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Dimensional measurements are fundamental in microfluidic device manufacturing and performance. The main focus of this study is the measurement of the connection port sizes in microfluidic devices and components and, accordingly, the possible existence of fluid leaks determined using the flow rate error. The sizes associated with three different microfluidic systems were determined using laser interferometry and through an optical measuring instrument, with metrological traceability to national length standards. It was possible to infer the method with the greatest accuracy and lowest measurement uncertainty for characterizing this kind of system. In conclusion, the results of this work directly address the current lack of dimensions measuring methods of microfluidic components by providing a comprehensive comparison of different protocols, ultimately suggesting a preferred option for immediate application within the microfluidic industry. [ABSTRACT FROM AUTHOR]

Background: dementia can be defined as a clinical syndrome developed by pathological processes that affect cognitive functioning, which influences the individual's way of perceiving and understanding the meaning of facts and feelings. Stigma is also a very broad concept, but it can be understood as negative attitudes and prejudices towards individuals who are perceived as different, whether because of a diagnosis, condition, or disease. Method: an integrative systematic literature review was developed to analyze the relationship between stigma and dementia in order to give support to the research question. Drawing upon the Database of Descriptors in Health Sciences (Decs/Mesh), the following descriptors were applied: Estigma e demência (Port.), stigma and dementia (Eng.) estigma y demencia (Spa.). No result on the relationship between these descriptors were found at the Cochrane Library, which may confirm the originality of this review. As for the Open Access Documents, no date limit was applied. The databases used were BVS, PsycInfo, Pubmed, Scopus and Web of Science, following the PRISMA methodology. Result: The initial search resulted in 8,127 titles; after the screening and exclusion criteria process, 42 articles were found for reading and categorization: 25 qualitative papers, 12 quantitative, 1 mixed quantitative and qualitative; 4 articles aimed at the validation and analysis of scales and intervention protocols. The articles mainly analyzed people with dementia, their caregivers, and families. All articles, empirical or theoretical, supported the correlation between stigma and dementia Conclusion: the research outcome points out to the possible of analyze stigma in relation to dementia in different cultures, by applying different approach and methods, such as interviews, scales, questionnaires, and observation, among others. Stigma involves beliefs, attitudes, and behaviors which are susceptible to interventions that may improve public awareness of dementia. Further studies and intervention approach are needed to diminish stigma related to dementia. [ABSTRACT FROM AUTHOR]

Thiosemicarbazones are well known for their broad spectrum of action, including antitumoral and antiparasitic activities. Thiosemicarbazones work as chelating binders, reacting with metal ions. The objective of this work was to investigate the in silico , in vitro , and in vivo toxicity and oxidative stress of 2-acetylpyridine- N (4)-orthochlorophenyl thiosemicarbazone (TSC01). The in silico prediction showed good absorption by biological membranes and no theoretical toxicity. Also, the compound did not show cytotoxicity against Hep-G2 and HT-29 cells. In the acute nonclinical toxicological test, the animals treated with TSC01 showed behavioral changes of stimulus of the central nervous system (CNS) at 300 mg/kg. One hour after administration, a dose of 2000 mg/kg caused depressive signs. All changes disappeared after 24 h, with no deaths, which suggest an estimated LD50 of 5000 mg/kg and GSH 5. The group treated with 2000 mg/kg had an increase of water consumption and weight gain in the second week. The biochemical parameters presented no toxicity relevance, and the analysis of oxidative stress in the liver found an increase of lipid peroxidation and nitric oxide. However, histopathological analysis showed organ integrity was maintained without any changes. In conclusion, the results show the low toxicological potential of thiosemicarbazone derivative, indicating future safe use., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2022 Andressa Brito Lira et al.)

The gingival cyst of the adult is an uncommon odontogenic cyst of developmental origin that is believed to represent the soft tissue counterpart of the lateral periodontal cyst. This article describes a case of gingival cyst of the adult, located in the alveolar mucosa on the facial aspect of the mandibular lateral incisor-canine region. The lesion was nodular with a sessile base, nonulcerated, and nonpainful; it was normal in color and measured 5.0 mm in diameter. An excisional biopsy was performed and surgical exploration revealed osseous surface erosion. Histopathological analysis confirmed the clinical hypothesis.