1. Mammalian GW220/TNGW1 is essential for the formation of GW/P bodies containing miRISC
- Author
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Lee Spraggon, Saif Naseeruddin, Matthew Adamow, Virginia Castilla-Llorente, Sarah Qamar, Miwako Okamura, and Jidong Liu
- Subjects
RNA-induced silencing complex ,RNA Stability ,RNA-binding protein ,Biology ,Autoantigens ,Article ,RNA interference ,Cell Line, Tumor ,Gene expression ,microRNA ,P-bodies ,Gene silencing ,Animals ,Humans ,RNA-Induced Silencing Complex ,RNA, Messenger ,Cellular localization ,Research Articles ,RNA-Binding Proteins ,Cell Biology ,Molecular biology ,Cell biology ,MicroRNAs ,HEK293 Cells ,RNA Interference ,HeLa Cells - Abstract
Localization of the miRNA-induced silencing complex to GW/P bodies by GW220/TNGW1 may regulate the fate of target mRNAs., The microRNA (miRNA)-induced silencing complex (miRISC) controls gene expression by a posttranscriptional mechanism involving translational repression and/or promoting messenger RNA (mRNA) deadenylation and degradation. The GW182/TNRC6 (GW) family proteins are core components of the miRISC and are essential for miRNA function. We show that mammalian GW proteins have distinctive functions in the miRNA pathway, with GW220/TNGW1 being essential for the formation of GW/P bodies containing the miRISC. miRISC aggregation and formation of GW/P bodies sequestered and stabilized translationally repressed target mRNA. Depletion of GW220 led to the loss of GW/P bodies and destabilization of miRNA-targeted mRNA. These findings support a model in which the cellular localization of the miRISC regulates the fate of the target mRNA.
- Published
- 2012