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7. Development and validation of a UPLC-MS method for determination of atazanavir sulfate by the 'analytical quality by design' approach

Author

Saha Chandni, Gupta N. Vishal, and Chandan R. S.

Subjects
atazanavir sulfate, uplc-ms, “analytical quality by design”, Pharmaceutical industry, HD9665-9675
Abstract

A UPLC-MS method for the estimation of atazanavir sulfate was developed using the “analytical quality by design” approach. The critical chromatographic quality attributes identified were retention time, theoretical plates and peak tailing. The critical method parameters established were percent of organic modifier, flow rate and injection volume. Optimization performed using Box-Behnken Design (BBD) established 10 % organic modifier, 0.4 mL min−1 flow rate and 6-µL injection volume as the optimum method conditions. Atazanavir sulfate eluted at 5.19 min without any interference. Method validation followed international guidelines. The method has proven linearity in the range of 10–90 µg mL−1. Recovery was between 100.2–101.0 % and precision within the accepted limits (RSD 0.2–0.7 %). LOD and LOQ were 2.68 and 8.14 µg mL−1, resp. Stress testing stability studies showed atazanavir sulfate to degrade under acidic and basic conditions. The suggested technique is simple, rapid and sustainable. It is, therefore, suggested for routine analysis of atazanavir sulfate.

Published
2020
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8. Healthcare providers as patients: COVID-19 experience

Author

Al Mutair, Abbas, primary, Woodman, Alexander, additional, Al Hassawi, Amal I., additional, Ambani, Zainab, additional, Al Bazroun, Mohammed I., additional, Alahmed, Fatimah S., additional, Defensor, Mary A., additional, Saha, Chandni, additional, and Aljarameez, Faiza, additional

Published
2023
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11. Acceptance, Advocacy, and Perception of Health Care Providers on COVID-19 Vaccine: Comparing Early Stage of COVID-19 Vaccination with Latter Stage in the Eastern Region of Saudi Arabia

Author

Almusalami, Eman M., primary, Al-Bazroun, Mohammed I., additional, Alhasawi, Amal I., additional, Alahmed, Fatimah S., additional, Al-Muslim, Zahra M., additional, Al-Bazroun, Lubana I., additional, Muslim, Maryam, additional, Saha, Chandni, additional, Kay, Elbert, additional, Alzahrani, Zeyad A., additional, Ahmed, Gasmelseed Y., additional, and Al Mutair, Abbas, additional

Published
2023
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14. Clinical Outcomes and Severity of Acute Respiratory Distress Syndrome in 1154 COVID-19 Patients: An Experience Multicenter Retrospective Cohort Study

Author

Al Mutair, Abbas, primary, Alhumaid, Saad, additional, Layqah, Laila, additional, Shamou, Jinan, additional, Ahmed, Gasmelseed Y., additional, Chagla, Hiba, additional, Alsalman, Khulud, additional, Alnasser, Fadhah Mohammed, additional, Thoyaja, Koritala, additional, Alhuqbani, Waad N., additional, Alghadeer, Mohammed, additional, Al Mohaini, Mohammed, additional, Almahmoud, Sana, additional, Al-Tawfiq, Jaffar A., additional, Muhammad, Javed, additional, Al-Jamea, Lamiaa H., additional, Woodman, Alexander, additional, Alsaleh, Ahmed, additional, Alsedrah, Abdulaziz M., additional, Alharbi, Hanan F., additional, Saha, Chandni, additional, and Rabaan, Ali A., additional

Published
2022
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15. Assessment of nurses' knowledge, attitude and implementation of skin‐to‐skin care within the perinatal setting in Saudi Arabia: Survey study.

Author

Al Mutair, Abbas, Almutairi, Wedad, Aljarameez, Faiza, Kay, Elbert, Rabanal, Rhea, Abellar, Katrina, Napaod, Aireen, Hawsawi, Abeer, Saha, Chandni, and Ahmed, Gasmelseed Y.

Subjects
NURSING audit, MATERNAL health services, STATISTICS, RESEARCH, NURSES' attitudes, ATTITUDES of medical personnel, CROSS-sectional method, MULTIVARIATE analysis, NEONATAL nursing, PSYCHOLOGY of nurses, COMPARATIVE studies, T-test (Statistics), CRONBACH'S alpha, PEDIATRIC nurses, QUESTIONNAIRES, HEALTH attitudes, CRITICAL care medicine, DESCRIPTIVE statistics, SCALE analysis (Psychology), POSTNATAL care, SOCIODEMOGRAPHIC factors, DATA analysis software, STATISTICAL correlation, PATIENT positioning, EVIDENCE-based nursing
Abstract

Aim: To evaluate knowledge, attitude and implementation of Skin‐to‐Skin Care (SSC) among nurses and to assess the implementation of SSC in the perinatal setting. Design: A cross‐sectional study design was implemented utilizing survey distributed among critical care paediatric and neonatal nurses. Methods: The data collection form was pre‐structured validated tool consisted of two main sections; socio‐demographic characteristics and Likert scale of 20 items covering four main domains in; knowledge, attitude, education and implementation of SSC. Results: The vast majority of the sample 91 (98%) were females with mean age and mean years of experience 33.5 ± 6.5 and 9.7 ± 6.5 years respectively. Almost half of them 45 (48.4%) work in obstetric and labor and delivery units. Correlation coefficient revealed a significant association between the total educational years of nursing degree and SSC. The results showed nurses with bachelor or master significantly more knowledgeable and skilled in implementing SSC compared to others. [ABSTRACT FROM AUTHOR]

Published
2023
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