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2. Effect of Ferulic Acid Loaded in Nanoparticle on Tissue Transglutaminase Expression Levels in Human Glioblastoma Cell Line.

Author

Dell'Albani, Paola, Carbone, Claudia, Sposito, Giovanni, Spatuzza, Michela, Chiacchio, Maria Assunta, Grasso, Rosaria, Legnani, Laura, Santonocito, Debora, Puglia, Carmelo, Parenti, Rosalba, Puglisi, Giovanni, and Campisi, Agatina

Subjects
FERULIC acid, CELL cycle, GLIOBLASTOMA multiforme, GLIOMAS, CELL lines
Abstract

Glioblastoma (GBM) is one of the most aggressive cancers, characterized by a decrease in antioxidant levels. Evidence has demonstrated that ferulic acid (FA), a natural antioxidant particularly abundant in vegetables and fruits, could be a promising candidate for GBM treatment. Since FA shows a high instability that compromises its therapeutic application, it has been encapsulated into Nanostructured Lipid Carriers (NLCs) to improve its bioavailability in the brain. It has been demonstrated that tissue transglutaminase (TG2) is a multi-functional protein implicated in many physiological and pathological processes, including cancer. TG2 is also involved in GBM correlated with metastasis formation and drug resistance. Therefore, the evaluation of TG2 expression levels and its cellular localization are important to assess the anti-cancer effect of FA against GBM cancer. Our results have demonstrated that treatment with free FA and FA-NLCs in the U87-MG cancer cell line differently modified TG2 localization and expression levels. In the cells treated with free FA, TG2 appeared expressed both in the cytosol and in the nucleus, while the treatment with FA-NLCs showed that the protein is exclusively localized in the cytosol, exerting its pro-apoptotic effect. Therefore, our data suggest that FA loaded in NLCs could represent a promising natural agent for supplementing the current anti-cancer drugs used for the treatment of GBM. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Effects of Lipid Phase Content on the Technological and Sensory Properties of O/W Emulsions Containing Bemotrizinol-Loaded Nanostructured Lipid Carriers.

Author

Santonocito, Debora, Puglia, Carmelo, and Montenegro, Lucia

Subjects
EMULSIONS, LIPIDS, NANOCARRIERS
Abstract

Different lipid phase ratios (12, 14, and 16% w/w) were assessed for their ability to affect the technological and sensory properties of O/W emulsions in which bemotrizinol (BMTZ), a broad-spectrum sunscreen agent, was incorporated free or loaded into nanostructured lipid nanocarriers (NLC) to reduce its release from the vehicle and, hence, its skin permeation. The following technological properties were evaluated in vitro: spreadability, viscosity, pH, occlusion factor, BMTZ release, and sun protection factor (SPF). Sensory attributes were assessed by panelists in three different phases: before/during pick-up, rub-in, and after application. Raising the lipid phase ratio led to an increase in viscosity (from 8017 ± 143 cPs to 16,444 ± 770 cPs) and to a corresponding decrease in spreadability (from 9.35 ± 0.21 cm to 7.50 ± 0.10 cm), while the incorporation of BMTZ-loaded NLC determined a decrease in the occlusion factor (from 47.75 ± 1.16 to 25.91 ± 1.57) and an increase in SPF (from 6% for formulations containing 12% lipid phase to 15% for formulations containing 16% lipid phase). No BMTZ release was observed from all emulsions. Sensory attributes were mainly affected by the lipid phase ratio. These results suggest that the lipid phase ratio and BMTZ incorporation into NLC could contribute to determining the technological and sensory properties of O/W emulsions. [ABSTRACT FROM AUTHOR]

Published
2024
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4. Correction: Santonocito et al. Astaxanthin-Loaded Stealth Lipid Nanoparticles (AST-SSLN) as Potential Carriers for the Treatment of Alzheimer’s Disease: Formulation Development and Optimization. Nanomaterials 2021, 11, 391

Author

Santonocito, Debora, primary, Raciti, Giuseppina, additional, Campisi, Agata, additional, Sposito, Giovanni, additional, Panico, Annamaria, additional, Siciliano, Edy Angela, additional, Sarpietro, Maria Grazia, additional, Damiani, Elisabetta, additional, and Puglia, Carmelo, additional

Published
2023
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5. Lipid Nanoparticles Loading Steroidal Alkaloids of Tomatoes Affect Neuroblastoma Cell Viability in an In Vitro Model

Author

Santonocito, Debora, primary, Campisi, Agatina, additional, Pellitteri, Rosalia, additional, Sposito, Giovanni, additional, Basilicata, Manuela Giovanna, additional, Aquino, Giovanna, additional, Pepe, Giacomo, additional, Sarpietro, Maria Grazia, additional, Pittalà, Maria Gaetana Giovanna, additional, Schoubben, Aurelie, additional, Pignatello, Rosario, additional, and Puglia, Carmelo, additional

Published
2023
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8. Steroidal Alkaloids from Food Waste of Tomato Processing Inhibit Neuroblastoma Cell Viability.

Author

Santonocito, Debora, Delli Carri, Matteo, Campisi, Agatina, Sposito, Giovanni, Pellitteri, Rosalia, Raciti, Giuseppina, Cardullo, Nunzio, Aquino, Giovanna, Basilicata, Manuela Giovanna, Pepe, Giacomo, Pignatello, Rosario, and Puglia, Carmelo

Subjects
*LYCOPENE, *STEROIDAL alkaloids, *FOOD waste, *LIQUID chromatography-mass spectrometry, *CELL survival, *BOTANICAL chemistry, *TOMATOES, *MEMBRANE lipids
Abstract

Nowadays, there is considerable attention toward the use of food waste from food processing as possible sources of compounds with health properties, such as anticancer activity. An example is tomato processing, which is responsible for generating a remarkable amount of waste (leaves, peel, seeds). Therefore, our goal was to evaluate the potential anticancer property of tomato extracts, in particular "Datterino" tomato (DT) and "Piccadilly" tomato (PT), and to study their phytochemical composition. Liquid chromatography with tandem mass spectrometry (LC/MS-MS) results showed that these extracts are rich in alkaloids, flavonoids, fatty acids, lipids, and terpenes. Furthermore, their potential anticancer activity was evaluated in vitro by MTT assay. In particular, the percentage of cell viability was assessed in olfactory ensheathing cells (OECs), a particular glial cell type of the olfactory system, and in SH-SY5Y, a neuroblastoma cell line. All extracts (aqueous and ethanolic) did not lead to any significant change in the percentage of cell viability on OECs when compared with the control. Instead, in SH-SY5Y we observed a significant decrease in the percentage of cell viability, confirming their potential anticancer activity; this was more evident for the ethanolic extracts. In conclusion, tomato leaves extracts could be regarded as a valuable source of bioactive compounds, suitable for various applications in the food, nutraceutical, and pharmaceutical fields. [ABSTRACT FROM AUTHOR]

Published
2023
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12. Formulation of Solid Lipid Nanoparticles Loaded with Nociceptin/Orphanin FQ (N/OFQ) and Characterization in a Murine Model of Airway Hyperresponsiveness

Author

Mirra, Davida, primary, Spaziano, Giuseppe, additional, Esposito, Renata, additional, Santonocito, Debora, additional, Filosa, Rosanna, additional, Roviezzo, Fiorentina, additional, Malgieri, Gaetano, additional, D’Abrosca, Gianluca, additional, Iovino, Pasquale, additional, Gallelli, Luca, additional, Fattorusso, Roberto, additional, Puglia, Carmelo, additional, and D’Agostino, Bruno, additional

Published
2022
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13. Effect of Unloaded and Curcumin-Loaded Solid Lipid Nanoparticles on Tissue Transglutaminase Isoforms Expression Levels in an Experimental Model of Alzheimer’s Disease

Author

Campisi, Agatina, primary, Sposito, Giovanni, additional, Pellitteri, Rosalia, additional, Santonocito, Debora, additional, Bisicchia, Julia, additional, Raciti, Giuseppina, additional, Russo, Cristina, additional, Nardiello, Pamela, additional, Pignatello, Rosario, additional, Casamenti, Fiorella, additional, and Puglia, Carmelo, additional

Published
2022
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18. Lipid Nanoparticles Traverse Non-Corneal Path to Reach the Posterior Eye Segment: In Vivo Evidence

Author

Puglia, Carmelo, primary, Santonocito, Debora, additional, Romeo, Giuseppe, additional, Intagliata, Sebastiano, additional, Romano, Giovanni Luca, additional, Strettoi, Enrica, additional, Novelli, Elena, additional, Ostacolo, Carmine, additional, Campiglia, Pietro, additional, Sommella, Eduardo Maria, additional, Pignatello, Rosario, additional, and Bucolo, Claudio, additional

Published
2021
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20. Astaxanthin-Loaded Stealth Lipid Nanoparticles (AST-SSLN) as Potential Carriers for the Treatment of Alzheimer’s Disease: Formulation Development and Optimization

Author

Santonocito, Debora, primary, Raciti, Giuseppina, additional, Campisi, Agata, additional, Sposito, Giovanni, additional, Panico, Annamaria, additional, Siciliano, Edy Angela, additional, Sarpietro, Maria Grazia, additional, Damiani, Elisabetta, additional, and Puglia, Carmelo, additional

Published
2021
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26. Formulation and Characterization of Solid Lipid Nanoparticles Loading RF22-c, a Potent and Selective 5-LO Inhibitor, in a Monocrotaline-Induced Model of Pulmonary Hypertension

Author

Liparulo, Angela, primary, Esposito, Renata, additional, Santonocito, Debora, additional, Muñoz-Ramírez, Alejandra, additional, Spaziano, Giuseppe, additional, Bruno, Ferdinando, additional, Xiao, Jianbo, additional, Puglia, Carmelo, additional, Filosa, Rosanna, additional, Berrino, Liberato, additional, and D'Agostino, Bruno, additional

Published
2020
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32. Innovative Nanoparticles Enhance N-Palmitoylethanolamide Intraocular Delivery

Author

Puglia, Carmelo, primary, Blasi, Paolo, additional, Ostacolo, Carmine, additional, Sommella, Eduardo, additional, Bucolo, Claudio, additional, Platania, Chiara B. M., additional, Romano, Giovanni L., additional, Geraci, Federica, additional, Drago, Filippo, additional, Santonocito, Debora, additional, Albertini, Barbara, additional, Campiglia, Pietro, additional, Puglisi, Giovanni, additional, and Pignatello, Rosario, additional

Published
2018
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33. Nanotechnological Approach to Increase the Antioxidant and Cytotoxic Efficacy of Crocin and Crocetin.

Author

Puglia, Carmelo, Santonocito, Debora, Musumeci, Teresa, Cardile, Venera, Graziano, Adriana Carol Eleonora, Salerno, Loredana, Raciti, Giuseppina, Crascì, Lucia, Panico, Anna Maria, and Puglisi, Giovanni

Subjects
*CALORIMETRY, *CAROTENOIDS, *CELL death, *CELL surface antigens, *DIFFUSION, *DRUG delivery systems, *IMMUNODIAGNOSIS, *MOLECULAR structure, *NANOPARTICLES, *SPECTRUM analysis, *OXIDATIVE stress
Abstract

Crocin and crocetin are two interesting constituents of saffron (Crocus sativus) that possess important biological activities. Their use as therapeutic agents is strongly compromised by a scarce stability, poor absorption, and low bioavailability. Therefore, to improve these unfavorable features, the aim of the present work has been to apply a nanotechnological approach based on the formulation of solid lipid nanoparticles containing crocin and crocetin. Solid lipid nanoparticles were formulated according to crocin and crocetin chemical properties, using a variation of the quasi-emulsion solvent diffusion method to formulate crocin-solid lipid nanoparticles, while crocetin-solid lipid nanoparticles were prepared following the solvent diffusion method. Morphology and dimensional distribution of solid lipid nanoparticles have been characterized by differential scanning calorimetry and photon correlation spectroscopy, respectively, while the effect of drug incorporation versus time has been studied by Turbiscan technology. In order to verify the role of the nanotechnological approach on the biological activities of crocin and crocetin, the antioxidant and antiproliferative effects of these carotenoids once incorporated in lipid nanoparticles have been evaluated. For this aim, the oxygen radical absorbance capacity assay and the MTT test were used, respectively. The results pointed out the formulation of nanometric dispersions endowed with high homogeneity and stability, with an encapsulation efficiency ranging from 80 (crocetin-solid lipid nanoparticles) to 94% (crocin-crocetin). The oxygen radical absorbance capacity assay evidenced an interesting and prolonged antioxidant activity of crocin and crocetin once encapsulated in solid lipid nanoparticles, while the nanoencapsulation strategy showed a different mechanism in ameliorating the cytotoxic effect of these two substances. [ABSTRACT FROM AUTHOR]

Published
2019
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34. Lipid Nanoparticles Traverse Non-Corneal Path to Reach the Posterior Eye Segment: In Vivo Evidence

Author

Debora Santonocito, Carmine Ostacolo, Rosario Pignatello, Enrica Strettoi, Elena Novelli, Sebastiano Intagliata, Giovanni Luca Romano, Claudio Bucolo, Giuseppe Romeo, Pietro Campiglia, Carmelo Puglia, Eduardo Sommella, Puglia, Carmelo, Santonocito, Debora, Romeo, Giuseppe, Intagliata, Sebastiano, Romano, Giovanni Luca, Strettoi, Enrica, Novelli, Elena, Ostacolo, Carmine, Campiglia, Pietro, Sommella, Eduardo Maria, Pignatello, Rosario, and Bucolo, Claudio

Subjects
Posterior Eye Segment, Male, retina, Pharmaceutical Science, Excipient, Organic chemistry, 02 engineering and technology, 030226 pharmacology & pharmacy, ocular drug delivery, fluorescence microscopy, Article, Analytical Chemistry, Cornea, 03 medical and health sciences, Mice, 0302 clinical medicine, QD241-441, Drug Delivery Systems, In vivo, Drug Discovery, medicine, Zeta potential, Animals, nanomedicine, fluorescent nanoparticle, Spiro Compounds, Physical and Theoretical Chemistry, Active ingredient, Drug Carriers, Chemistry, 021001 nanoscience & nanotechnology, Fluorescence, Lipids, Mice, Inbred C57BL, medicine.anatomical_structure, Chemistry (miscellaneous), Molecular Medicine, ocular drug delivery, nanomedicine, fluorescent nanoparticle, fluorescence microscopy, retina, Nanoparticles, Rabbits, Nanocarriers, 0210 nano-technology, medicine.drug, Biomedical engineering
Abstract

Lipid-based nanocarriers (LNs) have made it possible to prolong corneal residence time and improve the ocular bioavailability of ophthalmic drugs. In order to investigate how the LNs interact with the ocular mucosa and reach the posterior eye segment, we have formulated lipid nanocarriers that were designed to bear a traceable fluorescent probe in the present work. The chosen fluorescent probe was obtained by a conjugation reaction between fluoresceinamine and the solid lipid excipient stearic acid, forming a chemically synthesized adduct (ODAF, N-(3′,6′-dihydroxy-3-oxospiro [isobenzofuran-1(3H),9′-[9H] xanthen]-5-yl)-octadecanamide). The novel formulation (LN-ODAF) has been formulated and characterized in terms of its technological parameters (polydispersity index, mean particle size and zeta potential), while an in vivo study was carried out to assess the ability of LN-ODAF to diffuse through different ocular compartments. LN-ODAF were in nanometric range (112.7 nm ± 0.4), showing a good homogeneity and long-term stability. A TEM (transmission electron microscopy) study corroborated these results of characterization. In vivo results pointed out that after ocular instillation, LN ODAF were concentrated in the cornea (two hours), while at a longer time (from the second hour to the eighth hour), the fluorescent signals extended gradually towards the back of the eye. From the results obtained, LN-ODAF demonstrated a potential use of lipid-based nanoparticles as efficient carriers of an active pharmaceutical ingredient (API) involved in the management of retinal diseases.

Published
2021

35. Ocular Formulation Based on Palmitoylethanolamide-Loaded Nanostructured Lipid Carriers: Technological and Pharmacological Profile

Author

Filippo Drago, Eduardo Sommella, Claudio Bucolo, Rosario Pignatello, Debora Santonocito, Pietro Campiglia, Carmelo Puglia, Carmine Ostacolo, Claudia Carbone, Puglia, Carmelo, Santonocito, Debora, Ostacolo, Carmine, Maria Sommella, Eduardo, Campiglia, Pietro, Carbone, Claudia, Drago, Filippo, Pignatello, Rosario, and Bucolo, Claudio

Subjects
General Chemical Engineering, nanostructured lipid carriers, 02 engineering and technology, nanostructured lipid carrier, 01 natural sciences, ocular drug delivery, Article, lcsh:Chemistry, chemistry.chemical_compound, Turbiscan technology, 0103 physical sciences, medicine, General Materials Science, palmitoylethanolamide, Retinal Tumor, Palmitoylethanolamide, Retina, 010304 chemical physics, Natural compound, food and beverages, Retinal, Diabetic retinopathy, 021001 nanoscience & nanotechnology, medicine.disease, diabetic retinopathy, Nanostructured lipid carriers, Ocular drug delivery, medicine.anatomical_structure, lcsh:QD1-999, chemistry, Drug delivery, Physical stability, 0210 nano-technology, Biomedical engineering
Abstract

The present work was aimed for the preparation of a stable nanostructured lipid carrier (NLC) system for the delivery of N-palmitoylethanolamide (PEA) to the back of the eye. PEA is an interesting natural compound showing anti-inflammatory and neuroprotective activities. The limits of PEA (poor solubility and high instability) justify its nanoencapsulation into drug delivery systems. Two different well-known techniques were compared to formulate NLC: the high shear homogenization technique (HSH) and the method based on a combination of HSH technique and ultrasonication (HSH/US). Nanoparticles were evaluated in relation to mean size, homogeneity, surface charge, and physical stability by Turbiscan technology. Retinal distribution of PEA was carried out in a rat eye after single instillation of PEA-NLC ophthalmic formulation. The novel formulation delivered remarkable levels of PEA to the retina. Lastly, topical administration of PEA-NLC ophthalmic formulation was able to significantly inhibits retinal tumor necrosis factor-&alpha, (TNF-&alpha, ) levels in streptozotocin-induced diabetic rats. The present findings suggest that the novel ophthalmic formulation may be useful for the treatment of retinal diseases such as diabetic retinopathy. Clinical studies are in progress to evaluate this possibility.

Published
2020

36. Innovative Nanoparticles Enhance N-Palmitoylethanolamide Intraocular Delivery

Author

Carmelo Puglia, Paolo Blasi, Carmine Ostacolo, Eduardo Sommella, Claudio Bucolo, Chiara B. M. Platania, Giovanni L. Romano, Federica Geraci, Filippo Drago, Debora Santonocito, Barbara Albertini, Pietro Campiglia, Giovanni Puglisi, Rosario Pignatello, Puglia, Carmelo, Blasi, Paolo, Ostacolo, Carmine, Sommella, Eduardo, Bucolo, Claudio, Platania, Chiara B. M., Romano, Giovanni L., Geraci, Federica, Drago, Filippo, Santonocito, Debora, Albertini, Barbara, Campiglia, Pietro, Puglisi, Giovanni, Pignatello, Rosario, Puglia C., Blasi P., Ostacolo C., Sommella E., Bucolo C., Platania C.B., Romano G.L., Geraci F., Drago F., Santonocito D., Albertini B., Campiglia P., Puglisi G., and Pignatello R.

Subjects
0301 basic medicine, genetic structures, nanostructured lipid carriers, Cmax, Nanoparticle, Nanostructured lipid carrier, 02 engineering and technology, ocular drug delivery, 03 medical and health sciences, chemistry.chemical_compound, Pharmacokinetics, Liquid chromatography–mass spectrometry, medicine, Pharmacology (medical), palmitoylethanolamide, Diabetic retinopathy, Nanostructured lipid carriers, Ocular drug delivery, Palmitoylethanolamide, Retinal diseases, Pharmacology, Original Research, Chromatography, Chemistry, lcsh:RM1-950, food and beverages, Retinal, 021001 nanoscience & nanotechnology, medicine.disease, eye diseases, Clinical Practice, diabetic retinopathy, 030104 developmental biology, retinal diseases, lcsh:Therapeutics. Pharmacology, palmitoylethanolamide, nanostructured lipid carriers, ocular drug delivery, retinal diseases, diabetic retinopathy, sense organs, 0210 nano-technology
Abstract

Nanostructured lipid carriers (NLCs) loaded with palmitoylethanolamide (PEA) were formulated with the aim to enhance ocular bioavailability of PEA, particularly to the back of the eye. Technological characterization (e.g., size, charge) of NLC loaded with PEA formulation (PEA-NLC) was performed, and NLC morphology was characterized by electron microscopy. Ocular pharmacokinetic study, after topical administration of the formulation, was carried out in rabbit eye. Ultra-high performance liquid chromatography tandem mass spectrometry analysis was carried out to detect PEA levels in ocular tissues. Finally, the ocular tolerability of PEA-NLC formulation was assessed in rabbit eye. The novel formulation significantly increased PEA levels in ocular tissues compared to PEA suspension. Vitreous and retinal levels of PEA were significantly higher in the group treated with PEA-NLC formulation versus PEA suspension (PEA-NLC C-max 5919 +/- 541 pmol/g and 315 +/- 70 pmol/g in vitreous and retina, respectively). The PEA-NLC formulation was characterized by high stability and robust ocular bioavailability. Therefore, this innovative formulation may be useful in clinical practice to manage retinal diseases.

Published
2018

37. Mediterranean Food By-products as a Valuable Source of Bioactive Compounds with Health Properties.

Author

Santonocito D, Montenegro L, and Puglia C

Abstract

Recently, considerable attention has been focused on the recovery and re-use of waste plant matrices as promising sources of bioactive compounds with health effects. As the Mediterranean diet involves the consumption of great amounts of fruits and vegetables, large quantities of agro-food by-products are generated, causing economic and environmental problems. Such by-products contain a great variety of bioactive compounds whose potential health benefits include anti-inflammatory, antioxidant, anti-- cancer, antimicrobial, hypoglycemic, antidepressant, cardio- and neuro-protective activities. Therefore, in this review, by-products from the most common fruits and vegetables processed in the Mediterranean area, such as tomato, olive, citrus fruit, almond, pomegranate, carob, date, and grape, were taken into account, pointing out the content of bioactive ingredients in extracts obtained from different parts of plants, fruits, and vegetables. Furthermore, studies performed to assess the beneficial effects of extracts obtained from Mediterranean agro-food by-products were reviewed, highlighting the potential benefits of waste plant matrices re-usage in the pharmaceutical, nutraceutical, and cosmetic fields., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

Published
2024
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