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6. Comparison of Tests for Detection of ß-Lactamase-Producing Staphylococci

Author

Pitka¨la¨, A., Salmikivi, L., Bredbacka, P., Myllyniemi, A.-L., and Koskinen, M. T.

Abstract

ABSTRACTPenicillin resistance identification tests are important in veterinary medicine. Six enzyme assays and a PCR test were compared for the detection of ß-lactamase production or the ß-lactamase gene in 175 staphylococcal isolates. We conclude that the PCR test and two nitrocefin-based assays can be recommended for routine clinical use.

Published
2007
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7. Comparison of tests for detection of beta-lactamase-producing staphylococci.

Author

Pitkälä A, Salmikivi L, Bredbacka P, Myllyniemi AL, and Koskinen MT

Subjects
Animals, Cattle, Cattle Diseases microbiology, Coagulase metabolism, Colorimetry methods, Microbial Sensitivity Tests, Sensitivity and Specificity, Staphylococcal Infections microbiology, Staphylococcal Infections veterinary, Staphylococcus drug effects, Staphylococcus genetics, Staphylococcus isolation & purification, Staphylococcus aureus classification, Staphylococcus aureus enzymology, Staphylococcus aureus genetics, Staphylococcus aureus isolation & purification, beta-Lactamases genetics, Cephalosporins pharmacology, Indicators and Reagents pharmacology, Mastitis, Bovine microbiology, Polymerase Chain Reaction methods, Staphylococcus enzymology, beta-Lactamases biosynthesis
Abstract

Penicillin resistance identification tests are important in veterinary medicine. Six enzyme assays and a PCR test were compared for the detection of beta-lactamase production or the beta-lactamase gene in 175 staphylococcal isolates. We conclude that the PCR test and two nitrocefin-based assays can be recommended for routine clinical use.

Published
2007
Full Text
View/download PDF

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