Search

Your search keyword '"Sasai, Kazumi"' showing total 304 results
Start Over Author "Sasai, Kazumi"
304 results on '"Sasai, Kazumi"'

8. Evaluation of the detection method by a flotation method using a wire loop for gastrointestinal parasites.

Author

Takano, Aruto, Morinaga, Daikichi, Teramoto, Isao, Hatabu, Toshimitsu, Kido, Yasutoshi, Kaneko, Akira, Hatta, Takeshi, Tsuji, Naotoshi, Uni, Shigehiko, Sasai, Kazumi, Katoh, Hiromitsu, and Matsubayashi, Makoto

Subjects
FLOTATION, OOCYSTS, CHICKENS, CENTRIFUGATION, EGGS
Abstract

Infections by gastrointestinal parasites are found in a variety of animals worldwide. For the diagnosis of such infections, the flotation method is commonly used to detect parasitic microorganisms, such as oocysts or eggs, in feces. Instead of adding a flotation solution after the final centrifugation step and using a cover slip to collect the parasites, the method using a wire loop for the recovery of the organisms has been reported as one of alternative methods. However, the recovery rates of microorganisms from the flotation method have not been analysed. In the present study, the utility of a flotation method with the use of a wire loop of 8 mm in diameter (the loop method) was evaluated using different numbers of E. tenella oocysts and Heterakis gallinarum eggs, and chicken fecal samples collected at the farms. Consequently, we found that the oocysts and eggs in tubes could be collected at a ratio of 2.00 to 3.08. Thus, our results indicate that the loop method is a simple and time saving method, implicating the application for the estimated OPG/ EPG (Oocysts/Eggs per gram) of the samples. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

32. Phylogenetic characterization of Isospora jaracimrmani oocysts from a veiled chameleon (family Chamaeleonidae; Chamaeleo calyptratus) reared at a zoo in Ishikawa, Japan

Author

EKAWASTI, Fitrine, KITAGAWA, Kazuya, DOMAE, Hiroshi, WARDHANA, April Hari, NAGASAWA, Junki, SHIBAHARA, Tomoyuki, TOKORO, Masaharu, SASAI, Kazumi, and MATSUBAYASHI, Makoto

Subjects
Feces, Isospora, Japan, parasitic diseases, Oocysts, Animals, Wildlife Science, Lizards, Note, oocyst, veiled chameleon, Phylogeny
Abstract

Oocysts of Isospora sp. were detected in the feces of a veiled chameleon (family Chamaeleonidae; Chamaeleo calyptratus) kept at a zoo in Ishikawa, Japan. Phylogenetic analysis placed the sequence in the cluster of Isospora spp. isolated from reptiles. Based on a comparison of morphological data of ten previously reported Isospora species from the Chamaeleonidae family, this isolate was morphologically similar to I. jaracimrmani, which has been considered to be a virulent species. This case study suggests the possibility that species of Isospora might not always cause disease because the animal that shed these oocysts showed no symptoms for more than two months.

Published
2021

35. Swine multifocal ulcerative colitis and crypt abscesses associated with Entamoeba polecki subtype 3 and Salmonella Typhimurium

Author

ITO, Hirotaka, HOSOKAWA, Kumiko, KAWAMURA, Midori, ITO, Naomi, ABETO, Yusuke, MATSUBAYASHI, Makoto, SASAI, Kazumi, and SHIBAHARA, Tomoyuki

Subjects
Diarrhea, Salmonella typhimurium, Swine Diseases, Salmonella Infections, Animal, Entamoebiasis, Coinfection, Swine, animal diseases, Salmonella enterica serovar Typhimurium, multifocal ulcerative colitis, bacterial infections and mycoses, Note, post-weaning pig, digestive system diseases, Abscess, Entamoeba, fluids and secretions, Entamoeba polecki subtype 3, Japan, Pathology, Animals, Colitis, Ulcerative
Abstract

Piglets aged approximately 50 days exhibited diarrhea and wasting. Multiple white foci were detected in the colon of a dead piglet; histopathological findings revealed multifocal ulcers and crypt abscesses with Entamoeba trophozoites and gram-negative bacilli in the piglet. These pathogens were identified as Entamoeba polecki subtype 3 and Salmonella enterica serovar Typhimurium, respectively. Numerous E. polecki subtype 3 trophozoites were located on the edge of the ulcerative and necrotic lesions in the lamina propria. Crypt abscesses were associated with S. Typhimurium. These results suggest that E. polecki subtype 3 caused multifocal ulcerative colitis accompanied by crypt abscesses with S. Typhimurium in the piglet. This study is the first report of colitis with E. polecki subtype 3 and S. Typhimurium coinfection.

Published
2020

41. Distribution of Eimeria uekii and Eimeria raichoi in cage protection environments for the conservation of Japanese rock ptarmigans (Lagopus muta japonica) in the Japanese Alps

Author

Matsubayashi, Makoto, primary, Kobayashi, Atsushi, additional, Kaneko, Minemitsu, additional, Kinoshita, Moemi, additional, Tsuchida, Sayaka, additional, Shibahara, Tomoyuki, additional, Hasegawa, Masami, additional, Nakamura, Hiroshi, additional, Sasai, Kazumi, additional, and Ushida, Kazunari, additional

Published
2021
Full Text
View/download PDF

48. Parasitic development in intestines and oocyst shedding patterns for infection by Eimeria uekii and Eimeria raichoi in Japanese rock ptarmigans, Lagopus muta japonica, protected by cages in the Southern Japanese Alps

Author

Matsubayashi, Makoto, primary, Kinoshita, Moemi, additional, Kobayashi, Atsushi, additional, Tsuchida, Sayaka, additional, Shibahara, Tomoyuki, additional, Hasegawa, Masami, additional, Nakamura, Hiroshi, additional, Sasai, Kazumi, additional, and Ushida, Kazunari, additional

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results