88 results on '"Saim, L."'
Search Results
2. Stem cell genes are poorly expressed in chondrocytes from microtic cartilage
- Author
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Ishak, M.F., Chua, K.H., Asma, A., Saim, L., Aminuddin, B.S., Ruszymah, B.H.I., and Goh, B.S.
- Published
- 2011
- Full Text
- View/download PDF
3. Surgical Outcome of Bone Anchored Hearing Aid (Baha) Implant Surgery: A 10 Years Experience
- Author
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Asma, A., Ubaidah, M. A., Hasan, Siti Salbiah, Wan Fazlina, W. H., Lim, B. Y., Saim, L., and Goh, B. S.
- Published
- 2013
- Full Text
- View/download PDF
4. Reconstruction of living bilayer human skin equivalent utilizing human fibrin as a scaffold
- Author
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Mazlyzam, A.L., Aminuddin, B.S., Fuzina, N.H., Norhayati, M.M., Fauziah, O., Isa, M.R., Saim, L., and Ruszymah, B.H.I.
- Published
- 2007
- Full Text
- View/download PDF
5. Internal auditory canal stenosis in congenital sensorineural hearing loss
- Author
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Sakina, M.S., Goh, B.S., Abdullah, A., Zulfiqar, M.A., and Saim, L.
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- 2006
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- View/download PDF
6. Long-term status of middle-ear aeration post canal wall down mastoidectomy
- Author
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Ezulia, T, primary, Goh, B S, additional, and Saim, L, additional
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- 2019
- Full Text
- View/download PDF
7. Magnonic Coherent Transport Inside the Inhomogeneous 2D-Hexagonal System
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Tigrine, R., primary, Saim, L., additional, and Khater, A., additional
- Published
- 2016
- Full Text
- View/download PDF
8. Status of Bilateral Cochlear Implantation in Malaysia
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Tan Sn and Saim L
- Subjects
medicine.medical_specialty ,Profound sensorineural hearing loss ,business.industry ,Cochlear implant ,medicine.medical_treatment ,medicine ,General Medicine ,Implant ,Cochlear implantation ,business ,Cochlear nucleus ,Surgery - Abstract
Advantages of bilateral Cochlear Implantation (CI) over unilateral implantation in individuals with severe to profound sensorineural hearing loss are well established. In most developed countries, cochlear implant recipients receive full funding for bilateral implantation. In Malaysia, we have been advocating bilateral implantation since 2003.Our objective is to review all cases of unilateral & bilateral implantation that used the Cochlear Nucleus implants (Cochlear Ltd. Australia) and determined the percentage of bilateral implantation & investigate their affordability and sources of funding. Data were collected from all individuals who underwent CI from 1995 until 2013 to determine bilateral or single sided recipients, sources of funding and time interval for both the first and the second implant. 510 individuals underwent CI from 1995 to 2013. Overall, only 34 individuals have bilateral implantation (6.7%). Amongst these, all except one were done after 2003 (93.3%).Time interval of the sequential implantation were 5 months as shortest interval and 15 years as longest interval with an average interval of 3 years. Majority of individuals received full or partial funding from the government for the first implantation. With regards to second implantation, 26 were self funded & 5 were government funding. Less than ten percent of cochlear implant recipients in Malaysia received bilateral implantation. Although bilateral CI is highly recommended, only a small number of individuals can afford bilateral implantation in Malaysia. With increasing advancement of this technology & Malaysia as one of the developing countries, thus advocating bilateral cochlear implant to the government should be strongly pursued.
- Published
- 2014
9. A foreign body granuloma in the neck mimicking metastatic tumour: a lesson to learn
- Author
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Putra, S.H.A. Primuharsa, Mazita, A., Abdullah, A., and Saim, L.
- Subjects
Tumors -- Diagnosis ,Tumors -- Care and treatment ,Foreign bodies (Medical care) -- Health aspects ,Health - Abstract
Table of Contents Abstract Introduction Case Report Discussion Conclusion References Abstract A considerable number of articles on retained gauze in the abdomen and thoracic have been reported in the literature. [...]
- Published
- 2007
10. Ear Infection and Hearing Loss Amongst Headphone Users
- Author
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Mazlan, R., Saim, L., Thomas, A., Said, R., and Liyab, B.
- Subjects
otorhinolaryngologic diseases ,Original Article ,sense organs - Abstract
The use of headphone has been thought to cause infection in the ear canal and contribute to hearing loss. In this study, we examined 136 Customer Service Representative from Celcom (Malaysia) Sdn. Bhd. who use headphone throughout their working hours. The purpose of this study was to determine the prevalence of ear canal infection and other related diseases of the ear, nose and throat. Their hearing thresholds were also determined using the Amplaid 309 Clinical Audiometer. We found no incidence of infection of the external ear canal amongst the subjects. There were 4 cases of chronic middle ear infection and 4 cases of impacted wax. Hearing impairment was found in 25 subjects (21.2%). However, there was no significant association between hearing loss and the exposure to sound from headphone usage because the high frequencies were not predominantly affected. There was also no association between hearing loss and duration of service.
- Published
- 2002
11. Cholesteatoma in patients with congenital external auditory canal anomalies: retrospective review
- Author
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Mazita, A, primary, Zabri, M, additional, Aneeza, W H, additional, Asma, A, additional, and Saim, L, additional
- Published
- 2011
- Full Text
- View/download PDF
12. MedEdWorld Online Global Collaborative Learning Network Using Adobe Connect: An Experience of Universiti Kabangsaan Malaysia Medical Centre
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Salam, Abdus, primary, Aziz, NA, additional, Arif, K, additional, Harlina, HS, additional, Norhayati, M, additional, and Saim, L, additional
- Published
- 2009
- Full Text
- View/download PDF
13. Metachronous inflammatory myofibroblastic tumour in the temporal bone: case report
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Goh, B S, primary, Tan, S P, additional, Husain, S, additional, Rose, I M, additional, and Saim, L, additional
- Published
- 2009
- Full Text
- View/download PDF
14. Preoperative High Resolution CT and MR Imaging in Cochlear Implantation
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Abdullah, A, primary, Mahmud, M R, additional, Maimunah, A, additional, Zulfiqar, M A, additional, Saim, L, additional, and Mazlan, R, additional
- Published
- 2003
- Full Text
- View/download PDF
15. Prevalence of otitis media with effusion amongst pre-school children in Malaysia
- Author
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Saim, A., primary, Saim, L., additional, Saim, S., additional, Ruszymah, B.H.I., additional, and Sani, A., additional
- Published
- 1997
- Full Text
- View/download PDF
16. Hearing aid use in special schools for the deaf in Malaysia
- Author
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Mukari, S.Z., primary, Ahmad, K., additional, Saim, L., additional, and Mohamed, A.S., additional
- Published
- 1997
- Full Text
- View/download PDF
17. Relation of distortion product otoacoustic emission with tinnitus.
- Author
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Ami M, Abdullah A, Awang MA, Liyab B, and Saim L
- Published
- 2008
18. Formation of tissue-engineered human auricular cartilage via tissue engineering technique for future use in ear surgery
- Author
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Saim L, Bs, Aminuddin, Munirah S, Kh, Chua, Izuddin Fahmy Abu, Nh, Fuzina, Isa MR, and Bh, Ruszymah
- Subjects
Microscopy ,Cartilage ,Chondrocytes ,Tissue Engineering ,Humans ,Cell Count ,Ear, External ,Cell Division ,Cells, Cultured ,Elastin - Abstract
To date there is no optimal approach to reconstruct an external ear. However, advances in tissue engineering technologies have indicated that in vitro autologous elastic cartilage might be of great importance in the future treatment of these patients. The aim of this study was to observe monolayer expansion of auricular cartilage and to evaluate engineered cartilage using standard histochemical study.
19. Effects of betahistine on patient-reported outcomes in routine practice in patients with vestibular vertigo and appraisal of tolerability: Experience in the OSVaLD study
- Author
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Benecke, H., Pérez-Garrigues, H., Bin Sidek, D., Uloziene, I., Kuessner, D., Sondag, E., Theeuwes, A., Boari, L., Chaves, A. G., Dorigueto, R. S., Ganança, F. F., Gonçalves, D. U., Hyppolito, M. A., Korn, G. P., Munhoz, M. S., Oliveira, J. A., Ajisafe, O., Angilleta, B., Bracalenti, I., Carlos, J. M., Dada, O., Ho, M., Kopyto, A., Laliberté, A., Lau, Y., Medina, X., Mercier, C., Nijmeh, P., Pietraszek, B., Roberge, C., Vincent, S., Zeitouni, A., Aras, I., Bencic, I., Bonifacic, M., Branica, S., Dovzak-Kokic, D., Drvis, P., Gortan, D., Grdinic, B., Grigic, J., Handzic, I., Ivkovic, M., Juros, V., Kovacic, J., Krstic, E., Lucin, Z., Maksimovic, Z., Maslovara, S., Rak, I., Resler Seks, A., Ries, M., Trotic, R., Rosenberg, A., Gaal, A., Badacsonyi, M., Balogh, G., Bandula, M., Baranya, E., Jeges, B., Brajnovits, T., Bucsai, A., Tubony, C., Csill, R., Czegledi, I., Olah, L., Draveczky, E., Vaszkun, L., Siro, E., Fain, A., Foth, A., Gerlinger, I., Gestelyi, G., Getachen, K., Ghayada, R., Gilincsek, L., Guth, I., Hegedus, E., Hegyi, I., Jofeju, E., Kerepesi, L., Krisan, I., Laszlo, K., Lorincz, T., Marisch, I., Mihalecz, K., Breznyan, M., Mori, I., Nagy, L., Manhalter, N., Pal, A., Papp, M., Peter, J., Prunk Eger, F., Radai, F., Szihalmy, I., Torma, E., Torok, K., Trencsenyi, G., Varga, E., Vincze, A., Vogel, R., Szakolczay, Z., Zsilinszky, Z., Rovo, Z., Tamas, L., Mester, B., Hudak, I., Toth, L., Merczel, A., Agarwal, V. K., Bhatia, R., Bhimani, B., Biswal, R. N., Biswas, A., Chowdary, V. S., Dhond, P., Dube, T. N., Gopakumar, G., Kansara, A., Khound, G., Kirtane, M. V., Mukherjee, A., Nagpal, T., Ravikumar, A., Reddy, V., Sampat, N., Shaikh, S., Sinha, S., Vaid, N., Valsangkar, S., Vasnoi, S., Vishwanathan, A., Blumberga, I., Bucina, B., Cakule, G., Demidova, L., Dolge, A., Dzirgause, M., Freimane, A., Fricbergs, J., Frolova, V., Ganus, I., Gavare, I., Grigs, V., Grusle, M., Levins, E., Veidule, I., Indrane, M., Saihulova, I., Jeca, A., Jegere, D., Ivanova, A., Kalitas, N., Kalnina, Z., Kanepe, K., Karlovska, M., Kokina, I., Krigere, R., Krisjane, D., Kukurane, S., Kundrate, G., Kukaine, S., Kukute, I., Lagzdina, L., Lapsa-Arenta, S., Madre, S., Matusevica, A., Mežale, I., Melnika, V., Mickevica, S., Morlata, N., Naudina, M. S., Nimroda, L., Norina, D., Opelte, V., Pavlovska, I., Priede, Z., Proskurna, T., Purina, J., Kamsa, I., Raumane, D., Kenina, V., Roska-Levina, D., Rozenbaha, A., Rozkalne, A., Ruta, A., Sendze, G., Silins, A., Skrupska, D., Skurule, I., Sokalska, A., Stepko, Z., Supe, I., Telezenko, I., Tretjaka, N., Turlaja, V., Uzbeka, I., Valucka, T., Vancans, J., Vasilevskis, U., Veinberga, V., Viba, Z., Vitkovska, M., Vitolina, A., Voitovica, L., Zigure, I., Zilite, I., Bakstiene, J., Balkaitiene, R., Basinskiene, V., Beinaraviciene, R., Bertasiene, Z., Bieliauskiene, I., Budrikiene, N., Butkus, A., Butkus, E., Butkus, R., Cholomskiene, V., Dainius, K., Degteriova, R., Deveikyte, A., Dirzauskiene, J., Einoriene, D., Gadeikis, E., Gircys, P., Grazeviciute, L., Ivaskevicius, A., Janciute, J., Jankauskiene, D., Jersova, J., Jociene, I., Jokimaitiene, J., Jukneliene, R., Kanapeckiene, V., Karaliene, V., Kazlauskas, A., Kicas, R., Kiskuniene, I., Kiudelis, A., Kizlaitiene, R., Kuriene, A., Lukaseviciene, N., Lukosaitis, A., Malikeniene, T., Markeleviciene, R., Mazonyte, S., Nadusauskiene, M., Narkeviciene, V., Naumcik, J., Navickiene, E., Pancyreva, I., Pavydyte, J., Persidskaja, O., Petkiniene, V. R., Petrileviciene, R., Petrosiute, B., Pliopliene, I., Puckiene, Z., Razukiene, J., Remeikiene, S., Rudzeviciene, E., Sceponaviciute, S., Scerbickiene, L., Sersniova, I., Sinkuniene, N., Skerneviciute, I., Snureviciute, V., Sostakiene, N., Tunkulas, E., Vitkauskiene, V., Zakarauskiene, R., Zorjan, N., Zurauskiene, R., Sani, A., Mohamad, A., Abdullah, A., Abdullah, B., Hassan, F., Selvarajah, G., See, G. B., Mann, G. S., Singh, H., Hj Ahmad, H. A., Hailani, I., Mohd Yusof, I., Gopalan Nair, K., Sathananthar, K. S., Singh, K., Saim, L., Abdul Ghani, M. H., Herg, M., Jalaludin, M. A., Md Daud, M. K., Khir Abdullah, M., Noor, N. H., Mohamed, N. R., Esa, N. K., Jusoh, N. M., Narayanan, P., Choo, P. K., Al Konee, R. A., Rajagopalan, R., Ismail, R., Mohd Hashim, S. S., Kumarasamy, S., Suan, T. L., Kamalden, T. M., Sang, T. T., Ambu, V. K., Leman, W. I., Abidin, Z. A., Salahuddin, Z., Yusof, Z., Burduk, P., Chmura, H., Czecior, E., Dabrowski, P., Diechota, L., Dietrich, G., Domagata, M., Durko, M., Frak, W., Franczuk-Gwiazda, M., Galbarczyk, D., Gaweowicz, J., Kabacinska, A., Kadej, G. Z., Kapuscinski, J., Kolebacz, B., Korpus-Kaminska, I., Lachowicz, M., Mielnik, E., Mihutka, S., Nilewski, J., Nouinska, E., Obzebowska-Karszania, Z., Oleksiak, M., Palasik, W., Paradowski, B., Paskal, J., Pospiech, L., Pres, K., Rynio, E., Schneider, K., Siger, M., Stoniewska-Piackus, M., Szczuto, J., Wilczynski, K., Wojcick, P., Woynowski, W., Tomasz Zatonski, Ziolkowska-Kochan, M., Zygadlo, E. N., Alaicescu, M., Augustin, A., Bădescu, A., Baltag, D., Bărbos, C., Becuşi, T., Bucan, L., Călăraşu, R., Cămpeanu, A., Chirileanhu, R. D., Comşa, G. I., Constantinescu, D., Cotulbea, S., Cozma, S., Cucoş, L., Docu, A. A., Dulămea, A., Enache, N., Ene, A., Fischer, T. S., Floare, L., Frăsineanu, A., Geană, I., Georgescu, E., Georgescu, M., Georgescu, M. J., Gherman, E., Hăncu, A., Iliescu, I., Ionescu-Mihăiţă, E. R., Ionita, E., Ionita, I., Iovănescu, D., Ladea, M., Loghin, V., Marceanu, L., Mărginean, I., Mariam, G., Marin, M., Mariş, C., Mârţu, D., Matcău, L., Muhlfay, G., Muică, L., Naconecinîi, D., Nirestean, A., Niţă, A., Niţu, L., Oană, N., Oancea, A., Oşanu, M., Panea, N., Pascu, A., Pastia, M., Pavel, R., Pendefunda, L., Petrutiu, S., Plăviţu, I., Poenaru, M., Popa, G. C., Popa, G., Popi, S., Popovivci, A., Prelipceanu, D., Radu, L., Rădulescu, L., Roceanu, A., Rusu, A., Sabău, M. S., Safta, D., Sarafoleanu, D., Stanciu, M., Stănciulescu, R., Ştefanache, F., Stefanescu, E. H., Szatmari, S., Szocs, Tomescu, L., Tudorache, B., Tudose, C., Ursu, C., Vasilescu, L., Vasu, I., Vioreanu, M., Zaboş, D., Zaharia, C., Zainea, V., Zarie, G., Alekseeva, N., Amelin, A., Artemova, I., Batysheva, T., Bobyreva, S., Boyko, A., Buldakova, N., Ganzhula, P., Gaponova, O., Hanevich, T., Hozova, A., Isachenkova, O., Ismailov, A., Zhuravleva, E., Kostenko, E., Lilenko, S., Lisenker, L., Makarova, G., Manevich, T., Matsnev, E., Melnikov, O., Morozova, S., Nesterova, O., Nikulina, I., Otcheskaya, O., Pivovarova, V., Rotor, L., Rylskiy, A., Shalabanova, I., Shinkarev, S., Sorokoumov, V., Vdovichenko, T., Vinetskiy, Y., Vostricova, I., Zadorozhnaya, T., Breznik Farkas, B., Felbabic, J., Geczy Buljovcic, B., Grad, A., Hoenigman, B., Kurent, Z., Krek, B. P., Rok, B., Spindler, M., Vatovec, J., Zorn, A., Zupan, L., Aguila, A. A., Caballero, B. M., Garcia, V. C., Cruellas, T. F., Munoz, P. C., Domenech, J. I., Donderis, S. J., Estevez, G. M., Pallas, P. E., Grani, M. F., Gonzalez, C. F., Galindo, O. J., Garcia, A. A., Garcia, G. B., Gonzalez, G. I., Hernandez Ade, S., Hijano, E. R., Lopez, E. J., Saiz, A. J., Izquierdo, L. J., Perez, L. L., Paya, P. L., Mesa, M. M., Molina, P. M., Navarrete, A. L., Marti, G. L., Melgarejo, M. F., Orts, A. M., Suarez, G. P., Perez, M. P., Perez, G. H., Perez, G. V., Rodriguez, R. S., Santos, P. S., Soto, V. A., Malluguiza, C. J., Ramirez, L. R., Jimenez, M. R., Barona Guzmán, R., Escamilla, C. Y., Saiz, M. V., Gisbert, A. F., Provedo, P. C., Pardo, S. E., Alemán, L. O., Martin, S. E., and Marco, A. J.
20. Technology enhanced global online collaborative networking using MedEdWorld Wimba: UKM medical centres' experience
- Author
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Salam, A., Ibrahim, N. M., Kamaruddin, M. A., Besar, M. N. A., Siraj, H. H., Mohamad, N., Mahdy, Z. A., Ali Azman Raymond, and Saim, L.
21. Formation of tissue-engineered human auricular cartilage via tissue engineering technique for future use in ear surgery
- Author
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Saim, L., Aminuddin, B. S., Munirah, S., Chua Kien Hui, Izuddin Fahmy, A., Fuzina, N. H., Isa, M. R., and Ruszymah, B. H.
22. Histological Analysis of Tissue Engineered Cartilage Made from Microtic Auricular Chondrocytes.
- Author
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Goh, B. S., Ishak, M. F., Chua, K. H., Asma, A., Saim, L., Aminuddin, B. S., and Ruszymah, B. H. I.
- Published
- 2010
23. Growth Factor Supplementation Does Not Maintain Hair Cell Specific Genes in Cochlear Culture.
- Author
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Ubaidah, M. A., Chua, K. H., Mazita, A., Zainal, A., Aminuddin, B. S., Ruszymah, B. H. I., and Saim, L.
- Published
- 2010
24. Perception of telemedicine among medical practitioners in Malaysia during COVID-19.
- Author
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Thong HK, Wong DKC, Gendeh HS, Saim L, Athar PPBSH, and Saim A
- Subjects
- Cross-Sectional Studies, Female, Humans, Malaysia epidemiology, Male, Pandemics, Perception, SARS-CoV-2, COVID-19, Surgeons, Telemedicine
- Abstract
The novel Coronavirus Disease 2019 (COVID-19) has brought unprecedented changes in the way conventional health care is delivered. This study examined if clinicians' perceptions regarding telemedicine and its barriers to implementation in Malaysia have changed during this pandemic. A cross-sectional survey was conducted among Malaysian medical doctors of various specialties in four urban healthcare facilities between June 2020 and July 2020. A total of 146 (41.7%) out of 350 responses were obtained. 62% of doctors reported a reduction greater than 50% in outpatient visits during the COVID-19 pandemic. The majority of doctors either found telemedicine useful in situations similar to COVID-19 (34.2%) or that it is essential to their daily practice (42.5%). However, only 22% reported using telemedicine for consultation during the COVID-19 pandemic. 74% of doctors felt that telemedicine would only benefit up to 30% of their patient population. Significantly more female doctors (80%) felt that telemedicine would benefit their patients compared to male doctors (45.8%) (P=0.03). Physicians (51.3%) were more inclined to adopt telemedicine in comparison to surgeons (32.4%) (P=0.03). The majority cited medico-legal issues and consent (80.6%), billing and charges (66.7%) and insurance reimbursement (62.5%), technical difficulties (62.5%) as their barrier to the adoption of telemedicine. Female doctors and physicians were more willing to adopt telemedicine when compared to male doctors and surgeons. Although the COVID-19 pandemic appeared to improve the perception, significant barriers should be resolved before many can incorporate it into their practice., (©2021 JOURNAL of MEDICINE and LIFE.)
- Published
- 2021
- Full Text
- View/download PDF
25. The immediate effect of facial candling on inflammatory mediators, substance P, symptoms severity, and quality of life in allergic rhinitis patients: Study protocol for a randomized controlled trial.
- Author
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Ismail NFF, Neoh CF, Lim SM, Abdullah AH, Mastuki MF, Ramasamy K, Zainuddin N, Saim L, and Ming LC
- Subjects
- Academic Medical Centers, Adolescent, Adult, Aged, Biomarkers metabolism, Humans, Malaysia, Middle Aged, Mucus metabolism, Nasal Cavity, Quality of Life, Severity of Illness Index, Time Factors, Treatment Outcome, Young Adult, Medicine, East Asian Traditional, Rhinitis, Allergic immunology, Rhinitis, Allergic therapy, Substance P metabolism
- Abstract
Introduction: Asian countries have a variety of ethnic groups and culture that provide their own traditional treatment in health care. Facial candling appears to be one of the popular traditional treatments in Southeast Asian. The complementary medicine practitioners promote that the facial candling treatment would help in reducing the symptoms of allergic rhinitis and other problems related to sinus. Due to the lack of evidence available, the effectiveness of this treatment method and its mechanism, however, remains unknown. The objective of this research is therefore to study impact of facial candling on inflammatory mediators, substance P (SP), symptoms severity, and quality of life (QoL) in allergic rhinitis patients., Method and Analysis: A randomized, nonblinded, controlled trial will be carried out by recruiting a total of 66 eligible allergic rhinitis patients who fulfill the inclusion criteria from a university health center. The subjects will be randomly assigned into 2 groups: intervention group receiving facial candling treatment and control group (no treatment given). Samples of blood and nasal mucus will be collected right before and after intervention. Samples collected will be analyzed. The primary outcomes are the changes in the level of SP in both blood and mucus samples between both groups. The secondary outcomes include the levels of inflammatory mediators (ie, tumor necrosis factor alpha, interleukin (IL)-3, IL-5, IL-6, IL-10, and IL-13) and the severity of allergic rhinitis symptoms as measured by a visual analogous scale and QoL using the Rhinitis Quality of Life Questionnaire (RQLQ)., Ethical and Trial Registration: The study protocols are approved from the Ethical and Research Committee of the Universiti Teknologi MARA (REC/113/15). The trial is registered under the Australia New Zealand Clinical Trial Registry (ACTRN12616000299404). The trial was registered on 03/07/2016 and the first patient was enrolled on 10/12/2016., Conclusion: Facial candling is one of the unique treatments using candles to reduce the severity of symptoms and inflammation. This is the first ever study conducted on facial candling that will give rise to new knowledge underlying the effects of facial candling on severity of symptoms and inflammation relief mechanism mediated by substance P and inflammatory mediators.
- Published
- 2017
- Full Text
- View/download PDF
26. Chondrogenesis of human adipose derived stem cells for future microtia repair using co-culture technique.
- Author
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Goh BS, Che Omar SN, Ubaidah MA, Saim L, Sulaiman S, and Chua KH
- Subjects
- Adipose Tissue cytology, Biomarkers metabolism, Congenital Microtia surgery, Gene Expression, Humans, Primary Cell Culture, Transforming Growth Factor beta, Adult Stem Cells physiology, Chondrogenesis, Coculture Techniques
- Abstract
Conclusion: In conclusion, these result showed HADSCs could differentiate into chondrocytes-like cells, dependent on signaling induced by TGF-β3 and chondrocytes. This is a promising result and showed that HADSCs is a potential source for future microtia repair. The technique of co-culture is a positive way forward to assist the microtia tissue., Objective: Reconstructive surgery for the repair of microtia still remains the greatest challenge among the surgeons. Its repair is associated with donor-site morbidity and the degree of infection is inevitable when using alloplastic prosthesis with uncertain long-term durability. Thus, human adipose derived stem cells (HADSCs) can be an alternative cell source for cartilage regeneration. This study aims to evaluate the chondrogenic potential of HADSCs cultured with transforming growth factor-beta (TGF-β) and interaction of auricular chondrocytes with HADSCs for new cartilage generation., Methods: Multi-lineages differentiation features of HADSCs were monitored by Alcian Blue, Alizarin Red, and Oil Red O staining for chondrogenic, adipogenic, and osteogenic differentiation capacity, respectively. Further, HADSCs alone were culture in medium added with TGF-β3; and human auricular chondrocytes were interacted indirectly in the culture with and without TGF-βs for up to 21 days, respectively. Cell morphology and chondrogenesis were monitored by inverted microscope. For cell viability, Alamar Blue assay was used to measure the cell viability and the changes in gene expression of auricular chondrocyte markers were determined by real-time polymerase chain reaction analysis. For the induction of chondrogenic differentiation, HADSCs showed a feature of aggregation and formed a dense matrix of proteoglycans. Staining results from Alizirin Red and Oil Red O indicated the HADSCs also successfully differentiated into adipogenic and osteogenic lineages after 21 days., Results: According to a previous study, HADSCs were strongly positive for the mesenchymal markers CD90, CD73, CD44, CD9, and histocompatibility antigen. The results showed HADSCs test groups (cultured with TGF-β3) displayed chondrocytes-like cells morphology with typical lacunae structure compared to the control group without TGF-β3 after 2 weeks. Additionally, the HADSCs test groups increased in cell viability; an increase in expression of chondrocytes-specific genes (collagen type II, aggrecan core protein, SOX 9 and elastin) compared to the control. This study found that human auricular chondrocytes cells and growth factor had a positive influence in inducing HADSCs chondrogenic effects, in terms of chondrogenic differentiate of feature, increase of cell viability, and up-regulated expression of chondrogenic genes.
- Published
- 2017
- Full Text
- View/download PDF
27. Age and Gender Effects on Wideband Absorbance in Adults With Normal Outer and Middle Ear Function.
- Author
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Mazlan R, Kei J, Ya CL, Yusof WN, Saim L, and Zhao F
- Subjects
- Acoustic Stimulation, Adult, Aged, Aged, 80 and over, Aging radiation effects, Asian People, Biomechanical Phenomena, Ear, External radiation effects, Ear, Middle radiation effects, Female, Hearing Tests, Humans, Male, Middle Aged, Young Adult, Aging physiology, Ear, External physiology, Ear, Middle physiology, Sex Characteristics, Sound
- Abstract
Purpose: This study examined the effects of age and gender on wideband energy absorbance in adults with normal middle ear function., Method: Forty young adults (14 men, 26 women, aged 20-38 years), 31 middle-aged adults (16 men, 15 women, aged 42-64 years), and 30 older adults (20 men, 10 women, aged 65-82 years) were assessed. Energy absorbance (EA) data were collected at 30 frequencies using a prototype commercial instrument developed by Interacoustics., Results: Results showed that the young adult group had significantly lower EA (between 400 and 560 Hz) than the middle-aged group. However, the middle-aged group showed significantly lower EA (between 2240 and 5040 Hz) than the young adult group. In addition, the older adult group had significantly lower EA than the young adult group (between 2520 and 5040 Hz). No significant difference in EA was found at any frequency between middle-aged and older adults. Across age groups, gender differences were found with men having significantly higher EA values than women at lower frequencies, whereas women had significantly higher EA at higher frequencies., Conclusions: This study provides evidence of the influence of gender and age on EA in adults with normal outer and middle ear function. These findings support the importance of establishing age- and gender-specific EA norms for the adult population.
- Published
- 2015
- Full Text
- View/download PDF
28. Effect of Growth Factor Supplementation on the Hair Cell Specific Markers of Cells Harvested from Basilar Membrane.
- Author
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Ubaidah MA, Chua KH, Ami M, Zainal A, Saim A, Saim L, Lokanathan Y, and Haji Idrus RB
- Subjects
- Animals, Cell Differentiation drug effects, Cells, Cultured, Culture Media, Hair Cells, Auditory cytology, Immunohistochemistry, Mice, Basilar Membrane cytology, Hair Cells, Auditory metabolism, Intercellular Signaling Peptides and Proteins pharmacology
- Abstract
Objective: Loss of auditory hair cells is a major cause of deafness. The presence of auditory progenitor cells in the inner ear raises the hope for mammalian inner ear cell regeneration. In this study, we aimed to investigate the effect of growth factor supplementations, namely a combination of epidermal growth factor (EGF), insulin-like growth factor (IGF), and beta (β)-fibroblast growth factor (βFGF), on the expression of hair cell-specific markers by cells harvested from the cochlear membrane. This would provide an insight into the capability of these cells to differentiate into hair cells., Materials and Methods: EGF, IGF, and βFGF were supplemented into the culture medium. The cells were evaluated by morphology, growth kinetic, gene expression, and protein expression., Results: The cultured cells of mouse basilar membrane were spindle shaped. Growth factors-enriched medium promotes a significantly higher proliferative activity than the basic culture medium but did not alter the cell morphology. Growth factors-enriched medium did not show any significant differences in the protein expression of the hair cell-specific markers myosin VIIa and calretinin and the stem-cell marker nestin. Gene expression analysis showed that the expression of the hair cell-specific genes myosin VIIa and calretinin as well as the stem cell genes nestin, Rex1, and Sox2 was reduced after the cells were passaged in the growth factor-supplemented medium. Cells in the basic medium expressed a significantly higher level of hair cell-specific genes at certain passages., Conclusion: Growth factor supplementation could not maintain the expression of hair cell-specific markers by cells obtained from the cochlear membrane.
- Published
- 2015
- Full Text
- View/download PDF
29. The association between GJB2 mutation and GJB6 gene in non syndromic hearing loss school children.
- Author
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Asma A, Ashwaq A, Norzana AG, Atmadini AM, Ruszymah BH, Saim L, and Wahida IF
- Subjects
- Adolescent, Child, Connexin 26, Connexin 30, Female, Hearing Loss, Sensorineural ethnology, Humans, Malaysia, Male, Retrospective Studies, Asian People genetics, Connexins genetics, Hearing Loss, Sensorineural genetics, Mutation genetics
- Abstract
Recently, molecular testing for GJB2 mutations has become the standard of care for the diagnosis of patients with non syndromic hearing impairment of unknown cause. The aims of this study are to determine the association between GJB2 mutation and GJB6 and to report the variation of mutations in deaf students who have heterozygous GJB2. This retrospective study was conducted at Universiti Kebangsaan Malaysia Medical Center (UKMMC). Data was collected from previous files and records from Tissue Engineering and Human Genetic Research Group Laboratory. Approval from Ethical Committee was obtained prior to the study. A total of 138 students have been screened in previous studies in UKMMC for the presence of GJB2 mutations as a cause for hearing loss. Thirty four of the 138 subjects have GJB2 mutations; 2 showed homozygous mutations whereas another 32 were heterozygous for GJB2 gene mutation. Only 31 DNA samples of students presented with sensorineural hearing loss with heterozygous mutation in GJB2 gene were included in this study. The sequencing results obtained were analyzed. The degree of hearing loss of those students with association between GJB2 mutation and GJB6 mutation will be discussed. Five out of 31 subjects (16.2%) have mutations in their GJB6 gene, suggesting a digenic inheritance of GJB2/GJB6 mutation. In total, four novel mutations were identified; E137D (n=1), R32Q (n=1), E101K (n=1) and Y156H (n=1) and one mutation deletion; 366delT (n=1). All students with association GJB2 mutation and GJB6 showed severe to profound hearing loss in both ears. Interestingly this study not detected the large deletion of 342 kb in GJB6 gene suggesting that the mutation is very rare in this region compared to certain parts of the world.
- Published
- 2011
30. Mastoid abscess in acute and chronic otitis media.
- Author
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Ami M, Zakaria Z, Goh BS, Abdullah A, and Saim L
- Abstract
Background: Mastoid abscess remains a recognised complication of otitis media despite the advent of antibiotics. The objectives of this study were to describe the risk factors in patients with mastoid abscess following acute and chronic otitis media and discuss the management of this infection., Method: A retrospective analysis was done on all patients who underwent mastoidectomy for mastoid abscess from January 2002 to December 2007. Data on the patients' presentation, associated complications, management, and follow-up were analysed., Results: A total of 12 patients were enrolled in this study population. Group A consisted of patients with mastoid abscess preceded by acute otitis media, while Group B consisted of patients with mastoid abscess and chronic otitis media. In Group A (n = 7), 4 patients had a pre-morbid immunocompromised condition, but they did not have cholesteatoma. None of the patients in Group B (n = 5) had any pre-morbid illnesses. Out of 12 patients, 7 patients had associated extracranial complications, and 1 patient had intracranial complications. Most patients recovered well after mastoidectomy. Recurrence was noted in 1 patient who had acute lymphoblastic leukaemia., Conclusion: Mastoid abscess is still a recognised complication of acute otitis media, especially in patients who are immunocompromised. Immunocompetent patients may also develop mastoid abscess following chronic otitis media associated with cholesteatoma. Thus, early treatment of otitis media and close vigilant follow-up are advocated to ensure prompt detection of mastoid abscess complications.
- Published
- 2010
31. Congenital cholesteatoma: delayed diagnosis and its consequences.
- Author
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Goh BS, Faizah AR, Salina H, Asma A, and Saim L
- Subjects
- Adolescent, Child, Child, Preschool, Cholesteatoma, Middle Ear complications, Cholesteatoma, Middle Ear surgery, Female, Humans, Male, Cholesteatoma, Middle Ear congenital, Cholesteatoma, Middle Ear diagnosis, Delayed Diagnosis adverse effects, Hearing Loss etiology
- Abstract
This is a retrospective review of congenital cholesteatoma cases that were managed surgically. There were 5 cases. The age of presentation ranged from 5 to 18 year old. Three patients presented with complication of the disease. Three patients had intact tympanic membrane, two had perforation at the anterior superior quadrant. All patients had cholesteatoma medial to tympanic membrane. Four cases had extensive ossicular erosion with preoperative hearing worse than 40 dB. Four cases underwent canal wall down mastoid surgery and one underwent canal wall up surgery. One patient had recurrence which required revision surgery. In conclusion, congenital cholesteatoma presented late due to the silent nature of disease in its early stage. Extensive disease, ossicular destruction with risk of complication at presentation were marked in our study. Hence, more aggressive surgical intervention is recommended in the management of congenital cholesteatoma.
- Published
- 2010
32. Otosclerosis and the role of second ear surgery.
- Author
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Azlan II, Asma A, and Saim L
- Subjects
- Hearing, Hearing Loss, Humans, Tinnitus, Otosclerosis, Stapes Surgery
- Abstract
Hearing loss and tinnitus are the main symptoms of otosclerosis. Little is known about the cause of tinnitus in otosclerosis and the factors influencing the effect of surgery on tinnitus. Though by surgery, we are able to inform patient about probable hearing gain and even benefit concerning bilateral hearing, it is however difficult to predict the course of tinnitus. The principle aim of stapes surgery is to restore hearing but some patients also report reduction in the severity of tinnitus and even complete cessation of it. We describe a case report of a 37 year old male who underwent a second stapes surgery. We wish to illustrate that for our patient, tinnitus represents a major disturbance and the patient is as much concerned with the improvement of hearing as with the improvement of tinnitus.
- Published
- 2010
33. Iatrogenic facial nerve palsy: lessons to learn.
- Author
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Asma A, Marina MB, Mazita A, Fadzilah I, Mazlina S, and Saim L
- Subjects
- Facial Paralysis diagnostic imaging, Humans, Otologic Surgical Procedures adverse effects, Reoperation, Retrospective Studies, Tomography, X-Ray Computed, Facial Paralysis etiology, Facial Paralysis therapy, Iatrogenic Disease, Mastoid surgery
- Abstract
Introduction: This study aims to review the management and discuss the outcome of patients with iatrogenic facial nerve palsy., Methods: 11 patients with iatrogenic facial nerve palsy (FNP) were evaluated retrospectively in a tertiary centre between June 1995 and September 2008. All the cases were referred from other centres., Results: Ten patients had iatrogenic immediate FNP secondary to mastoidectomy and one had FNP secondary to superficial parotidectomy. Of the ten cases, three had concomitant profound sensorineural hearing loss and one had concomitant labyrinthine fistula. Ten patients underwent facial nerve exploration and one patient was managed conservatively. The second genu was the commonest site of injury (60 percent). Facial nerve recoveries were achieved to Grade I House Brackmann classification in five cases, Grade II in two cases and Grade III in two cases postoperatively. One case defaulted follow-up. One patient, managed conservatively, recovered to FNP Grade II after five months post-injury., Conclusion: Mistakes that most likely occurred during mastoid surgery are drilling towards the antrum, causing injury to the facial nerve at the second genu. Early facial nerve exploration and neurolysis resulted in good facial nerve recovery.
- Published
- 2009
34. Hearing rehabilitation in congenital canal atresia.
- Author
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Mazita A, Fazlina WH, Abdullah A, Goh BS, and Saim L
- Subjects
- Adolescent, Adult, Audiometry, Child, Child, Preschool, Ear Canal pathology, Female, Hearing Disorders pathology, Humans, Male, Osseointegration, Retrospective Studies, Ear abnormalities, Ear Canal abnormalities, Hearing, Hearing Aids, Hearing Disorders surgery
- Abstract
Introduction: The purpose of this study was to review the results of our patients with congenital canal atresia after implantation of bone-anchored hearing aids (BAHA). The occurrence of complications was also reviewed., Methods: This was a retrospective analysis of the first 16 patients who had BAHA implantation at Universiti Kebangsaan Malaysia Medical Centre, Malaysia. Audiometric assessment was done preoperatively and postoperatively for each patient using the standard procedure. The surgical procedure was described and its complications discussed., Results: The 16 patients consisted of 11 male and five female patients. Their mean age was 8.9 years at the time of the surgery. The main indication was bilateral canal atresia. 11 patients had implantation of BAHA performed in two stages, while the other five patients had it as a single-staged procedure. The complications that occurred were failure of osseointegration (one patient), granulation tissue overgrowth into the abutment (two patients) and cellulitis surrounding the abutment (three patients). The average preoperative unaided air conduction threshold was 64.9 dB and the average postoperative aided hearing threshold was 29.7 dB. The overall mean functional gain was 35.2 dB., Conclusion: BAHA has many advantages over the conventional hearing aid in the form of cosmesis, discomfort and hearing gain. It is a reliable hearing rehabilitation tool with good predictable hearing outcome in patients with bilateral canal atresia, especially those unsuitable for canalplasty. Despite its higher cost and the need for surgical implantation, its use is justifiable in properly selected patients.
- Published
- 2009
35. Human serum is an advantageous supplement for human dermal fibroblast expansion: clinical implications for tissue engineering of skin.
- Author
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Mazlyzam AL, Aminuddin BS, Saim L, and Ruszymah BH
- Subjects
- Adult, Animals, Cattle, Cell Cycle physiology, Cell Shape, Cell Survival, Cells, Cultured, Fibroblasts cytology, Gene Expression, Humans, Cell Culture Techniques, Fibroblasts physiology, Serum metabolism, Skin cytology, Tissue Engineering
- Abstract
Background: Standard fibroblast culture medium usually contains fetal bovine serum (FBS). In theory, unknown risks of infection from bovine disease or immune reaction to foreign proteins may occur if standard culture method is used for future human tissue-engineering development. Human serum (HS) theoretically would be another choice in providing a safer approach and autologous clinically reliable cells., Methods: Isolated human dermal fibroblasts were culture-expanded in an equal volume mixture of Ham's F12 medium and Dulbecco's Modified Eagle Medium (DMEM) supplemented with either 10% HS or 10% FBS from passage 0 to passage 3. Effects of 10% HS and 10% FBS on human fibroblast viability, growth kinetics, cell cycle analysis and gene expressions were investigated and compared., Results: Generally, fibroblast viability cultured in HS supplementations was much higher compared to FBS supplementation. Fibroblast proliferations were faster in HS supplementations with shorter doubling time. Cell cycle analysis showed fibroblasts cultured with HS supplementations have higher S-phase ratio compared to FBS. Gene expression levels by quantitative reverse transcriptase-polymerose chain reaction (RT-PCR) showed cultured fibroblasts with HS supplementation maintains expression of collagen type I collagen, increased expression of type III collagen and fibronectin and reduced expression of alpha-smooth muscle actin (alpha-SMA) compared to FBS., Conclusions: Results demonstrated potential advantages of HS vs. FBS in generating larger numbers of cultured dermal fibroblasts in a shorter period of time. HS also influenced mRNA expression of type III collagen and fibronectin (upregulated) and alpha-SMA (downregulated), which are important extracellular matrix proteins in wound healing.
- Published
- 2008
- Full Text
- View/download PDF
36. Benefit and pitfalls of newborn hearing screening.
- Author
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Asma A, Wan Fazlina WH, Almyzan A, Han YS, Jamilah AG, Roslin S, Ann MT, Borhan L, Wan Norliana A, Saim L, and Rohana J
- Subjects
- Cross-Sectional Studies, Evoked Potentials, Auditory, Brain Stem, Hearing Loss diagnosis, Humans, Infant, Infant, Newborn, Otoacoustic Emissions, Spontaneous, Referral and Consultation, Hearing Tests, Neonatal Screening
- Abstract
The importance of universal newborn hearing screening (UNHS) in identifying hearing-impaired infants as early as possible is already well recognized. Transient evoked otoacoustic emissions (TEOAE) have been established as a reliable method for UNHS in full term infants. This is a cross sectional study between April 2003--December 2005. Thirteen thousand five hundred and ninety eight (13,598) newborns were screened for hearing loss with portable otoacoustic emission (OAE) before discharge. The initial coverage rate during the 3 years study period was 85.9% (13,598) with 89.2% (3762), 79.0% (4480) and 90.3% (5356) for 2003, 2004 and 2005 respectively. The mean age when hearing loss was diagnosed using ABR were 3.56 months old, 3.08 months old, and 2.25 months old and 3.01 months old for 2003, 2004, 2005 respectively and it was statistically significant. The defaulter rate at the third stage during the 3 years study period was 35% (21), 15.2% (7) and 18.2% (2) for 2003, 2004 and 2005 respectively. This study showed significant improvement in initial referral rate, coverage rate and age of diagnosis. However, we need to improve on high defaulter rates.
- Published
- 2008
37. Metastasis to the sinonasal tract from sigmoid colon adenocarcinoma.
- Author
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bin Sabir Husin Athar PP, bte Ahmad Norhan N, bin Saim L, bin Md Rose I, and bte Ramli R
- Subjects
- Adenocarcinoma metabolism, Adenocarcinoma radiotherapy, Carcinoembryonic Antigen metabolism, Fatal Outcome, Female, Humans, Keratin-20 metabolism, Middle Aged, Palliative Care, Paranasal Sinus Neoplasms metabolism, Paranasal Sinus Neoplasms radiotherapy, Adenocarcinoma secondary, Colorectal Neoplasms pathology, Paranasal Sinus Neoplasms secondary
- Abstract
Introduction: Metastatic adenocarcinoma from the gastrointestinal tract to the sinonasal tract is rare. The histological morphology of this lesion is indistinguishable from the colonic variant of primary sinus adenocarcinoma or intestinal-type adenocarcinoma (ITAC)., Clinical Picture: This is a report of a case of metastatic adenocarcinoma of colorectal origin to the paranasal sinuses in a 52-year-old female who was previously treated for adenocarcinoma of the sigmoid colon. A histologic study of the surgical specimen from the sinonasal cavity demonstrated a tumour identical to the patient's prior primary tumour of the colon. The sinonasal neoplastic tissue showed marked positivity for carcinoembryonic antigen and expressed cytokeratin 20, which differentiates metastatic colonic adenocarcinoma from ITAC., Treatment/outcome: The patient received palliative radiation but died 3 months after the diagnosis., Conclusion: Distinguishing metastatic adenocarcinoma from gastrointestinal tract from ITAC can be difficult. In view of the resemblance, immunohistochemical staining can help in differentiating them. It is important to recognise these as metastatic lesions as the treatment is mainly palliative.
- Published
- 2008
38. Living bilayered human skin equivalent: promising potentials for wound healing.
- Author
-
Mazlyzam AL, Aminuddin BS, Saim L, and Ruszymah BH
- Subjects
- Antigens, Neoplasm, Biomarkers, Tumor, Cell Proliferation, Endopeptidases, Enzyme-Linked Immunosorbent Assay, Gelatinases, Humans, Membrane Proteins, Serine Endopeptidases, Fibroblast Growth Factor 2 physiology, Fibroblast Growth Factor 7 physiology, Fibroblasts physiology, Keratinocytes physiology, Neovascularization, Physiologic physiology, Transforming Growth Factor beta1 physiology, Vascular Endothelial Growth Factor A physiology, Wound Healing physiology
- Abstract
The angiogenic potential of native skin (NS), keratinocytes single skin equivalent (SSE-K), fibroblasts single skin equivalent (SSE-F) and bilayered skin equivalent secreting angiogenic growth factors such as transforming growth factor beta1 (TGF-beta1), vascular endothelial growth factor (VEGF), keratinocyte growth factor (KGF) and basic fibroblast growth factor (bFGF) in the in vitro systems at 24, 48, 72 hours and 7 days was compared using Enzyme-Linked Immunosorbent Assay (ELISA). Bilayered skin equivalent exhibit highest release of growth factors within 24 hours to 7 days of culture compared to NS, SSE-K and SSE-F. This proved the potential of bilayered skin equivalent in producing and sustaining growth factors release to enhance angiogenesis, fibroblasts proliferation, matrix deposition, migration and growth of keratinocytes.
- Published
- 2008
39. Lateral sinus thrombosis.
- Author
-
Nurliza I and Saim L
- Subjects
- Adult, Anti-Bacterial Agents administration & dosage, Anti-Bacterial Agents therapeutic use, Child, Humans, Lateral Sinus Thrombosis diagnosis, Lateral Sinus Thrombosis drug therapy, Lateral Sinus Thrombosis surgery, Malaysia, Male, Tomography, X-Ray Computed, Lateral Sinus Thrombosis pathology
- Abstract
We describe four cases of lateral sinus thrombosis secondary to otitis media. They presented with low-grade fever, headache, nausea, vomiting and ear discharge. One patient had facial nerve palsy. CT scan was helpful in managing these patients. They were treated with antibiotics followed by surgery. Two patients had intracranial abscesses and were treated accordingly.
- Published
- 2007
40. Rare bone disorder affecting the temporal bone.
- Author
-
Sreetharan SS, Hazim M, and Saim L
- Subjects
- Adolescent, Adult, Fibrous Dysplasia, Polyostotic physiopathology, Fibrous Dysplasia, Polyostotic surgery, Hearing Disorders surgery, Humans, Magnetic Resonance Imaging, Male, Temporal Bone surgery, Tomography, X-Ray Computed, Fibrous Dysplasia, Polyostotic diagnosis, Hearing Disorders etiology, Temporal Bone physiopathology
- Abstract
Fibrous dysplasia is an uncommon benign disorder of unknown etiology. Rarely, it presents isolated in the temporal bone. We present three cases of monostotic fibrous dysplasia that involved the entire temporal bone.
- Published
- 2006
41. Massive epistaxis secondary to pseudoaneurysm of internal carotid artery.
- Author
-
Asma A, Putra SH, and Saim L
- Subjects
- Adult, Carotid Artery Diseases complications, Epistaxis diagnosis, Humans, Male, Carotid Artery, Internal physiopathology, Carotid-Cavernous Sinus Fistula complications, Craniocerebral Trauma complications, Epistaxis etiology
- Abstract
Post-traumatic pseudoaneurysms of internal carotid arteries are uncommon. The patients may present with massive epistaxis due to rupture of the aneurysm into the sphenoid sinus. Early diagnosis and treatment is mandatory as the likelihood of exsanguinations increases with each subsequent episode of epistaxis. The clinical features of unilateral blindness and massive epistaxis after head injury should indicate the diagnosis. The high mortality of this entity underlines the importance of early angiography in these patients to confirm this diagnosis. We present 3 cases of post-traumatic aneurysm of the ICA.
- Published
- 2006
42. Newborn hearing screening: experience in a Malaysian hospital.
- Author
-
Abdullah A, Hazim MY, Almyzan A, Jamilah AG, Roslin S, Ann MT, Borhan L, Sani A, Saim L, and Boo NY
- Subjects
- Audiometry, Evoked Response, Evoked Potentials, Auditory, Brain Stem, False Positive Reactions, Follow-Up Studies, Hearing Loss congenital, Hearing Loss epidemiology, Humans, Infant, Infant, Newborn, Malaysia, Pilot Projects, Predictive Value of Tests, Prevalence, Hearing Loss diagnosis, Neonatal Screening, Otoacoustic Emissions, Spontaneous
- Abstract
Introduction: This study aims to determine the prevalence of hearing loss among newborns delivered at Hospital Universiti Kebangsaan Malaysia and to evaluate the usefulness of our hearing screening protocol., Methods: All infants born in the hospital over a nine-month period, between April to December 2003, were screened for hearing loss with a portable otoacoustic emission (OAE) before discharge. At the age of two months, a second OAE test was repeated on newborns who failed the initial test. Those who failed the second test were re-tested at three months of age. When these infants failed the third OAE test, a brainstem evoked response (BSER) test was performed., Results: During the study period, 4,219 infants were born in the hospital, and 3,762 (89.2 percent) underwent OAE screening. 620 (19.7 percent) of them failed the first screening test, and 506 (81.6 percent) of them came for a second stage-screening test. In the third stage screening at three months of age, only 39 (65 percent) patients turned up. Of these, ten infants passed the OAE test and 29 failed. However, when these infants underwent BSER, 13 had normal BSER and 16 have abnormal BSER. The prevalence of hearing loss in this study was 0.42 percent (16/3,762)., Conclusion: The large number of defaulters and false-positive results in this study suggest that this pilot hearing-screen programme requires fine-tuning to minimise these problems.
- Published
- 2006
43. Congenital deafness: high prevalence of a V37I mutation in the GJB2 gene among deaf school children in Alor Setar.
- Author
-
Ruszymah BH, Wahida IF, Zakinah Y, Zahari Z, Norazlinda MD, Saim L, and Aminuddin BS
- Subjects
- Adolescent, Child, Connexin 26, Deafness congenital, Female, Humans, Malaysia epidemiology, Male, Prevalence, Connexins genetics, Deafness epidemiology, Deafness genetics, Mutation, Missense
- Abstract
Twenty percent of all childhood deafness is due to mutations in the GJB2 gene (Connexin 26). The aim of our study was to determine the prevalence and spectrum of GJB2 mutations in childhood deafness in Malaysia. We analyzed the GJB2 gene in 51 deaf students from Sekolah Pendidikan Khas Alor Setar, Kedah. Bidirectional sequencing indicates that 25% of our childhood deafness has mutation in their GJB2 gene. Sixty two percent of these children demonstrate V37I missense mutation. Interestingly, V37I mutation in the GJB2 gene have been reported as polymorphism in Western countries, however in our country it behaved as a potentially disease-causing missense mutation, causing childhood deafness as it was not found in the normal control.
- Published
- 2005
44. Sensitivity and specificity of portable transient otoacoustic emission (TEOAE) in newborn hearing screening.
- Author
-
Abdullah A, Long CW, Saim L, and Mukari SZ
- Subjects
- Hearing Tests, Humans, Infant, Newborn, Malaysia, Sensitivity and Specificity, Cochlear Microphonic Potentials, Hearing Loss diagnosis, Neonatal Screening, Otoacoustic Emissions, Spontaneous
- Abstract
Early identification and management of hearing impairment is very valuable. The goal standard measurement of hearing loss is by brainstem evoked response (BSER). This prospective study was conducted in Hospital University Kebangsaan Malaysia (HUKM) to determine the sensitivity and specificity of transient evoked otoacoustic emission (TEOAE) as a screening tool for hearing impairment from February 1999 to February 2000. One hundred and thirty-three newborns from postnatal ward and seventy-eight newborns from neonatal intensive care unit (NICU) were screened for possible hearing loss using portable TEOAE. This study showed that TEOAE is a very sensitive but moderately specific screening tool.
- Published
- 2005
45. Management of iatrogenic facial nerve palsy and labyrinthine fistula in mastoid surgery.
- Author
-
Long YT, bin Sabir Husin Athar PP, Mahmud R, and Saim L
- Subjects
- Adult, Facial Paralysis surgery, Female, Fistula surgery, Humans, Labyrinth Diseases surgery, Male, Retrospective Studies, Facial Paralysis etiology, Fistula etiology, Iatrogenic Disease, Labyrinth Diseases etiology, Mastoid surgery
- Abstract
A 6-year review of complications of mastoid surgery between June 1995 and June 2001 revealed five cases with serious iatrogenic complications from mastoid surgery, of which four were facial nerve palsy and two were labyrinthine fistula. One of these patients had concomitant facial nerve palsy and labyrinthine fistula. There were two cases of complete facial nerve palsy (House Brackmann grade VI) and two cases of incomplete palsy (House Brackmann grades IV and V). The second genu was the site of injury in three of the four cases. Of the four cases with facial nerve palsy, two patients had full recovery (House Brackmann grade I), one recovered only to House Brackmann grade III, and one was lost to follow-up. Both patients with labyrinthine fistula had postoperative vertigo and profound sensorineural hearing loss. The site of iatrogenic fenestration was the lateral semicircular canal in both cases.
- Published
- 2004
- Full Text
- View/download PDF
46. Formation of tissue-engineered human auricular cartilage via tissue engineering technique for future use in ear surgery.
- Author
-
Saim L, Aminuddin BS, Munirah S, Chua KH, Izuddin Fahmy A, Fuzina NH, Isa MR, and Ruszymah BH
- Subjects
- Cell Count, Cell Division physiology, Cells, Cultured, Elastin metabolism, Humans, Microscopy, Cartilage transplantation, Chondrocytes cytology, Ear, External surgery, Tissue Engineering methods
- Abstract
To date there is no optimal approach to reconstruct an external ear. However, advances in tissue engineering technologies have indicated that in vitro autologous elastic cartilage might be of great importance in the future treatment of these patients. The aim of this study was to observe monolayer expansion of auricular cartilage and to evaluate engineered cartilage using standard histochemical study.
- Published
- 2004
47. Gene expression characteristic in human auricular cartilage tissue engineering.
- Author
-
Farah Wahida I, Aminuddin BS, Munirah S, Chua KH, Fuzina NH, Isa MR, Saim L, and Ruszymah BH
- Subjects
- Actins genetics, Animals, Cartilage transplantation, Cells, Cultured, Cellular Senescence physiology, Ear, External, Fibroblasts cytology, Gene Expression physiology, Humans, Mice, Mice, Nude, Reverse Transcriptase Polymerase Chain Reaction, Chondrocytes cytology, Collagen Type I genetics, Collagen Type II genetics, Phenotype, Tissue Engineering methods
- Abstract
This study was to assess collagen type II and collagen type I gene expression in tissue-engineered human auricular: cartilage formed via tissue engineering technique. Large-scale culture expansions were transformed into 3D in vitro construct and were implanted subcutaneously on the dorsal of athymic mice. After 8 weeks, explanted construct was processed in the same manner of native cartilage to facilitate cells for gene expression analysis. Isolated cells from in vivo construct demonstrated expression of type II collagen gene comparable to native cartilage. This study verified that tissue-engineered auricular cartilage expressed cartilage specific gene, collagen type II after in vivo maturation.
- Published
- 2004
48. Phenotypic expression of collagen type II and collagen type I gene in monolayer culture of human auricular chondrocytes.
- Author
-
Nur Adelina AN, Aminuddin BS, Munirah S, Chua KH, Fuzina NH, Saim L, and Ruszymah BH
- Subjects
- Cells, Cultured, Ear, External, Fibroblasts cytology, Humans, Reverse Transcriptase Polymerase Chain Reaction, Chondrocytes cytology, Collagen Type I genetics, Collagen Type II genetics, Phenotype, Tissue Engineering methods
- Abstract
Cartilage is regularly needed for reconstructive surgery. Basic research in tissue engineering is necessary to develop its full potential. We presented here the expression profile of type II collagen gene and type I collagen gene in human auricular monolayer culture expansion. Cultured chondrocytes documented a reduction in the expression level of collagen type II gene whilst collagen type I gene was gradually expressed through all the passages. This study demonstrated that human auricular chondrocytes lose its phenotypic expression during monolayer culture expansion. Further studies are required to enhance cartilage specific gene expression, collagen type II throughout the in vitro culture.
- Published
- 2004
49. The different faces of facial nerve schwannomas.
- Author
-
Abdullah A, Mahmud MR, Sabir HA, and Saim L
- Subjects
- Adult, Cranial Nerve Neoplasms complications, Facial Nerve Diseases complications, Female, Humans, Male, Middle Aged, Neurilemmoma complications, Cranial Nerve Neoplasms diagnosis, Facial Nerve Diseases diagnosis, Neurilemmoma diagnosis
- Abstract
Facial nerve schwannomas are rare benign tumors. The tumor can arise anywhere along the course of the facial nerve. The most common presentation for this tumor is a slowly progressive facial nerve paralysis. Sensorineural hearing loss (SNHL) and tinnitus are later symptoms. The symptoms and signs depend on the site of tumor along the nerve. We report three cases of facial nerve schwannomas with different clinical presentations. Appropriate management of a facial nerve schwannoma should be based on the site and extent of the tumor and status of the nerve function.
- Published
- 2003
50. Surgical intervention in traumatic facial nerve paralysis.
- Author
-
Yeoh TL, Mahmud R, and Saim L
- Subjects
- Adolescent, Adult, Humans, Male, Middle Aged, Facial Nerve Injuries complications, Facial Nerve Injuries surgery, Facial Paralysis etiology, Facial Paralysis surgery, Skull Fractures complications, Temporal Bone injuries
- Abstract
A four years review from June 1998 to June 2002 of traumatic facial nerve paralysis from temporal bone fractures that required surgical intervention is presented. The aim of this clinical presentation was to determine the current pattern of cases with traumatic facial paralysis which required surgical intervention at our center. There were six cases, of which four (66%) were longitudinal fractures, one each (17%) had transverse fracture and fracture over the lateral wall of mastoid. Hearing loss (83%) was the commonest associated clinical symptom. All cases underwent decompression via the transmastoid surgical approach. Intraoperative findings revealed oedema of facial nerve involving vertical segment and horizontal segment in three cases each respectively. Two cases had concomitant bony impingement. The facial nerve functions in four cases (66%) and one case recovered to House Brackmann grade 2 and 4, 12 months and 3 months respectively postsurgery. The case with transverse fracture remained as House Brackmann grade 5 after two years.
- Published
- 2003
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