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Serpentinites associated with metamafic and metaplagiogranitic rocks occur sporadically within metasedimentary schists in the Yuli belt. Such metaigneous rocks are considered to represent ophiolitic protoliths and some contain high-pressure (HP) metamorphic minerals or assemblages. However, the origin and metamorphism of these serpentinites have long been a mystery. This study systematically investigates the four major serpentinite-bearing exposures at the Fengtien, Wanjung, Tsunkuanshan, and Chinshuichi. The results reveal that the serpentinites are of two different protoliths on the basis of relict chromian spinel composition. Cr-spinel compositions of the Fengtien and Tsunkuanshan samples are characterised by moderate Cr# [Cr/(Cr + Al)] (0.45–0.57), relatively high Mg# [Mg/(Mg+Fe2+)] (0.59–0.79), but low Fe3+# [Fe3+/(Fe3++Cr+Al)] (0.02–0.06), reflecting an abyssal peridotite type protolith. By contrast, Cr-spinel compositions of the Wanjung and Chinshuichi samples show high Cr# (up to 0.74) and Fe3+# (0.03–0.08), but low Mg# (<0.6), indicating a forearc mantle peridotite origin. Peak metamorphic conditions of the serpentinites are represented by the assemblage of antigorite + magnetite + chlorite + olivine + diopside, which is estimated as up to 550°C but the pressure cannot be constrained quantitatively. Based on field relations, it is inferred that the serpentinites, associated HP metaigneous rocks, and garnet-bearing metasedimentary schists were metamorphosed isofacially. Despite two origins, the peridotitic protoliths were all subjected to hydration and subduction processes. We suggest that precursors of the serpentinites, metaigneous rocks, and metasedimentary schists were juxtaposed and metamorphosed at intermediate to great depths (~35–55 km) of a subduction zone. Therefore, the Yuli belt serpentinites and associated rocks likely represent exhumed materials from a palaeo-subduction interface. [ABSTRACT FROM AUTHOR]