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15 results on '"Sarris, Alexi J."'

2. Mechanism based heparanase inhibitors reduce cancer metastasis in vivo

Author

Boer, Casper de, Armstrong, Zachary, Lit, Vincent A. J., Barash, Uri, Ruijgrok, Gijs, Boyango, Ilanit, Weitzenberg, Merle M., Schröder, Sybrin P., Sarris, Alexi J. C., Meeuwenoord, Nico J., Bule, Pedro, Kayal, Yasmine, Ilan, Neta, Codée, Jeroen D. C., Vlodavsky, Israel, Overkleeft, Herman S., Davies, Gideon J., and Wu, Liang

Published
2022

3. Live‐Cell Imaging of Sterculic Acid—a Naturally Occurring 1,2‐Cyclopropene Fatty Acid—by Bioorthogonal Reaction with Turn‐On Tetrazine‐Fluorophore Conjugates**

Author

Bertheussen, Kristine, primary, van de Plassche, Merel, additional, Bakkum, Thomas, additional, Gagestein, Berend, additional, Ttofi, Iakovia, additional, Sarris, Alexi J. C., additional, Overkleeft, Herman S., additional, van der Stelt, Mario, additional, and van Kasteren, Sander I., additional

Published
2022
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4. Mechanism-based heparanase inhibitors reduce cancer metastasis in vivo

Author

de Boer, Casper, primary, Armstrong, Zachary, additional, Lit, Vincent A. J., additional, Barash, Uri, additional, Ruijgrok, Gijs, additional, Boyango, Ilanit, additional, Weitzenberg, Merle M., additional, Schröder, Sybrin P., additional, Sarris, Alexi J. C., additional, Meeuwenoord, Nico J., additional, Bule, Pedro, additional, Kayal, Yasmine, additional, Ilan, Neta, additional, Codée, Jeroen D. C., additional, Vlodavsky, Israel, additional, Overkleeft, Herman S., additional, Davies, Gideon J., additional, and Wu, Liang, additional

Published
2022
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6. Fluorogenic Bifunctional trans ‐Cyclooctenes as Efficient Tools for Investigating Click‐to‐Release Kinetics

Author

Geus, Mark A. R., primary, Maurits, Elmer, additional, Sarris, Alexi J. C., additional, Hansen, Thomas, additional, Kloet, Max S., additional, Kamphorst, Kiki, additional, Hoeve, Wolter, additional, Robillard, Marc S., additional, Pannwitz, Andrea, additional, Bonnet, Sylvestre A., additional, Codée, Jeroen D. C., additional, Filippov, Dmitri V., additional, Overkleeft, Herman S., additional, and Kasteren, Sander I., additional

Published
2020
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8. Chemical Control over T-Cell Activation in Vivo Using Deprotection of trans-Cyclooctene-Modified Epitopes

Author

van der Gracht, Anouk M. F., primary, de Geus, Mark A. R., additional, Camps, Marcel G. M., additional, Ruckwardt, Tracy J., additional, Sarris, Alexi J. C., additional, Bremmers, Jessica, additional, Maurits, Elmer, additional, Pawlak, Joanna B., additional, Posthoorn, Michelle M., additional, Bonger, Kimberly M., additional, Filippov, Dmitri V., additional, Overkleeft, Herman S., additional, Robillard, Marc S., additional, Ossendorp, Ferry, additional, and van Kasteren, Sander I., additional

Published
2018
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10. Chemical Control over T-Cell Activation in VivoUsing Deprotection of trans-Cyclooctene-Modified Epitopes

Author

van der Gracht, Anouk M. F., de Geus, Mark A. R., Camps, Marcel G. M., Ruckwardt, Tracy J., Sarris, Alexi J. C., Bremmers, Jessica, Maurits, Elmer, Pawlak, Joanna B., Posthoorn, Michelle M., Bonger, Kimberly M., Filippov, Dmitri V., Overkleeft, Herman S., Robillard, Marc S., Ossendorp, Ferry, and van Kasteren, Sander I.

Abstract

Activation of a cytotoxic T-cell is a complex multistep process, and tools to study the molecular events and their dynamics that result in T-cell activation in situand in vivoare scarce. Here, we report the design and use of conditional epitopes for time-controlled T-cell activation in vivo. We show that trans-cyclooctene-protected SIINFEKL (with the lysine amine masked) is unable to elicit the T-cell response characteristic for the free SIINFEKL epitope. Epitope uncaging by means of an inverse-electron demand Diels–Alder (IEDDA) event restored T-cell activation and provided temporal control of T-cell proliferation in vivo.

Published
2018
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13. Live-Cell Imaging of Sterculic Acid-a Naturally Occurring 1,2-Cyclopropene Fatty Acid-by Bioorthogonal Reaction with Turn-On Tetrazine-Fluorophore Conjugates.

Author

Bertheussen K, van de Plassche M, Bakkum T, Gagestein B, Ttofi I, Sarris AJC, Overkleeft HS, van der Stelt M, and van Kasteren SI

Subjects
Cycloaddition Reaction, Cyclopropanes, Fatty Acids, Monounsaturated, Fluorescent Dyes chemistry, Ionophores, Fatty Acids, Heterocyclic Compounds chemistry
Abstract

In the field of lipid research, bioorthogonal chemistry has made the study of lipid uptake and processing in living systems possible, whilst minimising biological properties arising from detectable pendant groups. To allow the study of unsaturated free fatty acids in live cells, we here report the use of sterculic acid, a 1,2-cyclopropene-containing oleic acid analogue, as a bioorthogonal probe. We show that this lipid can be readily taken up by dendritic cells without toxic side effects, and that it can subsequently be visualised using an inverse electron-demand Diels-Alder reaction with quenched tetrazine-fluorophore conjugates. In addition, the lipid can be used to identify changes in protein oleoylation after immune cell activation. Finally, this reaction can be integrated into a multiplexed bioorthogonal reaction workflow by combining it with two sequential copper-catalysed Huisgen ligation reactions. This allows for the study of multiple biomolecules in the cell simultaneously by multimodal confocal imaging., (© 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.)

Published
2022
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14. Fluorogenic Bifunctional trans-Cyclooctenes as Efficient Tools for Investigating Click-to-Release Kinetics.

Author

de Geus MAR, Maurits E, Sarris AJC, Hansen T, Kloet MS, Kamphorst K, Ten Hoeve W, Robillard MS, Pannwitz A, Bonnet SA, Codée JDC, Filippov DV, Overkleeft HS, and van Kasteren SI

Abstract

The inverse electron demand Diels-Alder pyridazine elimination reaction between tetrazines and allylic substituted trans-cyclooctenes (TCOs) is a key player in bioorthogonal bond cleavage reactions. Determining the rate of elimination of alkylamine substrates has so far proven difficult. Here, we report a fluorogenic tool consisting of a TCO-linked EDANS fluorophore and a DABCYL quencher for accurate determination of both the click and release rate constants for any tetrazine at physiologically relevant concentrations., (© 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.)

Published
2020
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15. Fast and pH-Independent Elimination of trans-Cyclooctene by Using Aminoethyl-Functionalized Tetrazines.

Author

Sarris AJC, Hansen T, de Geus MAR, Maurits E, Doelman W, Overkleeft HS, Codée JDC, Filippov DV, and van Kasteren SI

Abstract

The inverse-electron-demand Diels-Alder/pyridazine elimination tandem reaction, in which the allylic substituent on trans-cyclooctene is eliminated following reaction with tetrazines, is gaining interest as a versatile bioorthogonal process. One potential shortcoming of such currently used reactions is their propensity to proceed faster and more efficiently at lower pH, a feature caused by the nature of the tetrazines used. Here, we present aminoethyl-substituted tetrazines as the first pH-independent reagents showing invariably fast elimination kinetics at all biologically relevant pH values., (© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2018
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