6 results on '"Sam Proesmans"'
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2. Dengue and chikungunya among outpatients with acute undifferentiated fever in Kinshasa, Democratic Republic of Congo: A cross-sectional study.
- Author
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Sam Proesmans, Freddy Katshongo, John Milambu, Blaise Fungula, Hypolite Muhindo Mavoko, Steve Ahuka-Mundeke, Raquel Inocêncio da Luz, Marjan Van Esbroeck, Kevin K Ariën, Lieselotte Cnops, Birgit De Smet, Pascal Lutumba, Jean-Pierre Van Geertruyden, and Veerle Vanlerberghe
- Subjects
Arctic medicine. Tropical medicine ,RC955-962 ,Public aspects of medicine ,RA1-1270 - Abstract
BACKGROUND:Pathogens causing acute fever, with the exception of malaria, remain largely unidentified in sub-Saharan Africa, given the local unavailability of diagnostic tests and the broad differential diagnosis. METHODOLOGY:We conducted a cross-sectional study including outpatient acute undifferentiated fever in both children and adults, between November 2015 and June 2016 in Kinshasa, Democratic Republic of Congo. Serological and molecular diagnostic tests for selected arboviral infections were performed on blood, including PCR, NS1-RDT, ELISA and IFA for acute, and ELISA and IFA for past infections. RESULTS:Investigation among 342 patients, aged 2 to 68 years (mean age of 21 years), with acute undifferentiated fever (having no clear focus of infection) revealed 19 (8.1%) acute dengue-caused by DENV-1 and/or DENV-2 -and 2 (0.9%) acute chikungunya infections. Furthermore, 30.2% and 26.4% of participants had been infected in the past with dengue and chikungunya, respectively. We found no evidence of acute Zika nor yellow fever virus infections. 45.3% of patients tested positive on malaria Rapid Diagnostic Test, 87.7% received antimalarial treatment and 64.3% received antibacterial treatment. DISCUSSION:Chikungunya outbreaks have been reported in the study area in the past, so the high seroprevalence is not surprising. However, scarce evidence exists on dengue transmission in Kinshasa and based on our data, circulation is more important than previously reported. Furthermore, our study shows that the prescription of antibiotics, both antibacterial and antimalarial drugs, is rampant. Studies like this one, elucidating the causes of acute fever, may lead to a more considerate and rigorous use of antibiotics. This will not only stem the ever-increasing problem of antimicrobial resistance, but will-ultimately and hopefully-improve the clinical care of outpatients in low-resource settings. TRIAL REGISTRATION:ClinicalTrials.gov NCT02656862.
- Published
- 2019
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3. Data mining methods for classification of Medium-Chain Acyl-CoA dehydrogenase deficiency (MCADD) using non-derivatized tandem MS neonatal screening data.
- Author
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Tim Van den Bulcke, Paul Vanden Broucke, Viviane Van Hoof, Kristien Wouters, Seppe vanden Broucke, Geert Smits, Elke Smits, Sam Proesmans, Toon Van Genechten, and François Eyskens
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- 2011
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4. Dengue and Chikungunya among Febrile Outpatients in Kinshasa, Democratic Republic of Congo: a cross-sectional study
- Author
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J geertruyden Van, Pascal Lutumba, R da Luz Inocencio, Kevin K. Ariën, B Smet De, Veerle Vanlerberghe, Blaise Fungula, John Milambu, Freddy Katshongo, Sam Proesmans, M Esbroeck Van, H Mavoko Muhindo, Steve Ahuka-Mundeke, and Lieselotte Cnops
- Subjects
03 medical and health sciences ,0302 clinical medicine ,Geography ,Cross-sectional study ,Environmental health ,parasitic diseases ,030231 tropical medicine ,medicine ,030212 general & internal medicine ,Chikungunya ,medicine.disease ,medicine.disease_cause ,Dengue fever - Abstract
Background Pathogens causing acute fever, with the exception of malaria, remain largely unidentified in sub-Saharan Africa, given the local unavailability of diagnostic tests and the broad differential diagnosis. Methodology/Principal Findings We conducted a cross-sectional study including outpatient acute febrile syndromes in both children and adults, between November 2015 and June 2016 in Kinshasa, Democratic Republic of Congo. Serological and molecular diagnostic tests for arboviral infections were performed on blood, including PCR and NS1-RDT for acute, and ELISA and IFAT for past infections. Conclusions/Significance Among 342 patients, aged 2 to 68 years, 45.3% tested positive on malaria Rapid Diagnostic Test. However, 87.7% received antimalarial and 64.3% antibacterial treatment. Further investigation among 235 fever cases revealed 19 (8.1%) acute dengue and 2 (0.9%) acute chikungunya infections, with an important proportion of participants already exposed to flaviviridae (possibly dengue) and alphaviridae (possibly chikungunya) in the past, namely 30.2 % and 26.4% respectively. We found no evidence of exposure to Zika nor yellow fever virus.
- Published
- 2018
5. Dengue and chikungunya among outpatients with acute undifferentiated fever in Kinshasa, Democratic Republic of Congo: A cross-sectional study
- Author
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Jean-Pierre Van Geertruyden, Marjan Van Esbroeck, Steve Ahuka-Mundeke, Sam Proesmans, Hypolite Muhindo Mavoko, Veerle Vanlerberghe, Freddy Katshongo, Raquel Inocêncio da Luz, Kevin K. Ariën, Birgit De Smet, Blaise Fungula, Pascal Lutumba, Lieselotte Cnops, and John Milambu
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Male ,RNA viruses ,0301 basic medicine ,Viral Diseases ,Physiology ,RC955-962 ,Fevers ,Dengue virus ,Pathology and Laboratory Medicine ,medicine.disease_cause ,Biochemistry ,Dengue fever ,Serology ,Dengue ,0302 clinical medicine ,Arctic medicine. Tropical medicine ,Immune Physiology ,Outpatients ,Medicine and Health Sciences ,Chikungunya ,Enzyme-Linked Immunoassays ,Child ,Rapid diagnostic test ,Chikungunya Virus ,Immune System Proteins ,Yellow fever ,Middle Aged ,Infectious Diseases ,Arboviral Infections ,Medical Microbiology ,Child, Preschool ,Viral Pathogens ,Viruses ,Democratic Republic of the Congo ,Female ,Public aspects of medicine ,RA1-1270 ,Pathogens ,Research Article ,Neglected Tropical Diseases ,Adult ,medicine.medical_specialty ,Adolescent ,Fever ,Alphaviruses ,Immunology ,030231 tropical medicine ,Research and Analysis Methods ,Microbiology ,Antibodies ,Togaviruses ,Young Adult ,03 medical and health sciences ,Signs and Symptoms ,Diagnostic Medicine ,Internal medicine ,parasitic diseases ,Parasitic Diseases ,medicine ,Humans ,Seroprevalence ,Immunoassays ,Microbial Pathogens ,Aged ,Biology and life sciences ,Flaviviruses ,business.industry ,Organisms ,Public Health, Environmental and Occupational Health ,Chikungunya Infection ,Proteins ,Dengue Virus ,Tropical Diseases ,medicine.disease ,Malaria ,Cross-Sectional Studies ,030104 developmental biology ,Immunologic Techniques ,Chikungunya Fever ,Human medicine ,business - Abstract
Background Pathogens causing acute fever, with the exception of malaria, remain largely unidentified in sub-Saharan Africa, given the local unavailability of diagnostic tests and the broad differential diagnosis. Methodology We conducted a cross-sectional study including outpatient acute undifferentiated fever in both children and adults, between November 2015 and June 2016 in Kinshasa, Democratic Republic of Congo. Serological and molecular diagnostic tests for selected arboviral infections were performed on blood, including PCR, NS1-RDT, ELISA and IFA for acute, and ELISA and IFA for past infections. Results Investigation among 342 patients, aged 2 to 68 years (mean age of 21 years), with acute undifferentiated fever (having no clear focus of infection) revealed 19 (8.1%) acute dengue–caused by DENV-1 and/or DENV-2 –and 2 (0.9%) acute chikungunya infections. Furthermore, 30.2% and 26.4% of participants had been infected in the past with dengue and chikungunya, respectively. We found no evidence of acute Zika nor yellow fever virus infections. 45.3% of patients tested positive on malaria Rapid Diagnostic Test, 87.7% received antimalarial treatment and 64.3% received antibacterial treatment. Discussion Chikungunya outbreaks have been reported in the study area in the past, so the high seroprevalence is not surprising. However, scarce evidence exists on dengue transmission in Kinshasa and based on our data, circulation is more important than previously reported. Furthermore, our study shows that the prescription of antibiotics, both antibacterial and antimalarial drugs, is rampant. Studies like this one, elucidating the causes of acute fever, may lead to a more considerate and rigorous use of antibiotics. This will not only stem the ever-increasing problem of antimicrobial resistance, but will–ultimately and hopefully–improve the clinical care of outpatients in low-resource settings. Trial registration ClinicalTrials.gov NCT02656862., Author summary Malaria remains one of the most important causes of fever in sub-Saharan Africa. However, its share is declining, since the diagnosis and treatment of malaria have improved significantly over the years. Hence leading to an increase in the number of patients presenting with non-malarial fever. Often, obvious clinical signs and symptoms like cough or diarrhea are absent, probing the question: “What causes the fever?” Previous studies have shown that the burden of arboviral infections–like dengue and chikungunya–in sub-Saharan Africa is underestimated, which is why we screened for four common arboviral infections in patients presenting with ‘undifferentiated fever’ at an outpatient clinic in suburban Kinshasa, Democratic Republic of Congo. Among the patients tested, we found that one in ten presented with an acute arboviral infection and that almost one in three patients had been infected in the past. These findings suggest that clinicians should think about arboviral infections more often, thereby refraining from the prescription of antibiotics, a practice increasingly problematic given the global rise of antimicrobial resistance.
- Published
- 2019
6. Data mining methods for classification of Medium-Chain Acyl-CoA dehydrogenase deficiency (MCADD) using non-derivatized tandem MS neonatal screening data
- Author
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Toon Van Genechten, François Eyskens, Paul Vanden Broucke, Geert Smits, Sam Proesmans, Viviane Van Hoof, Seppe vanden Broucke, Kristien Wouters, Elke Smits, and Tim Van den Bulcke
- Subjects
blood spots ,newborns ,Computer science ,diagnosis ,Population ,MCADD ,Logistic regression ,Feature selection ,Health Informatics ,computer.software_genre ,Acyl-CoA Dehydrogenase ,Lipid Metabolism, Inborn Errors ,Neonatal Screening ,Text mining ,Belgium ,Artificial Intelligence ,Tandem Mass Spectrometry ,medicine ,Humans ,education ,Medium-Chain Acyl-CoA dehydrogenase ,Data mining ,education.field_of_study ,Newborn screening ,business.industry ,logistic regression ,medium-chain acyl-coa dehydrogenase ,Infant, Newborn ,rare diseases ,acid oxidation disorders ,data mining ,mass-spectrometry ,medicine.disease ,Computer Science Applications ,Rare diseases ,Test set ,Hyperparameter optimization ,Human medicine ,business ,computer ,mcadd - Abstract
Newborn screening programs for severe metabolic disorders using tandem mass spectrometry are widely used. Medium-Chain Acyl-CoA dehydrogenase deficiency (MCADD) is the most prevalent mitochondrial fatty acid oxidation defect (1:15,000 newborns) and it has been proven that early detection of this metabolic disease decreases mortality and improves the outcome. In previous studies, data mining methods on derivatized tandem MS datasets have shown high classification accuracies. However, no machine learning methods currently have been applied to datasets based on non-derivatized screening methods. A dataset with 44,159 blood samples was collected using a non-derivatized screening method as part of a systematic newborn screening by the PCMA screening center (Belgium). Twelve MCADD cases were present in this partially MCADD-enriched dataset. We extended three data mining methods, namely C4.5 decision trees, logistic regression and ridge logistic regression, with a parameter and threshold optimization method and evaluated their applicability as a diagnostic support tool. Within a stratified cross-validation setting, a grid search was performed for each model for a wide range of model parameters, included variables and classification thresholds. The best performing model used ridge logistic regression and achieved a sensitivity of 100%, a specificity of 99.987% and a positive predictive value of 32% (recalibrated for a real population), obtained in a stratified cross-validation setting. These results were further validated on an independent test set. Using a method that combines ridge logistic regression with variable selection and threshold optimization, a significantly improved performance was achieved compared to the current state-of-the-art for derivatized data, while retaining more interpretability and requiring less variables. The results indicate the potential value of data mining methods as a diagnostic support tool.
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