88 results on '"Saha BP"'
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2. Oriental medicine mangifera indica
- Author
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Rai, Sujay, primary, Basak, Souvik, additional, Mukherjee, Kakali, additional, Saha, BP, additional, and Mukherjee, Pulok K, additional
- Published
- 2007
- Full Text
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3. Diuretic activity of extract of the rhizomes of nelumbo nucifera gaertn. (Fam. Nymphaeaceae)
- Author
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PULOK K MUKHERJEE, Pal, M., Saha, K., Saha, Bp, and Das, J.
4. Cytisus scoparius link--a natural antioxidant.
- Author
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Sundararajan R, Haja NA, Venkatesan K, Mukherjee K, Saha BP, Bandyopadhyay A, and Mukherjee PK
- Abstract
BACKGROUND: Recent investigations have shown that the antioxidant properties of plants could be correlated with oxidative stress defense and different human diseases. In this respect flavonoids and other polyphenolic compounds have gained the greatest attention. The plant Cytisus scoparius contains the main constituent of flavone and flavonals. The present study was undertaken to evaluate the in vitro antioxidant activities of extract of aerial part of Cytisus scoparius. METHODS: The plant extract was tested for DPPH (1, 1-diphenyl, 2-picryl hydrazyl) radical scavenging, nitric oxide radical scavenging, superoxide anion radical scavenging, hydroxyl radical scavenging, antilipid peroxidation assay, reducing power and total phenol content. RESULTS: The extract exhibited scavenging potential with IC50 value of 1.5 microg/ml, 116.0 microg/ml and 4.7 microg/ml for DPPH, nitric oxide and superoxide anion radicals. The values were found to lesser than those of vitamin C, rutin, and curcumin, as standards. The extract showed 50% protection at the dose of 104.0 microg/ml in lipid peroxidation induced by Fe2+/ ascorbate system in rat liver microsomal preparation. There is decrease in hydroxyl radical generation with IC50 value of 27.0 microg/ml when compared with standard vitamin E. The reducing power of the extract depends on the amount of extract. A significant amount of polyphenols could be detected by the equivalent to 0.0589 microg of pyrocatechol from 1 mg of extract. CONCLUSION: The results obtained in the present study indicate that hydro alcoholic extract of aerial part of Cytisus scoparius is a potential source of natural antioxidants. [ABSTRACT FROM AUTHOR]
- Published
- 2006
5. Amitriptyline-induced ventricular tachycardia: a case report.
- Author
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Sabah KMN, Chowdhury AW, Islam MS, Saha BP, Kabir SR, and Kawser S
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- Adult, Female, Humans, Tachycardia, Ventricular drug therapy, Amitriptyline poisoning, Antidepressive Agents, Tricyclic poisoning, Suicide, Attempted, Tachycardia, Ventricular chemically induced, Tachycardia, Ventricular physiopathology
- Abstract
Background: In Bangladesh, each emergency physician faces amitriptyline overdose nearly a day. An acute cardiovascular complication, one of the worst complications is mainly responsible for the mortality in tricyclic overdose. Recently, we managed ventricular tachycardia in a young female presented with an impaired consciousness 10 h after intentionally ingesting 2500 mg amitriptyline. Here, we report it, discuss how the electrocardiography is vital to acknowledge and predict it and its' complications and also the recent update of the management of it., Case Presentation: A young married Bangladeshi-Bengali girl, 25-year-old, having a history of disharmony with her husband, came with an impaired consciousness after intentionally ingesting 2500 mg amitriptyline about 10 h before arrival. There was blood pressure 140/80 mmHg, heart rate 140 beats-per-min, temperature 103 °F, Glasgow coma scale 10/15, wide complex tachycardia with QRS duration of 178 ms in electrocardiography, blood pH 7.36. Initially, treated with 100 ml 8.4% sodium bicarbonate. After that, QRS duration came to 100 ms in electrocardiography within 10 min of infusion. To maintain the pH 7.50-7.55 over the next 24 h, the infusion of 8.4% sodium bicarbonate consisting of 125 ml dissolved in 375 ml normal saline was started and titrated according to the arterial blood gas analysis. Hence, a total dose of 600 mmol sodium bicarbonate was given over next 24 h. In addition to this, gave a 500 ml intravenous lipid emulsion over 2 h after 24 h of admission as she did not regain her consciousness completely. Afterward, she became conscious, though, in electrocardiography, ST/T wave abnormality persisted. So that, we tapered sodium bicarbonate infusion slowly and stopped it later. At the time of discharge, she was by heart rate 124/min, QRS duration 90 ms in electrocardiogram along with other normal vital signs., Conclusion: Diagnosis of amitriptyline-induced ventricular tachycardia is difficult when there is no history of an overdose obtained. Nevertheless, it should be performed in the clinical background and classic electrocardiographic changes and wise utilization of sodium bicarbonate, intravenous lipid emulsion, and anti-arrhythmic drugs may save a life.
- Published
- 2017
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6. Soya phospholipid complex of mangiferin enhances its hepatoprotectivity by improving its bioavailability and pharmacokinetics.
- Author
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Bhattacharyya S, Ahmmed SM, Saha BP, and Mukherjee PK
- Subjects
- Animals, Antioxidants administration & dosage, Antioxidants chemistry, Antioxidants metabolism, Antioxidants therapeutic use, Biomarkers blood, Carbon Tetrachloride Poisoning blood, Carbon Tetrachloride Poisoning metabolism, Carbon Tetrachloride Poisoning physiopathology, Carbon Tetrachloride Poisoning prevention & control, Half-Life, Hepatic Insufficiency etiology, Kinetics, Liver metabolism, Liver physiopathology, Male, Nutritive Value, Oxidative Stress, Particle Size, Phospholipids chemistry, Protective Agents chemistry, Protective Agents metabolism, Protective Agents therapeutic use, Rats, Wistar, Seeds chemistry, Xanthones chemistry, Xanthones metabolism, Xanthones therapeutic use, Dietary Fats metabolism, Dietary Supplements analysis, Hepatic Insufficiency prevention & control, Phospholipids metabolism, Protective Agents administration & dosage, Glycine max chemistry, Xanthones administration & dosage
- Abstract
Background: Mangiferin is a xanthonoid present in Mangifera indica. It has been reported for a variety of pharmacological activities, including hepatoprotection. However, the major disadvantage of mangiferin is its reduced biological activity due to poor absorption, low bioavailability and rapid elimination from the body after administration. The aim of this study was to prepare a phospholipid complex of mangiferin to overcome these limitations and to investigate the impact of the complex on hepatoprotective activity and bioavailability., Results: The results showed that the complex has an enhanced hepatoprotective and in vivo antioxidant activity as compared to pure mangiferin at the same dose level (30 and 60 mg kg⁻¹). The complex restored the levels of serum hepatic marker enzymes and liver antioxidant enzymes with respect to carbon tetrachloride-treated animals. The complex also increased the bioavailability of mangiferin in rat serum by 9.75-fold compared to pure mangiferin at the same dose level and enhanced the elimination half-life (t(1/2 el)) from 1.71 ± 0.12 h⁻¹ to 3.52 ± 0.27 h⁻¹., Conclusion: The results suggested that the complexation of mangiferin with soya phospholipid enhanced the hepatoprotection and in vivo antioxidant activity, which may be due to the improved bioavailability and pharmacokinetics of mangiferin in rat serum., (© 2013 Society of Chemical Industry.)
- Published
- 2014
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7. Impact of MDA and the prospects of elimination of the lone focus of diurnally sub periodic lymphatic filariasis in Nicobar Islands, India.
- Author
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Shriram AN, Krishnamoorthy K, Sivan A, Saha BP, Kumaraswami V, and Vijayachari P
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- Adolescent, Adult, Aedes growth & development, Aged, Aged, 80 and over, Animals, Child, Child, Preschool, Cross-Sectional Studies, Demography, Drug Therapy methods, Elephantiasis, Filarial prevention & control, Elephantiasis, Filarial transmission, Female, Humans, India epidemiology, Infant, Infant, Newborn, Insect Vectors, Islands epidemiology, Male, Middle Aged, Prevalence, Young Adult, Anthelmintics therapeutic use, Disease Eradication methods, Elephantiasis, Filarial drug therapy, Elephantiasis, Filarial epidemiology
- Abstract
Mass Drug Administration is being carried out in Andaman and Nicobar Islands, India since 2004. Cross sectional microfilaria (Mf) survey was conducted in Nancowry group of islands, the lone foci of diurnally sub periodic form of bancroftian filariasis in Nicobar district, to examine its eligibility for Transmission Assessment Survey (TAS). A total of 2561 individuals (coverage: 23.9%) were screened from five islands. The overall Mf prevalence was 3.28%. Except one island, all other islands recorded Mf prevalence >1%, ranging from 2.5% to 5.3%, indicating persistence of infection despite six annual rounds of MDA. Mf prevalence was age dependent and was higher among males, but not significantly different between genders. Age and gender specific analysis showed a significant reduction in all the age classes among females vis a vis pre-MDA prevalence while the reduction was significant only in 21-30 and 41-50 age classes in males. Exposure to day biting and forest dwelling Downsiomyia nivea can be attributed for the persistent infection besides non-compliance for MDA. Based on fits of modified negative binomial distribution, true prevalence of Mf carriers in the community was estimated to be 4.74%, which is markedly higher (about 24%) than the observed prevalence of 3.28%. Follow up of cohorts showed evidence of continued persistence of infection and acquisition of new infections post six rounds of MDA. As the Mf prevalence was above >1% in four of the five islands, this area is not eligible for TAS, warranting continuation of MDA. Mass DEC fortified salt is suggested as an adjunct to hasten elimination of infection., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2014
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8. Chlorogenic acid-phospholipid complex improve protection against UVA induced oxidative stress.
- Author
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Bhattacharyya S, Majhi S, Saha BP, and Mukherjee PK
- Subjects
- Animals, Catalase metabolism, Glutathione Peroxidase metabolism, In Vitro Techniques, Male, Oxidative Stress drug effects, Rats, Rats, Wistar, Skin metabolism, Skin radiation effects, Skin Absorption, Superoxide Dismutase metabolism, Thiobarbituric Acid Reactive Substances metabolism, Chlorogenic Acid pharmacology, Phospholipids pharmacology, Radiation-Protective Agents pharmacology, Skin drug effects, Ultraviolet Rays adverse effects
- Abstract
The mammalian skin is prone to oxidative damage when exposed to ultraviolet (UV) rays from the sun light. The antioxidants like chlorogenic acid (CA) can protect the skin from the ill effects of UV radiation when applied topically. But conventional topical formulations of these phytomolecules could not protect the skin for long duration owing to their rapid distribution in the systemic circulation. Therefore the aim of the present investigation was to prepare a novel topical formulation of CA which can exert its protective effect for long time after its application. The phospholipid complex of the CA was prepared and evaluated against oxidative stress produced in the rat skin due to UVA exposure. Compared to the conventional formulation, the complex exerted improved protection when UVA irradiation was performed after 4h of topical application. Thus the results ascertain the superiority of CA-phospholipid complex over conventional formulation in terms of protection against UVA radiation for long duration., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2014
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9. The gallic acid-phospholipid complex improved the antioxidant potential of gallic acid by enhancing its bioavailability.
- Author
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Bhattacharyya S, Ahammed SM, Saha BP, and Mukherjee PK
- Subjects
- Animals, Antioxidants pharmacokinetics, Biological Availability, Chromatography, High Pressure Liquid, Gallic Acid pharmacokinetics, Gallic Acid pharmacology, Liver drug effects, Liver enzymology, Male, Microscopy, Electron, Scanning, Phospholipids pharmacokinetics, Phospholipids pharmacology, Rats, Rats, Wistar, Spectroscopy, Fourier Transform Infrared, Antioxidants pharmacology, Gallic Acid chemistry, Phospholipids chemistry
- Abstract
Gallic acid (GA) is well known for its antioxidant and hepatoprotective activity, though its effectiveness is restricted due to rapid metabolism and elimination. To overcome these problems, gallic acid-phospholipid complex was prepared and the effect of phospholipid complexation was investigated on carbon tetrachloride (CCl4)-induced oxidative damage in rat liver. The complex significantly reduced the hepatic marker enzymes in rat serum and restored the antioxidant enzyme levels with respect to CCl4-induced group (P < 0.05 and P < 0.01). Also, the complex improved the pharmacokinetics of GA by increasing the relative bioavailability and elimination half-life. The study therefore suggests that phospholipid complexation has enhanced the therapeutic efficacy of GA which may be due to its improved absorption and increased bioavailability in rat serum.
- Published
- 2013
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10. Lymphatic filariasis elimination programme in Andaman and Nicobar Islands, India: drug coverage and compliance post eight rounds of MDA.
- Author
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Sunish IP, Shriram AN, Sivan A, Kartick C, Saha BP, and Vijayachari P
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- Adult, Animals, Elephantiasis, Filarial prevention & control, Humans, India, Wuchereria bancrofti, Albendazole administration & dosage, Elephantiasis, Filarial drug therapy, Filaricides administration & dosage, Medication Adherence statistics & numerical data
- Abstract
Lymphatic filariasis (LF) is endemic in the Andaman and Nicobar islands, including the lone foci for a diurnally sub-periodic form of Wuchereria bancrofti in the Nancowry group of islands. A programme to eliminate LF was launched in 2004 by the Directorate of Health Services, Andaman and Nicobar Administration which involved a single annual mass drug administration (MDA) using diethylcarbamazine (DEC) with albendazole. So far, eight rounds of MDA have been implemented through the Public Health Care network. The pattern of antifilarial drug distribution and compliance achieved in the on-going LF elimination programme in these islands has been assessed. This is the first systematic effort undertaken in these remote islands to assess the coverage and compliance with the LF elimination programme. This study covered 900 households in each of the 3 districts. There were a largest number of side effects in the Nicobar district (6.4%). Non-consumption of drugs ranged from 18.6% (Nicobar) to 42% (North and Middle Andaman). A survey revealed that almost 95.3% of the respondents had heard about MDA from the drug distributors. Therefore, the distributors should be involved in programmes designed to educate the community at risk of acquiring filarial infection and the possible side effects of the drugs.
- Published
- 2013
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11. Diurnally subperiodic filariasis in India-prospects of elimination: precept to action?
- Author
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Shriram AN, Krishnamoorthy K, Saha BP, Roy A, Kumaraswami V, Shah WA, Jambulingam P, and Vijayachari P
- Subjects
- Animals, Culicidae parasitology, Humans, India epidemiology, Diet Therapy methods, Dietary Supplements, Diethylcarbamazine administration & dosage, Filariasis epidemiology, Filariasis prevention & control, Filaricides administration & dosage, Wuchereria bancrofti isolation & purification
- Abstract
The elimination of lymphatic filariasis in the Andaman and Nicobar Islands provides unique opportunities and challenges at the same time. Since these islands are remote, are sparsely populated, and have poor transport networks, mass drug administration programs are likely to be difficult to implement. Diurnally subperiodic Wuchereria bancrofti vectored by Downsiomyia nivea was considered for the scope of vector control options. Considering the bioecology of this mosquito, vector control including personal protection measures may not be feasible. However, since these islands are covered by separate administrative machinery which also plays an important role in regulating the food supply, the use of diethylcarbamazine (DEC)-fortified salt as a tool for the interruption of transmission is appealing. DEC-fortified salt has been successfully pilot tested in India and elsewhere, operationally used by China for eliminating lymphatic filariasis. Administration of DEC-fortified salt though simple, rapid, safe, and cost-effective, challenges are to be tackled for translating this precept into action by evolving operationally feasible strategy. Although the use of DEC-fortified salt is conceptually simple, it requires commitment of all sections of the society, an elaborate distribution mechanism that ensures the use of DEC-fortified salt only in the endemic communities, and a vigorous monitoring mechanism. Here, we examine the inbuilt administrative mechanisms to serve the tribal people, health infrastructure, and public distribution system and discuss the prospects of putting in place an operationally feasible strategy for its elimination.
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- 2011
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12. Analgesic, anti-inflammatory, antipyretic and toxicological evaluation of some newer 3-methyl pyrazolone derivatives.
- Author
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Mariappan G, Saha BP, Sutharson L, Singh A, Garg S, Pandey L, and Kumar D
- Abstract
In this paper, we described the pharmacological and toxicological studies of three pyrazolone derivatives namely PYZ1: 4-[4-N dimethylamino benzylidine]-3-methyl pyrazolin-5(4H)-one, PYZ2: 4-[2-chlorobenzylidine]-3-methylpyrazolin-5(4H)-one and PYZ3: 4-[benzylidine]-3-methylpyrazolin-5(4H)-one derivatives. Analgesic, anti-inflammatory and antipyretic studies of 3-methyl pyrazolone derivatives at 400 mg/kg, p.o. have shown significant activity as compared to control. Amongst three pyrazolone derivatives, PYZ2 was found to be more active. Based on the result of pharmacological studies, PYZ2 was selected for toxicological studies. Acute toxicity studies revealed that methyl pyrazolone derivatives are non-toxic in rats up to 5000 mg/kg, p.o. The subacute toxicity study of PYZ2 showed that decrease in Hb content, RBC and WBC count. In biochemical analysis level of blood glucose and bilirubin reduced where as AST, ALT and alkaline phosphatase level elevated. Histopathological studies revealed that there was mild toxicity on liver and kidney at 1000 mg/kg, p.o.
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- 2011
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13. Exploring the potential of Nelumbo nucifera rhizome on membrane stabilization, mast cell protection, nitric oxide synthesis, and expression of costimulatory molecules.
- Author
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Mukherjee D, Biswas A, Bhadra S, Pichairajan V, Biswas T, Saha BP, and Mukherjee PK
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- Animals, B7-1 Antigen analysis, B7-2 Antigen analysis, CD40 Antigens analysis, Cell Degranulation drug effects, Erythrocytes drug effects, Histamine analysis, Lipopolysaccharides antagonists & inhibitors, Lipopolysaccharides pharmacology, Male, Mice, Mice, Inbred C57BL, Nitric Oxide analysis, Nitrites analysis, Pentacyclic Triterpenes, Rats, Rats, Wistar, Triterpenes pharmacology, Betulinic Acid, Cytoprotection, Erythrocyte Membrane drug effects, Macrophages drug effects, Mast Cells drug effects, Nelumbo, Nitric Oxide biosynthesis, Plant Extracts pharmacology, Rhizome
- Abstract
Immunomodulatory activity of Nelumbo nucifera rhizome was evaluated for its standardized extract (NNRE) with respect to betulinic acid. Various key parameters including erythrocyte membrane stabilization, inhibition of histamine release, reduction in nitric oxide production and depletion of expression of costimulatory molecules of macrophages were estimated. The result displayed that NNRE stabilized erythrocyte membrane significantly at 10 (42.05%) and 100 microg/mL (44.31%). Although considering the protection of mast cells from degranulation, NNRE showed 38.66% (100 microg/mL) and 69.66% (10 microg/mL) degranulation against compound 48/80 (C 48/80). NNRE at 1 and 5 microg/mL inhibited lipopolysaccharide (LPS)-induced activation of macrophages by decreasing the expression of costimulatory molecules. Expression of CD40, CD80, and CD86 by NNRE was seen significantly at 5 microg/mL compared to LPS-treated group. The extracts also inhibited the nitrite concentration at 1 and 5 microg/mL compared to LPS-treated group.
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- 2010
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14. Acetylcholinesterase inhibitory potential of a carbazole alkaloid, mahanimbine, from Murraya koenigii.
- Author
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Kumar NS, Mukherjee PK, Bhadra S, Saha BP, and Pal BC
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- Carbazoles chemistry, Carbazoles isolation & purification, Chromatography, Thin Layer, Plant Leaves chemistry, Carbazoles pharmacology, Cholinesterase Inhibitors isolation & purification, Murraya chemistry
- Abstract
In the search for acetylcholinesterase (AChE) inhibitors from Indian medicinal plants, via bioassay-guided isolation, a carbazole alkaloid, mahanimbine [3, 5-dimethyl-3-(4- methylpent-3-enyl)-11H-pyrano [5, 6-a] carbazole], was isolated from the petroleum ether extract of the leaves of Murraya koenigii. Inhibition of AChE was evaluated based on Ellman's method using 96-well microplate readers. Mahanimbine inhibited AChE activity in a dose-dependent manner with an IC(50) value of 0.03 +/- 0.09 mg/mL, while galantamine was used as a standard. The AChE inhibitory activity of this carbazole alkaloid has not been reported so far, and this study is the first to reveal this activity in carbazole alkaloid mahanimbine, isolated from Murraya koenigii., (Copyright (c) 2009 John Wiley & Sons, Ltd.)
- Published
- 2010
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15. Evaluation of antioxidant potential of pyrazolone derivatives.
- Author
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Mariappan G, Saha BP, Bhuyan NR, Bharti PR, and Kumar D
- Abstract
In this article the antioxidant property of pyrazolones derivatives (PYZ1 to PYZ10) are reported. It was assessed by estimation of Malonaldehyde (MDA) and 4-Hydroxyl-2-noneal (4-HNE) as lipid peroxidation markers in myocardial ischemic reperfusion injury. The inhibition of lipid peroxidation was compared with the standard ascorbic acid. Among synthesized derivatives PYZ2, PYZ3, PYZ7, PYZ8, PYZ9, and PYZ10 were found to have potent antioxidant effect against MDA marker. In case of 4-HNE, PYZ4, PYZ5, PYZ6, PYZ7, PYZ8, PYZ9 and PYZ10 were found to have effective antioxidant activity and the rest of the compounds are moderately active. Comparatively PYZ7, PYZ8, PYZ9 and PYZ10 are having effective role to control both MDA and 4-HNE generation. All the experimental data were statistically significant at p< 0.05 level. Interestingly, beyond its NSAID property, this study explores the protective role of pyrazolone derivatives in ischemic heart injury.
- Published
- 2010
16. Immunomodulatory potential of rhizome and seed extracts of Nelumbo nucifera Gaertn.
- Author
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Mukherjee D, Khatua TN, Venkatesh P, Saha BP, and Mukherjee PK
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- Animals, Erythrocytes drug effects, Female, Fever drug therapy, Hypersensitivity, Delayed drug therapy, Immunologic Factors administration & dosage, Inflammation drug therapy, Male, Mice, Neutrophils drug effects, Sheep, Hypersensitivity, Delayed immunology, Immunologic Factors pharmacology, Nelumbo chemistry, Rhizome chemistry, Seeds chemistry
- Abstract
Ethnopharmacological Relevance: Nelumbo nucifera Gaertn. (Nymphaeaceae) is a well-known aquatic plant which has been used for the treatment of several disorders including skin disease, cough, inflammation, fever and many other disorders., Aim of the Study: To explore the immunomodulatory activity of extract of rhizome (NNRE) and seed (NNSE) of the plant., Materials and Methods: The immunomodulatory activity of NNRE and NNSE was evaluated using various in vivo models including the total and differential leukocyte count (TLC and DLC), nitroblue-tetrazolium reduction (NBT) test, neutrophil adhesion test, phagocytic response and delayed type hypersensitivity (DTH) reaction. Sheep red blood cells (SRBC, 5 x 10(9)cells/ml) were used to immunize the animals. NNRE and NNSE at the doses of 100 and 300 mg/kg were administrated., Result: The TLC and lymphocyte count increased significantly but the neutrophil count was decreased for NNRE and NNSE treated groups compared to the control. A dose-dependent potentiation of DTH reaction induced by SRBC was observed from the extracts. The percentage of neutrophil adhesion to the nylon fiber was increased in NNRE treated groups (63.22 and 62.91%) compared to the NNSE treated group (54.86 and 54.23%). A potential phagocytic response was seen on treatment of the extracts, and significant changes were observed in the formation of formazone crystals., Conclusion: This finding suggests that the extract of rhizome and seed Nelumbo nucifera stimulate defense system by modulating several immunological parameters., (Copyright (c) 2010 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2010
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17. Acetylcholinesterase enzyme inhibitory potential of standardized extract of Trigonella foenum graecum L and its constituents.
- Author
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Satheeshkumar N, Mukherjee PK, Bhadra S, and Saha BP
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- Alkaloids analysis, Cholinesterase Inhibitors analysis, Chromatography, High Pressure Liquid, Galantamine pharmacology, Inhibitory Concentration 50, Plant Extracts chemistry, Reference Standards, Seeds, Alkaloids pharmacology, Cholinesterase Inhibitors pharmacology, Plant Extracts pharmacology, Trigonella chemistry
- Abstract
Ethno pharmacological approach has provided several leads to identify potential new drugs from plant sources, including those for memory disorders. Acetylcholinesterase inhibitors (AChEI) give a symptomatic relief to some of the clinical manifestations of the disease. The main objective of this study is to standardize the extract of Trigonella foenum graecum L with trigonelline by HPTLC method and determine the in vitro AChE inhibitory activity of Trigonella foenum graecum L and its constituents using galanthamine as a reference. Different concentrations of hydro alcoholic extract of Trigonella foenum graecum and trigonelline were subjected to HPTLC analysis using the mobile phase n propanol, methanol and water (4:1:2, v/v). The R(f) of trigonelline was found to be 0.43, and the correlation coefficient of 0.99 was indicative of good linear dependence of peak area on concentration. The concentration of trigonelline was found to be 13mgg(-1)w/w in the hydro alcoholic extract of Trigonella foenum graecum. The AChE inhibitory activity of crude fenugreek seed extracts, fractions and trigonelline was evaluated using Ellman's method in 96-well micro plate's assay and TLC bioassay detection. The ethyl acetate fraction of the alcohol extract (IC50 53.00 +/- 17.33microg/ml), and total alkaloid fraction (IC50 9.23+/-6.08microg/ml) showed potential AChE inhibition. Trigonelline showed IC50 233+/-0.12microM. Galanthamine was used as standard and it showed inhibition of acetyl cholinesterase with an IC50 value of 1.27+/-0.21microM., (Copyright 2009 Elsevier GmbH. All rights reserved.)
- Published
- 2010
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18. Enhancing bioavailability and hepatoprotective activity of andrographolide from Andrographis paniculata, a well-known medicinal food, through its herbosome.
- Author
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Maiti K, Mukherjee K, Murugan V, Saha BP, and Mukherjee PK
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- Animals, Anti-Inflammatory Agents pharmacology, Anti-Inflammatory Agents therapeutic use, Biological Availability, Carbon Tetrachloride, Carbon Tetrachloride Poisoning blood, Carbon Tetrachloride Poisoning enzymology, Chemical and Drug Induced Liver Injury blood, Chemical and Drug Induced Liver Injury enzymology, Diterpenes pharmacology, Diterpenes therapeutic use, Liposomes pharmacology, Liposomes therapeutic use, Liver enzymology, Male, Phospholipids, Plant Extracts pharmacology, Plant Extracts therapeutic use, Rats, Rats, Wistar, Andrographis chemistry, Anti-Inflammatory Agents administration & dosage, Carbon Tetrachloride Poisoning drug therapy, Chemical and Drug Induced Liver Injury drug therapy, Diterpenes administration & dosage, Liver drug effects, Plant Extracts administration & dosage
- Abstract
Background: Andrographis paniculata is a health food used extensively in Southeast Asia, India and China and contains the pharmacologically important phytochemical andrographolide. Although andrographolide has antihepatotoxic activity, its bioavailability from A. paniculata is restricted by its rapid clearance and high plasma protein binding. The aim of this study was to formulate a herbosome of andrographolide with a naturally occurring phospholipid in order to enhance the bioavailability and hepatoprotective activity of andrographolide in rats., Results: Andrographolide herbosome equivalent to 25 and 50 mg kg(-1) andrographolide significantly protected the liver of rats, restoring hepatic enzyme activities with respect to carbon tetrachloride-treated animals (P < 0.05 and P < 0.01 respectively). The rat plasma concentration of andrographolide obtained from the complex equivalent to 25 mg kg(-1) andrographolide (C(max) = 9.64 microg mL(-1)) was higher than that obtained from 25 mg kg(-1) andrographolide (C(max) = 6.79 microg mL(-1)), and the complex maintained its effective plasma concentration for a longer period of time., Conclusion: The results proved that the andrographolide complex produced by this method has better bioavailability and hence improved hepatoprotective activity compared with andrographolide at the same dose. Andrographolide complexation is therefore helpful in solving the problem of rapid clearance and low elimination half-life associated with andrographolide from A. paniculata., (Copyright (c) 2009 Society of Chemical Industry.)
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- 2010
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19. Exploring the effect of Hesperetin-HSPC complex--a novel drug delivery system on the in vitro release, therapeutic efficacy and pharmacokinetics.
- Author
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Maiti K, Mukherjee K, Murugan V, Saha BP, and Mukherjee PK
- Subjects
- Animals, Antioxidants pharmacokinetics, Antioxidants therapeutic use, Calibration, Carbon Tetrachloride Poisoning drug therapy, Carbon Tetrachloride Poisoning metabolism, Chemistry, Pharmaceutical, Drug Delivery Systems, Hesperidin pharmacokinetics, Hesperidin therapeutic use, Liver Function Tests, Male, Rats, Rats, Wistar, Reference Standards, Reproducibility of Results, Solubility, Glycine max chemistry, Suspensions, Antioxidants chemistry, Hesperidin chemistry, Phosphatidylcholines chemistry
- Abstract
Hesperetin is known to exhibit a variety of pharmacological activities in mammalian cell systems. Although it shows appreciable bioavailability when administered orally, its faster elimination from body creates the need of frequent administration to maintain effective plasma concentration. To overcome this limitation, a phospholipid complex of hesperetin was prepared and evaluated for antioxidant activity and pharmacokinetic profile. The hesperetin content of the complex was determined by a spectrophotometer and the surface characteristics of the complex were studied by means of microscope. The antioxidant activity was evaluated in carbon-tetrachloride-intoxicated rats at a dose level of 100 mg/kg body weight, p.o. The complex was studied for in vitro drug release characteristics and effect of complexation on serum concentration of hesperetin in rats was also studied along with main pharmacokinetic parameters. The results showed that the complex has a sustained release property and enhanced antioxidant activity (P < 0.05 and <0.01) as compared to free hesperetin at the same dose level. Pharmacokinetic study depicted that the complex has higher relative bioavailability and acted for a longer period of time. The study therefore suggests that phospholipid complex of hesperetin produced better antioxidant activity than free drug at the same dose level and the effect persisted for a longer period of time, which may be helpful in solving the problems of faster elimination of the molecule.
- Published
- 2009
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20. Curcumin-phospholipid complex: Preparation, therapeutic evaluation and pharmacokinetic study in rats.
- Author
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Maiti K, Mukherjee K, Gantait A, Saha BP, and Mukherjee PK
- Subjects
- Animals, Area Under Curve, Calorimetry, Differential Scanning, Carbon Tetrachloride Poisoning metabolism, Catalase metabolism, Chemical and Drug Induced Liver Injury metabolism, Curcumin administration & dosage, Curcumin chemistry, Glutathione metabolism, Glutathione Peroxidase metabolism, Glutathione Reductase metabolism, Glutathione Transferase metabolism, Half-Life, Male, Phosphatidylcholines chemistry, Rats, Rats, Wistar, Solubility, Superoxide Dismutase metabolism, Thiobarbituric Acid Reactive Substances metabolism, Carbon Tetrachloride Poisoning drug therapy, Chemical and Drug Induced Liver Injury drug therapy, Curcumin pharmacokinetics, Phosphatidylcholines pharmacology
- Abstract
A novel formulation of curcumin in combination with the phospholipids was developed to overcome the limitation of absorption and to investigate the protective effect of curcumin-phospholipid complex on carbon tetrachloride induced acute liver damage in rats. The antioxidant activity of curcumin-phospholipid complex (equivalent of curcumin 100 and 200 mg/kg body weight) and free curcumin (100 and 200 mg/kg body weight) was evaluated by measuring various enzymes in oxidative stress condition. Curcumin-phospholipid complex significantly protected the liver by restoring the enzyme levels of liver glutathione system and that of superoxide dismutase, catalase and thiobarbituric acid reactive substances with respect to carbon tetrachloride treated group (P < 0.05 and <0.01). The complex provided better protection to rat liver than free curcumin at same doses. Serum concentration of curcumin obtained from the complex (equivalent to 1.0 g/kg of curcumin) was higher (Cmax 1.2 microg/ml) than pure curcumin (1.0 g/kg) (Cmax 0.5 microg/ml) and the complex maintained effective concentration of curcumin for a longer period of time in rat serum. The result proved that curcumin-phospholipid complex has better hepatoprotective activity, owe to its superior antioxidant property, than free curcumin at the same dose level.
- Published
- 2007
- Full Text
- View/download PDF
21. Determination of 6-gingerol in ginger (Zingiber officinale) using high-performance thin-layer chromatography.
- Author
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Rai S, Mukherjee K, Mal M, Wahile A, Saha BP, and Mukherjee PK
- Subjects
- Catechols, Chromatography, Thin Layer statistics & numerical data, Food Analysis methods, Food Analysis statistics & numerical data, Chromatography, Thin Layer methods, Fatty Alcohols analysis, Zingiber officinale chemistry
- Abstract
A sensitive and accurate High-Performance TLC (HPTLC) method has been developed to determine the quantity of 6-gingerol in rhizomes of Zingiber officinale (family: Zingiberaceae), commonly known as ginger. Methanol extracts of rhizomes from three different sources were used for HPTLC, n-hexane, and diethyl ether (40:60 v/v) as the mobile phase. The Rf of 6-gingerol was found to be 0.40. The calibration plot was linear in the range of 250-1200 ng of 6-gingerol and the correlation coefficient of 0.9997 was indicative of good linear dependence of peak area on concentration. The mean quantity of 6-gingerol was found to be 60.44+/-2.53 mg/g of ginger extract. The method permits reliable quantification of 6-gingerol and good resolution and separation of 6-gingerol from other constituents of ginger. To study the accuracy and precision of the method, recovery studies were performed by the method of standard addition. Recovery values from 99.79 to 99.84% showed the excellent reliability and reproducibility of the method. The proposed HPTLC method for quantitative monitoring of 6-gingerol in ginger can be used for routine quality testing of ginger extracts.
- Published
- 2006
- Full Text
- View/download PDF
22. Enhanced therapeutic potential of naringenin-phospholipid complex in rats.
- Author
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Maiti K, Mukherjee K, Gantait A, Saha BP, and Mukherjee PK
- Subjects
- Animals, Antioxidants chemistry, Antioxidants pharmacokinetics, Antioxidants therapeutic use, Carbon Tetrachloride, Catalase metabolism, Chemical and Drug Induced Liver Injury, Chemistry, Pharmaceutical, Delayed-Action Preparations, Flavanones blood, Flavanones chemistry, Flavanones pharmacokinetics, Flavanones therapeutic use, Glutathione Peroxidase metabolism, Lipid Peroxidation drug effects, Liver drug effects, Liver metabolism, Liver Diseases metabolism, Liver Function Tests, Male, Phospholipids chemistry, Phospholipids pharmacokinetics, Phospholipids therapeutic use, Rats, Rats, Wistar, Superoxide Dismutase metabolism, Antioxidants pharmacology, Flavanones pharmacology, Liver Diseases prevention & control, Phospholipids pharmacology
- Abstract
Naringenin is a naturally occurring flavanone, possessing a variety of biological activity. Due to its rapid elimination, naringenin needs frequent administration to maintain an effective plasma concentration. We have evaluated the therapeutic potential of naringenin-phospholipid complex under oxidative stress conditions compared with free naringenin. Naringenin-phospholipid complex was prepared and assessed for antioxidant activity in carbon tetrachloride intoxicated rats at a dose level of 100 mg kg-1 (p.o.). Liver function tests were studied by assessing serum glutamate oxaloacetate transaminase, serum glutamate pyruvate transaminase, serum alkaline phosphatase and total bilirubin. Marker enzymes of liver, namely glutathione peroxidase, superoxide dismutase, catalase and thiobarbituric acid reactive substances, were measured to evaluate the antioxidant potential at the same dose level. The plasma concentration of naringenin was also measured. It was observed that the naringenin-phospholipid complex enhanced the antioxidant activity of the biomolecule and protected the liver significantly for a longer time as compared with free naringenin at the same dose level. Phospholipid complex of naringenin produced better antioxidant activity than the free compound with a prolonged duration of action, which may be helpful in reducing the fast elimination of the molecule from body.
- Published
- 2006
- Full Text
- View/download PDF
23. Antioxidant activity of Nelumbo nucifera (sacred lotus) seeds.
- Author
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Rai S, Wahile A, Mukherjee K, Saha BP, and Mukherjee PK
- Subjects
- Animals, Biphenyl Compounds chemistry, Carbon Tetrachloride Poisoning drug therapy, Dose-Response Relationship, Drug, Free Radical Scavengers toxicity, Hydrazines chemistry, In Vitro Techniques, Kidney metabolism, Liver metabolism, Male, Mice, Nitric Oxide chemistry, Phytotherapy, Picrates, Plant Extracts chemistry, Plant Extracts pharmacology, Plant Extracts toxicity, Rats, Rats, Wistar, Rutin chemistry, Seeds, Superoxide Dismutase pharmacology, Thiobarbituric Acid Reactive Substances metabolism, Toxicity Tests, Acute, Free Radical Scavengers chemistry, Free Radical Scavengers pharmacology, Nelumbo
- Abstract
Antioxidant activity of hydro alcoholic extract of Nelumbo nucifera seeds (HANN) was studied using in vitro and in vivo models. Total phenolic content in HANN was found to be 7.61 +/- 0.04% (w/w). Characteristic HPTLC fingerprints of HANN were also made using different solvent systems. The HANN exhibited strong free radical scavenging activity as evidenced by the low IC(50) values in both DPPH (1,1-diphenyl-2-picryl hydrazyl) (6.12 +/- 0.41 microg/ml) and nitric oxide (84.86 +/- 3.56 microg/ml) methods. The values were found to be less than those of rutin, the standard used. Acute toxicity of HANN was evaluated in Swiss Albino mice, no signs of toxicity were observed up to the oral dose of 1,000 mg/kg body weight. Administration of HANN to Wistar rats at 100 and 200 mg/kg body weight for 4 days prior to carbon tetrachloride (CCl(4)) treatment caused a significant dose dependent increase (p < 0.05 to p < 0.001) in the level of superoxide dismutase (SOD) and catalase and a significant decrease (p < 0.05 to p < 0.001) in the level of thiobarbituric acid reactive substances (TBARS), when compared to CCl(4) treated control in both liver and kidney. These changes observed at 100 mg/kg body weight treatment were comparable to those observed for standard Vitamin E at 50 mg/kg treatment. Nelumbo nucifera seeds contain alkaloids, saponins, phenolics and carbohydrates. The results support significant antioxidant nature of HANN.
- Published
- 2006
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24. Antiinflammatory, analgesic and antiperoxidative efficacy of Barleria lupulina Lindl. extract.
- Author
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Suba V, Murugesan T, Kumaravelrajan R, Mandal SC, and Saha BP
- Subjects
- Analgesics chemistry, Analgesics therapeutic use, Animals, Anti-Inflammatory Agents chemistry, Anti-Inflammatory Agents therapeutic use, Antioxidants chemistry, Antioxidants therapeutic use, Carbon Tetrachloride, Dose-Response Relationship, Drug, Edema drug therapy, Edema pathology, Granuloma drug therapy, Granuloma pathology, Microsomes, Liver drug effects, Plant Components, Aerial chemistry, Plant Extracts chemistry, Rats, Rats, Wistar, Ulcer drug therapy, Ulcer pathology, Acanthaceae chemistry, Analgesics pharmacology, Anti-Inflammatory Agents pharmacology, Antioxidants pharmacology, Lipid Peroxidation drug effects, Plant Extracts pharmacology
- Abstract
A study was undertaken to investigate the antiinflammatory efficacy of methanol extract of aerial parts of Barleria lupulina Lindl. [MEBL] (Family: Acanthaceae) in acute and sub-acute inflammation models of albino rats. The MEBL in all the tested dose levels exhibited significant inhibition of carrageenin and serotonin induced paw oedema volumes when compared with the untreated (control) group. The MEBL also exerted a significant reduction in granuloma weight in the cotton pellet induced granuloma model. These potentials were comparable to that of the standard drug (indomethacin). The plant extract was also tested for its analgesic, ulcerogenic and antiperoxidative potential. The extract also demonstrated protection against CCl(4) induced lipid peroxidation and acetic acid induced writhing. Acute administration of MEBL (300 mg/kg) did not produce any gastric lesion in rats.
- Published
- 2005
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25. Antiulcer activity of methanol fraction of Barleria lupulina Lindl. in animal models.
- Author
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Suba V, Murugesan T, Pal M, Mandal SC, and Saha BP
- Subjects
- Animals, Anti-Ulcer Agents administration & dosage, Anti-Ulcer Agents therapeutic use, Duodenal Ulcer chemically induced, Ethanol, Female, Gastric Mucosa metabolism, Indomethacin, Lipid Peroxidation drug effects, Male, Plant Components, Aerial, Plant Extracts administration & dosage, Plant Extracts therapeutic use, Rats, Stomach drug effects, Stomach Ulcer chemically induced, Thiobarbituric Acid Reactive Substances, Acanthaceae, Anti-Ulcer Agents pharmacology, Duodenal Ulcer drug therapy, Phytotherapy, Plant Extracts pharmacology, Stomach Ulcer drug therapy
- Abstract
The objective of the present study was to evaluate the gastric cytoprotective activity of the methanol extract of aerial parts of the plant Barleria lupulina Lindl (Acanthaceae) in albino rats using various models of ulcers such as drug induced ulcers, restraint ulcers, duodenal ulcers and pylorus ligated ulcers. The effect of the extract on gastric secretion and lipid peroxidation (thiobarbituric acid reacting substances TBARS) was also studied in rats. The extract at the tested dose of 200 mg/kg significantly reduced the volume of gastric juice, total acidity and the ulcer index in pylorus ligated rats. It also afforded significant protection against alcohol and indomethacin induced ulcer as well as stress induced ulceration. TBARS in the stomach of indomethacin treated rats was also reduced. In addition, it gave protection against duodenal ulcers. The study suggests that the methanol extract of aerial parts of Barleria lupulina Lindl. has a protective effect against experimental gastric and duodenal ulcers., (Copyright 2004 John Wiley & Sons, Ltd.)
- Published
- 2004
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26. Evaluation of antitussive activity of Lagerstroemia parviflora leaf extract.
- Author
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Mazumder A, Saha BP, Basu SP, Mazumder R, Boominathan R, Devi BP, and Mandal SC
- Subjects
- Animals, Antitussive Agents administration & dosage, Antitussive Agents therapeutic use, Cough chemically induced, Dose-Response Relationship, Drug, Mice, Plant Extracts administration & dosage, Plant Extracts therapeutic use, Plant Leaves, Sulfur Dioxide, Antitussive Agents pharmacology, Cough prevention & control, Lagerstroemia, Phytotherapy, Plant Extracts pharmacology
- Abstract
The methanol extract of the leaves of Lagerstroemia parviflora Roxb was investigated for its effect on a cough model induced by sulfur dioxide gas in mice. It exhibited significant antitussive activity when compared with the control in a dose-dependent manner. The L. parviflora extract (100, 200, 300 mg/kg) showed maximum inhibition of cough reflex at 90 min after drug administration and the antitussive activity was comparable to that of codeine phosphate, a standard antitussive agent., (Copyright (c) 2004 John Wiley & Sons, Ltd.)
- Published
- 2004
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27. Anti-diabetic potential of Barleria lupulina extract in rats.
- Author
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Suba V, Murugesan T, Arunachalam G, Mandal SC, and Saha BP
- Subjects
- Animals, Blood Glucose drug effects, Diabetes Mellitus, Experimental chemically induced, Dose-Response Relationship, Drug, Hypoglycemic Agents administration & dosage, Hypoglycemic Agents therapeutic use, Male, Plant Components, Aerial, Plant Extracts administration & dosage, Plant Extracts therapeutic use, Rats, Rats, Wistar, Streptozocin, Acanthaceae, Diabetes Mellitus, Experimental prevention & control, Hypoglycemic Agents pharmacology, Phytotherapy, Plant Extracts pharmacology
- Abstract
We have undertaken a study to evaluate the anti-hyperglycemic effect of a methanol extract of aerial parts of Barleria lupulina Lindl. (Acanthaceae) in streptozotocin-diabetic rats, based on folkloric reports its use as an anti-diabetic agent. The extract exerted significant (p < 0.05) anti-hyperglycemic efficacy at all levels tested from 4 h after its administration, as compared with the control group, and the effect was also prolonged up to 12 h. The extract at doses of 200 mg kg(-1) body wt. and above exhibited a maximum activity (p < 0.001) at 12 h after administration. The most significant activity (15.35% blood glucose reduction) was observed for the group administered 300 mg kg(-1) body wt. at 12 h after administration, while the standard drug glibenclamide (10 mg/kg(-1) body wt.) showed an 18.80% reduction of blood glucose at the same time interval. Based on our current results, it appears that the methanol extract of aerial parts of Barleria lupulina Lindl. shows a pronounced blood-glucose-lowering potential in streptozotocin hyperglycemic rats, and is thus provided with a pharmacological support of the folklore claims of anti-diabetic activity.
- Published
- 2004
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28. Antidiabetic potential of Barleria lupulina extract in rats.
- Author
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Suba V, Murugesan T, Rao RB, Ghosh L, Pal M, Mandal SC, and Saha BP
- Subjects
- Administration, Oral, Animals, Diabetes Mellitus, Experimental drug therapy, Dose-Response Relationship, Drug, Hyperglycemia chemically induced, Hypoglycemic Agents administration & dosage, Hypoglycemic Agents therapeutic use, Male, Plant Components, Aerial, Plant Extracts administration & dosage, Plant Extracts therapeutic use, Rats, Rats, Wistar, Streptozocin, Acanthaceae, Hyperglycemia prevention & control, Hypoglycemic Agents pharmacology, Phytotherapy, Plant Extracts pharmacology
- Abstract
The methanol extract of aerial parts of Barleria lupulina orally tested at doses of 100, 200 and 300 mg/kg exerted significant antihyperglycemic effect in streptozotocin-induced hyperglycemia in rats [correction].
- Published
- 2004
- Full Text
- View/download PDF
29. Antitussive potential of methanol extract of stem bark of Ficus racemosa Linn.
- Author
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Bhaskara Rao R, Murugesan T, Pal M, Saha BP, and Mandal SC
- Subjects
- Administration, Oral, Animals, Antitussive Agents administration & dosage, Antitussive Agents therapeutic use, Cough chemically induced, Dose-Response Relationship, Drug, Female, Male, Mice, Plant Bark, Plant Extracts administration & dosage, Plant Extracts therapeutic use, Sulfur Dioxide, Antitussive Agents pharmacology, Cough prevention & control, Ficus, Phytotherapy, Plant Extracts pharmacology
- Abstract
The methanol extract of Ficus racemosa Linn (Moraceae) (stem bark) (MEFR) was tested for its antitussive potential against a cough induced model by sulphur dioxide gas in mice. The extract demonstrated significant (p < 0.001) antitussive activity at all tested dose levels when compared with the control. The antitussive activity of the extract was comparable to that of codeine phosphate (10 mg), a standard antitussive agent. The extract exhibited maximum inhibition of 56.9% at a dose of 200 mg/kg (p.o.) 90 min after administration., (Copyright 2003 John Wiley & Sons, Ltd.)
- Published
- 2003
- Full Text
- View/download PDF
30. Evaluation of purgative activity of roots of Rumex nepalensis.
- Author
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Ghosh L, Gayen JR, Murugesan T, Sinha S, Pal M, and Saha BP
- Subjects
- Administration, Oral, Animals, Cathartics administration & dosage, Cathartics therapeutic use, Defecation drug effects, Dose-Response Relationship, Drug, Female, Male, Plant Extracts administration & dosage, Plant Extracts therapeutic use, Plant Roots, Rats, Rats, Wistar, Cathartics pharmacology, Gastrointestinal Motility drug effects, Phytotherapy, Plant Extracts pharmacology, Rumex
- Abstract
The methanol extract of Rumex nepalensis Spreng. roots at the oral dose of 100-400 mg/kg exhibited significant and dose-dependent purgative activity.
- Published
- 2003
- Full Text
- View/download PDF
31. Antibacterial efficacy of Rumex nepalensis Spreng. roots.
- Author
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Ghosh L, Gayen JR, Sinha S, Pal S, Pal M, and Saha BP
- Subjects
- Anti-Bacterial Agents administration & dosage, Bacillus subtilis drug effects, Escherichia coli drug effects, Microbial Sensitivity Tests, Plant Extracts administration & dosage, Shigella dysenteriae drug effects, Staphylococcus aureus drug effects, Vibrio cholerae drug effects, Anti-Bacterial Agents pharmacology, Bacteria drug effects, Plant Extracts pharmacology, Rumex
- Abstract
The antibacterial property of Rumex nepalensis Spreng. was evaluated against some strains of bacteria. The methanol extract of the roots (tested at 200-1000 micro g/disc) showed significant concentration-dependent antibacterial activity., (Copyright 2003 John Wiley & Sons, Ltd.)
- Published
- 2003
- Full Text
- View/download PDF
32. Evaluation of wound healing activity of some herbal formulations.
- Author
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Mukherjee PK, Mukherjee K, Rajesh Kumar M, Pal M, and Saha BP
- Subjects
- Administration, Cutaneous, Animals, Anti-Infective Agents, Local administration & dosage, Anti-Infective Agents, Local pharmacology, Female, Male, Nitrofurazone administration & dosage, Nitrofurazone pharmacology, Nitrofurazone therapeutic use, Ointments, Plant Bark, Plant Extracts administration & dosage, Plant Extracts pharmacology, Rats, Rats, Wistar, Tensile Strength, Wound Healing drug effects, Wounds, Penetrating pathology, Anti-Infective Agents, Local therapeutic use, Phytotherapy, Plant Extracts therapeutic use, Skin drug effects, Skin injuries, Terminalia, Wounds, Penetrating drug therapy
- Abstract
The wound healing activity of two herbal formulations (Himax ointment and lotion) containing Indradaru extract, i.e. Arjuna bark (Terminalia arjuna, Family-Combretaceae), extract was evaluated for its wound healing potential in two types of wound models in rats (i) excision wound model and (ii) incision wound model. Both the formulations responded significantly in both the wound models tested. The results were also comparable to that of the standard drug nitrofurazone used as a standard drug for comparison in this present investigation. The results were also comparable in terms of wound contracting ability, epithelization period, tensile strength and regeneration of tissues at the wound area. Thus, this investigation con fi rms the use of the Himax ointment and lotion containing Terminalia arjuna extract as a wound-healing agent as known from folklore medicine., (Copyright 2003 John Wiley & Sons, Ltd.)
- Published
- 2003
- Full Text
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33. Psychopharmacological profiles of Leucas Lavandulaefolia Rees.
- Author
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Mukherjee K, Saha BP, and Mukherjee PK
- Subjects
- Animals, Anti-Anxiety Agents administration & dosage, Anti-Anxiety Agents therapeutic use, Dose-Response Relationship, Drug, Female, Male, Maze Learning drug effects, Mice, Motor Activity drug effects, Neuromuscular Agents administration & dosage, Neuromuscular Agents therapeutic use, Pentobarbital, Plant Extracts administration & dosage, Plant Extracts therapeutic use, Rats, Sleep drug effects, Anti-Anxiety Agents pharmacology, Behavior, Animal drug effects, Lamiaceae, Neuromuscular Agents pharmacology, Phytotherapy, Plant Extracts pharmacology
- Abstract
Methanol extract of Leucas lavandulaefolia (LLFE) was evaluated for different psychopharmacological profiles with various animal models in rats and mice. The extract showed a potential reduction in spontaneous activity and caused a significant decrease in exploratory behavioural pattern by the head dip and Y-maze test. It also showed a significant reduction in muscle relaxant activity by rotarod, 30 degrees inclined screen and traction tests. The extract showed a remarkable potentiation of pentobarbitone induced sleeping time in mice., (Copyright 2002 John Wiley & Sons, Ltd.)
- Published
- 2002
- Full Text
- View/download PDF
34. Evaluation of antipyretic potential of Leucas lavandulaefolia (Labiatae) aerial part extract.
- Author
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Mukherjee K, Saha BP, and Mukherjee PK
- Subjects
- Analgesics, Non-Narcotic administration & dosage, Analgesics, Non-Narcotic therapeutic use, Animals, Body Temperature drug effects, Dose-Response Relationship, Drug, Female, Fever chemically induced, Male, Plant Extracts administration & dosage, Plant Extracts therapeutic use, Plant Stems, Rats, Yeasts, Analgesics, Non-Narcotic pharmacology, Fever drug therapy, Lamiaceae, Phytotherapy, Plant Extracts pharmacology
- Abstract
Methanol extract of Leucas lavandulaefolia (LLFE) was subjected to antipyretic evaluation with yeast-induced pyrexia in rats. A yeast suspension (10 mL/kg, s.c.) increased the rectal temperature 19 h after administration. The extract at doses of 100, 200, 400 mg/kg (i.p.) produced significant dose dependent lowering of body temperature in yeast-provoked elevation of body temperature in rats. The antipyretic effect produced was comparable to that of a standard antipyretic drug, paracetamol., (Copyright 2002 John Wiley & Sons, Ltd.)
- Published
- 2002
- Full Text
- View/download PDF
35. Phytochemical observation on leaf of lagerstromia parviflora (roxb).
- Author
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Mazumber A, Basu SP, Saha BP, and Mazumder R
- Abstract
Phytochemical studies of leaf of the plant Lagerstroemia Parviflora Roxb (Lythraceae) reveals the presence of phytosterols, tannins, alkaloids, glycosides and absence of saponin, flavanoid and triterpenoids have been reported in this plant for the first time.
- Published
- 2002
36. Glucose lowering efficacy of Ficus racemosa bark extract in normal and alloxan diabetic rats.
- Author
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Bhaskara Rao R, Murugesan T, Sinha S, Saha BP, Pal M, and Mandal SC
- Subjects
- Animals, Diabetes Mellitus, Experimental blood, Diabetes Mellitus, Experimental mortality, Glyburide pharmacology, Hypoglycemic Agents pharmacology, Lethal Dose 50, Plant Bark chemistry, Rats, Rats, Wistar, Survival Rate, Toxicity Tests, Blood Glucose drug effects, Diabetes Mellitus, Experimental drug therapy, Ficus, Plant Extracts pharmacology
- Abstract
The glucose-lowering efficacy of a methanol extract of the stem bark of Ficus racemosa Linn. (MEBFR) (Family Moraceae) was evaluated both in normal and alloxan-induced diabetic rats. The MEBFR at the doses examined (200 and 400 mg/kg p.o.) exhibited significant hypoglycaemic activity in both experimental animal models when compared with the control group. The activity was also comparable to that of the effect produced by a standard antidiabetic agent, glibenclamide 10 mg/kg. The present investigation established pharmacological evidence to support the folklore claim that it is an antidiabetic agent., (Copyright 2002 John Wiley & Sons, Ltd.)
- Published
- 2002
- Full Text
- View/download PDF
37. The role of laser radiation therapy in maxillary sinusitis.
- Author
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Isser DK, Sett S, and Saha BP
- Abstract
Efficacy of prescribed noninvasive & invasive types of treatment of maxillary sinusitis has been compared with low-dose LASER therapy (LLT). After going through the observations of different authors on the therapeutic role of LLT (GaA1AS-LASER) in non-ENT infective diseases, its use in 'sinusitis' has been adjudged. Such type of study-report has not been found by us in the literatures, available to us.It was observed here that the LLT has produced better result with better acceptation by patients in the management of 'sinusitis' A LLT-treatment virtually produced no side-effect/complication as compared with the invasive methods.In this study. 25 cases, treated by LLT, out of 200 cases, treated by conventional methods, yielded good result in 22 & satisfactory result in 3 cases.
- Published
- 2002
- Full Text
- View/download PDF
38. Neuropharmacological profile of Barleria lupulina Lindl. Extract in animal models.
- Author
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Suba V, Murugesan T, Rao RB, Pal M, Mandal SC, and Saha BP
- Subjects
- Animals, Avoidance Learning drug effects, Avoidance Learning physiology, Behavior, Animal physiology, Central Nervous System drug effects, Central Nervous System physiology, Dose-Response Relationship, Drug, Female, Male, Mice, Muscle Relaxation drug effects, Muscle Relaxation physiology, Plant Components, Aerial, Plant Extracts isolation & purification, Plant Extracts pharmacology, Rats, Rats, Wistar, Acanthaceae, Behavior, Animal drug effects, Models, Animal
- Abstract
Barleria lupulina Lindl. is a popular medicinal plant distributed in mountains of southern and western India. In the present work, the effect of methanolic extract of aerial parts of B. lupulina on CNS activity has been evaluated. The CNS activity was tested in several experimental models, in mice and rats: general behavior, exploratory behavior, muscle relaxant activity, conditioned avoidance response and phenobarbitone sodium-induced sleeping time tests. The aerial parts of the plant B. lupulina was extracted with methanol and the solvent was removed by vacuum distillation. The methanol extract (100, 200 and 300 mg/kg) showed reduction in general behavioral pattern (spontaneous activity, alertness, awareness, pain response and touch response) in a dose dependent manner. The extract was found to produce a significant reduction of the exploratory behavioral profile (Y-maze test, head dip test) and conditioned avoidance response with all the tested doses. The methanolic extract showed significant motor incoordination and muscle relaxant activity. The extract also potentiated phenobarbitone sodium induced sleeping time. Preliminary investigation showed that the methanol extract of B. lupulina has significant psychopharmacological activity.
- Published
- 2002
- Full Text
- View/download PDF
39. Studies on the psychopharmacological activities of Rumex nepalensis Spreng. root extract in rats and mice.
- Author
-
Ghosh L, Arunachalam G, Murugesan T, Pal M, and Saha BP
- Subjects
- Animals, Female, Male, Maze Learning drug effects, Mice, Muscle Relaxants, Central administration & dosage, Muscle, Skeletal drug effects, Muscle, Skeletal physiology, Phenobarbital, Plant Extracts administration & dosage, Plant Roots, Psychotropic Drugs administration & dosage, Rats, Rats, Wistar, Sleep drug effects, Traction, Behavior, Animal drug effects, Muscle Relaxants, Central pharmacology, Phytotherapy, Plant Extracts pharmacology, Psychotropic Drugs pharmacology, Rumex
- Abstract
The methanol extract of Rumex nepalensis Spreng. (Family - Polygonaceae) was assessed for different psychopharmacological activities in rats and mice by evaluation of general behaviour, exploratory behaviour, muscle relaxant activity and phenobarbitone sodium-induced sleeping time. The extract at doses 200 mg/kg and above exhibited a reduction in spontaneous activity (general behavioural profile), a decrease in exploratory behavioural pattern in the Y-maze and Head-dip tests, a reduction in muscle relaxant activity in rotarod, 30 degrees inclined screen and traction tests, and also potentiated phenobarbitone sodium-induced sleeping time.
- Published
- 2002
- Full Text
- View/download PDF
40. Antidiarrhoeal activity of Strychnos potatorum seed extract in rats.
- Author
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Biswas S, Murugesan T, Sinha S, Maiti K, Gayen JR, Pal M, and Saha BP
- Subjects
- Animals, Antidiarrheals administration & dosage, Antidiarrheals therapeutic use, Dinoprostone pharmacology, Dose-Response Relationship, Drug, Female, Humans, Infant, Infant, Newborn, Male, Plant Extracts administration & dosage, Plant Extracts therapeutic use, Random Allocation, Rats, Rats, Wistar, Seeds, Antidiarrheals pharmacology, Diarrhea, Infantile drug therapy, Gastrointestinal Motility drug effects, Loganiaceae, Phytotherapy, Plant Extracts pharmacology
- Abstract
The antidiarrhoeal activity of the methanol extract of the dried seeds of Strychnos potatorum (MESP) has been evaluated out in rats using different models (castor oil-induced diarrhoea, effects on gastrointestinal motility and on PGE(2)-induced gastric enteropooling. MESP (100, 200 and 400 mg/kg, p.o.) significantly (P<0.001) inhibited the frequency of defaecation and reduced the wetness of faecal droppings in castor oil-induced diarrhoea, decreased the propulsion of charcoal meal through the gastrointestinal tract, and also reduced the PGE(2)-induced enteropooling.
- Published
- 2002
- Full Text
- View/download PDF
41. Review on phytochemical and medicinal aspects of jussiaea suferuticosa linn.
- Author
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Murugesan T, Sinha S, Pal M, and Saha BP
- Abstract
The whole plant of Jussiaea suffruticosa Linn. Has been widely used in traditional medicine of India. Important bioactive molecules of the plant extract have been explored with modern phytochemical approaches and reported to consist of betulinic acid, quercetin and β- sitosterol. Furthermore, the experimental data obtained from rational clinical studies indicated that the methanol extract of the whole plant has found to possess potent anti-HIV, anti-diabetic, anti-diarrheal, anti- inflammatory, anti-pyretic, diuretic and psychopharmacological activities in several animal models. This paper reveales the phytochemical and clinical importance of the plant extract.
- Published
- 2002
42. ANTIMICROBIAL ACTION OF THE LEAF EXTRACT OF Lagerstroemia parviflora Roxb.
- Author
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Mazumder A, Singn SK, Mazumder R, Basu SP, and Saha BP
- Abstract
The benzene extract of the leaves of Lagerstroemia paviflora Roxb was tested for its Minimum Inhibitory Concentration (MIC) against Gram Positive Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Klebsiella pneumoniae, Streptococcus pneumoniae, Lactobacillus arabinosus and gram negative strains E.Coli, Shigella dysenteriae, shigella sonnei, shigella boydii, Salmonella typhimurium, Proteus mirabilis and Vibrio cholerae. Further the zones of inhibition Produced by the crude extract against four selected bacterial strains were measured and compared with those produced by the standard antibiotic Ciprofloxacin against the same bacterial strains.
- Published
- 2002
43. Study on the diuretic activity of Strychnos potatorum Linn. seed extract in albino rats.
- Author
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Biswas S, Murugesan T, Maiti K, Ghosh L, Pal M, and Saha BP
- Subjects
- Animals, Chlorides urine, Female, Furosemide pharmacology, Male, Potassium urine, Rats, Rats, Wistar, Seeds chemistry, Sodium urine, Diuretics pharmacology, Loganiaceae, Plant Extracts pharmacology
- Abstract
Methanol extract of Strychnos potatorum Linn. seeds (SPSE) was evaluated for its diuretic activity in Wistar albino rats. The SPSE was administered at the graded doses of 200, 400, and 600 mg/kg body weight. The parameters which were taken into account during the experimental on each rat were: total urine volume (corrected for water intake during the test period), body weight before and after the experiment, and the concentration of sodium, potassium, and chloride ions in urine. The total urine volumes of the SPSE (600 mg/kg)-treated rats were evaluated nearly two and half fold then compared with the control (saline treated) group. Excretion of cations (sodium and potassium ions) and anions (chloride ions) also increased significantly with respect to the control group. The diuretic effect was comparable with that of the standard drug Furosemide. The increase of cations in the urine on treatment with Strychnospotatorum seed extract (SPSE) was dose-dependent. This effect supports the use of the Strychnos potatorum seeds as a diuretic in folk remedies.
- Published
- 2001
- Full Text
- View/download PDF
44. Studies on the hypoglycaemic activity of Punica granatum seed in streptozotocin induced diabetic rats.
- Author
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Das AK, Mandal SC, Banerjee SK, Sinha S, Saha BP, and Pal M
- Subjects
- Administration, Oral, Animals, Blood Glucose metabolism, Diabetes Mellitus chemically induced, Disease Models, Animal, Dose-Response Relationship, Drug, Female, Hypoglycemic Agents administration & dosage, Male, Plant Extracts administration & dosage, Rats, Rats, Wistar, Seeds, Streptozocin, Diabetes Mellitus drug therapy, Hypoglycemic Agents therapeutic use, Magnoliopsida, Phytotherapy, Plant Extracts therapeutic use
- Abstract
The hypoglycaemic activity of Punica granatum Linn. (Family Punicaceae) seed extract on rats made diabetic by streptozotocin (STZ) was investigated. The methanol extract of the seed at doses of 300 and 600 mg/kg, and chlorpropamide 200 mg/kg was administered to STZ diabetic rats. The seed extract (150, 300 and 600 mg/kg, orally) caused a significant reduction of blood glucose levels in STZ induced diabetic rats by 47% and 52%, respectively, at the end of 12 h., (Copyright 2001 John Wiley & Sons, Ltd.)
- Published
- 2001
- Full Text
- View/download PDF
45. Metabolism of 1,8-dihydroxy 3-hydroxy methyl anthraquinone (aloe-emodin) isolated from the leaves of Cassia tora in albino rats.
- Author
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Maity TK, Mandal SC, Bhakta T, Pal M, and Saha BP
- Subjects
- Animals, Anthraquinones, Emodin analogs & derivatives, Emodin blood, Emodin urine, Feces chemistry, Female, Male, Plant Extracts pharmacokinetics, Plant Leaves, Rats, Cathartics pharmacokinetics, Emodin pharmacokinetics, Fabaceae, Plants, Medicinal
- Abstract
Cassia tora is a well known plant of India. Aloe-emodin was isolated from the leaves of this plant and its metabolism pattern was studied. The results showed that about 15.4% of the administered aloe-emodin was excreted and the rest was probably bound or metabolized in the system., (Copyright 2001 John Wiley & Sons, Ltd.)
- Published
- 2001
- Full Text
- View/download PDF
46. Evaluation of anti-tussive activity of Bergenia ciliata Sternb. rhizome extract in mice.
- Author
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Sinha S, Murugesan T, Pal M, and Saha BP
- Subjects
- Administration, Oral, Animals, Antitussive Agents administration & dosage, Cough chemically induced, Dose-Response Relationship, Drug, Female, Male, Mice, Plant Extracts administration & dosage, Plant Roots, Sulfur Dioxide, Antitussive Agents therapeutic use, Cough drug therapy, Magnoliopsida, Phytotherapy, Plant Extracts therapeutic use
- Abstract
The methanol extract of the rhizome of Bergenia ciliata Sternb. (Saxifragaceae) has been evaluated for its potential in a cough model induced by sulphur dioxide gas in mice. The extract exhibited significant anti-tussive activity in a dose-dependent manner, as compared with control. The antitussive activity of the extract was comparable to that of codeine phosphate (10 mg/kg body wt.), a standard anti-tussive agent. The extract at doses of 100, 200 and 300 mg/kg body wt. (p.o.) showed significant inhibition of cough reflex by 28.7, 33.9 and 44.2%, respectively, within 90 min of the experiment.
- Published
- 2001
- Full Text
- View/download PDF
47. Antibacterial activity of Bergenia ciliata rhizome.
- Author
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Sinha S, Murugesan T, Maiti K, Gayen JR, Pal B, Pal M, and Saha BP
- Subjects
- Humans, Microbial Sensitivity Tests, Plant Extracts pharmacology, Plant Roots, Anti-Bacterial Agents pharmacology, Gram-Negative Bacteria drug effects, Gram-Positive Bacteria drug effects, Magnoliopsida, Plants, Medicinal
- Abstract
The methanol extract of Bergenia ciliata (tested at 200--1000 microg/disc) showed a wide spectrum of concentration-dependent antibacterial activity.
- Published
- 2001
- Full Text
- View/download PDF
48. Hepatoprotective activity of Cassia fistula leaf extract.
- Author
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Bhakta T, Banerjee S, Mandal SC, Maity TK, Saha BP, and Pal M
- Subjects
- Acetaminophen, Alanine Transaminase blood, Alkaline Phosphatase blood, Animals, Aspartate Aminotransferases blood, Bilirubin blood, Chemical and Drug Induced Liver Injury, Female, Hepatocytes enzymology, Hepatocytes pathology, India, Liver enzymology, Liver pathology, Male, Medicine, East Asian Traditional, Plant Extracts therapeutic use, Plant Leaves, Plants, Medicinal, Rats, Rats, Wistar, Fabaceae chemistry, Hepatocytes drug effects, Liver drug effects, Liver Diseases prevention & control, Plant Extracts pharmacology, Protective Agents pharmacology
- Abstract
Hepatoprotective activity of the n-heptane extract of Cassia fistula leaves was investigated by inducing hepatotoxicity with paracetamol in rats. The extract at a dose of 400 mg/kg body wt. exhibited orally, significant protective effect by lowering the serum levels of transaminases (SGOT and SGPT), bilirubin and alkaline phosphatase (ALP). The effects produced were comparable to that of a standard hepatoprotective agent.
- Published
- 2001
- Full Text
- View/download PDF
49. Pharmacognostical Profile of Roots of Rumex nepalensis spreng.
- Author
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Ghosh L, Gayen JR, Sinha S, Saha BP, and Pal M
- Abstract
The roots of Rumex nepalensis spreng (Family-Polygonaceae) were studied to fix the parameters for Pharmcognostical standards. The present investigation deals with the macrocopical characters of root as well as studies on some physical constants, behavior of powdered root on treatment with different chemical reagents and fluorescence characteristics on exposure to ultra -violet light. These studies would be of great help for proper identification of the root of the plant.
- Published
- 2001
50. Evaluation of anti-inflammatory potential of Bergenia ciliata Sternb. rhizome extract in rats.
- Author
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Sinha S, Murugesan T, Maiti K, Gayen JR, Pal M, and Saha BP
- Subjects
- Animals, Anti-Inflammatory Agents, Non-Steroidal isolation & purification, Carrageenan, Edema chemically induced, Edema drug therapy, Edema pathology, Female, Gossypium, Granuloma drug therapy, Granuloma pathology, Granuloma prevention & control, Male, Plant Extracts chemistry, Plant Extracts pharmacology, Plant Roots chemistry, Rats, Rats, Wistar, Serotonin, Anti-Inflammatory Agents, Non-Steroidal pharmacology, Plants, Medicinal chemistry
- Abstract
The methanol extract of the rhizome of Bergenia ciliata Sternb. (Saxifragaceae) has been evaluated for anti-inflammatory potential using two acute rat models (carrageenan- and serotonin (5-HT)-induced rat paw oedema) and a chronic rat model (cotton pouch-induced granuloma). Phenylbutazone (100 mg kg(-1)), a non-steroidal anti-inflammatory agent, was used as a standard. The methanol extract (100, 200 or 300 mg kg(-1)) exhibited significant (P < 0.05) anti-inflammatory activity in all the animal models. At 300 mg kg(-1) the methanol extract exhibited maximum inhibition of 32.4+/-2.89% in carrageenan-induced rat paw oedema while the standard showed an inhibition of 44.1+/-2.7% after 3 h of drug treatment. In the serotonin-induced rat paw oedema model, 300 mg kg(-1) methanol extract suppressed oedema by 45.33+/-2.09%, whereas the standard produced an inhibition of 53.5+/-4.3%. In the cotton pouch granuloma model the methanol extract inhibited significantly (P < 0.001) the granuloma weight in a dose-dependent manner. In this model, 300 mg kg(-1) extract produced a maximum inhibition of 31.4+/-1.09% in granuloma weight compared with 41.1+/-1.32% reduction in granuloma weight for the standard. The methanol extract of B. ciliata exhibited significant anti-inflammatory potential at the dose levels examined.
- Published
- 2001
- Full Text
- View/download PDF
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