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CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-associated proteins) has undergone marked advancements since its discovery as an adaptive immune system in bacteria and archaea, emerged as a potent gene-editing tool after the successful engineering of its synthetic guide RNA (sgRNA) toward the targeting of specific DNA sequences with high accuracy. Besides its DNA editing ability, further-developed Cas variants can also edit the epigenome, rendering the CRISPR-Cas system a versatile tool for genome and epigenome manipulation and a pioneering force in precision medicine. This review explores the latest advancements in CRISPR-Cas technology and its therapeutic and biomedical applications, highlighting its transformative impact on precision medicine. Moreover, the current status of CRISPR therapeutics in clinical trials is discussed. Finally, we address the persisting challenges and prospects of CRISPR-Cas technology., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Azeez, Hamad, Hamad, Shekha and Bergsten.)

The cytokine storm is a complication related to SARS-CoV-2 infection worldwide. This study aimed to address the level of three cytokines which were interleukin-1-beta (IL-1β), interleukin-6 (IL-6), and tumour necrosis factor-alpha (TNF-α), with different blood parameters to the formation of cytokine storm or any complication among COVID-19 patients., Method and Material: A total of 104 serum samples were included for this purpose, and they were divided into three categories the healthy control group ( n =30), mild COVID-19 patients ( n =23), and severe cases of COVID-19 patients ( n =51). The cytokine concentration was measured by enzyme-linked immunosorbent assays (ELISA). Serum ferritin, C-reactive protein (CRP) levels, and erythrocyte sedimentation rate were also evaluated and compared with the concentration of the proinflammatory cytokines., Result: The data analysis showed a significant relationship between the serum IL-6 level with serum ferritin and CRP and the progression to the severity of SARS-CoV-2 infection. The IL-6 level was increased in mild COVID-19 patients and was significantly elevated in severe COVID-19 patients. Patients in the severe group had significantly higher serum ferritin, CRP, and erythrocyte sedimentation rate levels than those in the mild and healthy groups. The IL-1β and TNF-α were not significantly different in the groups compared with the healthy control group., Conclusion: This study revealed that the proinflammatory cytokines and biochemical laboratory tests are promising biomarkers for detecting the severity of COVID-19 cases., Competing Interests: The authors declare no competing interests, (Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc.)

Problem Statement: Grinding restorations, such as veneers, with rotary instruments, is the conventional removal approach. It may be accompanied by micro-fractures that affect the adjacent healthy dental structures. Differentiation of the veneer from the dental structure, as well as the resin cement, is not a highly selective procedure when rotary instruments are used. Moreover, the rotary instruments may lead to scratches and overheating of the enamel. Patient discomfort is another disadvantage, due to the noise of the drill., Purpose: The purpose of this in vitro study was to examine the effectiveness of a 2790 nm Er,Cr:YSGG laser to debond lithium disilicate laminate, utilizing two distinct pulse durations and various exposure times. The shear bond strength, intrapulpal temperature, and adhesive remaining index were evaluated., Methods and Materials: This study included three groups of 75 extracted permanent mandibular incisors: G1, G2 (laser-treated groups were classified according to the pulse duration) and C (control group). Twenty five samples were regarded for each group. Both test groups were irradiated with a 3 W output power of Er,Cr:YSGG laser, for a variety of time intervals (20 s, 30 s, 40 s, 50 s, and 60 s). Five samples were tested for each time interval. During irradiation, the temperature in the pulp chamber was monitored using a K-type thermocouple connected to a digital multilogger thermometer that was introduced into the prepared sample pulp chamber. Subsequently, the shear bond strength was measured for G1 and G2, in addition to the control group (no irradiation). The adhesive remaining index was examined microscopically. The area was measured and analysed, and then, transformed into scores for comparisons. Finally, One untreated sample and two other samples of the highest power value from laser-treated groups were examined for their surface morphology by scanning electron microscope (SEM)., Results: The debonding protocols were safe relative to the intrapulpal protocol. The temperature rise after an exposure time of 50 s and 60 s was significantly different from an exposure time of 20 s, 30 s, and 40 s, in both groups (p < 0.05). Both G1 and G2 significantly outperformed the control group in shear bond strength. There was no significant difference between G1 and G2 at any of the tested exposure times (p > 0.05). Nevertheless, the 60 s exposure time showed the lowest shear bond strength., Conclusion: Regarding intralpulpal temperature, both modes can be safely used to remove laminate veneers. In sum, an exposure time of 50 s and a pulse duration of 60 μs demonstrated superior results for SBS, adhesive remaining index, and temperature values., Clinical Implication: Lithium disilicate laminate veneers may be removed quickly, safely, and comfortably. Laser-assisted debonding of porcelain laminate veneer is recommended and does not cause any damage to the veneer or enamel surface., Competing Interests: The authors declare no conflict of interest., (© 2023 The Authors.)

Listeria monocytogenes has tropism towards two immunologically "privileged" sites, the fetoplacental unit in pregnant women and the central nervous system (neurolisteriosis) in immunocompromised individuals. We report a case of neurolisteriosis in a previously asymptomatic pregnant woman from rural West Bengal, India, who presented with a subacute onset febrile illness with features of rhombencephalitis and a predominantly midline-cerebellopathy (slow and dysmetric saccades, florid downbeat nystagmus, horizontal nystagmus, and ataxia). With timely detection and the institution of prolonged intravenous antibiotic therapy, both the mother and the fetus were saved uneventfully., Competing Interests: No potential conflict of interest was reported by the authors., (© 2023 Taylor & Francis Group, LLC.)

Snakebite is a life-threatening and often-neglected public health hazard with high chronic disability and mortality, mainly faced by rural communities in the tropics/subtropics. Stroke and neuromuscular paralysis are the most severe neurological complications. However, cerebral venous sinus thrombosis has rarely been reported among cerebrovascular complications. We report a previously healthy middle-aged Indian woman who developed cerebral venous sinus thrombosis preceded by features of disseminated bleeding diathesis following Russell's viper bite. The cerebral venous sinus thrombosis was diagnosed by magnetic resonance imaging. The patient responded well to treatment with antivenom and subcutaneous enoxaparin and had no demonstrable neurological deficits at three months of follow-up., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Background: Snakebite is a preventable yet often-neglected public health hazard with high chronic disability and mortality, mainly faced by rural communities in the tropics/subtropics. Endocrinological disorders following snakebite (especially Russell's viper in India) are notably underrecognized and can lead to remarkable morbidity, poor quality of life, and cardiovascular mortality. Anterior pituitary insufficiency has been the most common ailment following Russell's viper envenomation amid those endocrinological dysfunctions. On the contrary, the posterior pituitary and nearby hypothalamus mostly remain unharmed, so central diabetes insipidus is extremely rare following a viperid snakebite envenomation., Case Presentation: The authors present a patient developing panhypopituitarism with evident spontaneous central diabetes insipidus 29 years after Russell's viper envenomation. Relevant investigations ruled out other possible etiologies, and he responded well to hormonal replacement therapy., Conclusions: Panhypopituitarism with concurrent central diabetes insipidus may occur following snakebite (especially in Russell's viper envenomation). Early recognition and proper management of these complications are quintessential to preventing further misdiagnosis, under-recognition, morbidity, impaired quality of life, and mortality., Competing Interests: Competing interests: The authors declare no competing interests.

Aim: The aim of the in vitro study was to comparatively evaluate the bond strength of CAD/CAM monolithic zirconia copings using three luting agents and two different finish lines., Materials and Methods: For the evaluation of bond strength, 60 extracted human maxillary first premolars were prepared to receive CAD/CAM monolithic zirconia copings. They were randomly divided into six groups of 10 samples each to receive CAD/CAM monolithic zirconia copings to bond with three different luting agents on two different finish lines. The luting agents used were RelyX U200, RMGIC, and Maxcem Elite with finish lines which were incorporated were 90° shoulder and radial shoulder finish lines. The tensile bond strength for all 60 samples was tested using the universal testing machine., Results: Between and within the group differences in tensile bond strength were analyzed using one-way ANOVA test of significance with Tukey's post-hoc analysis. The statistical analysis revealed that the CAD/CAM zirconia copings used a combination of 90° shoulder and RelyX U200 as the luting agent (Group II) which offered the maximum bond strength., Conclusion: Within the constraints of this study, it could be concluded that CAD/CAM monolithic zirconia copings luted with RelyX U200 cement on a 90° shoulder finish line is the preferred method, taking the bond strength into consideration., Clinical Significance: The results of this study can be extrapolated into a clinical scenario to help the clinician decide the most apt combination of finish line design and the luting agent to achieve superior bond strengths in CAD/CAM zirconia crowns and fixed dental prosthesis.

Scrub typhus, an acute febrile infectious disease prevalent in the "Tsutsugamushi Triangle", is a mite-born rickettsial zoonosis, caused by Orientia tsutsugamushi . Although the clinical presentation is protean, it rarely causes abducens nerve palsy. We report a 14-year-old previously healthy Indian girl who presented with a recent onset right abducens nerve palsy and headache, but without fever and without the classic dermatological manifestation ("eschar") of the disease. After exclusion of common infectious, autoimmune, and neoplastic causes, she was finally diagnosed with scrub typhus associated with an abducens nerve palsy, which responded to doxycycline therapy., (© 2021 Taylor & Francis Group, LLC.)

Lack of appropriate donors, non-utilization of high throughput phenotyping and genotyping platforms with high genotype × environment interaction restrained identification of robust QTLs for grain protein content (GPC) in rice. In the present investigation a  BC 3 F 4 mapping population was developed using grain protein donor, ARC10075 and high-yielding cultivar Naveen and 190 lines were genotyped using 40 K Affimetrix custom SNP array with the objective to identify stable QTLs for protein content. Three of the identified QTLs, one for GPC (qGPC1.1) and the other two for single grain protein content (qSGPC2.1, qSGPC7.1) were stable over the environments explaining  13%, 14% and 7.8% of the phenotypic variances, respectively. Stability and repeatability of these additive QTLs were supported by the synergistic additive effects of multi-environmental-QTLs. One epistatic-QTL, independent of  the  main effect QTL was detected over the environment for SGPC. A few functional genes governing seed storage protein were hypothesised inside these identified QTLs. The qGPC1.1 was validated by NIR Spectroscopy-based high throughput phenotyping in BC 3 F 5 population. Higher glutelin content was estimated in high-protein lines with the introgression of qGPC1.1 in telomeric region of short arm of chromosome 1. This was supported by the postulation of probable candidate gene inside this QTL region encoding glutelin family proteins.