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One of the challenges that European agriculture will have to face will be to adapt conventional agriculture to procedures of the new European agricultural policies. In this way, organic farming will have more importance in the coming years. One of the most important crops worldwide is grapevine. The main objective of this research work focuses on the physiological, agronomic, and enological characterization of Pedro Ximénez with the purpose of knowing the viability of the organic cultivation of this cultivar in a warm climate zone. Two experimental plots were selected with two different types of management, organic and conventional. In both plots, photosynthetic capacity (AN), stomatal conductance (gs), and intrinsic water-use efficiency (WUEi) were measured, and physicochemical composition of grape must was analyzed during ripening. In addition, bunch and pruning weight were measured as agronomic parameters. Physiological results were not significantly different between management at a general level in gs and WUEi, being possible to identify a difference in AN just before the harvest. At the level of fruit ripening, significant differences were found between the two managements. At harvest, grape must had a higher sugar concentration, amine nitrogen (α-NH2), and yeast assimilable nitrogen (YAN) in the organic management than in conventional, where higher values of pH and l-malic acid were observed. Taking into account the preliminary results obtained it could be concluded the viability of the organic management for Pedro Ximénez crop in a warm climate zone.

The climate change is already affecting many agricultural systems and human environments, and the implementation of adaptation strategies, especially those related to irrigated agriculture in semiarid regions, is urgent. In this regard, deep knowledge about the effects that irrigation has on the food quality parameters will allow us to estimate the potential benefits of deficit irrigation (DI) strategies. This work presents the effects on the quality parameters of three almond cultivars (Marta, Guara, and Lauranne) subjected to three irrigation doses: (i) full-irrigated treatment (FI) at 100% crop evapotranspiration (ETC), (ii) an overirrigated treatment at 150% ETC (150% ETC), and (iii) regulated deficit irrigation (RDI65) treatment, in which irrigation was done as in FI, expect during the kernel-filling period when this treatment received 65% ETC. According to experimental findings, the cultivar most sensitive to water stress was Marta, having the most significant improvements for RDI65. In general, the effects of the irrigation dose on the morphological and physicochemical parameters were not huge but some improvements were observed in key parameters such as the color and contents of specific sugars, organic acids, and unsaturated fatty acids. Thus, it can be concluded that the irrigation dose did not drastically affect the fruit almond quality, although it is possible to improve several key parameters when a moderate RDI strategy is applied.

In recent years, the area dedicated to modern irrigated almond plantations has increased significantly in Spain. However, the legal irrigation allocations are lower than the maximum water requirements of the crop in most cases. Therefore, almond growers are forced to implement regulated deficit irrigation strategies on their farms, applying water stress in certain resistant phenological periods and avoiding it in sensitive periods. Given the need to monitor the water status of the crop, especially in the most sensitive periods to water stress, the objective of this work was to evaluate the sensitivity of two UAV-based crop water status indicators to detect early water stress conditions in four almond cultivars. The field trial was conducted during 2020 in an experimental almond orchard, where two irrigation strategies were established: full irrigation (FI), which received 100% of irrigation requirements (IR), and regulated deficit irrigation (RDI), which received 70% of IR during the whole irrigation period except during the kernel-filling stage when received 40% IR. The UAV flights were performed on four selected dates of the irrigation season. The Crop Water Status Index (CWSI) and the Normalized Difference Vegetation Index (NDVI) were derived from thermal and multispectral images, respectively, and compared to classical water status indicators, i.e., stem water potential (Ψstem), stomatal conductance (gs), and photosynthetic rate (AN). Of the four flights performed, three corresponded to mild water stress conditions and a single flight was performed under moderate water stress conditions. Under mild water stress, CWSI was not able to capture the differences between FI and RDI trees that were observed with Ψstem. Under moderate stress conditions, CWSI was sensitive to the water deficit reached in the trees and showed significant differences among both irrigation treatments. No differences were observed in the CWSI and NVDI response to water stress among cultivars. Although NDVI and CWSI were sensitive to water stress, the low signal intensity observed in NDVI makes this index less robust than CWSI to monitor crop water stress. It can be concluded that UAV-based CWSI measurements are reliable to monitor almond water status, although for early (mild) levels of water stress, Ψstem seems to be the preferred option.

Almond (Prunus dulcis Mill.) has been traditionally associated to marginal areas in Andalusia, being cultivated under rainfed agriculture conditions. In the last few years, it has been progressively introduced in many irrigated areas of the Guadalquivir river basin which has promoted a growing interest about irrigation water management for this crop i.e. the potential irrigation requirements, or the best irrigation strategy under sub-optimal conditions (aiming at obtaining balanced nut yields, saving water without affecting yield). This work examines the response of three almond cultivars (cvs. Guara, Marta and Lauranne) subjected to different irrigation regimes in a semi-arid Mediterranean environment (SW Spain). The trial was conducted over two seasons (2017–2018), in a commercial orchard with three irrigation regimes: i) a full-irrigation treatment (FI), which received 100% of crop evapotranspiration (ETC) as a control; ii) an over irrigated treatment (150-ETC), which received 150% of ETC during the whole irrigation period; and iii) a regulated deficit irrigation treatment (RDI65) which was irrigated at 100% ETC during the whole irrigation season, except during the kernel-filling period, when it received the 65% of ETC. In order to assess the crop water status, the leaf water potential (Ψleaf) and the stomatal conductance to water vapour (gs) were measured. At the end of each season, the yield, kernel unit weight and irrigation water productivity were determined. Significant differences in physiological behaviour and yield responses among cultivars were found. According to the final yield obtained during the experimental seasons, Guara and Lauranne did not show significant improvements in the 150-ETC in relation to FI and RDI65, whereas cv. Marta showed significant improvements under 150-ETC. Moreover, yield response followed the physiological trend observed in the different irrigation treatments for each cultivar. Those cultivars (Guara and Lauranne) that had not shown significant differences in terms of yield, evidenced a similar pattern in terms of gs, unlike Marta. The obtained results allow us to conclude that there is a clear differential response in terms of the cultivar, taking into account the adaptation capacity of different cultivars to different irrigation doses.

Regulation of the cellular metabolism is now recognized as a crucial mechanism for the homeostasis of innate and adaptive immune cells upon diverse extracellular stimuli. Macrophages, for instance, increase glycolysis upon stimulation with pathogen-associated molecular patterns (PAMPs). Conceivably, pathogens also counteract these metabolic changes for their own survival in the host. However, despite this dynamic interplay in host-pathogen interactions, the role of immunometabolism in the context of intracellular bacterial infections is still unclear. Here, employing unbiased metabolomic and transcriptomic approaches, we investigated the role of metabolic adaptations of macrophages upon Salmonella enterica serovar Typhimurium (S. Typhimurium) infections. Importantly, our results suggested that S. Typhimurium abrogates glycolysis and its modulators such as insulin-signaling to impair macrophage defense. Mechanistically, glycolytic enzyme aldolase A is critical for v-ATPase assembly and the acidification of phagosomes upon S. Typhimurium infection, and impairment in the glycolytic machinery eventually leads to decreased bacterial clearance and antigen presentation in macrophages. Collectively, our results highlight a vital molecular link between metabolic adaptation and phagosome maturation in macrophages, which is targeted by S. Typhimurium to evade cell-autonomous defense.

TFEB is an important transcriptional regulator of autophagy, lysosome biogenesis, and immunity, whose activity is regulated by cytosol-to-nuclear translocation through downregulation of the nutrient sensor mTOR. Here we show that TFEB has a non-transcriptional role in mitochondria essential for the regulation of mitochondrial complex I and inflammation. Proteomic analysis revealed that TFEB co-precipitates with several mitochondrial proteins, whose interactions are perturbed in S. Typhimurium infected cells. High resolution microscopy and biochemical experiments further confirmed that TFEB localizes in the mitochondrial matrix. TFEB mitochondrial translocation depends on a conserved TOMM20 binding motif within the protein sequence and is enhanced upon the inhibition of mTOR. Within the mitochondria, TFEB and protease LONP1 antagonistically co-regulate complex I, ROS production and the inflammatory response. Consequently, during S. Typhimurium infection, lack of TFEB specifically in the mitochondria exacerbates the expression of pro-inflammatory cytokines, contributing to pathogenesis.

Overconsumption of food is a major health concern in the western world. Palatable food has been shown to alter the activity of neural circuits, and obesity has been linked to alterations in the connectivity between the hypothalamus and cortical regions involved in decision-making and reward processing, putatively modulating the incentive value of food. Outlining neurophysiological adaptations induced by dietary intake of high fat diets (HFD) is thus valuable to establish how the diet by itself may promote overeating. To this end, C57BL/6 mice were fed HFD rich in either saturated fatty acids (HFD-S) or polyunsaturated fatty acids (HFD-P), or a low-fat control diet (LFD) for four weeks. Food and energy intake were monitored and ex vivo electrophysiology was employed to assess neuroadaptations in lateral hypothalamus (LH) and corticostriatal circuits, previously associated with food intake. In addition, the effects of dietary saturated and polyunsaturated fatty acids on the gene expression of NMDA, AMPA and GABAA receptor subunits in the hypothalamus were investigated. Our data shows that mice fed HFD-P had increased daily food and energy intake compared with mice fed HFD-S or LFD. However, this increase in energy intake had no obesogenic effects. Electrophysiological recordings demonstrated that HFD-P had a selective effect on glutamatergic neurotransmission in the LH, which was concomitant with a change in mRNA expression of AMPA receptor subtypes Gria1, Gria3 and Gria4, with no effect on the mRNA expression of NMDA receptor subtypes or GABAA receptor subtypes. Furthermore, while synaptic output from corticostriatal subregions was not significantly modulated by diet, synaptic plasticity in the form of long-term depression (LTD) was impaired in the dorsomedial striatum of mice fed HFD-S. In conclusion, this study suggests that the composition of fatty acids in the diet not only affects weight gain, but may also modulate neuronal function and plasticity in brain regions involved in food intake.

Deficit irrigation (DI) strategies are considered essential in many arid and semi-arid areas of Mediterranean countries for proper water management under drought conditions. This fact is even more necessary in crops such as almond (Prunus dulcis Mill.), which in the last recent years has been progressively introduced in irrigated areas. An essential aspect to be considered would be the ability to improve fruit-quality parameters when DI strategies are imposed, which can boost the final almond price and ensure the sustainability and competitiveness of this crop. This work examines the effects of sustained deficit irrigation (SDI) on three almond cultivars (Marta, Guara, and Lauranne) on parameters related to almond functionality, aroma and sensory profile, which consequently influence its marketability and consumers acceptance. SDI strategies allowed the improvement of physical parameters such as unit weight, kernel length, kernel thickness or color. Moreover, higher total phenolic compounds, organic acids and sugars were found in SDI almonds. Finally, the highest concentrations of volatile compounds were obtained under SDI, this being a clear advantage in relation to almond flavor. Thus, moderate SDI strategy offered relevant improvements in parameters regarding the marketability, by enhancing the final added value of hydroSOStainable almonds with respect to those cultivated under full irrigation conditions.

VEGFR2 and VEGF-A play a pivotal role in the process of angiogenesis. VEGFR2 activation is regulated by protein tyrosine phosphatases (PTPs), enzymes that dephosphorylate the receptor and reduce angiogenesis. We aim to study the effect of PTPs blockade using bis(maltolato)oxovanadium(IV) (BMOV) on in vivo wound healing and in vitro angiogenesis. BMOV significantly improves in vivo wound closure by 45% in C57BL/6JRj mice. We found that upon VEGFR2 phosphorylation induced by endogenously produced VEGF-A, the addition of BMOV results in increased cell migration (45%), proliferation (40%) and tube formation (27%) in HUVECs compared to control. In a mouse ex vivo, aortic ring assay BMOV increased the number of sprouts by 3 folds when compared to control. However, BMOV coadministered with exogenous VEGF-A increased ECs migration, proliferation and tube formation by only 41%, 18% and 12% respectively and aortic ring sprouting by only 1-fold. We also found that BMOV enhances VEGFR2 Y951 and p38MAPK phosphorylation, but not ERK1/2. The level of phosphorylation of these residues was the same in the groups treated with BMOV supplemented with exogenous VEGF-A and exogenous VEGF-A only. Our study demonstrates that BMOV is able to enhance wound closure in vivo. Moreover, in the presence of endogenous VEGF-A, BMOV is able to stimulate in vitro angiogenesis by increasing the phosphorylation of VEGFR2 and its downstream proangiogenic enzymes. Importantly, BMOV had a stronger proangiogenic effect compared to its effect in coadministration with exogenous VEGF-A.

The composition of the diet affects many processes in the body, including body weight and endocrine system. We have previously shown that dietary fat also affects the immune system. Mice fed high fat diet rich in polyunsaturated fatty acids survive S. aureus infection to a much greater extent than mice fed high fat diet rich in saturated fatty acids. Here we present data regarding the dietary effects on protein expression in spleen from mice fed three different diets, I) low fat/chow diet (LFD, n = 4), II) high fat diet rich in saturated fatty acids (HFD-S, n = 4) and III) high fat diet rich in polyunsaturated fatty acids (HFD-P, n = 4). We performed mass spectrophotometry based quantitative proteomics analysis of isolated spleen by implementing the isobaric tags for relative and absolute quantification (iTRAQ) approach. Mass spectrometry data were analyzed using Proteome Discoverer 2.4 software using the search engine mascot against Mus musculus in SwissProt. 924 proteins are identified in all sets (n = 4) for different dietary effects taken for statistical analysis using Qlucore Omics Explorer software. Only 20 proteins were found to be differentially expressed with a cut-off value of false discovery rate < 0.1 (q-value) when comparing HFD-S and HFD-P but no differentially expressed proteins were found when LFD was compared with HFD-P or HFD-S. The identified proteins and statistical analysis comparing HFD-S and HFD-P diets are available as a supplementary file S1. We identified a subset of proteins that showed an inverse expression pattern between two high fat diets. These differentially expressed proteins were further classified by gene ontology for their role in biological processes and molecular functions. Mass spectrometry raw data are also available via ProteomeXchange with identifier PXD020365.

Almond (Prunus dulcis Mill. (D.A. Webb)) plantations in irrigated semi-arid areas need to successfully face the new scenarios of climate change combining sustainable irrigation strategies and tolerant cultivars to water stress. This work examines the response of young almond (cvs. Guara, Marta, and Lauranne) subjected to different irrigation doses under semi-arid conditions (South-West Spain). The trial was conducted during two seasons (2018&ndash, 2019) with three irrigation strategies: A full-irrigated treatment (FI), which received 100% of the irrigation requirements (IR), and two sustained-deficit irrigation strategies that received 75% (SDI75) and 65% (SDI65) of IR. Crop water status was assessed by leaf water potential (&Psi, leaf) and stomatal conductance (gs) measurements, determining the yield response at the end of each season. Different physiological responses for the studied cultivars were observed, especially considering the &Psi, leaf measurements. In this way, cv. Marta behaved more tolerant, while cvs. Guara and Lauranne maintained higher gs rates in response to water stress. These differences were also observed in terms of yield. The cv. Lauranne did not reflect yield losses, and the opposite trend was observed for cv. Guara, in which reductions on fruit numbers per tree were detected. On overall, effective irrigation water savings (&asymp, 2100 m3&middot, ha&minus, 1 in SDI65) could be feasible, although these responses are going to be substantially different, depending on the used cultivar.

This work examines the use of thermal imaging to determine the crop water status in young almond trees under sustained deficit irrigation strategies (SDIs). The research was carried out during two seasons (2018&ndash, 2019) in three cultivars (Prunus dulcis Mill., cvs. Guara, Lauranne, and Marta) subjected to three irrigation treatments: a full irrigation treatment (FI) at 100% of irrigation requirements (IR), and two SDIs that received 75% and 65% of the IR, respectively. Crop water monitoring was done by measurements of canopy temperature, leaf water potential (&Psi, leaf), and stomatal conductance. Thermal readings were used to define the non-water-stress baselines (NWSB) and water-stress baselines (WSB) for each treatment and cultivar. According to our findings, &Psi, leaf was the most responsive parameter to reflect differences in almond water status. In addition, NWSB and WSB allowed the determination of the crop water-stress index (CWSI) and the increment of canopy temperature (ITC) for each SDI treatment, obtaining threshold values of CWSI (0.12&ndash, 0.15) and ITC (~1 &deg, C) that would ensure maximum water savings by minimizing the effects on yield. The findings highlight the importance of determining the different NWSB and WSB for different almond cultivars and its potential use for proper irrigation scheduling.

Regulation of the cellular metabolism is now recognized as a crucial mechanism for the homeostasis of innate and adaptive immune cells upon diverse extracellular stimuli. Macrophages, for instance, increase glycolysis upon stimulation with pathogen-associated molecular patterns (PAMPs). Conceivably, pathogens also counteract these metabolic changes for their own survival in the host. However, despite this dynamic interplay in host-pathogen interactions, the role of immunometabolism in the context of intracellular bacterial infections is still unclear. Here, employing unbiased metabolomic and transcriptomic approaches, we investigated the role of metabolic adaptations of macrophages upon Salmonella enterica serovar Typhimurium (S. Typhimurium) infections. Importantly, our results suggested that S. Typhimurium abrogates glycolysis and its modulators such as insulin-signaling to impair macrophage defense. Mechanistically, glycolytic enzyme aldolase A is critical for v-ATPase assembly and the acidification of phagosomes upon S. Typhimurium infection, and impairment in the glycolytic machinery eventually leads to decreased bacterial clearance and antigen presentation in macrophages. Collectively, our results highlight a vital molecular link between metabolic adaptation and phagosome maturation in macrophages, which is targeted by S. Typhimurium to evade cell-autonomous defense.

Climate change, water resources depletion, or land degradation and abandonment are some constraints to overcome within the new paradigm of achieving competitive and sustainable agriculture. Within the possible strategies, the introduction of drought-tolerant crops with high profitability, and the implementation of water-saving strategies such as deficit irrigation (DI) should be considered. Within the crop possibilities, almond (Prunus dulcis Mill.) would be an excellent alternative under water scarcity and climate change scenarios. However, it is essential to reach an equilibrium between crop management and water availability, defining its water requirements and the relationships between irrigation needs and the agronomical practices. Once done, adaptation strategies to water scarcity should be considered, especially in those cases of lack to cover the total irrigation needs by DI practices. In this agreement, the success of these strategies will depend on the proper knowledge respect to crop phenological development and the assessment of physiological status using different available tools. Thus, different crop physiological responses to water stress will be observed and different taking decisions should be considered. Finally, implementing water-saving strategies will be done not only from the perspective of preserving maximum yields but also for product quality improvements, and hence providing a final added value.

Almond (Prunnus dulcis Mill.) has been traditionally associated to marginal land cultivation and rain-fed agriculture in South Spain. However, in the last years, this crop is being progressively introduced in more productive agricultural areas within the Guadalquivir river basin, where the available water resources are not enough to satisfy the adequate crop-water requirements. Considering this limitation, a more precise irrigation scheduling to maximize the yield is required. Infrared thermal imaging emerges as alternative to other traditional methodologies to assess the crop-water status, especially when deficit irrigation (DI) strategies are being applied. The aim of this study was to define the methodology to assess the almond water status by means of thermal information. The trial was conducted during 2014, during the kernel-filling period, in an almond experimental orchard (SW Spain), with 5-year-old trees, subjected to three irrigation regimes: i) a full-irrigation treatment (C-100), which received 100% of ETC; ii) a regulated deficit irrigation (RDI-50), which received 100% of ETC except during the kernel filling period, when this treatment was irrigated with 50% of ETC; iii) and a low-frequency deficit irrigation treatment (LFDI), which received 100% of ETC except during the kernel filling period, when it was subjected to continuous periods of irrigation-restriction, defined in terms of the threshold values of shaded leaf water potential (Ψleaf). Three daily curves of canopy temperature (TC), stomatal conductance to water vapour (gs) and Ψleaf with measurements at 8:00, 11:00, 14:00, 17:00 and 20:00 were developed. Additionally, Crop Water Stress Index (CWSI), temperature difference between canopy and the surrounding air (ΔTcanopy-air), and the relative index to stomatal conductance (IG) obtained at different scales (canopy and row) were estimated. Significant correlations of infrared thermal information vs. Ψleaf and gs were obtained (p ≤ 0.05 and p ≤ 0.01), in particular, by using the thermal readings taken at 11:30, 14:30 at 17:30 h, especially robust were the relationships obtained between TC and CWSI with Ψleaf at 11:30 h; and between TC and CWSI with gs, and Ψleaf at 14:30 h. Finally, considering the infrared thermal monitoring procedure (readings at tree and row level), similar values of TC were obtained, and therefore, the images taken at row level offered a better information with a higher feasibility in terms of image processing.

Mango (Mangifera indica L.) is one of the most important fruit crops in tropical and subtropical regions worldwide. On the coast of Granada and Malaga (SE Spain), irrigated subtropical fruit specie...

This work examines the long-term effects of deficit-irrigation (DI) practices in almond crop (Prunus dulcis Mill.) in agronomical and physiological terms. The trial was conducted during four-year monitoring period (2014–2017), in an experimental orchard (SW Spain), subjected to three irrigation regimes; i) a full-irrigation treatment (FI), which received 100% of crop evapotranspiration (ETC); ii) a regulated-deficit irrigation (RDI50), which received 50% of ETC during the kernel-filling period; and iii) a low-frequency deficit irrigation (LFDI), that was subjected to continuous periods of irrigation-restriction defined in terms of threshold values of leaf-water potential (Ψleaf) during the kernel-filling period. During the water stress period, there were monitored Ψleaf, stomatal conductance (gs) and canopy temperature (TC). Significant improvements in terms of water-use efficiency were found, as no differences in terms of yield between FI and LFDI were found, leading to the conclusion that signif...

Terracing is a soil conservation strategy applied worldwide to prevent erosion and runoff on sloping lands. Orchard terraces can considerably reduce soil loss due to water erosion if they are well planned, correctly constructed and properly maintained. Terraces have to be combined with additional soil conservation practices, of which the most important is the maintenance of a soil cover, especially during the rainy period. On the coastal strip of the provinces of Granada and Malaga (south-eastern Spain), irrigated subtropical fruit species have been introduced and cultivated on terraces with a considerable importance as the only European producer region. The subtropical farming in this zone also has strong socio-economic impact. In the present chapter, land-use changes were analysed in a selected representative watershed over 29 years. According to the findings, formerly, 97.5% of the watershed was devoted to traditional Mediterranean crops; however, after this period, due to abandonment, this area was reduced to 17.6% and increased in subtropical fruit crops (26.6%), shrubland (29.8%) and abandoned cropland (24.6%). The main driving force in land-use change has been intensive irrigation on terraces planted with subtropical crops, which are economically more profitable than traditional rainfed crops, almond and olive, which have been replaced or abandoned. The intensification of subtropical farming in terraces provokes environmental effects, especially those regarding soil and water resources, which need to be minimized. The results support the recommendation of using plant covers on the taluses of subtropical crop terraces in order to control soil erosion and improve the soil quality in the taluses of orchard terraces. In this sense, compared to bare soil, thyme and native spontaneous vegetation plant covers reduced the runoff with 94% and 93% and declined erosion with 71% and 79%, respectively. That is to avoid the collapse of the structure and make more feasible the subtropical fruit cultivation in the study area. Thus, it is possible to mitigate the impact of subtropical farming on terraces by adopting sustainable measures for soil and water conservation.

Significance In the present study, we identify and describe an important cross-talk between leptin signaling and macrophage functions in the context of Salmonella Typhimurium infection. Genetic ablation of leptin receptor or pharmacological antagonization of leptin augmented lysosomal functions in macrophages, reduced S. Typhimurium burden, and diminished inflammation both in vitro and in vivo. Leptin signaling activates mTORC2/Akt pathway through the down-regulation of Phlpp1 phosphatase, thus impairs lysosome-mediated pathogen clearance., The dynamic interplay between metabolism and immune responses in health and disease, by which different immune cells impact on metabolic processes, are being increasingly appreciated. However, the potential of master regulators of metabolism to control innate immunity are less understood. Here, we studied the cross-talk between leptin signaling and macrophage function in the context of bacterial infections. We found that upon infection with Gram-negative pathogens, such as Salmonella Typhimurium, leptin receptor (Lepr) expression increased in both mouse and human macrophages. Unexpectedly, both genetic Lepr ablation in macrophages and global pharmacologic leptin antagonization augmented lysosomal functions, reduced S. Typhimurium burden, and diminished inflammation in vitro and in vivo. Mechanistically, we show that leptin induction activates the mTORC2/Akt pathway and subsequently down-regulates Phlpp1 phosphatase, allowing for phosphorylated Akt to impair lysosomal-mediated pathogen clearance. These data highlight a link between leptin signaling, the mTORC2/Phlpp1/Akt axis, and lysosomal activity in macrophages and have important therapeutic implications for modulating innate immunity to combat Gram-negative bacterial infections.

Water scarcity in many semi-arid agricultural areas, in particular for the Mediterranean basin, is promoting changes in irrigated agriculture, with alternative strategies being introduced for water-use optimization. The coast of Granada and Malaga (Southeast Spain) is an economically important area for subtropical fruit cultivation. This intensively irrigated agriculture is characterized by requiring extra amounts of water and the adoption of sustainable practices to improve agricultural water management. A two-season experiment was conducted to assess (1) the water use in terraced cherimoya (Annona cherimola Mill. cv. Fino de Jete) orchards under conventional and organic production systems with drainage lysimeters, and (2) the impact on fruit yield and nutritional effects between the two considered production systems. Crop coefficient (Kc) values for cherimoya were 0.60−0.66, 0.64−0.71, and 0.48−0.62 at flowering, fruit set, and fruit growth, respectively. Fruit yield was similar in both systems, ranging from 47.1 for conventional to 44.1 kg tree−1 for organic farming, averaging 13.2 and 12.3 t·ha−1, respectively. No differences between these systems were observed in terms of leaf nutrient status, with variations in the N, P, and K contents during the different phenological stages. The N, P, and K lessen during flowering and fruit growth; the highest levels of these nutrients were fixed at harvest. These patterns were the opposite in Ca and Mg, ascribable to the antagonism between K and both Ca and Mg. Thus, these findings highlight the need to establish the optimal use of irrigation water with respect to crop requirements, thereby encouraging sustainable subtropical farming in terraces.

Water is the most limiting resource in many semi-arid areas of Mediterranean countries. Among the strategies to improve water productivity, the implementation of deficit irrigation (DI) strategies and the introduction of drought-tolerant crops in irrigated areas (such as almond) are being widely studied. Recently, the use of biostimulants to enhance crop tolerance to drought under water-scarcity scenarios is increasing. This work examines the response of three almond cultivars (&lsquo, Guara&rsquo, &lsquo, Marta&rsquo, and &lsquo, Lauranne&rsquo, ) in terms of yield and associated physiological responses in the main phenological stages to biostimulants (HYT&reg, A and HYT&reg, B plus) applied to young trees subjected to different irrigation levels: (i) a full irrigation treatment (FI), irrigated at 100% of crop evapotranspiration (ETC), and (ii) sustained-deficit irrigation (SDI75), irrigated at 75% of ETC. Significantly higher yields were obtained with HYT applications in 2 of 3 cultivars, these differences were most evident in the SDI75 treatment. In particular, &lsquo, registered the most significant improvements in nut yield when the HYT product was applied (15&ndash, 20% higher). With regard to crop physiological responses, higher values of leaf water potential and stomatal conductance were noted with the HYT application in some cultivars and phenological stages. These results indicated that the use of biostimulants can be a feasible strategy for almond cultivation, especially when SDI is used.

Neutrophils are the most abundant circulating leukocytes in humans and are essential for the defense against invading pathogens. Like many other cells of an organism, neutrophils can be highly influenced by the diet. We have previously described that mice fed a high-fat diet rich in polyunsaturated fatty acids (HFD-P) present a higher frequency of neutrophils in bone marrow than mice fed a high-fat diet rich in saturated fatty acids (HFD-S). Interestingly, such an increase correlated with improved survival against bacterium-induced sepsis. In this study, we aimed to investigate the effects of dietary polyunsaturated and saturated fatty acids on neutrophil homeostasis. We found that HFD-P specifically induced the accumulation of neutrophils in the marginal pools of the spleen and liver. The accumulation of neutrophils in the spleen was a result of a dual effect of polyunsaturated fatty acids on neutrophil homeostasis. First, polyunsaturated fatty acids enhanced the recruitment of neutrophils from the circulation into the spleen via chemokine secretion. Second, they delayed neutrophil cell death in the spleen. Interestingly, these effects were not observed in mice fed a diet rich in saturated fatty acids, suggesting that the type of fat rather than the amount of fat mediates the alterations in neutrophil homeostasis. In conclusion, our results show that dietary polyunsaturated fatty acids have a strong modulatory effect on neutrophil homeostasis that may have future clinical applications.

Background and aims Alpha 7 nicotinic acetylcholine receptor (α7nAChR) stimulation can regulate acute inflammation, and lack of α7nAChR accelerates atherosclerosis in mice. In this study, we aimed to investigate the effects of the novel α7nAChR agonist, AZ6983, on atherosclerosis and assess its possible immune-modulating effects. Methods AZ6983 was tested in vitro in LPS-challenged mouse and human blood and in vivo using the acute inflammatory air pouch model. Thereafter, long-term effects of AZ6983 treatment on atherosclerosis and immune responses were assessed in apoE −/− mice after 8 and 12 weeks. Atherosclerosis was investigated in the aortic root and thoracic aorta, serum levels of cytokines were analysed and RNAseq was used to study aortic gene expression. Further, bone-marrow-derived macrophages were used to assess phagocytosis in vitro . Results α7nAChR activation by AZ6983 decreased pro-inflammatory cytokines in acute stimulations of human and mouse blood in vitro , as well as in vivo using the air pouch model. Treating apoE −/− mice with AZ6983 decreased atherosclerosis by 37–49% and decreased serum cytokine levels. RNAseq analysis of aortae suggested the involvement of several specific myeloid cell functions, including phagocytosis. In line with this, AZ6983 significantly increased phagocytosis in bone marrow-derived macrophages. Conclusions This study demonstrates that activation of α7nAChR with AZ6983 inhibits atherosclerosis in apoE −/− mice and that immune-modulating effects on myeloid cells, such as enhanced phagocytosis and suppression of inflammatory cytokines, could be part of the athero-protective mechanisms. The observed anti-inflammatory effect in human blood supports the idea that AZ6983 may decrease disease also in humans.

El almendro ha ido introduciéndose en los últimos años progresivamente en el valle del Guadalquivir en condiciones de regadío como alternativa a otros cultivos tradicionales con una menor rentabilidad. Por ello existe una importante demanda de conocimientos en relación con las estrategias de riego más adecuadas para este cultivo, especialmente cuando las dotaciones hídricas están por debajo del óptimo demandado por el cultivo. Este trabajo presenta los resultados más relevantes en torno a las líneas base de potencial para la gestión del riego en tres variedades tradicionales de almendro, con una amplia representación en el valle del Guadalquivir. Estas líneas base de potencial permitieron definir el rango óptimo, así como los valores umbrales de potencial hídrico foliar para cada una de las variedades en función de la disponibilidad hídrica existente, permitiendo realizar una toma de decisiones adecuada, especialmente en situaciones de escasez de agua, minimizando las pérdidas en producción.

The Mediterranean region is one of the most water-scarce areas worldwide and is considered a climate-change hotspot. To assure the viability and competitiveness of irrigated agriculture, it is vital to implement strategies that can maximize water saving without compromising yield. Deficit irrigation (DI) for cultivating drought-tolerant species such as almond (Prunus dulcis (Mill.) D.A. Webb) can help in achieving this goal, while at the same time improving fruit chemical composition. This work evaluated the effect of DI techniques and cultivars on the chemical composition of almonds (cvs. Marta, Guara, and Lauranne) in order to elucidate the most suitable irrigation dose under water-scarcity scenarios. Three irrigation regimes were imposed: a control treatment (FI), which was fully irrigated, receiving 100% of the irrigation requirement (IR), and two sustained-deficit irrigation (SDI) strategies that received 75% (SDI75) and 65% (SDI65) of IR. Significant differences among cultivars and irrigation treatments were observed for antioxidant activity and organic acid, sugar, and fatty acid content, which were increased by the SDI strategies. In addition, highly significant correlations were found between leaf-water potential and components such as fumaric acid, sugars, and fatty acids. In terms of the cultivars, cv. Marta showed the highest antioxidant activity, cv. Guara was the richest in organic acids, and cv. Lauranne had the highest fatty acid content. Consequently, SDI strategies improved almond quality parameters related to their nutritional and sensory composition, with significant water savings (reductions of 25&ndash, 35%) and without important yield loss.

Salmonella typhimurium is a facultative intracellular bacterium that causes gastroenteritis in humans. After invasion of the lamina propria, S. typhimurium bacteria are quickly detected and phagocytized by macrophages, and contained in vesicles known as phagosomes in order to be degraded. Isolation of S. typhimurium-containing phagosomes have been widely used to study how S. typhimurium infection alters the process of phagosome maturation to prevent bacterial degradation. Classically, the isolation of bacteria-containing phagosomes has been performed by sucrose gradient centrifugation. However, this process is time-consuming, and requires specialized equipment and a certain degree of dexterity. Described here is a simple and quick method for the isolation of S. typhimurium-containing phagosomes from macrophages by coating the bacteria with biotin-streptavidin-conjugated magnetic beads. Phagosomes obtained by this method can be suspended in any buffer of choice, allowing the utilization of isolated phagosomes for a broad range of assays, such as protein, metabolite, and lipid analysis. In summary, this method for the isolation of S. typhimurium-containing phagosomes is specific, efficient, rapid, requires minimum equipment, and is more versatile than the classical method of isolation by sucrose gradient-ultracentrifugation.

Salmonella typhimurium is a facultative intracellular bacterium that causes gastroenteritis in humans. After invasion of the lamina propria, S. typhimurium bacteria are quickly detected and phagocytized by macrophages, and contained in vesicles known as phagosomes in order to be degraded. Isolation of S. typhimurium-containing phagosomes have been widely used to study how S. typhimurium infection alters the process of phagosome maturation to prevent bacterial degradation. Classically, the isolation of bacteria-containing phagosomes has been performed by sucrose gradient centrifugation. However, this process is time-consuming, and requires specialized equipment and a certain degree of dexterity. Described here is a simple and quick method for the isolation of S. typhimurium-containing phagosomes from macrophages by coating the bacteria with biotin-streptavidin-conjugated magnetic beads. Phagosomes obtained by this method can be suspended in any buffer of choice, allowing the utilization of isolated phagosomes for a broad range of assays, such as protein, metabolite, and lipid analysis. In summary, this method for the isolation of S. typhimurium-containing phagosomes is specific, efficient, rapid, requires minimum equipment, and is more versatile than the classical method of isolation by sucrose gradient-ultracentrifugation.

During intracellular infections, autophagy significantly contributes to the elimination of pathogens, regulation of pro-inflammatory signaling, secretion of immune mediators and in coordinating the adaptive immune system. Intracellular pathogens such as S. Typhimurium have evolved mechanisms to circumvent autophagy. However, the regulatory mechanisms targeted by S. Typhimurium to modulate autophagy have not been fully resolved. Here we report that cytosolic energy loss during S. Typhimurium infection triggers transient activation of AMPK, an important checkpoint of mTOR activity and autophagy. The activation of AMPK is regulated by LKB1 in a cytosolic complex containing Sirt1 and LKB1, where Sirt1 is required for deacetylation and subsequent activation of LKB1. S. Typhimurium infection targets Sirt1, LKB1 and AMPK to lysosomes for rapid degradation resulting in the disruption of the AMPK-mediated regulation of mTOR and autophagy. The degradation of cytosolic Sirt1/LKB1/AMPK complex was not observed with two mutant strains of S. Typhimurium, ΔssrB and ΔssaV, both compromising the pathogenicity island 2 (SPI2). The results highlight virulence factor-dependent degradation of host cell proteins as a previously unrecognized strategy of S. Typhimurium to evade autophagy., Author summary S. Typhimurium is a facultative intracellular pathogen which uses its type III secretion system to avoid cell-autonomous defense mechanisms such as autophagy. Here we show that S. Typhimurium induces energy depletion resulting in an early but transient activation of AMPK and autophagy. Salmonella virulence factors target Sirt1/LKB1/AMPK for lysosomal degradation, which enables sustained mTOR-activation and inhibition of autophagy. Activation of mTOR establishes a molecular feedback loop that enhances lysosomal degradation of Sirt1/LKB1/AMPK.

Type I interferon (IFN-I) triggers necroptosis in macrophages infected with S. Typhimurium by an unclear mechanism. Hos et al. now demonstrate that RIP3 enhances the interaction of Nrf2 with Pgam5 in response to IFN-I signaling in S. Typhimurium–infected macrophages, which abates Nrf2-dependent cytoprotective pathways and increases cell death., Salmonella enterica serovar Typhimurium exploits the host’s type I interferon (IFN-I) response to induce receptor-interacting protein (RIP) kinase–mediated necroptosis in macrophages. However, the events that drive necroptosis execution downstream of IFN-I and RIP signaling remain elusive. In this study, we demonstrate that S. Typhimurium infection causes IFN-I–mediated up-regulation of the mitochondrial phosphatase Pgam5 through RIP3. Pgam5 subsequently interacts with Nrf2, which sequesters Nrf2 in the cytosol, thereby repressing the transcription of Nrf2-dependent antioxidative genes. The impaired ability to respond to S. Typhimurium–induced oxidative stress results in reactive oxygen species–mediated mitochondrial damage, energy depletion, transient induction of autophagy, and autophagic degradation of p62. Reduced p62 levels impair interaction of p62 with Keap1, which further decreases Nrf2 function and antioxidative responses to S. Typhimurium infection, eventually leading to cell death. Collectively, we identify impaired Nrf2-dependent redox homeostasis as an important mechanism that promotes cell death downstream of IFN-I and RIP3 signaling in S. Typhimurium–infected macrophages.

The spontaneous heterotopic pregnancy is an unusual event. Generally the extrauterine product lost and the intrauterine present complications in different grade. The most common is the rupture and can be associated with hemodynamic unstable with risk for patient's life. The intrauterine pregnancy can be continuing to the end. We report the case of patient with spontaneous heterotopic pregnancy; the diagnosis was in the first trimester, with two products (intrauterine and in right fimbrius). The salpingectomy is doing with the extrauterine pregnancy and the patient has not complications trans or postoperative time and without complications for the intrauterine pregnancy, she continues with stricter control until to obtain a life product.