30 results on '"Sané S"'
Search Results
2. An Effective Multilabel Classification Using Feature Selection
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Sane, S. S., Chaudhari, Prajakta, Tidake, V. S., Kacprzyk, Janusz, Series editor, Pal, Nikhil R., Advisory editor, Bello Perez, Rafael, Advisory editor, Corchado, Emilio S., Advisory editor, Hagras, Hani, Advisory editor, Kóczy, László T., Advisory editor, Kreinovich, Vladik, Advisory editor, Lin, Chin-Teng, Advisory editor, Lu, Jie, Advisory editor, Melin, Patricia, Advisory editor, Nedjah, Nadia, Advisory editor, Nguyen, Ngoc Thanh, Advisory editor, Wang, Jun, Advisory editor, Bhalla, Subhash, editor, Bhateja, Vikrant, editor, Chandavale, Anjali A., editor, Hiwale, Anil S., editor, and Satapathy, Suresh Chandra, editor
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- 2018
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3. Evaluation of Multi-label Classifiers in Various Domains Using Decision Tree
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Tidake, V. S., Sane, S. S., Kacprzyk, Janusz, Series editor, Pal, Nikhil R., Advisory editor, Bello Perez, Rafael, Advisory editor, Corchado, Emilio S., Advisory editor, Hagras, Hani, Advisory editor, Kóczy, László T., Advisory editor, Kreinovich, Vladik, Advisory editor, Lin, Chin-Teng, Advisory editor, Lu, Jie, Advisory editor, Melin, Patricia, Advisory editor, Nedjah, Nadia, Advisory editor, Nguyen, Ngoc Thanh, Advisory editor, Wang, Jun, Advisory editor, Bhalla, Subhash, editor, Bhateja, Vikrant, editor, Chandavale, Anjali A., editor, Hiwale, Anil S., editor, and Satapathy, Suresh Chandra, editor
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- 2018
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4. 11 W narrow linewidth laser source at 780nm for laser cooling and manipulation of Rubidium
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Sané, S. S., Bennetts, S., Debs, J. E., Kuhn, C. C. N., McDonald, G. D., Altin, P. A., Close, J. D., and Robins, N. P.
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Physics - Atomic Physics ,Condensed Matter - Quantum Gases ,Physics - Optics - Abstract
We present a narrow linewidth continuous laser source with over 11 Watts of output power at 780nm, based on single-pass frequency doubling of an amplified 1560nm fibre laser with 36% efficiency. This source offers a combination of high power, simplicity, mode quality and stability. Without any active stabilization, the linewidth is measured to be below 10kHz. The fibre seed is tunable over 60GHz, which allows access to the D2 transitions in 87Rb and 85Rb, providing a viable high-power source for laser cooling as well as for large-momentum-transfer beamsplitters in atom interferometry. Sources of this type will pave the way for a new generation of high flux, high duty-cycle degenerate quantum gas experiments., Comment: 5 pages, 3 figures
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- 2012
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5. Optimization in virtualization
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Ajankar, Sonali, Mohta, Ashish, Sane, S. S., and Pise, S. J., editor
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- 2011
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6. Euler class groups and 2-torsion elements
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Bhatwadekar, S.M., Fasel, J., and Sane, S.
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- 2014
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7. Prolongation of electrode lifetime in biofuel cells by periodic enzyme renewal
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Rubenwolf, S., Sané, S., Hussein, L., Kestel, J., von Stetten, F., Urban, G., Krueger, M., Zengerle, R., and Kerzenmacher, S.
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- 2012
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8. THE EXACT COVERING NUMBER g(1, 6, 11)
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LIMAYE, N. B., SANE, S. S., and WIRMANI-PRASAD, A.
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- 1992
9. Two New Species of Squilla (Stomatopoda) from Bombay
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Chhapgar, B. F. and Sane, S. R.
- Published
- 1967
10. 11 W narrow linewidth laser source at 780 nm for laser cooling and manipulation of Rubidium
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Sané, S S, Bennetts, S, Debs, J E, Kuhn, Carlos C N, McDonald, G D, Altin, P A, Close, J D, Robins, N.P, Sané, S S, Bennetts, S, Debs, J E, Kuhn, Carlos C N, McDonald, G D, Altin, P A, Close, J D, and Robins, N.P
- Abstract
We present a narrow linewidth continuous laser source with over 11 W output power at 780 nm, based on single-pass frequency doubling of an amplified 1560 nm fibre laser with 36% efficiency. This source offers a combination of high power, simplicity, mode quality and stability. Without any active stabilization, the linewidth is measured to be below 10 kHz. The fibre seed is tunable over 60 GHz, which allows access to the D₂ transitions in ⁸⁷Rb and ⁸⁵Rb, providing a viable high-power source for laser cooling as well as for large-momentum-transfer beamsplitters in atom interferometry. Sources of this type will pave the way for a new generation of high flux, high duty-cycle degenerate quantum gas experiments.
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- 2012
11. 11 W narrow linewidth laser source at 780nm for laser cooling and manipulation of Rubidium
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Sané, S. S., primary, Bennetts, S., additional, Debs, J. E., additional, Kuhn, C. C. N., additional, McDonald, G. D., additional, Altin, P. A., additional, Close, J. D., additional, and Robins, N. P., additional
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- 2012
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12. PO66 Triple negative breast cancer in young patients in India
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Mane, A., Deshmukh, S., Zade, B., and Sane, S.
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- 2012
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13. Fluconazole resistant non-albicans vaginal yeast in HIV seronegative women with vaginal discharge in Pune, India
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Kairon, R, Godbole, S, Kulkarni, S, Ghate, M, Sane, S, Shrotri, A, Bharucha, K, Mehendale, S, and Risbud, A
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- 2004
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14. Utility of the point of care CD4 analyzer, PIMA, to enumerate CD4 counts in the field settings in India
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Thakar Madhuri, Mahajan Bharati, Shaikh Nawaj, Bagwan Salman, Sane Suvarna, Kabra Sandhya, Rewari Bharat, Shaukat Mohamad, Singh Namita, Trevor Peter, and Paranjape Ramesh
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CD4+ T-cell count ,HIV ,Point-of-care ,PIMA CD4 analyzer ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Abstract Background In resource limited settings non-availability of CD4 count facility at the site could adversely affect the ART roll out programme. Point of care CD4 enumerating equipments can make the CD4 count available at the site of care and improve the patients’ management considerably. This study is aimed at determining the utility of a Point of Care PIMA CD4 analyzer (Alere, Germany) in the field settings in India. Method The blood samples were collected from 1790 participants at 21 ART centers from different parts of the country and tested using PIMA and the reference methods (FACSCalibur, FACSCount and CyFlow SL3). The paired finger prick and venous blood samples from 175 participants were tested by the PIMA CD4 Analyzer and then by FACSCalibur. Result The CD4 counts obtained by PIMA CD4 analyzer showed excellent correlation with the counts obtained by the reference methods; for venous blood the Pearson’s r was 0.921, p 500 cells/mm3, the differences in the median CD4 counts obtained by the reference method and the PIMA analyzer were not significant (P > 0.05) and the relative bias were low (−7 to 5.1%). The Intermachine comparison showed variation within the acceptable limit of%CV of 10%. Conclusion In the field settings, the POC PIMA CD4 analyzer gave CD4 counts comparable to the reference methods for all CD4 ranges. The POC equipment could identify the patients eligible for ART in 91% cases. Adequate training is necessary for finger prick sample collection for optimum results. Decentralization of CD4 testing by making the CD4 counts available at primary health centers, especially in remote areas with minimum or no infrastructure would reduce the missed visits and improve adherence of the patients.
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- 2012
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15. Stigmatizing attitudes and low levels of knowledge but high willingness to participate in HIV management: A community-based survey of pharmacies in Pune, India
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Gupta Amita, Sane Suvarna S, Gurbani Ajay, Bollinger Robert C, Mehendale Sanjay M, and Godbole Sheela V
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Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background The World Health Organization (WHO) recommends that the role of pharmacists in low-income settings be expanded to address the increasing complexity of HIV antiretroviral (ARV) and co-infection drug regimens. However, in many such settings including in India, many pharmacists and pharmacy workers are often neither well trained nor aware of the intricacies of HIV treatment. The aims of our study were; to determine the availability of ARVs, provision of ARVs, knowledge about ARVs, attitudes towards HIV-infected persons and self-perceived need for training among community-based pharmacies in an urban area of India. Methods We performed a survey of randomly selected, community-based pharmacies located in Pune, India, in 2004-2005 to determine the availability of ARVs at these pharmacies, how they were providing ARVs and their self-perceived need for training. We also assessed knowledge, attitudes and perceptions on HIV and ARVs and factors associated with stocking ARVs. Results Of 207 pharmacies included in the survey, 200 (96.6%) were single, private establishments. Seventy-three (35.3%) pharmacies stocked ARVs and 38 (18.4%) ordered ARVs upon request. The reported median number of ARV pills that patients bought at one time was 30, a two week supply of ARVs (range: 3-240 pills). Six (2.9%) pharmacy respondents reported selling non-allopathic medicines (i.e. Ayurvedic, homeopathy) for HIV. Ninety (44.2%) pharmacy respondents knew that ARVs cannot cure HIV, with those stocking ARVs being more likely to respond correctly (60.3% vs. 34.8%, p = 0.001). Respondents of pharmacies which stocked ARVs were also more likely to believe it was a professional obligation to provide medications to HIV-infected persons (91.8% vs. 78.8%, p = 0.007) but they were also more likely to believe that HIV-infected persons are unable to adhere to their medicines (79.5% vs. 40.9%, p < 0.01). Knowledge of the most common side effects of nevirapine, abnormal liver enzyme profile and skin rash, was reported correctly by 8 (3.9%) and 23 (11.1%) respondents, respectively. Seven (3.4%) respondents reported that they had received special training on HIV, 3 (1.5%) reported receipt of special training on ART and 167 (80.7%) reported that they believed that pharmacy staff should get special training on ART. Conclusion There is a high willingness to participate in HIV management among community-based pharmacies but there is a tremendous need for training on HIV therapies. Furthermore, stigmatizing attitudes towards HIV-infected persons persist and interventions to reduce stigma are needed, particularly among those that stock ARVs.
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- 2010
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16. Raney metal catalysts: I. comparative properties of raney nickel proceeding from Ni-Ai intermetallic phases
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Sane, S., Bonnier, J.M., Damon, J.P., and Masson, J.
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- 1984
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17. Conjunctival dysplasia
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Sane S, Khan N, and Ingole N
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Ophthalmology ,RE1-994 - Published
- 1984
18. A metagenomic assessment of bacterial community in spices sold open-air markets in Saint-Louis, Senegal.
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Sané S, Diouara AAM, Coundoul S, Tene SD, Kane A, Wade SF, Tamba A, Diop M, Mbaye MN, Thiam F, Dieng M, Mbengue M, Nguer CM, Sarr AD, Ndao AS, and Touré Kane C
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- Senegal, Bacteria genetics, Bacteria classification, Bacteria isolation & purification, Humans, Metagenome, Microbiota genetics, Curcuma genetics, Curcuma microbiology, Spices microbiology, Metagenomics methods, RNA, Ribosomal, 16S genetics
- Abstract
Natural spices play an essential role in human nutrition and well-being. However, their processing on different scales can expose them to potential sources of contamination. This study aimed to describe the bacterial community genomic footprint in spices sold in Senegal. Spice samples were collected in August 2022 in Saint-Louis, Senegal. The genomic region coding bacterial 16S rRNA was then amplified and sequenced using Oxford Nanopore Technology (ONT). Sequencing was carried out on two batches of samples, one containing part of the "Local Spices or Herbs" (n = 10), and the other, a mixture of 7 spices, Curcuma, Thyme and the other part of the "Local Spices or Herbs" (n = 39). Results showed high bacterial diversity and the predominance of Escherichia coli and Salmonella enterica in samples, with total reads of 65,744 and 165,325 for the two batches, respectively. The sample category "Homemade mixture of food condiments ", which includes all "Local Spices or Herbs" samples, showed remarkable bacterial diversity. These were followed by Curcuma, a blend of 7 spices and thyme. Also, the different categories of spices studied show similarities in their bacterial composition. These results highlight the microbial community's highly diverse genomic profile, including pathogenic bacteria, in spice samples., (© 2024. The Author(s).)
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- 2024
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19. Detection of Hepatitis E Virus (HEV) in Pork Sold in Saint-Louis, the North of Senegal.
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Tene SD, Diouara AAM, Kane A, Sané S, Coundoul S, Thiam F, Nguer CM, Diop M, Mbaye MN, Mbengue M, Lo S, Diop Ndiaye H, Toure Kane C, and Ayouba A
- Abstract
The hepatitis E virus (HEV) is a zoonotic pathogen with various hosts, including pigs, which act as reservoirs. In industrialized countries, sporadic cases caused by genotype 3, contracted by ingesting contaminated uncooked or undercooked meat, have been reported. However, in developing countries, HEV infection is mainly dominated by genotype 2 and often associated with poor hygiene conditions and drinking water supplies. HEV infection and its circulation in domestic fauna in West Africa are poorly documented. This study aimed to assess the presence of HEV in pork sold in Saint-Louis, Senegal. Meat products (250 g samples, n = 74) were purchased in August 2022 from three locations. Then, 2 g/sample was minced to extract total nucleic acids using the Purelink™ Viral DNA/RNA kit. RT-PCR reactions were performed using the One-Taq™ One-Step RT-PCR kit targeting the HEV ORF2 genomic region. The products obtained were visualized on a 1% agarose gel. Of a total of 74 samples, divided into pork meat ( n = 65) and pork liver ( n = 9), 5.4% ( n = 4) tested positive for HEV. In both cases, two samples were positive, representing a rate of 3.1% and 22.2% for meat and pork liver, respectively. All new viral sequences were obtained from a monophyletic group within HEV genotype 3. This study is the first to report the presence of HEV in pork sold in Senegal and the results reveal a potential circulation of HEV in the pig population. The high proportion of contamination in the pork liver samples highlights a major risk associated with their consumption.
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- 2024
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20. "Whatever journey you want to take, I'll support you through": a mixed methods evaluation of a peer worker program in the hospital emergency department.
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O'Neill M, Michalski C, Hayman K, Hulme J, Dube S, Diemert LM, Kornas K, Schoffel A, Rosella LC, and Boozary A
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- Humans, Ontario, Emergency Service, Hospital, Hospitals, Peer Group, Mental Disorders
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Background: People who are unhoused, use substances (drugs and/or alcohol), and who have mental health conditions experience barriers to care access and are frequently confronted with discrimination and stigma in health care settings. The role of Peer Workers in addressing these gaps in a hospital-based context is not well characterized. The aim of this evaluation was to 1) outline the role of Peer Workers in the care of a marginalized populations in the emergency department; 2) characterize the impact of Peer Workers on patient care, and 3) to describe how being employed as a Peer Worker impacts the Peer., Methods: Through a concurrent mixed methods evaluation, we explore the role of Peer Workers in the care of marginalized populations in the emergency department at two urban hospitals in Toronto, Ontario Canada. We describe the demographic characteristics of patients (n = 555) and the type of supports provided to patients collected through a survey between February and June 2022. Semi-structured, in-depth interviews were completed with Peer Workers (n = 7). Interviews were thematically analyzed using a deductive approach, complemented by an inductive approach to allow new themes to emerge from the data., Results: Support provided to patients primarily consisted of friendly conversations (91.4%), discharge planning (59.6%), tactics to help the patient navigate their emotions/mental wellbeing (57.8%) and sharing their lived experience (50.1%). In over one third (38.9%) of all patient interactions, Peer Workers shared new information about the patient with the health care team (e.g., obtaining patient identification). Five major themes emerged from our interviews with Peer Workers which include: (1) Establishing empathy and building trust between the patient and their care team through self-disclosure; (2) Facilitating a person-centered approach to patient care through trauma-informed listening and accessible language; (3) Support for patient preferences on harm reduction; (4) Peer worker role facilitating self-acceptance and self-defined recovery; and (5) Importance of supports and resources to help Peer Workers navigate the emotional intensity of the emergency department., Conclusions: The findings add to the literature on Peer Worker programs and how such interventions are designed to best meet the needs of marginalized populations., (© 2024. The Author(s).)
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- 2024
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21. The importance of screening for functional neurological disorders in patients with persistent post-concussion symptoms.
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Jobin K, Wang M, du Plessis S, Silverberg ND, and Debert CT
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- Humans, Anxiety diagnosis, Anxiety epidemiology, Anxiety etiology, Surveys and Questionnaires, Post-Concussion Syndrome diagnosis, Post-Concussion Syndrome epidemiology, Post-Concussion Syndrome etiology, Brain Concussion diagnosis, Conversion Disorder complications
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Background: Functional neurological disorder (FND) may commonly co-occur with persistent symptoms following a psychological trauma or physical injury such as concussion., Objective: To explore the occurrence of FND in a population with persistent post-concussion symptoms (PPCS) and the associations between FND and depression as well as anxiety in participants with PPCS., Methods: Sixty-three individuals with PPCS presenting to a specialized brain injury clinic completed the following questionnaires: screening for somatoform disorder conversion disorder subscale (SOM-CD), Rivermead post-concussion symptom questionnaire (RPQ), patient health questionnaire-9 (PHQ-9), and generalized anxiety disorder questionnaire- 7 (GAD-7). Both multiple linear regression and logistic regression were conducted to evaluate the relationship between questionnaires and adjust for covariates., Results: We found that total RPQ score (βˆ= 0.27; 95% CI = [0.16, 0.38]), GAD-7 score (βˆ= 0.71; 95% CI = [0.50, 0.92]) and PHQ-9 score (βˆ= 0.54; 95% CI = [0.32, 0.76]) were positively associated with SOM-CD score individually, after consideration of other covariates. Participants meeting the criteria for severe FND symptoms were 4.87 times more likely to have high PPCS symptom burden (95% CI = [1.57, 22.84]), 8.95 times more likely to have severe anxiety (95% CI = [3.31, 35.03]) and 4.11 times more likely to have severe depression symptom burden (95% CI = [1.77, 11.53])., Conclusion: The findings of this study indicate an association between FND and post-concussion symptoms as well as an association between FND and symptoms of depression and anxiety in patients with PPCS. Patients with PPCS should be screened for FND to provide a more targeted treatment approach that includes somatic-focused interventions.
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- 2023
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22. Can the Healthcare System Improve Food Security? A Need for Collaborative Community Partnerships.
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Wiens K, O'Neill M, Redelmeier RJ, Wali S, Chagpar A, Dube S, Diemert L, Michalski C, Ziebell B, Elliott S, Campbell L, Rosella L, and Boozary A
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- Delivery of Health Care, Food Security, Food Supply, Humans, COVID-19 epidemiology, COVID-19 prevention & control, Pandemics
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The COVID-19 pandemic has heightened the food insecurity crisis in Canada, and existing supports have been largely insufficient to meet the food needs of communities. In response to increasing reports of food insecurity among Toronto residents during the pandemic, the Food RX program was developed as a collaborative initiative between FoodShare Toronto - a local, community-based food justice organization - and the University Health Network, a large university-affiliated hospital network in downtown Toronto, ON. This commentary describes the Food RX program, highlights the lessons learned during its early implementation and offers a set of recommendations for building community partnerships moving forward., (Copyright © 2022 Longwoods Publishing.)
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- 2022
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23. Treatment of Persistent Postconcussion Syndrome With Repetitive Transcranial Magnetic Stimulation Using Functional Near-Infrared Spectroscopy as a Biomarker of Response: Protocol for a Randomized Controlled Clinical Trial.
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du Plessis S, Oni IK, Lapointe AP, Campbell C, Dunn JF, and Debert CT
- Abstract
Background: Approximately one-third of all concussions lead to persistent postconcussion syndrome (PPCS). Repetitive transcranial magnetic stimulation (rTMS) is a form of noninvasive brain stimulation that has been extensively used to treat refractory major depressive disorder and has a strong potential to be used as a treatment for patients with PPCS. Functional near-infrared spectroscopy (fNIRS) has already been used as a tool to assess patients with PPCS and may provide insight into the pathophysiology of rTMS treatment in patients with PPCS., Objective: The primary objective of this research is to determine whether rTMS treatment improves symptom burden in patients with PPCS compared to sham treatment using the Rivermead postconcussion symptom questionnaire. The secondary objective is to explore the neuropathophysiological changes that occur following rTMS in participants with PPCS using fNIRS. Exploratory objectives include determining whether rTMS treatment in participants with PPCS will also improve quality of life, anxiety, depressive symptoms, cognition, posttraumatic stress, and function secondary to headaches., Methods: A total of 44 adults (18-65 years old) with PPCS (>3 months to 5 years) will participate in a double-blind, sham-controlled, concealed allocation, randomized clinical trial. The participants will engage in either a 4-week rTMS treatment protocol or sham rTMS protocol (20 treatments). The left dorsolateral prefrontal cortex will be located through Montreal Neurologic Institute coordinates. The intensity of the rTMS treatment over the left dorsolateral prefrontal cortex will be 120% of resting motor threshold, with a frequency of 10 Hz, 10 trains of 60 pulses per train (total of 600 pulses), and intertrain interval of 45 seconds. Prior to starting the rTMS treatment, participant and injury characteristics, questionnaires (symptom burden, quality of life, depression, anxiety, cognition, and headache), and fNIRS assessment will be collected. Repeat questionnaires and fNIRS will occur immediately after rTMS treatment and at 1 month and 3 months post rTMS. Outcome parameters will be analyzed by a 2-way (treatment × time) mixed analysis of variance., Results: As of May 6, 2021, 5 participants have been recruited for the study, and 3 have completed the rTMS protocol. The estimated completion date of the trial is May 2022., Conclusions: This trial will expand our knowledge of how rTMS can be used as a treatment option of PPCS and will explore the neuropathophysiological response of rTMS through fNIRS analysis., Trial Registration: ClinicalTrials.gov NCT04568369; https://clinicaltrials.gov/ct2/show/NCT04568369., International Registered Report Identifier (irrid): DERR1-10.2196/31308., (©Sané du Plessis, Ibukunoluwa K Oni, Andrew P Lapointe, Christina Campbell, Jeff F Dunn, Chantel T Debert. Originally published in JMIR Research Protocols (https://www.researchprotocols.org), 22.03.2022.)
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- 2022
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24. In it to win it: Competitiveness, concussion knowledge and nondisclosure in athletes.
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Doucette MM, Du Plessis S, Webber AM, Whalen C, and Garcia-Barrera MA
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- Adolescent, Adult, Female, Humans, Male, Young Adult, Athletes, Canada, Self Disclosure, Intention, Health Knowledge, Attitudes, Practice, Athletic Injuries, Brain Concussion, Sports
- Abstract
Objectives: Despite having a general understanding of concussions, many athletes choose not to report symptoms of a sports-related concussion (SRC) which leads to a larger burden on our healthcare system due to longer recoveries, more Emergency Department visits, and future medical appointments. Thus, there is a need to identify factors, such as competitiveness, that may help to better explain the nondisclosure of concussion symptoms. The current study aimed to investigate the role of athletes' concussion knowledge and competitiveness on concussion nondisclosure., Methods: Participants included 161 Canadian athletes (ages 14-32; 71% male) participating in collision sports (American football, rugby, hockey), soccer or rowing. Concussion knowledge, reporting and competitiveness were measured using the Rosenbaum Concussion Attitude and Knowledge Survey and Sports Orientation Questionnaire (SOQ). Two logistic regression models were performed to explain both history and future intention of playing despite experiencing concussion symptoms., Results: Age, sex, and concussion knowledge were not significant for the regression models. The SOQ scores (measuring competitiveness) were significant for both regression models, such that higher competitiveness was associated with increased likelihood for past nondisclosure ( b =.03, p =.03, OR = 1.03 (97.5% CI: 1.003,1.06)) and future nondisclosure intention ( b =.05, p =.00, OR = 1.05 (97.5% CI: 1.03,1.08)). Regarding concussion knowledge, soccer players had significantly higher knowledge than those in collision sports and rugby, F (2, 158) = 140.5, p =.00; female athletes had significantly higher knowledge than males, t (66) = -4.26, p =.00; age was negatively associated with concussion knowledge ( r = -0.35, p < .01)., Conclusion: The results suggest that healthcare providers should be aware that athletes tend to have adequate concussion knowledge, but this knowledge does not explain past nondisclosure or future nondisclosure intention. Based on these findings, healthcare providers should focus on being able to identify highly competitive athletes who are most at risk of nondisclosure instead of exclusively aiming to increase concussion knowledge in athletes.
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- 2021
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25. Measurement of transepidermal water loss, stratum corneum hydration and skin surface pH in occupational settings: A review.
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Jansen van Rensburg S, Franken A, and Du Plessis JL
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- Agriculture, Body Water physiology, Humans, Hydrogen-Ion Concentration, Industry, Nursing, Printing, Skin Physiological Phenomena, Epidermis physiology, Occupational Diseases physiopathology, Organism Hydration Status physiology, Skin Diseases physiopathology, Water Loss, Insensible physiology
- Abstract
Background: The skin provides a permeable barrier which may be impaired in occupational settings. Transepidermal water loss (TEWL), stratum corneum hydration (SCH) and skin surface pH (SSpH) have been used in occupational settings to predict early onset of occupational skin diseases, to measure the effectiveness of prevention strategies for occupational skin diseases, and to assess skin condition during exposure. The aim was to compare the findings, identify shortcomings in the methodology and data reporting and furthermore, to make recommendations for future studies., Materials and Methods: A literature study was conducted on studies published before December 2018 to provide a review on the measurement of TEWL, SCH and SSpH in occupational settings., Results: TEWL, SCH and SSpH were previously measured in a wide variety of industries. Results between studies were highly variable, due to different study designs and different anatomical positions measured. Not all of the measurement conditions were reported and variations in study objectives led to data being reported and interpreted differently for most studies., Conclusion: Incomplete reporting of methodology hinders comparison of bioengineering measurements. No bioengineering method has been proven useful as a predictive tool for occupational skin diseases, however, it is useful in the acute assessment of skin condition. It is recommended that future studies on TEWL, SCH and SSpH adhere to guidelines for occupational settings as far as possible to enable comparison between studies., (© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
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- 2019
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26. An air-breathing enzymatic cathode with extended lifetime by continuous laccase supply.
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Kipf E, Sané S, Morse D, Messinger T, Zengerle R, and Kerzenmacher S
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- Electricity, Electrodes, Trametes, Bioelectric Energy Sources, Laccase metabolism
- Abstract
We present a novel concept of an air-breathing enzymatic biofuel cell cathode combined with continuous supply of unpurified laccase-containing supernatant of the white-rot fungus Trametes versicolor for extended lifetime. The air-breathing cathode design obviates the need for energy-intensive active aeration. In a corresponding long-term experiment at a constant current density of 50 µA cm
-2 , we demonstrated an increased lifetime of 33 days (cathode potential above 0.430 V vs. SCE), independent of enzyme degradation. The obtained data suggest that theoretically a longer lifetime is feasible. However, further engineering efforts are required to prevent clogging and fouling of the supply tubes. These results represent an important step towards the realization of enzymatic biofuel cell cathodes with extended lifetime and enhanced performance., (Copyright © 2018 Elsevier Ltd. All rights reserved.)- Published
- 2018
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27. The influence of pH on the in vitro permeation of rhodium through human skin.
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Jansen Van Rensburg S, Franken A, Du Plessis J, and Du Plessis JL
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- Adult, Environmental Exposure, Female, Humans, In Vitro Techniques, Skin drug effects, Skin metabolism, White People, Hydrogen-Ion Concentration, Rhodium metabolism, Skin Absorption drug effects
- Abstract
Workers in precious metals refineries are at risk of exposure to salt compounds of the platinum group metals through inhalation, as well as through the skin. Rhodium salt permeation through the skin has previously been proven using rhodium trichloride (RhCl
3 ) dissolved in synthetic sweat at a pH of 6.5. However, the skin surface pH of refinery workers may be lower than 6.5. The aim of this study was to investigate the influence of pH 6.5 and 4.5 on the in vitro permeation of rhodium through intact Caucasian skin using Franz diffusion cells. A concentration of 0.3 mg mL-1 rhodium was used and analyses were performed using inductively coupled plasma mass spectrometry and inductively coupled plasma optical emission spectrometry. Results indicated a cumulative increase in permeation over 24 h. Rhodium permeation after 12 h was significantly greater at pH 4.5 (1.56 ± 0.24 ng cm-2 ) than at 6.5 (0.85 ± 0.13 ng cm-2 ; p = 0.02). At both pH levels, there was a highly significant difference ( p < 0.01) between the mass of rhodium remaining in the skin (1428.68 ± 224.67 ng cm-2 at pH 4.5 and 1029.90 ± 115.96 ng cm-2 at pH 6.5) and the mass that diffused through (0.88 ± 0.17 ng cm-2 at pH 4.5 and 0.62 ± 0.10 ng cm-2 at pH 6.5). From these findings, it is evident that an acidic working environment or low skin surface pH may enhance permeation of rhodium salts, contributing to sensitization and adverse health effects.- Published
- 2017
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28. Using planktonic microorganisms to supply the unpurified multi-copper oxidases laccase and copper efflux oxidases at a biofuel cell cathode.
- Author
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Sané S, Richter K, Rubenwolf S, Matschke NJ, Jolivalt C, Madzak C, Zengerle R, Gescher J, and Kerzenmacher S
- Subjects
- Base Sequence, DNA Primers, Escherichia coli metabolism, Yarrowia metabolism, Bioelectric Energy Sources, Electrodes, Oxidoreductases metabolism, Plankton metabolism
- Abstract
The feasibility to apply crude culture supernatants that contain the multicopper oxidases laccase or copper efflux oxidase (CueO) as oxygen reducing catalysts in a biofuel cell cathode is shown. As enzyme-secreting recombinant planktonic microorganisms, the yeast Yarrowia lipolytica and the bacterium Escherichia coli were investigated. The cultivation and operation conditions (choice of medium, pH) had distinct effects on the electro-catalytic performance. The highest current density of 119 ± 23 μA cm(-2) at 0.400 V vs. NHE was obtained with the crude culture supernatant of E. coli cells overexpressing CueO and tested at pH 5.0. In comparison, at pH 7.4 the electrode potential at 100 μA cm(-2) is 0.25 V lower. Laccase-containing supernatants of Y. lipolytica yielded a maximum current density of 6.7 ± 0.4 μAcm(-2) at 0.644 V vs. NHE. These results open future possibilities to circumvent elaborate enzyme purification procedures and realize cost effective and easy-to-operate enzymatic biofuel cells., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
29. Overcoming bottlenecks of enzymatic biofuel cell cathodes: crude fungal culture supernatant can help to extend lifetime and reduce cost.
- Author
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Sané S, Jolivalt C, Mittler G, Nielsen PJ, Rubenwolf S, Zengerle R, and Kerzenmacher S
- Subjects
- Electrodes, Hydrogen-Ion Concentration, Time Factors, Bioelectric Energy Sources economics, Bioelectric Energy Sources microbiology, Culture Media, Conditioned metabolism, Culture Techniques, Laccase metabolism, Trametes enzymology, Trametes growth & development
- Abstract
Enzymatic biofuel cells (BFCs) show great potential for the direct conversion of biochemically stored energy from renewable biomass resources into electricity. However, enzyme purification is time-consuming and expensive. Furthermore, the long-term use of enzymatic BFCs is hindered by enzyme degradation, which limits their lifetime to only a few weeks. We show, for the first time, that crude culture supernatant from enzyme-secreting microorganisms (Trametes versicolor) can be used without further treatment to supply the enzyme laccase to the cathode of a mediatorless BFC. Polarization curves show that there is no significant difference in the cathode performance when using crude supernatant that contains laccase compared to purified laccase in culture medium or buffer solution. Furthermore, we demonstrate that the oxygen reduction activity of this enzymatic cathode can be sustained over a period of at least 120 days by periodic resupply of crude culture supernatant. This is more than five times longer than control cathodes without the resupply of culture supernatant. During the operation period of 120 days, no progressive loss of potential is observed, which suggests that significantly longer lifetimes than shown in this work may be possible. Our results demonstrate the possibility to establish simple, cost efficient, and mediatorless enzymatic BFC cathodes that do not require expensive enzyme purification procedures. Furthermore, they show the feasibility of an enzymatic BFC with an extended lifetime, in which self-replicating microorganisms provide the electrode with catalytically active enzymes in a continuous or periodic manner., (Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2013
- Full Text
- View/download PDF
30. Phytoplankton growth control and risk of cyanobacterial blooms in the lower Senegal River delta region.
- Author
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Quiblier C, Leboulanger C, Sané S, and Dufour P
- Subjects
- Biomass, Chelating Agents pharmacology, Cyanobacteria drug effects, Edetic Acid pharmacology, Fresh Water, Metals pharmacology, Phytoplankton drug effects, Seawater, Senegal, Cyanobacteria growth & development, Eutrophication, Nitrogen pharmacology, Phosphorus pharmacology, Phytoplankton growth & development
- Abstract
Eutrophication, and the resulting proliferation of phytoplankton can affect water quality. Although environmental and ecological processes that lead to phytoplankton proliferations in temperate areas have received considerable attention, scientific background regarding this topic in tropical areas, especially West Africa, are scarce. In this study, bioassays in batch cultures were carried out in order to identify factors that may be stimulating or limiting the biomass of phytoplankton at various sites in the lower Senegal River delta region. Complete factorial designs were used to test several factors both alone and combined (nitrogen, phosphorus, sulfate, EDTA and trace elements, stirring). The effects of these factors on the growth of the whole phytoplankton communities were followed by the daily measurement of in vivo fluorescence. At all but two of the sites, enrichment with certain nutrients significantly stimulated phytoplankton biomass growth, with nitrogen and/or phosphorus appearing to be the primary limiting factor(s). Nitrogen was the primary limiting factor in coastal ocean waters and in the Senegal River estuary. In the freshwater ecosystems studied, nitrogen and phosphorus acted as alternating or concomitant limiting factors. We assessed the changes in the phytoplankton composition induced by nutrient enrichments in Lake Guiers, a drinking water reservoir for the population of Dakar. Our findings lead us to define the conditions of a possible proliferation of the potentially toxic cyanobacterium Cylindrospermopsis raciborskii mainly in response to phosphorus inputs.
- Published
- 2008
- Full Text
- View/download PDF
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