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The Mediterranean, a global hotspot for rare ant species, hosts a significant representation of the global diversity of the mainly Holarctic ant genus Temnothorax. However, several groups still require significant taxonomic efforts. The taxonomy of the T. luteus complex species was revised in 2014 when morphometrics allowed distinguishing two valid species and two synonyms out of four taxa that had been originally described from France. The two species recognized since then are T. luteus, distributed from Iberia to the Alps, and the largely sympatric but much more xerothermophilic T. racovitzai. In Italy, only a few records of the complex were ever published, and the identity of the Italian population was never thoroughly assessed. We combined morphometrics with phylogenomic data to assess the identity of the T. luteus populations that spanned from Sicily to the Italian Alps and discovered that all Italian samples belong to a new cryptic species, which we describe as T. apenninicus sp. nov. whose glacial refugium was probably in the southern Apennines.

Social parasitic ants exploit the colonies of other ant species, either permanently or temporarily. The permanent parasites are amongst the rarest species of ants, although their hosts may be very common. Due to their rarity and often restricted distribution range, most of them are listed as vulnerable. Filling in the gaps in geographical and host ranges will advance our understanding of the social parasitic lifestyle's origin and evolution.This study reports the first records of the slave-makers Myrmoxenus algerianus (Cagniant, 1968) for Europe (Italy, Sicily) and M. ravouxi (André 1896) for Albania and the inquiline Anergates atratulus (Schenck, 1852) for Malta (Gozo). We also report new localities of Camponotus universitatis Forel, 1890 for Albania and Myrmoxenus gordiagini Ruzsky, 1902, M. kraussei (Emery, 1915) and Anergates atratulus for Bulgaria. Diversity, type of parasite-host relationships, host range, distribution and conservation of social parasitic ant species in Bulgaria are discussed. Although social parasitic ants are still understudied in Bulgaria, they represent 21% of the regional ant fauna.

Abstract The Eurasian woodcock prefers habitats where its main prey, earthworms, can be found in higher densities. Although they are forest‐dwelling birds, they regularly visit pastures and natural grasslands at night, where earthworm abundance is generally higher. However, there is little information on fine‐scale habitat use in relation to variation in habitat characteristics and prey availability, particularly beyond the breeding season. In our study, we investigated if the nocturnal occurrence of woodcocks during migratory stopover periods differed between two neighbouring fields, or management units, with similar vegetation structure, and if within‐field variation in the spatial patterns of woodcock sightings were associated with fine‐scale earthworm densities and soil parameters. Specifically, we used GPS tracking data of two tagged woodcocks and direct observation data to study patterns of occurrence of birds in a mixed forest‐pasture landscape in Hungary during pre‐ and post‐breeding periods. We compared these patterns with fine‐scale soil characteristics and earthworm abundance, acquired by field sampling. We found that the field with higher earthworm abundance was visited by woodcocks more frequently, and this correlation was similarly observed at the intra‐field level. Our results demonstrate that woodcocks select foraging sites with higher earthworm densities at multiple spatial scales, both between fields (coarse scale), and within fields (fine‐scale). Considering that woodcocks tended to return to the same field to forage at night, the strong associations between occupancy and resources provide a basis for developing habitat management strategies at the field level for conservation. As earthworm densities and soil parameters are good indicators of woodcock foraging habitat, measuring those variables, at least at a coarse scale, could aid in predicting important habitats for the species across the landscape.

In Malaysia, escalating human-wildlife conflicts pose significant risks to both human populations and wildlife species, highlighting the need for advanced systems for effective conflict management. This study introduces the Geo Wild System (GWS), a novel Progressive Web Application (PWA) specifically tailored for Malaysian contexts. GWS integrates data from diverse sources, including citizen reports, park rangers, and licensed hunters, and employs Geographic Information System (GIS) tools to enhance data visualization and identify conflict hotspots. During the study period from January to June 2024, GWS recorded and facilitated the deployment of 117 traps across six districts: Shah Alam, Hulu Selangor, Sungai Besar, Hulu Langat, Georgetown, and Seberang Prai. These deployments, based on reported conflict data, resulted in the capture of 88 wildlife individuals, including 85 long-tailed macaques (Macaca fascicularis), two wild boars (Sus scrofa), and one Asian palm civets (Paradoxurus hermaphroditus), demonstrating the system's operational effectiveness. GWS supports a comprehensive reporting, monitoring, and analysis system, utilizing GIS-based Kernel Density Estimation (KDE) to pinpoint critical conflict hotspots, particularly in rapidly urbanizing areas. User feedback was overwhelmingly positive, highlighting the system’s user-friendliness and robust data integration. Despite initial deployment challenges and geodetic constraints, GWS has proven to be a valuable tool for mitigating wildlife conflicts, enabling informed, timely management responses, and offering a promising approach to adaptive wildlife management in Malaysia.

The paper examines the attitudes and opinions of the representatives of Hungarian-American communities in the United States regarding the officialization of English at the federal level. The corpora of the analysis have been built with the help of a) the websites of Hungarian-American organizations in the United States; b) the legislative database of the US Federal Congress; and the digitized versions of the printed newspapers and magazines published in Hungarian in the United States. The method of classifying the attitude patterns relies on Terrence G. Wiley’s language policy classification spectrum (1999) and on Richard Ruíz’s classic “orientations in language planning” framework (1984), expanded by Hult and Hornberger (2016). The results indicate that while Hungarian-Americans have mostly been trying to maintain their first language (in the private domain), they have also embraced English and willingly assimilated into American society, resenting the alleged ethnolinguistic separatism of Hispanic Americans and their demands for special minority-language accommodations.

Adapting agriculture to climate change is essential for sustainable food production. However, the development of suitable adaptation mechanisms requires a clear understanding of the plant-climate interaction. The number of growing degree days (GDD) is a good proxy for understanding plant-climate interrelationship and farm productivity. Here, using a 2-year experiment of barley-sweet corn double cropping (DC) system and 20-year climate and yield data, we found that barley and sweet corn GDD and productivity were strongly related to changes in climate patterns. Furthermore, we found a positive effect of the barley-sweet corn DC system on farm productivity (18.5 and 5.6 tonnes. ha−1 for DC and single cropping respectively) and return on investment (1.8; 1.4 as benefit:cost ratios for DC and single cropping system respectively). Altogether the results of this study suggest that the winter barley-sweet corn double cropping system is a potential strategy to boost farm productivity as well as an adaptation mechanism to be considered for the changing climate in the study region.

This paper presents numeric morphology-based evidence on the broadly overlapping distribution of two thief ant species Solenopsis fugax (Latreille, 1798) and S. juliae (Arakelian, 1991) in the East European Pontic-Caspian region. The paper integrates two autonomous data collections and independent analyses performed by different researchers, using different equipment, considering different character combinations, and evaluating partially different samples. Five type series, the neotype series of Solenopsis fugax (Latreille 1798) and the type series of S. flavidula (Nylander, 1849), S. (Diplorhoptrum) fugax var. furtiva Santschi, 1934, S. (Diplorhoptrum) fugax var. pontica Santschi, 1934, S. (Diplorhoptrum) fugax var. scytica Santschi, 1934 were nested in one cluster and we propose the junior synonymy of the latter four taxa names with S. fugax. The other cluster contained only one type specimen of Solenopsis nitida (Dlussky & Radchenko, 1994) measured from AntWeb images. The naming of this cluster was based on both verbal statements and measurements of gynes given in the original description of Solenopsis juliae (Arakelian, 1991), which represents the oldest available name for this cluster. Hence, S. nitida is proposed as junior synonym of S. juliae. Solenopsis cypridis Santschi, 1934 is raised to species rank based on investigation of worker and gyne type specimens.

Cavitary corpora lutea are commonly observed during the estrous cycle in bovines. Since the quality of the corpus luteum (CL) is extremely important during embryo transfer when embryos are implanted into the recipient, the ultrasonographic examination of the CL is becoming more and more important in terms of the outcome of the procedure. In the present study, a total of 2477 ultrasonographic transrectal diagnoses were performed, and data were collected between the years of 2018 and 2020 in a large-scale Holstein Friesian dairy farm in Hungary. In 91.1% (n = 2257) and in 8.9% (n = 220) of the cases, compact CLs and cavitary CLs, respectively, were diagnosed at pregnancy diagnosis. The presence of a cavitary CL on the ovary at pregnancy diagnosis increased the odds of remaining open after pregnancy by 21 times compared to the presence of a compact CL (OR = 21.0, p < 0.001) in the cows. The presence of cavitary CL was not influenced either by month or season. Ovarian cysts were detected in 196 cases (8.0%) in the examined animals. The presence of a cavitary CL decreased by 9 times when an ovarian cyst was also diagnosed (OR = 9.0, 1.6% vs. 9.5%, p < 0.001). The presence of an ovarian cyst decreased the odds of established pregnancy by 81 times (OR = 81.1, p < 0.001). Based on our results, the presence of a cavitary CL between days 31 and 42 after artificial insemination is associated with a smaller chance of conception in Holstein Friesian cows. The presence of an ovarian cyst decreases the occurrence of cavitary CL and the chance of conception.

Porcine Reproductive and Respiratory Syndrome (PRRS) significantly impacts the pig farming industry globally, leading to economic losses due to reduced productivity. This study focuses on assessing the presence and impact of PRRS within Hungarian wild boar populations amidst efforts to eradicate the virus from domestic pig herds. We used a combination of serological and virological tests on samples collected from wild boars across Hungary to evaluate the prevalence of PRRS virus and its potential transmission risks to domestic pigs. Our findings reveal a low seropositivity rate in wild boars, suggesting a minimal role of wild boars in the transmission of PRRS to domestic pig populations. Moreover, no relationship was found between domestic pig and wild boar densities, emphasizing the limited interaction and consequent risk of disease spread between these populations. We confirm the effectiveness of Hungary’s PRRS eradication measures among domestic herds and highlight the negligible risk posed by wild boars in re-introducing the PRRS virus.

Abstract Abnormalities of the external genitals are an important issue in dog breeding because of the unfavourable qualities and characteristics of breeds, resulting in consistent economic losses. Despite their significance, little scientific attention has been given to these problems. Although there are several reviews on cryptorchidism in dogs, none have described anorchia. Testicular agenesis is a rare reproductive disorder with a congenital origin. Moreover, no author has described the diagnostic procedure for making a definitive diagnosis of anorchia in dogs. It is important to have a well‐structured diagnostic scheme to help practical veterinarians make a confirmatory diagnosis. This review article aims to provide an update on canine anorchia diagnosis based on the poor research studies published in recent years. We have also contributed to the pathogenesis of this disorder using human medicine studies. Finally, the review includes therapeutic hypotheses that can be expanded in future studies.

Abstract Objectives The aim of this study was to compare the analgesic efficacy of topical irrigation versus injection of lidocaine on the ovarian pedicle to provide analgesia in bitches ovariectomy. In the current study were monitored: increased blood pressure, heart rate and respiratory rate to identify an acute intraoperative nociceptive response. These parameters were registered at six times during the surgical procedure: grasping of the ovary (time 1), dissection of the mesosalpinx (time 2), tightening of the first loop ligature (time 3), tightening of the second loop ligature (time 4), transection of the ovarian pedicle (time 5) and release of the ovary (time 6). Material and Methods Forty healthy bitches were randomly assigned in two groups (n = 20) to receive topical irrigation (splash block) of 2% lidocaine (C group) on both ovarian pedicle (2 mg/kg each), or an equal volume of lidocaine was injected at the same sites (R group). Results The results of the present study suggest that splash block may provide intraoperative analgesic effects equivalent to injection in the ovarian pedicle in dogs that have undergone ovariectomy. The lidocaine improved surgical analgesia during canine ovariectomy in both groups, and this action is not affected by the inoculation technique. Clinical significance Pain management in veterinary patients is a crucial component of appropriate patient care. Therefore, the need for achieving safer anaesthesia for surgical intervention is gaining much attention. Ovariectomy is a common surgical procedure in bitches with medium level of pain. This study concluded that considering its relative simplicity, low cost, and safety, both techniques could be used in daily clinical practice.

Citizen science is a valuable tool for monitoring different species, especially in cases concerning truly rare and difficult-to-detect species where time-consuming field studies are limited and long-term research projects are uncertain. To better understand the distribution of the rarely collected Cryptopone ochracea (Mayr, 1855) (Hymenoptera, Formicidae) in Hungary, we obtained the occurrence data with photos uploaded by non-professionals to the page of the largest Hungarian Facebook group dealing with ants and a citizen-science website dealing with biological data collection. In this article, we expand the known distribution of C. ochracea to include 46 new records from Hungary and one from Serbia. With two historical records, this previously undersampled species has now been found 48 times in Hungary. Our results prove that social media platforms and other websites for citizen science projects offer new and useful opportunities for researchers to involve non-professionals in scientific work and, thus, obtain large amounts of valuable data, even for understudied arthropod species.

Abstract Unprecedented technological advances in digitization and the steadily expanding open‐access digital repositories are yielding new opportunities to quickly and efficiently measure morphological traits without transportation and advanced/expensive microscope machinery. A prime example is the AntWeb.org database, which allows researchers from all over the world to study taxonomic, ecological, or evolutionary questions on the same ant specimens with ease. However, the reproducibility and reliability of morphometric data deduced from AntWeb compared to traditional microscope measurements has not yet been tested. Here, we compared 12 morphological traits of 46 Temnothorax ant specimens measured either directly by stereomicroscope on physical specimens or via the widely used open‐access software tpsDig utilizing AntWeb digital images. We employed a complex statistical framework to test several aspects of reproducibility and reliability between the methods. We estimated (i) the agreement between the measurement methods and (ii) the trait value dependence of the agreement, then (iii) compared the coefficients of variation produced by the different methods, and finally, (iv) tested for systematic bias between the methods in a mixed modeling‐based statistical framework. The stereomicroscope measurements were extremely precise. Our comparisons showed that agreement between the two methods was exceptionally high, without trait value dependence. Furthermore, the coefficients of variation did not differ between the methods. However, we found systematic bias in eight traits: apart from one trait where software measurements overestimated the microscopic measurements, the former underestimated the latter. Our results shed light on the fact that relying solely on the level of agreement between methods can be highly misleading. In our case, even though the software measurements predicted microscope measurements very well, replacing traditional microscope measurements with software measurements, and especially mixing data collected by the different methods, might result in erroneous conclusions. We provide guidance on the best way to utilize virtual specimens (2D z‐stacked images) as a source of morphometric data, emphasizing the method's limitations in certain fields and applications.

Inducible gene regulation methods are indispensable in diverse biological applications, yet many of them have severe limitations in their applicability. These include inducer toxicity, a limited variety of organisms the given system can be used in, and side effects of the induction method. In this study, a novel inducible system, the RuX system, was created using a mutant ligand-binding domain of the glucocorticoid receptor (CS1/CD), used together with various genetic elements such as the Gal4 DNA-binding domain or Cre recombinase. The RuX system is shown to be capable of over 1000-fold inducibility, has flexible applications, and is offered for use in cell cultures.

In this paper, we provide numeric morphology-based evidence that the dark-colored Ponera coarctata var. lucida Emery, 1898, formerly considered a synonym of P. coarctata (Latreille, 1802), is conspecific with the lighter-colored Ponera testacea Emery, 1895. Species hypotheses are developed via NC-PART clustering, combined with Partitioning Algorithm based on Recursive Thresholding (PART), and via PCA combined with gap statistics. We obtained our results from an extensive dataset from the 10 continuous morphometric traits measured on 165 workers belonging to 73 nest samples. Linear discriminant analysis (LDA) confirmed the grouping of hypotheses generated by exploratory analyses with 100% classification success when all ten morphometric traits were involved. The Anatolian Turkish black and the predominantly European yellow samples, did not separate based on their morphometric characteristics. These two color variations broadly overlap in their geographic range in Anatolian Turkey. The investigated type series of Ponera coarctata var. lucida Emery, 1898 (collected from Kazakhstan) fell within the P. testacea cluster instead of P. coarctata and is also classified with high certainty as P. testacea by confirmatory LDA. Therefore, we propose the synonymy of Ponera coarctata var. lucida Emery, 1898 with Ponera testacea Emery, 1895. As no other morphological differences than color patterns were detected between the “black” and “pale” P. testacea samples, we hold that these populations constitute geographically occurring color variations of the same species. Finally, our quantitative morphology-based results show that relying on color patterns is not a robust approach in identifying European Ponera samples, particularly in the east, but using multivariate analyses of morphometric traits is advised instead.

Gentile di Puglia (GdP) is an autochthonous sheep breed of Southern Italy included among ovine breeds threatened by genetic erosion and extinction risk, which have been given attention by local and international institutions, thus emphasizing the need for germplasm conservation actions. In the present study, two assisted reproduction approaches, finalized for GdP conservation, were performed: (1) on-farm reproductive efficiency evaluation, expressed as pregnancy rate (PR), twin pregnancy rate (tPR), and body condition score (BCS), for three consecutive breeding cycles and (2) pre-pubertal lambs’ immature cumulus–oocyte complex (COC) retrieval, vitrification, in vitro maturation (IVM), and assessment of meiotic stage and bioenergetic-oxidative status compared with those of other Italian and European commercial breeds. PR and tPR were progressively reduced over time. In all clinical examination times, BCS was significantly lower in nonpregnant ewes compared with pregnant ones. Fresh GdP pre-pubertal lamb COCs achieved meiotic maturation and showed healthy bioenergetic–oxidative status after IVM. Vitrification reduced the oocyte maturation rate in all groups. However, mature oocytes retained their cytoplasmic maturity, expressed as a mitochondria distribution pattern and activity, indicating promising developmental competence. In conclusion, clinical- and biotechnological-assisted reproduction approaches can support conservation strategies of GdP and other local sheep breeds in Southern Italy.

Abstract Parasitism-generated negative effects on ant societies are multifaceted, implying individual and colony-level responses. Though laboratory based evidence shows that the sublethal fungus Rickia wasmannii is responsible for physiological and behavioral responses that may negatively affect individual workers’ resilience and life expectancy in Myrmica ant workers, colony-level stress response to this parasite is largely unknown. Here, we focus on understanding of a long-term, colony-level effect of Rickia infection on Myrmica scabrinodis ant populations by tracking trait size-based changes. We collected worker specimens from infected and uninfected colonies from the same population in order to: (1) compare body size in response to parasitism, (2) assess the extent to which possible changes in size are associated with the severity of infection, and (3) investigate shifts in body size in response to infection over time by testing correlation of workers’ ages and sizes. We found that workers from infected colonies were significantly smaller than their healthy congeners, but neither infection level nor the age of the workers showed significant correlation with the size in infected colonies. Decreasing body sizes in infected colonies can be ascribed to workers’ mediated effect toward developing larvae, which are unable to attain the average body size before they pupate.

Extant research finds that environmental identity is an important motivational factor for proenvironmental behavior. However, studies typically focus on investigating the effects of the strength of this identity. Based on insights from identity research, we theorize that the influence of individuals’ environmental identity on their proenvironmental behavior may depend on other identity dimensions as well. We argue that the frequency of activation of environmental identity in relevant life domains—environmental identity salience—may predict proenvironmental behavior beyond what environmental identity strength can explain. To test our theorizing, we propose a parsimonious measure of environmental identity salience. In four empirical studies, we establish that the new measure has sound psychometric properties in terms of internal consistency and discriminant validity with regard to measures of environmental identity strength. Importantly, our measure of environmental identity salience reliably predicts a range of self-reported and actual proenvironmental behaviors beyond the effects of environmental identity strength. In line with theoretical predictions, our data suggests that environmental identity salience and strength are related but distinct constructs. We conclude that investigating the nature and effects of environmental identity salience leads to a fruitful path to a more comprehensive understanding of proenvironmental behavior. The proposed new measure may serve as a helpful tool in this endeavor.

A morphological study on the cocoon of Bicyrtes variegatus (Oliver, 1789) is presented based on specimens collected in Center-West Brazil. Notes on nesting habitat is provided. Euschistus heros (Fabricius, 1798) (Hemiptera: Pentatomidae) is recorded as prey, and Traumatomutilla ocellaris (Klug, 1821) (Hymenoptera: Mutillidae) as parasitoid.

Abstract Morphometric research is being applied to a growing number and variety of organisms. Discoveries achieved via morphometric approaches are often considered highly transferable, in contrast to the tacit and idiosyncratic interpretation of discrete character states. The reliability of morphometric workflows in insect systematics has never been a subject of focused research, but such studies are sorely needed. In this paper, we assess the reproducibility of morphometric studies of ants where the mode of data collection is a shared routine. We compared datasets generated by eleven independent gaugers, that is, collaborators, who measured 21 continuous morphometric traits on the same pool of individuals according to the same protocol. The gaugers possessed a wide range of morphometric skills, had varying expertise among insect groups, and differed in their facility with measuring equipment. We used intraclass correlation coefficients (ICC) to calculate repeatability and reproducibility values (i.e., intra‐ and intergauger agreements), and we performed a multivariate permutational multivariate analysis of variance (PERMANOVA) using the Morosita index of dissimilarity with 9,999 iterations. The calculated average measure of intraclass correlation coefficients of different gaugers ranged from R = 0.784 to R = 0.9897 and a significant correlation was found between the repeatability and the morphometric skills of gaugers (p = 0.016). There was no significant association with the magnification of the equipment in the case of these rather small ants. The intergauger agreement, that is the reproducibility, varied between R = 0.872 and R = 0.471 (mean R = 0.690), but all gaugers arrived at the same two‐species conclusion. A PERMANOVA test revealed no significant gauger effect on species identity (R2 = 0.69, p = 0.58). Our findings show that morphometric studies are reproducible when observers follow the standard protocol; hence, morphometric findings are widely transferable and will remain a valuable data source for alpha taxonomy.

Waterlogging in agriculture poses severe threats to soil properties, crop yields, and farm profitability. Remote sensing data coupled with drainage systems offer solutions to monitor and manage waterlogging in agricultural systems. However, implementing agricultural projects such as drainage is associated with high uncertainty and risk, with substantial negative impacts on farm profitability if not well planned. Cost–benefit analyses can help allocate resources more effectively; however, data scarcity, high uncertainty, and risks in the agricultural sector make it difficult to use traditional approaches. Here, we combined a wide range of field and remote sensing data, unsupervised machine learning, and Bayesian probabilistic models to: (1) identify potential sites susceptible to waterlogging at the farm scale, and (2) test whether the installation of drainage systems would yield a positive benefit for the farmer. Using the K-means clustering algorithm on water and vegetation indices derived from Sentinel-2 multispectral imagery, we were able to detect potential waterlogging sites in the investigated field (elbow point = 2, silhouette coefficient = 0.46). Using a combination of the Bayesian statistical model and the A/B test, we show that the installation of a drainage system can increase farm profitability by 1.7 times per year compared to the existing farm management. The posterior effect size associated with yield, cropping area, and time (year) was 0.5, 1.5, and 1.9, respectively. Altogether, our results emphasize the importance of data-driven decision-making for agriculture project planning and resource management in the wake of smart agriculture for food security and adaptation to climate change.

Wildlife have an important role in the lives of local people and conservation practitioners in Malaysia because of their rare and elusive status, socioeconomic impacts, and management conflicts. However, few studies have evaluated the local attitudes toward wildlife and their management methods in Malaysia. In this study, we used indices to measure attitudes toward wildlife and their management methods in Malaysia. The iterative item reliability analysis was executed on online questionnaire data from a random sample of 585 local respondents using Cronbach’s alpha. The result yielded two indices of locals’ attitudes; (i) Wildlife Attitude Index (WAI); and (ii) Wildlife Management Method Attitude Index (WMMAI). The WAI had a Cronbach’s alpha coefficient of 0.71 and the WMMAI had a Cronbach’s alpha coefficient of 0.73. The following variables had significant determinants of WAI and WMMAI in Malaysia: (i) gender; (ii) age; (iii) level of education; (iv) residential area; (v) familiarity (experience); and (vi) nature engagement. These attitude indices could be significant in assisting conservation practitioners and decision-makers in understanding locals’ attitudes to prioritize wildlife management practices and showing the relationship between management and local demographics with the assumption that high-scoring individuals are more likely to favor wildlife conservation initiatives and activities.

Notes on nesting behaviour of Ammophila gracilis Lepeletier de Saint Fargeau, 1845 are provided, based on observations carried out in Rio de Janeiro state, southeastern Brazil, in an area covered with a typical vegetation type that grows in sandy soil of marine origin in coastalplains. Description of stereotyped motors patterns related to nesting behaviour are emphasized. Observations on larval development are also provided.