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IntroductionResearch suggests that interprofessional education, bringing learners together to learn about, with, and from each other, improves health professions education and can improve health outcomes. Little research has measured outcomes of interprofessional education between midwifery students and obstetrics and gynecology residents. The purpose of this study was to examine self-assessed interprofessional and collaborative competencies among midwifery students and obstetrics and gynecology residents.MethodsBaseline self-assessed interprofessional and collaborative competencies were compared with follow-up measurements to evaluate learners' experiences over an 11-month study period. Participants were midwifery students and obstetrics and gynecology residents who experienced interprofessional learning activities. The Interprofessional Education Collaborative Competency Self-Assessment Survey (IPEC Survey) and Interprofessional Collaborative Competency Attainment Survey (ICCAS) were used.ResultsOf 256 learners at 4 demonstration sites, 223 (87%) completed the baseline, and 121 of 237 eligible learners (51%) completed the follow-up surveys. The IPEC Survey total score (t = 2.31, P = .02) and interaction subscale (t = 2.85, P = .005) and ICCAS score (t = 4.04, P = .001) increased for midwifery students but not obstetrics and gynecology residents on the IPEC Survey (t = 0.32, P = .75) and ICCAS (t = -0.05, P = .96) measures. Midwifery students (87%) and residents (57%) reported improved overall ability to collaborate. Learners responding to 3 open-ended questions valued team-based experiences, including learning how to communicate with each other; appreciated learning each other's education and scope of practice; and recommended skills development including uncommon clinical events, case discussions, and direct clinical care.DiscussionThis study advanced knowledge about interprofessional education between midwifery students and obstetrics and gynecology residents. Midwifery students improved in self-assessed interprofessional and collaborative competencies. Most learners reported better interprofessional collaboration skills and were positive about future interprofessional learning. This evaluation approach is available for other programs implementing or extending interprofessional education.

Introduction: Nilogen Oncosystems’ 3D-EXpress platform allows for the detailed characterization offreshly resected patient tumor tissue for inclusion in studies investigating rational combinations of IOtherapeutics. Incorporating data on immune cell composition, tumor cell target expression, mutationalstatus, and HPV infection within tumoroids generated from HNSCC tissues provides tremendous input toidentify candidate tissues for therapeutic testing. The 3D-EXpress platform employed here providesdetailed observations investigating tumor cell viability using confocal microscopy and immune cellactivation indicated by increases in cytokine release. Materials and Methods: 3D tumoroids measuring 150 microns in size were generated from fresh patientHNSCC resection samples collected with proper patient consent and relevant IRB approval. Tumoroidswere never enzymatically dissociated, propagated, or reassembled to maintain the intact tumormicroenvironment (TME). The resulting tumoroids were cryopreserved in the Biorepository, where eachsample had associated patient demographic data, tumor grade, stage, mutational and HPV status inaddition to a detailed tumor immune profile and a FFPE representative sample in TMA format for targetselection. Pooled tumoroids for each patient tumor were treated ex vivo both singly with a selectiveWEE1 inhibitor, AZD1775 and an ATR blocker AZD6738 alone and in combination with a PD-1 immunecheckpoint inhibitor antibody nivolumab for 72h. Results and Summary: To quantify treatment-mediated tumor cell killing we used 3D high-contentconfocal imaging and a proprietary algorithm for data analysis. Additionally, culture supernatantsisolated from treated tumoroids were used for multiplex cytokine detection to monitor changes in theTME upon ex vivo treatment. Responses to treatments were further correlated with the composition ofinnate and adaptive TIL populations within each tumor as assessed by 21-color flow cytometry inaddition to tumor mutational status, HPV infection, smoking status, as well as EGFR and PD-L1expression levels analyzed by multiplex IF on associated TMA slides. Conclusion: These results demonstrate that targeting DNA damage response and immune checkpointblockade may provide a potential combination strategy for the treatment of HNSCC. Furthermore, weshowed that our 3D-EXpress ex vivo tumoroid model provides a unique platform to rapidly assess theefficacy of drugs and drug combinations in fresh patient tumor samples with intact TME. Correlation ofex vivo drug responses with characteristics of each tumor’s immune microenvironment and detailedclinicopathological data may allow to identify clinically relevant biomarkers to enable the most effectivetreatment strategies for individual patients in the clinic. Citation Format: Jared Ehrhart, Seth Currlin, Sharon Camacho, Samantha Hoffman, Angie Rivera, Soner Altiok. 3D-EXpress ex vivo platform using a biorepository of characterized fresh patient tumoroids allows development of rational combinations with drugs targeting DNA damage response and immune checkpoint blockade. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 4572.

BACKGROUND Participant recruitment in rural and hard-to-reach/underserved populations can present unique challenges. These challenges are further exacerbated by the need for low-cost recruiting, which often leads to utilization of online recruitment methods (e.g., email, social media). Social media platforms are predominantly used by younger populations and require access to internet, which those with lower socioeconomic means and less education are less likely to have access to. The current study highlights how a recruitment strategy that utilizes email in combination with a follow-up phone call, produced high enrollment rates that included a diversity of participants from rural populations in older age brackets. OBJECTIVE To examine whether a combination of email and telephonic recruitment strategies increased recruitment rates in various hard-to-reach populations, specifically in rural and elderly populations METHODS We evaluated the overall enrollment rate of one recruitment arm of a larger study that aims to understand the relationship between genetics and substance use disorders. We evaluated the characteristics of the enrolled population to determine recruitment success of a combined email and follow-up telephonic recruitment strategy, as well as the rate of enrollment of hard-to-reach populations. These characteristics included, (1) enrollment rate before vs after follow-up phone call; (2) zip-code/county of enrollee to determine rural/urban and Appalachian status; (3) age to verify recruitment in all eligible age brackets; and (4) gender distribution among age brackets and rural/urban status. RESULTS The email and follow-up telephonic arm of the study had a 17.4% enrollment rate. Of those enrolled, 2297 (37.6%) of the participants enrolled before the follow-up phone call and 3809 (62.4%) enrolled after a follow-up phone call. A paired t-test with P= CONCLUSIONS Results indicate that combining an online recruitment method with a more traditional method, such as a phone call, yield higher enrollment rates than online methods alone. Adding a humanizing component, such as a live person phone call, may be a key element needed to establish trust and connection to encourage patients from underserved and rural areas to enroll in studies via virtual recruiting methods.

INTRODUCTION Transgender and nonbinary peoplehave been identified as vulnerable and clinically underserved and experience health disparities. Lack of health care provider knowledge about transgender and gender diverse populations is a barrier to care, impacting health outcomes. The aim of this project was to develop, implement, and evaluate a simulation learning activity for midwifery and women's health nurse practitioner students and obstetrics and gynecology residents to interact with transgender and nonbinary individuals, with the goal of enhancing health history taking comfort and skills. METHODS A partnership was formed among University faculty and simulation educators, a community clinic providing gender-affirming care, and members of transgender and gender diverse populations who served as patient-teachers. Two cases frequently encountered in clinics providing care to transgender individuals were developed for the simulation. Learners were divided into interprofessional groups of 3 and completed one of 2 case scenarios with a patient-teacher. Learners and patient-teachers debriefed after the simulation to discuss and reflect on the experience. RESULTS Thirty-three learners participated in the simulation, 12 midwifery students, 16 women's health nurse practitioner students and 5 obstetrics and gynecology residents. Significant differences were observed for all comfort and skills questions, showing increased perceived comfort and skills from pre- to postsimulation. There were no significant differences in attitude responses. DISCUSSION We successfully created and implemented a new sexual and reproductive history taking skills simulation in partnership with community clinic staff and transgender and nonbinary persons. The session was well received by learners and patient-teachers. Learners demonstrated significant improvements in comfort and skills in history taking in this setting and provided favorable feedback about the experience. This simulation can serve as a guide to others providing education to future midwives, women's health nurse practitioners, and obstetrician-gynecologists.

Class I methanol masers are found near regions of high-mass star formation and are pumped by collisions. Some indication of long-term variability in these masers has previously been reported (Kurtz et al. 2004). Here we will present evidence for variations in the intensity of the Class I methanol masers at 44 GHz on much shorter timescales. We present preliminary results of the observation of the 44 GHz Class I methanol transition toward sources in the DR21 region that indicates variability on timescales of days to possibly hours.

Early detection is the key to increased survival for women with ovarian cancer, yet a screening tool has yet to be developed that is adequately sensitive and specific enough for use in the general population. A need also exists for a reliable diagnostic method for women who present with an abdominal mass. In contrast, screening for cervical cancer by Pap tests has been routinely performed for over 50 years. In the liquid-based Pap test, cells are collected from the cervical os and placed into an alcohol-based fixative and then examined for abnormal cells. Since ovarian cancer cells have been observed in Pap tests, we reasoned that ovarian cancer peptide biomarkers may also be present. Our central hypothesis is that proteins shed by ovarian cancer cells can be detected in the Pap test fixative by Mass Spectrometry (MS)-based proteomics. The Pap test fixative is ideal for biomarker discovery since it is derived from a site near the ovarian cancer (i.e. proteins may be secreted or shed from the tumor and flow through the fallopian tube into the uterus and out the cervical opening). Recently, the fimbria of the fallopian tube have been suggested to be the true precursor to ovarian cancer, strengthening our hypothesis that ovarian cancer proteins will be found in the lower genital tract, perhaps even at early stages. To demonstrate the feasibility of this approach, we previously examined the proteins present in residual Pap test fixative samples from women with normal cervical cytology by MS, and described 152 proteins in the “Normal Pap Proteome.” (Boylan et al., Clinical Proteomics, 2014). The objective of this study is to discover ovarian cancer biomarkers in Pap test fixative samples by MS-based proteomic techniques. We used isobaric tags to quantify the proteins in liquid Pap test samples from women with ovarian cancer compared to women with benign gynecological disease and healthy women. Proteins were concentrated from “Mock” Pap tests, and samples were trypsin digested utilizing the STrap technique to increase the likelihood that proteins would be recovered. We then performed Tandem Mass Tag™ (TMT) 10-plex labeling of Pap test fixative samples from post-menopausal women: 3 with ovarian cancer, 3 with benign gynecological conditions, 3 healthy, and a Reference Control consisting of pooled samples. The 10 labeled samples were combined and run on 2D LC-MS/MS, followed by bioinformatics integration to discover proteins in Pap test fixatives that are unique and/or differentially expressed in the samples. We identified >3800 proteins total, and 22 proteins that were expressed at least 2-fold higher in all three ovarian cancer samples compared to the controls. We conclude that quantification of proteins from Pap test samples will prove a rich source of biomarkers for ovarian cancer detection. Citation Format: Amy P.N. Skubitz, Somi Afiuni, Kristin L.M. Boylan, Melissa Geller, Peter Argenta, Samantha Hoffman, Timothy Griffin. Tandem Mass Tag 10-plex isobaric labeling of Pap test proteins: A novel method for the identification of ovarian cancer protein biomarkers by mass spectrometry. [abstract]. In: Proceedings of the AACR Special Conference on Advances in Ovarian Cancer Research: Exploiting Vulnerabilities; Oct 17-20, 2015; Orlando, FL. Philadelphia (PA): AACR; Clin Cancer Res 2016;22(2 Suppl):Abstract nr B34.

We present results from neutral hydrogen (HI) observations of the tip of the Magellanic Stream (MS), obtained with the Arecibo telescope as a part of the on-going survey by the Consortium for Galactic studies with the Arecibo L-band Feed Array. We find four large-scale, coherent HI streams, extending continously over a length of 20 degrees, each stream possessing different morphology and velocity gradients. The newly discovered streams provide strong support for the tidal model of the MS formation by Connors et al. (2006), which suggested a spatial and kinematic bifurcation of the MS. The observed morphology and kinematics suggest that three of these streams could be interpreted as a 3-way splitting of the main MS filament, while the fourth stream appears much younger and may have originated from the Magellanic Bridge. We find an extensive population of HI clouds at the tip of the MS. Two thirds of clouds have an angular size in the range 3.5'--10'. We interpret this as being due to thermal instability, which would affect a warm tail of gas trailing through the Galactic halo over a characteristic timescale of a few Myrs to a few hundred Myrs. We show that thermal fragments can survive in the hot halo for a long time, especially if surrounded by a, Accepted by ApJ