35 results on '"Sanhua Li"'
Search Results
2. Six bufadienolides derivatives are the main active substance against human colorectal cancer HCT116 cells of Huachansu injection
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Feng Pan, Keyu Lu, Zeli Chun, Nan Yang, Lingjie Meng, Sanhua Li, and Yun Liu
- Subjects
Huachansu injection ,Bufadienolides derivative ,Colorectal cancer ,Material basis ,UPLC-MS/MS ,HPLC-DAD ,Other systems of medicine ,RZ201-999 ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: Huachansu injection (HCS) is an aqueous extract preparation of Traditional Chinese Medicine (TCM) toad skin (Bufo melanosticus or B. bufo gargarizans). As a national second-class and self-developed TCM preparation, it is widely used to treat human colorectal cancer (CRC), yet its substantial toxic side effects, such as vascular irritation reaction, drug fever, and allergic reactions, pose a considerable challenge during clinical use. In-depth research on the material basis of HCS' traditional efficacy is essential for developing more effective and less toxic medicine. Identifying the main components responsible for the efficacy of HCS is crucial in determining its material basis and explaining any adverse reactions. Purpose: The purpose of this study is to clarify the main active components of HCS as a growth inhibitory agent against CRC HCT116 cells and to further understand the material basis of its anti-CRC properties. Methods: Sulforhodamine B (SRB) assay was used to screen the compound with strong inhibitory activity against HCT116 cells from 44 compounds isolated from aqueous extracts of toad skin (B. melanosticus). The screened compounds as standard and MeOH extracts from HCS were determined by HPLC coupled with triple quadrupole mass spectrometry. The partial MeOH extract was subjected to HPLC system using solvents in a gradient of increasing polarity, yielding 12 fractions in total (HCS1 to HCS12). Besides, the screened active compounds were knocked out from HCS to obtain a fraction as a negative control, and the knocked-out components were collected as one fraction using semi-preparative HPLC. Those fractions were submitted to HPLC-MS/MS or HPLC-DAD system to determine the presence or absence of the screened compounds. In addition, cytotoxicity of those prepared fractions against CRC HCT116 cells was evaluated to determine the effect of the screened compounds in the anti-CRC of HCS. Results: Six compounds, namely arenobufagin, telocinobufagin, gamabufalin, hellebrigenin, 19-hydroxylbufagin and argentinogenin, were screened for their inhibitory activity (with an IC50 value of less than 400 nM), and were present in HCS at a total-mass-percentage of 1.35×10−3 % in HCS MeOH extract. In addition, those 6 compounds were mainly distributed in HCS8, which also showed the strongest inhibitory activity against HCT116 cells. And the results of multicomponent knockout assay indicated the negative control lost its anti-CRC activity significantly, while the activity can be restored after those 6 compounds were added to the negative control. Conclusions: Those six bufadienolides derivatives are responsible for the anti-CRC HCT116 cells activity of HCS, and are the main components of HCS on anti-CRC. Through this research, we can more clearly understand the main anti-CRC active components in HCS, and the work also lays the foundation for the development of more effective and less toxic medicine from HCS.
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- 2024
- Full Text
- View/download PDF
3. A time-resolved fluorescence microsphere-lateral flow immunochromatographic strip for quantitative detection of Pregnanediol-3-glucuronide in urine samples
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Jiasheng Lin, Sanhua Li, Benchen Ye, Weigang Zheng, Huihui Wang, Ying Liu, Dong Wang, Zaihui Wu, Wen-Fei Dong, and Minghui Zan
- Subjects
time-resolved fluorescence microspheres ,Pregnanediol-3-glucuronide ,lateral flow immunochromatographic ,urine sample ,competitive model ,Biotechnology ,TP248.13-248.65 - Abstract
Introduction: Pregnanediol-3-glucuronide (PdG), as the main metabolite of progesterone in urine, plays a significant role in the prediction of ovulation, threatened abortion, and menstrual cycle maintenance.Methods: To achieve a rapid and sensitive assay, we have designed a competitive model-based time-resolved fluorescence microsphere-lateral flow immunochromatography (TRFM-LFIA) strip.Results: The optimized TRFM-LFIA strip exhibited a wonderful response to PdG over the range of 30–2,000 ng/mL, the corresponding limit of detection (LOD) was calculated as low as 8.39 ng/mL. More importantly, the TRFM-LFIA strip was innovatively used for the quantitative detection of PdG in urine sample, and excellent recovery results were also obtained, ranging from 97.39% to 112.64%.Discussion: The TRFMLFIA strip possessed robust sensitivity and selectivity in the determination of PdG, indicating the great potential of being powerful tools in the biomedical and diagnosis region.
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- 2023
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4. Dioscin integrates regulation of monosaturated fatty acid metabolism to extend the life span through XBP-1/SBP-1 dependent manner
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Yi Xiao, Fang Liu, Xinting Zhu, Sanhua Li, Lingjie Meng, Nian Jiang, Changyan Yu, Haijuan Wang, Ying Qin, Jing Hui, Chunbo Yu, and Yun Liu
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Biological sciences ,Biochemistry ,Physiology ,Science - Abstract
Summary: Delay aging, especially in healthy life extension, brought the most interest to the medical field. Searching for anti-aging drugs with relative safety profiles bring natural products in hotspot. In this study, we find that dioscin promotes the health span extension in wild-type Caenorhabditis elegans. Through the genetic screening in C. elegans, we further reveal that dioscin activates the transcription factor SBP-1/SREBP by the UPRER transcription factor XBP-1 to upregulate transcription of the Δ9 desaturase FAT-5 and FAT-7, resulting in increased monounsaturated fatty acid content which requires for healthy life span extension. Intriguingly, through tissue-specific knockdown, we find that dioscin modulates the health span by activating SBP-1 in the intestine. Unexpectedly, dietary supplementation of POA and OA rescues XBP-1, SBP-1 mutants-induced shortened life span phenotype. Considering the conservation of MUFAs metabolism, dioscin may promote health span in other species, including mammals. Our work suggests that dioscin might be a promising candidate for developing anti-aging agent.
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- 2023
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5. Usnic Acid extends healthspan and improves the neurodegeneration diseases via mTOR/PHA-4 signaling pathway in Caenorhabditis elegans
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Yi Xiao, Huiling Zhang, Yi Sheng, Fang Liu, Jiajun Gao, Guosheng Liu, Sanhua Li, Nian Jiang, Changyan Yu, and Yun Liu
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Biochemistry ,biological sciences ,molecular biology ,neuroscience ,Science - Abstract
Summary: The Mammalian/mechanistic target of rapamycin (mTOR) played a central role in cellular survival and aging. Inhibition of mTOR had been proposed as a reasonable strategy to promote lifespan and delay age-related diseases in evolutionarily diverse organisms. The study showed that lifespan extension and age-related diseases improvement could be achieved by targeting evolutionarily conserved mTOR pathways and mechanisms using pharmacological interventions. Using this approach in Caenorhabditis elegans, We found that 2 μM Usnic Acid significantly extended the healthy lifespan in wild-type animals. Furthermore, via genetic screen, we showed that Usnic Acid acted on mTOR, which was followed by the activation of PHA-4/Foxa to extend the healthy lifespan. Intriguingly, Usnic Acid also delayed neurodegeneration diseases such as Alzheimer’s and polyglutamine disease through mTOR-dependent manner. Our work suggested that Usnic Acid might be a viable candidate for the prevention and treatment of aging and age-related diseases.
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- 2022
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6. Metformin promotes innate immunity through a conserved PMK-1/p38 MAPK pathway
- Author
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Yi Xiao, Fang Liu, Sanhua Li, Nian Jiang, Changyan Yu, Xinting Zhu, Ying Qin, Jing Hui, Lingjie Meng, Changwei Song, Xiao-Fei Li, and Yun Liu
- Subjects
metformin ,innate immunity ,p38 mapk pathway ,c. elegans ,Infectious and parasitic diseases ,RC109-216 - Abstract
Metformin, as the first-line oral drug for type 2 diabetes, has proven benefits against aging, cancer and cardiovascular diseases. But the influence of metformin to the immune response and its molecular mechanisms remain obscure. Metformin increases resistance to not only the Gram-negative pathogens Pseudomonas aeruginosa and Salmonella enterica but also the Gram-positive pathogens Enterococcus faecalis and Staphylococcus aureus. Meanwhile, metformin protects the animals from the infection by enhancing the tolerance to the pathogen infection rather than by reducing the bacterial burden. Through the screening of classical immune pathways in C. elegans, we find metformin enhances innate immunity through p38 MAPK pathway. Furthermore, activated p38/PMK-1 by metformin acts on the intestine for innate immune response. In addition, metformin-treated mice have increased resistance to P. aeruginosa PA14 infection and significantly increased the levels of active PMK-1. Therefore, promoted p38/PMK-1-mediated innate immunity by metformin is conserved from worms to mammals. Our work provides a conserved mechanism by which metformin enhances immune response and boosts its therapeutic application in the treatment of pathogen infection.
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- 2020
- Full Text
- View/download PDF
7. Sanguinarine promotes healthspan and innate immunity through a conserved mechanism of ROS-mediated PMK-1/SKN-1 activation
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Fang Liu, Haijuan Wang, Xinting Zhu, Nian Jiang, Feng Pan, Changwei Song, Chunbo Yu, Changyan Yu, Ying Qin, Jing Hui, Sanhua Li, Yi Xiao, and Yun Liu
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Biological sciences ,Molecular biology ,Immunology ,Science - Abstract
Summary: The longevity of an organism is influenced by both genetic and environmental factors. With respect to genetic factors, a significant effort is being made to identify pharmacological agents that extend lifespan by targeting pathways with a defined role in the aging process. Sanguinarine (San) is a benzophenanthridine alkaloid that exerts a broad spectrum of properties. In this study, we utilized Caenorhabditis elegans to examine the mechanisms by which sanguinarine influences aging and innate immunity. We find that 0.2 μM sanguinarine extends healthspan in C. elegans. We further show that sanguinarine generates reactive oxygen species (ROS), which is followed by the activation of PMK-1/SKN-1pathway to extend healthspan. Intriguingly, sanguinarine increases resistance to pathogens by reducing the bacterial burden in the intestine. In addition, we also find that sanguinarine enhances innate immunity through PMK-1/SKN-1 pathway. Our data suggest that sanguinarine may be a viable candidate for the treatment of age-related disorders.
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- 2022
- Full Text
- View/download PDF
8. The combination of Biochanin A and SB590885 potentiates the inhibition of tumour progression in hepatocellular carcinoma
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Yi Xiao, Qiang Gong, Wenhong Wang, Fang Liu, Qinghong Kong, Feng Pan, Xiaoke Zhang, Changyan Yu, Shanshan Hu, Fang Fan, Sanhua Li, and Yun Liu
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Biochanin A ,SB590885 ,Hepatocellular carcinoma ,Combination therapeutic ,ERK MAPK pathway ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Cytology ,QH573-671 - Abstract
Abstract Background Hepatocellular carcinoma (HCC) is the most aggressive and frequently diagnosed malignancy of the liver. Despite aggressive therapy, life expectancy of many patients in these cases is extended by only a few months. Hepatocellular carcinoma (HCC) has a particularly poor prognosis and would greatly benefit from more effective therapies. Methods The CCK-8 assay and colony formation assays were used to test the cell proliferation and viability. The effects of combination Biochanin A and SB590885 on apoptosis and cell cycle arrest of HCC cells were analysed by flow cytometry. The expression of ERK MAPK and PI3K/AKT/mTOR signalling as well as apoptosis and cell cycle-related proteins in HCC cells were tested by western blotting. The HCC cell xenograft model was established to test the tumor proliferation. Serum and plasma were tested for liver and kidney safety markers (ALP, ALT, AST, total bilirubin, creatinine, urea nitrogen) by using SpectraMax i3X. Results The combination of natural product Biochanin A with the BRAF inhibitor SB590885 synergistically suppressed proliferation, and promoted cell cycle arrest and apoptosis in vitro. Furthermore, we demonstrated that the combination of Biochanin A and SB590885 led to increased impairment of proliferation and HCC tumour inhibition through disrupting of the ERK MAPK and the PI3K/AKT pathways in vitro. The volumes tumors and the weights of tumours were significantly reduced by the combination treatment compared to the control or single treatments in vivo. In addition, we found that there was no significant hepatorenal toxicity with the drug combination, as indicated by the hepatorenal toxicity test. Conclusion The results identify an effective combination therapy for the most aggressive form of HCC and provide the possibility of therapeutic improvement for patients with advanced HCC.
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- 2020
- Full Text
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9. Bufogarlides A-C, three new bufadienolides with Δ14,15 double bond from the skins of Bufo bufo gargarizans
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Lingjie Meng, Qinghong Kong, Jianxun Xie, Feng Pan, Keyu Lu, Sanhua Li, Yiling Li, Di Zhang, and Yun Liu
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Organic Chemistry ,Plant Science ,Biochemistry ,Analytical Chemistry - Abstract
Three new bufadienolides with a Δ14,15 double bond, named bufogarlides A-C (1-3), together with three known analogs (4-6), were isolated from the skins of Bufo bufo gargarizans. Their structures were identified by analyses of spectroscopic data (1 D and 2 D NMR, HR-ESIMS), and comparison with the literature data. All the isolates were evaluated for their cytotoxic activities against ovarian carcinoma cell lines A2780 and SKOV3. Among them, compound 5 showed the highest potential for the growth inhibition of cancerous cells A2780 and SKOV3 with the IC50 values of 21.09 and 67.08 nM, respectively.
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- 2022
10. Microstructure and property of Cu–2.7Ti–0.15Mg–0.1Ce–0.1Zr alloy treated with a combined aging process
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Si, Li, Zhou, Li, Zhu, Xiao, Sanhua, Li, Leinuo, Shen, and Qiyi, Dong
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- 2016
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11. 3-epi-bufotalin suppresses the proliferation in colorectal cancer cells through the inhibition of the JAK1/STAT3 signaling pathway
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SANHUA LI, QINGHONG KONG, XIAOKE ZHANG, XINTING ZHU, CHUNBO YU, CHANGYAN YU, NIAN JIANG, JING HUI, LINGJIE MENG, and YUN LIU
- Subjects
General Medicine - Published
- 2022
12. Brevilin A enhances innate immunity and the resistance of oxidative stress in Caenorhabditis elegans via p38 MAPK pathway
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Xinting Zhu, Fang Liu, Qinyi Wu, Sanhua Li, Guoyong Ruan, Jianbo Yang, Changyan Yu, Nian Jiang, Yi Xiao, and Yun Liu
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Pharmacology ,Mammals ,Oxidative Stress ,Immunology ,Immunology and Allergy ,Animals ,Caenorhabditis elegans ,Caenorhabditis elegans Proteins ,p38 Mitogen-Activated Protein Kinases ,Immunity, Innate - Abstract
The conserved p38/PMK-1 pathway that is an evolutionarily conserved module used by mammals and nematodes in immune response against bacterial infections. Brevilin A (BA), a sesquiterpene lactone compound of Centipeda minima has been shown to exhibit activities such as anti-tumor, anti-bacterial and anti-protozoal. However, whether the Brevilin A influences the immune response and the underlying molecular mechanisms remain obscure. We find that 10 μM Brevilin A increases resistance to not only the Gram-negative pathogens Pseudomonas aeruginosa and Salmonella enterica but also the Gram-positive pathogens Enterococcusfaecalis and Staphylococcus aureus. Meanwhile, Brevilin A enhances the resistance to pathogens by reducing the bacterial burden in the intestine. Through the genetic screening in C. elegans, we find that Brevilin A promotes innate immunity via p38 MAPK pathway. Furthermore, Brevilin A activates the p38/PMK-1 in the intestine for innate immune response. In addition, we also find that Brevilin A increases the resistance of oxidative stress and extends lifespan through p38 MAPK pathway. Our work suggests that Brevilin A may be a viable candidate for the treatment of infectious diseases.
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- 2022
13. Luteolin promotes pathogen resistance in Caenorhabditis elegans via DAF-2/DAF-16 insulin-like signaling pathway
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Yi Xiao, Li Zhang, Xinting Zhu, Ying Qin, Changyan Yu, Nian Jiang, Sanhua Li, Fang Liu, and Yun Liu
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Pharmacology ,Immunology ,Immunology and Allergy - Published
- 2023
14. Hellebrigenin induces apoptosis in colorectal cancer Cells through induction of excessive reactive oxygen species
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CHUNJIAO LIU, QINHONG KONG, FENG PAN, SHAN JIANG, LINGJIE MENG, GAI HUANG, LIDAN LU, SANHUA LI, and YUN LIU
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General Medicine - Published
- 2021
15. Metformin promotes innate immunity through a conserved PMK-1/p38 MAPK pathway
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Changwei Song, Yi Xiao, Ying Qin, Li Xiaofei, Changyan Yu, Nian Jiang, Jing Hui, Xinting Zhu, Yun Liu, Sanhua Li, Fang Liu, and Lingjie Meng
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endocrine system diseases ,Type 2 diabetes ,Infectious and parasitic diseases ,RC109-216 ,medicine.disease_cause ,p38 Mitogen-Activated Protein Kinases ,Mice ,Enterococcus faecalis ,Pathogen ,innate immunity ,Disease Resistance ,0303 health sciences ,c. elegans ,digestive, oral, and skin physiology ,Salmonella enterica ,3. Good health ,Metformin ,Intestines ,Infectious Diseases ,Pseudomonas aeruginosa ,Mitogen-Activated Protein Kinases ,medicine.drug ,Signal Transduction ,Research Paper ,Microbiology (medical) ,Staphylococcus aureus ,p38 mitogen-activated protein kinases ,Immunology ,Biology ,Microbiology ,p38 mapk pathway ,03 medical and health sciences ,Immune system ,medicine ,Animals ,Caenorhabditis elegans ,Caenorhabditis elegans Proteins ,030304 developmental biology ,Innate immune system ,030306 microbiology ,nutritional and metabolic diseases ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,medicine.disease ,Immunity, Innate ,Mice, Inbred C57BL ,Disease Models, Animal ,Parasitology ,metformin - Abstract
Metformin, as the first-line oral drug for type 2 diabetes, has proven benefits against aging, cancer and cardiovascular diseases. But the influence of metformin to the immune response and its molecular mechanisms remain obscure. Metformin increases resistance to not only the Gram-negative pathogens Pseudomonas aeruginosa and Salmonella enterica but also the Gram-positive pathogens Enterococcus faecalis and Staphylococcus aureus. Meanwhile, metformin protects the animals from the infection by enhancing the tolerance to the pathogen infection rather than by reducing the bacterial burden. Through the screening of classical immune pathways in C. elegans, we find metformin enhances innate immunity through p38 MAPK pathway. Furthermore, activated p38/PMK-1 by metformin acts on the intestine for innate immune response. In addition, metformin-treated mice have increased resistance to P. aeruginosa PA14 infection and significantly increased the levels of active PMK-1. Therefore, promoted p38/PMK-1-mediated innate immunity by metformin is conserved from worms to mammals. Our work provides a conserved mechanism by which metformin enhances immune response and boosts its therapeutic application in the treatment of pathogen infection.
- Published
- 2020
16. Metformin induces S‐adenosylmethionine restriction to extend the Caenorhabditis elegans healthspan through H3K4me3 modifiers
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Yi Xiao, Fang Liu, Qinghong Kong, Xinting Zhu, Haijuan Wang, Sanhua Li, Nian Jiang, Changyan Yu, and Liu Yun
- Subjects
Histones ,S-Adenosylmethionine ,Aging ,Diabetes Mellitus, Type 2 ,Longevity ,Animals ,Cell Biology ,Caenorhabditis elegans ,Caenorhabditis elegans Proteins ,Metformin - Abstract
Metformin, a widely prescribed first-line drug for the treatment of type II diabetes mellitus, has been shown to extend lifespan and delay the onset of age-related diseases. The precisely mechanisms by which these effects are realized remain elusive. We find that metformin exposure is restricted to adults, which is sufficient to extend lifespan. However, limiting metformin exposure to the larvae has no significant effect on Caenorhabditis elegans longevity. Here, we show that after metformin treatment, the level of S-adenosylmethionine (SAM) is reduced in adults but not in the larvae. Potential mechanisms by which reduced SAM might increase lifespan include altering the histone methylation. However, the molecular connections between metformin, SAM limitation, methyltransferases, and healthspan-associated phenotypes are unclear. Through genetic screening of C. elegans, we find that metformin promotes the healthspan through an H3K4 methyltransferase/demethylase complex to downregulate the targets, including mTOR and S6 kinase. Thus, our studies provide molecular links between meformin, SAM limitation, histone methylation, and healthspan and elucidate the mode action of metformin-regulated healthspan extension will boost its therapeutic application in the treatment of human aging and age-related diseases.
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- 2022
17. The combination of Biochanin A and SB590885 potentiates the inhibition of tumour progression in hepatocellular carcinoma
- Author
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Qiang Gong, Xiaoke Zhang, Pan Feng, Fang Liu, Fang Fan, Yun Liu, Qinghong Kong, Wenhong Wang, Shanshan Hu, Sanhua Li, Yi Xiao, and Changyan Yu
- Subjects
Cancer Research ,Combination therapy ,Hepatocellular carcinoma ,Cell ,ERK MAPK pathway ,lcsh:RC254-282 ,Biochanin A ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Genetics ,medicine ,lcsh:QH573-671 ,Protein kinase B ,PI3K/AKT/mTOR pathway ,030304 developmental biology ,0303 health sciences ,Cell growth ,business.industry ,lcsh:Cytology ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,digestive system diseases ,medicine.anatomical_structure ,Oncology ,chemistry ,Apoptosis ,Combination therapeutic ,030220 oncology & carcinogenesis ,Cancer research ,SB590885 ,Primary Research ,business - Abstract
Background Hepatocellular carcinoma (HCC) is the most aggressive and frequently diagnosed malignancy of the liver. Despite aggressive therapy, life expectancy of many patients in these cases is extended by only a few months. Hepatocellular carcinoma (HCC) has a particularly poor prognosis and would greatly benefit from more effective therapies. Methods The CCK-8 assay and colony formation assays were used to test the cell proliferation and viability. The effects of combination Biochanin A and SB590885 on apoptosis and cell cycle arrest of HCC cells were analysed by flow cytometry. The expression of ERK MAPK and PI3K/AKT/mTOR signalling as well as apoptosis and cell cycle-related proteins in HCC cells were tested by western blotting. The HCC cell xenograft model was established to test the tumor proliferation. Serum and plasma were tested for liver and kidney safety markers (ALP, ALT, AST, total bilirubin, creatinine, urea nitrogen) by using SpectraMax i3X. Results The combination of natural product Biochanin A with the BRAF inhibitor SB590885 synergistically suppressed proliferation, and promoted cell cycle arrest and apoptosis in vitro. Furthermore, we demonstrated that the combination of Biochanin A and SB590885 led to increased impairment of proliferation and HCC tumour inhibition through disrupting of the ERK MAPK and the PI3K/AKT pathways in vitro. The volumes tumors and the weights of tumours were significantly reduced by the combination treatment compared to the control or single treatments in vivo. In addition, we found that there was no significant hepatorenal toxicity with the drug combination, as indicated by the hepatorenal toxicity test. Conclusion The results identify an effective combination therapy for the most aggressive form of HCC and provide the possibility of therapeutic improvement for patients with advanced HCC.
- Published
- 2020
18. On a binary Diophantine inequality involving prime numbers
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Sanhua Li and Yingchun Cai
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Algebra and Number Theory ,Lebesgue measure ,010102 general mathematics ,Prime number ,Binary number ,0102 computer and information sciences ,01 natural sciences ,Combinatorics ,Number theory ,010201 computation theory & mathematics ,Diophantine inequality ,Physics::Atomic Physics ,0101 mathematics ,Mathematics - Abstract
Let $$1< c < \frac{59}{44},\, c\ne \frac{4}{3}$$ . In this paper it is proved that for any sufficiently large real N, for almost all real $$T\in (N,2N]$$ (in the sense of Lebesgue measure), the inequality $$|p_1^{c} + p_2^{c} - T|< T^{-\frac{9}{10c}(\frac{59}{44}-c)}$$ is solvable in primes $$p_1, p_2$$ . This result constitutes an improvement upon that of Kumchev and Laporta.
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- 2020
19. Two new 19-hydroxy bufadienolides with cytotoxic activity from the skins of Bufo melanosticus
- Author
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Qinghong Kong, Wei Yu, Mei Wang, Nian Jiang, Xinting Zhu, Yun Liu, Lingjie Meng, Sanhua Li, Nian Li, Xiaoke Zhang, and Zeli Chun
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biology ,010405 organic chemistry ,Stereochemistry ,Chemistry ,Organic Chemistry ,Plant Science ,biology.organism_classification ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Analytical Chemistry ,010404 medicinal & biomolecular chemistry ,Cytotoxic T cell ,Bufo - Abstract
Two new 19-hydroxy bufadienolides, named bufohydrolide A (1) and bufohydrolide B (2), and four known analogs (3-6) were isolated from the aqueous extracts of the skins of Bufo melanosticus. Their structures were established by spectral data analyses, such as UV, IR, 1 D/2D NMR and mass spectra. Compounds 1-6 were evaluated for their cytotoxic activity against SMMC-7721, HT-29 and A549 cells. Noteworthily, all six isolates exhibited various levels of anti-proliferative activities with IC50 values ranging from 0.02 ± 0.0002 to 25 ± 0.5 μM.
- Published
- 2020
20. Purification and the effects on structure and bioactivity for polysaccharide from Actinidia valvata Dunn. using macroporous adsorption resin
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Feng PAN, Sanhua LI, Xinting ZHU, Jianbo YANG, Jing WEN, Changwei SONG, Xirong LUO, Guoyong RUAN, and Yun LIU
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decoloration ,polysaccharide from Actinidia valvata dunn ,deproteinization ,macroporous resin ,antioxidant and anticancer activities ,Food Science ,Biotechnology - Abstract
Resin AB-8 was screened from seven resins with different polarity and particle size because of its better decoloration and deproteinization efficiency to polysaccharide from the root of Actinidia valvata (AvPs), and then the optimized parameter conditions were applied by the single-factor experiments and were shown as follows: a concentration of crude polysaccharide of 3 mg/mL, an adsorption time of 40 min, an initial pH of 7.0, a flow rate of 1.5 BV/h. In addition, the results of HPGPC, FT-IR, PMP-HPLC-DAD, 1H NMR and Congo red test showed that the sugar chain, structural group, monosaccharide composition and triple-helix conformation structure of AvPs were not broken during resin AB-8 treatment. Moreover, AvPs could keep the stability of biological activity after the resin AB-8 treatment, and revealed an obviously antioxidant and anticancer activities in vitro as well. This research suggests that this method is promising for the deproteinization and decolorization of AvPs which could be explored as a potential antioxidant or anticancer agent for use in functional food or medicine.
- Published
- 2022
21. Sanguinarine promotes healthspan and innate immunity through a conserved mechanism of ROS-mediated PMK-1/SKN-1 activation
- Author
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Fang Liu, Haijuan Wang, Xinting Zhu, Nian Jiang, Feng Pan, Changwei Song, Chunbo Yu, Changyan Yu, Ying Qin, Jing Hui, Sanhua Li, Yi Xiao, and Yun Liu
- Subjects
Biological sciences ,Multidisciplinary ,Molecular biology ,Science ,Immunology - Abstract
Summary: The longevity of an organism is influenced by both genetic and environmental factors. With respect to genetic factors, a significant effort is being made to identify pharmacological agents that extend lifespan by targeting pathways with a defined role in the aging process. Sanguinarine (San) is a benzophenanthridine alkaloid that exerts a broad spectrum of properties. In this study, we utilized Caenorhabditis elegans to examine the mechanisms by which sanguinarine influences aging and innate immunity. We find that 0.2 μM sanguinarine extends healthspan in C. elegans. We further show that sanguinarine generates reactive oxygen species (ROS), which is followed by the activation of PMK-1/SKN-1pathway to extend healthspan. Intriguingly, sanguinarine increases resistance to pathogens by reducing the bacterial burden in the intestine. In addition, we also find that sanguinarine enhances innate immunity through PMK-1/SKN-1 pathway. Our data suggest that sanguinarine may be a viable candidate for the treatment of age-related disorders.
- Published
- 2021
22. Chemical constituents from Maytenus hookeri
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Nian Jiang, Changyan Yu, Jing Hui, Jianxun Xie, Yuanyuan Wang, Jun Yang, Sanhua Li, and Lingjie Meng
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Biochemistry ,Ecology, Evolution, Behavior and Systematics - Published
- 2022
23. Transdermal BQ-788/EA@ZnO quantum dots as targeting and smart tyrosinase inhibitors in melanocytes
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Nian Jiang, Caixia Yi, Cangyan Yu, Xiao Huang, Sanhua Li, Xingquan Gong, Xi Zheng, Chun Chen, Zuli Xiao, and Xinting Zhu
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Adult ,Materials science ,Biocompatibility ,Tyrosinase ,Biocompatible Materials ,Bioengineering ,02 engineering and technology ,Administration, Cutaneous ,010402 general chemistry ,Endocytosis ,01 natural sciences ,Biomaterials ,Melanin ,chemistry.chemical_compound ,Drug Delivery Systems ,Ellagic Acid ,Piperidines ,Quantum Dots ,Humans ,Distribution (pharmacology) ,Enzyme Inhibitors ,Cells, Cultured ,Transdermal ,Melanins ,Monophenol Monooxygenase ,021001 nanoscience & nanotechnology ,Controlled release ,0104 chemical sciences ,chemistry ,Mechanics of Materials ,Delayed-Action Preparations ,Biophysics ,Melanocytes ,Tyrosine ,Zinc Oxide ,0210 nano-technology ,Oligopeptides ,Ellagic acid - Abstract
Tyrosinase inhibitors could effectively limit the activity of tyrosinase in melanocytes to reduce the excessive synthesis and deposition of melanin. However, low skin permeability and lacking in targeting greatly restricted their application. Herein, ZnO quantum dots were synthesized by gel-sol method and grafted with BQ-788, which have been employed as transdermal and targeting carrier to delivery ellagic acid to melanocytes. Ellagic acid loaded ZnO quantum dots with the size distribution of around 9 nm could targetedly bind to melanocytes and enter the melanocytes by endocytosis within 1 h. The ellagic acid release behavior was controlled by the decreasing of pH via the rapid dissolution of ZnO. When the concentration of BQ-788/EA@ZnO was 12.5 μg/mL, the inhibition rate on tyrosinase activity and melanin deposition were up to 44.23 ± 4.97% and 37.50 ± 5.23%, respectively. In view of their good biocompatibility, they were of great potential in clinically external application for tyrosinase inhibition.
- Published
- 2019
24. On a Diophantine equation with prime numbers
- Author
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Sanhua Li
- Subjects
Algebra and Number Theory ,Computer Science::Information Retrieval ,Diophantine equation ,010102 general mathematics ,Astrophysics::Instrumentation and Methods for Astrophysics ,Prime number ,Computer Science::Computation and Language (Computational Linguistics and Natural Language and Speech Processing) ,01 natural sciences ,Prime (order theory) ,010101 applied mathematics ,Combinatorics ,TheoryofComputation_MATHEMATICALLOGICANDFORMALLANGUAGES ,Exponential sum ,ComputingMethodologies_SYMBOLICANDALGEBRAICMANIPULATION ,ComputingMethodologies_DOCUMENTANDTEXTPROCESSING ,Computer Science::General Literature ,0101 mathematics ,ComputingMilieux_MISCELLANEOUS ,Mathematics ,Real number - Abstract
Let [Formula: see text] denote the integral part of the real number [Formula: see text]. In this paper, it is proved that for [Formula: see text], the Diophantine equation [Formula: see text] is solvable in prime variables [Formula: see text] for sufficiently large integer [Formula: see text].
- Published
- 2019
25. Bufadienolides from the skins of Bufo melanosticus and their cytotoxic activity
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Jing Hui, Liu Yun, Changwei Song, Yi Xiao, Zeli Chun, Xinting Zhu, Changyan Yu, Ying Qin, Sanhua Li, Nian Jiang, and Lingjie Meng
- Subjects
Chromatography ,Toad skin ,biology ,Chemistry ,Plant Science ,biology.organism_classification ,Biochemistry ,Nmr data ,Carcinoma Cell ,Ic50 values ,Cytotoxic T cell ,Bufo ,Agronomy and Crop Science ,Biotechnology - Abstract
Three new bufadienolides, (2β,5β,14β)-2,5,14-trihydroxy-bufa-20,22-dienolide (1), (3α,16β)-3,16-dihydroxy-bufa-14,20,22-trienolide (2) and (3β,5β,16α)-3,5,16-trihydroxy-bufa-14,20,22-trienolide (3), together with three known analogs (4-6), were isolated from the aqueous extracts of the skins of Bufo melanosticus. Their structures were determined by spectroscopic studies, including HR-ESIMS and NMR data analyse. In the preliminary assay, all the above mentioned compounds showed cytotoxic activity against the carcinoma cell lines Bel-7402, HO-8910 and HCT-116, with IC50 values ranging from 0.01 to 24.07 μM, consistent with the practical application of toad skin and cinobufacini (Hua Chan Su).
- Published
- 2019
26. On a Diophantine inequality involving prime numbers
- Author
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Sanhua Li and Yingchun Cai
- Subjects
Algebra and Number Theory ,010102 general mathematics ,Prime number ,0102 computer and information sciences ,01 natural sciences ,Prime (order theory) ,Combinatorics ,Exponential sum ,Number theory ,010201 computation theory & mathematics ,Diophantine inequality ,0101 mathematics ,Constant (mathematics) ,Mathematics - Abstract
Let 1 0 such that for each real numberN>N(c) the inequality\(|p_1^c + p_2^c + p_3^c - N|< N^{ - \tfrac{1}{c}(\tfrac{{11}}{{10}} - c)} \log ^{c_1 } N\) is solvable in prime numbersp 1,p 2,p 3, wherec 1 is some absolute positive constant.
- Published
- 2019
27. miR-487a promotes progression of gastric cancer by targeting TIA1
- Author
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Mingda Wang, Dongjie Yao, Jin Li, Rui Xie, Yun Liu, Sanhua Li, Xuefeng Yang, Shouyang Yu, Bohao Lin, and Xianchun Tang
- Subjects
Male ,0301 basic medicine ,TIA1 ,Tumor suppressor gene ,Mice, SCID ,Biochemistry ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Stomach Neoplasms ,medicine ,Animals ,Humans ,RNA, Neoplasm ,Psychological repression ,Gene knockdown ,Cell growth ,Chemistry ,Cancer ,General Medicine ,medicine.disease ,Neoplasm Proteins ,T-Cell Intracellular Antigen-1 ,MicroRNAs ,030104 developmental biology ,Apoptosis ,030220 oncology & carcinogenesis ,Cancer research ,Female ,Intracellular - Abstract
Gastric cancer (GC) is one of the most common malignancies as well as the third leading cause for cancer-related death. Molecular basis of GC are essential and critical for its therapeutic treatment, but still remain poorly understood. T-cell intracellular antigen-1 (TIA1) extensively involves in cancer progression, whereas its role and regulation mechanism in GC have not been revealed. In the present study, we found that TIA-1 protein level was down-regulated in GC tissues and TIA1 inhibited proliferation and promoted apoptosis of GC cells. Then, we used bioinformatics to predict miR-487a as the upstream regulator of TIA1 and we also observed an inverse correlation between miR-487a level and TIA-1 protein level in GC tissues. Next, we demonstrated that miR-487a directly targeted TIA1 via binding to its 3′-untranslated region. Furthermore, we investigated the role of miR-487a-TIA1 pathway in the growth of GC cells both in vitro and in vivo. The repression of TIA-1 by miR-487a promoted cell proliferation and suppressed cell apoptosis in vitro, and the knockdown of miR-487a had the opposite effects. Finally, we demonstrated that miR-487a promoted the development of gastric tumor growth in xenograft mice by targeting TIA-1. These effects could be partially reversed by restoring the expression of TIA-1. Overall, our results reveal that TIA1 is a tumor suppressor gene and is directly regulated by miR-487a in GC, which may offer new therapeutic targets for GC treatment.
- Published
- 2018
28. Two new 19-hydroxy bufadienolides with cytotoxic activity from the skins of
- Author
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Lingjie, Meng, Sanhua, Li, Qinghong, Kong, Mei, Wang, Xiaoke, Zhang, Xinting, Zhu, Wei, Yu, Nian, Jiang, Zeli, Chun, Nian, Li, and Yun, Liu
- Subjects
Bufanolides ,Molecular Structure ,Animals ,Antineoplastic Agents ,Bufonidae ,Skin - Abstract
Two new 19-hydroxy bufadienolides, named bufohydrolide A (
- Published
- 2020
29. Additional file 1 of The combination of Biochanin A and SB590885 potentiates the inhibition of tumour progression in hepatocellular carcinoma
- Author
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Xiao, Yi, Gong, Qiang, Wenhong Wang, Liu, Fang, Qinghong Kong, Pan, Feng, Xiaoke Zhang, Changyan Yu, Shanshan Hu, Fan, Fang, Sanhua Li, and Liu, Yun
- Abstract
Additional file 1: Table S1. Synergistic indexes of combination treatment with Biochanin A and SB590885 in Bel-7402 and Sk-Hep1 hepatocellular carcinoma cell lines. Table S2. Clinical and biological analyses. Table S3. The STR test results of SK-Hep-1 cells. Table S4. The STR test results of Bel‑7402 cells.
- Published
- 2020
- Full Text
- View/download PDF
30. Synergistic activity of magnolin combined with B-RAF inhibitor SB590885 in hepatocellular carcinoma cells via targeting PI3K-AKT/mTOR and ERK MAPK pathway
- Author
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Wenhong, Wang, Yi, Xiao, Sanhua, Li, Xinting, Zhu, Lingjie, Meng, Changwei, Song, Changyan, Yu, Nian, Jiang, and Yun, Liu
- Subjects
Original Article ,digestive system diseases - Abstract
The prognosis of patients with advanced hepatocellular carcinoma (HCC) remains obscure. From a clinical point of view, the ERK MAPK pathway and the PI3K/AKT pathway are activated in the majority of liver cancer. In addition, long term used to single agent treatment of HCC, frequently results in reverse activation of the ERK MAPK pathway or the PI3K/AKT pathway, leading to drug resistance. Thus, it is important to research the mechanism of combination agents that could suppress different pathways to treat HCC. Here, we found that combination natural product magnolin with BRAF inhibitor SB590885 synergistically suppressed the proliferation, promoted cell cycle arrest and apoptosis in hepatocellular carcinoma cells Bel-7402 and SK-Hep1. Furthermore, we demonstrated that the magnolin and the SB590885 combination led to increased impaired proliferation via inhibition of the ERK MAPK pathway and the PI3K/AKT pathway. These findings highlight the important role of agent combination and provided the approaches of therapeutic improvement for patients with advanced HCC.
- Published
- 2019
31. Research on EEMD for vibration extreme feature preserving of rotating machinery
- Author
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Sanhua Li, Chunhua Tian, Chen Wang, and Zaichao Ma
- Subjects
Vibration ,Feature (computer vision) ,business.industry ,Computer science ,Pattern recognition ,Artificial intelligence ,business - Abstract
Ensemble Empirical Mode Decomposition (EEMD) has been widely used due to the more advanced processing of mode mixing than Empirical Mode Decomposition (EMD). However, EEMD still suffers mode mixing in the vibration analysis of rotating machinery. It proposes Extremum Feature Preserving Ensemble Empirical Mode Decomposition (EFPEEMD) for the feature extraction of rotor systems in rotating machinery. In the method, a fluctuant variation (FV) index is developed to quantitatively recognize the amplitude of added noise on the basis of the empirical rules in EEMD. The results of a misalignment vibration signal of a rotor system and an inner race defect signal of a rolling bearing show that, the primary vibration modes can be extracted rapidly and independently based on the elimination of the non-stationary and non-linear interference. Moreover, the fluctuant variation index removes the originally manual and empirical steps, thus enhancing the accuracy and automation of EEMD on the basis of preserving the extreme information.
- Published
- 2020
32. Comparison of antioxidant and anticancer of the extracts from Various Habitats of Selaginella doederleinii
- Author
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Die Guo, Sanhua Li, Mengmei Shen, Gang Wang, Yingbiao Tian, and Honglian Zhou
- Subjects
Antioxidant ,ABTS ,Traditional medicine ,DPPH ,medicine.medical_treatment ,Extraction (chemistry) ,Ethyl acetate ,Selaginella doederleinii ,Biology ,law.invention ,chemistry.chemical_compound ,chemistry ,law ,medicine ,Essential oil - Abstract
In this study, aioxidant and anticancer activities of the extracts were evaluated from various habitats of Selaginella doederleinii. The evaluation on antioxidative capacity revealed that the extracts were the highest from Guizhou province using DPPH radical scavenging, ABTS radical scavenging, ferric reducing and FRAP methods, while those of the extracts from Sichuan province were the lowest from different habitats. The evaluation on antitumous effect showed that the extracts from Guizhou province were the highest against A549 cell line and 7721 cell line, while those of the extracts from Sichuan province were the lowest in different habitats. Results obtained revealed that the extracts of S. doederleinii were found to be potentially as a readily valuable bioactive source of natural products. Ethyl acetate extraction of S. doederleinii exhibited as the most potent fraction will be further in-depth study.
- Published
- 2016
33. Genome analysis and gene nblA identification of Microcystis aeruginosa myovirus (MaMV-DC) reveal the evidence for horizontal gene transfer events between cyanomyovirus and host
- Author
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Qi-Ya Zhang, Sanhua Li, Tong Ou, and Xiao-Chan Gao
- Subjects
Regulation of gene expression ,Whole genome sequencing ,Genetics ,Gene Expression Regulation, Viral ,Microcystis ,Gene Transfer, Horizontal ,Cyanophage ,Genome, Viral ,Biology ,Genome ,Viral Proteins ,RNA, Transfer ,Virology ,Myoviridae ,Phycocyanin ,Horizontal gene transfer ,DNA, Viral ,RNA, Viral ,Phycobilisome ,Gene ,Phylogeny - Abstract
The genome sequence, genetic characterization and nblA gene function of Microcystis aeruginosa myovirus isolated from Lake Dianchi in China (MaMV-DC) have been analysed. The genome DNA is 169 223 bp long, with 170 predicted protein-coding genes (001L–170L) and a tRNA gene. About one-sixth of these genes have homologues in the host cyanobacteria M. aeruginosa. The genome carries a gene homologous to host nblA, which encodes a protein involved in the degradation of cyanobacterial phycobilisome. Its expression during MaMV-DC infection was confirmed by reverse transcriptase PCR and Western blot detection and abundant expression was companied by the significant decline of phycocyanin content and massive release of progeny MaMV-DC. In addition, expressing MaMV-DC nblA reduced the phycocyanin peak and the phycocyanin to chlorophyll ratio in model cyanobacteria. These results confirm that horizontal gene transfer events have occurred between cyanobacterial host and cyanomyovirus and suggest that MaMV-DC carrying host-derived genes (such as 005L, that codes for NblA) is responsible for more efficient expression of cyanophage genes and release of progeny cyanophage. This study provides novel insight into the horizontal gene transfer in cyanophage and the interactions between cyanophage and their host.
- Published
- 2015
34. Two virus-like particles that cause lytic infections in freshwater cyanobacteria
- Author
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Qi-Ya Zhang, Tong Ou, and Sanhua Li
- Subjects
Cyanobacteria ,Letter ,biology ,Host (biology) ,Immunology ,Virion ,Fresh Water ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Virus ,Host Specificity ,Microbiology ,Bacteriolysis ,Lytic cycle ,Fresh water ,Microscopy, Electron, Transmission ,Virology ,Ultrastructure ,bacteria ,Molecular Medicine ,Microcystis aeruginosa ,Bacteriophages ,Anabaena spiroides - Abstract
We report the results of the preliminary isolation of two virus-like particles (VLPs) that are infectious to freshwater cyanobacteria from Lake Donghu,the largest urban lake in China,located in Wuhan City,Hubei Province. By light and transmission electron microscopy,we observed VLPs causing lytic infections in freshwater bloom-forming cyanobacteria,and we detected their infections by exposing the VLPs to 12 cyanobacterial strains,including Microcystis aeruginosa HAB1801 and Anabaena spiroides HAB1211. They were termed ‘Anabaena spiroides geminivirus-like (AsGV-L)' particles and ‘Microcystis aeruginosa corticovirus-like (MaCV-L)' particles,based on their ultrastructural morphological characteristics and host specificities.
- Published
- 2013
35. Cultivation and characterization of the MaMV-DC cyanophage that infects bloom-forming cyanobacterium Microcystis aeruginosa
- Author
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Xiangyong Liao, Tong Ou, Sanhua Li, and Qi-Ya Zhang
- Subjects
Cyanobacteria ,China ,Microcystis ,Immunology ,Restriction Mapping ,Viral Plaque Assay ,Host Specificity ,Microbiology ,chemistry.chemical_compound ,Restriction map ,Microscopy, Electron, Transmission ,Virology ,Microcystis aeruginosa ,Sodium dodecyl sulfate ,Polyacrylamide gel electrophoresis ,Electrophoresis, Agar Gel ,Viral Structural Proteins ,biology ,Virion ,Cyanophage ,biology.organism_classification ,Restriction enzyme ,Lakes ,chemistry ,Agarose gel electrophoresis ,DNA, Viral ,Molecular Medicine ,Electrophoresis, Polyacrylamide Gel ,Research Article - Abstract
The MaMV-DC cyanophage, which infects the bloom-forming cyanobacterium Microcystis aeruginosa , was isolated from Lake Dianchi, Kunming, China. Twenty-one cyanobacterial strains were used to detect the host range of MaMV-DC. Microcystic aeruginosa FACHB-524 and plaque purification were used to isolate individual cyanophages, and culturing MaMV-DC with cyanobacteria allowed us to prepare purified cyanophages for further analysis. Electron microscopy demonstrated that the negatively stained viral particles are tadpole-shaped with an icosahedral head approximately 70 nm in diameter and a contractile tail approximately 160 nm in length. Using one-step growth experiments, the latent period and burst size of MaMV-DC were estimated to be 24 -48 hours and approximately 80 infectious units per cell, respectively. Restriction endonuclease digestion and agarose gel electrophoresis were performed using purified MaMV-DC genomic DNA, and the genome size was estimated to be approximately 160 kb. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed four major structural proteins. These results support the growi ng interest in using freshwater cyanophages to control bloom-forming cyanobacterium.
- Published
- 2013
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