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Fragile X syndrome (FXS) is caused by hypermethylation of the FMR1 promoter due to the full mutation expansion (full mutation [FM]: CGG ≥ 200 repeats) and silencing of FMR1. Assessment of mosaicism for active-unmethylated alleles has prognostic utility. This study examined relationships between FMR1 methylation in different tissues with FMR1 messenger ribonucleic acid (mRNA) and intellectual functioning in 87 males with FXS (1.89-43.17 years of age). Methylation sensitive Southern blot (mSB) and Methylation Specific-Quantitative Melt Aanalysis (MS-QMA) were used to examine FMR1 methylation. FMR1 mRNA levels in blood showed strong relationships with FMR1 methylation assessed using MS-QMA in blood (n = 68; R2 = 0.597; p = 1.4 × 10-10 ) and buccal epithelial cells (BEC) (n = 62; R2 = 0.24; p = 0.003), with these measures also showing relationships with intellectual functioning scores (p < 0.01). However, these relationships were not as strong for mSB, with ~40% of males with only FM alleles that were 100% methylated and non-mosaic by mSB, showing methylation mosaicism by MS-QMA. This was confirmed through presence of detectable levels of FMR1 mRNA in blood. In summary, FMR1 methylation levels in blood and BEC examined by MS-QMA were significantly associated with FMR1 mRNA levels and intellectual functioning in males with FXS. These relationships were not as strong for mSB, which underestimated prevalence of mosaicism.

In the present work, experimental results from the literature on the effect of CO on the NH oxidation in the absence and presence of NO are supplemented with novel flow reactor results and interpreted in terms of a detailed chemical kinetic model. The kinetic model provides a satisfactory prediction over a wide range of conditions for oxidation in flow reactors and for flame speeds of CO/NH. With increasing levels of CO, the generation of chain carriers gradually shifts from being controlled by the amine reaction subset to being dominated by the oxidation chemistry of CO, facilitating reaction at lower temperatures. At elevated temperature, presence of CO causes a change in selectivity of NH oxidation from N to NO. The present work provides a thorough evaluation of the amine subset of the reaction mechanism for the investigated conditions and offers a kinetic model that reliably can be used for post-flame oxidation modeling in engines and gas turbines fueled by ammonia with a hydrocarbon or alcohol as co-fuel.

An orchard sprayer prototype running a variable-rate algorithm to adapt the spray volume to the canopy characteristics (dimensions, shape and leaf density) in real-time was designed and implemented. The developed machine was able to modify the application rate by using an algorithm based on the tree row volume (TRV), in combination with a newly coefficient defined as Density Factor (Df). Variations in the canopy characteristics along the row crop were electronically measured using six ultrasonic sensors (three per sprayer side), This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 773718 (OPTimised Integrated pest Management for precise detection and control of plant diseases in perennial crops and open- field vegetables, www.optima-h2020.eu). Bernat Salas received funding for his Ph.D. development from Agència de Gestió D'Ajuts Universitaris I de Recerca (AGAUR) (file N° FI_B 01193), Preprint

New spraying systems embedded with different technologies have been developed for pesticide application in 3D crops. However, while applied to specific tree crops, the potential spray drift mitigation for advanced spraying systems needs to be classified due to the great variability of spray equipment and canopy structure. Here a precision spraying system was developed and compared with two typical spraying systems (conventional system, optimized system following the best management practices) for the applied volume/pesticide and spray drift in an apple orchard at two growth stages following the ISO22866-2005 protocol. Compared to the conventional system, the other two advanced systems significantly reduced the amount of ground drift (>60%) at most of the sampling distances at the growth stage BBCH 72, while the precision system demonstrated the best drift mitigation (57.3% reduction) at the stage BBCH 99. For the airborne drift, a remarkable drift reduction was also achieved with the two advanced systems. Specifically, the optimized spraying system exhibited a drift reduction by approximately 80% at the first growth stage, and the precision application demonstrated its considerable advantages in minimizing drift loss for the sparse canopy at the stage BBCH 99. Moreover, the saving of applied volume/pesticide was achieved by 12% with the optimized system and 43% with the precision system. This study revealed the necessity and prospect of the advanced spraying systems to reduce the environmental contamination and health risk from pesticide applications in fruit tree production., This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 773718 (OPTimised Integrated pest Management for precise detection and control of plant diseases in perennial crops and open-field vegetables, www.optima-h2020.eu). Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature., Peer Reviewed, Postprint (published version)

Air assistance and droplet’s characteristics influence on the pesticide deposit and its distribution over the intended target during the spray application process. The present research work is focused on the characterization of the leaf coverage and the overall spray distribution within the whole canopy by combining different settings of air assistance with different nozzles, generating different droplet’s sizes. A new designed air-assisted orchard sprayer was tested, presenting a wireless remote-regulation system to control the airflow rate from the fan by adjusting the blade pitch. In this way, five airflow rates were obtained by combining the gearbox position and the blade pitch. For each one of these five air settings, three droplet sizes (F-Fine, M-Medium, and C-Coarse) were combined and evaluated over a set of artificial apple trees., This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 773718 (OPTimised Integrated pest Management for precise detection and control of plant diseases in perennial crops and open-field vegetables, www.optima-h2020.eu). Bernat Salas received funding for his Ph.D. development from Agència de Gestió D'Ajuts Universitaris I de Recerca (AGAUR) (file N° FI_B 01193), Postprint (updated version)