29 results on '"Sarr AD"'
Search Results
2. A metagenomic assessment of bacterial community in spices sold open-air markets in Saint-Louis, Senegal.
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Sané S, Diouara AAM, Coundoul S, Tene SD, Kane A, Wade SF, Tamba A, Diop M, Mbaye MN, Thiam F, Dieng M, Mbengue M, Nguer CM, Sarr AD, Ndao AS, and Touré Kane C
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- Senegal, Bacteria genetics, Bacteria classification, Bacteria isolation & purification, Humans, Metagenome, Microbiota genetics, Curcuma genetics, Curcuma microbiology, Spices microbiology, Metagenomics methods, RNA, Ribosomal, 16S genetics
- Abstract
Natural spices play an essential role in human nutrition and well-being. However, their processing on different scales can expose them to potential sources of contamination. This study aimed to describe the bacterial community genomic footprint in spices sold in Senegal. Spice samples were collected in August 2022 in Saint-Louis, Senegal. The genomic region coding bacterial 16S rRNA was then amplified and sequenced using Oxford Nanopore Technology (ONT). Sequencing was carried out on two batches of samples, one containing part of the "Local Spices or Herbs" (n = 10), and the other, a mixture of 7 spices, Curcuma, Thyme and the other part of the "Local Spices or Herbs" (n = 39). Results showed high bacterial diversity and the predominance of Escherichia coli and Salmonella enterica in samples, with total reads of 65,744 and 165,325 for the two batches, respectively. The sample category "Homemade mixture of food condiments ", which includes all "Local Spices or Herbs" samples, showed remarkable bacterial diversity. These were followed by Curcuma, a blend of 7 spices and thyme. Also, the different categories of spices studied show similarities in their bacterial composition. These results highlight the microbial community's highly diverse genomic profile, including pathogenic bacteria, in spice samples., (© 2024. The Author(s).)
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- 2024
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3. Time to deliver: report of the WHO Independent High-Level Commission on NCDs.
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Nishtar S, Niinistö S, Sirisena M, Vázquez T, Skvortsova V, Rubinstein A, Mogae FG, Mattila P, Ghazizadeh Hashemi SH, Kariuki S, Narro Robles J, Adewole IF, Sarr AD, Gan KY, Piukala SM, Al Owais ARBM, Hargan E, Alleyne G, Alwan A, Bernaert A, Bloomberg M, Dain K, Frieden T, Patel VH, Kennedy A, and Kickbusch I
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- Advisory Committees, Health Policy, Humans, Mental Disorders epidemiology, Noncommunicable Diseases epidemiology, World Health Organization, Global Health, Health Priorities, International Cooperation, Mental Disorders therapy, Noncommunicable Diseases therapy
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- 2018
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4. Building laboratory capacity to support HIV care in Nigeria: Harvard/APIN PEPFAR, 2004-2012.
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Hamel DJ, Sankalé JL, Samuels JO, Sarr AD, Chaplin B, Ofuche E, Meloni ST, Okonkwo P, and Kanki PJ
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Introduction: From 2004-2012, the Harvard/AIDS Prevention Initiative in Nigeria, funded through the US President's Emergency Plan for AIDS Relief programme, scaled up HIV care and treatment services in Nigeria. We describe the methodologies and collaborative processes developed to improve laboratory capacity significantly in a resource-limited setting. These methods were implemented at 35 clinic and laboratory locations., Methods: Systems were established and modified to optimise numerous laboratory processes. These included strategies for clinic selection and management, equipment and reagent procurement, supply chains, laboratory renovations, equipment maintenance, electronic data management, quality development programmes and trainings., Results: Over the eight-year programme, laboratories supported 160 000 patients receiving HIV care in Nigeria, delivering over 2.5 million test results, including regular viral load quantitation. External quality assurance systems were established for CD4+ cell count enumeration, blood chemistries and viral load monitoring. Laboratory equipment platforms were improved and standardised and use of point-of-care analysers was expanded. Laboratory training workshops supported laboratories toward increasing staff skills and improving overall quality. Participation in a World Health Organisation-led African laboratory quality improvement system resulted in significant gains in quality measures at five laboratories., Conclusions: Targeted implementation of laboratory development processes, during simultaneous scale-up of HIV treatment programmes in a resource-limited setting, can elicit meaningful gains in laboratory quality and capacity. Systems to improve the physical laboratory environment, develop laboratory staff, create improvements to reduce costs and increase quality are available for future health and laboratory strengthening programmes. We hope that the strategies employed may inform and encourage the development of other laboratories in resource-limited settings.
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- 2015
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5. N,N'-Dimethyl-ethylenediammonium dioxalatocuprate(II).
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Gaye PA, Sarr AD, Gaye M, Sanselme M, and Agasse PV
- Abstract
The asymmetric unit of the title salt, (C(4)H(14)N(2))[Cu(C(2)O(4))(2)], consists of one complex anion and two cationic half-mol-ecules, the other halves being generated by inversion symmetry. The Cu(II) atom in the anion is coordinated by two bidentate oxalate ligands in a distorted square-planar geometry. Inter-molecular hydrogen bonds, involving the NH groups as donors and O atoms as acceptors, are observed, which lead to the formation of a three-dimensional network structure.
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- 2011
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6. 1-(Thio-phen-2-yl)ethanone thio-semi-carbazone.
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Gaye PA, Sy A, Sarr AD, Gaye M, and Besnard C
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The title compound, C(7)H(9)N(3)S(2), crystallizes with two unique mol-ecules in the unit cell, both present as thio-semicarbazide tautomers. The mol-ecules differ principally in the dihedral angles between the thio-phene ring planes and the planes through the non-H atoms of the hydrazinecarbothio-amide units, viz. 9.80 (8)° for one mol-ecule and 19.37 (7)° for the other. The hydrazinecarbothio-amide units are reasonably planar, with r.m.s. deviations of 0.001Å for each of the mol-ecules. In the crystal, N-H⋯S hydrogen bonds link like mol-ecules into R(2) (2)(8) inversion dimers. A three-dimensional network structure is generated by additional N-H⋯S hydrogen bonds and weak C-H⋯S contacts between the unique mol-ecules.
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- 2011
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7. Impact of HIV type 1 subtype on drug resistance mutations in Nigerian patients failing first-line therapy.
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Chaplin B, Eisen G, Idoko J, Onwujekwe D, Idigbe E, Adewole I, Gashau W, Meloni S, Sarr AD, Sankalé JL, Ekong E, Murphy RL, and Kanki P
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- Amino Acid Substitution, Anti-HIV Agents therapeutic use, Antiretroviral Therapy, Highly Active methods, Female, Genotype, HIV Protease genetics, HIV Reverse Transcriptase genetics, HIV-1 classification, HIV-1 isolation & purification, Humans, Male, Molecular Sequence Data, Nigeria, RNA, Viral genetics, Sequence Analysis, DNA, Treatment Failure, Anti-HIV Agents pharmacology, Drug Resistance, Viral, HIV Infections drug therapy, HIV Infections virology, HIV-1 drug effects, HIV-1 genetics, Mutation, Missense
- Abstract
A diverse array of non-subtype B HIV-1 viruses circulates in Africa and dominates the global pandemic. It is important to understand how drug resistance mutations in non-B subtypes may develop differently from the patterns described in subtype B. HIV-1 reverse transcriptase and protease sequences from 338 patients with treatment failure to first-line ART regimens were evaluated. Multivariate logistic regression was used to examine the effect of subtype on each mutation controlling for regimen, time on therapy, and total mutations. The distribution of HIV-1 subtypes included CRF02_AG (45.0%), G (37.9%), CRF06_cpx (4.4%), A (3.6%), and other subtypes or recombinant sequences (9.2%). The most common NRTI mutations were M184V (89.1%) and thymidine analog mutations (TAMs). The most common NNRTI mutations were Y181C (49.7%), K103N (36.4%), G190A (26.3%), and A98G (19.5%). Multivariate analysis showed that CRF02_AG was less likely to have the M41L mutation compared to other subtypes [adjusted odds ratio (AOR) = 0.35; p = 0.022]. Subtype A patients showed a 42.5-fold increased risk (AOR = 42.5, p = 0.001) for the L210W mutation. Among NNRTI mutations, subtype G patients had an increased risk for A98G (AOR = 2.40, p = 0.036) and V106I (AOR = 6.15, p = 0.010), whereas subtype CRF02_AG patients had an increased risk for V90I (AOR = 3.16; p = 0.003) and a decreased risk for A98G (AOR = 0.48, p = 0.019). Five RT mutations were found to vary significantly between different non-B West African subtypes. Further study to understand the clinical impact of subtype-specific diversity on drug resistance will be critically important to the continued success of ART scale-up in resource-limited settings.
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- 2011
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8. 4,6-Dimethyl-2-thioxo-1,2-dihydro-pyrimidin-3-ium chloride-thio-urea (1/1).
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Gaye PA, Barry AH, Gaye M, Sarr AD, and Sall AS
- Abstract
In the title compound, C(6)H(9)N(2)S(+)·Cl(-)·CH(4)N(2)S, the 4,6-di-methyl-2-thioxo-1,2-dihydro-pyrimidin-3-ium cation is proton-ated at one of the pyrimidine N atoms. The cations are bridged by the chloride anions through a pair of N-H⋯Cl hydrogen bonds. The amino groups of each thio-urea adduct inter-act with the chloride anions through a pair of N-H⋯Cl hydrogen bonds and the S atom of another thio-urea adduct through a pair of N-H⋯S hydrogen bonds. These inter-actions result in a layered hydrogen-bonded network propagating parallel to the bc plane. Except for two H atoms, all atoms are on special positions.
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- 2009
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9. The level of APOBEC3G (hA3G)-related G-to-A mutations does not correlate with viral load in HIV type 1-infected individuals.
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Ulenga NK, Sarr AD, Hamel D, Sankale JL, Mboup S, and Kanki PJ
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- APOBEC-3G Deaminase, Animals, Female, HIV-1 isolation & purification, Humans, Molecular Sequence Data, Polymorphism, Genetic, Sequence Analysis, DNA, env Gene Products, Human Immunodeficiency Virus genetics, gag Gene Products, Human Immunodeficiency Virus genetics, vif Gene Products, Human Immunodeficiency Virus genetics, Cytidine Deaminase immunology, HIV Infections immunology, HIV Infections virology, HIV-1 genetics, Point Mutation, Viral Load
- Abstract
The APOBEC family of mammalian cytidine deaminases, such as APOBEC3G (hA3G), has been demonstrated to function as a host viral restriction factor against HIV-1. hA3G has been shown to cause extensive G-to-A mutations in the HIV-1 genome, which may play a role in viral restriction. To investigate the role of G-to-A mutations in HIV-1 pathogenesis, we isolated, amplified, and sequenced HIV-1 sequences (vif, gag, and env) from 29 therapy-naive HIV-1-infected individuals. The levels of G-to-A mutations correlated with the expression levels of hA3G in the vif (rho = 0.438, p = 0.041) and the env regions (rho = 0.392, p = 0.038), but not in the gag region (rho = 0.131, p = 0.582). There is no correlation between viral load and the level of G-to-A mutations in the vif (rho = 0.144, p = 0.522), env (rho = 0.168, p = 0.391), or gag regions (rho = -0.254, p = 0.279). Taken together, these findings suggest that the hA3G-induced G-to-A mutations may not be the mechanism by which hA3G restricts or controls viral replication. Thus, hA3G might be restricting viral growth in infected individuals through a mechanism that is independent of the cytidine deaminase activities of hA3G.
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- 2008
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10. Relationship between human immunodeficiency type 1 infection and expression of human APOBEC3G and APOBEC3F.
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Ulenga NK, Sarr AD, Thakore-Meloni S, Sankalé JL, Eisen G, and Kanki PJ
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- APOBEC-3G Deaminase, Adult, Cytidine Deaminase genetics, Cytosine Deaminase genetics, Female, HIV Infections immunology, HIV-1 pathogenicity, Humans, Viral Load, Viremia, Cytidine Deaminase metabolism, Cytosine Deaminase metabolism, HIV Infections metabolism, HIV-1 physiology, RNA, Messenger analysis
- Abstract
Background: Human immunodeficiency virus type 1 (HIV-1)-infected individuals with a high viral set point progress to acquired immunodeficiency syndrome (AIDS) more rapidly than those with a low viral set point. It is not entirely clear which host and viral factors are responsible for the viral set point. Host factors that affect virus replication are likely to influence the viral set point. Human APOBEC proteins have been shown to restrict HIV-1 replication., Methods: This prospective study was conducted to determine the relationship between human APOBEC3G (hA3G) and APOBEC3F (hA3F) levels and the viral set point. Fourteen subjects were classified as having a high viral set point, and 16 were classified as having a low viral set point. We quantified the levels of hA3G and hA3F mRNA in HIV-1-infected, antiretroviral drug-naive individuals before and after infection., Results: We found a significant correlation between the hA3G mRNA level and the viral set point. The expression of hA3G and hA3F increased after infection, and the levels of hA3G and hA3F mRNA were significantly higher after infection in the low viral set point group, compared with the high viral set point group., Conclusions: The results suggest that the level of hA3G expression affects the establishment of the viral set point and may therefore function as a host determinant in the pathogenesis of HIV-1 infection.
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- 2008
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11. Comparison of heterologous neutralizing antibody responses of human immunodeficiency virus type 1 (HIV-1)- and HIV-2-infected Senegalese patients: distinct patterns of breadth and magnitude distinguish HIV-1 and HIV-2 infections.
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Rodriguez SK, Sarr AD, MacNeil A, Thakore-Meloni S, Gueye-Ndiaye A, Traoré I, Dia MC, Mboup S, and Kanki PJ
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- Adult, Cross Reactions, Disease Progression, Female, HIV Infections physiopathology, Humans, Neutralization Tests, Senegal, Viral Load, Viremia, HIV Antibodies immunology, HIV Infections immunology, HIV Infections virology, HIV-1 immunology, HIV-2 immunology
- Abstract
Neutralizing antibody responses against heterologous isolates in human immunodeficiency virus type 1 (HIV-1) and HIV-2 infections were compared, and their relationships with established clinical markers of progression were examined. Neutralizing responses against 7 heterologous primary isolates and 1 laboratory strain were compared between 32 untreated HIV-1-infected subjects and 35 untreated HIV-2-infected subjects using a pseudotyped reporter virus assay. The breadth of the neutralizing response, defined as the proportion of panel viruses positively neutralized by patient plasma, was significantly greater among HIV-2-infected subjects than among HIV-1-infected subjects. Notably, for fully one-third of HIV-2 subjects, all viruses were effectively neutralized in our panel. Magnitudes of responses, defined as reciprocal 50% inhibitory concentration (IC(50)) titers for positive reactions, were significantly greater among HIV-1-infected subjects than among HIV-2-infected subjects. When plasma samples from HIV-1 patients were tested for cross-neutralization of HIV-2 and vice versa, we found that these intertype responses are very rare and their prevalences comparable in both HIV-1 and HIV-2 infection. The significantly higher magnitude of heterologous responses for HIV-1 compared to HIV-2 prompted us to examine associations with viremia, which is known to be significantly higher in HIV-1 infection. Importantly, there was a significant positive correlation between the IC(50) titer and viral load within both the HIV-1 and HIV-2 groups, suggesting heterologous antibodies may be driven by viral replication. We conclude that HIV-2 infection is characterized by a broad, low-magnitude intratype neutralization response, while HIV-1 is characterized by a narrower but higher-magnitude intratype response and that a significant positive association between the IC(50) titer and viremia is common to both HIV-1 and HIV-2 infections.
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- 2007
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12. Direct evidence of lower viral replication rates in vivo in human immunodeficiency virus type 2 (HIV-2) infection than in HIV-1 infection.
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MacNeil A, Sarr AD, Sankalé JL, Meloni ST, Mboup S, and Kanki P
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- CD4 Lymphocyte Count, DNA, Viral analysis, Female, Fluorescent Antibody Technique, Direct, HIV-1 genetics, HIV-2 genetics, Humans, Leukocytes, Mononuclear virology, Plasma virology, Proviruses genetics, RNA, Viral analysis, Senegal, Viral Load, HIV Infections virology, HIV-1 physiology, HIV-2 physiology
- Abstract
Studies have shown that human immunodeficiency virus type 2 (HIV-2) is less pathogenic than HIV-1, with a lower rate of disease progression. Similarly, plasma viral loads are lower in HIV-2 infection, suggesting that HIV-2 replication is restricted in vivo in comparison to that of HIV-1. However, to date, in vivo studies characterizing replication intermediates in the viral life cycle of HIV-2 have been limited. In order to test the hypothesis that HIV-2 has a lower replication rate in vivo than HIV-1 does, we quantified total viral DNA, integrated proviral DNA, cell-associated viral mRNA, and plasma viral loads in peripheral blood samples from groups of therapy-naïve HIV-1-infected (n = 21) and HIV-2-infected (n = 18) individuals from Dakar, Senegal, with CD4(+) T-cell counts of >200/microl. Consistent with our previous findings, total viral DNA loads were similar between HIV-1 and HIV-2 and plasma viral loads were higher among HIV-1-infected individuals. Proportions of DNA in the integrated form were also similar between these viruses. In contrast, levels of viral mRNA were lower in HIV-2 infection. Our study indicates that HIV-2 is able to establish a stable, integrated proviral infection in vivo, but that accumulation of viral mRNA is attenuated in HIV-2 infection relative to that in HIV-1 infection. The differences in viral mRNA are consistent with the differences in plasma viral loads between HIV-1 and HIV-2 and suggest that lower plasma viral loads, and possibly the attenuated pathogenesis of HIV-2, can be explained by lower rates of viral replication in vivo.
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- 2007
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13. Long-term intrapatient viral evolution during HIV-2 infection.
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MacNeil A, Sankale JL, Meloni ST, Sarr AD, Mboup S, and Kanki P
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- Amino Acid Sequence, Female, Gene Products, env genetics, Gene Products, env metabolism, Genetic Variation, HIV Infections epidemiology, HIV-1 genetics, Humans, Phylogeny, Senegal epidemiology, Time Factors, Virus Replication, Evolution, Molecular, HIV Infections virology, HIV-2 genetics, HIV-2 physiology
- Abstract
Background. Disease progression and transmission of human immunodeficiency virus (HIV) type 2 are attenuated, compared with HIV-1, which is consistent with the lower plasma viral loads observed in HIV-2 infection. Although numerous studies have characterized the intrapatient evolution of viral sequences during HIV-1 infection, prospective studies examining intrapatient evolution during HIV-2 infection have been limited.Methods. We examined viral sequence evolution in the C2V3C3 region of the viral env gene in 8 HIV-2-infected individuals from Dakar, Senegal, over the course of approximately 10 years. To compare results with HIV-1 infection, we reanalyzed data from our previous study that prospectively examined intrapatient viral evolution in HIV-1-infected individuals from the same population.Results. HIV-2 sequences from early and late time points were phylogenetically intermixed for all subjects. No distinct trends were observed in terms of increases or decreases in fragment size or the number of N-linked glycosylation sites, and ratios of synonymous substitutions per synonymous site to nonsynonymous substitutions per nonsynonymous site suggested selection to be neutral or negative. In homologous env C2V3 sequences, rates of viral divergence and diversification were slower in individuals infected with HIV-2 than in those infected with HIV-1.Conclusions. Viral evolution occurs slowly in HIV-2 infection, which is consistent with the slow disease progression of HIV-2 and supports the notion that viral evolution may be a relevant correlate for disease progression.
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- 2007
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14. The absence of anti-Tat antibodies is associated with risk of disease progression in HIV-2 infection.
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Rodriguez SK, Sarr AD, Olorunnipa O, Popper SJ, Gueye-Ndiaye A, Traoré I, Dia MC, Mboup S, and Kanki PJ
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- Adult, Disease Progression, Enzyme-Linked Immunosorbent Assay, Female, HIV Antibodies blood, Humans, Immunoglobulin G blood, Immunoglobulin G immunology, Longitudinal Studies, Middle Aged, Risk Factors, Survival Analysis, Time Factors, tat Gene Products, Human Immunodeficiency Virus, Gene Products, tat immunology, HIV Antibodies immunology, HIV Infections immunology, HIV-2 immunology
- Abstract
The Tat protein of human immunodeficiency virus (HIV) is essential for viral replication and has extracellular pathogenic activity. We sought to determine whether the anti-Tat antibody response was predictive of disease progression in 144 HIV type 2 (HIV-2)-infected subjects observed longitudinally between 1985 and 2003. Sixty-eight percent of the subjects tested positive for anti-Tat antibodies, with reactivity notably established early after seroconversion and stably maintained over the course of infection. The risk and rate of progression to advanced HIV-2 AIDS was significantly higher in anti-Tat-negative subjects than in anti-Tat-positive subjects, extending the importance of this prognostic marker for HIV-2 AIDS.
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- 2006
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15. Subtype-specific patterns in HIV Type 1 reverse transcriptase and protease in Oyo State, Nigeria: implications for drug resistance and host response.
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Ojesina AI, Sankalé JL, Odaibo G, Langevin S, Meloni ST, Sarr AD, Olaleye D, and Kanki PJ
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- Amino Acid Sequence, Anti-Retroviral Agents administration & dosage, Genes, pol genetics, HIV Infections drug therapy, HIV-1 classification, Humans, Molecular Sequence Data, Nigeria, Phylogeny, Polymorphism, Genetic drug effects, Species Specificity, Drug Resistance, Viral genetics, HIV Infections virology, HIV Protease genetics, HIV Reverse Transcriptase genetics, HIV-1 enzymology, Polymorphism, Genetic genetics
- Abstract
As the use of antiretroviral therapy becomes more widespread across Africa, it is imperative to characterize baseline molecular variability and subtype-specific peculiarities of drug targets in non-subtype B HIV-1 infection. We sequenced and analyzed 35 reverse transcriptase (RT) and 43 protease (PR) sequences from 50 therapy-naive HIV-1-infected Nigerians. Phylogenetic analyses of RT revealed that the predominant viruses were CRF02_AG (57%), subtype G (26%), and CRF06_cpx (11%). Six of 35 (17%) individuals harbored primary mutations for RT inhibitors, including M41L, V118I, Y188H, P236L, and Y318F, and curiously three of the six were infected with CRF06_cpx. Therefore, CRF06_cpx drug-naive individuals had significantly more drug resistance mutations than the other subtypes (p = 0.011). By combining data on quasisynonymous codon bias with the influence of the differential genetic cost of mutations, we were able to predict some mutations, which are likely to predominate by subtype, under drug pressure. Some subtype-specific polymorphisms occurred within epitopes for HLA B7 and B35 in the RT, and HLA A2 and A*6802 in PR, at positions implicated in immune evasion. Balanced polymorphism was also observed at predicted serine-threonine phosphorylation sites in the RT of subtype G viruses. The subtype-specific codon usage and polymorphisms observed suggest the involvement of differential pathways for drug resistance and host-driven viral evolution in HIV-1 CRF02_AG, subtype G, and CRF06_cpx, compared to subtype B. Subtype-specific responses to HIV therapy may have significant consequences for efforts to provide effective therapy to the populations infected with these HIV-1 subtypes.
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- 2006
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16. Genomic sites of human immunodeficiency virus type 2 (HIV-2) integration: similarities to HIV-1 in vitro and possible differences in vivo.
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MacNeil A, Sankalé JL, Meloni ST, Sarr AD, Mboup S, and Kanki P
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- Base Composition, Binding Sites, Chromosomes, Human genetics, DNA, Viral genetics, Female, Genome, Human, Heterochromatin physiology, Humans, In Vitro Techniques, Leukocytes, Mononuclear virology, Molecular Sequence Data, Virus Replication, HIV-1 genetics, HIV-2 genetics, Virus Integration
- Abstract
Retroviruses have distinct preferences in integration site selection in the host cell genome during in vitro infection, with human immunodeficiency virus type 1 (HIV-1) integration strongly favoring transcriptional units. Additionally, studies with HIV-1 have shown that the genomic site of proviral integration may impact viral replication, with integration in heterochromatin associated with a block in viral transcription. HIV-2 is less pathogenic than HIV-1 and is believed to have a lower replication rate in vivo. Although differences in integration site selection between HIV-2 and HIV-1 could potentially explain the attenuated pathogenicity of HIV-2, no studies have characterized integration site selection by HIV-2. In this study, we mapped 202 HIV-2 integration sites during in vitro infection of peripheral blood mononuclear cells with a primary HIV-2 isolate. In addition, we assayed for in vivo proviral integration within heterochromatin in 21 HIV-1-infected subjects and 23 HIV-2-infected subjects, using an alphoid repeat PCR assay. During in vitro infection, HIV-2 displayed integration site preferences similar to those previously reported for HIV-1. Notably, 82% of HIV-2 integrations mapped to Refseq genes, and integration strongly favored regions of the genome with high gene density and high GC content. Though rare, the proportion of HIV-2 subjects with evidence of proviral integration within heterochromatin in vivo was higher than that of HIV-1-infected subjects. It is therefore possible that integration site selection may play a role in the differences in HIV-1 and HIV-2 in vivo pathogenesis.
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- 2006
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17. Distinct profile of T cell activation in HIV type 2 compared to HIV type 1 infection: differential mechanism for immunoprotection.
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Hanson A, Sarr AD, Shea A, Jones N, Mboup S, Kanki P, and Cao H
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- Female, Gene Products, gag immunology, HIV Infections virology, Humans, Lymphocyte Activation, Prospective Studies, Senegal, Sex Work, Species Specificity, CD4-Positive T-Lymphocytes immunology, CD8-Positive T-Lymphocytes immunology, HIV Infections immunology, HIV-1, HIV-2
- Abstract
The mechanism for the lower rate of disease progression in HIV-2 infection remains undefined. We evaluated T cell activation in a cohort of HIV-infected commercial sex workers in Dakar, Senegal. CD8+ T cell activation was significantly lower in HIV-2- compared to HIV-1-infected volunteers and both groups displayed higher activation levels compared to seronegative individuals. In contrast, CD4+ T cell activation was similar between the HIV-1 and HIV-2 groups and significantly higher compared to the seronegative group. Interestingly, HIV-2-positive volunteers with evidence of Gag-specific CD8+ T cell responses displayed lower CD4+ T cell activation. Our data suggest that the distinct T cell activation profile in HIV-2-positive individuals may reflect on the presence of effective host immune responses in HIV-2 infection.
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- 2005
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18. Viral dynamics of primary HIV-1 infection in Senegal, West Africa.
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Sarr AD, Eisen G, Guèye-Ndiaye A, Mullins C, Traoré I, Dia MC, Sankalé JL, Faye D, Mboup S, and Kanki P
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- Acquired Immunodeficiency Syndrome blood, Acquired Immunodeficiency Syndrome virology, Cohort Studies, Female, HIV Antibodies blood, Humans, Incidence, Phylogeny, Senegal epidemiology, Sex Work, Viral Load, Acquired Immunodeficiency Syndrome epidemiology, HIV-1 classification, HIV-1 isolation & purification
- Abstract
Background: Few studies have addressed primary human immunodeficiency virus (HIV) type 1 infection in sub-Saharan Africa, where the epidemic is of a predominantly heterosexual character and is caused by different subtypes. The present study examines the dynamics of viral replication in subjects infected with various HIV-1 subtypes., Methods: Seven hundred fifty-two HIV-negative Senegalese women at high risk for infection were monitored every 3 months for acute/early HIV infection; 26 infections were identified (23 HIV-1 and 3 HIV-2), with an HIV-1 incidence rate of 3.23 cases/person-years observation. Multiple viral-load measurements were taken for all seroconverters., Results: The mean+/-standard deviation viral load for all subjects during the early stage of infection was 4.13+/-0.66 log10 copies/mL, with an overall decrease of 0.22 log10 copies/mL after the early stage; the viral set point was reached after 12 months of infection. Most subjects had relatively low viral loads during the early stage of infection. HIV-1 CRF02_AG-infected women had a significantly higher mean viral load during the early stage of infection (mean +/- SD, 4.45+/-0.60 log(10) copies/mL) than did non-HIV-1 CRF02_AG-infected women (mean+/-SD, 3.78+/-0.46 log(10) copies/mL) (P=.008). None of the subjects reported symptoms consistent with primary HIV-1 infection., Conclusion: Our findings in Senegalese women differ from what have been described for primary HIV-1 infection. Further investigations of primary infections with non-B subtypes are warranted, to better characterize their differences with primary infections with subtype B.
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- 2005
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19. Associations between MHC class I and susceptibility to HIV-2 disease progression.
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Diouf K, Sarr AD, Eisen G, Popper S, Mboup S, and Kanki P
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- Alleles, Case-Control Studies, Disease Progression, Female, Gene Frequency, Gene Products, gag immunology, HIV Antibodies blood, HIV Antigens immunology, HIV Infections genetics, HIV Infections virology, HLA-B35 Antigen genetics, Humans, Senegal, Sex Work, gag Gene Products, Human Immunodeficiency Virus, Genes, MHC Class I, Genetic Predisposition to Disease, HIV Infections physiopathology, HIV-2 immunology
- Abstract
Objectives: Human immunodeficiency virus type 2 (HIV-2) progression to disease is significantly slower than that of human immunodeficiency virus type 1 (HIV-1). Genetic determinants for susceptibility to disease progression were hypothesized to play a more significant role in this infection compared with HIV-1. We sought to identify common human lymphocyte antigen (HLA) alleles in the Senegalese population and to compare HLA profiles between HIV-2-infected individuals with low and high risk for disease progression., Study Design/methods: We conducted a case-control study investigating possible associations between MHC class I genes and the risk of disease progression in HIV-2-infected individuals. The MHC class I genotype was molecularly defined using polymerase chain reaction with sequence specific primers (PCR-SSP) in 62 female sex workers from the Dakar, Senegal cohort. Lack of antibodies to the HIV-2 antigen p26 has been previously shown to predict disease progression and was used in this study as a surrogate marker. Twenty-one cases were identified lacking antibodies to p26, therefore at a higher risk of disease progression, and were compared with 41 p26 antibody-positive, randomly selected controls., Results: Statistical analysis showed that HLA B35 was significantly associated with lack of p26 antibodies, and higher risk of disease progression ( < 0.05). The same association was found for the self-defined class I haplotypes B35-Cw4 and A23-Cw 7 ( < 0.05). The HLA B 53 allele was associated with slower disease progression; however, this association was not statistically significant. We observed a trend whereby heterozygotes were at lower risk for HIV-2 disease progression, as previously reported in HIV-1 disease., Conclusions: In this West African population, a distinct profile of HLA class I alleles was observed, and many of these appear to influence disease progression in HIV-2 infection.
- Published
- 2002
20. Immunologic and virologic response after tetanus toxoid booster among HIV-1- and HIV-2-infected Senegalese individuals.
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Dieye TN, Sow PS, Simonart T, Guèye-Ndiaye A, Popper SJ, Delforge ML, Dieye A, Sarr AD, Crusiaux A, Van Vooren JP, Devleeschouwer M, Kanki P, Mboup S, Diakhate L, and Farber CM
- Subjects
- Adult, Antibodies, Bacterial blood, Apoptosis, CD4 Lymphocyte Count, Case-Control Studies, HIV Infections virology, Humans, Immunization, Secondary, Immunoglobulin G blood, Leukocytes, Mononuclear pathology, Lymphocyte Activation, Middle Aged, RNA, Viral blood, Senegal, T-Lymphocyte Subsets immunology, HIV Infections immunology, HIV-1, HIV-2, Tetanus Toxoid administration & dosage
- Abstract
Twelve HIV-1-infected, nine HIV-2-infected patients and eight HIV-negative subjects were given a 40IU booster dose of tetanus toxoid (TT). Blood was collected on days 0, 7 and 30 after immunization. Changes in HIV-1 or HIV-2 RNA load were evaluated by nested PCR. TT-IgG antibody levels were quantified by ELISA. CD4 cell counts as well as activation, memory and maturation markers of T lymphocyte subsets were determined by flow cytometry. The induction of apoptosis was investigated using 7-aminoactinomycin D (AAD) and propidium iodide (PI) staining. Proliferative responses to TT and pokeweed mitogen (PWM) were determined by the level of [(3)H] thymidine incorporation. Seven and 30 days after immunization, there was no detectable increase in HIV-1 or HIV-2 plasma load. There were also no changes in CD4 cell counts, CD69, HLA-DR and memory CD45RO or naive CD45RA antigens. Immunization did not increase the spontaneous apoptosis of peripheral blood mononuclear cells (PBMCs), CD4+ and CD8+ T cells subsets neither in controls nor in HIV-infected patients. Similarly, apoptosis induced in vitro by PWM or by the specific TT recall antigen did not vary during the study period. The proliferative response to PWM and to the TT recall antigen was decreased both in HIV-1- and HIV-2-infected patients compared to HIV-negative controls. Immunization significantly increased the TT-IgG levels in healthy controls and in HIV-infected patients. However, the anti-TT-IgG response, as measured by the fold-increase index between days 0 and 30, was significantly higher in healthy controls than in HIV-1- (P=0.036) and HIV-2-infected patients (P=0.003). In conclusion, we found no deleterious immunologic or virologic effect was detected in healthy HIV-1- and HIV-2-infected individuals after antigenic challenge with a TT booster. However, the response to TT vaccination was lower in HIV-1- and in HIV-2-infected individuals than in healthy HIV-negative controls.
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- 2001
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21. Robust HIV type 2 cellular immune response measured by a modified anthrax toxin-based enzyme-linked immunospot assay.
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Sarr AD, Lu Y, Sankalé JL, Eisen G, Popper S, Mboup S, Kanki PJ, and Cao H
- Subjects
- Antigen Presentation immunology, Cells, Cultured, Female, Gene Products, gag immunology, HIV Antigens immunology, HIV Infections virology, HIV-2 genetics, HIV-2 physiology, Humans, Leukocytes, Mononuclear, Monitoring, Immunologic methods, RNA, Viral blood, Viral Load, gag Gene Products, Human Immunodeficiency Virus, Antigens, Bacterial, Bacterial Toxins analysis, HIV Infections immunology, HIV-2 immunology, Immunoenzyme Techniques methods, T-Lymphocytes, Cytotoxic immunology, T-Lymphocytes, Helper-Inducer immunology
- Abstract
Evaluation of immune mechanisms responsible for control of viral replication is critical to understanding HIV-2 attenuated biological characteristics in pathogenesis and transmission. Evaluation of the cellular immune response is often based on labor-intensive techniques that limit the scope of most studies performed. A simple and rapid anthrax toxin-based ELISPOT method to assess HIV-2 cellular immune response was developed. The modified anthrax toxin-based antigen presentation process performed better than a recombinant vaccinia system and the ELISPOT method significantly enhanced the ease and simplicity of the assay. Using this method, a robust HIV-2 cellular immune response directed toward the p26 core protein was exhibited in 21 of 24 (87.5%) infected women, and all 8 seronegative subjects were negative in both assays. Cellular immune responses were associated with low HIV-2 viral load. This simple and rapid modified anthrax toxin-based ELISPOT method allowed us to demonstrate, strong cellular immune responses that may be critical determinants in the HIV-2 attenuated phenotype.
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- 2001
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22. Interaction with human immunodeficiency virus (HIV) type 2 predicts HIV type 1 genotype.
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Sarr AD, Sankalé JL, Hamel DJ, Travers KU, Guèye-Ndiaye A, Essex M, Mboup S, and Kanki PJ
- Subjects
- Amino Acid Motifs genetics, Amino Acid Sequence, Female, Genotype, HIV Infections epidemiology, HIV Infections genetics, HIV-1 physiology, HIV-2 genetics, Humans, Molecular Sequence Data, Phylogeny, Senegal epidemiology, Sequence Alignment, HIV Infections virology, HIV-1 genetics, HIV-2 physiology
- Abstract
In West Africa, India, and certain regions of Europe, both human immunodeficiency viruses types 1 and 2 (HIV-1 and HIV-2) are known to cocirculate. To investigate the HIV-1 subtypes involved in dual HIV-1 and HIV-2 infections, we sequenced the envelope C2-V3 region from 29 dually infected female commercial sex workers from Senegal. The majority of women (23 of 29) were infected by HIV-1 subtype A. Within the HIV-1 subtype A sequences, 14 of 23 (60.8%) clustered with the West African associated A/G recombinant form (IbNG), and 9 of 23 (39.2%) formed a separate cluster distinct from the A/G IbNG. In contrast, in HIV-1 singly infected individuals, non-IbNG subtype A was found in only 13 of 98 (13.3%). Therefore, the lack of protection and/or interaction with HIV-2 was associated with a distinct HIV-1 A genotype. These results suggest differences in the biological properties of HIV-1 genotypes and their in vivo interaction with HIV-2., (Copyright 2000 Academic Press.)
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- 2000
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23. Genetic analysis of HIV type 2 in monotypic and dual HIV infections.
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Sarr AD, Sankalé JL, Guèye-Ndiaye A, Essex M, Mboup S, and Kanki PJ
- Subjects
- Base Sequence, DNA, Viral, Female, HIV Envelope Protein gp120 genetics, HIV Infections complications, HIV-2 classification, Humans, Molecular Sequence Data, Peptide Fragments genetics, Phylogeny, Prospective Studies, Gene Products, env genetics, HIV Infections virology, HIV-2 genetics
- Abstract
A significant level of genetic variation among HIV-1 and HIV-2 has been described. The interaction of specific HIV-2 subtypes with HIV-1 may serve to identify potential biological properties associated with dual infection. To genetically characterize the HIV-2 strains circulating in Senegal and their relationship to coinfection with HIV-1, we sequenced the HIV-2 envelope C2-C3 region of 12 subjects coinfected with HIV-1 and HIV-2 and 9 subjects singly infected with HIV-2. The phylogenetic analysis showed that all subjects were infected with HIV-2 subtype A, confirming its predominance in West Africa. We did not observe specific sequences or genetic clustering based on coinfection status.
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- 2000
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24. Low plasma human immunodeficiency virus type 2 viral load is independent of proviral load: low virus production in vivo.
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Popper SJ, Sarr AD, Guèye-Ndiaye A, Mboup S, Essex ME, and Kanki PJ
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- CD4 Lymphocyte Count, Cohort Studies, Female, Humans, Sex Work, Viral Load, DNA, Viral blood, HIV Infections virology, HIV-2 physiology, Proviruses physiology, RNA, Viral blood
- Abstract
Levels of virus in the plasma are closely related to the pathogenicity of human immunodeficiency virus type 1 (HIV-1). HIV-2 is much less pathogenic than HIV-1, and infection with HIV-2 leads to significantly lower plasma viral load. To identify the source of this difference, we measured both viral RNA and proviral DNA in matched samples from 34 HIV-2-infected individuals. Nearly half had undetectable viral RNA loads (<100 copies/ml), but levels of proviral DNA were relatively high and confirmed that quantities of provirus in HIV-1 and HIV-2 infection were similar. Overall, HIV-2 proviral DNA load did not correlate with viral RNA load, and higher viral RNA load was associated with increased production of plasma virus from the proviral template. These results suggest that low viral load in HIV-2 infection is due to decreased rates of viral production, rather than differences in target cell infectivity.
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- 2000
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25. Lower human immunodeficiency virus (HIV) type 2 viral load reflects the difference in pathogenicity of HIV-1 and HIV-2.
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Popper SJ, Sarr AD, Travers KU, Guèye-Ndiaye A, Mboup S, Essex ME, and Kanki PJ
- Subjects
- Adult, CD4 Lymphocyte Count, Cohort Studies, Female, HIV Infections immunology, HIV Infections virology, HIV Seropositivity immunology, HIV Seropositivity virology, HIV-1 genetics, HIV-1 isolation & purification, HIV-2 genetics, HIV-2 isolation & purification, Humans, Reverse Transcriptase Polymerase Chain Reaction, Senegal, Sex Work, Transcription, Genetic, HIV Infections physiopathology, HIV Seropositivity physiopathology, HIV-1 pathogenicity, HIV-2 pathogenicity, RNA, Viral blood, Viral Load
- Abstract
Human immunodeficiency virus type 2 (HIV-2) is less pathogenic than HIV type 1 (HIV-1), but the mechanisms underlying this difference have not been defined. We developed an internally controlled quantitative reverse transcriptase-polymerase chain reaction to measure HIV-2 viral load and determined levels of plasma virus in a cohort of registered commercial sex workers in Dakar, Senegal. The assay has a lower limit of detection of 100 copies/mL and is linear over 4 logs. HIV-2 viral RNA was detectable in 56% of all samples tested; the median load was 141 copies/mL. Levels of viral RNA in the plasma were inversely related to CD4+ cell counts. HIV-2 and HIV-1 viral loads were compared among the seroincident women in the cohort; the median viral load was 30x lower in the HIV-2-infected women (P<.001, Wilcoxon rank sum test), irrespective of the length of time infected. This suggests that plasma viremia is linked to the differences in the pathogenicity of the 2 viruses.
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- 1999
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26. Relation between HIV-2 proviral load and CD4+ lymphocyte count differs in monotypic and dual HIV infections.
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Sarr AD, Popper S, Thior I, Hamel DJ, Sankalé JL, Siby T, Marlink R, Essex M, Mboup S, and Kanki P
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- CD4 Lymphocyte Count, Female, Humans, HIV Infections immunology, HIV Infections virology, HIV-2 genetics, HIV-2 immunology, Proviruses genetics, Proviruses immunology, Viral Load
- Abstract
Objective: To explore and compare the relations between proviral DNA load and CD4+ lymphocyte counts in both HIV-2 monotypic and HIV dual infection., Study Design/methods: In Dakar, Senegal, where the HIV-1 and HIV-2 epidemics overlap, serum and peripheral blood mononuclear cell (PBMC) DNA samples were collected from registered female sex workers and hospitalized patients. Sera were evaluated for reactivity to antigens of HIV-1 and HIV-2 by immunoblot; dual reactivity was confirmed with recombinant envelope peptides for HIV-1 and HIV-2. These samples were then subjected to HIV-1 and HIV-2 proviral DNA polymerase chain reaction (PCR). To evaluate the HIV-2 cellular proviral DNA loads, a quantitative competitive PCR (QC-PCR) was developed using nested primers to amplify the gag region of HIV-2. This assay used an internal competitor generated by inserting 25 bp in the first-round PCR target sequence. T-lymphocyte subset counts were estimated by flow cytometry for both HIV-2 monotypic and dually infected persons., Results: 35 HIV-2-infected and 33 dually seroreactive samples were evaluated in this study. The CD4+ lymphocyte counts were similar in both groups, with mean values of 449 +/- 390 cells/mm3 for the HIV-2 monotypic infected persons and 476 +/- 308 cells/mm3 among the dually infected persons. However, the median proviral loads differed significantly, with those in the HIV-2 group ranging from 63.2 to 669.8 copies/10(5) CD4+ cells and demonstrating an inverse correlation with CD4+ lymphocyte count. The HIV dually infected persons showed less variation in viral load, ranging from 9.9 to 43.3 copies/10(5) CD4+ cells. Among the HIV dually infected persons, low HIV-2 proviral load was correlated with low CD4+ lymphocyte counts., Conclusions: The HIV-2 proviral loads in HIV dually infected persons were significantly lower than those in HIV-2 monotypically infected individuals (P < .0001), despite comparable CD4+ lymphocyte counts. These results suggest that different HIV-2 proviral dynamics prevail in HIV dual infection.
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- 1999
27. HIV-1 and HIV-2 dual infection: lack of HIV-2 provirus correlates with low CD4+ lymphocyte counts.
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Sarr AD, Hamel DJ, Thior I, Kokkotou E, Sankalé JL, Marlink RG, Coll-Seck EM, Essex ME, Siby T, NDoye I, Mboup S, and Kanki PJ
- Subjects
- Blotting, Southern, CD4 Lymphocyte Count, DNA, Viral blood, Disease Progression, Female, HIV Antibodies blood, HIV Antigens blood, HIV Infections diagnosis, HIV Infections immunology, HIV-1 genetics, HIV-1 immunology, HIV-2 genetics, HIV-2 immunology, Humans, Immunoblotting, Polymerase Chain Reaction, Sensitivity and Specificity, HIV Infections virology, HIV-1 isolation & purification, HIV-2 isolation & purification, Proviruses isolation & purification
- Abstract
Objective: We conducted this study to genetically characterize dual infection in individuals demonstrating a dual serological profile., Methods: All subjects were first evaluated by immunoblot for antibody reactivity to the major viral antigens for HIV-1 and HIV-2. Sera were judged to be dual-seropositive if they reacted with strong and equal intensity with the envelope antigens of both HIV-1 and HIV-2 and were confirmed with type-specific recombinant env peptides. We used nested polymerase chain reaction (PCR) to amplify proviral gag and env sequence from peripheral blood mononuclear cell (PBMC) DNA from HIV-1- and HIV-2-infected individuals. Positive amplification was detected after Southern blot hybridization., Results: Plasmid dilution and mixing showed equivalent sensitivity of HIV-1 and HIV-2 primers that was not altered by heterologous target sequences. The DNA PCR showed 100% sensitivity and specificity for detection of monotypic HIV infection. Serologically defined HIV-dual reactives were evaluated by this assay, with 100% detection in female sex workers (21 out of 21), but only 38.5% detection (five out of 13) in hospitalized patients; all being HIV-1 positive only. The lack of HIV-2 proviral signal was significantly correlated with low CD4+ lymphocyte counts (Pvalue = 0.04)., Conclusion: The results suggest that HIV dual infection may not be a static condition. Levels of HIV-2 may decrease with disease progression or sequester in tissue reservoirs; our results may also suggest that HIV-1 effectively overgrows HIV-2 in the dually exposed host individual.
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- 1998
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28. Sexually transmitted diseases and risk of HIV infection in men attending a sexually transmitted diseases clinic in Dakar, Senegal.
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Thior I, Diouf G, Diaw IK, Sarr AD, Hsieh CC, Ndoye I, Mboup S, Chen L, Essex M, Marlink R, and Kanki P
- Subjects
- Adult, Ambulatory Care Facilities statistics & numerical data, Cross-Sectional Studies, HIV Infections epidemiology, Health Knowledge, Attitudes, Practice, Humans, Male, Multivariate Analysis, Risk Factors, Senegal epidemiology, Sex Work, Sexually Transmitted Diseases epidemiology, Socioeconomic Factors, Surveys and Questionnaires, Urban Health, Urban Health Services statistics & numerical data, HIV Infections etiology, HIV Seroprevalence, Sexually Transmitted Diseases etiology
- Abstract
This cross-sectional study was carried out among male outpatients with symptoms of STDs at the STD reference centre at the Institute of Social Hygiene (IHS), Dakar, Senegal, from March 1989 through May 1991. This study was used to determine the prevalence of STDs and HIV among male patients attending an STD clinic and to identify their socio-demographic characteristics and risk factors. A total of 975 patients were enrolled in the study. The most common syndromes were urethritis (76%) and genital ulcers (22%). Considering single infections, the major STD agents were Neisseria gonorrheae (N.gonorrheae, 30%), Chlamydia trachomatis (C.trachomatis, 15%), Treponema pallidum (T.pallidum, 12%), and Haemophilus ducreyi (H.ducreyi, 7%). HIV prevalence was 2.6 percent (25/975). After multivariate analysis, the risk factors associated with HIV infection were a history of sex with prostitutes (odds ratio [OR] = 8.6, 95% confidence interval [CI] = 2.0-37.8), unprotected sexual contact (OR = 5.6, 95% CI = 1.2-25.0), a history of urethritis (OR = 3.4, 95% CI = 1.3-8.9), current STDs due to H.ducreyi or T.pallidum (OR = 6.1, 95% CI = 2-18.8), and mixed STD infection (OR = 5.3, 95% CI = 1.3-21.8). HIV prevalence was quite low in this population compared to similar studies of STD patients from other sub-Saharan African countries. Neisseria gonorrheae and Chlamydia trachomatis were the leading causes of STDs. A history of risky sexual behaviour, previous STDs, current genital ulcers, and mixed STD infections were associated with HIV infection. Further studies are necessary to determine changes in the relationship of STDs and HIV infection in this population.
- Published
- 1997
29. Accuracy of two enzyme immunoassays and cell culture in the detection of Chlamydia trachomatis in low and high risk populations in Senegal.
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Van Dyck E, Samb N, Sarr AD, Van de Velden L, Moran J, Mboup S, Ndoye I, Lamboray JL, Meheus A, and Piot P
- Subjects
- Adolescent, Adult, Cells, Cultured, Chlamydia trachomatis growth & development, Female, Humans, Immunoenzyme Techniques standards, Lymphogranuloma Venereum diagnosis, Middle Aged, Pregnancy, Pregnancy Complications, Infectious diagnosis, Senegal, Sex Work, Chlamydia trachomatis isolation & purification
- Abstract
Two enzyme immunoassays (EIAs), Chlamydiazyme (CZ; Abbott Laboratories) and Pathfinder (PF; Kallestadt), were compared with a cell culture technique in the detection of cervical Chlamydia trachomatis infection in 670 women in urban settings in Senegal (377 pregnant women and 293 prostitutes). Positive CZ and positive PF specimens were tested a second time using a monoclonal antibody blocking technique. True positive specimens were defined as those positive on culture or positive on EIA with confirmation of the result after blocking. Using this definition, the prevalence of genital chlamydial infection was 14.6% and 14.3% in pregnant women and prostitutes respectively. An important difference between the two populations was that the pregnant women were younger than the prostitutes, which might explain the fact that the prevalence of infection among the pregnant women was as high as that among the prostitutes, although the age-adjusted prevalence was higher among prostitutes than among pregnant women. The chlamydial detection rates of cell culture, CZ and PF were 62% (26/42), 69% (29/42) and 86% (36/42) respectively in prostitutes and 76% (42/55), 40% (22/55) and 53% (29/55) respectively in pregnant women. Agreement between the tests was 89%, 85% and 88% for culture/CZ, culture/PF and CZ/PF respectively. However, when data were adjusted for chance agreement, kappa coefficients were 0.40 for culture/CZ, 0.34 for culture/PF and 0.48 for CZ/PF. These results indicate that the accuracy of the EIAs and cell culture may vary greatly in different populations: both EIAs showed a distinctly higher detection rate than culture in prostitutes and a significantly lower detection rate in pregnant women.(ABSTRACT TRUNCATED AT 250 WORDS)
- Published
- 1992
- Full Text
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