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8 results on '"Sandra Kotz"'

1. Überachiever or Developerin: Eye movements during the processing of translingual hybrid noun-formations

Author

Nina Dumrukcic and Sandra Kotzor

Subjects
Hybrid words, Noun-formation, Bilingualism, Eye-tracking, Lexical processing, Science (General), Q1-390, Social sciences (General), H1-99
Abstract

This eye tracking experiment tests how the brain recognizes and processes hybrid German-English word-formations and how this process compares to monolingual items. Thirty bilingual German-English adults from the Oxford area (23 females; mean age = 28.0, SD = 9.3) who were familiar with the meaning and underlying structure of the individual components had no comprehension difficulties. After fitting linear mixed effects models (95 % CI), the results showed an effect of word length and previous exposure to hybrid forms on processing times, indicated by longer fixation times and increased regressions, particularly in later stages of lexical processing. This indicates that bilingual readers have no trouble recognizing hybrid words, but may have difficulty with semantic and syntactic integration due to lack of exposure.

Published
2024
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2. Assessing the contribution of the two protein disulfide isomerases PDIA1 and PDIA3 to cisplatin resistance

Author

Malte Hellwig, Ralf A. Hilger, Ulrich Jaehde, Maximilian Kullmann, Ganna V. Kalayda, Sabine Metzger, and Sandra Kotz

Subjects
Cisplatin, Gene knockdown, Protein Disulfide-Isomerase Family, Chemistry, Medizin, Procollagen-Proline Dioxygenase, Protein Disulfide-Isomerases, Antineoplastic Agents, PDIA3, Biochemistry, Molecular biology, Inorganic Chemistry, Drug Resistance, Neoplasm, Cell Line, Tumor, medicine, Humans, Enzyme Inhibitors, Mode of action, Protein disulfide-isomerase, Cytotoxicity, Intracellular, Protein Binding, medicine.drug
Abstract

Intracellular binding of cisplatin to non-DNA partners, such as proteins, has received increasing attention as an additional mode of action and as mechanism of resistance. We investigated two cisplatin-interacting isoforms of protein disulfide isomerase regarding their contribution to acquired cisplatin resistance using sensitive and resistant A2780/A2780cis ovarian cancer cells. Cisplatin cytotoxicity was assessed after knockdown of either protein disulfide isomerase family A member 1 (PDIA1) or protein disulfide isomerase family A member 3 (PDIA3). Whereas PDIA1 knockdown led to increased cytotoxicity in resistant A2780cis cells, PDIA3 knockdown showed no influence on cytotoxicity. Coincubation with propynoic acid carbamoyl methyl amide 31 (PACMA31), a PDIA1 inhibitor, resensitized A2780cis cells to cisplatin treatment. Determination of the combination index revealed that the combination of cisplatin and PACMA31 acts synergistically. Our results warrant further evaluation of PDIA1 as promising target for chemotherapy, and its inhibition by PACMA31 as a new therapeutic approach.

Published
2015

4. Combination of two-dimensional gel electrophoresis and a fluorescent carboxyfluorescein-diacetate-labeled cisplatin analogue allows the identification of intracellular cisplatin-protein adducts

Author

Ganna V. Kalayda, Sandra Kotz, Barbara Crone, Ulrich Jaehde, Maximilian Kullmann, and Sabine Metzger

Subjects
Cisplatin, Chemotherapy, Protein Disulfide-Isomerase Family, Two-dimensional gel electrophoresis, medicine.medical_treatment, Clinical Biochemistry, Drug resistance, Biology, Pharmacology, Biochemistry, Fluorescence, Analytical Chemistry, Adduct, medicine, Intracellular, medicine.drug
Abstract

Cisplatin is one of the most widely used anticancer agents, but a major problem for successful chemotherapy is the development of drug resistance of tumor cells against cisplatin. Resistance to cisplatin is a multifactorial problem. A method to detect and identify intracellular cisplatin-protein adducts was developed using a fluorescent carboxyfluorescein-diacetate-labeled cisplatin analogue (CFDA-cisplatin), 2DE, and ESI-MS/MS. We identified several CFDA-cisplatin-protein adducts including members of the protein disulfide isomerase family (PDI). These are the first results of the detection of intracellular CFDA-cisplatin-protein adducts, which may help to understand the resistance mechanism of cisplatin.

Published
2015

5. GRP78 knockdown does not affect cytotoxicity of cisplatin in ovarian cancer cells

Author

Malte Hellwig, Sabine Metzger, Sandra Kotz, Ulrich Jaehde, Maximilian Kullmann, and Ganna V. Kalayda

Subjects
Ovarian Neoplasms, Pharmacology, Cisplatin, Oncology, medicine.medical_specialty, Gene knockdown, Chemistry, Antineoplastic Agents, Affect (psychology), Drug Resistance, Neoplasm, Cell Line, Tumor, Internal medicine, Heat shock protein, medicine, Ovarian cancer cells, Cancer research, Humans, Female, Pharmacology (medical), Cytotoxicity, Endoplasmic Reticulum Chaperone BiP, Heat-Shock Proteins, medicine.drug
Published
2015

6. Optimized two-dimensional gel electrophoresis in an alkaline pH range improves the identification of intracellular CFDA-cisplatin-protein adducts in ovarian cancer cells

Author

Ganna V. Kalayda, Ulrich Jaehde, Sabine Metzger, Nadine Dyballa-Rukes, Sandra Kotz, and Maximilian Kullmann

Subjects
0301 basic medicine, Clinical Biochemistry, Biochemistry, Analytical Chemistry, Serine, 03 medical and health sciences, Cell Line, Tumor, medicine, Humans, Electrophoresis, Gel, Two-Dimensional, chemistry.chemical_classification, Cisplatin, Gel electrophoresis, Ovarian Neoplasms, Two-dimensional gel electrophoresis, Chemistry, Isoelectric focusing, Proteins, Metabolism, Hydrogen-Ion Concentration, Fluoresceins, 030104 developmental biology, Enzyme, Female, Isoelectric Focusing, Intracellular, medicine.drug, Protein Binding
Abstract

Intracellular binding of cisplatin to proteins has been associated with acquired resistance to chemotherapy. In our previous study we established an analytical method for the identification of intracellular cisplatin-binding proteins. The method used a fluorescent carboxyfluorescein-diacetate-labeled cisplatin analogue (CFDA-cisplatin), two-dimensional gel electrophoresis (2DE) and mass spectrometry, which allows detecting and identifying intracellular CFDA-cisplatin-containing protein adducts in the acidic pH range (pH 4-7). Based on this analytical method we extended the identification of intracellular cisplatin-protein adducts to the alkaline pH range (pH 6-10) giving chance to discover new important binding partners. 2DE analysis of alkaline proteins is challenging due to the difficult separation of basic proteins during the isoelectric focusing (IEF). The establishment of an optimized IEF protocol for basic proteins enabled us to identify several intracellular CFDA-cisplatin-binding proteins including enzymes of the glucose and serine metabolism like alpha enolase and D-3-phosphoglycerate 1-dehydrogenase.

Published
2017

7. Combination of two-dimensional gel electrophoresis and a fluorescent carboxyfluorescein-diacetate-labeled cisplatin analogue allows the identification of intracellular cisplatin-protein adducts

Author

Sandra, Kotz, Maximilian, Kullmann, Barbara, Crone, Ganna V, Kalayda, Ulrich, Jaehde, and Sabine, Metzger

Abstract

Cisplatin is one of the most widely used anticancer agents, but a major problem for successful chemotherapy is the development of drug resistance of tumor cells against cisplatin. Resistance to cisplatin is a multifactorial problem. A method to detect and identify intracellular cisplatin-protein adducts was developed using a fluorescent carboxyfluorescein-diacetate-labeled cisplatin analogue (CFDA-cisplatin), 2DE, and ESI-MS/MS. We identified several CFDA-cisplatin-protein adducts including members of the protein disulfide isomerase family (PDI). These are the first results of the detection of intracellular CFDA-cisplatin-protein adducts, which may help to understand the resistance mechanism of cisplatin.

Published
2015

8. Asymmetric Influence of Vocalic Context on Mandarin Sibilants: Evidence From ERP Studies

Author

Yaxuan Meng, Sandra Kotzor, Chenzi Xu, Hilary S. Z. Wynne, and Aditi Lahiri

Subjects
LDN, MMN, tongue height, vowel, mandarin sibilant, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract

In the present study, we examine the interactive effect of vowels on Mandarin fricative sibilants using a passive oddball paradigm to determine whether the HEIGHT features of vowels can spread on the surface and influence preceding consonants with unspecified features. The stimuli are two pairs of Mandarin words ([sa] ∼ [ʂa] and [su] ∼ [ʂu]) contrasting in vowel HEIGHT ([LOW] vs. [HIGH]). Each word in the same pair was presented both as standard and deviant, resulting in four conditions (/standard/[deviant]: /sa/[ʂa] ∼ /ʂa/[sa] and /su/[ʂu] ∼ /ʂu/[su]). In line with the Featurally Underspecified Lexicon (FUL) model, asymmetric patterns of processing were found in the [su] ∼ [ʂu] word pair where both the MMN (mismatch negativity) and LDN (late discriminative negativity) components were more negative in /su/[ʂu] (mismatch) than in /ʂu/[su] (no mismatch), suggesting the spreading of the feature [HIGH] from the vowel [u] to [ʂ] on the surface. In the [sa] ∼ [ʂa] pair, however, symmetric negativities (for both MMN and LDN) were observed as there is no conflict between the surface feature [LOW] from [a] to [ʂ] and the underlying specified feature [LOW] of [s]. These results confirm that not all features are fully specified in the mental lexicon: features of vowels can spread on the surface and influence surrounding unspecified segments.

Published
2021
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