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4. Fungal Lipases: Versatile Tools for White Biotechnology

Author

Pérez, Malena Martínez, Gonçalves, Enrico Cerioni Spiropulos, Vici, Ana Claudia, Salgado, Jose Carlos Santos, de Moraes Polizeli, Maria de Lourdes Teixeira, Gupta, Vijai Kumar, Series Editor, Tuohy, Maria G., Series Editor, Yadav, Ajar Nath, editor, Mishra, Shashank, editor, Singh, Sangram, editor, and Gupta, Arti, editor

Published
2019
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7. A novel Trichoderma reeseimutant RP698 with enhanced cellulase production

Author

Silva, Jean Carlos Rodrigues, Salgado, José Carlos Santos, Vici, Ana Claudia, Ward, Richard John, Polizeli, Maria Lourdes Teixeira Moraes, Guimarães, Luis Henrique Souza, Furriel, Rosa Prazeres Melo, and Jorge, João Atílio

Abstract

A new strain of Trichoderma reesei(teleomorph Hypocrea jecorina) with high cellulase production was obtained by exposing the spores from T. reeseiQM9414 to an ultraviolet light followed by selecting fast-growing colonies on plates containing CMC (1% w/v) as the carbon source. The mutant T. reeseiRP698 reduced cultivation period to 5 days and increased tolerance to the end-products of enzymatic cellulose digestion. Under submerged fermentation conditions, FPase, CMCase, and Avicelase production increased up to 2-fold as compared to the original QM9414 strain. The highest levels of cellulase activity were obtained at 27 °C after 72 h with Avicel®, cellobiose, and sugarcane bagasse as carbon sources. The temperature and pH activity optima of the FPase, CMCase, and Avicelase were approximately 60 °C and 5.0, respectively. The cellulase activity was unaffected by the addition of 140 mM glucose in the enzyme assay. When T. reeseiRP698 crude extract was supplemented by the addition of β-glucosidase from Scytalidium thermophilum, a 2.3-fold increase in glucose release was observed, confirming the low inhibition by the end-product of cellulose hydrolysis. These features indicate the utility of this mutant strain in the production of enzymatic cocktails for biomass degradation.

Published
2024
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