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There is growing use of machine learning algorithms to replicate sub-grid parametrisation schemes in global climate models. Parametrisations rely on approximations, thus there is potential for machine learning to aid improvements. In this study, a neural network is used to mimic the behaviour of the non-orographic gravity wave scheme used in the Met Office climate model, important for stratospheric climate and variability. The neural network is found to require only two of the six inputs used by the parametrisation scheme, suggesting the potential for greater efficiency in this scheme. Use of a one-dimensional mechanistic model is advocated, allowing neural network hyperparameters to be trained based on emergent features of the coupled system with minimal computational cost, and providing a test bed prior to coupling to a climate model. A climate model simulation, using the neural network in place of the existing parametrisation scheme, is found to accurately generate a quasi-biennial oscillation of the tropical stratospheric winds, and correctly simulate the non-orographic gravity wave variability associated with the El Nino Southern Oscillation and stratospheric polar vortex variability. These internal sources of variability are essential for providing seasonal forecast skill, and the gravity wave forcing associated with them is reproduced without explicit training for these patterns.

Cys2His2 zinc finger (ZF) domains engineered to bind specific target sequences in the genome provide an effective strategy for programmable regulation of gene expression, with many potential therapeutic applications. However, the structurally intricate engagement of ZF domains with DNA has made their design challenging. Here we describe the screening of 49 billion protein–DNA interactions and the development of a deep-learning model, ZFDesign, that solves ZF design for any genomic target. ZFDesign is a modern machine learning method that models global and target-specific differences induced by a range of library environments and specifically takes into account compatibility of neighboring fingers using a novel hierarchical transformer architecture. We demonstrate the versatility of designed ZFs as nucleases as well as activators and repressors by seamless reprogramming of human transcription factors. These factors could be used to upregulate an allele of haploinsufficiency, downregulate a gain-of-function mutation or test the consequence of regulation of a single gene as opposed to the many genes that a transcription factor would normally influence.

The application of gamification for educational purposes has been widely reported with mixed results. Successful application, in most cases, is subjective and failures or mistakes are often downplayed when in reality, there is much to be learnt from these (failed) attempts. Despite the unique nature of each context and target audience, transferable principles that guide implementation are present and should be reported. This study applied a design-based research approach which offers a contextually sensitive, theoretically driven approach to the design and refinement of educational interventions. Through iterative implementations and qualitative data collection, over a 2-year period, we report on the process and outcome of gamifying the learning activities in an Industrial Psychology module to facilitate student engagement. The primary contribution of the study are proposed design principles for practitioners and researchers based on the lessons learned in this process. These highlight key considerations for applying gamification to support student engagement.

The purpose of this meta-methods study (i.e., involving the full[er] integration of multiple methods research approaches and mixed methods research approaches) was to examine challenges experienced by students at Stellenbosch University that hinder their ability successfully to learn online during the emergency remote teaching that began in April 2020 due to the COVID-19 pandemic. Specifically, 1,932 students completed an online questionnaire. Analyses of the open-ended responses via WordStat 8.0.29 topic modeling (i.e., crossover mixed analysis) led to the identification of seven (challenge) themes that categorised the students’ challenges. In turn, via a principal component analysis of the themes (i.e., crossover mixed analysis), these seven themes were collapsed into four dimensions (i.e., meta-themes). A latent class analysis (i.e., qualitizing) yielded seven profiles of students. Disaggregating the themes by demographic and location variables led to the identification of subgroups who are most at risk for experiencing each challenge type. Implications of these and other findings are discussed.

After the phase-out of polybrominated diphenyl ethers, their replacement compounds, organophosphate flame retardants (OPFRs) became ubiquitous in home and work environments. OPFRs, which may act as endocrine disruptors, are detectable in human urine, breast milk, and blood samples collected from pregnant women. However, the effects of perinatal OPFR exposure on offspring homeostasis and gene expression remain largely underexplored. To address this knowledge gap, virgin female mice were mated and dosed with either a sesame oil vehicle or an OPFR mixture (tris(1,3-dichloro-2-propyl)phosphate, tricresyl phosphate, and triphenyl phosphate, 1 mg/kg each) from gestational day (GD) 7 to postnatal day (PND) 14. Hypothalamic and hepatic tissues were collected from one female and one male pup per litter on PND 0 and PND 14. Expression of genes involved in energy homeostasis, reproduction, glucose metabolism, and xenobiotic metabolism were analyzed using quantitative real-time PCR. In the mediobasal hypothalamus, OPFR increased Pdyn, Tac2, Esr1, and Pparg in PND 14 females. In the liver, OPFR increased Pparg and suppressed Insr, G6pc, and Fasn in PND 14 males and increased Esr1, Foxo1, Dgat2, Fasn, and Cyb2b10 in PND 14 females. We also observed striking sex differences in gene expression that were dependent on the age of the pup. Collectively, these data suggest that maternal OPFR exposure alters hypothalamic and hepatic development by influencing neonatal gene expression in a sex-dependent manner. The long-lasting consequences of these changes in expression may disrupt puberty, hormone sensitivity, and metabolism of glucose, fatty acids, and triglycerides in the maturing juvenile.

The research object refers to the Concatenating a gridded rainfall reanalysis dataset into a time series notebook published in the Environmental Data Science book.

The research object refers to the Met Office UKV high-resolution atmosphere model data notebook published in the Environmental Data Science book.

The research object refers to the Met Office UKV high-resolution atmosphere model data notebook published in the Environmental Data Science book.

Orientation: The quality of basic education in South Africa is in need of interventions to improve the general standard of education offered in many public schools. Teachers and their work experiences are important factors that impact this standard.Research purpose: The aim of this study was to shed light on the factors that contribute to the experiences and outcomes of illegitimate tasks, as experienced by teachers, and the potential buffers to the negative effects of these tasks.Motivation for the study: The motivation for this study was to inform human resource practices and interventions to enhance the work experiences of teachers.Research approach/design and method: Exploratory qualitative research was conducted with 10 primary school teachers (n = 10) from a selected Western Cape education district. Responses to semi-structured individual interviews were transcribed verbatim, unedited and categorised into main themes through directed content analysis.Main findings: Environmental and psychological factors that lead to the experience of unnecessary and unreasonable illegitimate tasks, the time-consuming nature and outcomes of these tasks, as well as mechanisms that can buffer the harmful effects of illegitimate tasks, were identified.Practical/managerial implications: The identification of various contributing factors resulting in teachers’ experience of different types of illegitimate tasks and associated outcomes. Potential interventions and recommendations for future research are made.Contribution/value-addition: Qualitative studies regarding teachers’ experience of illegitimate tasks in the South African context are lacking. This article sheds light on the contributing factors, unnecessary and unreasonable tasks experienced and outcomes, as well as mechanisms that buffer the effect of illegitimate tasks amongst primary school teachers.

Abstract. Laws promoting equal employment opportunity (EEO) in favor of applicants from “protected groups” are commonplace in many countries. The present study compared graduate applicants from protected vs. unprotected groups. Drawing on the theory of planned behavior and on social cognitive theory we explored how intentions to apply for jobs are shaped by behavioral beliefs (internal factors) and protection status (external factor). Results from structural invariance analysis showed that, overall, beliefs influenced applicant intentions similarly in both groups. However, relative weights analysis revealed notable differences in the relative influence of attitude and norm beliefs. The findings suggest that recruitment practitioners and scholars may better understand how applicant intentions are formed if the role of protection status is considered in conjunction with applicant beliefs.

Organisation performance measurement is relevant for non-profit charitable organisations as they strive for security in an increasingly competitive funding environment. This study aimed to identify the priority measures and indicators of organisational performance of an Australian non-government charitable organisation that delivers non-acute health services. Seventy-seven and 59 participants across nine stakeholder groups responded to a two-staged Delphi technique study of a case study organisation. The stage one questionnaire was developed using information garnered through a detailed review of literature. Data from the first round were aggregated and analysed for the stage two survey. The final data represented a group consensus. Quality of care was ranked the most important of six organisational performance measures. Service user satisfaction was ranked second followed by financial performance, internal processes, employee learning and growth and community engagement. Thirteen priority indicators were determined across the six measures. Consensus was reached on the priority organisational performance measures and indicators. Stakeholders of the case study organisation value evidence-based practice, technical strength of services and service user satisfaction over more commercially orientated indicators.

To develop a set of recommendations for authors of qualitative studies in the field of health informatics, we conducted an extensive literature search and also manually checked major journals in the field of biomedical informatics and qualitative research looking for papers, checklists, and guidelines pertaining to assessing and reporting of qualitative studies. We synthesized the found criteria to develop an initial set of reporting recommendations that are particularly relevant to qualitative studies of health information technology systems. This paper presents a preliminary version of these recommendations. We are planning to refine and revise this version using comments and suggestions of experts in evaluation of health informatics applications and publish a detailed set of recommendations.

In 2008, EURIM, a UK-based group that ‘brings together politicians, officials and industry to . . . support of the creation of a globally competitive, socially inclusive and democratically accountable information society’ (EURIM, 2009) released a report entitled, ‘How to Achieve Citizen-Centric Service Delivery: Let the People Speak’.

Background: The Infectious Disease Clinic of Worcester Hospital introduced an integrated tuberculosis/human immunodeficiency virus (HIV) service in July 2009 to provide comprehensive management to patients who were co-infected with tuberculosis and HIV. Method: In a retrospective cohort study that was carried out from 1 July 2009 to 31 March 2010, the tuberculosis outcomes of co-infected patients attending the Infectious Disease Clinic for antiretroviral (ARV) treatment and receiving their tuberculosis medication at the Infectious Disease Clinic, were compared with those of patients receiving ARV treatment at the Infectious Disease Clinic and tuberculosis treatment at their local clinic. Results: Seventy-four per cent of patients completed their treatment and 26% were cured, with no defaults or deaths, in the tubercuolosis/HIV integrated cohort. Thirty-eight per cent completed their treatment, 45% were cured, 9% died and another 9% defaulted in the cohort receiving their tuberculosis treatment at a local clinic. This indicates that there was a significantly better tuberculosis outcome in the tuberculosis/HIV cohort (p-value < 0.05). C onclusion : The significantly better tuberculosis outcome that resulted when tuberculosis and HIV services were integrated led to services being integrated in the Breede Valley subdistrict. Keywords: tuberculosis/HIV treatment integration, human immunodeficiency virus, tuberculosis, outcomes

The quality of online medical information available for patients has long been a concern of health care professionals. [1-4] Although initiatives exist for patients to use when searching for information, there is the concern that these initiatives are either ineffective or even counter-productive. [5-6] Another criticism is that initiatives do not sufficiently achieve their respective goals. [7-8] It is important to consider that many initiatives have been designed with patients in mind, but not with patients involved. Various portals, seals, rating systems, ethical codes, etc., exist ostensibly for patients, but arose largely without concrete input from real patients-as-end-users. Literature addressing usability on the basis of studies assessing real patients' searching behaviors appeared only in 2002. [9-11] The conclusions from these studies, while insightful, reflect the need for ongoing research into the daily practices of patients searching for web-based health care information. This poster highlights the first results of a systematic ethnographic study (interviews and shadow-searching carried out between November, 2001 and August, 2003) to determine how patients approach health care information when searching on the internet and illustrates the different strategies that patients use to assess the health information they encounter on the web.