13 results on '"Santi-Neto D"'
Search Results
2. Physiological Stimulus for the Synthesis of Basement Membrane Proteins Leading to Its Reconstruction.
- Author
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Pereira de Godoy JM, Guerreiro Godoy MF, Pereira de Godoy AC, and Santi Neto D
- Abstract
The aim of the present study was to report the remodeling of the basement membrane through physiological stimulus during the treatment of fibrosis in a lower limb with lymphedema. A clinical trial was conducted involving the evaluation of the basement membrane in skin biopsies before and after treatment for clinical stage II lower limb lymphedema using the Godoy method for the reversal of lymphedema and skin fibrosis. The samples were stained with Gomori's reticulin stain and evaluated using Weibel's multipoint morphometric method at the Godoy Clinic. Prior to treatment for lymphedema, rupture and important discontinuity of the basement membrane was found. After treatment, structural continuity and thickness had returned to the regions of previous rupture. The difference was statistically significant (P < 0.05, paired t -test). The present study reports that physiological stimuli targeting the lymphatic system led to the clinical reversal of fibrosis, as well as stimulate the synthesis of extracellular matrix proteins and the reconstruction of the basal lamina of the skin., Competing Interests: The authors declared no conflict of interest., (Copyright 2024, Pereira de Godoy et al.)
- Published
- 2024
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3. Epidemiological and molecular evaluation of BRAF, KRAS, NRAS genes and MSI in the development of colorectal cancer.
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Paula Simedan Vila A, Helena Rodrigues G, Leite Marzochi L, Garcia de Oliveira-Cucolo J, Lívia Silva Galbiatti-Dias A, Felipe Maciel Andrade R, de Santi Neto D, Gomes Netinho J, Castiglioni L, Cristina Pavarino É, and Maria Goloni-Bertollo E
- Subjects
- Humans, Male, Middle Aged, Female, Microsatellite Instability, Proto-Oncogene Proteins p21(ras) genetics, Genes, ras, Mutation, Membrane Proteins genetics, GTP Phosphohydrolases genetics, Proto-Oncogene Proteins B-raf genetics, Colorectal Neoplasms epidemiology, Colorectal Neoplasms genetics
- Abstract
Background and Objectives: KRAS, NRAS, BRAF mutations and microsatellite instability (MSI) can be associated with Colorectal Cancer (CRC) development., Material and Methods: We evaluated 828 medical records of CRC patients from a school hospital from January/2016 to December/2020. Variables such as age, gender, ethnicity, literacy level, smoking, alcoholism, primary anatomical site, tumor staging, presence of BRAFV600E, KRAS, NRAS mutations and MSI , survival and metastasis were identified. The statistical analyses were performed (p < 0.05 was considered significant)., Results: There was a predominance of males (51.93%), whites (90.70%), low education (72.34%), smokers (73.79%), and non-alcoholics (79.10%). Rectum was the most affected site (42.14%), advanced tumor stage was most prevalent (62.07%), and metastasis occurred in (64.61%). Of the enrolled patients; 204 were investigated for BRAF mutation and detected in (2.94%); 216 for KRAS gene and detected in (26.08%); 210 for NRAS gene, and detected in (25.36%); 370 for MSI and detected in (44.68%). A significant association of CRC with NRAS mutation and alcohol habit (p = 0.043) was observed. The presence of MSI was associated with primary site proximal colon (p < 0.000), distal colon (p = 0.001) and rectum (p = 0.010)., Conclusion: Patients with CRC are male, over 64 years old, white, with low education, smokers and non-alcoholics. The most affected primary site is rectum in advanced stage with metastasis. CRC is associated with NRAS mutation and alcohol habit, there is increased risk for primary site of proximal colon and MSI; decreased risk for distal colon and rectum in the presence of MSI., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)
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- 2023
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4. Synthesis and Physiological Remodeling of CD34 Cells in the Skin following the Reversal of Fibrosis through Intensive Treatment for Lower Limb Lymphedema: A Case Report.
- Author
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Pereira de Godoy JM, Pereira de Godoy AC, Guerreiro Godoy MF, and de Santi Neto D
- Abstract
A novel type of cell underwent identification between 2005 and 2008 and was denominated the "telocyte" in 2010. In 2012, transmission electron microscopy revealed the presence of telocytes in the dermis. The aim of the present study was to report important changes in immunostained CD34 cells following the treatment of lower limb lymphedema using a specific lymphatic therapy technique. A clinical trial involving the evaluation of changes in immunostained CD34 cells in the epidermis and dermis (10 randomly selected histological fields) of a patient before and after intensive treatment for clinical stage II lymphedema was conducted using the Godoy Method, which was adapted to the treatment of skin fibrosis. The evaluation involved the use of the Weibel multi-point morphometric method. Comparisons were performed using the t -test with a 95% significance level. An important increase in CD34 cells was found with redistribution occurring following treatment. The treatment of primary lymphedema of the lower limbs resulted in the clinical reversal of fibrosis and an increase in the number of immunomarked CD34 cells.
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- 2023
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5. Stimulation of Synthesis and Lysis of Extracellular Matrix Proteins in Fibrosis Associated with Lymphedema.
- Author
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Pereira de Godoy JM, Guerreiro Godoy MF, Pereira de Godoy HJ, and De Santi Neto D
- Abstract
Background : Fibrotic diseases pose a problem for overall health due to their chronic, progressive nature; the lack of a cure; and the fact that such conditions are largely refractory to current medical and surgical treatment practices. Objective : The aim of the present study was to report the physiological stimulation of synthesis and lysis of extracellular matrix proteins during the treatment of primary lymphedema. Material and Methods : A clinical trial was conducted involving the analysis of changes in type I and III collagen fibers and elastic fibers as well as the thickness of the epidermis and dermis in 10 histological fields. Samples were taken from the skin before and after intensive treatment using the Godoy Method
® and adapted to the treatment of fibrosis in a patient with a clinical diagnosis of lower limb lymphedema. Slides were stained with orcein, hematoxylin and eosin, picrosirius red, and Gomori's reticulin stains. Weibel's multipoint method was used for the morphometric evaluation. The data were compared using the t -test with a 95% confidence interval. Results : Significant changes were detected in all aspects of interest (thickness of the epidermis and dermis, type I and III collagen fibers, and elastic fibers). Conclusion : The present findings demonstrate the physiological stimulation of synthesis and lysis of the main components of an extracellular matrix, such as type I and III collagen fibers and elastic fibers, as well as a reduction in the thickness of the epidermis and dermis in cases of fibrosis through adequate stimulation of the lymphatic system.- Published
- 2021
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6. VEGFA and NFE2L2 Gene Expression and Regulation by MicroRNAs in Thyroid Papillary Cancer and Colloid Goiter.
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Stuchi LP, Castanhole-Nunes MMU, Maniezzo-Stuchi N, Biselli-Chicote PM, Henrique T, Padovani Neto JA, de-Santi Neto D, Girol AP, Pavarino EC, and Goloni-Bertollo EM
- Subjects
- Apoptosis, Biomarkers, Tumor genetics, Case-Control Studies, Cell Proliferation, Female, Gene Expression Regulation, Neoplastic, Goiter, Nodular genetics, Goiter, Nodular metabolism, Humans, Male, Middle Aged, NF-E2-Related Factor 2 genetics, Prognosis, Thyroid Cancer, Papillary genetics, Thyroid Cancer, Papillary metabolism, Thyroid Neoplasms genetics, Thyroid Neoplasms metabolism, Thyroid Neoplasms pathology, Tumor Cells, Cultured, Vascular Endothelial Growth Factor A genetics, Biomarkers, Tumor metabolism, Goiter, Nodular pathology, MicroRNAs genetics, NF-E2-Related Factor 2 metabolism, Thyroid Cancer, Papillary pathology, Vascular Endothelial Growth Factor A metabolism
- Abstract
Deregulation of VEGFA (Vascular Endothelial Growth Factor A) and NFE2L2 (Nuclear Factor (Erythroid-derived 2)-Like 2), involved in angiogenesis and oxidative stress, can lead to thyroid cancer progression. MiR-17-5p and miR-612 are possible regulators of these genes and may promote thyroid disorders. In order to evaluate the involvement of VEGFA, NFE2L2, hsa-miR-17-5p, and hsa-miR-612 in thyroid pathology, we examined tissue samples from colloid goiter, papillary thyroid cancer (PTC), and a normal thyroid. We found higher levels of VEGFA and NFE2L2 transcripts and the VEGFA protein in goiter and PTC samples than in normal tissue. In the goiter, miR-612 and miR-17-5p levels were lower than those in PTC. Tumors, despite showing lower VEGFA mRNA expression, presented higher VEGFA protein levels compared to goiter tissue. In addition, NRF2 (Nuclear Related Transcription Factor 2) protein levels in tumors were higher than those in goiter and normal tissues. Inhibition of miR-17-5p resulted in reduced NFE2L2 expression. Overall, both transcript and protein levels of NFE2L2 and VEGFA were elevated in PTC and colloid goiter. Hsa-miR-612 showed differential expression in PTC and colloid goiter, while hsa-miR-17-5p showed differential expression only in colloid goiter, suggesting that hsa-miR-17-5p may be a positive regulator of NFE2L2 expression in PTC.
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- 2020
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7. Differential Expression of Prostaglandin I2 Synthase Associated with Arachidonic Acid Pathway in the Oral Squamous Cell Carcinoma.
- Author
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Russo A, Biselli-Chicote PM, Kawasaki-Oyama RS, Castanhole-Nunes MMU, Maniglia JV, de Santi Neto D, Pavarino ÉC, and Goloni-Bertollo EM
- Abstract
Introduction: Differential expression of genes encoding cytochrome P450 (CYP) and other oxygenases enzymes involved in biotransformation mechanisms of endogenous and exogenous compounds can lead to oral tumor development., Objective: We aimed to identify the expression profile of these genes, searching for susceptibility biomarkers in oral squamous cell carcinoma., Patients and Methods: Sixteen oral squamous cell carcinoma samples were included in this study (eight tumor and eight adjacent non-tumor tissues). Gene expression quantification was performed using TaqMan Array Human CYP450 and other Oxygenases 96-well plate (Applied Biosystems) by real time qPCR. Protein quantification was performed by ELISA and IHC methods. Bioinformatics tools were used to find metabolic pathways related to the enzymes encoded by differentially expressed genes. Results. CYP27B1, CYP27A1, CYP2E1, CYP2R1, CYP2J2, CYP2U1, CYP4F12, CYP4X1, CYP4B1, PTGIS, ALOX12, and MAOB genes presented differential expression in the oral tumors. After correction by multiple tests, only the PTGIS (Prostaglandin I2 Synthase) gene presented significant differential expression (P < 0.05). The PTGIS gene and protein were reduced in oral tumors., Conclusion: PTGIS presents downexpression in oral tumors. PTGIS play an important role in the arachidonic acid metabolism. Arachidonic acid and/or metabolites are derived from this pathway, which can influence the regulation of important physiological mechanisms in tumorigenesis process.
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- 2018
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8. Candidate Biomarkers for Oral Squamous Cell Carcinoma: Differential Expression of Oxidative Stress-Related Genes
- Author
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Pedro NF, Biselli JM, Maniglia JV, Santi-Neto D, Pavarino ÉC, Goloni-Bertollo EM, and Biselli-Chicote PM
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- Aged, Carcinoma, Squamous Cell pathology, Case-Control Studies, Female, Follow-Up Studies, Humans, Male, Mouth Neoplasms pathology, Prognosis, Biomarkers, Tumor genetics, Carcinoma, Squamous Cell genetics, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Mouth Neoplasms genetics, Oxidative Stress genetics
- Abstract
Background: Alteration in the biotransformation of exogenous compounds can result in production of reactive oxygen species (ROS), which can predispose cells to malignant transformation in the head and neck. This study aimed to evaluate the expression of genes involved in antioxidant metabolism in the oral squamous cell carcinoma (OSCC). Methods: The expression of eighty-four genes was evaluated in OSCC and non-tumor tissues by quantitative real-time polymerase chain reaction using the TaqMan Gene Expression Array. The biological mechanisms related to the differentially expressed genes were investigated using Gene – NCBI, KEGG, UNIPROT and REACTOME databases. Results: Twenty-one genes encoding enzymes involved in antioxidant metabolism were differentially expressed in the OSCC case. Four genes (ATOX1, PRDX4, PRNP, and SOD2) were up-regulated, and seventeen (ALOX12, CAT, CSDE1, DHCR24, DUOX1, DUOX2, EPHX2, GLRX2, GPX3, GSR, GSTZ1, MGST3, PRDX1, OXR1, OXSR1, SOD1, and SOD3) were down-regulated. We identified 14 possible novel biomarkers for OSCC. The differentially expressed genes appeared related to important biological processes involved in carcinogenesis, such as inflammation, angiogenesis, apoptosis, genomic instability, invasion, survival, and cell proliferation. Conclusions: Our study identified novel biomarkers which might warrant further investigation regarding OSCC pathogenesis since the altered expression in the genes can modulate biological processes related to oxidative stress and predispose cells to malignant transformation in the oral cavity., (Creative Commons Attribution License)
- Published
- 2018
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9. Deregulation of annexin-A1 and galectin-1 expression in precancerous gastric lesions: intestinal metaplasia and gastric ulcer.
- Author
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Rossi AF, Duarte MC, Poltronieri AB, Valsechi MC, Jorge YC, de-Santi Neto D, Rahal P, Oliani SM, and Silva AE
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- Antigens, Bacterial genetics, Bacterial Proteins genetics, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Genotype, Helicobacter pylori genetics, Humans, Immunohistochemistry, Intestinal Mucosa metabolism, Intestines pathology, Metaplasia metabolism, Precancerous Conditions, Risk Factors, Stomach pathology, Annexin A1 metabolism, Galectin 1 metabolism, Gastric Mucosa metabolism, Inflammation metabolism, Stomach Ulcer metabolism
- Abstract
Objective: Annexin-A1 (ANXA1/AnxA1) and galectin-1 (LGALS1/Gal-1) are mediators that play an important role in the inflammatory response and are also associated with carcinogenesis. We investigated mRNA and protein expression in precancerous gastric lesions that participate in the progression cascade to gastric cancer, such as intestinal metaplasia (IM) and gastric ulcer (GU)., Methods: Quantitative real-time PCR (qPCR) and immunohistochemical techniques were used to analyze the relative quantification levels (RQ) of ANXA1 and LGALS1 mRNA and protein expression, respectively., Results: Increased relative expression levels of ANXA1 were found in 100% of cases, both in IM (mean RQ = 6.22 ± 0.06) and in GU (mean RQ = 6.69 ± 0.10). However, the LGALS1 presented basal expression in both groups (IM: mean RQ = 0.35 ± 0.07; GU: mean RQ = 0.69 ± 0.09). Immunohistochemistry revealed significant positive staining for both the AnxA1 and Gal-1 proteins in the epithelial nucleus and cytoplasm as well as in the stroma of the IM and GU groups (P < 0.05) but absence or low immunorectivity in normal mucosa., Conclusion: Our results bring an important contribution by evidencing that both the AnxA1 and Gal-1 anti-inflammatory proteins are deregulated in precancerous gastric lesions, suggesting their involvement in the early stages of gastric carcinogenesis, possibly due to an inflammatory process in the gastric mucosa.
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- 2014
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10. Trypanosoma cruzi infection reactivation manifested by encephalitis in a Chagas heart transplant recipient.
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Bestetti RB, Rubio FG, Ferraz Filho JR, Goes MJ, de Santi Neto D, Akio F, and Villafanha DF
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- Adult, Chagas Disease diagnosis, Chagas Disease parasitology, Diagnosis, Differential, Encephalitis diagnosis, Female, Humans, Chagas Disease complications, Encephalitis complications, Encephalitis parasitology, Heart Transplantation adverse effects, Trypanosoma cruzi pathogenicity
- Published
- 2013
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11. Glutathione transferase pi (GSTpi) expression in breast cancer: an immunohistochemical and molecular study.
- Author
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Jardim BV, Moschetta MG, Gelaleti GB, Leonel C, Regiani VR, de Santi Neto D, Bordin-Junior NA, Perea SA, and Zuccari DA
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- Adult, Aged, Aged, 80 and over, Breast Neoplasms diagnosis, Female, Humans, Immunohistochemistry, Middle Aged, Predictive Value of Tests, Prognosis, Retrospective Studies, Reverse Transcriptase Polymerase Chain Reaction, Survival Rate, Breast Neoplasms genetics, Gene Expression Regulation, Neoplastic, Glutathione S-Transferase pi genetics
- Abstract
Breast cancer is the most frequent cancer in women worldwide. Prognostic markers are important for diagnosis, allowing therapeutic strategies to be defined more efficiently. The expression of the glutathione S-transferase pi isoenzyme (GSTpi) in tumor cells has been evaluated as a predictor of prognosis and in response to cytotoxic treatments. Its immunoexpression was assessed in 63 women diagnosed with invasive ductal carcinoma in a retrospective study. The results were statistically correlated with clinicopathological parameters of patients. The results showed that high GSTpi expression was related to p53-positive tumors, grade III histology, large tumor size and death (p<0.05). The 37 patients who received adjuvant treatment, checked separately, showed high expression of GSTpi in relation to local recurrence, metastasis and death (p<0.05). In addition, high levels of GSTpi expression were significantly associated with a shorter overall survival (p<0.05). To confirm this suspicion, GSTpi gene expression was checked by Real-time PCR in neoplastic mammary cells cultured and subjected to treatment with doxorubicin. Our results suggest that high levels of GSTpi may be related to the development of resistance to chemotherapy in these tumors, the response of these tumors to treatment and the clinical course of the patients involved., (Crown Copyright © 2011. Published by Elsevier GmbH. All rights reserved.)
- Published
- 2012
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12. Correlation between the severity of acute hepatic necrosis induced by acetaminophen and serum aminotransferase levels in fasted and sucrose-fed rats.
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Hessel G, De-Santi-Neto D, and Collares EF
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- Acute Disease, Animals, Fasting, Liver Diseases blood, Liver Diseases pathology, Male, Necrosis, Rats, Rats, Wistar, Sucrose administration & dosage, Acetaminophen adverse effects, Alanine Transaminase blood, Aspartate Aminotransferases blood, Chemical and Drug Induced Liver Injury
- Abstract
The relationship between serum aminotransferase levels and the acute hepatic necrosis induced by acetaminophen was studied in 24 male Wistar rats (220-265 g). The animals were divided into two groups, one of which was fasted for 66 h (group I) while the other was fed only sucrose cubes ad libitum (group II). The animals received I g acetaminophen per kg body weight 42 h after the onset of the experiment. Twenty-four hours later, blood was drawn to measure aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels and the liver was removed for both macro- and microscopic examination. The intensity of the hepatic necrosis was scored according to the extent of the lesion. The hepatic necrosis was more frequent and intense in group I, with the aminotransferase levels being higher in this group (median AST and ALT levels were 3900 IU/l and 2511 IU/l, respectively, for group I and 119 IU/l and 79 IU/l, respectively, for group II). There was a positive correlation (rs) between the intensity of hepatic necrosis assessed microscopically and the levels of AST (group I, rs = 0.83; group II, rs = 0.79) and ALT (group I, rs = 0.58; group II, rs = 0.80). These findings suggest that aminotransferase levels are a reliable indicator of the degree of hepatic necrosis in this model of acetaminophen intoxication.
- Published
- 1996
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