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7. Study on structure and morphology of silicon-substituted LiFePO4/C doped material.

Author

Astuti, Fahmi, Maghfirohtuzzoimah, Vera L., Intifadhah, Sahara H., Az-Zahra, Pelangi, Ramadhan, Muhammad R., Zainuri, Mochamad, and Darminto, Darminto

Subjects
SCANNING transmission electron microscopy, DOPING agents (Chemistry), CARBON electrodes, ENERGY storage
Abstract

Elemental doping and carbon coating on the electrode are able to increase the battery's performance to be a good energy storage. In this case, some studies are necessary to confirm the quality of the cathode material, LiFePO4 on the Li-ion based battery material (LIB). Here we report the study of structure and morphology of LiFePO4 by silicon doping at the P-atom atomic position and carbon coating (henceforth called as LFP-Si) utilizing the X-Ray diffractometer, the Fourier-transform infrared technique, and both the scanning and transmission electron microscopy (SEM & TEM), as a preliminary investigation to know the structure and morphology of LiFePO4 after elemental doping and carbon coating. All characterization results are essential for the improvement of the performance of LiFePO4/C doped material. [ABSTRACT FROM AUTHOR]

Published
2023
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26. General Lectures

Author

Yamaoka, Y., Nagai, T., Furuta, K., Inagawa, T., Sugiya, T., Kai, T., Amamoto, H., Okunara, T., Miyoshi, A., Araya, S., Sometani, T., Ogura, T., Yamato, T., Hirata, S., Hashimoto, T., Hamanaka, Y., Shakudo, Y., Ozaki, T., Noda, S., Kobayashi, K., Sasaki, K., Matsuura, R., Ueno, H., Ito, T., Umayahara, A., Koga, Y., Watanabe, K., Nabeya, K., Shimura, I., Ohyama, O., Komatsuzaki, T., Ogoshi, K., Hara, Y., Hiratsuka, H., Kubo, N., Masuda, H., Inoue, S., Arakawa, H., Koizumi, K., Mukozima, K., Inoue, Y., Hosaka, H., Kikuchi, N., Yoshida, H., Sakumoto, I., Inaba, M., Yokoi, Y., Abei, T., Iwama, S., Shirota, A., Miki, M., Ōkawa, K., Onda, M., Yoshioka, M., Shiba, T., Yamashita, K., Moriyama, Y., Adachi, K., Miyashita, M., Henmi, H., Egami, K., Toi, K., Fukiwake, T., Ito, H., Tamesue, N., Ohsato, K., Nagamitsu, S., Nishimura, M., Yamashita, Y., Yao, T., Mizuno, S., Tanabe, M., Yanase, M., Suzuki, K., Suzuki, K., Hayashi, K., Nishitani, T., Katake, K., Iwasa, N., Nishimura, S., Miyoshi, M., Fukumoto, K., Fujii, H., Inatomi, I., Nakajima, H., Hojo, Y., Tosaka, T., Kaneko, H., Yoshikawa, K., Mori, K., Uematsu, T., Takahashi, T., Morikawa, S., Hashi, M., Sakamoto, T., Kimura, A., Sasagawa, T., Maeda, Y., Matsukawa, M., Aizawa, T., Tabata, I., Munakata, A., Toda, S., Tajima, T., Matsunaga, F., Ogata, T., Nakayama, K., Nakayama, T., Minota, S., Otani, A., Takei, S., Tanaka, M., Miki, H., Hojo, K., Hirota, E., Sano, R., Murashima, Y., Okuuti, Y., Miwa, K., Suga, T., Yaosaka, T., Namiki, M., Kawauti, H., Nakagawa, K., Kasukawa, T., Kobayashi, S., Watanabe, H., Yamagata, S., Narasaka, T., Imai, H., Tsuneoka, T., Watanabe, H., Hoshi, K., Nishiyama, S., Hoshi, K., Fushimi, I., Hirai, T., Katsuda, M., Hirose, M., Yokomori, H., Matsumoto, T., Watanabe, N., Matsuura, K., Ishibashi, T., Nakata, S., Takei, C., Asano, H., Miyoshi, H., Hidaka, T., Dodo, H., Kitada, A., Nakamura, T., Sakata, S., Kitamura, S., Nakamua, T., Sakata, S., Kitamura, S., Agata, E., Aikawa, K., Oshima, A., Fujimoto, I., Kobayashi, T., Asakawa, Y., Kusakari, M., Abe, C., Tarumi, S., Yamashita, T., Takasu, S., Komase, Y., Hamada, H., Shoji, F., Saito, S., Takayama, T., Fujita, R., Kumura, F., Umeda, K., Okamoto, S., Nishio, H., Shintani, Y., Saitoh, K., Tatara, T., Iwamiya, K., Tamura, M., Tamura, K., Nakano, A., Tamura, U., Nakajima, T., Ichioka, G., Takeuchi, Y., Ayada, K., Torisu, R., Kamada, H., Matuoka, R., Turuoka, M., Sagara, Y., Nakamura, S., Sakasita, O., Mashimo, N., Sekiguchi, T., Kobayashi, S., Kishimoto, H., Takeuchi, T., Murakami, S., Koga, S., Ueno, M., Nishizawa, M., Nomoto, K., Kariya, A., Hayashi, M., Kobayashi, S., Mizuno, K., Mayama, S., Shinozuka, T., Maruyama, T., Ogiwara, T., Okui, K., Higurashi, K., Ito, T., Miyata, Y., Tamura, T., Ikeda, S., Nakata, J., Oshima, H., Mori, S., Otsuka, Y., Oki, I., Tasaka, S., Yamahatsu, J., Inaba, E., Sanada, K., Oura, T., Kinoshita, T., Akagi, M., Katsuhisa, F., Misumi, A., Urashima, K., Ninomiya, S., Hukami, M., Mori, T., Matsuo, Y., Seki, A., Kitamura, T., Mori, H., Yokota, R., Kawashima, S., Itoshima, T., Shimada, Y., Itoshima, T., Inoue, T., Fukuhara, J., Kubota, M., Ohta, W., Ohta, W., Kagaya, T., Abe, R., Kai, Y., Katono, S., Komatsu, K., Masuda, H., Inoue, S., Arakawa, H., Hamajima, T., Kitamura, T., Nakagawa, F., Tamura, H., Kiyonaga, G., Inui, H., Asai, H., Hayashi, N., Obata, H., Toki, F., Kakae, U., Yamauchi, D., Hisamitsu, T., Aziki, K., Tamiya, M., Watanabe, S., Kurokawa, K., Takemoto, T., Murakami, S., Kessoku, Y., Kuwana, H., Hino, K., Kato, A., Ito, A., Arakawa, Y., Ohono, Y., Hase, M., Ariga, K., Usui, R., Kutsukake, S., Nagamori, S., Nagano, H., Shimano, K., Ohya, T., Kikuchi, S., Ito, M., Hidano, S., Banno, H., Tomura, A., Kato, K., Koyama, T., Komatsu, T., Takei, T., Tomimura, K., Yamauchi, M., Sato, G., Sato, R., Haga, M., Toyokawa, S., Yamamoto, J., Ohtomi, S., Ishibashi, Y., Fukuda, M., Endo, R., Ueno, Y., Hisamitsu, T., Sasaki, T., Kobayashi, C., Kusakari, T., Yajima, T., Maeda, M., Kotoda, K., Okuda, K., Ariga, H., Takazawa, G., Nakamura, Y., Ohbayashi, A., Mitsui, H., Nakata, K., Suematsu, T., Kashiwagi, T., Hayashi, N., Baba, T., Tobimatsu, Y., Kamada, T., Abe, H., Matsuoka, K., Matsushima, S., Kamisaka, Y., Kitsuki, T., Ohnuki, H., Fujii, M., Inoue, R., Yamamoto, T., Wakisaka, G., Nakagawa, S., Nagata, K., Takebayashi, J., Nagashima, H., Tanaka, N., Kanai, K., Oda, T., Katayama, T., Furukawa, Y., Miyasaki, R., Noguchi, M., Hirose, K., Maezawa, H., Kano, H., Hirano, K., Ogino, M., Nishiwaki, K., Aoki, T., Morishita, T., Funatsu, K., Morita, A., Okazaki, I., Matsuzaki, S., Oda, M., Asakura, H., Kamegaya, K., Tsuchiya, M., Sambe, K., Kawakami, H., Kunimasa, T., Aimitsu, A., Yamashita, S., Miyoshi, A., Enzan, H., Ikehara, K., Shiozaki, Y., Sameshima, Y., Mizuno, T., Sasakawa, M., Nagi, S., Nagata, T., Fuwa, H., Tatsumi, K., Komatsu, K., Ozeki, T., Kaneda, M., Otsuki, M., Tadaki, H., Miura, K., Yamagata, S., Iwamura, K., Yamanaka, I., Sugimoto, E., Yamazaki, Y., Shiraishi, I., Yamanaka, T., Koike, H., Shimura, S., Hirayama, Y., Nishikawa, H., Kawamura, T., Kamiyama, Y., Takeda, H., Kamano, Y., Kitamura, O., Yamaoka, Y., Nanbu, H., Ozawa, K., Takasan, H., Honjo, I., Itakura, H., Akanuma, Y., Kagaya, T., Kaito, I., Sato, S., Sahara, H., Arisue, T., Kashimura, K., Motoyama, W., Hayashi, H., Okuyama, S., Ito, S., Inagaki, T., Kato, Y., Kakumu, S., Kurokawa, S., Yamawaki, T., Kusakabe, A., Hara, T., Funayama, A., Takahashi, T., Furuta, S., Omori, A., Hanaoka, S., Nagata, A., Tsukioka, J., Kiyosawa, K., Akahane, Y., Koike, Y., Oda, M., Tanaka, K., Kojima, M., Kawaguchi, Y., Kimura, A., Osamura, H., Kurihara, N., Okabe, K., Fujisawa, K., Takahashi, T., Kitami, N., Namihisa, T., Yamaguchi, K., Hisauchi, T., Nambu, M., Iijima, K., Rin, K., Kuroda, H., Kobayashi, N., Inami, Y., Shiga, K., Kon, T., Yamada, T., Yamada, T., Mizoguchi, Y., Enomoto, T., Monna, T., Yamamoto, S., Morisawa, S., Imoto, S., Uchita, K., Yamasawa, Y., Hiraide, S., Hikita, G., Takatsuki, K., Okimoto, Y., Nakagawa, J., Ito, K., Hirayama, C., Kawasaki, H., Irisa, T., Arimura, K., Amagase, H., Shibasaki, K., Tashiro, S., Ichida, F., Tozawa, T., Ishii, M., Inoue, E., Ikehara, H., Baba, S., Miyaji, Y., Nakajima, K., Shimizu, T., Shimizu, Y., Ohnishi, S., Sasaki, S., Kinami, Y., Mizukami, T., Nishida, Y., Nakagawa, T., Ojima, T., Takeshita, Y., Yamashita, T., Furuto, T., Ono, T., Yamaguchi, K., Mizuno, S., Tsumori, K., Miyagi, K., Suga, Y., Tatsumi, S., Kitano, A., Makiishi, H., Mitani, E., Mohri, S., Kamata, T., Kobayashi, K., Yamamoto, S., Yoshii, T., Takemoto, T., Suzuki, H., Hiratsuka, H., Takada, K., Maruyama, M., Takemoto, T., Suzuki, H., Katsu, K., Nomura, M., Kiyama, T., Hirabayashi, H., Yamashita, H., Masuyama, S., Takehara, Y., Sato, T., Abe, H., Sugiura, M., Shima, F., Ichihara, S., Yamasaki, Z., Fukuzawa, S., Horiguchi, Y., Takeda, T., Nakano, S., Kitamura, K., Miwa, M., Suzuke, T., Okada, K., Nakamura, T., Kikuchi, T., Mishima, K., Mandai, M., Kondo, H., Yamagata, Y., Uchida, Y., Harada, H., Nishizawa, M., Nomoto, K., Kariya, A., Ueno, M., Hayashi, M., Kobayashi, S., Mizuno, K., Shinozuka, T., Maruyama, T., Ogiwara, T., Okui, K., Miyake, N., Okada, M., Takahashi, K., Koizumi, H., Hayashi, T., Maeda, H., Abe, M., Takahashi, I., Matsumoto, M., Unoura, T., Iwasaki, A., Hattori, T., Tanaka, M., Hara, T., Sato, H., Hirashima, T., Shioda, A., Kawamura, I., Muto, M., Tsuchiya, R., Sato, Y., Ozawa, T., Hatano, T., Arae, H., Sekine, T., Tsukamoto, M., Shiratori, T., Asaki, S., Oba, E., Yamagata, H., Kobiyama, M., Hisamichi, S., Kitagawa, M., Kobayashi, N., Kurosawa, T., Tokimatsu, S., Kawasaki, S., Iwasa, A., Nagashima, K., Kodeki, K., Hoshizawa, T., Murakami, H., Yagi, T., Matsuda, T., Iwazaki, T., Suzuki, Y., Taketomi, H., Akaike, Y., Naramoto, J., Tsuru, T., Inoue, M., Nagase, T., Kato, K., and Kohyama, K.

Published
1973
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27. Effect of Aging on the Progression of Pulmonary Ischemia-Reperfusion Injury in Clawn Miniature Swine.

Author

Sahara, H., Ichinari, Y., Iwanaga, T., Sekijima, M., Takeuchi, K., and Shimizu, A.

Subjects
*RADIOGRAPHIC films, *AGE differences, *CHEST X rays, *SWINE, *LYMPHOID tissue, *REPERFUSION injury, *LYMPHATIC metastasis
Abstract

Transplantation using aged donor organs is reported to have a poor prognosis. We hypothesized that inducible bronchus-associated lymphoid tissue (iBALT) is more likely to be induced in aged donor lungs, thus promoting lung damage and rejection. In this study, we evaluated the relationship between iBALT induction and lung injury during pulmonary ischemia-reperfusion injury using young and aged CLAWN miniature swine. Warm ischemia was induced for 90 minutes by clamping the left pulmonary artery and veins. Lung functions were serially accessed via blood gas analysis, chest x-ray films (CXRs), and lung biopsies. Pigs older than 3 years were used as the aging group (n=4), whereas pigs around 6 months old were used as the young group (n=5). Serum cytokine levels (TNF-α, IL-6, HMGB1) and oxidative stress were measured to characterize the inflammatory response to pulmonary IRI. Although direct assessment of impaired lung using left PV blood showed a slightly worse P/F ratio in the aging group (210 ± 69 vs. 292 ± 33 mmHg at 2 days), there were no significant differences in preoperative data between two groups, including the degree of lung injuries based on arterial and left PV blood gas, CXRs. Histological scores calculated by cellular infiltration, intra-alveolar edema, fibrin exudation, and hemorrhage, no significant differences were observed between the two groups Limited iBALT induction was only observed in the aging groups. Furthermore, serum levels of proinflammatory antioxidant cytokines and showed slightly higher levels in the aging group though there was no significant differences between two groups. In this model, age difference alone did not affect the progress of lung injuries, including iBALT induction. These results indicated that activation of innate immune system alone is not sufficient for the progression of pulmonary IRI and induction of iBALT. Future studies will be conducted with lung transplant models, taking into account the involvement of cold ischemia, acquired immune system, or lymphangiogenesis/angiogenesis. [ABSTRACT FROM AUTHOR]

Published
2023
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30. Thymectomy Does Not Abrogate Long-Term Acceptance of MHC Class I-Disparate Lung Allografts

Author

Sahara, H., Weiss, M.J., Ng, C.Y., Houser, S.L., Pujara, A.C., Sayre, J.K., Wain, J.C., Sachs, D.H., Madsen, J.C., and Allan., J.S.

Subjects
Graft Rejection, Genotype, Swine, Histocompatibility Antigens Class I, Homozygote, Animals, Swine, Miniature, Transplantation, Homologous, Thymectomy, Article, Immunosuppressive Agents, Tacrolimus, Lung Transplantation
Abstract

We have previously reported that tolerance to class I disparate lung allografts in miniature swine could be induced using an intensive 12-day course of tacrolimus and that pretransplant sensitization with immunogenic MHC class I allopeptides failed to block the induction of tolerance. We also have previously reported the importance of the presence of the thymus in the induction of tolerance to isolated heart, kidney, and combined heart-kidney transplants. In this study, we examined the impact of thymectomy on tolerance induction in lung transplantation.Orthotopic left lung transplantation was performed using MHC class I-disparate donors. The recipients received a 12-day course of high-dose tacrolimus (n = 6). Total thymectomies were performed in three of the swine 21 days prior to transplantation. Lung grafts were monitored by chest radiography and serial open lung biopsy.All euthymic recipients maintained their grafts for over 1 year. None of the thymectomized recipients has experienced graft loss in the 6 to 10 months following transplantation. Although isolated lesions of obliterative bronchiolitis were occasionally seen in one thymectomized animal on biopsy, donor-specific unresponsiveness has been observed on assays of cell-mediated lymphocytotoxicity in all recipients. Moreover, co-culture assays have shown that recipient lymphocytes can strongly inhibit the normally robust response of naïve recipient-matched lymphocytes to donor antigen. This inhibition was not seen when using stimulators primed with third-party antigens against appropriate targets.These data suggest that thymus-independent peripheral regulatory mechanisms may be sufficient to induce and maintain long-term acceptance of the lung allografts.

Published
2006

36. Radioresistance of cancer stem-like cell derived from canine tumours.

Author

Tanabe, A., Deguchi, T., Sato, T., Nemoto, Y., Maruo, T., Madarame, H., Shida, T., Naya, Y., Ogihara, K., and Sahara, H.

Subjects
LUNG cancer treatment, CANIDAE, CANCER radiotherapy, CANCER stem cells, DRUG resistance in cancer cells, DISEASES
Abstract

Cancer stem-like cells ( CSCs)/cancer-initiating cells ( CICs) are a small subpopulation of cancer cells that are responsible for the initiation, recurrence and metastasis of cancer. We previously demonstrated that, using the Hoechst 33342 dye-based side population technique, CSCs/ CICs in canine lung adenocarcinoma cell line exist. In this study, as CSCs/ CICs are known to form spheres in anchorage-independent environment in vitro, we evaluated the stemness of spheroid cells derived from canine lung adenocarcinoma and osteosarcoma cells by expression of stemness markers, and investigated radioresistance. Spheroid cells showed greater expression of stemness markers Oct-4 and CD133 gene than those of adherent-cultured cells. In nude mouse xenograft models, spheroid cells showed higher tumourigenic ability than adherent-cultured cells. In addition, spheroid cells showed significantly resistant against radioactivity as compared with adherent-cultured cells. These results suggest that spheroid cells could possess stemness and provide a CSCs/ CICs research tool to investigate CSCs/ CICs of canine tumour cells. [ABSTRACT FROM AUTHOR]

Published
2016
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