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Additional file 3. Table S2: Sensitivity analyses: ICU mortality comparing early versus non-earlyuse of corticosteroids.

Additional file 4. Figure S1: Restricted cubic spline to plot the odds ratio (95% confidenceinterval) of mortality according to the onset day of corticosteroids treatment.

Additional file 1. Methods, Tables S3–6, Figures S2-5 and References.

Additional file 2. Table S1: Use of corticosteroids and combination of treatments.

Additional file 1: Table 1. Baseline characteristics of patients with HFNO before and after adjustment. Table 2. Clinical evolution of patients with HFNO (maximum or minimum values) before and after adjustment. Table 3. Baseline characteristics of patients with HFNO plus awake prone positioning before and after adjustment. Table 4. Clinical evolution (maximum or minimum values) of patients with HFNO plus awake prone position before and after adjustment. Table 5. Outcomes of the original-eligible population and weighted population. Figure 1. Peripheral oxyhemoglobin saturation (%) over time in the adjusted population. Figure 2. Respiratory rate (breath per minute) over time in the adjusted population. Figure 3. ROX Index [(SpO2/FiO2) / Respiratory rate] over time in the adjusted population. Figure 4. Probability of being intubated in patients stratified by PaO2/FiO2. Figure 5. Probability of 28-day mortality in patients stratified by PaO2/FiO2.

Objectives To assess modifications in baseline specific IgE- and anti-IgE- and antigen-specific-mediated basophil activation in egg-allergic children. The values were compared before and after the children completed specific oral tolerance induction (SOTI) with egg. Patients and methods We studied 28 egg-allergic children who completed SOTI with egg. The basophil activation test and specific IgE determinations with egg white, ovalbumin, and ovomucoid were performed in all 28 children. Results A decrease in antigen-specific activation with egg white, ovalbumin, and ovomucoid was observed only at the 2 lowest concentrations used (5 and 0.05 ng/mL). Baseline activation was higher in patients with multiple food allergies and in those who developed anaphylaxis during SOTI; this activation decreased in both groups after completion of SOTI. A significant decrease was also observed in specific IgE values for egg white, ovalbumin, and ovomucoid after tolerance induction. Conclusions Food tolerance induction is a specific process for each food that can be mediated by immunologic changes such as a decrease in specific IgE values and in specific and spontaneous basophil activation.

Recientemente se ha descrito la utilidad de los anhídridos de difructosa (DFAs) como buenos marcadores para la detección de adulteraciones de miel con jarabes de alto contenido en fructosa (HFCS) y de azúcar invertido (IS). Sin embargo, para la detección de estos compuestos es necesario realizar un fraccionamiento previo de los carbohidratos de estas muestras, dado que la alta concentración de monosacáridos (glucosa y fructosa) presentes en la miel dificulta el análisis de los compuestos minoritarios. Por tanto, el objetivo de este trabajo se basó en la comparación de un tratamiento con levaduras propuesto con anterioridad y un fraccionamiento con carbónactivo para eliminar la fracción de monosacáridos,concentrando así los compuestos minoritarios de la miely en concreto los DFAs presentes en muestras adulteradas.Se llevó a cabo la optimización de dichos métodos para conseguir el mayor enriquecimiento en DFAs comprobándose que ambos son útiles para la detección de adulteraciones de miel de hasta un 5% de HFCS e IS.