1. Evaluation of commonly used ectoderm markers in iPSC trilineage differentiation
- Author
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Kuang, Yu-Lin, Munoz, Antonio, Nalula, Gilbert, Santostefano, Katherine E, Sanghez, Valentina, Sanchez, Gabriela, Terada, Naohiro, Mattis, Aras N, Iacovino, Michelina, Iribarren, Carlos, Krauss, Ronald M, and Medina, Marisa W
- Subjects
Medical Biotechnology ,Biomedical and Clinical Sciences ,Regenerative Medicine ,Stem Cell Research - Induced Pluripotent Stem Cell ,Stem Cell Research ,Stem Cell Research - Induced Pluripotent Stem Cell - Human ,Generic health relevance ,Good Health and Well Being ,Antigens ,Differentiation ,Biomarkers ,Cell Differentiation ,Cells ,Cultured ,Ectoderm ,Humans ,Induced Pluripotent Stem Cells ,Leukocytes ,Mononuclear ,Human induced pluripotent stem cell ,Trilineage differentiation ,Ectoderm marker ,NESTIN ,PAX6 ,Biological Sciences ,Medical and Health Sciences ,Developmental Biology ,Genetics ,Medical biotechnology ,Oncology and carcinogenesis - Abstract
Patient-derived induced pluripotent stem cells (iPSCs) have become a promising resource for exploring genetics of complex diseases, discovering new drugs, and advancing regenerative medicine. Increasingly, laboratories are creating their own banks of iPSCs derived from diverse donors. However, there are not yet standardized guidelines for qualifying these cell lines, i.e., distinguishing between bona fide human iPSCs, somatic cells, and imperfectly reprogrammed cells. Here, we report the establishment of a panel of 30 iPSCs from CD34+ peripheral blood mononuclear cells, of which 10 were further differentiated in vitro into all three germ layers. We characterized these different cell types with commonly used pluripotent and lineage specific markers, and showed that NES, TUBB3, and OTX2 cannot be reliably used as ectoderm differentiation markers. Our work highlights the importance of marker selection in iPSC authentication, and the need for the field to establish definitive standard assays.
- Published
- 2019