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148 results on '"Saras, Estelle"'

3. Healthcare Equipment and Personnel Reservoirs of Carbapenem-Resistant Acinetobacter baumannii Epidemic Clones in Intensive Care Units in a Tunisian Hospital

Author

Azaiez, Sana, primary, Haenni, Marisa, additional, Cheikh, Asma Ben, additional, Chalbi, Mohamed Sahbi, additional, Messaoudi, Aziza, additional, Tilouch, Lamia, additional, Bahri, Sana, additional, Drapeau, Antoine, additional, Saras, Estelle, additional, Mtibâa, Mariem, additional, Zouaoui, Rania, additional, Said, Houyem, additional, Madec, Jean-Yves, additional, Lupo, Agnese, additional, and Mansour, Wejdene, additional

Published
2023
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11. Investigation of an XDR- Acinetobacter Baumannii ST2 Outbreak in an Intensive Care Unit of a Lebanese Tertiary Care Hospital

Author

Osman, Marwan, primary, B Halimeh, Fatima, additional, Rafei, Rayane, additional, Mallat, Hassan, additional, Tom, Jad El, additional, Raad, Elie B, additional, M Diene, Seydina, additional, Jamal, Sabah, additional, Atrouni, Ahmad Al, additional, Dabboussi, Fouad, additional, Moudani, Walid, additional, Madec, Jean-Yves, additional, Saras, Estelle, additional, Lupo, Agnese, additional, Haenni, Marisa, additional, Rolain, Jean-Marc, additional, and Hamze, Monzer, additional

Published
2020
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14. High Genetic Diversity of Enterobacteriaceae Clones and Plasmids Disseminating Resistance to Extended-Spectrum Cephalosporins and Colistin in Healthy Chicken in Tunisia

Author

Saidani, Mariem, primary, Messadi, Lilia, additional, Chaouechi, Amira, additional, Tabib, Ines, additional, Saras, Estelle, additional, Soudani, Alya, additional, Daaloul-Jedidi, Monia, additional, Mamlouk, Aymen, additional, Ben Chehida, Faten, additional, Chakroun, Chahid, additional, Madec, Jean-Yves, additional, and Haenni, Marisa, additional

Published
2019
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17. Is blaCTX-M-1 Riding the Same Plasmid Among Horses in Sweden and France?

Author

Lupo, Agnese, Haenni, Marisa, Saras, Estelle, Gradin, Joanna, Madec, Jean-Yves, Börjesson, Stefan, Lupo, Agnese, Haenni, Marisa, Saras, Estelle, Gradin, Joanna, Madec, Jean-Yves, and Börjesson, Stefan

Abstract

A predominance of the blaCTX-M-1/IncHI1 plasmid combination in horses has been reported in Czech-Republic, Denmark, and The Netherlands. To clarify a possible specific plasmid epidemiology of blaCTX-M-1 in horses in a European perspective, a collection of 74 extended-spectrum beta-lactamase-producing Escherichia coli recovered from diseased horses in France and Sweden during the period 2009-2014 was investigated in respect of their genetic relatedness, plasmid content, and molecular features. Overall, 80% of E. coli isolates from diseased horses harbored blaCTX-M-1 on large IncHI1 plasmids with plasmid sequence type (pST) 2 and pST9 more prevalent in Sweden and France, respectively. In French isolates, IncI1/pST3 plasmids were also identified. The CTX-M-1-producing E. coli belonged principally to the clonal complex 10. ST641, together with its single locus variant, and ST1730 constituted two other major groups. The rep-PCR clustering highlighted a clonal dissemination of E. coli CTX-M-1 producers in different regions of the same country and during several years. The STs found in our isolates were also reported in The Netherlands, suggesting a common source of contamination in Europe, although only further cooperative investigation will clarify this issue. On the other hand, the spread of IncI1/pST3 plasmids among horses constitutes another worrisome issue considering their successful spread in other animal hosts such as chicken or bovines. Monitoring evolution and propagation of antimicrobial resistance in equine environment is a priority to avoid further propagation of antimicrobial resistance mechanisms threatening human and animal health., This project was supported by a grant from the CoVetLab Association.

Published
2018
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24. Diversity and mobility of integrative and conjugative elements in bovine isolates of Streptococcus agalactiae, S. dysgalactiae subsp. dysgalactiae, and S. uberis

Author

Haenni, Marisa, Saras, Estelle, Bertin, Stephane, Leblond, Pierre, Madec, Jean-Yves, and Payot, Sophie

Subjects
Streptococcus agalactiae -- Genetic aspects, Streptococcus agalactiae -- Physiological aspects, Macrolide antibiotics -- Research, Genetic transformation -- Analysis, Biological sciences
Abstract

Bovine isolates of Streptococcus agalactiae, Streptococcus dysgalactiae subsp. dysgalactiae and Streptococcus uberis were analyzed for the presence of different integrative and conjugative elements (ICEs) and their association with macrolide, lincosamide, and tetracycline resistance. Most of the strains examined were found to carry antibiotic resistance genes but showed no association between the presence of these ICEs and antibiotic resistance and hence could be a vehicle for horizontal gene transfer between streptococci of animal and/or human origin.

Published
2010

25. Outbreak of colistin-resistant carbapenemase-producing Klebsiella pneumoniae in Tunisia

Author

Mansour, Wejdène, primary, Haenni, Marisa, additional, Saras, Estelle, additional, Grami, Raoudha, additional, Mani, Yosra, additional, Ben Haj Khalifa, Anis, additional, el Atrouss, Souhail, additional, Kheder, Mohamed, additional, Fekih Hassen, Mohamed, additional, Boujâafar, Noureddine, additional, Bouallegue, Olfa, additional, and Madec, Jean-Yves, additional

Published
2017
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32. Investigation of an XDR-Acinetobacter BaumanniiST2 Outbreak in an Intensive Care Unit of a Lebanese Tertiary Care Hospital

Author

Osman, Marwan, B Halimeh, Fatima, Rafei, Rayane, Mallat, Hassan, Tom, Jad El, Raad, Elie B, M Diene, Seydina, Jamal, Sabah, Atrouni, Ahmad Al, Dabboussi, Fouad, Moudani, Walid, Madec, Jean-Yves, Saras, Estelle, Lupo, Agnese, Haenni, Marisa, Rolain, Jean-Marc, and Hamze, Monzer

Abstract

Aim:We sought to investigate the genetic epidemiological relatedness of carbapenem-resistant Acinetobacter baumannii(CRAB) strains of a suspected outbreak in a Lebanese tertiary care hospital to implement necessary infection prevention and control measures. Methods:Twenty-eight nonduplicate CRAB isolates detected among hospitalized patients between January 2016 and July 2017 were studied by real-time polymerase chain reaction (PCR), pulsed-field gel electrophoresis and multilocus sequence typing analyses. Results:Twenty-seven isolates harbored blaOXA-23, of which one also carried blaNDM-1. The isolates distributed temporally in two presumably episodes were stratified by pulsed-field gel electrophoresis into many clusters. Although several clones have become endemic in the hospital, we have rapidly implemented appropriate infection prevention and control measures, achieving full eradication from August 2017 to November 2019. Conclusion:We have successfully investigated and controlled a polyclonal outbreak of OXA-23 producing ST2 CRAB.

Published
2020
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33. Dynamics and molecular features of OXA-48-like-producing Klebsiella pneumoniaelineages in a Tunisian hospital

Author

Messaoudi, Aziza, Haenni, Marisa, Bouallègue, Olfa, Saras, Estelle, Chatre, Pierre, Chaouch, Cherifa, Boujâafar, Noureddine, Mansour, Wejdene, and Madec, Jean-Yves

Abstract

•Dynamics of the global epidemiology of OXA-48-like-producing K. pneumoniae in Sahloul Hospital in 2012-2014•Successive dominance of two K. pneumoniaelineage OXA-48-type combinations (ST101/OXA-48 and ST147/OXA-204)

Published
2020
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40. Emergence of blaCTX-M-55 associated with fosA, rmtB and mcr gene variants in Escherichia coli from various animal species in France.

Author

Lupo, Agnese, Saras, Estelle, Madec, Jean-Yves, and Haenni, Marisa

Subjects
*ESCHERICHIA coli, *FOSFOMYCIN, *AMINOGLYCOSIDES, *FLUOROQUINOLONES, *POLYMERASE chain reaction
Abstract

Objectives: In Asian countries, blaCTX-M-55 is the second most common ESBL-encoding gene. blaCTX-M-55 frequently co-localizes with fosA and rmtB genes on epidemic plasmids, which remain sporadic outside Asia. During 2010-13, we investigated CTX-M-55-producing Escherichia coli isolates and their co-resistance to fosfomycin, aminoglycosides, fluoroquinolones and colistin as part of a global survey of ESBLs in animals in France. Methods: blaCTX-M-55, fosA, rmtB and plasmidic quinolone and colistin resistance genes were characterized by PCR, sequencing and hybridization experiments. Plasmids were classified according to their incompatibility groups and subtypes. Genotyping was performed by MLST and repetitive extragenic palindromic sequence-based PCR. Results: Twenty-one E. coli isolates from bovines (n = 16), dogs (n = 2), horses (n = 2) and a monkey harboured blaCTX -M-55, were MDR and belonged to ST744 (n = 9) and 10 other clones. blacTx-M-55 was mostly located on IncF (n = 19), but also on IncI1 (n = 2) plasmids. On IncF33:A1:B1 plasmids, blaCTX-M-55 co-localized with the rmtB and aac(6')-Ib genes and in one isolate with the fosA3 allele. Ten IncF46:A-:B20 plasmids, which were found in different clones from unrelated animals, also carried the mcr-3 gene. blaCTX-M-55-carrying IncF18:A-:B1 plasmids were found in different animal species from distinct locations and periods, and one additionally carried the fosA4 gene. One isolate harboured the mcr-1 gene, which did not co-localize with blaCTX-M-55. Conclusions: A large diversity of E. coli clones and plasmid types supported the spread of blaCTX-M-55, together with atypical resistance genes, in various animal species in France. fosA and rmtB genes are emerging among animals in Europe and this issue is of concern for public health. [ABSTRACT FROM AUTHOR]

Published
2018
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41. Emergence of blaCTX-M-55 associated with fosA, rmtB and mcr gene variants in Escherichia coli from various animal species in France.

Author

Lupo, Agnese, Saras, Estelle, Madec, Jean-Yves, and Haenni, Marisa

Subjects
ANIMAL diseases, ALLELES, AMINOGLYCOSIDES, ANIMAL experimentation, ANTIBIOTICS, CATTLE, ENZYMES, ESCHERICHIA coli, GENETIC polymorphisms, GENETIC techniques, MULTIDRUG resistance, POLYMERASE chain reaction, PRIMATES, QUINOLONE antibacterial agents, FLUOROQUINOLONES, COLISTIN, GENOTYPES
Abstract

Objectives: In Asian countries, blaCTX-M-55 is the second most common ESBL-encoding gene. blaCTX-M-55 frequently co-localizes with fosA and rmtB genes on epidemic plasmids, which remain sporadic outside Asia. During 2010-13, we investigated CTX-M-55-producing Escherichia coli isolates and their co-resistance to fosfomycin, aminoglycosides, fluoroquinolones and colistin as part of a global survey of ESBLs in animals in France.Methods: blaCTX-M-55, fosA, rmtB and plasmidic quinolone and colistin resistance genes were characterized by PCR, sequencing and hybridization experiments. Plasmids were classified according to their incompatibility groups and subtypes. Genotyping was performed by MLST and repetitive extragenic palindromic sequence-based PCR.Results: Twenty-one E. coli isolates from bovines (n = 16), dogs (n = 2), horses (n = 2) and a monkey harboured blaCTX-M-55, were MDR and belonged to ST744 (n = 9) and 10 other clones. blaCTX-M-55 was mostly located on IncF (n = 19), but also on IncI1 (n = 2) plasmids. On IncF33:A1:B1 plasmids, blaCTX-M-55 co-localized with the rmtB and aac(6')-Ib genes and in one isolate with the fosA3 allele. Ten IncF46:A-:B20 plasmids, which were found in different clones from unrelated animals, also carried the mcr-3 gene. blaCTX-M-55-carrying IncF18:A-:B1 plasmids were found in different animal species from distinct locations and periods, and one additionally carried the fosA4 gene. One isolate harboured the mcr-1 gene, which did not co-localize with blaCTX-M-55.Conclusions: A large diversity of E. coli clones and plasmid types supported the spread of blaCTX-M-55, together with atypical resistance genes, in various animal species in France. fosA and rmtB genes are emerging among animals in Europe and this issue is of concern for public health. [ABSTRACT FROM AUTHOR]

Published
2018
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45. Evolution of florfenicol and chloramphenicol resistance in Escherichia coli strains isolated from cattle in France between 1982 and 2002

Author

Meunier, Danièle, Doublet, Benoît, Lazizzerra, Corinne, Saras, Estelle, Baucheron-Monnier, Sylvie, Cloeckaert, Axel, Madec, Jean-Yves, Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2007

47. High prevalence of international ESBL CTX-M-15-producing Enterobacter cloacae ST114 clone in animals.

Author

Haenni, Marisa, Saras, Estelle, Ponsin, Cécile, Dahmen, Safia, Petitjean, Marie, Hocquet, Didier, and Madec, Jean-Yves

Abstract

Objectives: The objective of this study was to characterize ESBL-producing Enterobacter cloacae isolated from animals and to compare their clonal distribution with that of human-related isolates.Methods: Among 635 clinical E. cloacae from horses, dogs and cats collected in France between 2010 and 2013, 36 were resistant to ceftiofur as determined by disc diffusion. ESBL genes were identified by sequencing. Plasmids carrying ESBL-encoding genes were characterized by PCR-based replicon typing, S1-PFGE and Southern blotting. IncHI2 plasmids were subtyped using the plasmid double-locus sequence typing scheme and multiplex amplification of the hipA, smr0092 and smr0183 genes. All E. cloacae were typed by PFGE and MLST. ST clustering was analysed by eBURST.Results: All 36 ceftiofur-resistant E. cloacae produced an ESBL. Their PFGE patterns formed 23 clusters of high similarity and 13 STs and were isolated from epidemiologically unrelated animals (14 horses, 11 dogs and 11 cats) distributed throughout France. ST114, the most prevalent clone in humans, was over-represented in animals (16/36) compared with other human-related clones detected here. The blaCTX-M-15 gene was dominant (66.7%) and mostly carried on IncHI2 plasmids (ST1 subtype). ST114 isolates always produced CTX-M-15.Conclusions: Most ESBL-producing E. cloacae from animals studied here (69.4%) belonged to potentially high-risk clones in humans, in particular ST114 (44.4%). These data raise questions and potential concerns about the transfer of E. cloacae between animals and humans. [ABSTRACT FROM AUTHOR]

Published
2016
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49. Outbreak of colistin-resistant carbapenemase-producing Klebsiella pneumoniaein Tunisia

Author

Mansour, Wejdène, Haenni, Marisa, Saras, Estelle, Grami, Raoudha, Mani, Yosra, Ben Haj Khalifa, Anis, el Atrouss, Souhail, Kheder, Mohamed, Fekih Hassen, Mohamed, Boujâafar, Noureddine, Bouallegue, Olfa, and Madec, Jean-Yves

Abstract

•Dissemination of antibiotic resistance genes associated with conjugative plasmids.•Emergence of colistin-resistant carbapenemase-producing Klebsiella pneumoniaein Tunisia.•First molecular characterisation of colistin resistance mechanism in K. pneumoniaein Tunisia.

Published
2017
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