1. Efficient synthesis of the 2-aminoimidazolium-bridged diguanosyl intermediate for nonenzymatic primer extension.
- Author
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Sammons, Scott R., Robinson, James D., and O'Flaherty, Derek K.
- Subjects
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CHEMICAL processes , *ORIGIN of life , *OXIDATION-reduction reaction , *HOMODIMERS , *SOLUBILITY - Abstract
Nonenzymatic genome replication has yet to be demonstrated experimentally. Recently, studies on the nonenzymatic primer extension mechanism mediated by nucleoside 5′-monophosphates (NMPs) activated with 2-aminoimidazole (2AI) have revealed that imidazolium-bridged dinucleotide intermediates (N*N) are responsible for the bulk of the chemical copying process. As a result, an efficacious synthetic pathway for producing these substrates is desirable. Standard dehydrative redox reaction between NMPs and 2AI can produce N*N in high yields for all homodimers, apart from riboguanosine 5′-monophosphate. Here, we report a vastly improved methodology for the preparation of G*G, by the introduction of a simple reverse-phase cation exchange step to increase the solubility of the mononucleotide. Our approach is more efficient, cost-effective, less time-consuming, and has better atom economy than the currently established methodology for this important molecule in primer extension studies. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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