1. Protein supplementation changes gut microbial diversity and derived metabolites in subjects with type 2 diabetes
- Author
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Ilias Attaye, Pierre Bel Lassen, Solia Adriouch, Emilie Steinbach, Rafael Patiño-Navarrete, Mark Davids, Rohia Alili, Flavien Jacques, Sara Benzeguir, Eugeni Belda, Ina Nemet, James T. Anderson, Laure Alexandre-Heymann, Arno Greyling, Etienne Larger, Stanley L. Hazen, Sophie L. van Oppenraaij, Valentina Tremaroli, Katharina Beck, Per-Olof Bergh, Fredrik Bäckhed, Suzan P.M. ten Brincke, Hilde Herrema, Albert K. Groen, Sara-Joan Pinto-Sietsma, Karine Clément, and Max Nieuwdorp
- Subjects
Dietary supplement ,Health sciences ,Human metabolism ,Microbiome ,Science - Abstract
Summary: High-protein diets are promoted for individuals with type 2 diabetes (T2D). However, effects of dietary protein interventions on (gut-derived) metabolites in T2D remains understudied. We therefore performed a multi-center, randomized-controlled, isocaloric protein intervention with 151 participants following either 12-week high-protein (HP; 30Energy %, N = 78) vs. low-protein (LP; 10 Energy%, N = 73) diet. Primary objectives were dietary effects on glycemic control which were determined via glycemic excursions, continuous glucose monitors and HbA1c. Secondary objectives were impact of diet on gut microbiota composition and -derived metabolites which were determined by shotgun-metagenomics and mass spectrometry. Analyses were performed using delta changes adjusting for center, baseline, and kidney function when appropriate.This study found that a short-term 12-week isocaloric protein modulation does not affect glycemic parameters or weight in metformin-treated T2D. However, the HP diet slightly worsened kidney function, increased alpha-diversity, and production of potentially harmful microbiota-dependent metabolites, which may affect host metabolism upon prolonged exposure.
- Published
- 2023
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