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Your search keyword '"Sambucci, Manolo"' showing total 33 results
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33 results on '"Sambucci, Manolo"'

1. Genetically Driven CD39 Expression Affects Sezary Cell Viability and IL-2 Production and Detects Two Patient Subsets with Distinct Prognosis

Author

Picozza, Mario, Cristofoletti, Cristina, Bresin, Antonella, Fioretti, Martina, Sambucci, Manolo, Scala, Enrico, Monopoli, Alessandro, Cantonetti, Maria, Pilla, Maria Antonietta, Accetturi, Maria Pina, Borsellino, Giovanna, D’Atri, Stefania, Caprini, Elisabetta, Russo, Giandomenico, and Narducci, Maria Grazia

Published
2022
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2. Rafoxanide negatively modulates STAT3 and NF‐κB activity and inflammation‐associated colon tumorigenesis.

Author

Pacifico, Teresa, Stolfi, Carmine, Tomassini, Lorenzo, Luiz‐Ferreira, Anderson, Franzè, Eleonora, Ortenzi, Angela, Colantoni, Alfredo, Sica, Giuseppe S., Sambucci, Manolo, Monteleone, Ivan, Monteleone, Giovanni, and Laudisi, Federica

Abstract

In the colorectal cancer (CRC) niche, the transcription factors signal transducer and activator of transcription 3 (STAT3) and nuclear factor‐κB (NF‐κB) are hyperactivated in both malignant cells and tumor‐infiltrating leukocytes (TILs) and cooperate to maintain cancer cell proliferation/survival and drive protumor inflammation. Through drug repositioning studies, the anthelmintic drug rafoxanide has recently emerged as a potent and selective antitumor molecule for different types of cancer, including CRC. Here, we investigate whether rafoxanide could negatively modulate STAT3/NF‐κB and inflammation‐associated CRC. The antineoplastic effect of rafoxanide was explored in a murine model of CRC resembling colitis‐associated disease. Cell proliferation and/or STAT3/NF‐κB activation were evaluated in colon tissues taken from mice with colitis‐associated CRC, human CRC cells, and CRC patient‐derived explants and organoids after treatment with rafoxanide. The STAT3/NF‐κB activation and cytokine production/secretion were assessed in TILs isolated from CRC specimens and treated with rafoxanide. Finally, we investigated the effects of TIL‐derived supernatants cultured with or without rafoxanide on CRC cell proliferation and STAT3/NF‐κB activation. The results showed that rafoxanide restrains STAT3/NF‐κB activation and inflammation‐associated colon tumorigenesis in vivo without apparent effects on normal intestinal cells. Rafoxanide markedly reduces STAT3/NF‐κB activation in cultured CRC cells, CRC‐derived explants/organoids, and TILs. Finally, rafoxanide treatment impairs the ability of TILs to produce protumor cytokines and promote CRC cell proliferation. We report the novel observation that rafoxanide negatively affects STAT3/NF‐κB oncogenic activity at multiple levels in the CRC microenvironment. Our data suggest that rafoxanide could potentially be deployed as an anticancer drug in inflammation‐associated CRC. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Phenformin Inhibits Hedgehog-Dependent Tumor Growth through a Complex I-Independent Redox/Corepressor Module

Author

Di Magno, Laura, Manni, Simona, Di Pastena, Fiorella, Coni, Sonia, Macone, Alberto, Cairoli, Sara, Sambucci, Manolo, Infante, Paola, Moretti, Marta, Petroni, Marialaura, Nicoletti, Carmine, Capalbo, Carlo, De Smaele, Enrico, Di Marcotullio, Lucia, Giannini, Giuseppe, Battistini, Luca, Goffredo, Bianca Maria, Iorio, Egidio, Agostinelli, Enzo, Maroder, Marella, and Canettieri, Gianluca

Published
2020
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4. Primary and Recall Immune Responses to SARS-CoV-2 in Breakthrough Infection

Author

D’Orso, Silvia, primary, Pirronello, Marta, additional, Verdiani, Alice, additional, Rossini, Angelo, additional, Guerrera, Gisella, additional, Picozza, Mario, additional, Sambucci, Manolo, additional, Misiti, Andrea, additional, De Marco, Lorenzo, additional, Salvia, Antonino, additional, Caltagirone, Carlo, additional, Giardina, Emiliano, additional, Battistini, Luca, additional, and Borsellino, Giovanna, additional

Published
2023
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6. Primary and Recall Immune Responses to SARS-CoV-2 in Breakthrough Infection.

Author

D'Orso, Silvia, Pirronello, Marta, Verdiani, Alice, Rossini, Angelo, Guerrera, Gisella, Picozza, Mario, Sambucci, Manolo, Misiti, Andrea, De Marco, Lorenzo, Salvia, Antonino, Caltagirone, Carlo, Giardina, Emiliano, Battistini, Luca, and Borsellino, Giovanna

Subjects
BREAKTHROUGH infections, MEDICAL personnel, IMMUNE response, SARS-CoV-2, B cells
Abstract

Breakthrough infections in SARS-CoV-2 vaccinated individuals are an ideal circumstance for the simultaneous exploration of both the vaccine-induced memory reaction to the spike (S) protein and the primary response to the membrane (M) and nucleocapsid (N) proteins generated by natural infection. We monitored 15 healthcare workers who had been vaccinated with two doses of Pfizer BioNTech BNT162b2 and were then later infected with the SARS-CoV-2 B.1.617.2. (Delta) variant, analysing the antiviral humoral and cellular immune responses. Natural infection determined an immediate and sharp rise in anti-RBD antibody titres and in the frequency of both S-specific antibody secreting cells (ASCs) and memory B lymphocytes. T cells responded promptly to infection by activating and expanding already at 2–5 days. S-specific memory and emerging M- and N-specific T cells both expressed high levels of activation markers and showed effector capacity with similar kinetics but with different magnitude. The results show that natural infection with SARS-CoV-2 in vaccinated individuals induces fully functional and rapidly expanding T and B lymphocytes in concert with the emergence of novel virus-specific T cells. This swift and punctual response also covers viral variants and captures a paradigmatic case of a healthy adaptive immune reaction to infection with a mutating virus. [ABSTRACT FROM AUTHOR]

Published
2023
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8. Assessment of T-cell Reactivity to the SARS-CoV-2 Omicron Variant by Immunized Individuals

Author

De Marco, Lorenzo, primary, D’Orso, Silvia, additional, Pirronello, Marta, additional, Verdiani, Alice, additional, Termine, Andrea, additional, Fabrizio, Carlo, additional, Capone, Alessia, additional, Sabatini, Andrea, additional, Guerrera, Gisella, additional, Placido, Roberta, additional, Sambucci, Manolo, additional, Angelini, Daniela F., additional, Giannessi, Flavia, additional, Picozza, Mario, additional, Caltagirone, Carlo, additional, Salvia, Antonino, additional, Volpe, Elisabetta, additional, Balice, Maria Pia, additional, Rossini, Angelo, additional, Rötzschke, Olaf, additional, Giardina, Emiliano, additional, Battistini, Luca, additional, and Borsellino, Giovanna, additional

Published
2022
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11. BNT162b2 vaccination induces durable SARS-CoV-2–specific T cells with a stem cell memory phenotype

Author

Guerrera, Gisella, primary, Picozza, Mario, additional, D’Orso, Silvia, additional, Placido, Roberta, additional, Pirronello, Marta, additional, Verdiani, Alice, additional, Termine, Andrea, additional, Fabrizio, Carlo, additional, Giannessi, Flavia, additional, Sambucci, Manolo, additional, Balice, Maria Pia, additional, Caltagirone, Carlo, additional, Salvia, Antonino, additional, Rossini, Angelo, additional, Battistini, Luca, additional, and Borsellino, Giovanna, additional

Published
2021
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13. The BNT162b2 mRNA vaccine induces polyfunctional T cell responses with features of longevity.

Author

Borsellino, Giovanna, primary, Guerrera, Gisella, additional, Picozza, Mario, additional, D'Orso, Silvia, additional, Placido, Roberta, additional, Pirronello, Marta, additional, Verdiani, Alice, additional, Termine, Andrea, additional, Fabrizio, Carlo, additional, Giannessi, Flavia, additional, Sambucci, Manolo, additional, Balice, Maria Pia, additional, Caltagirone, Carlo, additional, Salvia, Antonino, additional, Rossini, Angelo, additional, and Battistini, Luca, additional

Published
2021
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19. Impaired intracortical transmission in G2019S leucine rich-repeat kinase Parkinson patients

Author

Ponzo, Viviana, Di Lorenzo, Francesco, Brusa, Livia, Schirinzi, Tommaso, Battistini, Stefania, Ricci, Claudia, Sambucci, Manolo, Caltagirone, Carlo, and Koch, Giacomo

Subjects
Cerebral Cortex, LRRK2, Parkinson disease, TMS, cortical plasticity, levodopa, Aged, Antiparkinson Agents, Case-Control Studies, Cerebral Cortex, Leucine-Rich Repeat Serine-Threonine Protein Kinase-2, Long-Term Potentiation, Motor Cortex, Neural Inhibition, Neuronal Plasticity, Neural Pathways,Parkinson Disease, Synaptic Transmission, Transcranial Magnetic Stimulation, gamma-Aminobutyric Acid, Neuronal Plasticity, Long-Term Potentiation, Motor Cortex, cortical plasticity, Neural Inhibition, LRRK2, Leucine-Rich Repeat Serine-Threonine Protein Kinase-2, Synaptic Transmission, Transcranial Magnetic Stimulation, NO, Antiparkinson Agents, Parkinson disease, Neurology, Case-Control Studies, Cortical plasticity, Levodopa, TMS, Neurology (clinical), Neural Pathways, levodopa, Settore MED/26 - Neurologia, gamma-Aminobutyric Acid, Aged
Published
2017

20. FoxP3 isoforms and PD-1 expression by T regulatory cells in multiple sclerosis

Author

Sambucci, Manolo, primary, Gargano, Francesca, additional, De Rosa, Veronica, additional, De Bardi, Marco, additional, Picozza, Mario, additional, Placido, Roberta, additional, Ruggieri, Serena, additional, Capone, Alessia, additional, Gasperini, Claudio, additional, Matarese, Giuseppe, additional, Battistini, Luca, additional, and Borsellino, Giovanna, additional

Published
2018
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31. Genetically driven CD39 expression affects Sezary cell viability, IL-2 production and detects two patient subsets with distinct prognosis

Author

Picozza, Mario, Cristofoletti, Cristina, Bresin, Antonella, Fioretti, Martina, Sambucci, Manolo, Scala, Enrico, Monopoli, Alessandro, Cantonetti, Maria, Pilla, Maria Antonietta, Accetturi, Maria Pina, Borsellino, Giovanna, D’Atri, Stefania, Caprini, Elisabetta, Russo, Giandomenico, and Narducci, Maria Grazia

Abstract

Sezary Syndrome (SS) is a rare and aggressive variant of Cutaneous T-Cell Lymphoma. It is characterized by the co-presence of CD4+ neoplastic lymphocytes, named Sezary cells, mainly in the blood, lymph-nodes and skin where they induce chronic inflammation which in turn impairs patients’quality of life and fuels neoplastic cells. No available treatment is resolutive for SS but, immunotherapy is becoming an effective option for this lymphoma.Here, we investigated, in a large cohort of SS patients, expression and function of the immune-checkpoint molecule CD39, which degrades pro-inflammatory extracellular (e)ATP.

Published
2022
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32. FoxP3 isoforms and PD-1 expression by T regulatory cells in multiple sclerosis

Author

Serena Ruggieri, Manolo Sambucci, Giuseppe Matarese, Alessia Capone, Giovanna Borsellino, Luca Battistini, Francesca Gargano, Roberta Placido, Marco De Bardi, Veronica De Rosa, Mario Picozza, Claudio Gasperini, Sambucci, Manolo, Gargano, Francesca, De Rosa, Veronica, De Bardi, Marco, Picozza, Mario, Placido, Roberta, Ruggieri, Serena, Capone, Alessia, Gasperini, Claudio, Matarese, Giuseppe, Battistini, Luca, and Borsellino, Giovanna

Subjects
0301 basic medicine, Gene isoform, Adult, Male, Multiple Sclerosis, FoxP3, PD-1 expression, T regulatory cells, multiple sclerosis, Immunoblotting, Programmed Cell Death 1 Receptor, lcsh:Medicine, chemical and pharmacologic phenomena, Disease, Biology, T-Lymphocytes, Regulatory, Article, Flow cytometry, law.invention, 03 medical and health sciences, 0302 clinical medicine, law, medicine, Humans, Protein Isoforms, Immune homeostasis, lcsh:Science, Cells, Cultured, Autoimmune disease, Multidisciplinary, medicine.diagnostic_test, Multiple sclerosis, lcsh:R, FOXP3, Forkhead Transcription Factors, hemic and immune systems, Middle Aged, medicine.disease, Flow Cytometry, 3. Good health, Cell biology, 030104 developmental biology, Suppressor, lcsh:Q, Female, 030217 neurology & neurosurgery
Abstract

Forkhead box P3 (FoxP3)+ regulatory T cells (Treg) are powerful mediators of immune regulation and immune homeostasis. In humans, Tregs are a heterogeneous population expressing surface markers which define phenotypically and functionally distinct subsets. Moreover, it is now clear that intracellular staining for FoxP3 does not unequivocally identify “true” suppressor cells, since several FoxP3 isoforms exist, and different reagents for FoxP3 detection are available. Here, we propose a strategy to identify potentially functional and suppressive Treg cells in an autoimmune disease like multiple sclerosis, and we suggest that in patients affected by this disease these cells are both reduced in number and functionally exhausted.

Published
2018
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33. Increased Foxp3+ Regulatory T Cells in Poly(ADP-Ribose) Polymerase-1 Deficiency

Author

Manolo Sambucci, Maria Manuela Rosado, Claudio Pioli, Federica Laudisi, Francesca Nasta, Pioli, Claudio, Rosado, Maria Manuela, Sambucci, Manolo, Laudisi, Federica, and Nasta, Francesca

Subjects
Regulatory T cell differentiation, lymphocyte differentiation, Immunology, lymphocyte differentiation, regulatory T cells, PARP-1, Immunology, Poly (ADP-Ribose) Polymerase-1, PARP-1, Cell Separation, Thymus Gland, T-Lymphocytes, Regulatory, regulatory T cells, TCIRG1, Mice, Interleukin 21, T-Lymphocyte Subsets, Animals, Immunology and Allergy, Cytotoxic T cell, IL-2 receptor, Mice, Knockout, CD40, biology, Reverse Transcriptase Polymerase Chain Reaction, ZAP70, FOXP3, Cell Differentiation, Forkhead Transcription Factors, Flow Cytometry, Cell biology, Mice, Inbred C57BL, biology.protein, Lymph Nodes, Poly(ADP-ribose) Polymerases, Spleen
Abstract

Growing evidence is unveiling a role for poly(ADP-ribose) polymerase (PARP)-1 in the regulation of inflammatory/immune responses. In the current study, we investigated the effects of PARP-1 deficiency on regulatory T cell differentiation. Increased numbers of regulatory CD4+CD25+/Foxp3+ T cells were found in thymus, spleen, and lymph nodes of PARP-1 knockout (KO) mice compared with wild-type (WT) controls. The increased frequency of regulatory T cells in the periphery resulted in impaired CD4 cell proliferation and IL-2 production, which could be restored by CD25+ cell depletion. Phenotype and inhibitory functions of PARP-1 KO regulatory T cells were similar to WT cells, indicating that PARP-1 affects regulatory T cell differentiation rather than function. Purified naive CD4 cells from PARP-1 KO mice stimulated in vitro expressed forkhead box p3 mRNA at higher levels and generated a greater number of Foxp3+ cells (inducible regulatory T [iTreg] cells) than the WT counterpart. This finding was due to a higher rate of naive CD4 cell to Foxp3+ iTreg cell conversion rather than to higher resistance to apoptosis induction. Interestingly, PARP-1 deficiency did not affect retinoid-related orphan receptor-γt mRNA expression and differentiation of purified naive CD4 cells to Th17 cells. PARP-1 KO iTreg cells showed features similar to WT regulatory T cells, suggesting that modulation of PARP-1 during the immune response might be used to induce greater numbers of functional regulatory T cells. In conclusion, our findings represent the first evidence that PARP-1 can affect regulatory T cell differentiation and open new perspectives on potential targets for modulating immune responses.

Published
2010

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