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Citrullus colocynthis L. of the Cucurbitaecea botanical family is a plant widely used in traditional medicine in Algeria. It seems empirically effective in the treatment of various diseases. The aim of the present research is to evaluate the antioxidant and cytotoxic effects, in vitro, of the ethanolic extract of C. colocynthis fruits. The quantitative analysis has shown that the ethanolic extract of C. colocynthis is rich in total polyphenols with a content of 443.62 ± 2.13 µg EAG/mg of extract. The results obtained showed a strong anti-free radical activity of the ethanolic extract of C.colocynthis exerted against the DPPH free radical scavenging effect (CI50 = 6.31 µg/ml) and highlighted a powerful ferric reducing antioxidant power (CI50 = 27.94 µg/ml). We should also note a good antioxidant activity against the OH radical, obtained with the concentration (IC50 = 67.13 µg/ml). Furthermore, the obtained results indicate that the treatment of the three cancer cell lines (HepG2, SH-SY5Y and Raw 264.7) with the different concentrations of the used extract reduced the number of cells in a dose-dependent way. Based on our results, we can consider that Citrullus colocynthis is a plant with a strong pharmacological power and can therefore be used in phytotherapy.

In an aim to prove the efficiency of polyphenols of Rosa canina fruits in promoting human health. A methanolic extract of R. canina fruits was prepared by successive maceration with solvents of increasing polarity. The polyphenol composition was analyzed by HPLC-DAD-ESI-MS. The biological activity of this extract on SH-SY5Y cells and HepG2 cells was then studied. The antioxidant activity was tested by various in vitro tests such as DPPH-radical-scavenging activity, FRAP assay, hydroxyl radical scavenging assay and total antioxidant capacity. The subacute toxicity of R. canina was tested on female rats by repeated intraperitoneal administration of various doses. The phenolic profiles showed 25 antioxidants distributed into three classes of phenolic compounds: glycosylated and agglomerated flavonoids/isoflavonoids, tannins and phenanthrenes. Qualitative phytochemical analyses showed that this extract lacks alkaloids. The methanolic extract of R. canina fruits has a total antioxidant capacity of 82.69 ± 1.18 μg EAA/mg of methanol extract and the IC50 of the methods used is in the following increasing order: FRAP assay (61.88 μg/ml), then hydroxyl radical scavenging assay (67.45 μg/ml) and then DPPH radical-scavenging activity (129.81 μg/ml). The extract of R. canina did not cause any phenotypic signs of toxicity or mortality during and after treatment. The LD50 was >5,000 mg/kg, hence, R. canina was considered nontoxic. An in vivo study proved the protective effect of R. canina against cardiac and hepato-renal toxicities. These results drew the importance of a healthy diet, where diets rich in R. canina fruits can be used as a rich natural source of antioxidants and anticarcinogenic phenolic compounds.

The present study was carried out in order to identify and characterize the compounds of Rosa canina fruits by HPLC-UV-MS. The total phenolic determiner by a new Fast Blue method (FBBB), which detects phenolic directly, reported an average total phenolic concentration of 1.7 folds greater than Folin-Ciocalteu (F-C), which indicates that an indirect detection method of total phenolic should be replaced in future studies by the FBBB method. TPC of the ethanolic extract was positively correlated with 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging effect. The DPPH activity of R. canina extract which is higher than the IC50 of the ascorbic acid and Butylated Hydroxytoluene (BHT), but lower than the IC50 of quercetin and trolox. The determination of intracellular reactive oxygen species (ROS) proved the antioxidant effect of the extract on HepG2 and SH-SY5Y cells. A concentration of 1.63 μg/ml on HepG2 cells had an oxidizing effect instead of the antioxidant effect, which is due to the existence of a tert-butyl group in sesquiterpene identified by HPLC-UV-MS method. These results indicate that the fruits of R. canina can be used as a natural source of antioxidants against oxidative stress and some types of cancer.

Introduction-Objectif-Dans les dernières décennies il y a eu un intérêt croissant pour l’étude des plantes médicinales et leur utilisation traditionnelle pour le traitement de diverses maladies. L’olivier ou Olea europaea L. constitue une entité indissociable des peuples méditerranéens. Cette plante appartient à la grande famille des oleaceae. L’objectif de cette étude a été d’évaluer l’activité anti-oxydante des extraits éthanoliques de quatre variétés sauvages de la plante O.europaea des régions de l’Est algérien : Batna et Mila (hautes-plateaux), Biskra (Sahara) et Skikda (ville côtière). Matériels et méthodes - L’évaluation de l’activité anti-oxydante in vitro des extraits éthanoliques de la plante O.europaea a été réalisée par différentes méthodes, notamment le piégeage du radical libre DPPH• et le pouvoir réducteur des ions ferriques. Résultats -L’analyse quantitative des extraits éthanoliques des feuilles de la plante O.europaea a révélé que l’extrait de Mila était plus riche (420.36 mg GAE/g MS), suivi de celui de Batna (396.84 mg GAE/g MS), puis celui de Biskra (de 380.69 mg GAE/g MS) et enfin de l’extrait de Skikda (368.45 mg GAE/g MS). Les résultats de l’activité antiradicalaire des extraits éthanoliques ont montré que ceux de Batna et Mila possédaient le pouvoir le plus important. Conclusion -Les résultats obtenus confirment favorablement l’utilisation des feuilles d’O.europaea en médecine traditionnelle algérienne pour le traitement de diverses maladies.