Search

Non-alcoholic fatty liver disease (NAFLD), also known as hepatic steatosis, is highly prevalent in developed countries despite advancements in clinical modalities. Therefore, there is a need for identifying the bioactive molecular entity (BME) that can therapeutically intervene with liver steatosis progression. In this study, we investigated the efficacy of one such BME - ellagic acid (EA) to ascertain its molecular therapeutic potential against iodoacetamide (IAA) mediated liver steatosis in an adult zebrafish model. Dysregulation of lipid homeostasis by IAA and its amelioration by EA was examined by histological staining and biochemical analysis in the adult zebrafish model. Furthermore, the gene expression analysis of 3-hydroxy methyl glutaryl (HMG) CoA reductase, fatty acid synthase and sterol receptor binding protein-1c in IAA mediated liver steatosis and its regulation by EA was also studied by reverse transcription-polymerase chain reaction (RT-PCR). Concurrently, the drug likeliness and pharmacokinetic properties of EA in comparison to Simvastatin (SIM) were analysed computationally by absorption, distribution, metabolism, and excretion (ADME) analysis. Also, the atomic level interactions of HMG-CoA reductase binding pocket with EA in comparison to SIM were examined by the molecular docking approach to ascertain their comparative binding energy (ΔG) and binding pose. Molecular docking revealed prominent hotspot residues (Gly 765, Gln 766, Asp 767, Gly 808) key to both EA and SIM interaction. All the above results revealed that the experimental observations wherein good agreement with the computational analysis substantiating the promising therapeutic potential of EA against IAA mediated liver steatosis.

The Tropical Council for Companion Animal Parasites Ltd. (TroCCAP) is a not-for-profit organisation whose mission is to independently inform, guide and make best-practice recommendations for the diagnosis, treatment and control of companion animal parasites in the tropics and sub-tropics, with the aim of protecting animal and human health. In line with this primary mission, TroCCAP recently developed guidelines for the diagnosis, treatment and control of feline and canine parasites in the tropics. The development of these guidelines required unique and complex considerations to be addressed, often inapplicable to developed nations. Much of the tropics encompass middle-to-low income countries in which poor standards of environmental hygiene and large populations of stray dogs and cats coexist. In these regions, a range of parasites pose a high risk to companion animals, which ultimately may place their owners at risk of acquiring parasitic zoonoses. These considerations led to the development of unique recommendations with regard, for example, to deworming and endoparasite testing intervals for the control of both global and 'region-specific' parasites in the tropics. Moreover, the 'off-' or 'extra'-label use of drugs for the treatment and control of parasitic infections is common practice in many tropical countries and many generic products lack manufacturers' information on efficacy, safety, and quality control. Recommendations and advice concerning the use of such drugs and protocols are also addressed in these guidelines. The formation of these guidelines is an important first step towards improving the education of veterinarians specifically regarding best-practice for the diagnosis, treatment and control of canine and feline parasites in the tropics.

In this study, in-vitro maintenance of fourth instar larvae of T.splendens was undertaken under suitable laboratory conditions. The predatory potential of T.splendens larvae was tested using the larvae of Culex species. For a single predator larva, 50 and 100 numbers of fourth instar larvae of Culex species were given as prey separately. The rate of predation was monitored for five consecutive days using the same prey density. A single fourth instar larvae of T.splendens consumed an average of 26 ± 1.84 to 40 ± 2.67 prey larvae per day. A total number of prey larvae consumed for five days duration were 114 ± 4.62 and 163 ± 3.17 at prey density of 50 and 100 respectively. The predatory impact (PI) values were ranged between 24.62 and 28.24 larvae per day for prey density of 50. Whereas, the PI values were 34.57 and 39.25 larvae per day for prey density of 100. Based on the results, it was concluded that the larvae of T.splendens consumed a good numbers of Culex larvae and it can be included as part of an integrated vector control programme.