- Subjects
Acute respiratory distress syndrome ,Catheters, indwelling ,Echocardiography, transesophageal ,Extracorporeal membrane oxygenation ,Troubleshooting ,Anesthesiology ,RD78.3-87.3 - Abstract
Abstract Background: Veno-venous extracorporeal membrane oxygenation is an established therapy for patients with refractory acute respiratory distress syndrome (ARDS). One complication related to the use of veno-venous extracorporeal membrane oxygenation is thrombosis despite proper anticoagulation. We report the diagnosis and management of a clot-obstruction in a single site cannula placed through the internal jugular vein, guided by transesophageal echocardiography. Case report: A 39 year-old male developed acute respiratory distress syndrome and hemodynamic instability after an episode of pulmonary aspiration in the ICU. Eight hours after placement of a single site veno-venous extracorporeal membrane oxygenation, suddenly the perfusionist noticed a reduction in flow. TEE showed a thrombus-like mass obstructing the inflow port in SVC and inflow at IVC was intact. After unsuccessful attempts to reposition the cannula, the team decided to insert additional femoral inflow cannula through the IVC. The single site catheter was then pulled out until its tip was positioned in the right atrium and all three ports of the catheter were switched to the infusion ports. After this, flows and oxygenation improved significantly. Unfortunately, despite all of the efforts, the patient died 2 days later. Discussion: The diagnosis of veno-venous extracorporeal membrane oxygenation cannula obstruction is based on reduced inflow rates, hemodynamic instability and poor oxygenation of blood. TEE allows evaluation of the flows inside the cannula and in this case, an obstruction was found. The management presented points to the fact that in a situation of catheter obstruction caused by a clot, there is a feasible alternative to assure minimal interruption of the hemodynamic support offered by the veno-venous extracorporeal membrane oxygenation.
- Full Text
- View/download PDF
15. Single cell analysis revealed that two distinct, unique CD4+ T cell subsets were increased in the small intestinal intraepithelial lymphocytes of aged mice.
- Author
-
Yuki Yonemoto, Yasuhiro Nemoto, Ryo Morikawa, Nana Shibayama, Shigeru Oshima, Takashi Nagaishi, Tomohiro Mizutani, Go Ito, Satoru Fujii, and Ryuichi Okamoto
- Abstract
Recent advances in research suggest that aging has a controllable chronic inflammatory disease aspect. Aging systemic T cells, which secrete proinflammatory factors, affect surrounding somatic cells, and accelerate the aging process through chronic inflammation, have attracted attention as potential therapeutic targets in aging. On the other hand, there are few reports on the aging of the intestinal immune system, which differs from the systemic immune system in many ways. In the current study, we investigated the agerelated changes in the intestinal immune system, particularly in T cells. The most significant changes were observed in the CD4
+ T cells in the small intestinal IEL, with a marked increase in this fraction in old mice and reduced expression of CD27 and CD28, which are characteristic of aging systemic T cells. The proliferative capacity of aging IEL CD4+ T cells was significantly more reduced than that of aging systemic T cells. Transcriptome analysis showed that the expression of inflammatory cytokines was not upregulated, whereas Cd8a, NK receptors, and Granzymes were upregulated in aging IEL CD4+ T cells. Functional analysis showed that aging IEL T cells had a higher cytotoxic function against intestinal tumor organoids in vitro than young IEL T cells. scRNAseq revealed that splenic T cells show a transition from naïve to memory T cells, whereas intestinal T cells show the emergence of a CD8aa+CD4+ T cell fraction in aged mice, which is rarely seen in young cells. Further analysis of the aging IEL CD4+ T cells showed that two unique subsets are increased that are distinct from the systemic CD4+ T cells. Subset 1 has a pro-inflammatory component, with expression of IFNg and upregulation of NFkB signaling pathways. Subset 2 does not express IFNg, but upregulates inhibitory molecules and nIEL markers. Expression of granzymes and Cd8a was common to both. These fractions were in opposite positions in the clustering by UMAP and had different TCR repertoires. They may be involved in the suppression of intestinal aging and longevity through anti-tumor immunity, elimination of senescent cells and stressed cells in the aging environment. This finding could be a breakthrough in aging research. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
16. Does Near-Infrared Spectroscopy Serve as a Good Predictor of Outcome in Extracorporeal Cardiopulmonary Resuscitation?
- Author
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Satoru Fujii and Daniel Bainbridge
- Subjects
Anesthesiology and Pain Medicine ,Cardiology and Cardiovascular Medicine - Published
- 2023
17. EVALUATION METHOD OF UNDERGROUND WALL SOUNDNESS
- Author
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Takeshi FUJIMORI, Satoru FUJII, and Michito SHOJI
- Subjects
Architecture ,Building and Construction - Published
- 2022
18. Simultaneous Hybrid Transcatheter Aortic Valve Implantation and Endoscopic Mitral Valve Repair
- Author
-
Christina Oatway, Junichi Shimamura, Rodrigo Bagur, Satoru Fujii, and Michael W.A. Chu
- Subjects
Case Report ,Cardiology and Cardiovascular Medicine - Published
- 2022
19. Method for evaluating the soundness of underground walls
- Author
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Takeshi Fujimori, Satoru Fujii, and Michito Shoji
- Subjects
General Medicine - Published
- 2022
20. Development of E-Learning System Using Handwriting on Screen.
- Author
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Satoru Fujii, Rie Onishi, and Kouji Yoshida
- Published
- 2011
- Full Text
- View/download PDF
21. Trial of a Distance Learning System Using a Brain Wave Sensor.
- Author
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Kouji Yoshida, Yuuta Sakamoto, Yuuki Satou, Isao Miyaji, Kunihiro Yamada, and Satoru Fujii
- Published
- 2011
- Full Text
- View/download PDF
22. Ross procedure for acute infective endocarditis
- Author
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Junichi Shimamura, Paola Montanhesi, Satoru Fujii, Linrui Guo, and Michael W. A. Chu
- Subjects
Pulmonary and Respiratory Medicine ,Heart Valve Prosthesis Implantation ,Pulmonary Valve ,Treatment Outcome ,Endocarditis ,Aortic Valve ,Humans ,Surgery ,Endocarditis, Bacterial ,Cardiology and Cardiovascular Medicine ,Autografts ,Transplantation, Autologous - Abstract
Surgical treatment of infective endocarditis remains a challenge, with concerns of optimal prosthesis selection and risks of recurrent infection remaining paramount. The pulmonary autograft has unique features which may make it the ideal aortic valve substitute, especially in infectious endocarditis. We describe strategic considerations and technical details in performing a Ross procedure in a young patient with acute aortic valve endocarditis.
- Published
- 2022
23. Development and Evaluation of a Historical Tour Support System Using 3D Graphics and Mobile Terminal.
- Author
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Satoru Fujii, Takahiro Shima, Megumi Takahashi, and Koji Yoshida
- Published
- 2010
- Full Text
- View/download PDF
24. Distance Learning Support System for Game Programming with Java.
- Author
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Kouji Yoshida, Takumu Yaoi, Isao Miyaji, Kunihiro Yamada, and Satoru Fujii
- Published
- 2010
- Full Text
- View/download PDF
25. Development and Evaluation of a Ubiquitous Historical Tour Support System.
- Author
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Satoru Fujii, Yusuke Takahashi, Hisayoshi Kageyama, Hirokazu Aoyama, and Tadanori Mizuno
- Published
- 2007
- Full Text
- View/download PDF
26. Development of Ubiquitous Historical Tour Support System.
- Author
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Satoru Fujii, Yusuke Takahashi, Hisao Fukuoka, Teruhisa Ichikawa, Sanshiro Sakai, and Tadanori Mizuno
- Published
- 2005
- Full Text
- View/download PDF
27. Co2(CO)8-Catalyzed Reactions of Acetals or Lactones with Hydrosilanes and Carbon Monoxide. A New Access to the Preparation of 1,2-Diol Derivatives through Siloxymethylation
- Author
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Yasuhide Nakayama, Naoto Chatani, Yasuteru Kajikawa, Yoichi Kido, Shinji Murai, Yoshiya Fukumoto, Hideo Tokuhisa, and Satoru Fujii
- Subjects
Reaction conditions ,chemistry.chemical_compound ,Chemistry ,Diol ,Organic chemistry ,General Chemistry ,Vicinal ,Catalysis ,Carbon monoxide - Abstract
The Co2(CO)8-catalyzed reaction of acetals with hydrosilanes and CO under mild reaction conditions (an ambient temperature under an ambient CO pressure), leading to the production of vicinal diols ...
- Published
- 2021
28. Practice of Linux Lesson in Blended Learning.
- Author
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Kazuhiro Nakada, Tomonori Akutsu, Chris Walton, Satoru Fujii, Hiroshi Ichimura, Kunihiro Yamada, and Kouji Yoshida
- Published
- 2004
- Full Text
- View/download PDF
29. Development of a System for Learning Ecology Using 3D Graphics and XML.
- Author
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Satoru Fujii, Jun Iwata, Yuka Miura, Kouji Yoshida, Sanshiro Sakai, and Tadanori Mizuno
- Published
- 2004
- Full Text
- View/download PDF
30. A Trial of a Bidirectional Learning Management Tool for Promoting Learning by Mobile Phone.
- Author
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Kouji Yoshida, Kouiti Matsumoto, Kazuhiro Nakada, Tomonori Akutsu, Satoru Fujii, and Hiroshi Ichimura
- Published
- 2003
- Full Text
- View/download PDF
31. Development of a Remote Communication System for Computer Novices and Their Instructors.
- Author
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Satoru Fujii, Jun Iwata, Kouji Yoshida, and Tadanori Mizuno
- Published
- 2003
- Full Text
- View/download PDF
32. Newly developed atrial fibrillation in the perioperative setting: what to do next?
- Author
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Satoru FUJII
- Subjects
Anesthesiology and Pain Medicine ,Postoperative Complications ,Risk Factors ,Atrial Fibrillation ,Humans - Published
- 2022
33. 'Web-CALL': a language learning support system using Internet.
- Author
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Satoru Fujii, Jun Iwata, Mayumi Hattori, Mutsumi Iijima, and Tadanori Mizuno
- Published
- 2000
- Full Text
- View/download PDF
34. Conduta em obstrução por coágulo em cânula de duplo lúmen bicaval após diagnóstico guiado por ETE: relato de caso
- Author
-
Daniel Bainbridge, Dave Nagpal, Satoru Fujii, and Raffael Pereira Cezar Zamper
- Subjects
03 medical and health sciences ,0302 clinical medicine ,Anesthesiology and Pain Medicine ,030202 anesthesiology - Abstract
Resumo Justificativa A oxigenacao por membrana extracorporea veno‐venosa e terapia estabelecida para pacientes com a sindrome do desconforto respiratorio agudo. Uma complicacao relacionada ao uso da oxigenacao por membrana extracorporea veno‐venosa e trombose apesar de anti‐coagulacao adequada. Relatamos o diagnostico e conduta em obstrucao por coagulo em cânula de acesso unico inserida pela veia jugular interna, guiada por ecocardiografia transesofageana. Relato de caso Paciente do sexo masculino de 39 anos desenvolveu sindrome do desconforto respiratorio agudo e instabilidade hemodinâmica apos episodio de aspiracao pulmonar na UTI. Oito horas apos a instalacao de oxigenacao por membrana extracorporea veno‐venosa de acesso unico, o perfusionista notou repentina reducao no fluxo. A ETE revelou massa semelhante a um trombo obstruindo o portal de fluxo de entrada na VCS e o fluxo de saida na V C I estava intacto. Apos tentativas sem sucesso para reposicionar a cânula, a equipe decidiu inserir cânula de entrada de fluxo adicional pela VCI. O cateter de acesso unico foi, entao, puxado ate que sua ponta se posicionasse no atrio direito e todos os tres portais do cateter fossem transferidos para os portais de infusao. A seguir, os fluxos e oxigenacao melhoraram significativamente. Infelizmente, apesar dos esforcos, o paciente foi a obito 2 dias depois. Discussao O diagnostico de obstrucao de cânula da oxigenacao por membrana extracorporea veno‐venosa se baseia em velocidades reduzidas de entrada de fluxo, instabilidade hemodinâmica e oxigenacao pobre do sangue. A ETE permite a avaliacao dos fluxos dentro da cânula, e nesse caso foi encontrada obstrucao. A tecnica apresentada aponta para o fato de que em situacao de obstrucao de cateter causada por coagulo, existe alternativa factivel para garantir interrrupcao minima do suporte hemodinâmico oferecido pela oxigenacao por membrana extracorporea veno‐venosa.
- Published
- 2020
35. Endoscopic Repair of Obstructive Cor Triatriatum
- Author
-
Satoru Fujii, Brian Evans, Ali Hage, Ivan Iglesias, Byron H. Gottschalk, and Michael W.A. Chu
- Subjects
medicine.medical_specialty ,business.industry ,General Medicine ,medicine.disease ,Minimally invasive cardiac surgery ,Cor triatriatum sinister ,Surgery ,Endoscopic Cor Triatriatum ,Cor triatriatum ,medicine ,business ,ComputingMethodologies_COMPUTERGRAPHICS - Abstract
Graphical abstract, Highlights • CTS is characterized by a membrane that divides the left atrium into two chambers. • This patient also presented with mitral regurgitation. • The authors present multimodality images and intraoperative video clips. • In this case, repair was performed with a minimally invasive approach. • Intraoperative echocardiography is an essential element of patient care.
- Published
- 2019
36. Biofilm Formation and Virulence of Shigella flexneri Are Modulated by pH of Gastrointestinal Tract
- Author
-
Yi Wang, Phillip I. Tarr, Thaddeus S. Stappenbeck, Qiuhe Lu, Satoru Fujii, Brian D. Muegge, and I-Ling Chiang
- Subjects
Arginine ,Immunology ,Virulence ,medicine.disease_cause ,Microbiology ,Shigella flexneri ,medicine ,Secretion ,Shigella ,Cellular Microbiology: Pathogen-Host Cell Molecular Interactions ,Base Sequence ,biology ,Biofilm ,Hydrogen-Ion Concentration ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Small intestine ,Gastrointestinal Tract ,Infectious Diseases ,medicine.anatomical_structure ,Biofilms ,Parasitology ,Transcriptome ,Bacteria ,Deoxycholic Acid - Abstract
Shigella infection remains a public health problem in much of the world. Classic models of Shigella pathogenesis suggest that microfold epithelial cells in the small intestine are the preferred initial site of invasion. However, recent evidence supports an alternative model in which Shigella primarily infects a much wider range of epithelial cells that reside primarily in the colon. Here, we investigated whether the luminal pH difference between the small intestine and the colon could provide evidence in support of either model of Shigella flexneri pathogenesis. Because virulence factors culminating in cellular invasion are linked to biofilms in S. flexneri, we examined the effect of pH on the ability of S. flexneri to form and maintain adherent biofilms induced by deoxycholate. We showed that a basic pH (as expected in the small intestine) inhibited formation of biofilms and dispersed preassembled mature biofilms, while an acidic pH (similar to the colonic environment) did not permit either of these effects. To further elucidate this phenomenon at the molecular level, we probed the transcriptomes of biofilms and S. flexneri grown under different pH conditions. We identified specific amino acid (cysteine and arginine) metabolic pathways that were enriched in the bacteria that formed the biofilms but decreased when the pH increased. We then utilized a type III secretion system reporter strain to show that increasing pH reduced deoxycholate-induced virulence of S. flexneri in a dose-dependent manner. Taken together, these experiments support a model in which Shigella infection is favored in the colon because of the local pH differences in these organs.
- Published
- 2021
37. Hybrid Double Valve Replacement for Multivalvular Disease With Severe Mitral Annular Calcification
- Author
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Christopher Harle, Jill Gelinas, Michael W.A. Chu, Rodrigo Bagur, Satoru Fujii, and Mahmoud Alukayli
- Subjects
Pulmonary and Respiratory Medicine ,Cardiac Catheterization ,Mitral annular calcification ,medicine.medical_specialty ,medicine.medical_treatment ,Heart Valve Diseases ,Ventricular outflow tract obstruction ,macromolecular substances ,030204 cardiovascular system & hematology ,Prosthesis ,03 medical and health sciences ,0302 clinical medicine ,Mitral valve ,medicine ,Humans ,cardiovascular diseases ,Paravalvular leak ,Cardiac Surgical Procedures ,Groove (engineering) ,Aged ,Heart Valve Prosthesis Implantation ,Surgical approach ,business.industry ,Calcinosis ,General Medicine ,Surgery ,Treatment Outcome ,medicine.anatomical_structure ,030228 respiratory system ,Heart Valve Prosthesis ,cardiovascular system ,Mitral Valve ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Double valve replacement - Abstract
Severe mitral annular calcification poses a significant challenge, particularly in higher risk, frail, elderly patients with multivalvular disease. Both surgical and transcatheter options exist but remain fraught with risks of paravalvular leak, atrioventricular groove disruption, patient prosthesis mismatch, and left ventricular outflow tract obstruction. We present an innovative hybrid surgical approach to manage severe aortic and mitral valve disease in a Jehovah’s Witness patient with severe circumferential mitral annular calcification.
- Published
- 2021
38. Appropriate Clinical Trial Registration Is the Key to Transparent Reporting and Publication
- Author
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Satoru Fujii and Daniel Bainbridge
- Subjects
Clinical trial ,medicine.medical_specialty ,Anesthesiology and Pain Medicine ,business.industry ,medicine ,Key (cryptography) ,Humans ,Medical physics ,Registries ,Cardiology and Cardiovascular Medicine ,business - Published
- 2020
39. Biofilm Formation and Virulence of Shigella flexneri is Modulated by pH of Gastrointestinal Tract
- Author
-
Satoru Fujii, Brian D. Muegge, Yi Wang, I-Ling Chiang, Thaddeus S. Stappenbeck, and Qiuhe Lu
- Subjects
biology ,Arginine ,Chemistry ,Biofilm ,Virulence ,biology.organism_classification ,medicine.disease_cause ,Small intestine ,Microbiology ,Shigella flexneri ,medicine.anatomical_structure ,medicine ,Secretion ,Shigella ,Bacteria - Abstract
Shigella infections remain a major public health issue in developing countries. One model of Shigella pathogenesis suggests that the microfold epithelial cells in the small intestine are the preferred initial site of infection. However, a growing body of evidence supports an alternative model whereby Shigella primarily infects a much wider range of epithelial cells that reside primarily within the colon. Here, we investigated whether the luminal pH difference between the small intestine and colon could provide evidence in support of either model of Shigella flexneri pathogenesis. As virulence factors leading to cellular invasion are linked to biofilms in S. flexneri, we examined the effect of pH on S. flexneri’s ability to form and maintain adherent biofilms when induced by deoxycholate. We showed that a basic pH inhibited formation and dispersed pre-assembled mature biofilms while an acidic pH (similar to the colonic environment) did not have either of these effects. To further elucidate this phenomenon at the molecular level, we probed the transcriptomes of biofilms and S. flexneri grown in different pH conditions. We identified specific amino acid metabolic pathways (cysteine and arginine) that were enriched in the bacteria that formed the biofilms, but decreased upon pH increase. We then utilized a type III secretion system reporter strain to show that increasing pH reduced deoxycholate-induced virulence of S. flexneri in a dose dependent manner. Taken together, these experiments support a model whereby Shigella infection is favored in the colon because of the local pH differences in these organs.
- Published
- 2020
40. Anesthesia for Cardiac Ablation
- Author
-
Satoru Fujii, Achal Dhir, and Jian Ray Zhou
- Subjects
Sedation ,medicine.medical_treatment ,Hemodynamics ,Catheter ablation ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Anesthesia ,030212 general & internal medicine ,Intraoperative Care ,business.industry ,Cardiac Ablation ,Anesthesiologists ,Anesthesiology and Pain Medicine ,Perioperative care ,Anesthetic ,Catheter Ablation ,Breathing ,medicine.symptom ,Electrophysiologic Techniques, Cardiac ,Cardiology and Cardiovascular Medicine ,business ,medicine.drug - Abstract
As the complexity and duration of cardiac ablation procedures increase, there is a growing demand for anesthesiologist involvement in the electrophysiology suites for sedation and anesthesia provision, hemodynamic and neuromonitoring, and procedural guidance through transesophageal echocardiography. To deliver high-quality perioperative care, it is important that the anesthesiologist is intimately familiar with the evolving techniques and technologies, the anesthetic options and ventilation strategies, and the anticipated postprocedural complications.
- Published
- 2018
41. A fundamental study on the earthquake response reduction of base‐isolated foundation backfilled using an improved compound geomaterial
- Author
-
Hisatoshi Kashiwa, Atsushi Shimamura, Fumiko Hoshizawa, Yuji Miyamoto, and Satoru Fujii
- Subjects
Fundamental study ,Architectural engineering. Structural engineering of buildings ,Foundation (engineering) ,General Medicine ,three‐dimensional nonlinear finite element method ,NA1-9428 ,Reduction (complexity) ,Architecture ,TH845-895 ,improved compound geomaterial ,Geotechnical engineering ,seismic‐isolated foundation ,shaking table test ,Base (exponentiation) ,Geology ,nonlinear soil‐structure interaction - Abstract
This study addresses the development of a seismic‐isolated foundation embedded with improved soil to reduce the response of a superstructure. The material properties of the new improved composite soil are investigated by performing laboratory tests. Subsequently, the response behaviors of test model structures are discussed considering various contact conditions at the bottom of the foundation and different improved backfill soils around the foundation by conducting shaking table tests. Furthermore, the results of the analysis are obtained using a three‐dimensional nonlinear finite element method to clarify the effect of contact conditions at the bottom of the foundation and that of the improved compound soil on the seismic response of a building. Based on these results, an effective method to reduce the response of a building is to decrease the frictional resistance of the foundation bottom in order to reduce the input motion and make it non‐resonant. In addition, the response can be reduced by absorbing the seismic vibration energy in the improved composite soil embedded around the foundation. Furthermore, it is expected that the improved composite soil along with its toughness will result in the suppression of the excessive deformation of the foundation due to the effect of embedding.
- Published
- 2018
42. Single cell analysis of Crohn’s disease patient-derived small intestinal organoids reveals disease activity-dependent modification of stem cell properties
- Author
-
Hiromichi Shimizu, Sayaka Nagata, Konomi Kuwabara, Fumiaki Ishibashi, Ryuichi Okamoto, Satoru Fujii, Kento Takenaka, Tatsuro Murano, Ami Kawamoto, Reiko Kuno, Sho Anzai, Kiichiro Tsuchiya, Yui Hiraguri, Kazuo Ohtsuka, Kohei Suzuki, Minami Hama, Shiro Yui, Junichi Takahashi, Mamoru Watanabe, Toru Nakata, Tetsuya Nakamura, and Go Ito
- Subjects
0301 basic medicine ,Original Article—Alimentary Tract ,Crohn’s disease ,Balloon Enteroscopy ,Biopsy ,Gene Expression ,Biology ,Receptors, G-Protein-Coupled ,03 medical and health sciences ,Single-cell analysis ,Crohn Disease ,Gene expression ,Granulocyte Colony-Stimulating Factor ,Intestine, Small ,Organoid ,medicine ,Humans ,Solute Carrier Family 12, Member 2 ,Inflammation ,medicine.diagnostic_test ,Single cell analysis ,Regeneration (biology) ,Stem Cells ,Calcium-Binding Proteins ,Gastroenterology ,LGR5 ,Intestinal organoids ,Epithelial Cells ,Molecular biology ,Organoids ,030104 developmental biology ,Disease Progression ,Immunohistochemistry ,Stem cell ,Single-Cell Analysis ,Intestinal stem cell ,Transcriptome ,Biomarkers - Abstract
Background Intestinal stem cells (ISCs) play indispensable roles in the maintenance of homeostasis, and also in the regeneration of the damaged intestinal epithelia. However, whether the inflammatory environment of Crohn’s disease (CD) affects properties of resident small intestinal stem cells remain uncertain. Methods CD patient-derived small intestinal organoids were established from enteroscopic biopsy specimens taken from active lesions (aCD-SIO), or from mucosa under remission (rCD-SIO). Expression of ISC-marker genes in those organoids was examined by immunohistochemistry, and also by microfluid-based single-cell multiplex gene expression analysis. The ISC-specific function of organoid cells was evaluated using a single-cell organoid reformation assay. Results ISC-marker genes, OLFM4 and SLC12A2, were expressed by an increased number of small intestinal epithelial cells in the active lesion of CD. aCD-SIOs, rCD-SIOs or those of non-IBD controls (NI-SIOs) were successfully established from 9 patients. Immunohistochemistry showed a comparable level of OLFM4 and SLC12A2 expression in all organoids. Single-cell gene expression data of 12 ISC-markers were acquired from a total of 1215 cells. t-distributed stochastic neighbor embedding analysis identified clusters of candidate ISCs, and also revealed a distinct expression pattern of SMOC2 and LGR5 in ISC-cluster classified cells derived from aCD-SIOs. Single-cell organoid reformation assays showed significantly higher reformation efficiency by the cells of the aCD-SIOs compared with that of cells from NI-SIOs. Conclusions aCD-SIOs harbor ISCs with modified marker expression profiles, and also with high organoid reformation ability. Results suggest modification of small intestinal stem cell properties by unidentified factors in the inflammatory environment of CD. Electronic supplementary material The online version of this article (10.1007/s00535-018-1437-3) contains supplementary material, which is available to authorized users.
- Published
- 2018
43. Contribution of ATOH1+ Cells to the Homeostasis, Repair, and Tumorigenesis of the Colonic Epithelium
- Author
-
Tatsuro Murano, Ami Kawamoto, Shigeru Oshima, Tetsuya Nakamura, Kiichiro Tsuchiya, Sayaka Nagata, Fumiaki Ishibashi, Ryuichi Okamoto, Hiromichi Shimizu, Mamoru Watanabe, Go Ito, Reiko Kuno, Sho Anzai, Toru Nakata, Kohei Suzuki, Satoru Fujii, and Tomohiro Mizutani
- Subjects
0301 basic medicine ,ATOH1 ,medicine.disease_cause ,Biochemistry ,Mice ,0302 clinical medicine ,Basic Helix-Loop-Helix Transcription Factors ,Intestinal Mucosa ,lcsh:QH301-705.5 ,DSS ,lcsh:R5-920 ,Mice, Inbred BALB C ,biology ,AOM-DSS ,Intestinal epithelium ,Cell biology ,Neoplasm Proteins ,medicine.anatomical_structure ,Cell Transformation, Neoplastic ,030220 oncology & carcinogenesis ,Colonic Neoplasms ,Neoplastic Stem Cells ,Stem cell ,lcsh:Medicine (General) ,Colon ,intestinal stem cell ,Mice, Nude ,Mice, Transgenic ,digestive system ,Article ,colonic inflammation ,03 medical and health sciences ,intestinal secretory cell ,parasitic diseases ,Genetics ,medicine ,Animals ,Transcription factor ,Atoh1 ,de-differentiation ,Epithelial Cells ,Cell Biology ,Small intestine ,digestive system diseases ,tumorigenesis ,030104 developmental biology ,lcsh:Biology (General) ,plasticity ,biology.protein ,Carcinogenesis ,Azo Compounds ,Function (biology) ,Homeostasis ,Developmental Biology - Abstract
Summary ATOH1 is a master transcription factor for the secretory lineage differentiation of intestinal epithelial cells (IECs). However, the comprehensive contribution of ATOH1+ secretory lineage IECs to the homeostasis, repair, and tumorigenesis of the intestinal epithelium remains uncertain. Through our ATOH1+ cell-lineage tracing, we show here that a definite number of ATOH1+ IECs retain stem cell properties and can form ATOH1+IEC-derived clonal ribbons (ATOH1+ICRs) under completely homeostatic conditions. Interestingly, colonic ATOH1+ IECs appeared to exhibit their stem cell function more frequently compared with those of the small intestine. Consistently, the formation of ATOH1+ICRs was significantly enhanced upon dextran sodium sulfate colitis-induced mucosal damage. In addition, colonic ATOH1+ IECs acquired tumor stem cell-like properties in the azoxymethane-DSS tumor model. Our results reveal an unexpected contribution of colonic ATOH1+ IECs to maintaining the stem cell population under both homeostatic and pathologic conditions and further illustrate the high plasticity of the crypt-intrinsic stem cell hierarchy., Graphical Abstract, Highlights • Intestinal ATOH1+ cells can exhibit stem cell properties under homeostatic conditions • Recruitment of ATOH1+ cell-derived stem cells is enhanced by inflammation • Cell-intrinsic NF-kB signaling promotes generation of ATOH1+ cell-derived stem cells • ATOH1+ tumor stem cells contribute to the development of colitis-associated tumors, Ishibashi et al. report the contribution of ATOH1+ intestinal epithelial cells to the maintenance, regeneration, and tumorigenesis in the colon. They find that a definite number of ATOH1+ intestinal epithelial cells retain stem cell properties under homeostatic conditions. Also, generation of ATOH1+ cell-derived stem cells is significantly enhanced by the inflammatory environment and contributes to the development of colitis-associated tumors.
- Published
- 2017
44. Data showing proliferation and differentiation of intestinal epithelial cells under targeted depletion of Notch ligands in mouse intestine
- Author
-
Ryuichi Okamoto, Sho Anzai, Shigeru Oshima, Tomohiro Mizutani, Tatsuro Murano, Reiko Kuno, Fumiaki Ishibashi, Kiichiro Tsuchiya, Tetsuya Nakamura, Hiromichi Shimizu, Katsuto Hozumi, Go Ito, Ami Kawamoto, Sayaka Nagata, Satoru Fujii, Kohei Suzuki, Mamoru Watanabe, and Toru Nakata
- Subjects
0301 basic medicine ,Pathology ,medicine.medical_specialty ,JAG1 ,Multidisciplinary ,Notch signaling pathway ,LGR5 ,Biology ,lcsh:Computer applications to medicine. Medical informatics ,Genetic recombination ,Cell biology ,Mouse Intestine ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Knockout mouse ,medicine ,Immunohistochemistry ,lcsh:R858-859.7 ,Notch ligand ,lcsh:Science (General) ,Data Article ,lcsh:Q1-390 - Abstract
The data on the immunohistochemical analysis of conditional Notch ligand knockout mice is presented. Targeted deletion of Jag1, Dll1, Dll4, or Dll1 plus Dll4 in Lgr5+ve cells was induced by a Cre-mediated gene recombination, and differentiation or proliferation of the intestinal epithelial cells was examined by immunohistochemistry. These data are the extension of the data presented and discussed in the paper entitled "Indispensable role of non-canonical Notch signaling in the proliferation of Apc-deficient intestinal tumors" (Nakata et al., Submitted for publication) [1].
- Published
- 2017
45. Vasoplegia in Cardiac Surgery: Pave the Way for Prevention
- Author
-
Daniel Bainbridge and Satoru Fujii
- Subjects
Heart Failure ,medicine.medical_specialty ,Cardiopulmonary Bypass ,Mitral Valve Annuloplasty ,business.industry ,MEDLINE ,Mitral Valve Insufficiency ,medicine.disease ,law.invention ,Cardiac surgery ,Anesthesiology and Pain Medicine ,law ,Mitral valve annuloplasty ,Heart failure ,Internal medicine ,Vasoplegia ,medicine ,Cardiopulmonary bypass ,Cardiology ,Humans ,Cardiology and Cardiovascular Medicine ,business - Published
- 2019
46. A Curious Case of Blood-Culture-Negative Infective Endocarditis
- Author
-
Daniel Bainbridge, Elena Tugaleva, Satoru Fujii, and Michael W.A. Chu
- Subjects
medicine.medical_specialty ,business.industry ,Blood culture negative ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,Anesthesiology and Pain Medicine ,Internal medicine ,Infective endocarditis ,medicine ,030211 gastroenterology & hepatology ,030212 general & internal medicine ,Cardiology and Cardiovascular Medicine ,business - Published
- 2018
47. Ultrasound-guided cannulation: from its inception to future use
- Author
-
Satoru Fujii
- Subjects
medicine.medical_specialty ,Catheterization, Central Venous ,business.industry ,MEDLINE ,Ultrasound guided ,Anesthesiology and Pain Medicine ,medicine.artery ,Catheterization, Peripheral ,Radial Artery ,Medicine ,Radiology ,Radial artery ,Ultrasonography ,Cardiac Surgical Procedures ,business - Published
- 2019
48. Transversus thoracis muscle plane block and alternative techniques
- Author
-
Satoru Fujii
- Subjects
business.industry ,Plane (geometry) ,General Medicine ,Anatomy ,03 medical and health sciences ,0302 clinical medicine ,Anesthesiology and Pain Medicine ,030202 anesthesiology ,Block (telecommunications) ,Injection site ,Medicine ,Transversus thoracis muscle ,business ,030217 neurology & neurosurgery ,Acute pain - Abstract
To the editor In their letter to the editor in response to our article,[1][1] Garcia Simon et al [2][2] proposed that pecto-intercostal fascial block (PIFB) can be an alternative to the transversus thoracis muscle plane (TTP) block because of advantages such as superficial injection site and
- Published
- 2019
49. [Management of a bi-caval dual lumen cannula clot obstruction after TEE guided diagnosis: a case-report]
- Author
-
Raffael Pereira Cezar, Zamper, Daniel, Bainbridge, Dave, Nagpal, and Satoru, Fujii
- Subjects
Adult ,Male ,Respiratory Distress Syndrome ,Extracorporeal Membrane Oxygenation ,Cannula ,Humans ,Thrombosis ,Venae Cavae ,Echocardiography, Transesophageal - Abstract
Veno-venous extracorporeal membrane oxygenation is an established therapy for patients with refractory acute respiratory distress syndrome (ARDS). One complication related to the use of veno-venous extracorporeal membrane oxygenation is thrombosis despite proper anticoagulation. We report the diagnosis and management of a clot-obstruction in a single site cannula placed through the internal jugular vein, guided by transesophageal echocardiography.A 39 year-old male developed acute respiratory distress syndrome and hemodynamic instability after an episode of pulmonary aspiration in the ICU. Eight hours after placement of a single site veno-venous extracorporeal membrane oxygenation, suddenly the perfusionist noticed a reduction in flow. TEE showed a thrombus-like mass obstructing the inflow port in SVC and inflow at IVC was intact. After unsuccessful attempts to reposition the cannula, the team decided to insert additional femoral inflow cannula through the IVC. The single site catheter was then pulled out until its tip was positioned in the right atrium and all three ports of the catheter were switched to the infusion ports. After this, flows and oxygenation improved significantly. Unfortunately, despite all of the efforts, the patient died 2 days later.The diagnosis of veno-venous extracorporeal membrane oxygenation cannula obstruction is based on reduced inflow rates, hemodynamic instability and poor oxygenation of blood. TEE allows evaluation of the flows inside the cannula and in this case, an obstruction was found. The management presented points to the fact that in a situation of catheter obstruction caused by a clot, there is a feasible alternative to assure minimal interruption of the hemodynamic support offered by the veno-venous extracorporeal membrane oxygenation.
- Published
- 2019
50. Long-Term Culture Captures Injury-Repair Cycles of Colonic Stem Cells
- Author
-
Chin-Wen Lai, Feidi Chen, Aaron M. Ver Heul, Qiuhe Lu, Takahiro E. Ohara, Jiye Cheng, I-Ling Chiang, Renee Wu, Thaddeus S. Stappenbeck, Jeffrey I. Gordon, Satoru Fujii, Kelli L. VanDussen, Yi Wang, L. David Sibley, Nathan D. Han, Hana Janova, Ta-Chiang Liu, Charles E. Whitehurst, Shanshan Xiong, and Brian D. Muegge
- Subjects
Colon ,Population ,Cell Culture Techniques ,Inflammation ,Biology ,Models, Biological ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Stress, Physiological ,medicine ,Animals ,Regeneration ,Colitis ,education ,030304 developmental biology ,Homeodomain Proteins ,0303 health sciences ,education.field_of_study ,Regeneration (biology) ,Endoplasmic reticulum ,Stem Cells ,LGR5 ,Epithelial Cells ,3T3 Cells ,medicine.disease ,Cell biology ,Oxygen ,Unfolded protein response ,Stem cell ,medicine.symptom ,030217 neurology & neurosurgery - Abstract
The colonic epithelium can undergo multiple rounds of damage and repair, often in response to excessive inflammation. The responsive stem cell that mediates this process is unclear, in part because of a lack of in vitro models that recapitulate key epithelial changes that occur in vivo during damage and repair. Here, we identify a Hopx+ colitis-associated regenerative stem cell (CARSC) population that functionally contributes to mucosal repair in mouse models of colitis. Hopx+ CARSCs, enriched for fetal-like markers, transiently arose from hypertrophic crypts known to facilitate regeneration. Importantly, we established a long-term, self-organizing two-dimensional (2D) epithelial monolayer system to model the regenerative properties and responses of Hopx+ CARSCs. This system can reenact the “homeostasis-injury-regeneration” cycles of epithelial alterations that occur in vivo. Using this system, we found that hypoxia and endoplasmic reticulum stress, insults commonly present in inflammatory bowel diseases, mediated the cyclic switch of cellular status in this process.
- Published
- 2019
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