1. Accuracy ofindividual rapid tests for serodiagnosis of gambiense sleeping sickness in west Africa
- Author
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Jamonneau, Vincent, Camara, O., Ilboudo, H., Peylhard, M., Koffi, M., Sakande, H., N'Dri, L., Sanou, D., Dama, E., Camara, M., and Lejon, Veerle
- Subjects
parasitic diseases ,equipment and supplies - Abstract
Background Individual rapid tests for serodiagnosis (RDT) of human African trypanosomiasis (HAT) are particularly suited for passive screening and surveillance. However, so far, no large scale evaluation of RDTs has been performed for diagnosis of Trypanosoma brucei gambiense HAT in West Africa. The objective of this study was to assess the diagnostic accuracy of 2 commercial HAT-RDTs on stored plasma samples from West Africa. Methodology/Principal findings SD Bioline HAT and HAT Sero-K-Set were performed on 722 plasma samples originating from Guinea and Cote d'Ivoire, including 231 parasitologically confirmed HAT patients, 257 healthy controls, and 234 unconfirmed individuals whose blood tested antibody positive in the card agglutination test but negative by parasitological tests. Immune trypanolysis was performed as a reference test for trypanosome specific antibody presence. Sensitivities in HAT patients were respectively 99.6% for SD Bioline HAT, and 99.1% for HAT Sero-K-Set, specificities in healthy controls were respectively 87.9% and 88.3%. Considering combined positivity in both RDTs, increased the specificity significantly (p
- Published
- 2015