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Your search keyword '"Santachiara, Saverio"' showing total 3 results
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3 results on '"Santachiara, Saverio"'

2. Effects of anti-malarial drugs on MCF-7 and Vero cell replication

Author

Rossi, Tiziana, Coppi, Andrea, Bruni, Elisa, Ruberto, Ippazio Antonio, Santachiara, Saverio, and Baggio, Giosuè Gabriele

Subjects
Dose-Response Relationship, Drug, Malaria, Cancer, MCF/7, VERO, Tumour progression, Antineoplastic Agents, Breast Neoplasms, Cell Growth Processes, Adenocarcinoma, Antimalarials, Inhibitory Concentration 50, Doxorubicin, Quinacrine, Cell Line, Tumor, Chlorocebus aethiops, Animals, Humans, Vero Cells
Abstract

Previous in vivo studies performed in our laboratories demonstrated that anti-malarial drugs may or enhance or slow down Ehrlich's ascites tumour progression in infected mice. In the light of these observations, an in vitro study was undertaken to assess the response of human tumour cells to various anti-malarial drugs and consequently the safety of the anti-malarial therapy.MCF-7 cells and Vero cells (control line) were cultured in Eagle's minimum Essential medium (EMEM) and then subjected to graded concentrations of different anti-malarial drugs. Trypan-blue exclusion, MTT and Western blotting tests were performed.The findings showed that pyrimethamine (12.5 mg/L), chloroquine (12.5 mg/L) and primaquine (1.56 mg/L) stimulated MCF-7 cell growth. The proliferative effect was inhibited by doxorubicin only in cultures treated with chloroquine and primaquine. These results might indicate that some anti-malarial drugs have a worrying tumour-promoting effect which should not be underestimated when undertaking anti-malarial prophylactic measures.

Published
2007

3. Selectivity of action of glycyrrhizin derivatives on the growth of MCF-7 and HEP-2 cells.

Author

Rossi T, Castelli M, Zandomeneghi G, Ruberto A, Benassi L, Magnoni C, Santachiara S, and Baggio G

Subjects
Animals, Breast Neoplasms pathology, Cell Division drug effects, Cell Line, Tumor, Chlorocebus aethiops, Drug Screening Assays, Antitumor, Humans, In Situ Nick-End Labeling, Laryngeal Neoplasms pathology, Vero Cells, Breast Neoplasms drug therapy, Glycyrrhetinic Acid pharmacology, Glycyrrhizic Acid pharmacology, Laryngeal Neoplasms drug therapy
Abstract

Since 1986, there have been reports in the literature that glycyrrhizin (GL), 18 alpha-glycyrrhetinic acid (18 alpha-GA) and 18 beta-glycyrrhetinic acid (18 beta-GA) can inhibit the growth of some murine tumours. Recently, testing the activity of GL and 18 alpha-GA on human tumour cells, we observed that both substances are good anti-proliferative agents, especially on those cells whose replication rate is slow. In the present study, the MTT test was performed on MCF-7, Hep-2 and VERO cells treated with increasing concentrations of GL and of its derivatives. Results showed that the substances do not have a toxic effect on VERO cells and that their anti-proliferative effect is evident only on the cell line (MCF-7) that evolves slowly. Finally, the TUNEL test revealed the presence of apoptosis in MCF-7 cells treated with dosages of GL and 18 alpha-GA, thereby confirming that these natural phytoestrogens could be considered as interesting new agents for cancer therapy.

Published
2003

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