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2. Dynamics of viral DNA shedding and culture viral DNA positivity in different clinical samples collected during the 2022 mpox outbreak in Lombardy, Italy

Author

Cutrera, Miriam, Cuomo, Marianna, De Poli, Federica, Campanini, Giulia, Pitrolo, Antonino Maria Guglielmo, Iskandar, Elizabeth, Cassaniti, Irene, Bruno, Raffaele, Rizzardini, Giuliano, Puoti, Massimo, Castelli, Francesco, Corsico, Laura, Giacomelli, Andrea, Pozza, Giacomo, Casalini, Giacomo, Raccagni, Angelo, Trentacapilli, Bendetta, Bertoni, Costanza, Bruzzesi, Elena, Candela, Caterina, Tesoro, Daniele, Mule, Giovanni, Bandera, Alessandra, Bianca Mariani, Antonio Muscatello, Maffeo, Manuel, Vecchio, Riccardo, Piccinelli, Sara, Piralla, Antonio, Mileto, Davide, Rizzo, Alberto, Ferrari, Guglielmo, Giardina, Federica, Gaiarsa, Stefano, Petazzoni, Greta, Bianchi, Micol, Salari, Federica, Bracchitta, Fiorenza, Sammartino, Josè Camilla, Ferrari, Alessandro, Gagliardi, Gloria, Mancon, Alessandro, Fenizia, Claudio, Biasin, Mara, Rovida, Francesca, Paolucci, Stefania, Percivalle, Elena, Lombardi, Alessandra, Micheli, Valeria, Nozza, Silvia, Castagna, Antonella, Moschese, Davide, Antinori, Spinello, Gori, Andrea, Bonfanti, Paolo, Rossotti, Roberto, D'Arminio Monforte, Antonella, Attanasi, Federica, Tirani, Marcello, Cereda, Danilo, Baldanti, Fausto, and Gismondo, Maria Rita

Published
2024
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5. Evaluation of analytical performance of the STANDARD™ M10 MPX/OPX assay for the simultaneous DNA detection and clade attribution of Monkeypox virus.

Author

Mancon, Alessandro, Raccagni, Angelo Roberto, Gagliardi, Gloria, Moschese, Davide, Rizzo, Alberto, Giacomelli, Andrea, Cutrera, Miriam, Salari, Federica, Bracchitta, Fiorenza, Antinori, Spinello, Gori, Andrea, Rizzardini, Giuliano, Castagna, Antonella, Gismondo, Maria Rita, Nozza, Silvia, and Mileto, Davide

Published
2024
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6. Active Surveillance of Patients Colonized with CRE: A Single-Center Study Based on a Combined Molecular/Culture Protocol.

Author

Orena, Beatrice Silvia, Liporace, Maria Francesca, Teri, Antonio, Girelli, Daniela, Salari, Federica, Mutti, Michela, Giordano, Gabriele, Alteri, Claudia, Gentiloni Silverj, Flaminia, Matinato, Caterina, Callegaro, Annapaola, and Cariani, Lisa

Abstract

Background/Objectives: Carbapenem-resistant Enterobacteriaceae (CRE) are types of bacteria that need urgent attention globally. Active surveillance programs at hospitals are essential for the early identification of CRE carriers and the timely adoption of infection control measures. We aimed to analyze the epidemiology of CRE identified by multiplex RT-PCR in rectal swabs of patients upon admission to high-risk wards and to compare data obtained from both molecular and culture CRE screening. Methods: A total of 2861 rectal swabs, prospectively collected within 12–24 h of admission, underwent molecular screening for identification of K. pneumoniae carbapenemase (KPC), New Delhi metallo-β-lactamase (NDM), Verona integron-mediated metallo-β-lactamase (VIM), imipenemase (IMP), and OXA-48 (AllplexTM Entero-DR Assay). Only samples that tested positive or invalid underwent culture testing (Agar MacConkey and CHROMID® CARBA plates, bioMérieux, Craponne, France). Results: A total of 118 out of 2861 (about 4%) were positive for at least one carbapenem-resistant gene by a molecular approach (MA), with KPC, NDM, and VIM having the highest prevalence. Culture testing confirmed the presence of carbapenemase in 89 samples (75.4%), showing a disagreement rate of about 25% between the two methods, which, unfortunately, rises up to 60% for VIM. The dominant bacterial species were K. pneumoniae and E. coli (MALDI-TOF mass spectrometry). Conclusions: Our data underlined the need for the molecular screening of CRE carriers in order to implement active surveillance protocol in critical care settings and to improve infection control measures. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Surge of Mpox cases in Lombardy region, Italy, October 2023 - January 2024

Author

Moschese, D, Raccagni, A, Giacomelli, A, Piralla, A, Rossotti, R, Raimondi, A, Tesoro, D, Vezzosi, L, Gulletta, M, Clementi, N, Venturelli, S, Benardon, S, Ricaboni, D, Bernacchia, D, Mancon, A, Rovida, F, Attanasi, F, Cereda, D, Gismondo, M, Gori, A, Rizzardini, G, Antinori, S, Baldanti, F, Marzano, A, Marchetti, G, Castelli, F, Castagna, A, Nozza, S, Mileto, D, Bonfanti, P, Biasioli, L, Bruno, R, Vittoria Cossu, M, Fusetti, C, Lazzarin, S, Locatelli, M, Morosi, M, Piscaglia, M, Pusterla, L, Pozza, G, Ramoni, S, Rusconi, S, Rizzi, M, Rizzo, A, Sabaini, F, Salari, F, Sampaolo, M, Puoti, M, Tirani, M, Rachele Tomasoni, L, Moschese, Davide, Raccagni, Angelo Roberto, Giacomelli, Andrea, Piralla, Antonio, Rossotti, Roberto, Raimondi, Alessandro, Tesoro, Daniele, Vezzosi, Luigi, Gulletta, Maurizio, Clementi, Nicola, Venturelli, Serena, Benardon, Susanna, Ricaboni, Davide, Bernacchia, Dario, Mancon, Alessandro, Rovida, Francesca, Attanasi, Federica, Cereda, Danilo, Gismondo, Maria Rita, Gori, Andrea, Rizzardini, Giuliano, Antinori, Spinello, Baldanti, Fausto, Marzano, Angelo Valerio, Marchetti, Giulia, Castelli, Francesco, Castagna, Antonella, Nozza, Silvia, Mileto, Davide, Bonfanti, Paolo, Biasioli, Lorenzo, Bruno, Raffaele, Vittoria Cossu, Maria, Fusetti, Chiara, Lazzarin, Samuel, Locatelli, Massimo, Morosi, Manuela, Piscaglia, Marco, Pusterla, Luigi, Pozza, Giacomo, Ramoni, Stefano, Rusconi, Stefano, Rizzi, Marco, Rizzo, Alberto, Sabaini, Federico, Salari, Federica, Sampaolo, Michela, Puoti, Massimo, Tirani, Marcello, Rachele Tomasoni, Lina, Moschese, D, Raccagni, A, Giacomelli, A, Piralla, A, Rossotti, R, Raimondi, A, Tesoro, D, Vezzosi, L, Gulletta, M, Clementi, N, Venturelli, S, Benardon, S, Ricaboni, D, Bernacchia, D, Mancon, A, Rovida, F, Attanasi, F, Cereda, D, Gismondo, M, Gori, A, Rizzardini, G, Antinori, S, Baldanti, F, Marzano, A, Marchetti, G, Castelli, F, Castagna, A, Nozza, S, Mileto, D, Bonfanti, P, Biasioli, L, Bruno, R, Vittoria Cossu, M, Fusetti, C, Lazzarin, S, Locatelli, M, Morosi, M, Piscaglia, M, Pusterla, L, Pozza, G, Ramoni, S, Rusconi, S, Rizzi, M, Rizzo, A, Sabaini, F, Salari, F, Sampaolo, M, Puoti, M, Tirani, M, Rachele Tomasoni, L, Moschese, Davide, Raccagni, Angelo Roberto, Giacomelli, Andrea, Piralla, Antonio, Rossotti, Roberto, Raimondi, Alessandro, Tesoro, Daniele, Vezzosi, Luigi, Gulletta, Maurizio, Clementi, Nicola, Venturelli, Serena, Benardon, Susanna, Ricaboni, Davide, Bernacchia, Dario, Mancon, Alessandro, Rovida, Francesca, Attanasi, Federica, Cereda, Danilo, Gismondo, Maria Rita, Gori, Andrea, Rizzardini, Giuliano, Antinori, Spinello, Baldanti, Fausto, Marzano, Angelo Valerio, Marchetti, Giulia, Castelli, Francesco, Castagna, Antonella, Nozza, Silvia, Mileto, Davide, Bonfanti, Paolo, Biasioli, Lorenzo, Bruno, Raffaele, Vittoria Cossu, Maria, Fusetti, Chiara, Lazzarin, Samuel, Locatelli, Massimo, Morosi, Manuela, Piscaglia, Marco, Pusterla, Luigi, Pozza, Giacomo, Ramoni, Stefano, Rusconi, Stefano, Rizzi, Marco, Rizzo, Alberto, Sabaini, Federico, Salari, Federica, Sampaolo, Michela, Puoti, Massimo, Tirani, Marcello, and Rachele Tomasoni, Lina

Published
2024

12. Dynamics of viral DNA shedding and culture viral DNA positivity in different clinical samples collected during the 2022 mpox outbreak in Lombardy, Italy

Author

Piralla, A, Mileto, D, Rizzo, A, Ferrari, G, Giardina, F, Gaiarsa, S, Petazzoni, G, Bianchi, M, Salari, F, Bracchitta, F, Sammartino, J, Ferrari, A, Gagliardi, G, Mancon, A, Fenizia, C, Biasin, M, Rovida, F, Paolucci, S, Percivalle, E, Lombardi, A, Micheli, V, Nozza, S, Castagna, A, Moschese, D, Antinori, S, Gori, A, Bonfanti, P, Rossotti, R, Monforte, A, Attanasi, F, Tirani, M, Cereda, D, Baldanti, F, Gismondo, M, Cutrera, M, Cuomo, M, De Poli, F, Campanini, G, Pitrolo, A, Iskandar, E, Cassaniti, I, Bruno, R, Rizzardini, G, Puoti, M, Castelli, F, Corsico, L, Giacomelli, A, Pozza, G, Casalini, G, Raccagni, A, Trentacapilli, B, Bertoni, C, Bruzzesi, E, Candela, C, Tesoro, D, Mule, G, Bandera, A, Bianca Mariani, A, Maffeo, M, Vecchio, R, Piccinelli, S, Piralla, Antonio, Mileto, Davide, Rizzo, Alberto, Ferrari, Guglielmo, Giardina, Federica, Gaiarsa, Stefano, Petazzoni, Greta, Bianchi, Micol, Salari, Federica, Bracchitta, Fiorenza, Sammartino, Josè Camilla, Ferrari, Alessandro, Gagliardi, Gloria, Mancon, Alessandro, Fenizia, Claudio, Biasin, Mara, Rovida, Francesca, Paolucci, Stefania, Percivalle, Elena, Lombardi, Alessandra, Micheli, Valeria, Nozza, Silvia, Castagna, Antonella, Moschese, Davide, Antinori, Spinello, Gori, Andrea, Bonfanti, Paolo, Rossotti, Roberto, Monforte, Antonella D'arminio, Attanasi, Federica, Tirani, Marcello, Cereda, Danilo, Baldanti, Fausto, Gismondo, Maria Rita, Cutrera, Miriam, Cuomo, Marianna, De Poli, Federica, Campanini, Giulia, Pitrolo, Antonino Maria Guglielmo, Iskandar, Elizabeth, Cassaniti, Irene, Bruno, Raffaele, Rizzardini, Giuliano, Puoti, Massimo, Castelli, Francesco, Corsico, Laura, Giacomelli, Andrea, Pozza, Giacomo, Casalini, Giacomo, Raccagni, Angelo, Trentacapilli, Bendetta, Bertoni, Costanza, Bruzzesi, Elena, Candela, Caterina, Tesoro, Daniele, Mule, Giovanni, Bandera, Alessandra, Bianca Mariani, Antonio Muscatello, Maffeo, Manuel, Vecchio, Riccardo, Piccinelli, Sara, Piralla, A, Mileto, D, Rizzo, A, Ferrari, G, Giardina, F, Gaiarsa, S, Petazzoni, G, Bianchi, M, Salari, F, Bracchitta, F, Sammartino, J, Ferrari, A, Gagliardi, G, Mancon, A, Fenizia, C, Biasin, M, Rovida, F, Paolucci, S, Percivalle, E, Lombardi, A, Micheli, V, Nozza, S, Castagna, A, Moschese, D, Antinori, S, Gori, A, Bonfanti, P, Rossotti, R, Monforte, A, Attanasi, F, Tirani, M, Cereda, D, Baldanti, F, Gismondo, M, Cutrera, M, Cuomo, M, De Poli, F, Campanini, G, Pitrolo, A, Iskandar, E, Cassaniti, I, Bruno, R, Rizzardini, G, Puoti, M, Castelli, F, Corsico, L, Giacomelli, A, Pozza, G, Casalini, G, Raccagni, A, Trentacapilli, B, Bertoni, C, Bruzzesi, E, Candela, C, Tesoro, D, Mule, G, Bandera, A, Bianca Mariani, A, Maffeo, M, Vecchio, R, Piccinelli, S, Piralla, Antonio, Mileto, Davide, Rizzo, Alberto, Ferrari, Guglielmo, Giardina, Federica, Gaiarsa, Stefano, Petazzoni, Greta, Bianchi, Micol, Salari, Federica, Bracchitta, Fiorenza, Sammartino, Josè Camilla, Ferrari, Alessandro, Gagliardi, Gloria, Mancon, Alessandro, Fenizia, Claudio, Biasin, Mara, Rovida, Francesca, Paolucci, Stefania, Percivalle, Elena, Lombardi, Alessandra, Micheli, Valeria, Nozza, Silvia, Castagna, Antonella, Moschese, Davide, Antinori, Spinello, Gori, Andrea, Bonfanti, Paolo, Rossotti, Roberto, Monforte, Antonella D'arminio, Attanasi, Federica, Tirani, Marcello, Cereda, Danilo, Baldanti, Fausto, Gismondo, Maria Rita, Cutrera, Miriam, Cuomo, Marianna, De Poli, Federica, Campanini, Giulia, Pitrolo, Antonino Maria Guglielmo, Iskandar, Elizabeth, Cassaniti, Irene, Bruno, Raffaele, Rizzardini, Giuliano, Puoti, Massimo, Castelli, Francesco, Corsico, Laura, Giacomelli, Andrea, Pozza, Giacomo, Casalini, Giacomo, Raccagni, Angelo, Trentacapilli, Bendetta, Bertoni, Costanza, Bruzzesi, Elena, Candela, Caterina, Tesoro, Daniele, Mule, Giovanni, Bandera, Alessandra, Bianca Mariani, Antonio Muscatello, Maffeo, Manuel, Vecchio, Riccardo, and Piccinelli, Sara

Abstract

Background: Mpox virus (MPXV) has recently spread outside of sub-Saharan Africa. This large multicentre study was conducted in Lombardy, the most densely populated Italian region accounting for more than 40% of Italian cases. The present study aims to: i) evaluate the presence and the shedding duration of MPXV DNA in different body compartments correlating the MPXV viability with the time to onset of symptoms; ii) provide evidence of MPXV persistence in different body compartment as a source of infection and iii) characterize the MPXV evolution by whole genome sequencing (WGS) during the outbreak occurred in Italy. Material and methods: The study included 353 patients with a laboratory-confirmed diagnosis of MPXV infection screened in several clinical specimens in the period May 24th - September 1st, 2022. Viral isolation was attempted from different biological matrices and complete genome sequencing was performed for 61 MPXV strains. Results: MPXV DNA detection was more frequent in the skin (94.4%) with the longest median time of viral clearance (16 days). The actively-replicating virus in cell culture was obtained for 123/377 (32.6%) samples with a significant higher viral quantity on isolation positive samples (20 vs 31, p < 0.001). The phylogenetic analysis highlighted the high genetic identity of the MPXV strains collected, both globally and within the Lombardy region. Conclusion: Skin lesion is gold standard material and the high viral load and the actively-replicating virus observed in genital sites confirms that sexual contact plays a key role in the viral transmission.

Published
2024

13. Evaluation of 16S-Based Metagenomic NGS as Diagnostic Tool in Different Types of Culture-Negative Infections.

Author

Rimoldi, Sara Giordana, Tamoni, Alessandro, Rizzo, Alberto, Longobardi, Concetta, Pagani, Cristina, Salari, Federica, Matinato, Caterina, Vismara, Chiara, Gagliardi, Gloria, Cutrera, Miriam, and Gismondo, Maria Rita

Subjects
NUCLEOTIDE sequencing, CUTIBACTERIUM acnes, STAPHYLOCOCCUS epidermidis, BACTERIAL cultures, CARDIAC surgery
Abstract

Bacterial infections pose significant global health challenges, often underestimated due to difficulties in accurate diagnosis, especially when culture-based diagnostics fail. This study assesses the effectiveness of 16S-based metagenomic next generation sequencing (NGS) for identifying pathogens in culture-negative clinical samples across various medical settings. Overall, 48% of samples were collected from orthopedics, 15% from neurosurgery, and 12% in cardiac surgery, among others. The detection rate of monomicrobial infections was 68.6%, and 5.7% for polymicrobial infections. In addition, NGS detected bacteria in all samples from the lungs, head and neck, and eye specimens. Cutibacterium acnes (11%, 12/105) was the most frequent microorganism, followed by Staphylococcus epidermidis (10.4%, 11/105), and Staphylococcus aureus (9.5%, 10/105). In conclusion, 16S-targeted metagenomic sequencing enhances pathogen detection capabilities, particularly in instances where traditional cultures fail. By the combination of NGS and bacterial cultures, microbiologists might provide a more accurate diagnosis, guiding more effective treatments and potentially reducing healthcare costs associated with empirical treatments. [ABSTRACT FROM AUTHOR]

Published
2024
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14. Anal HPV prevalence in individuals with and without other concomitant sexually transmitted infections.

Author

Rizzo, Alberto, Moschese, Davide, Salari, Federica, Giacomelli, Andrea, Cavallo, Andrea, Cossu, Maria Vittoria, Morelli, Loriana, Fusetti, Chiara, Carrozzo, Giorgia, Reato, Serena, Micheli, Valeria, Antinori, Spinello, Lombardi, Alessandra, Gori, Andrea, and Gismondo, Maria Rita

Subjects
SEXUALLY transmitted diseases, TRICHOMONAS vaginalis, HUMAN papillomavirus, NEISSERIA gonorrhoeae, CHLAMYDIA trachomatis
Abstract

The association between human papillomavirus (HPV) and other sexually transmitted infections (STIs) in anal lesions still remains unclear. Aim of the study was to evaluate the prevalence of simultaneous infection of HPV and Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, and Trichomonas vaginalis in individuals screened for HPV anal infection. A total of 507 anal samples were tested for both anal HPV and STIs: 16% resulted positive for one or more non‐HPV STIs. Specifically, C. trachomatis, M. genitalium, and N. gonorrhoeae were detected in 8%, 5%, and 4% of cases, respectively. Two groups were considered, including a positive STI group and a negative STI group. The prevalence of HPV was similar in patients in both groups: high risk (HR)‐HPV and low risk (LR)‐HPV were 67% and 53% versus 62% (p = 0.361) and 54% (p = 0.864) of patients, respectively. However, HPV 16, 18, 35, 51, 59, and 69 were significantly more frequent in patients tested positive for other STIs versus HPV infection alone (p < 0.05). No significant differences between the two groups were observed in vaccination coverage, 28% versus 32% (p = 0.463), and HIV status, 86% versus 84% (p = 0.658). The study shows that the overall HPV status is not directly correlated to other STIs in the investigated population, except for certain HPV types, including HR‐HPV 16, reinforcing the urge for a greater vaccination coverage. [ABSTRACT FROM AUTHOR]

Published
2024
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17. Dynamics of viral DNA shedding and culture viral DNA positivity in different clinical samples collected during the 2022 mpox outbreak in Lombardy, Italy

Author

Piralla, Antonio, primary, Mileto, Davide, additional, Rizzo, Alberto, additional, Ferrari, Guglielmo, additional, Giardina, Federica, additional, Gaiarsa, Stefano, additional, Petazzoni, Greta, additional, Bianchi, Micol, additional, Salari, Federica, additional, Bracchitta, Fiorenza, additional, Sammartino, Josè Camilla, additional, Ferrari, Alessandro, additional, Gagliardi, Gloria, additional, Mancon, Alessandro, additional, Fenizia, Claudio, additional, Biasin, Mara, additional, Rovida, Francesca, additional, Paolucci, Stefania, additional, Percivalle, Elena, additional, Lombardi, Alessandra, additional, Micheli, Valeria, additional, Nozza, Silvia, additional, Castagna, Antonella, additional, Moschese, Davide, additional, Antinori, Spinello, additional, Gori, Andrea, additional, Bonfanti, Paolo, additional, Rossotti, Roberto, additional, Monforte, Antonella D'arminio, additional, Attanasi, Federica, additional, Tirani, Marcello, additional, Cereda, Danilo, additional, Baldanti, Fausto, additional, Gismondo, Maria Rita, additional, Cutrera, Miriam, additional, Cuomo, Marianna, additional, De Poli, Federica, additional, Campanini, Giulia, additional, Pitrolo, Antonino Maria Guglielmo, additional, Iskandar, Elizabeth, additional, Cassaniti, Irene, additional, Bruno, Raffaele, additional, Rizzardini, Giuliano, additional, Puoti, Massimo, additional, Castelli, Francesco, additional, Corsico, Laura, additional, Giacomelli, Andrea, additional, Pozza, Giacomo, additional, Casalini, Giacomo, additional, Raccagni, Angelo, additional, Trentacapilli, Bendetta, additional, Bertoni, Costanza, additional, Bruzzesi, Elena, additional, Candela, Caterina, additional, Tesoro, Daniele, additional, Mule, Giovanni, additional, Bandera, Alessandra, additional, Bianca Mariani, Antonio Muscatello, additional, Maffeo, Manuel, additional, Vecchio, Riccardo, additional, and Piccinelli, Sara, additional

Published
2024
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18. High prevalence of high‐risk HPV genotypes in individuals attending an infectious diseases clinic from 2018 to 2022 in Milan, Italy

Author

Rizzo, Alberto, primary, Moschese, Davide, additional, Salari, Federica, additional, Giacomelli, Andrea, additional, Morelli, Loriana, additional, Cossu, Maria Vittoria, additional, Fusetti, Chiara, additional, Petri, Francesco, additional, Casalini, Giacomo, additional, Poloni, Andrea, additional, Lazzarin, Samuel, additional, Gori, Andrea, additional, Antinori, Spinello, additional, Gismondo, Maria Rita, additional, Lombardi, Alessandra, additional, and Rizzardini, Giuliano, additional

Published
2024
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22. Detection and typization of HPV genotypes in subjects with oral and upper respiratory tract lesions, Milan, Italy.

Author

Rizzo, Alberto, Salari, Federica, Eplite, Angelo, Giacomelli, Andrea, Moschese, Davide, Dalu, Davide, Cossu, Maria Vittoria, Lorusso, Rosaria, Pozza, Giacomo, Morelli, Loriana, Fasola, Cinzia, Tonielli, Claudia, Fusetti, Chiara, De Cristofaro, Valeria, Gori, Andrea, Lombardi, Alessandra, Rizzardini, Giuliano, Gismondo, Maria Rita, and Antinori, Spinello

Subjects
*HUMAN papillomavirus, *GENITAL warts, *HEAD & neck cancer, *RESPIRATORY infections, *ORAL sex, *TONGUE cancer
Abstract

Oral human papillomavirus (HPV) is common among healthy individuals but causes and implications of persistent infections are under evaluation in the pathogenesis of head and neck neoplasms. This was a retrospective study evaluating the prevalence of high-risk (HR), probable HR and low-risk (LR) HPV types in patients reporting signs/symptoms of oral and upper respiratory tract lesions. Individuals attending between 2019 and 2022 a University Hospital in Milan, Italy, with risk factors for HPV (unprotected oral sex and/or previous documentation of HPV infection in oral and upper respiratory tract and/or another anatomical site) were included. Fourteen out of 110 (12.7%) individuals tested positive for HPV DNA. The prevalence of HR-HPV and LR-HPV was 3.6% (4/110) and 9.1% (10/110), respectively. No probable/possible HR-HPV was detected. Specifically, 10/110 (9.1%) were diagnosed with 1 LR-HPV genotype, 3/110 (2.7%) were infected with 1 HR-HPV and 1/110 had 3 concomitant HR-HPV types. HPV 16 (2.7%, 3/110) and 6 (4.5%, 5/110) were the most common HR and LR types, respectively. One patient positive for HPV 16, 33 and 35 was diagnosed with cancer at the base of the tongue. Two individuals among those who tested positive for HPV DNA reported previous HPV vaccination. Our data, in line with observations from previous prevalence studies, support the potential role of HPV in head and neck neoplasms. HPV DNA testing should be performed in patients presenting lesions in oral/respiratory tracts and risk factors for HPV. Improvement in HPV vaccination coverage is warranted. [ABSTRACT FROM AUTHOR]

Published
2024
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23. Traditional Cultures versus Next Generation Sequencing for Suspected Orthopedic Infection: Experience Gained from a Reference Centre

Author

Rimoldi, Sara Giordana, primary, Brioschi, Davide, additional, Curreli, Daniele, additional, Salari, Federica, additional, Pagani, Cristina, additional, Tamoni, Alessandro, additional, Longobardi, Concetta, additional, Bosari, Raffaella, additional, Rizzo, Alberto, additional, Landonio, Simona, additional, Coen, Massimo, additional, Passerini, Matteo, additional, Gismondo, Maria Rita, additional, Gori, Andrea, additional, and Manzotti, Alfonso, additional

Published
2023
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26. Neutralizing Antibody Titers Induced by JYNNEOS Vaccine in Unrecognized Previous Mpox Virus–Exposed Individuals

Author

Moschese, Davide, primary, Bianchi, Micol, additional, Cossu, Maria Vittoria, additional, Salari, Federica, additional, Giacomelli, Andrea, additional, Rizzo, Alberto, additional, Lazzarin, Samuel, additional, Sabaini, Federico, additional, Nozza, Silvia, additional, Mileto, Davide, additional, Gori, Andrea, additional, Antinori, Spinello, additional, Gismondo, Maria Rita, additional, and Rizzardini, Giuliano, additional

Published
2023
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27. Ongoing sporadic monkeypox cases: neutralising antibody detection in asymptomatic individuals

Author

Moschese, Davide, primary, Giacomelli, Andrea, additional, Mileto, Davide, additional, ANTINORI, SPINELLO, additional, BIANCHI, MICOL, additional, CAPETTI, AMEDEO FERIDNANDO, additional, CARUSO, FRANCESCO, additional, COLOMBO, MARTINA LAURA, additional, COSSU, MARIA VITTORIA, additional, CUTRERA, MIRIAM, additional, GISMONDO, MARIA RITA, additional, GORI, ANDREA, additional, LAZZARIN, SAMUEL, additional, LOMBARDI, ALESSANDRA, additional, MICHELI, VALERIA, additional, PASTENA, ANDREA, additional, POZZA, GIACOMO, additional, RIZZARDINI, GIULIANO, additional, RIZZO, ALBERTO, additional, SABAINI, FEDERICO, additional, and SALARI, FEDERICA, additional

Published
2023
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28. Viral bloodstream detection in mpox patients: An observational multicentric study

Author

Rizzo, Alberto, primary, Moschese, Davide, additional, Trentacapilli, Benedetta, additional, Cossu, Maria Vittoria, additional, Giacomelli, Andrea, additional, Salari, Federica, additional, Bianchi, Micol, additional, Raccagni, Angelo Roberto, additional, Micheli, Valeria, additional, Gori, Andrea, additional, Messina, Emanuela, additional, Antinori, Spinello, additional, Gismondo, Maria Rita, additional, Castagna, Antonella, additional, Mileto, Davide, additional, Rizzardini, Giuliano, additional, Lombardi, Alessandra, additional, and Nozza, Silvia, additional

Published
2023
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30. Gastrointestinal Parasite Infections and Environmental Sustainability of the Ovine Sector: Eimeria spp. Infections and Nitrogen and Phosphorus Excretions in Dairy Sheep in Italy.

Author

Sodi, Irene, Martini, Mina, Salari, Federica, and Perrucci, Stefania

Subjects
NITROGEN excretion, SUSTAINABILITY, EIMERIA, SUSTAINABLE agriculture, SHEEP ranches, SHEEP farming
Abstract

In sheep farming, gastrointestinal parasites can be responsible for significant reductions in animal health and production. Nitrogen (N) and phosphorus (P) fecal excretions are the main determining factors for N2O emissions from manure management and may pose other environmental problems, such as the acidification and eutrophication of natural habitats. By using the Mini-FLOTAC technique on fecal samples from sheep of different ages and physiological status from 19 dairy sheep farms in Tuscany (central Italy), gastrointestinal parasite infections were evaluated. The animal N and P fecal contents were also assessed, with the aim of evaluating possible relationships between the identified parasites and the environmental sustainability of the examined farms. The obtained results showed that Eimeria spp. (86.36%) and gastrointestinal strongyle (54.55%) infections are prevalent in the examined farms. Moreover, significantly higher (p ≤ 0.05) P and Eimeria oocyst/gram-of-feces (OPG) values were found in fecal samples from animals < 1 year of age, and a significant (p ≤ 0.05) positive correlation resulted between N content and Eimeria OPG in fecal samples from animals in the first month of lactation. The findings from this study suggest for the first time that Eimeria spp. infections may have an impact on the environmental sustainability of sheep farming. [ABSTRACT FROM AUTHOR]

Published
2023
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31. Disinfectant caps in vitro effectiveness.

Author

Rimoldi, Sara Giordana, La Cava, Leyla, Palladino, Chiara, Piacenza, Mariagrazia, Vimercati, Stefania, Cristina, Pagani, Salari, Federica, Curreli, Daniele, Gismondo, Maria Rita, Foschi, Antonella, Giustivi, Davide, Diotto, Veronica, Bizzi, Emanuele, Calloni, Maria, Casella, Francesco, Martini, Elena, Donadoni, Mattia, Cogliati, Chiara, and Gidaro, Antonio

Published
2023
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32. Role of multi-site sampling in the diagnosis of human Monkeypox

Author

Rizzo, Alberto, primary, Mileto, Davide, additional, Moschese, Davide, additional, Candela, Caterina, additional, Mancon, Alessandro, additional, Giacomelli, Andrea, additional, Raccagni, Angelo Roberto, additional, Salari, Federica, additional, Cossu, Maria Vittoria, additional, Micheli, Valeria, additional, Castagna, Antonella, additional, Rizzardini, Giuliano, additional, Lombardi, Alessandra, additional, Nozza, Silvia, additional, and Gismondo, Maria Rita, additional

Published
2023
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33. Persistent detection and sequencing of SARS‐CoV‐2 in the bloodstream of an immunocompromised patient

Author

Rizzo, Alberto, primary, Foschi, Antonella, additional, Bracchitta, Fiorenza, additional, Faggion, Ivano, additional, Salari, Federica, additional, Borgonovo, Fabio, additional, Fusetti, Chiara, additional, Mileto, Davide, additional, Rizzardini, Giuliano, additional, Lombardi, Alessandra, additional, Gismondo, Maria R., additional, and Micheli, Valeria, additional

Published
2022
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34. Concomitant diagnosis of sexually transmitted infections and human monkeypox in patients attending a sexual health clinic in Milan, Italy

Author

Rizzo, Alberto, primary, Pozza, Giacomo, additional, Salari, Federica, additional, Giacomelli, Andrea, additional, Mileto, Davide, additional, Cossu, Maria Vittoria, additional, Mancon, Alessandro, additional, Gagliardi, Gloria, additional, Micol, Bianchi, additional, Micheli, Valeria, additional, Capetti, Amedeo, additional, Antinori, Spinello, additional, Gismondo, Maria Rita, additional, Olivieri, Pietro, additional, and Lombardi, Alessandra, additional

Published
2022
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36. Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022

Author

Moschese, Davide, primary, Pozza, Giacomo, additional, Mileto, Davide, additional, Giacomelli, Andrea, additional, Cutrera, Miriam, additional, Cossu, Maria Vittoria, additional, Matone, Maddalena, additional, Beltrami, Martina, additional, Salari, Federica, additional, Antinori, Spinello, additional, Lombardi, Alessandra, additional, and Rizzardini, Giuliano, additional

Published
2022
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38. Comorbidity Burden and Suboptimal Immunological Responses to Coronavirus Disease 2019 Vaccination in People Living with Human Immunodeficiency Virus

Author

Cossu, Maria Vittoria, primary, Mileto, Davide, additional, Giacomelli, Andrea, additional, Oreni, Letizia, additional, Bracchitta, Fiorenza, additional, Pellicciotta, Martina, additional, Salari, Federica, additional, Petri, Francesco, additional, Meraviglia, Paola, additional, Antinori, Spinello, additional, Rizzardini, Giuliano, additional, and Ridolfo, Anna Lisa, additional

Published
2022
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39. Amiata donkey body conformation, udder characteristics, and their relationship with milk yield and quality.

Author

Salari, Federica, Altomonte, Iolanda, Boselli, Carlo, and Martini, Mina

Subjects
MILK quality, MILK yield, DONKEYS, CHEST (Anatomy), MILK contamination, TANNER graphs, STATISTICAL correlation, MILKFAT
Abstract

To date, no selective actions have been taken to improve milk traits in dairy donkeys, and the characteristics of the udder are not well defined in relation to the productive characteristics. This study aimed at increasing knowledge on Amiata dairy donkey body conformation, udder traits, and their relationship with milk yield and quality. Morphological, udder, and teat measurements and milk evaluations of 45 pluriparous jennies were carried out. The average wither height of the jennies was 126 cm and the chest girth was 148 cm; a large standard deviation of some body measurements was found. Forty-nine percent of the animals showed a moderately developed udder, while most of the jennies had symmetrical half-udders (96%) and the intermammary cleft was clearly visible in 53% of subjects. Correlation analysis indicated that bigger animals tend to have bigger udders, higher teat diameter, and greater distance between teat tips. A positive correlation between the teat length and the milk fat was found (p < 0.01), which suggests that jennies with longer teats have a better ability to release milk fat. The results of this paper may be useful to define the characteristics of the milking device and address selective choices of the animals. [ABSTRACT FROM AUTHOR]

Published
2023
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41. Concomitant diagnosis of sexually transmitted infections and human monkeypox in patients attending a sexual health clinic in Milan, Italy.

Author

Rizzo, Alberto, Pozza, Giacomo, Salari, Federica, Giacomelli, Andrea, Mileto, Davide, Cossu, Maria Vittoria, Mancon, Alessandro, Gagliardi, Gloria, Micol, Bianchi, Micheli, Valeria, Capetti, Amedeo, Antinori, Spinello, Gismondo, Maria Rita, Olivieri, Pietro, and Lombardi, Alessandra

Subjects
SEXUALLY transmitted diseases, COMORBIDITY, MONKEYPOX, SEXUAL health, TRICHOMONAS vaginalis, TRICHOMONIASIS, GONORRHEA
Abstract

In 2022, many monkeypox (MPX) outbreaks have been documented in countries where MPX was not endemic. It spread all around the world, especially in European Union and United States. While MPX is classically considered to be transmitted through close contact with lesions, the hypothesis of sexual transmission has been proposed. This study considered a total of 49 patients suspected for MPX that were also tested for other sexually transmitted infections (STIs), including Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, and Trichomonas vaginalis. The data from coinfected patients suggested that MPXV and STIs might share the same route of inoculum, corroborating the hypothesis of possible sexual transmission for the emerging poxvirus. And like any other STI, MPX should be considered without stigmatization. [ABSTRACT FROM AUTHOR]

Published
2023
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44. Characterization and Clonal Diffusion of Ceftaroline Non-Susceptible MRSA in Two Hospitals in Central Italy

Author

Morroni, Gianluca, primary, Fioriti, Simona, additional, Salari, Federica, additional, Brenciani, Andrea, additional, Brescini, Lucia, additional, Mingoia, Marina, additional, Giovanetti, Eleonora, additional, Pocognoli, Antonella, additional, Giacometti, Andrea, additional, Molinelli, Elisa, additional, Offidani, Annamaria, additional, Simonetti, Oriana, additional, and Cirioni, Oscar, additional

Published
2021
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46. Ongoing sporadic monkeypox cases: neutralising antibody detection in asymptomatic individuals

Author

ANTINORI, SPINELLO, BIANCHI, MICOL, CAPETTI, AMEDEO FERIDNANDO, CARUSO, FRANCESCO, COLOMBO, MARTINA LAURA, COSSU, MARIA VITTORIA, CUTRERA, MIRIAM, GISMONDO, MARIA RITA, GORI, ANDREA, LAZZARIN, SAMUEL, LOMBARDI, ALESSANDRA, MICHELI, VALERIA, PASTENA, ANDREA, POZZA, GIACOMO, RIZZARDINI, GIULIANO, RIZZO, ALBERTO, SABAINI, FEDERICO, SALARI, FEDERICA, Moschese, Davide, Giacomelli, Andrea, and Mileto, Davide

Published
2023
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