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Background: Because chondrocyte viability is imperative for successful osteochondral allograft transplantation, sterilization techniques must provide antimicrobial effects with minimal cartilage toxicity. Chlorhexidine gluconate (CHG) is an effective disinfectant; however, its use with human articular cartilage requires further investigation. Purpose: To determine the maximal chlorhexidine concentration that does not affect chondrocyte viability in allografts and to determine whether this concentration effectively sterilizes contaminated osteoarticular grafts. Study Design: Controlled laboratory study. Methods: Osteochondral plugs were subjected to pulse lavage with 1-L solutions of 0.002%, 0.01%, 0.05%, and 0.25% CHG and cultured for 0, 1, 2, and 7 days in media of 10% fetal bovine serum and antibiotics. Chondrocyte viability was determined via LIVE/DEAD Viability Assay. Plugs were contaminated with Staphylococcus aureus and randomized to 4 treatment groups. One group was not contaminated; the 3 others were contaminated and received no treatment, saline pulse lavage, or saline pulse lavage with 0.002% CHG. Serial dilutions were plated and colony-forming units assessed. Results: The control group and the 0.002% CHG group showed similar cell viability, ranging from 67% ± 4% to 81% ± 22% (mean ± SD) at all time points. In the 0.01% CHG group, cell viability was reduced in comparison with control by 2-fold at day 2 and remained until day 7 ( P < .01). The 0.05% and 0.25% CHG groups showed a 2-fold reduction in cell viability at day 1 ( P < .01). At day 7, cell viability was reduced to 15% ± 18% (4-fold decrease) for the 0.05% CHG group and 10% ± 19% (6-fold decrease) for the 0.25% CHG group ( P < .01). Contaminated grafts treated with 0.002% CHG demonstrated no colony-forming units. Conclusion: Pulse lavage with 0.002% CHG does not cause significant cell death within 7 days after exposure, while CHG at concentrations >0.002% significantly decreases chondrocyte viability within 1 to 2 days after exposure and should therefore not be used for disinfection of osteochondral allograft. Pulse lavage does not affect chondrocyte viability but cannot be used in isolation to sterilize contaminated fragments. Overall, 0.002% CHG was shown to effectively decontaminate osteoarticular fragments. Clinical Relevance: This study offers a scientific protocol for sterilizing osteochondral fragments that does not adversely affect cartilage viability.

This study reports the long-term effectiveness of meniscal allograft transplantation performed by a single senior operating surgeon. In this study 22 meniscus transplantations in 22 patients were evaluated at a minimum of 7-year follow-up (mean 8.5 +/- 1.3 years) using standardized scoring scales. Subgroup analysis was performed to stratify outcomes based on medial (59.1%) versus lateral (40.9%) meniscus transplantation, and transplantations performed in isolation (36.4%) versus those performed concomitantly (63.6%). Patients reported significant improvement in all scoring scales (p < 0.05). Average satisfaction was 8.8 out of 10. All the patients were completely or mostly satisfied with the results of their surgery. Overall subjective knee condition improved from 3.5 to 6.9 (p < 0.05). Patients undergoing medial compartment repair and combined transplantations reported greater improvement from baseline to followup than did their counterparts. An overall success rate of 88% was found for all patients at the final follow-up. Based on this data, meniscal allograft transplantation is a viable treatment option for meniscal-deficient patients in reducing pain, increasing range of motion, and improving patient function and satisfaction at a minimum of 7 years postsurgery.

Purpose: The purpose of this study was to report the clinical outcomes of autologous chondrocyte implantation (ACI) procedures performed by a single orthopedic surgeon at a minimum of 7 years follow-up. Methods: A retrospective review of prospectively collected data was performed on 29 patients who underwent ACI of the knee between the years of 1998 and 2003. Prospective data were collected to assess changes in standardized outcome measures preoperatively and 2, 4, and 7 years postoperatively. All patients enrolled in the study were also recruited to undergo physical examination when possible. Results: The final cohort consisted of 29 patients with a mean final follow-up time of 8.40 years (range = 7.14-10.88 years). Comparing preoperative scores to 7-year postoperative values, the mean International Knee Documentation Committee (IKDC) score improved from 39.80 to 59.24 ( P < 0.001), mean Tegner-Lysholm score increased from 48.07 to 74.17 ( P < 0.001), SF-12 physical score improved from 40.38 to 48.66 ( P < 0.001), and SF-12 mental score improved from 44.14 to 48.98 ( P < 0.05). Significant improvement occurred in Knee Injury and Osteoarthritis Outcome Score (KOOS) pain (56.03 to 80.36), symptoms (54.19 to 74.75), activities of daily living (72.01 to 85.90), sports (23.34 to 55.34), and quality of life (24.56 to 56.03) ( P < 0.001). In addition, 7-year postoperative scores were at or near levels seen at 2 years (mean = 2.16; range = 0.94-4.03 years) and 4 years (mean = 4.43; range = 2.16-5.88 years) postoperatively, reflecting durable improvement. Subjectively, on a scale of 1 to 10 (10 being completely satisfied), the mean postoperative satisfaction rate was 8.14. Additionally, 88.9% of the patients would elect to have this surgery again if the same problem was to occur in the contralateral joint. Conclusions: The results of ACI in patients who present with symptomatic, full-thickness chondral defects remain durable at a minimum of 7-year follow-up with persistent, high levels of patient satisfaction. Level of Evidence: Case series; Level of evidence, IV.

The complex structure of articular cartilage allows for diverse knee function throughout range of motion and weight bearing. However, disruption to the structural integrity of the articular surface can cause significant morbidity. Due to an inherently poor regenerative capacity, articular cartilage defects present a treatment challenge for physicians and therapists. For many patients, a trial of nonsurgical treatment options is paramount prior to surgical intervention. In instances of failed conservative treatment, patients can undergo an array of palliative, restorative, or reparative surgical procedures to treat these lesions. Palliative methods include debridement and lavage, while restorative techniques include marrow stimulation. For larger lesions involving subchondral bone, reparative procedures such as osteochondral grafting or autologous chondrocyte implantation are considered. Clinical success not only depends on the surgical techniques but also requires strict adherence to rehabilitation guidelines. The purpose of this article is to review the basic science of articular cartilage and to provide an overview of the procedures currently performed at our institution for patients presenting with symptomatic cartilage lesions.

Background: Joint injections and aspirations are used to reduce joint pain and decrease inflammation. The efficacy of these injections is diminished when they are placed inadvertently in the wrong location or compartment. The purpose of this study was to determine whether the use of varying sites or imaging techniques affects the rate of accurate needle placement in aspiration and injection in the shoulder, elbow, and knee. Hypotheses: (1) Accuracy rates of different joint injection sites will demonstrate variability. (2) Injection accuracy rates will be improved when performed with concomitant imaging. Study Design: Systematic review of the literature. Methods: Studies reporting injection accuracy based on image verification were identified through a systematic search of the English literature. Accuracy rates were compared for currently accepted injection sites in the shoulder, elbow, and knee. In addition, accuracy rates with and without imaging of these joints were compared. Results: In the glenohumeral joint, there is a statistically higher accuracy rate with the posterior approach when compared with the anterior approach (85% vs 45%). Injection site selection did not affect accuracy for the subacromial space, acromioclavicular joint, elbow, or knee. The use of imaging improved injection accuracy in the glenohumeral joint (95% vs 79%), subacromial space (100% vs 63%), acromioclavicular joint (100% vs 45%), and knee (99% vs 79%). Conclusion: Injection accuracy rates are significantly higher for the posterior approach compared with the anterior approach for the glenohumeral joint. Similarly, the accuracy rates are also higher when imaging is used in conjunction with injection of the glenohumeral joint, subacromial space, acromioclavicular joint, and knee.

Articular cartilage is susceptible to damage; however, it has limited capacity for repair. Damage can lead to persistent symptoms including pain, swelling, and loss of function and may ultimately progress to symptomatic degeneration of the joint. To restore function and minimize symptoms, many advocate surgical intervention in selected candidates, which can range from arthroscopic debridement to restorative procedures depending on patient and lesion characteristics. Autologous Chondrocyte Implantation (ACI) is a two-stage, typically second-line intervention where cultured autologous chondrocytes are used with the aim of resurfacing symptomatic chondral defects with hyaline or hyaline-like cartilage. Careful patient selection is important. We present an overview of this procedure including indications and contraindications, surgical technique, and post-operative management. A review of published ACI outcomes is then presented.

Objective: Because P188 poloxamer is effective in promoting cell survival in models of acute trauma, the objectives were to understand the mechanism of its action focusing on glycogen synthase kinase-3 (GSK3) activation, interleukin-6 (IL-6), and p38 signaling. Design: Sixteen normal human tali were impacted using a 4-mm diameter indenter with an impulse of 1 Ns. Eight-millimeter cartilage plugs containing the 4-mm impacted core and 4-mm adjacent nonimpacted ring were removed and cultured with or without P188. Cell lysates were analyzed using Western blots with antibodies against total and phosphorylated extracellular signal-regulated protein kinase (ERK), c-Jun NH(2)-terminal kinase (JNK), p38, ATF-2, GSK3, Stat1, and Stat3. Additional tests were performed with the p38 inhibitor (p38i) SB203580. Results: Studied pathways were activated after impaction with the peak of activity at 1 hour. P188 completely attenuated phosphorylation of Statl and ATF-2 and inhibited p38, Stat3, JNK, ERK, and GSK3. The p38i partially offset phosphorylation of Stat3, GSK3, and ERK suggesting a role of p38 in these three pathways. Additionally, the p38i improved cell survival (P = 0.053) and reduced apoptosis (by approximately 20%, P = 0.046, versus almost 40% by P188), thus confirming that P188 acts (at least in part) through the p38 pathway. Conclusion: Our results report a novel mechanism through which P 188 exerts its protective effects on cartilage in the model of acute injury. In addition to its effect on cellular membrane, P188 affects stress-related p38 signaling, apoptosis-related GSK3, and inflammation-related IL-6 signaling. Taken together, these findings suggest that P188 alone or in combination with proanabolic agents may have a therapeutic potential in preventing progressive cartilage degeneration and the development of posttraumatic osteoarthritis.

The treatment of cartilage injuries secondary to patellar instability is multifactorial. These factors include the time frame of patellar instability, the pathoanatomic factors, the extent of symptoms, and the size and location of the cartilage injury. The chondral or osteochondral injury which occurs with acute patellar instability is managed with either reduction and internal fixation or removal. The treatment of the patellar instability component is controversial in the acute setting, especially when there is no need for an osteochondral surgery. In such cases, patellar dislocations are treated nonoperatively. However, if the patient is indicated for surgery for treatment of osteochondral fracture and intra-operatively the medial patellofemoral ligament (MPFL) remains pathologically lax and presents with a discrete MPFL injury site, there is the option of acute repair. Chondral injury associated with recurrent patellar instability may be subdivided into 2 overlapping groups: (1) chondral damage resulting from the instability episodes (distal medial region of the patellar and/or lateral proximal region of the lateral femoral condyle) and (2) chondrosis secondary to the chronic lateral tracking of the patella (chronic patellar subluxation) from patholaxity of the MPFL (lateral chondrosis to patella and trochlea in addition to the injuries sustained from the actual instability episodes). The treatment is based on the patient's chief complaints and the unique underlying pathoanatomy. Many of these patients will have one or more predisposing factors, such as patella alta, trochlear dysplasia, patholaxity of the MPFL and an excessively lateral position of the tibial tuberosity. Therefore, the key to managing this patient population is to address each patient's specific pathoanatomy, which by definition will include addressing the MPFL patholaxity. The goals are to stabilize the patella, normalize patellofemoral contact area and/or forces, and then restore the articular cartilage based on relevant symptoms. As of this writing, treatment options in the United States include debridement, marrow stimulation, osteochondral auto and allograft, autologous chondrocyte implantation, and the use of particulate articular cartilage.

Articular cartilage is susceptible to damage; however, it has limited capacity for repair. Damage can lead to persistent symptoms including pain, swelling, and loss of function and may ultimately progress to symptomatic degeneration of the joint. To restore function and minimize symptoms, many advocate surgical intervention in selected candidates, which can range from arthroscopic debridement to restorative procedures depending on patient and lesion characteristics. Autologous Chondrocyte Implantation (ACI) is a two-stage, typically second-line intervention where cultured autologous chondrocytes are used with the aim of resurfacing symptomatic chondral defects with hyaline or hyaline-like cartilage. Careful patient selection is important. We present an overview of this procedure including indications and contraindications, surgical technique, and post-operative management. A review of published ACI outcomes is then presented.

The treatment of patellofemoral (PF) articular cartilage pathology is difficult because the pathology is multifactorial and symptoms related to the cartilage portion of the problem are expressed through noncartilaginous tissue. Since cartilage is aneural, the symptoms from the cartilage defects are mediated through the mechanical effects on bone and by cartilage debris that causes noxious stimulation of soft tissues. A systematic history, thorough physical examination, and appropriate imaging studies are essential to assign a portion of the patient’s symptoms to the cartilage pathology. A successful outcome for patellofemoral articular cartilage restoration requires treatment of each individual component of the multifactorial pathology, and with careful patient selection and comprehensive patient and knee specific surgical management, it is possible for cartilage restoration in the patellofemoral compartment to have a success rate similar to the tibiofemoral compartment.

Multiple case series have reported outcomes of the AMZ procedures. Despite the heterogeneity in outcome measurements, results demonstrate high percentages of excellent and good results and improvements in objective, subjective and functional measures. Attention to details related to surgical planning and properly managing patient expectations is most likely to lead to good or excellent results. Newer techniques (i.e., the T3 system) allow the surgeon to objectively determine the inclination of the osteotomy to properly restore patellofemoral mechanics based upon the preoperative planning.

Context: Platelet-rich plasma (PRP) may affect soft tissue healing via growth factors released after platelet degranulation. Because of this potential benefit, clinicians have begun to inject PRP for the treatment of tendon, ligament, muscle, and cartilage injuries and early osteoarthritis. Evidence Acquisition: A PubMed search was performed for studies relating to PRP, growth factors, and soft tissue injuries from 1990 to 2010. Relevant references from these studies were also retrieved. Results: Soft tissue injury is a major source of disability that may often be complicated by prolonged and incomplete recovery. Numerous growth factors may potentiate the healing and regeneration of tendons and ligaments. The potential benefits of biologically enhanced healing processes have led to a recent interest in the use of PRP in orthopaedic sports medicine. There has been widespread anecdotal use of PRP for muscle strains, tendinopathy, and ligament injuries and as a surgical adjuvant to rotator cuff repair, anterior cruciate ligament reconstruction, and meniscal or labral repairs. Although the fascination with this emerging technology has led to a dramatic increase in its use, scientific data supporting this use are still in their infancy. Conclusions: The literature is replete with studies on the basic science of growth factors and their relation to the maintenance, proliferation, and regeneration of various tissues and tissue-derived cells. Despite the promising results of several animal studies, well-controlled human studies are lacking.

Introduction Meniscal allograft transplantation (MAT) has been hypothesized as a treatment option for young, meniscus-deficient patients. The long-term efficacy of this procedure is lacking in literature; thus, the purpose of this study is to report the long-term results (minimum 7 years postoperative) of meniscal allograft transplantation. Methods A retrospective review of prospectively collected data with a minimum 7-year follow-up having undergone MAT by a single surgeon (senior author) between November 1997 and July 2002 was performed. Three patients (10.3%) in this cohort had a failed allograft and went on to have a subsequent intervention and were excluded from further analysis. Preoperative subjective data and physical examination findings were compared to the follow-up findings. When possible, a radiographic evaluation of the knee was obtained and assessed for bone plug/bridge healing and joint space integrity. Patient outcome evaluations were performed using the SF-12 Physical/Mental, Lysholm, IKDC, and the KOOS (Pain, Symptom, Activities of Daily Living, Sports, and Quality of Life) scores. In addition, patients were asked to rate their overall surgical satisfaction and whether the patient would have the surgery performed on the contralateral knee given similar circumstances. Finally, preselected subgroup analysis was also performed. Results Twenty-two patients met the inclusion criteria with a mean follow-up of 8.49 years. Postoperative validated subjective scores showed statistically significant improvement from mean preoperative values. The mean postoperative satisfaction rate was 8.80 out of 10. Moreover, 90.9% of the patients stated that they would elect to have this surgery again. In addition, all measures exhibited sustained improvements in the 2-, 4-, and 7-year postoperative states as compared to the preoperative states (Table 1). Subgroup analysis of meniscal allograft transplantation performed concomitantly with other procedures (n=14) and those performed in isolation (n = 8) showed increased scores from mean preoperative to follow-up values on all knee scoring scales. No statistical difference was found on subjective data when comparing patients above (n=14) and below (n=8) a BMI of 25 kg/m 2 . There were, also, no significant differences between failed allograft meniscus transplantations and successful allograft meniscus transplantations with respect to subjective preoperative parameters. Follow-up physical examination revealed minimal patellofemoral joint tenderness and effusion with near-normal range of motion. Radiographic evaluation demonstrated consistent successful bony integration of the bone plugs/bridge from the allograft meniscus. Conclusion The patient population reported improvement on all validated surveys postoperatively. Additional analysis of our cohort demonstrated maintained levels of improvement at 7 years postoperatively when compared to 2- and 4-year follow-up scores. This data suggests that meniscal allograft transplantation is a durable treatment option for meniscus deficient patients, as it is effective in reducing pain, increasing range of motion and improving joint function in the long term. Data collected on the 3 reported allograft transplantation failures demonstrated that patient anatomy and lifestyle play an important role in allowing for an optimal outcome. While the results of this long-term study are encouraging, further research is needed to better assess the extent that allograft meniscus transplantation has in preventing the progression of osteoarthritis and maintaining an improved level of pain and function.