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Considerable change is occurring in the structure and operation of electric power systems throughout the world. This paper describes some of these changes, the forces creating them and the possible reliability issues associated with them.

In this paper, the most frequently used maintenance strategies are reviewed. Distinction is made between strategies where maintenance consists of replacement by a new (or 'good as new') component and where it is represented by a less costly activity resulting in a limited improvement of the component's condition. Methods are also divided into categories where maintenance is performed at fixed intervals and where it is carried out as needed. A further distinction is made between heuristic methods and those based on mathematical models; the models themselves can be deterministic or probabilistic. From a review of present maintenance policies in electric utilities it is concluded that maintenance at fixed intervals is the most frequently used approach, often augmented by additional corrections. Newer 'as needed'-type methods, such as reliability-centered maintenance (RCM), are increasingly considered for application in North America, but methods based on mathematical models are hardly ever used or even considered. Yet only mathematical approaches where component deterioration and condition improvement by maintenance are quantitatively linked can determine the effect of maintenance on reliability. Although more complex, probabilistic models have advantages over deterministic ones: they are capable of describing actual processes more realistically, and also facilitate optimization for maximal reliability or minimal costs. Index Terms--Maintenance, overhaul, power system reliability, probability: applications, reliability modeling.

In the liberalized energy market and under the framework of the European Union Renewables Directive, Italy's commitment to wind power is very ambitious. This article focuses on the evolution of wind power in Italy from the perspective of a major player in the supply industry. The regulatory aspects are also covered. [ABSTRACT FROM PUBLISHER]

In 2016 the Italian National Dna database for forensic purposes finally went live. After seven years of "operational life", numbers and results are still scarce. The aim of the study is to evaluate the actual knowledge and perceived usefulness of this investigative tool among a significant sample of Italian magistrates. The research was conducted through the administration of an anonymous structured questionnaire to magistrates belonging to the Court of Milano. The overall picture emerging from the questionnaire is a worrying lack of preparation regarding the functioning of the Dna database among judges and public prosecutors. This situation affects the performance of the instrument. The authors believe that a specific and compulsory training for magistrates should be introduced., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

In 1997, it was discovered that maternal plasma contains Cell-Free Fetal DNA (cffDNA). cffDNA has been investigated as a source of DNA for non-invasive prenatal testing for fetal pathologies, as well as for non-invasive paternity testing. While the advent of Next Generation Sequencing (NGS) led to the routine use of Non-Invasive Prenatal Screening (NIPT or NIPS), few data are available regarding the reliability and reproducibility of Non-Invasive Prenatal Paternity Testing (NIPPT or NIPAT). Here, we present a non-invasive prenatal paternity test (NIPAT) analyzing 861 Single Nucleotide Variants (SNV) from cffDNA through NGS technology. The test, validated on more than 900 meiosis samples, generated log(CPI)(Combined Paternity Index) values for designated fathers ranging from +34 to +85, whereas log(CPI) values calculated for unrelated individuals were below -150. This study suggests that NIPAT can be used with high accuracy in real cases.

The role of DNA damage in PCR processivity/fidelity is a relevant topic in molecular investigation of aged/forensic samples. In order to reproduce one of the most common lesions occurring in postmortem tissues, a new protocol based on aqueous hydrolysis of the DNA was developed in vitro. Twenty-five forensic laboratories were then provided with 3.0 μg of a trial sample (TS) exhibiting, in mean, the loss of 1 base of 20, and a molecular weight below 300 bp. Each participating laboratory could freely choose any combination of methods, leading to the quantification and to the definition of the STR profile of the TS, through the documentation of each step of the analytical approaches selected. The results of the TS quantification by qPCR showed significant differences in the amount of DNA recorded by the participating laboratories using different commercial kits. These data show that only DNA quantification "relative" to the used kit (probe) is possible, being the "absolute" amount of DNA inversely related to the length of the target region (r(2) = 0.891). In addition, our results indicate that the absence of a shared stable and certified reference quantitative standard is also likely involved. STR profiling was carried out selecting five different commercial kits and amplifying the TS for a total number of 212 multiplex PCRs, thus representing an interesting overview of the different analytical protocols used by the participating laboratories. Nine laboratories decided to characterize the TS using a single kit, with a number of amplifications varying from 2 to 12, obtaining only partial STR profiles. Most of the participants determined partial or full profiles using a combination of two or more kits, and a number of amplifications varying from 2 to 27. The performance of each laboratory was described in terms of number of correctly characterized loci, dropped-out markers, unreliable genotypes, and incorrect results. The incidence of unreliable and incorrect genotypes was found to be higher for participants carrying out a limited number of amplifications, insufficient to define the correct genotypes from damaged DNA samples such as the TS. Finally, from a dataset containing about 4500 amplicons, the frequency of PCR artifacts (allele dropout, allele drop-in, and allelic imbalance) was calculated for each kit showing that the new chemistry of the kits is not able to overcome the concern of template-related factors. The results of this collaborative exercise emphasize the advantages of using a standardized degraded DNA sample in the definition of which analytical parameters are critical for the outcome of the STR profiles., (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)