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17 results on '"Sarfo, Edward K."'

1. Cholesterol reduction by immunization with a PCSK9 mimic

Author

Zhang, Baoshan, Chuang, Gwo-Yu, Biju, Andrea, Biner, Daniel, Cheng, Jiaxuan, Wang, Yiran, Bao, Saran, Chao, Cara W., Lei, Haotian, Liu, Tracy, Nazzari, Alexandra F., Yang, Yongping, Zhou, Tongqing, Chen, Steven J., Chen, Xuejun, Kong, Wing-Pui, Ou, Li, Parchment, Danealle K., Sarfo, Edward K., SiMa, HaoMin, Todd, John-Paul, Wang, Shuishu, Woodward, Ruth A., Cheng, Cheng, Rawi, Reda, Mascola, John R., and Kwong, Peter D.

Published
2024
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2. Long trimer-immunization interval and appropriate adjuvant reduce immune responses to the soluble HIV-1-envelope trimer base

Author

Duan, Hongying, Corrigan, Angela R., Cheng, Cheng, Biju, Andrea, Gonelli, Christopher A., Olia, Adam S., Teng, I-Ting, Xu, Kai, O’Dell, Sijy, Narpala, Sandeep, Castro, Mike, Serebryannyy, Leonid, Wang, Jennifer, Parchment, Danealle K., Sarfo, Edward K., van Schooten, Jelle, Todd, John-Paul, Wang, Shuishu, Harris, Darcy R., Geng, Hui, Jafari, Alexander J., Woodward, Ruth A., Doria-Rose, Nicole A., Foulds, Kathryn E., McDermott, Adrian B., van Gils, Marit J., Koup, Richard A., Pierson, Theodore C., Kwong, Peter D., and Mascola, John R.

Published
2024
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3. Cleavage-intermediate Lassa virus trimer elicits neutralizing responses, identifies neutralizing nanobodies, and reveals an apex-situated site-of-vulnerability

Author

Gorman, Jason, Cheung, Crystal Sao-Fong, Duan, Zhijian, Ou, Li, Wang, Maple, Chen, Xuejun, Cheng, Cheng, Biju, Andrea, Sun, Yaping, Wang, Pengfei, Yang, Yongping, Zhang, Baoshan, Boyington, Jeffrey C., Bylund, Tatsiana, Charaf, Sam, Chen, Steven J., Du, Haijuan, Henry, Amy R., Liu, Tracy, Sarfo, Edward K., Schramm, Chaim A., Shen, Chen-Hsiang, Stephens, Tyler, Teng, I-Ting, Todd, John-Paul, Tsybovsky, Yaroslav, Verardi, Raffaello, Wang, Danyi, Wang, Shuishu, Wang, Zhantong, Zheng, Cheng-Yan, Zhou, Tongqing, Douek, Daniel C., Mascola, John R., Ho, David D., Ho, Mitchell, and Kwong, Peter D.

Published
2024
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4. Safety and immunogenicity of an HIV-1 prefusion-stabilized envelope trimer (Trimer 4571) vaccine in healthy adults: A first-in-human open-label, randomized, dose-escalation, phase 1 clinical trial

Author

Houser, Katherine V., Gaudinski, Martin R., Happe, Myra, Narpala, Sandeep, Verardi, Raffaello, Sarfo, Edward K., Corrigan, Angela R., Wu, Richard, Rothwell, Ro Shauna, Novik, Laura, Hendel, Cynthia S., Gordon, Ingelise J., Berkowitz, Nina M., Cartagena, Cora Trelles, Widge, Alicia T., Coates, Emily E., Strom, Larisa, Hickman, Somia, Conan-Cibotti, Michelle, Vazquez, Sandra, Trofymenko, Olga, Plummer, Sarah, Stein, Judy, Case, Christopher L., Nason, Martha, Biju, Andrea, Parchment, Danealle K., Changela, Anita, Cheng, Cheng, Duan, Hongying, Geng, Hui, Teng, I-Ting, Zhou, Tongqing, O'Connell, Sarah, Barry, Chris, Carlton, Kevin, Gall, Jason G., Flach, Britta, Doria-Rose, Nicole A., Graham, Barney S., Koup, Richard A., McDermott, Adrian B., Mascola, John R., Kwong, Peter D., and Ledgerwood, Julie E.

Published
2022
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5. A multiclade env–gag VLP mRNA vaccine elicits tier-2 HIV-1-neutralizing antibodies and reduces the risk of heterologous SHIV infection in macaques

Author

Zhang, Peng, Narayanan, Elisabeth, Liu, Qingbo, Tsybovsky, Yaroslav, Boswell, Kristin, Ding, Shilei, Hu, Zonghui, Follmann, Dean, Lin, Yin, Miao, Huiyi, Schmeisser, Hana, Rogers, Denise, Falcone, Samantha, Elbashir, Sayda M., Presnyak, Vladimir, Bahl, Kapil, Prabhakaran, Madhu, Chen, Xuejun, Sarfo, Edward K., Ambrozak, David R., Gautam, Rajeev, Martin, Malcom A., Swerczek, Joanna, Herbert, Richard, Weiss, Deborah, Misamore, Johnathan, Ciaramella, Giuseppe, Himansu, Sunny, Stewart-Jones, Guillaume, McDermott, Adrian, Koup, Richard A., Mascola, John R., Finzi, Andrés, Carfi, Andrea, Fauci, Anthony S., and Lusso, Paolo

Published
2021
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7. Preclinical Development of a Fusion Peptide Conjugate as an HIV Vaccine Immunogen

Author

Ou, Li, Kong, Wing-Pui, Chuang, Gwo-Yu, Ghosh, Mridul, Gulla, Krishana, O’Dell, Sijy, Varriale, Joseph, Barefoot, Nathan, Changela, Anita, Chao, Cara W., Cheng, Cheng, Druz, Aliaksandr, Kong, Rui, McKee, Krisha, Rawi, Reda, Sarfo, Edward K., Schön, Arne, Shaddeau, Andrew, Tsybovsky, Yaroslav, Verardi, Raffaello, Wang, Shuishu, Wanninger, Timothy G., Xu, Kai, Yang, Gengcheng J., Zhang, Baoshan, Zhang, Yaqiu, Zhou, Tongqing, Arnold, Frank J., Doria-Rose, Nicole A., Lei, Q. Paula, Ryan, Edward T., Vann, Willie F., Mascola, John R., and Kwong, Peter D.

Published
2020
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9. Diverse Murine Vaccinations Reveal Distinct Antibody Classes to Target Fusion Peptide and Variation in Peptide Length to Improve HIV Neutralization

Author

Sastry, Mallika, primary, Changela, Anita, additional, Gorman, Jason, additional, Xu, Kai, additional, Chuang, Gwo-Yu, additional, Shen, Chen-Hsiang, additional, Cheng, Cheng, additional, Geng, Hui, additional, O'Dell, Sijy, additional, Ou, Li, additional, Rawi, Reda, additional, Reveiz, Mateo, additional, Stewart-Jones, Guillaume B. E., additional, Wang, Shuishu, additional, Zhang, Baoshan, additional, Zhou, Tongqing, additional, Biju, Andrea, additional, Chambers, Michael, additional, Chen, Xuejun, additional, Corrigan, Angela R., additional, Lin, Bob C., additional, Louder, Mark K., additional, McKee, Krisha, additional, Nazzari, Alexandra F., additional, Olia, Adam S., additional, Parchment, Danealle K., additional, Sarfo, Edward K., additional, Stephens, Tyler, additional, Stuckey, Jonathan, additional, Tsybovsky, Yaroslav, additional, Verardi, Raffaello, additional, Wang, Yiran, additional, Zheng, Cheng-Yan, additional, Chen, Yuling, additional, Doria-Rose, Nicole A., additional, McDermott, Adrian B., additional, Mascola, John R., additional, and Kwong, Peter D., additional

Published
2023
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10. Assessment of Crosslinkers between Peptide Antigen and Carrier Protein for Fusion Peptide-Directed Vaccines against HIV-1

Author

Ou, Li, primary, Gulla, Krishana, additional, Biju, Andrea, additional, Biner, Daniel W., additional, Bylund, Tatsiana, additional, Changela, Anita, additional, Chen, Steven J., additional, Zheng, Cheng-Yan, additional, Cibelli, Nicole, additional, Corrigan, Angela R., additional, Duan, Hongying, additional, Gonelli, Christopher A., additional, Kong, Wing-Pui, additional, Cheng, Cheng, additional, O’Dell, Sijy, additional, Sarfo, Edward K., additional, Shaddeau, Andrew, additional, Wang, Shuishu, additional, Vinitsky, Alison, additional, Yang, Yanhong, additional, Zhang, Baoshan, additional, Zhang, Yaqiu, additional, Koup, Richard A., additional, Doria-Rose, Nicole A., additional, Gall, Jason G., additional, Mascola, John R., additional, and Kwong, Peter D., additional

Published
2022
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11. Immune Monitoring Reveals Fusion Peptide Priming to Imprint Cross-Clade HIV-Neutralizing Responses with a Characteristic Early B Cell Signature

Author

Cheng, Cheng, primary, Duan, Hongying, additional, Xu, Kai, additional, Chuang, Gwo-Yu, additional, Corrigan, Angela R., additional, Geng, Hui, additional, O’Dell, Sijy, additional, Ou, Li, additional, Chambers, Michael, additional, Changela, Anita, additional, Chen, Xuejun, additional, Foulds, Kathryn E., additional, Sarfo, Edward K., additional, Jafari, Alexander J., additional, Hill, Kurt R., additional, Kong, Rui, additional, Liu, Kevin, additional, Todd, John P., additional, Tsybovsky, Yaroslav, additional, Verardi, Raffaello, additional, Wang, Shuishu, additional, Wang, Yiran, additional, Wu, Winston, additional, Zhou, Tongqing, additional, Arnold, Frank J., additional, Doria-Rose, Nicole A., additional, Koup, Richard A., additional, McDermott, Adrian B., additional, Scorpio, Diana G., additional, Worobey, Michael, additional, Shapiro, Lawrence, additional, Mascola, John R., additional, and Kwong, Peter D., additional

Published
2020
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12. Altered synaptic ultrastructure in the prefrontal cortex of Shank3-deficient rats

Author

Jacot-Descombes, Sarah, primary, Keshav, Neha U, additional, Dickstein, Dara L., additional, Wicinski, Bridget, additional, Janssen, William G. M., additional, Hiester, Liam L., additional, Sarfo, Edward K., additional, Warda, Tahia, additional, Fam, Matthew M., additional, Harony-Nicolas, Hala, additional, Buxbaum, Joseph D., additional, Hof, Patrick R., additional, and Varghese, Merina, additional

Published
2020
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13. Development of a 3Mut-Apex-Stabilized Envelope Trimer That Expands HIV-1 Neutralization Breadth When Used To Boost Fusion Peptide-Directed Vaccine-Elicited Responses

Author

Chuang, Gwo-Yu, primary, Lai, Yen-Ting, additional, Boyington, Jeffrey C., additional, Cheng, Cheng, additional, Geng, Hui, additional, Narpala, Sandeep, additional, Rawi, Reda, additional, Schmidt, Stephen D., additional, Tsybovsky, Yaroslav, additional, Verardi, Raffaello, additional, Xu, Kai, additional, Yang, Yongping, additional, Zhang, Baoshan, additional, Chambers, Michael, additional, Changela, Anita, additional, Corrigan, Angela R., additional, Kong, Rui, additional, Olia, Adam S., additional, Ou, Li, additional, Sarfo, Edward K., additional, Wang, Shuishu, additional, Wu, Winston, additional, Doria-Rose, Nicole A., additional, McDermott, Adrian B., additional, Mascola, John R., additional, and Kwong, Peter D., additional

Published
2020
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14. A multiclade env–gagVLP mRNA vaccine elicits tier-2 HIV-1-neutralizing antibodies and reduces the risk of heterologous SHIV infection in macaques

Author

Zhang, Peng, Narayanan, Elisabeth, Liu, Qingbo, Tsybovsky, Yaroslav, Boswell, Kristin, Ding, Shilei, Hu, Zonghui, Follmann, Dean, Lin, Yin, Miao, Huiyi, Schmeisser, Hana, Rogers, Denise, Falcone, Samantha, Elbashir, Sayda M., Presnyak, Vladimir, Bahl, Kapil, Prabhakaran, Madhu, Chen, Xuejun, Sarfo, Edward K., Ambrozak, David R., Gautam, Rajeev, Martin, Malcom A., Swerczek, Joanna, Herbert, Richard, Weiss, Deborah, Misamore, Johnathan, Ciaramella, Giuseppe, Himansu, Sunny, Stewart-Jones, Guillaume, McDermott, Adrian, Koup, Richard A., Mascola, John R., Finzi, Andrés, Carfi, Andrea, Fauci, Anthony S., and Lusso, Paolo

Abstract

The development of a protective vaccine remains a top priority for the control of the HIV/AIDS pandemic. Here, we show that a messenger RNA (mRNA) vaccine co-expressing membrane-anchored HIV-1 envelope (Env) and simian immunodeficiency virus (SIV) Gag proteins to generate virus-like particles (VLPs) induces antibodies capable of broad neutralization and reduces the risk of infection in rhesus macaques. In mice, immunization with co-formulated envand gagmRNAs was superior to envmRNA alone in inducing neutralizing antibodies. Macaques were primed with a transmitted-founder clade-B envmRNA lacking the N276 glycan, followed by multiple booster immunizations with glycan-repaired autologous and subsequently bivalent heterologous envs(clades A and C). This regimen was highly immunogenic and elicited neutralizing antibodies against the most prevalent (tier-2) HIV-1 strains accompanied by robust anti-Env CD4+T cell responses. Vaccinated animals had a 79% per-exposure risk reduction upon repeated low-dose mucosal challenges with heterologous tier-2 simian–human immunodeficiency virus (SHIV AD8). Thus, the multiclade env–gagVLP mRNA platform represents a promising approach for the development of an HIV-1 vaccine.

Published
2021
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15. Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization

Author

Kong, Rui, primary, Duan, Hongying, additional, Sheng, Zizhang, additional, Xu, Kai, additional, Acharya, Priyamvada, additional, Chen, Xuejun, additional, Cheng, Cheng, additional, Dingens, Adam S., additional, Gorman, Jason, additional, Sastry, Mallika, additional, Shen, Chen-Hsiang, additional, Zhang, Baoshan, additional, Zhou, Tongqing, additional, Chuang, Gwo-Yu, additional, Chao, Cara W., additional, Gu, Ying, additional, Jafari, Alexander J., additional, Louder, Mark K., additional, O’Dell, Sijy, additional, Rowshan, Ariana P., additional, Viox, Elise G., additional, Wang, Yiran, additional, Choi, Chang W., additional, Corcoran, Martin M., additional, Corrigan, Angela R., additional, Dandey, Venkata P., additional, Eng, Edward T., additional, Geng, Hui, additional, Foulds, Kathryn E., additional, Guo, Yicheng, additional, Kwon, Young D., additional, Lin, Bob, additional, Liu, Kevin, additional, Mason, Rosemarie D., additional, Nason, Martha C., additional, Ohr, Tiffany Y., additional, Ou, Li, additional, Rawi, Reda, additional, Sarfo, Edward K., additional, Schön, Arne, additional, Todd, John P., additional, Wang, Shuishu, additional, Wei, Hui, additional, Wu, Winston, additional, Mullikin, James C., additional, Bailer, Robert T., additional, Doria-Rose, Nicole A., additional, Karlsson Hedestam, Gunilla B., additional, Scorpio, Diana G., additional, Overbaugh, Julie, additional, Bloom, Jesse D., additional, Carragher, Bridget, additional, Potter, Clinton S., additional, Shapiro, Lawrence, additional, Kwong, Peter D., additional, and Mascola, John R., additional

Published
2019
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16. Consistent elicitation of cross-clade HIV-neutralizing responses achieved in guinea pigs after fusion peptide priming by repetitive envelope trimer boosting

Author

Cheng, Cheng, primary, Xu, Kai, additional, Kong, Rui, additional, Chuang, Gwo-Yu, additional, Corrigan, Angela R., additional, Geng, Hui, additional, Hill, Kurt R., additional, Jafari, Alexander J., additional, O’Dell, Sijy, additional, Ou, Li, additional, Rawi, Reda, additional, Rowshan, Ariana P., additional, Sarfo, Edward K., additional, Sastry, Mallika, additional, Saunders, Kevin O., additional, Schmidt, Stephen D., additional, Wang, Shuishu, additional, Wu, Winston, additional, Zhang, Baoshan, additional, Doria-Rose, Nicole A., additional, Haynes, Barton F., additional, Scorpio, Diana G., additional, Shapiro, Lawrence, additional, Mascola, John R., additional, and Kwong, Peter D., additional

Published
2019
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17. Development of a 3Mut-Apex-Stabilized Envelope Trimer That Expands HIV-1 Neutralization Breadth When Used To Boost Fusion Peptide-Directed Vaccine-Elicited Responses.

Author

Gwo-Yu Chuang, Yen-Ting Lai, Boyington, Jeffrey C., Cheng Cheng, Hui Geng, Narpala, Sandeep, Rawi, Reda, Schmidt, Stephen D., Tsybovsky, Yaroslav, Verardi, Raffaello, Kai Xu, Yongping Yang, Baoshan Zhang, Chambers, Michael, Changela, Anita, Corrigan, Angela R., Rui Kong, Olia, Adam S., Li Ou, and Sarfo, Edward K.

Subjects
*N-terminal residues, *DIHEDRAL angles, *VACCINE effectiveness, *GUINEA pigs, *VACCINE trials
Abstract

HIV-1 envelope (Env) trimers, stabilized in a prefusion-closed conformation, can elicit humoral responses capable of neutralizing HIV-1 strains closely matched in sequence to the immunizing strain. One strategy to increase elicited neutralization breadth involves vaccine priming of immune responses against a target site of vulnerability, followed by vaccine boosting of these responses with prefusion-closed Env trimers. This strategy has succeeded at the fusion peptide (FP) site of vulnerability in eliciting cross-clade neutralizing responses in standard vaccine-test animals. However, the breadth and potency of the elicited responses have been less than optimal. Here, we identify three mutations (3mut), Met302, Leu320, and Pro329, that stabilize the apex of the Env trimer in a prefusion-closed conformation and show antigenically, structurally, and immunogenically that combining 3mut with other approaches (e.g., repair and stabilize and glycine-helix breaking) yields well-behaved clade C-Env trimers capable of boosting the breadth of FPdirected responses. Crystal structures of these trimers confirmed prefusion-closed apexes stabilized by hydrophobic patches contributed by Met302 and Leu320, with Pro329 assuming canonically restricted dihedral angles. We substituted the N-terminal eight residues of FP (FP8, residues 512 to 519) of these trimers with the second most prevalent FP8 sequence (FP8v2, AVGLGAVF) and observed a 3mutstabilized consensus clade C-Env trimer with FP8v2 to boost the breadth elicited in guinea pigs of FP-directed responses induced by immunogens containing the most prevalent FP8 sequence (FP8v1, AVGIGAVF). Overall, 3mut can stabilize the Env trimer apex, and the resultant apex-stabilized Env trimers can be used to expand the neutralization breadth elicited against the FP site of vulnerability. IMPORTANCE A major hurdle to the development of an effective HIV-1 vaccine is the elicitation of serum responses capable of neutralizing circulating strains of HIV, which are extraordinarily diverse in sequence and often highly neutralization resistant. Recently, we showed how sera with 20 to 30% neutralization breadth could, nevertheless, be elicited in standard vaccine test animals by priming with the most prevalent N-terminal 8 residues of the HIV-1 fusion peptide (FP8), followed by boosting with a stabilized BG505-envelope (Env) trimer. Here, we show that subsequent boosting with a 3mut-apex-stabilized consensus C-Env trimer, modified to have the second most prevalent FP8 sequence, elicits higher neutralization breadth than that induced by continued boosting with the stabilized BG505-Env trimer. With increased neutralizing breadth elicited by boosting with a heterologous trimer containing the second most prevalent FP8 sequence, the fusion peptide-directed immune-focusing approach moves a step closer toward realizing an effective HIV-1 vaccine regimen. [ABSTRACT FROM AUTHOR]

Published
2020
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