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3. The Junctional Adhesion Molecule C (JAMC) Determines the Invasive Properties of Glioblastoma Stem-Like Cells at the Endothelial Interface

Author

Rosinska, Sara, primary, Andre-Gregoire, Gwennan, additional, Kerherve, Mathilde, additional, Trillet, Kilian, additional, Brigliadori Fugio, Lais, additional, Roux, Quentin, additional, Le Gall, Morgane, additional, Salnot, Virginie, additional, Faurobert, Eva, additional, Merlet, Laura, additional, Marionneau-Lambot, Severine, additional, Cherel, Michel, additional, Nedellec, Steven, additional, Barbier, Emmanuel L., additional, Collomb, Nora, additional, Rome, Claire, additional, Bidere, Nicolas, additional, and Gavard, Julie, additional

Published
2024
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4. G-CSF reshapes the cytosolic PCNA scaffold and modulates glycolysis in neutrophils

Author

Aymonnier, Karen, primary, Bosetta, Enzo, additional, Leborgne, Nathan G F, additional, Ullmer, Audrey, additional, Le Gall, Morgane, additional, De Chiara, Alessia, additional, Salnot, Virginie, additional, Mani, Souganya, additional, Scapini, Patrizia, additional, Wicks, Ian, additional, Chatfield, Simon, additional, Martin, Katherine R, additional, and Witko-Sarsat, Veronique, additional

Published
2023
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5. G-CSF reshapes the cytosolic PCNA scaffold and modulates glycolysis in neutrophils.

Author

Aymonnier, Karen, Bosetta, Enzo, Leborgne, Nathan G F, Ullmer, Audrey, Gall, Morgane Le, Chiara, Alessia De, Salnot, Virginie, Many, Souganya, Scapini, Patrizia, Wicks, Ian, Chatfield, Simon, Martin, Katherine R, and Witko-Sarsat, Véronique

Subjects
PROLIFERATING cell nuclear antigen, HOMEOSTASIS, GRANULOCYTE-colony stimulating factor, NEUTROPHILS, GLYCOLYSIS
Abstract

Cytosolic proliferating cell nuclear antigen (PCNA) is involved in neutrophil survival and function, in which it acts as a scaffold and associates with proteins involved in apoptosis, NADPH oxidase activation, cytoskeletal dynamics, and metabolism. While the PCNA interactome has been characterized in neutrophils under homeostatic conditions, less is known about neutrophil PCNA in pathophysiological contexts. Granulocyte colony-stimulating factor (G-CSF) is a cytokine produced in response to inflammatory stimuli that regulates many aspects of neutrophil biology. Here, we used isolated normal-density neutrophils from G-CSF–treated haemopoietic stem cell donors (GDs) as a model to understand the role of PCNA during inflammation. Proteomic analysis of the neutrophil cytosol revealed significant differences between GDs and healthy donors (HDs). PCNA was one of the most upregulated proteins in GDs, and the PCNA interactome was significantly different in GDs compared with HDs. Importantly, while PCNA associated with almost all enzymes involved in glycolysis in HDs, these associations were decreased in GDs. Functionally, neutrophils from GDs had a significant increase in glycolysis compared with HDs. Using p21 competitor peptides, we showed that PCNA negatively regulates neutrophil glycolysis in HDs but had no effect on GD neutrophils. These data demonstrate that G-CSF alters the PCNA scaffold, affecting interactions with key glycolytic enzymes, and thus regulates glycolysis, the main energy pathway utilized by neutrophils. By this selective control of glycolysis, PCNA can organize neutrophils functionality in parallel with other PCNA mechanisms of prolonged survival. PCNA may therefore be instrumental in the reprogramming that neutrophils undergo in inflammatory or tumoral settings. [ABSTRACT FROM AUTHOR]

Published
2024
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7. Red blood cell proteomics reveal remnant protein biosynthesis and folding pathways in PIEZO1-related hereditary xerocytosis

Author

Caulier, Alexis, primary, Jankovsky, Nicolas, additional, Gautier, Emilie Fleur, additional, El Nemer, Wassim, additional, Guitton, Corinne, additional, Ouled-Haddou, Hakim, additional, Guillonneau, François, additional, Mayeux, Patrick, additional, Salnot, Virginie, additional, Bruce, Johanna, additional, Picard, Véronique, additional, and Garçon, Loïc, additional

Published
2022
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8. Multiparametric characterization of red blood cell physiology after hypotonic dialysis based drug encapsulation process

Author

Robert, Mélanie, primary, Laperrousaz, Bastien, additional, Piedrahita, Diana, additional, Gautier, Emilie-Fleur, additional, Nemkov, Travis, additional, Dupuy, Florian, additional, Nader, Elie, additional, Salnot, Virginie, additional, Mayeux, Patrick, additional, D'Alessandro, Angelo, additional, Lavazec, Catherine, additional, Joly, Philippe, additional, Scheer, Alexander, additional, Connes, Philippe, additional, and Cibiel, Agnès, additional

Published
2022
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9. Caractérisation de globules rouges après encapsulation de molécules thérapeutiques

Author

Robert, Mélanie, primary, Laperrousaz, Bastien, additional, Piedrahita, Diana, additional, Gautier, Emilie-Fleur, additional, Nemkov, Travis, additional, Dupuy, Florian, additional, Nader, Elie, additional, Salnot, Virginie, additional, Mayeux, Patrick, additional, D’Alessandro, Angelo, additional, Lavazec, Catherine, additional, Joly, Philippe, additional, Connes, Philippe, additional, and Cibiel, Agnes, additional

Published
2021
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10. Impact of Fetal Growth Restriction on the Neonatal Microglial Proteome in the Rat

Author

Zinni, Manuela, primary, Pansiot, Julien, additional, Colella, Marina, additional, Faivre, Valérie, additional, Delahaye-Duriez, Andrée, additional, Guillonneau, François, additional, Bruce, Johanna, additional, Salnot, Virginie, additional, Mairesse, Jérôme, additional, Knoop, Marit, additional, Possovre, Marie-Laure, additional, Vaiman, Daniel, additional, and Baud, Olivier, additional

Published
2021
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11. Comprehensive Analysis of Transcript and Protein Relative Abundance During Blood Stages of Plasmodium falciparum Infection

Author

Kamaliddin, Claire, primary, Guillochon, Emilie, additional, Salnot, Virginie, additional, Rombaut, David, additional, Huguet, Stéphanie, additional, Guillonneau, François, additional, Houzé, Sandrine, additional, Cot, Michel, additional, Deloron, Philippe, additional, Argy, Nicolas, additional, and Bertin, Gwladys I., additional

Published
2021
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12. Proteome analysis of formalin‐fixed paraffin‐embedded colorectal adenomas reveals the heterogeneous nature of traditional serrated adenomas compared to other colorectal adenomas

Author

Sohier, Pierre, Sanson, Romain, Leduc, Marjorie, Audebourg, Anne, Broussard, Cedric, Salnot, Virginie, Just, Pierre-Alexandre, Pasmant, Eric, Mayeux, Patrick, Guillonneau, Francois, Romagnolo, Béatrice, Perret, Christine, Terris, Benoit, Service de Pathologie [AP-HP Hôpital Cochin], Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Sorbonne Paris Cité (USPC), Plateforme protéomique 3P5 [Institut Cochin] (3P5), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service de biochimie et de génétique moléculaire [CHU Cochin], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), and Core fundings came from INSERM grants, and grants from and from the Cancéropôle Îlede-France. The Orbitrap Fusion mass spectrometer was acquired with funds from the FEDER through the 'Operational Programme for Competitiveness and Employment 2007-2013' and from the Cancéropôle Île-de-France.

Subjects
Proteomics, stomatognathic diseases, [SDV]Life Sciences [q-bio], Label free quantification, Colon adenoma, [SDV.CAN]Life Sciences [q-bio]/Cancer, LC-MS/MS, Sessile serrated adenoma, formalin-fixed paraffin-embedded, FFPE, Traditional serrated adenoma
Abstract

International audience; Traditional serrated adenoma (TSA) remains the least understood of all the colorectal adenomas although these lesions have been associated with a significant cancer risk, twice that of the conventional adenoma (CAD) and of the sessile serrated adenoma (SSA/P). This study was performed to investigate the proteomic profiles of the different colorectal adenomas to better understand the pathogenesis of TSA. We performed a global quantitative proteome analysis using the label-free quantification (LFQ) method on 44 colorectal adenomas (12 TSAs, 15 CADs, 17 SSA/Ps) and 17 normal colonic mucosa samples, archived as formalin-fixed paraffin-embedded blocks. Unsupervised consensus hierarchical clustering applied to the whole proteomic profile of the 44 colorectal adenomas identified four subtypes: C1 and C2 were well-individualized clusters composed of all the CADs (15/15) and most of the SSA/Ps (13/17), respectively. This is consistent with the fact that CADs and SSA/Ps are homogeneous and distinct colorectal adenoma entities. In contrast, TSAs were subdivided into C3 and C4 clusters, consistent with the more heterogeneous entity of TSA at the morphological and molecular levels. Comparison of the proteome expression profile between the adenoma subtypes and normal colonic mucosa further confirmed the heterogeneous nature of TSAs which overlapped either on CADs or SSA/Ps, while CADs and SSAs formed homogeneous and distinct entities. Furthermore, we identified LEFTY1 a new potential marker for TSAs that may be relevant for the pathogenesis of TSA. LEFTY1 is an inhibitor of the Nodal/TGFβ pathway, which we found to be one of the most overexpressed proteins specifically in TSAs. This finding was confirmed by immunohistochemistry. Our study confirms that CADs and SSA/Ps form homogeneous and distinct colorectal adenoma entities, while TSAs are a heterogeneous entity and may arise from either SSA/Ps or from normal mucosa evolving through a process related to the conventional adenoma pathway. This article is protected by copyright. All rights reserved.

Published
2019

13. Lack of the multidrug transporter MRP4/ABCC4 defines the PEL-negative blood group and impairs platelet aggregation

Author

Azouzi, Slim, primary, Mikdar, Mahmoud, additional, Hermand, Patricia, additional, Gautier, Emilie-Fleur, additional, Salnot, Virginie, additional, Willemetz, Alexandra, additional, Nicolas, Gaël, additional, Vrignaud, Cédric, additional, Raneri, Alexandre, additional, Mayeux, Patrick, additional, Bole-Feysot, Christine, additional, Nitschké, Patrick, additional, Cartron, Jean-Pierre, additional, Colin, Yves, additional, Hermine, Olivier, additional, Jedlitschky, Gabriele, additional, Cloutier, Marc, additional, Constanzo-Yanez, Jessica, additional, Ethier, Carole, additional, Robitaille, Nancy, additional, St-Louis, Maryse, additional, Le Van Kim, Caroline, additional, and Peyrard, Thierry, additional

Published
2020
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14. The Multidrug Transporter MRP4/ABCC4 Involved in the Leukemia Clinical Course Specifies the Novel PEL Human Blood Group System

Author

Azouzi, Slim, primary, Mikdar, Mahmoud, primary, Hermand-Tournamille, Patricia, primary, Gautier, Emilie-Fleur, primary, Salnot, Virginie, primary, Willemetz, Alexandra, primary, Nicolas, Gaël, primary, Vrignaud, Cedric, primary, Raneri, Alexandre, primary, Mayeux, Patrick, primary, Bole-Feysot, Christine, primary, Nitschke, Patrick, primary, Cartron, Jean-Pierre, primary, Colin Aronovicz, Yves, primary, Hermine, Olivier, primary, Jedlitschky, Gabriele, primary, Cloutier, Marc, primary, Constanzo-Yanez, Jessica, primary, Ethier, Carole, primary, Robitaille, Nancy, primary, St-Louis, Maryse, primary, Le Van Kim, Caroline, primary, and Peyrard, Thierry, primary

Published
2019
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16. PFI1785w : a highly conserved protein associated with pregnancy associated malaria

Author

Kamaliddin, Claire, Salnot, Virginie, Leduc, Marjorie, Ezinmegnon, Sem, Broussard, Cédric, Fievet, Nadine, Deloron, Philippe, Guillonneau, François, and Bertin, Gwladys I.

Subjects
Proteomics, Plasmodium, lcsh:Medicine, Protein Structure Prediction, Biochemistry, Mass Spectrometry, Protein Structure, Secondary, Database and Informatics Methods, Pregnancy, Macromolecular Structure Analysis, Medicine and Health Sciences, Malaria, Falciparum, lcsh:Science, Protozoans, Malarial Parasites, Eukaryota, Genomics, Phylogeography, embryonic structures, Female, Synthetic Biology, Sequence Analysis, Research Article, Protein Structure, Bioinformatics, Plasmodium falciparum, Antigens, Protozoan, Research and Analysis Methods, Parasite Groups, Malaria Vaccines, parasitic diseases, Genetics, Parasitic Diseases, Humans, Molecular Biology, lcsh:R, Organisms, Biology and Life Sciences, Proteins, Tropical Diseases, Parasitic Protozoans, Malaria, Pregnancy Complications, Parasitic, Mutation, lcsh:Q, Parasitology, Structural Genomics, Peptides, Sequence Alignment, Apicomplexa
Abstract

Pregnancy-associated malaria (PAM) is one of the severe forms of Plasmodium falciparum infection. The main antigen VAR2CSA is the target of vaccine development. However, the large size of VAR2CSA protein and its high degree of variability limit to the efficiency of the vaccination. Using quantitative mass spectrometry method, we detected and quantified proteotypic peptides from 5 predicted PAM associated proteins. Our results confirmed that PFI1785w is over-expressed in PAM samples. Then, we investigated PFI1785w variability among a set of parasite samples from various endemic areas. PFI1785w appear to be more conserved than VAR2CSA. PFB0115w, another PAM associated protein, seems also associated with the pathology. Further vaccination strategies could integrate other proteins in addition to the major VAR2CSA antigen to improve immune response to vaccination.

Published
2017

17. Proteome analysis of formalin‐fixed paraffin‐embedded colorectal adenomas reveals the heterogeneous nature of traditional serrated adenomas compared to other colorectal adenomas.

Author

Sohier, Pierre, Sanson, Romain, Leduc, Marjorie, Audebourg, Anne, Broussard, Cédric, Salnot, Virginie, Just, Pierre‐Alexandre, Pasmant, Eric, Mayeux, Patrick, Guillonneau, François, Romagnolo, Béatrice, Perret, Christine, and Terris, Benoît

Subjects
PROTEOMICS, PATHOLOGY, MUCOUS membranes
Abstract

Traditional serrated adenoma (TSA) remains the least understood of all the colorectal adenomas, although these lesions have been associated with a significant cancer risk, twice that of the conventional adenoma (CAD) and of the sessile serrated adenoma (SSA/P). This study was performed to investigate the proteomic profiles of the different colorectal adenomas to better understand the pathogenesis of TSA. We performed a global quantitative proteome analysis using the label‐free quantification (LFQ) method on 44 colorectal adenoma (12 TSAs, 15 CADs, and 17 SSA/Ps) and 17 normal colonic mucosa samples, archived as formalin‐fixed paraffin‐embedded blocks. Unsupervised consensus hierarchical clustering applied to the whole proteomic profile of the 44 colorectal adenomas identified four subtypes: C1 and C2 were well‐individualized clusters composed of all the CADs (15/15) and most of the SSA/Ps (13/17), respectively. This is consistent with the fact that CADs and SSA/Ps are homogeneous and distinct colorectal adenoma entities. In contrast, TSAs were subdivided into C3 and C4 clusters, consistent with the more heterogeneous entity of TSA at the morphologic and molecular levels. Comparison of the proteome expression profile between the adenoma subtypes and normal colonic mucosa further confirmed the heterogeneous nature of TSAs, which overlapped either on CADs or SSA/Ps, whereas CADs and SSAs formed homogeneous and distinct entities. Furthermore, we identified LEFTY1 a new potential marker for TSAs that may be relevant for the pathogenesis of TSA. LEFTY1 is an inhibitor of the Nodal/TGFβ pathway, which we found to be one of the most overexpressed proteins specifically in TSAs. This finding was confirmed by immunohistochemistry. Our study confirms that CADs and SSA/Ps form homogeneous and distinct colorectal adenoma entities, whereas TSAs are a heterogeneous entity and may arise from either SSA/Ps or from normal mucosa evolving through a process related to the CAD pathway. © 2019 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Published
2020
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18. Deep proteomic analysis of chicken erythropoiesis

Author

Leduc, Marjorie, primary, Gautier, Emilie-Fleur, additional, Guillemin, Anissa, additional, Broussard, Cédric, additional, Salnot, Virginie, additional, Lacombe, Catherine, additional, Gandrillon, Olivier, additional, Guillonneau, François, additional, and Mayeux, Patrick, additional

Published
2018
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20. Comprehensive Proteomic Analysis of Human Erythropoiesis

Author

Gautier, Emilie-Fleur, primary, Ducamp, Sarah, additional, Leduc, Marjorie, additional, Salnot, Virginie, additional, Guillonneau, François, additional, Dussiot, Michael, additional, Hale, John, additional, Giarratana, Marie-Catherine, additional, Raimbault, Anna, additional, Douay, Luc, additional, Lacombe, Catherine, additional, Mohandas, Narla, additional, Verdier, Frédérique, additional, Zermati, Yael, additional, and Mayeux, Patrick, additional

Published
2016
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22. Deep Proteomic Analysis of Human Erythropoiesis

Author

Ducamp, Sarah, primary, Gautier, Emilie-Fleur, additional, Leduc, Marjorie, additional, Salnot, Virginie, additional, Dussiot, Michael, additional, Guillonneau, François, additional, Giarratana, Marie-Catherine, additional, Douay, Luc, additional, Lacombe, Catherine, additional, Verdier, Frederique, additional, Zermati, Yael, additional, and Mayeux, Patrick, additional

Published
2015
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23. Mass spectrometry detection of G3m and IGHG3 alleles and follow-up of differential mother and neonate IgG3

Author

Dechavanne, Célia; Guillonneau, François; Chiappetta, Giovanni; Sago, Laïla; Lévy, Prisca; Salnot, Virginie; Guitard, Evelyne; Ehrenmann, François; Broussard, Cédric; Chafey, Philippe; Le Port, Agnès; Vinh, Joëlle; Mayeux, Patrick; Dugoujon, Jean-Michel; Lefranc, Marie-Paule; Migot-Nabias, Florence, http://orcid.org/0000-0002-8337-6569 Le Port, Agnes, Dechavanne, Célia; Guillonneau, François; Chiappetta, Giovanni; Sago, Laïla; Lévy, Prisca; Salnot, Virginie; Guitard, Evelyne; Ehrenmann, François; Broussard, Cédric; Chafey, Philippe; Le Port, Agnès; Vinh, Joëlle; Mayeux, Patrick; Dugoujon, Jean-Michel; Lefranc, Marie-Paule; Migot-Nabias, Florence, and http://orcid.org/0000-0002-8337-6569 Le Port, Agnes

Abstract

PR, IFPRI3; ISI, PHND

Published
2012

24. Mass Spectrometry Detection of G3m and IGHG3 Alleles and Follow-Up of Differential Mother and Neonate IgG3

Author

Dechavanne, Célia, primary, Guillonneau, François, additional, Chiappetta, Giovanni, additional, Sago, Laïla, additional, Lévy, Prisca, additional, Salnot, Virginie, additional, Guitard, Evelyne, additional, Ehrenmann, François, additional, Broussard, Cédric, additional, Chafey, Philippe, additional, Le Port, Agnès, additional, Vinh, Joëlle, additional, Mayeux, Patrick, additional, Dugoujon, Jean-Michel, additional, Lefranc, Marie-Paule, additional, and Migot-Nabias, Florence, additional

Published
2012
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