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In this paper, we advance the question: What are the semiotic (meaning-making) and learning opportunities offered by student-produced films? Our point of departure is a pedagogical project in which we invited management students to produce films on scalar issues related to global environmental change. Our analytical lens and our empirical findings (examined through a template analysis and a multimodal video transcription) help us make three contributions. First, we synthesize a new ontological and epistemological foundational "positioning" that portrays the nature of learning processes as revolving on meaning-making activities (a positioning that we have termed a performative, design-oriented multimodality). Second, we provide a framework that advances understanding of the "inner workings" of student film-making as resting on a triple engagement (cognitive-affective, behavioral, and relational), anchored on underpinning multimodal composing activities. These conceptual moves and empirical work allow us to distill several implications, or "provocations": film-making fosters radical openness in management learning curricula, gives new momentum to the instructor's role change ("from sage on the stage to guide on the side") and expands existing cultures of recognition around what gets valued and can be assessed as (signs of) learning. Third, we offer a pedagogical prototype containing practical teaching guidelines. [ABSTRACT FROM AUTHOR]

The decline in abundance and species richness of insects, including butterflies, have been linked to factors such as habitat loss and climate change. While some butterfly species are increasing, an overall decrease has been frequently observed in both Europe and North America. The objective of this study was to assess trends of butterfly abundances in Mediterranean shrublands of conserved lands in San Diego County, CA, USA. Funding and surveys were focused on the threatened Hermes copper (Lycaena hermes), but the abundance of all butterfly species was recorded. Analyses utilized the annual maximum count (Max Count) for each species at each transect during 2010–2022. The 10 most commonly observed species experienced, on average, a 1.4% annual decline in abundance, and 20 less commonly observed species were, on average, found at 5.9% fewer transects each year. The only exceptions to these declines are species (cabbage white [Pieris rapae], checkered white [Pontia protodice], and white checkered-skipper [Burnsius albezens]) that feed on non-native mustards or are more common in disturbed habitats. The Max Count provided an efficient, robust, and stable population index, that can be utilized to leverage funding for focal species to assess the broader community. [ABSTRACT FROM AUTHOR]

The study examined the protective effects of an aqueous extract of Salvia officinalis (Sage) in mice exposed to copper sulfate (CuSO4). Ten days of daily administration of 6 mg/kg CuSO4 were found to induce oxidative stress and neurotoxicity in the liver, brain, and kidney of mice. In fact, the activities of acetylcholinesterase (AChE), hydrogen peroxide (H2O2), and superoxide dismutase (SOD) decreased in the organs of mice. Furthermore, it was shown that CuSO4 caused a drop in cholesterol and triglyceride levels. Additionally, there was a general decrease in organ growth and body weight. Improvements in the antioxidant defense of the kidney and brain were observed to be promoted by the first concentration of 0.425 g/L (E1) of S. officinalis. In contrast, the second concentration of 1.275 g/L (E2) showed a greater improvement in SOD and AChE activities in liver. Sage extract (0.425 g/L) was able to raise the levels of AST, ALAT, and LDH in plasma. Additionally, it was noted that this concentration (E1) was beneficial for the growth of the three investigated organs as well as for preventing weight loss. [ABSTRACT FROM AUTHOR]

Expanding on work by Conway, Orson, and Powell, we study the isotopy classes rel. boundary of nonorientable, compact, locally flatly embedded surfaces in D 4 with knot group Z 2. In particular we show that if two such surfaces have the same normal Euler number, the same fixed knot boundary K such that | det ⁡ (K) | = 1 , and the same nonorientable genus 4 or 5, then they are ambiently isotopic rel. boundary. This implies that closed, nonorientable, locally flatly embedded surfaces in the 4-sphere with knot group Z 2 of nonorientable genus 4 and 5 are topologically unknotted. The proof relies on calculations, implemented in Sage, which imply that an obstruction to modified surgery is elementary. Furthermore we show that this method fails for nonorientable genus 6 and 7. [ABSTRACT FROM AUTHOR]

Better disease detection and classification for tomato leaves at early sage provide finest productivity results. It is a natural occurrence for tomato plants to get sick, and if the appropriate attention and corrective action are not given in a timely manner. Moreover, it negatively impacts the productivity, quality, and quantity of the corresponding product. It is crucial to check the health and look for disease in tomato crop leaves since, if handled, these issues can affect the plant, leading to significant losses at marketing fields. Thus this article proposed an advanced Self-developed Convolution Neural Network (CNN) with fewer layers than the other standard model and its accuracy was tested by varying different parameters. Consequently, results shows the highest accuracy obtained at the learning rate of 0.0003, 20 epochs, and on using the three different optimizer. All the experiments are performed on 4 classes such as yellow curl diseases, early blight, bacterial spot and healthy leaf of the plant village public dataset the highest accuracy of 99.67% achieved on 12 layers CNN model. [ABSTRACT FROM AUTHOR]

This research was carried out to determine the effects of different harvest time (HT) and salicylic acid application (SAA) on agronomic and quality characteristics of sage. The experiment was carried out in a randomized complete block design in split plots with HTs (pre-flowering and 50% flowering periods) in the main plots and SAAs in the subplots (Control, 1, 2, and 4 mM). Plant height, fresh herb yield, dry herb yield, fresh leaf yield, dry leaf yield, leaf ratio, essential oil (EO) content, EO yield, and EO composition were examined. In total or mean results, the HT was statistically significant on plant height, fresh and dry herb yield, leaf ratio in both years. The SAA was statistically significant on fresh and dry herb yield and leaf ratio only in the second year. Among the 24 components identified, alpha thujone was the main component. This component was followed by camphor, 13-epimanool, veridiflorol, 1.8-cineol. According to the principal component analysis (PCA), in 2021, 1 mM SAAs applied at the 50% flowering period in the first cutting and at the pre-flowering period in the second cutting showed different properties regarding EO composition. In 2022, 4 mM SAAs applied at the 50% flowering period in the first and second cuttings showed different properties regarding EO composition. The study found that delaying the HT led to significant increases in plant height, fresh and dry herb yields. Additionally, the SAA increased the herb yield and decreased the leaf ratio, particularly in the second year. [ABSTRACT FROM AUTHOR]

Background: The use of complementary and alternative medicine (CAM) has increased substantially around the world; various global studies have documented the use of CAM by surgical patients in the preoperative period, with rates of herbal medications and other nonherbal treatments ranging from 12 to 69%. This study aimed to identify the prevalence of CAM and its patterns of use among surgical patients. Methods: From December 2020 to April 2021, a descriptive cross-sectional study was undertaken in Palestine. A face‒to-face interview questionnaire was used. The participants were asked questions regarding sociodemographics, clinical information, CAM usage, reasons for its use, and opinions on its efficacy. Results: Among the 300 surgical patients who were interviewed in this study, 252 (84.0%) reported that they had ever used CAM, while a total of 122 (48.4%) had used CAM before their surgery, 81.7% of the respondents used herbal methods, and 64.7% of them used nonherbal methods. CAM use among our study population was not linked to specific demographics or other characteristics, except health insurance (p = 0.004). The most commonly used herbs among surgical patients are Salvia officinalis L. (Sage) (84.1%) and Pimpinella anisum L. (Anise) (75.5%). The highest percentage was found among patients with university qualifications (73.2%), who used more than three methods (p < 0.001). In addition, 59.4% of the females used more than three therapies, whereas 45.9% of the males did (p = 0.032). Conclusions: The herbal methods used are common among surgical patients in Palestine and are consistent with the enormous increase in the use of CAM. Therefore, awareness should be raised among the public regarding the risks and benefits of CAM use. [ABSTRACT FROM AUTHOR]

The article in the journal "Fabula" is an obituary for Leander Petzoldt, a prominent figure in narrative research blending literary studies and folklore. Petzoldt's extensive work focused on the interplay between literature and folk narratives, particularly in the context of Germanic storytelling traditions. His research contributions spanned various aspects of narrative analysis, including the study of myths, legends, and folk tales, making him a respected scholar in the field of European folklore studies. [Extracted from the article]

Mediterranean‐climate shrublands are key biodiversity hotspots and carbon storage pools, but are increasingly threatened by climate change, non‐native species, and altered fire regimes. Fires are important to historic shrubland disturbance cycles but can also promote non‐native plants, which may limit post‐fire native shrub recovery. Increasing drought with climate change could also reduce post‐fire shrub regeneration. I developed a stochastic, individual‐based demographic model (IBM) for the native shrub Artemisia californica, parameterized from an experimental removal of non‐native annuals after a 2013 fire in southern California. The IBM simulated A. californica recovery for 7 years after fire under different rainfall conditions (drought or pre‐drought) and non‐native removal strategies (from no years to all 7 years). Drought lowered A. californica canopy volume 7 years after fire by 90% or more. Rainfall in the second year after fire, when most A. californica germination occurred, had particularly strong effects on final canopy cover. Non‐native removal in all 7 years increased canopy volume by three times under drought conditions and 3.5 times under pre‐drought conditions. Targeting non‐native removal in the first 2 years proved nearly as effective, achieving from 88% (drought) to 95% (pre‐drought) the benefits of removal in all 7 years. In sum, low rainfall may be the most important limitation on post‐fire shrub recovery, but removal of non‐natives in years of pulsed shrub recruitment can be an effective restoration strategy even under drought conditions. More generally, this study illustrates how demographic models can help optimize the targeting of scarce management and restoration resources. [ABSTRACT FROM AUTHOR]

The cultivation of Salvia lavandulifolia, Spanish sage, makes an important contribution to the economy of many rural areas in Southeastern Spain. This aromatic plant species is characterized by high intraspecific variability, which makes the selection process for the establishment of homogeneous crops difficult. Additionally, imminent climate change threatens to reduce its production, especially when cultivated in drylands. Therefore, to guarantee the continued production of this type of sage, it is essential to study its agronomic behavior and production quality. For this, clones from four ecotypes were cultivated for three years, assessing changes in their biomass production, essential oil yield and quality, and phenolic fraction, as well as the corresponding antioxidant activity. The results suggest that essential oil yield is genetically predetermined, greater biomass not being associated with higher quantities of essential oil. Weather conditions affected both essential oil and phenolic fraction secondary metabolism. Under very harsh conditions, Spanish sage produces higher concentrations of camphor and 1,-8-cineole along with luteolin-7-O-glucoside, and lithospermic, rosmarinic, and salvianolic A acids in its phenolic fraction. The synthesis of these components helps the species to withstand the hot and dry conditions typical of southeast Spain. [ABSTRACT FROM AUTHOR]

Drought is one of the most important abiotic factors limiting plant productivity. Although the aromatic plants of the Lamiaceae family often grow in arid regions, drought tolerance varies greatly among the different species of this family. The effect of induced drought stress can be reduced by the application of selenium. The current study aims to compare the growth and biochemical responses of three species of the Lamiaceae family (hyssop, salvia, and oregano) to drought stress and the possibility of reducing the effect of stress in these plants by foliar treatment with selenium. Drought stress reduced the fresh and dry biomass of hyssop (by 35% and 15%), salvia (by 45% and 41%), and oregano (by 51% and 32%). Se treatment did not affect the growth of plants under drought stress, but it improved relative water content in hyssop and salvia under moderate drought conditions. A reduction in the content of chlorophyll a and chlorophyll b (in hyssop and salvia). In addition, an increase in the content of hydrogen peroxide (in oregano and salvia), malondialdehyde, and proline in plants cultivated under drought conditions was observed. Se treatment led to reduced levels of hydrogen peroxide and malondialdehyde, along with an increase in chlorophyll a content (in hyssop and oregano) and proline content. The response of the antioxidant system depended on the plant species. Hyssop exhibited a significant increase in glutathione peroxidase, superoxide dismutase, and peroxidase activities. Oregano showed enhanced catalase activity. Salvia experienced a sharp increase in ascorbic acid content. Se treatment stimulated the accumulation of phenolic compounds and increased glutathione peroxidase activity in all studied species. [ABSTRACT FROM AUTHOR]

A combination of network pharmacology, molecular docking and ADME/drug-likeness predictions was employed to explore the potential of Salvia officinalis compounds to interact with key targets involved in the pathogenesis of T2DM. These were predicted using the SwissTargetPrediction, Similarity Ensemble Approach and BindingDB databases. Networks were constructed using the STRING online tool and Cytoscape (v.3.9.1) software. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analysis and molecular docking were performed using DAVID, SHINEGO 0.77 and MOE suite, respectively. ADME/drug-likeness parameters were computed using SwissADME and Molsoft L.L.C. The top-ranking targets were CTNNB1, JUN, ESR1, RELA, NR3C1, CREB1, PPARG, PTGS2, CYP3A4, MMP9, UGT2B7, CYP2C19, SLCO1B1, AR, CYP19A1, PARP1, CYP1A2, CYP1B1, HSD17B1, and GSK3B. Apigenin, caffeic acid, oleanolic acid, rosmarinic acid, hispidulin, and salvianolic acid B showed the highest degree of connections in the compound-target network. Gene enrichment analysis identified pathways involved in insulin resistance, adherens junctions, metabolic processes, IL-17, TNF-α, cAMP, relaxin, and AGE-RAGE in diabetic complications. Rosmarinic acid, caffeic acid, and salvianolic acid B showed the most promising interactions with PTGS2, DPP4, AMY1A, PTB1B, PPARG, GSK3B and RELA. Overall, this study enhances understanding of the antidiabetic activity of S. officinalis and provides further insights for future drug discovery purposes. [ABSTRACT FROM AUTHOR]

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of Spanish sage oil from the leaves of Salvia officinalis ssp. lavandulifolia (Vahl) Gams (Spanish sage oil) when used as a sensory additive in feed and in water for drinking for all animal species. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the additive under assessment is considered safe up to the maximum use level of 14 mg/kg complete feed for all animal species. The FEEDAP Panel considered that the use of Spanish sage oil in water for drinking is safe provided that the total daily intake of the additive does not exceed the daily amount that is considered safe when consumed via feed. The use of Spanish sage oil in animal feed under the proposed conditions of use is safe for the consumer and the environment. Regarding user safety, the essential oil under assessment should be considered as an irritant to skin and eyes and as a dermal and respiratory sensitiser. Since the oil of the leaves of S. officinalis ssp. lavandulifolia (Vahl) Gams is recognised to flavour food and its function in feed would be essentially the same as that in food, no further demonstration of efficacy was considered necessary. [ABSTRACT FROM AUTHOR]

Simple Summary: Drosophila suzukii (Diptera: Drosophilidae) is an invasive pest that lays eggs in ripe fruits. The short generation time, high fertility, and wide host range allows this species to cause important yield losses. Essential oils, extracted from a number of plant species, have been studied for their attractiveness to and deterrence of D. suzukii. In this study, we found clary sage oil exhibited dose-dependent repellency against both D. suzukii adults and larvae. Also, we identified that linalyl acetate and linalool were two major constituents of clary sage oil by GC-MS. Furthermore, we detected that both linalyl acetate and linalool repelled D. suzukii adults and larvae. In addition, we examined the electroantennography (EAG) responses of D. suzukii to clary sage oil, linalyl acetate, and linalool. These results suggest clary sage oil and its constituents linalyl acetate and linalool could be potential repellents for the management of D. suzukii. Drosophila suzukii (Diptera: Drosophilidae), spotted-wing drosophila, poses a significant threat to soft-skinned fruit crops in the Americas, Europe, Africa, and Oceania, as well as in Asia. The application of chemical insecticides is the primary control strategy for D. suzukii; however, resistance has developed with the indiscriminate use of chemical insecticides. Essential oils, considered potential alternatives to pesticidal strategies, exhibit potent toxic and sublethal behavioral effects against numerous pests, including D. suzukii. Clary sage oil repels a variety of agricultural and household pests; however, whether it has a repellent effect against D. suzukii remains unknown. Here, we found that clary sage oil exhibited dose-dependent repellency against D. suzukii adults in a T-maze assay, a two-choice assay and a two-choice attraction assay. Also, clary sage oil showed a significant repellent effect against D. suzukii larvae. Next, we explored the chemical constituents of clary sage oil by GC-MS and identified two major constituents, linalyl acetate (40.03%) and linalool (23.02%). Furthermore, the behavioral assays of linalyl acetate and linalool showed that both compounds conferred comparable repellency against D. suzukii adults and larvae. Finally, we found clary sage oil, linalyl acetate, and linalool elicited EAG responses in D. suzukii, especially clary sage oil, suggesting the repellency was mediated by the olfactory system. These findings indicate that D. suzukii shows olfactory-based behavioral avoidance of clary sage oil, linalyl acetate, and linalool. Clary sage oil and its major constituents may be possible alternatives in the management of D. suzukii. [ABSTRACT FROM AUTHOR]

Artificial light at night is increasingly recognized as a cause of habitat degradation, and as a conservation threat to wildlife species. We studied the behavioral response of Pacific kangaroo rats (Dipodomys agilis) to natural and artificial light in coastal sage scrub in southern California, United States. We used digital trail cameras to monitor visits to seed trays in shrub and open, intershrub microhabitats. Under natural-light conditions, kangaroo rats were more active on full- than new-moon nights, and concentrated foraging beneath shrubs. Surprisingly, kangaroo rats frequently interacted with other rodents in seed trays. Near to an artificial light source, kangaroo rats removed fewer seeds, had fewer foraging bouts, and spent less time in trays—especially in brightly lit open microhabitats—compared to distances farther from the light. Differences between the first and second nights of trials during both experiments suggested that kangaroo rats learned locations of resource-rich seed trays and modified their behavior accordingly. Our results are similar to recent studies showing that artificial light depresses foraging activity of rodents in the areas of highest illumination, and adds to evidence of the need to mitigate impacts of urban lighting in fragments of coastal sage scrub, especially along the urban–wildland interface. [ABSTRACT FROM AUTHOR]

Background: Diviner's sage (Salvia divinorum; Lamiaceae) is the source of the powerful hallucinogen salvinorin A (SalA). This neoclerodane diterpenoid is an agonist of the human Κ-opioid receptor with potential medical applications in the treatment of chronic pain, addiction, and post-traumatic stress disorder. Only two steps of the approximately twelve step biosynthetic sequence leading to SalA have been resolved to date. Results: To facilitate pathway elucidation in this ethnomedicinal plant species, here we report a chromosome level genome assembly. A high-quality genome sequence was assembled with an N50 value of 41.4 Mb and a BUSCO completeness score of 98.4%. The diploid (2n = 22) genome of ~ 541 Mb is comparable in size and ploidy to most other members of this genus. Two diterpene biosynthetic gene clusters were identified and are highly enriched in previously unidentified cytochrome P450s as well as crotonolide G synthase, which forms the dihydrofuran ring early in the SalA pathway. Coding sequences for other enzyme classes with likely involvement in downstream steps of the SalA pathway (BAHD acyl transferases, alcohol dehydrogenases, and O-methyl transferases) were scattered throughout the genome with no clear indication of clustering. Differential gene expression analysis suggests that most of these genes are not inducible by methyl jasmonate treatment. Conclusions: This genome sequence and associated gene annotation are among the highest resolution in Salvia, a genus well known for the medicinal properties of its members. Here we have identified the cohort of genes responsible for the remaining steps in the SalA pathway. This genome sequence and associated candidate genes will facilitate the elucidation of SalA biosynthesis and enable an exploration of its full clinical potential. [ABSTRACT FROM AUTHOR]

• Salvia multicaulis (wild) × Salvia officinalis (cultivated) produced F1 hybrid. • F1 hybrid has the highest content of rosmarinic acid and quercetin. • PAL and CYP expressions strongly correlate with rosmarinic acid content. • Cineole contents negatively correlate with the expression of the CS gene. • Salvia has antioxidant, anticancer, anti-inflammatory, antibacterial effects. The Salvia genus, particularly the wild genotypes, received significant attention due to its antibacterial, antioxidant, antiviral, and anticancer properties. Crossbreeding wild and cultivated plants can conserve the genetic resources of wild plants and develop new hybrids with high yields and high resistance to climatic changes. In this study, Salvia multicaulis (P1, wild) and Salvia officinalis (P2, cultivated) have been crossed to produce a novel hybrid (F1 hybrid) in the Saint Catherin protectorate - Egypt. In P1, P2, and F1 hybrid, heterosis, phytochemical composition, gene expression of PAL (Phenylalanine ammonia-lyase), RAS1 (Rosmarinic acid synthase I), CYP (Cytochrome P450), and CS (1,8-Cineole synthase), besides antioxidant, anticancer, anti-inflammatory, and antibacterial effects were assessed. The F1 hybrid recorded the highest means of plant height in the two seasons. Heterosis over the mid-parents was positively associated with various traits, including stem thickness, number of branches, fresh yield, and percentage of oil in both seasons. Total phenolic and flavonoid content were found in the following order: P2 > P1 > F1. DPPH antioxidant effect was found in the following order: P2 > F1 > P1. The F1 hybrid had the highest content of rosmarinic acid (12.379 mg/g), quercetin (0.595 mg/g), and naringenin (0.152 mg/g), followed by P1 and P2. The 1,8-cineol and α-pinene were the main compounds in the essential oils of P1, P2, and F1. The 1,8-cineol was found in the following order: P2 > F1 > P1, while α-pinene was found in the following order: P1 > F1 > P2. The RAS1 expression showed a weak correlation with the concentration of rosmarinic acid, while the expressions of PAL and CYP were strongly correlated with the concentrations of main phenolics, including rosmarinic acid, quercetin, and naringenin. The 1,8-cineol content was negatively correlated with the expression of the CS gene. The P2 extract and the F1 hybrid essential oil showed potent anticancer, anti-inflammatory, and antibacterial properties. Crossbreeding S. multicaulis (P1, wild) and S. officinalis (P2, cultivated) in this study developed a new hybrid (F1) with good growth characteristics, high essential oil yields, high levels of bioactives, and good bioactivity. This makes it suitable for large-scale production for use in food and pharmaceutical applications. [ABSTRACT FROM AUTHOR]

Establishing a relationship between the witch or witchdoctor and the sage is not immediately evident. To most modern men the witch is simply a wretched wight devoid of knowledge and insight of modern life, whereas the sage or the intellectual is the great thinker, a custodian of Western ideologies and theories who supposedly does not believe in witchcraft and even questions the existence of God. The word sage is derived from the Latin verb sapere whose other meaning is “to have a good taste”. On the contrary, when one goes back in space and time to the period referred to as “once upon a time” in fairy tales, one discovers that the witch and the sage were indivisible; in fact they were one and the same person. This is clearly evinced in the etymology of some words of Indo-European origin. In fact, the distinction between witch and sage is flimsy and transparent, sharing the same semantic area with the following words: wizard, druid, fili, wicca, sorcerer, juggler, powwow, lamia and lilith. To the modern mind it strains belief to hear that the span of the word witch has roots in the sacred domain, that of priesthood, shamanism and sainthood for example. The truth is that the sage and the witch emerged from a common past. For example, the early pre- Socratic philosophers were shamanic practitioners first and wise men second. The parting of the ways is not as clear-cut in Arabic cultures where the ulana are learned in sacred scriptures and at the same time, learned in science, thus harking back to the past when the duality between sacred and secular did not exist. The medieval alchemist, forerunner of today’s scientist, is another case in point; and of course it is well-known that in many old cultures the priest class or caste were not only repositories of religious authority and initiated into sacred mysteries, but living repositories of all forms of specialised knowledge, including the art of writing. Acknowledged philosophers and writers among which Virgil, Shakespeare, Hugo, Apollinaire also write about it.

Introduction: Liver diseases represent a significant global health challenge, with primary causes including excessive alcohol consumption, infections, chemotherapy, and autoimmune disorders. Medicinal plants, due to their natural bioactive compounds, hold promise for developing effective treatments and preventive measures against liver ailments. This study aimed to document the use of herbal remedies in northeastern Morocco for liver diseases and correlate these uses with scientific evidence through a bibliometric analysis. Methods: An ethnobotanical survey was conducted in remote communities of northeastern Morocco from October 2020 to January 2022. A total of 189 informants were interviewed using semi-structured questionnaires to gather information on local medicinal plants used for liver ailments. The data were analyzed using four ethnobotanical quantitative indices: use value (UV), familial use value (FUV), informant consensus factor (ICF), and fidelity level (FL). Additionally, a bibliometric analysis was performed to evaluate the scientific support for the ethnopharmacological uses documented. Results: The survey identified 45 plant species from 26 different families used in the treatment of liver diseases. The most frequently utilized species were Cuminum cyminum L. (UV = 0.1065), Allium sativum L. (UV = 0.1015), Salvia officinalis L. (UV = 0.0761), Asparagus officinalis L. (UV = 0.0558), and Ziziphus lotus (L.) Lam. (UV = 0.0457). The Apiaceae family showed the highest familial use value (FUV = 0.1066), followed by Alliaceae (FUV = 0.1015). Liver congestion had the highest informant consensus factor (ICF = 0.83), followed by hepatic colic (ICF = 0.80). Bibliometric analysis revealed that 61% of the plants identified had documented pharmacological effects related to liver health. Discussion: The study demonstrates that traditional knowledge in northeastern Morocco encompasses a rich diversity of medicinal plants used to treat liver diseases. The high ICF values indicate a strong consensus among informants on the efficacy of these remedies. The correlation between ethnopharmacological use and scientific validation for a significant portion of these plants suggests their potential as reliable therapeutic agents for liver conditions. However, further scientific investigations are necessary to confirm their efficacy and safety in clinical settings. This research contributes valuable information for future studies on the therapeutic potential of these plants. Conclusion: This ethnobotanical survey provides a comprehensive database of medicinal plants used in northeastern Morocco for liver diseases. The findings highlight the potential of these plants in developing novel treatments for hepatic conditions, although further research is essential to substantiate their therapeutic claims. [ABSTRACT FROM AUTHOR]

This research has investigated the effect of different concentrations of nanoparticles (zinc, iron, titanium) and smoke-water on some growth and physiological characteristics of the medicinal plant sage. A factorial experiment was conducted in the form of a randomised complete block design with three replications in the research greenhouse of the Campus of Agriculture and Natural Resources, Razi University, Iran, over two consecutive years, 2021 and 2022. The first factor included foliar spraying of nanoparticles of Fe, Zn, Ti with two levels: 0 and 50 mg l−1. The second factor was foliar spraying using smoke-water with two levels of 0.5 and 1 mg l−1. Treatments were applied in three stages after graft establishment. Results indicated that the highest stem dry weight (68.477 g) occurred with 0.5 mg l−1 smoke-water without nanoparticles. At 0 mg l−1 smoke-water, titanium (64.493 g) and iron (65.193 g) nanoparticles yielded the highest stem weights. Application of 0.5 mg l−1 of smoked water improved the dry weight of leaves and stems. Foliar spraying of 50 mg l−1 of zinc and titanium nanoparticles also increased growth characteristics and chlorophyll content of sage leaves. In both cuttings, different concentrations affected some growth characteristics and photosynthetic pigments of sage plants. [ABSTRACT FROM AUTHOR]

The purpose of the experiment was to determine the effect of the addition of dried ground whole leaves of the herb Salvia officinalis L in the feed for fattening rabbits, on the productive parameters, some blood indicators and carcass characteristics. The inclusion of ground dry leaves of the herb sage in feed for fattening rabbits has a positive effect on productive performance. In the experimental group were improved average daily gain by 29,2% (P<0,05), feed conversion ratio by 19% (P<0,05) for the entire experimental period and average daily feed consumption by 3,8% (P<0,05) during the first week of the experiment compared to the control.The tested additive did not reliably affect the parameters of the slaughter analysis, the chemical analysis of the meat and blood parameters. [ABSTRACT FROM AUTHOR]

Aims: Post‐fire vegetation recovery is often determined by the similarity of post‐burn with unburned sites because of a lack of in situ information on pre‐fire communities. The inclusion of pre‐fire data can help account for pre‐existing differences and explore recovery also in terms of return to pre‐fire conditions. We used long‐term monitoring data in coastal sage scrub and grasslands to: (a) examine vegetation cover recovery of different functional groups; and (b) determine whether vegetation composition in burned areas has recovered in 4 years after fire with burned to unburned and pre‐ to post‐fire comparisons. Location: Orange County, California, USA. Methods: We analyzed long‐term vegetation monitoring (2007–2021) data from 39 grassland and 58 coastal sage scrub transects in southern California, including observations before and after the 2017 Canyon 2 fire. Linear mixed‐effect models were used to determine whether forb, grass, and shrub covers differed between burned and unburned sites while considering the effects of year and repeated monitoring. We used canonical analysis of principal coordinates to analyze vegetation composition based on burn status and time of sampling. Results: Whereas vegetation cover in grassland recovered quickly, native vegetation cover in burned coastal sage scrub remained lowered 4 years after fire, though forb and non‐native grass cover were higher in some post‐fire years. Community composition in burned coastal sage scrub was still in recovery 4 years after fire when compared with unburned or pre‐fire composition. Although burned and unburned grassland differed after fire in dominant grass species, inclusion of pre‐fire data showed that this was a pre‐existing difference. Conclusions: Coastal sage scrub had not recovered pre‐fire vegetation cover and composition by 4 years after fire, whereas grassland cover rebounded quickly, albeit with shifts in composition over time; patterns that were detected only by having pre‐ and post‐fire data from long‐term monitoring efforts. [ABSTRACT FROM AUTHOR]

Background: Viruses significantly affect global health. The few available viricidal and antiviral therapies are expensive and often associated with unwanted side effects. Functional foods as plant extracts (nutraceuticals) are epigenetically active, and multi-target antiviral compounds that affect several steps of the viral life cycle and host proteins. MicroRNAs (miRNAs) post-transcriptionally regulate host and viral gene expression and are responsible for the fine-tuning of gene expression by controlling the expression of their target messenger RNAs (mRNA) in host cells and viruses. Several plant ingredients have been proven to be active against RNA virus infections. Obviously, miRNAs play a central role in the regulation of gene transcription in viral replication and host immune defense. A healthy diet and nutraceuticals can support the fine-tuning of miRNAs. Objectives: The aim of this study was to analyze the effects of epigenetic active phytoceuticals and/or functional foods on immune relevant miRNAs and mRNAs in a healthy human study population. Methods: In a 2-month human intervention study (n=80), a mix of plant extracts (sage extract, green tea extract, berberine, apple extract, onion extract, elderberry extract, and grape skin extract) with proven activity against RNA viruses were examined for the change of miRNA and mRNA patterns involved in virus response systems. Lifestyle and nutritional behavior, including self-reported stress levels, as well as infection frequency as potential cofounders have been raised. Results: The intervention with secondary plant extracts could modulate the expression of miRNAs involved in the virus defense (miR-30e, miR-877, miR-150) and the expression of NF-κB. The intervention also increased miRNA expression patterns associated with a positive lifestyle. The intervention adapted miRNAs that correlate with an increased risk of infection, smoking, stress, and processed meat products. Conclusion: Plant extracts can enhance the beneficial effects of a healthy nutrition and lifestyle by modulating miRNAs. MiRNAs are promising drug targets for maintaining immune homeostasis. [ABSTRACT FROM AUTHOR]

The extant texts of Enochic Judaism present accounts about the early history of humankind, which use the motifs familiar from the ancient Mesopotamian historiography. The different versions of the Jewish story of the Watchers originate as counter narratives about the antediluvian sages, or apkallus, which are known from the cuneiform literature. The myth of Adapa in Akkadian offers a version of the narrative in which the sage was promoted in heaven to a god-like status, which concept is corroborated with an entry in the cuneiform compendium Tintir and the Chronicle of the Esagila. This tradition also associated Adapa with the immortalized flood survivor. In the form of a counter narrative, the Enochic texts of Judaism attributed the heavenly assumption to Enoch and presented the Watchers as the demonic entities subordinated to him. The fall of the Watchers, the promotion of Enoch, and the primordial battle against evil forces are depicted in the Animal Apocalypse of 1 Enoch 85–90. A very similar scenario is found in the Chronicle of Esagila, which describes the early history of humankind from the Babylonian perspective. It can be demonstrated that the Animal Apocalypse uses this or a similar cuneiform historiographic source as the basis of its counter narrative. [ABSTRACT FROM AUTHOR]

The plant-mediated synthesis of therapeutic metal nanoparticles is an intensively exploited field in the last decade. In particular, Salvia officinalis, considered one of these plants, was used in this work to synthesize silver particles. Here, we have used harmless substances to obtain silver particles and common characterization methods for quickly estimating sizes and shapes. Thus, UV–Visible spectroscopy helped us online-monitor and optimize the synthesis of silver particles and estimate the size of metallic particles in the stock solutions. The resulting eco-friendly synthesized silver particles were then separated and re-dispersed in water, to be analyzed by laser light scattering, transmission electron microscopy (TEM), and scanning electron microscopy (SEM) to prove their nanometric size and shape polydispersity. Furthermore, the role of citric acid in stabilizing colloidal solutions of silver nanoparticles was studied. [ABSTRACT FROM AUTHOR]

Background: The long history of the Palestinian-Israeli conflict has resulted in a public and mental health crisis. Despite the significant mental health burdens facing the Palestinian population in the Occupied Palestinian Territories (OPT) and refugee camps, this issue remains comprehensively unexplored. Aims: This scoping review identified mental health interventions and assessed their effectiveness among populations in the OPT and Palestinian refugee camps, while addressing delivery challenges. Method: A scoping review was conducted using 14 databases to include relevant studies published through March 2024 using PRISMA guidelines for scoping reviews. Results: Analyzing 31 intervention studies meeting the inclusion/exclusion criteria, revealed varied outcomes in interventions targeting post-traumatic stress disorder, depression, and overall mental well-being. Conclusions: While some interventions showed potential, others had limited effectiveness, underscoring the complexity of mental health needs in conflict zones. Gender- and risk-specific effectiveness was observed, emphasizing the need for tailored approaches. Challenges like low attendance rates and systemic barriers hindered intervention success. The profound impact of war on children stresses the urgency for targeted interventions. Empowerment and community connectedness are vital for resilience, along with the importance of family and community involvement and research that balances the need for strong evaluation designs with the need for ongoing mental health services. Advocacy for systemic changes is crucial to implement the complex sustainable interventions necessary to assure mental health in any population. This review highlights the importance of holistic, contextually relevant approaches for mental health intervention in OPT and refugee camps, emphasizing rigorous evaluations and community-driven approaches. [ABSTRACT FROM AUTHOR]

Extracts of Salvia officinalis (S. officinalis) have been described to have many therapeutic properties. However, the effect of S. officinalis on copper sulfate toxicity has not been previously reported. The aim of this study was to investigate the toxicity of copper sulfate and the potential beneficial effects of S. officinalis aqueous infusion on proinflammatory response and antioxidant status. 56 male mice were used and equally divided into 6 groups: control group, copper sulfate treated group (40 mg/kg), S. officinalis aqueous infusion treated groups (200 mg/kg and 400 mg/kg) separately or in combination with copper. IL-6 (interleukine-6) and TNF-α (Tumor necrosis factor alpha) were assessed by Elisa. Catalase (CAT), superoxide dismutase (SOD) and acetylcholinesterase (AChE) activities, malondialdehyde (MDA) and oxygen peroxide levels were determined. Serum biochemical parameters were analyzed. Copper enhanced aspartate aminotransferase (AST), alanine aminotransferase (ALT) and Lactate dehydrogenase (LDH) (p < 0.05). Copper enhances significantly IL-6, TNF-α and MDA levels in liver and kidney and reduced CAT, SOD and AChE activities (p < 0.05). Aqueous infusion of S. officinalis at 400 mg/kg abolished copper-induced changes in AST and ALT activity. S. officinalis aqueous infusion at 200 mg/kg reversed copper-induced IL-6 in kidney and TNF-α in liver at both doses. S. officinalis aqueous infusion at 400 mg/kg restored SOD in kidney and CAT and AChE activities in both liver and kidney. S. officinalis aqueous infusion may be useful in partially ameliorating tissue disorders induced by copper exposure such as inflammatory response, oxidative stress imbalance and organ dysfunction through its phenolic compounds and higher antioxidant capacity. [ABSTRACT FROM AUTHOR]

Salvia officinalis L., commonly known as sage and belonging to the Lamiaceae family, is a medicinal herb indigenous to the Mediterranean region. It is celebrated for its diverse pharmacological properties and traditional uses in folk medicine, particularly in addressing hepatotoxicity. Cisplatin (Cis), a potent chemotherapeutic agent widely employed in cancer treatment, is recognized for its efficacy but often accompanied by adverse effects, including hepatotoxicity. The aim of this study was to assess whether an ethanolic S. officinalis extract (ESOE) could provide protection against Cis -induced hepatotoxicity in an experimental rat model. The ESOE was prepared using standard extraction techniques, and its chemical constituents were elucidated through UPLC-ESI-MS/MS analysis, revealing the presence of bioactive compounds such as alkaloids, phenolic compounds, and flavonoids, which are associated with various therapeutic effects, including hepatoprotection. Adult male albino rats were allocated into four groups: control, ESOE (250 mg/kg), Cis (7.5 mg/kg), and ESOE (250 mg/kg) + Cis (7.5 mg/kg). The treatment duration lasted 21 days, with Cis administration on the 22nd day. Twenty-four hours post-Cis administration, blood and liver samples were collected for analysis. Cis -induced hepatotoxicity was evidenced by alterations in hematological parameters, including erythrocyte, thrombocyte, leukocyte, and lymphocyte counts, alongside elevated serum levels of liver enzymes (ALT, LDH, AST, ALP, and GGT), indicative of liver damage. Furthermore, Cis exposure resulted in increased hepatic malondialdehyde (MDA) and Nitric oxide (NO) levels, oxidative stress markers, coupled with decreased levels of reduced glutathione (GSH), a non-enzymatic antioxidant, and histopathological changes in liver tissue, characterized by necrosis and inflammation. Additionally, Cis treatment led to elevated levels of 8-hydroxy-2′-deoxyguanosine (8-OH-dG), TNF-α, and IL-6, indicating oxidative stress and inflammation. Remarkably, pretreatment with ESOE ameliorated these Cis -induced hepatotoxic effects, as evidenced by improved hematological parameters, reduced liver enzyme activities, alleviated oxidative stress, and ameliorated histopathological alterations. The observed hepatoprotective effects of ESOE against Cis -induced liver injury may be attributed to its antioxidant and anti-inflammatory properties, highlighting its potential as a natural therapeutic agent in mitigating chemotherapy-associated hepatotoxicity. [Display omitted] • Ethanolic Salvia officinalis extract (ESOE) mitigates cisplatin-induced hepatotoxicity. • ESOE restores hematological parameters and hepatic enzyme levels. • ESOE attenuates oxidative stress and inflammatory markers in rat liver. • Histological examination confirms ESOE's protective effect on hepatic architecture. • ESOE suppresses caspase-3 and iNOS expression, indicating anti-apoptotic and anti-inflammatory actions. [ABSTRACT FROM AUTHOR]

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The essential oil of Salvia officinalis L. (sage) exhibits versatile biological properties. The high sensitivity of sage essential oil (SEO) to environmental conditions and limited processability represent important hurdles in its use, which, however, can be overcome by microencapsulation. The objective of the current study was to encapsulate sage essential oil into core-shell type microcapsules by complex coacervation technology and to transform it into solid form by freeze-drying. Arabic gum (GA) and three different type A gelatin (G) grades were used to investigate the effect of the gel strength on microcapsule characteristics. The formation of essential oil containing microcapsules during complex coacervation was assessed by optical microscopy while SEM imaging was used to determine the morphology of the freeze-dried forms. Characterization of microcapsules was completed with FT-IR spectroscopy. Encapsulation efficiency was determined by UV-VIS spectrophotometry and the composition of essential oil by GC-MS technique. Results revealed that by the application of selected microencapsulation technology and freeze-drying method high encapsulation efficiency values could be achieved, the gel strength of gelatin has a decisive role in microcapsule particle size and the composition of essential oil is well preserved following the technological process. [ABSTRACT FROM AUTHOR]

Enhancing the efficacy of biofertilizers and biopesticides for horticultural applications presents numerous challenges, given the need to balance effectiveness with environmental and economic factors. Achieving these goals requires rigorous research into advanced technologies and formulations capable of effectively replacing or complementing traditional chemical inputs, without compromising crop yield or quality. The present study aimed to develop a versatile and yet accessible hybrid percolation–sonication system and method, designed to optimize polyphenol extraction from nettle and sage plants. The resulting extracts were combined and applied on organic tomato crops, to evaluate their biofertilizer and biopesticide effectiveness, in comparison to conventional chemical inputs. Operating the equipment in a hybrid percolation–sonication system led to a 36% increase in polyphenols extraction for nettle and a 29% increase from sage. Regarding the effect on tomatoes, data showed that plants treated with biofertilizer extracts were over 42.88% more productive than control samples and 17.67% more productive than tomatoes treated with chemical fertilizers. Tomato plants treated with biofertilizers grew approximately 10% taller and developed stems that were 5% thicker compared to those treated with chemical fertilizers. [ABSTRACT FROM AUTHOR]

Staphylococcus aureus , particularly multi-drug resistant strains, presents significant challenges in dairy farming due to its role in causing bovine mastitis, which leads to substantial economic losses and limited treatment options. Seeking alternative therapies, we investigated the potential of a topical formulation derived from the medicinal herb Salvia officinalis to combat S. aureus growth and biofilms associated with bovine mastitis. Through systematic extraction in different solvents and fractionation by column chromatography, we isolated and identified three key multicyclic terpenoids—ferruginol, sugiol, and sclareol—exhibiting significant antimicrobial activity. The formulation effectively inhibited biofilm formation, with minimum inhibitory concentration (MIC) values ranging from 0.09 to 0.74 mg ml−1 against clinical S. aureus strains, comparable to or lower than those of the pure compounds. Moreover, it displayed robust anti-adhesive properties, reducing biofilm formation by 20%–79% at subinhibitory concentrations. Furthermore, the formulation successfully disrupted pre-existing biofilms, achieving reductions ranging from 30% to 82%. Cytotoxicity assays confirmed the safety of the formulation on mammary epithelial cells, with cell viability maintained at 100% at MIC. Our findings underscore the therapeutic potential of Sa. officinalis -derived compounds in managing bovine mastitis caused by S. aureus , emphasizing their antimicrobial efficacy and safety profile. [ABSTRACT FROM AUTHOR]

Improving the productivity of medicinal and aromatic species via eco-friendly approaches is imperative worldwide because of their therapeutic impacts. Biostimulants have been recognized among the best cultural practices in the last few decades. Among them, bee honey (BH) and ginger extract (GE) are new sources of multifunctional biostimulants that positively influence plant growth and development. However, there are currently no detailed reports on the impacts of BH or GE as promising growth and yield enhancers for medicinal and aromatic species. Also, the mechanism involved in stimulating growth and essential oil content with BH or GE is still unidentified. This work was, therefore, undertaken to analyze the impact of BH and GE on the growth, productivity, and essential oil content of sage plants (Salvia officinalis L.). Sage plants were sprayed monthly for three months with the same volume of BH (0, 5, 10, and 15 g L−1), GE (0, 5, 10, and 15 mg L−1), or tap water, which was used as a control. BH or GE application improved the plant height, branch number, herb yield, total chlorophyll content, total phenolics, and antioxidant capacity relative to the controls, more so with GE. Intriguingly, the essential oil percentage, oil yield, and oil constituents were enhanced by BH and GE. In this respect, the highest levels of biostimulants, particularly GE, were more effective. On a percentage basis, the essential oil yield per hectare was largely increased by 127.91 and 138.89% with GE (10 g L−1) in both seasons relative to the controls. The contents of IAA, GA3, and CK in THE sage leaves were substantially increased by BH and GE, and higher levels of both biostimulants and GE were more effective. The nutrient levels of N, P, K, Fe, Zn, and Mg were also elevated by BH and GE compared with the untreated plants. These results suggest that BH or GE application could be a promising biostimulant for improving the productivity of sage and provide a new understanding of their mechanisms in this aspect. [ABSTRACT FROM AUTHOR]

The role of Kongzi 孔子, in the Zhuangzi, has been a compelling story. Can we read the stories about Kongzi as constituting a type of commentary on his teachings and the early development of Confucian philosophy in general? First, let us consider the way Zhuangzi has put his own teachings into the mouth of Kongzi because he was accepted to be a sage who understood how to live well. Then, I turn to the more problematic references to Kongzi as being punished in Chapter 5 and his being a "condemned person" in chapter 6 to illustrate how these stories are taken to be forms of commentary or criticism of Kongzi's project because he did not understand complete virtue. The role of Kongzi in the Zhuangzi can be read as a criticism-like commentary using Kongzi to criticize Kongzi. [ABSTRACT FROM AUTHOR]

Removing polychlorinated biphenyls (PCBs) from the environment is an important process for the protection of biota. This work examines three different approaches to the degradation of such contaminants. The first involves the use of iron bionanoparticles (Fe-BNPs) prepared through green synthesis from selected plant matrices. The second approach entails the use of the bacteria Stenotrophomonas maltophilia (SM) and Ochrobactrum anthropi (OA) isolated from a PCB-contaminated area, Strážsky canal, located in the Slovak republic, which receives efflux of canal from Chemko Strážske plant, a former producer of PCB mixtures. The third approach combines these two methods, employing a sequential hybrid two-step application of Fe-BNPs from the plant matrix followed by the application of bacterial strains. Fe-BNPs are intended to be an eco-friendly alternative to synthetic nanoscale zero-valent iron (nZVI), which is commonly used in many environmental applications. This work also addresses the optimization parameters for using nZVI in PCB degradation, including the pH of the reaction, oxygen requirements, and dosage of nZVI. Pure standards of polyphenols (gallic acid, GA) and flavonoids (quercetin, Q) were tested to produce Fe-BNPs using green synthesis at different concentrations (0.1, 0.3, 0.5, 0.8, and 1 g.L−1) and were subsequently applied to the PCB degradation experiments. This step monitored the minimum content of bioactive substances needed for the synthesis of Fe-BNPs and their degradation effects. Experimental analysis indicated that among the selected approaches, sequential nanobiodegradation appears to be the most effective for PCB degradation, specifically the combination of Fe-BNPs from sage and bacteria SM (75% degradation of PCBs) and Fe-BNPs from GA (0.3 g.L−1) with bacteria OA (92% degradation of PCBs). [ABSTRACT FROM AUTHOR]

Biotreatment of wastewater has attracted considerable attention due to its low cost and eco-friendliness. This study developed a novel bionanocomposite comprised of Nanochitosan (NCS), Bentonite (Bt), and SnO2–ZnO nanocomposite prepared using Salvia officinalis extract as a reducing/stabilizing agent. The NCS/Bt/SnO2–ZnO bionanocomposite exhibited a rough surface with porous nature. EDX, XRD, and FTIR results confirmed the formation of the bionanocomposite. TGA indicated high thermal stability of the NCS/Bt/SnO2–ZnO. DRS results revealed that the bionanocomposite had a band gap of 2.60 eV. The surface area of NCS/Bt/SnO2–ZnO was found to be 32.54 m2 g−1 with a 17.98 nm pore size. Adsorption of Fe(III) and Pb(II) ions and photocatalytic degradation of methylene blue (MB) dye using this bionanocomposite were studied. Under optimal conditions, the highest removal of Fe(III) (99.5%) and Pb(II) (91.5%) was achieved after 5 min and 40 min, respectively. The adsorption data fitted the Langmuir isotherm and indicated the removal capacity of 555.55 and 243.90 mgg−1 for Fe(III) and Pb(II), respectively. Also, the NCS/Bt/SnO2–ZnO showed the MB dye degradation efficiency of 99% within 30 min. Therefore, the NCS/Bt/SnO2–ZnO can be used as an environmentally friendly, cost-effective, thermally stable, and high-performance adsorbent/photocatalyst to remove heavy metal ions and dye molecules. [ABSTRACT FROM AUTHOR]

Salvia officinalis L. (Lamiaceae) leaves and its essential oil is used for mouth and throat disorders, skin disorders, minor wounds, and gastrointestinal disorders, and is widely used worldwide. The research aimed to conduct a comparative study of the composition of S. officinalis essential oils from commercial samples, and their main chemotypes. The volatile constituents from S. officinalis leaves were investigated using gas chromatography (GC). The commercial samples of sage leaves were obtained from retail pharmacies in nine mainly European countries. The yield of essential oil in S. officinalis commercial leaves was between 10.0 and 24.8 mL/kg. The principal components (>5%) among the main identified 25 compounds were 1,8-cineole (8.3%–45.3%), α-thujone (3.0%–34.0%), сamphor (11.3%–29.3%), β-thujone (1.5%–12.9%), viridiflorol (1.1%–10.4%), camphene (2.6%–7.1%), and α-pinene (1.3%–5.8%). In seven (Estonia, England, France, Hungary, Belgium, Ukraine, Georgia) samples α-thujone dominated. Four samples (Estonia, Georgia, England, Hungary) belong to the most common chemotype α-thujone > camphor > 1,8-cineole. Eight chemotypes of S. officinalis essential oils have been found. Toxic thujones are widespread compounds among them. Graphic Abstract Salvia officinalis L. Leaves Samples from Different Countries" class="showdlg" rel-p="photosdshow" style="max-width:100%; margin: 0px auto; cursor: pointer" /> [ABSTRACT FROM AUTHOR]

Salvia officinalis (SO) is one of the most widely used plants in traditional medicine worldwide. In the present study, the effect of an ethanolic extract of S. officinalis leaves on hallmarks of cancer of HPV-16-positive cancer tumorigenic cells, TC-1, was analyzed in vitro. Phytochemical and spectroscopic analysis were performed. Additionally, the extract's flavonoid content, reducing iron, and antioxidant capacity were determined. In regard to the in vitro tests, the cytotoxic activity and its effect on the replicative capacity and on the cell migration of TC-1 cells were analyzed by viability and clonogenic, survival, and wound healing assays. The effect of a pre-treatment or treatment on 3D culture formation, growth, and reversion capacity was also examined. The results of the phytochemical analysis allowed the detection of tannins, saponins, steroids, and flavonoids. The flavonoids content was found to be 153.40 ± 10.68 µg/mg of extract. Additionally, the extract exhibited an antioxidant capacity and a ferric-reducing capacity of around 40% compared to the ascorbic acid. Thin layer chromatographic (TLC) analysis and spectroscopic tests showed the presence of compounds similar to quercetin and catechin flavonoids in the extract. In the in vitro assays, the SO extract induced in a concentration-dependent way changes in cell morphology, the decrease of cell viability, survival, and migration. At a concentration of 125 µg/mL, the extract inhibited spheroid formation, reduced their growth, and affected their reversion to 2D. Ethanolic extract of S. officinalis leaves had inhibitory effects on hallmarks of the cancer line HPV-16+. This suggests that the phytochemicals present in it may be a source of chemotherapeutics against cervical cancer. [ABSTRACT FROM AUTHOR]

Background & Objectives: This study was performed to evaluate the effects of peppermint and sage intercropping with application of different fertilizer resources on the dry matter yield, essential oil content, and ecological indices under drought stress. Material and Methods: A field experiment was carried out as a split split plot based on a randomized complete block design (RCBD) with 36 treatments and three replications at the faculty of Agriculture, University of Maragheh, Iran in 2019 and 2020. The main factor was three irrigation regimes included 25 (MAD25), 50 (MAD50), and 75% (MAD75) maximum allowable depletion (MAD) percentage of the soil available water (SAW) as normal irrigation, mild and severe water stress, respectively. The sub-factor was different planting patterns included peppermint monoculture, sage monoculture, and intercropping of one row peppermint + one row sage and also sub-sub factor were application of different fertilizer sources including no‐fertilizer as control, TiO2 nanoparticles (100 mg L-1), arbuscular mycorrhizal fungi (AMF) inoculation, and integrative application of AMF and TiO2 (AMF + TiO2). Results: The results showed that the dry matter yield of peppermint and sage decreased significantly by 28.7, 27% in mild stress, 55.1%, and 64.1% in severe stress, respectively. The highest dry matter yield of peppermint (144.2 g m-2) and sage (172.7 g m-2) was obtained in the monoculture with integrative application of AMF + TiO2. The maximum essential oil content of peppermint (1.51%) was observed in intercropping with integrative application of AMF + TiO2. In addition, the highest land equivalent ratio (LER), actual yield loss (AYL), and monetary indices were achieved in normal irrigation with integrative application of AMF + TiO2. Moreover, the maximum net income of two plants was recorded in intercropping with application of AMF + TiO2 under normal irrigation, which was no significant difference with integrated application of AMF + TiO2 in intercropping under mild water stress. Conclusion: Generally, it can be concluded that the integrative application of AMF + TiO2 in intercropping of peppermint/sage could be suggested as a sustainable strategy to increasing the quantity and quality of essential oil of both plants under drought stress conditions. [ABSTRACT FROM AUTHOR]

In recent years, the exploration of the therapeutic potential of Salvia has gained considerable attention, leading to a growing number of scientific studies emphasizing its pharmacological properties. Despite this, therapeutic applications of Salvia remain underexploited, requiring further investigation. Iran is a major center for sage diversity in Asia, boasting 60 Salvia species, 17 of which are unique to the area. This study aimed to comprehensively explore and compare the extracts of 102 Salvia samples belonging to 20 distinct Salvia species from Iran, providing a deeper understanding of their specific polyphenol content and, consequently, their antioxidant capabilities and potential therapeutic uses. All samples were analyzed to determine the contents of total phenolics, total flavonoids, total tannin, photosynthetic pigments, and ascorbic acid, along with their antioxidant activity. These data were then combined with the forty distinct chemical fingerprints identified by ultrafast high-pressure liquid chromatography coupled with high-resolution mass spectrometry. Multivariate data analysis was employed to find correlations and differences among the huge number of data obtained and to identify Salvia species with similar phytochemical and/or antioxidant properties. The results show that each Salvia species is characterized by a distinct class of polyphenols recognized for their antidiabetic, anti-inflammatory, cardioprotective and neuroprotective properties. Overall, our findings reveal the potential of some Salvia species for targeted therapeutic applications and provide a rational basis for the development of Salvia-derived nutraceuticals, ultimately improving the prospects for the use of Salvia in medicine. [ABSTRACT FROM AUTHOR]

The sage is a perennial medicinal and aromatic plant belonging to the Lamiaceae family. Macro-micro elements have an important place in the development of this plant. One of these elements is selenium. This study aims to examine the effect of foliar selenium (Se) application on the agronomic, biochemical, and bioactive properties of sage. The plant height (PH), fresh herb yield (FHY), dry herb yield (DHY), and dry leaf yield (DLY) as agronomic properties, and Se content in the plant (SCP), ascorbic acid content (AAC), total phenolic content (TPC), antioxidant activity (AA) and mineral composition (MC) as biochemical and bioactive properties were investigated in this context. The experiment was carried out in four replications over three years in 2018, 2019, and 2020. In the study, 0 (control), 100, 200, 400, and 800 g ha−1 Se were applied to sage leaves. At the end of the study, it was determined that Se application was effective on the agronomic characteristics of the sage, except for the first year. The application of 100 g ha−1 Se in FHY, DHY, DLY, AA, and TPC properties emerged as the recommended dose since it reached the highest values. In addition, it was evaluated that the Se application did not have a statistically significant effect on the SCP. For years with statistical significance, 400 g ha−1 Se application was recommended in terms of AAC and 100 g ha−1 Se application in terms of AA and TPC. [ABSTRACT FROM AUTHOR]

Sage (Salvia officinalis L.) is a medicinal and aromatic plant (MAP) belonging to the Lamiaceae family. Its morphological, productive and chemical characteristics are affected by abiotic and biotic factors. The use of biostimulants seems to be one of the most interesting innovative practices due to fact they can represent a promising approach for achieving sustainable and organic agriculture. Despite a large application in horticulture, the use of biostimulants on MAPs has been poorly investigated. On this basis, a field experiment in a 2-year study was done to assess the effect of foliar treatments with different types of biostimulants (containing seaweeds, fulvic acids and protein hydrolysates) and two frequencies of application on morphological, productive, and chemical characteristics of S. officinalis grown organically in Mediterranean environment. Morphological, productive, and chemical parameters were affected by the factors. The biostimulant application generated higher plant height, chlorophyll content, relative water content, biomass yield and essential oil yield compared to control plants. In addition, more frequent application of biostimulants produced higher biomass and essential oil yield. The application of fulvic acid and protein hydrolysates every week produced the highest total fresh yields (between 3.9 and 8.7 t ha-1) and total dry yields (between 1.3 and 2.5 t ha-1). The essential oil yield almost doubled (33.9 kg ha-1) with a higher frequency of protein hydrolysates application. In this study, 44 essential oil compounds were identified, and the frequency factor significantly influenced the percentage of 38 compounds. The highest percentage of some of the most representative monoterpenes, such as 1,8-cineole, a-thujone and camphor, were observed in biostimulated plants, with average increases between 6% and 35% compared to control plants. The highest values for total phenolics, rosmarinic acid, antioxidant activity were obtained in control plants and with a lower frequency of biostimulant applications. This study emphasizes how biostimulant applications may be used to improve sage production performance and essential oil parameters when produced in agricultural organic system. At the same time, biostimulants application caused a decrease in total phenolic, antioxidant activity and rosmarinic acid values. [ABSTRACT FROM AUTHOR]

Notwithstanding the fact that melatonin (MT) and titanium nanoparticles (Ti NPs) alone have been widely used recently to modulate cadmium (Cd) stress in plants, there is a gap in the comparative impacts of these materials on lowering Cd toxicity in sage plants. The objective of this study was to determine how foliar application of MT and Ti NPs affected the growth, Cd accumulation, photosynthesis, water content, lipid peroxidation, and essential oil (EO) quality and quantity of sage plants in Cd-contaminated soils. A factorial experiment was conducted using MT at 100 and 200 μM and Ti NPs at 50 and 100 mg L−1 topically, together with Cd toxicity at 10 and 20 mg Cd kg−1 soil. The results showed that Cd toxicity decreased plant growth and enhanced lipid peroxidation. The Cd stress at 20 mg kg−1 soil resulted in increases in Cd root (693%), Cd shoot (429%), electrolyte leakage (EL, 29%), malondialdehyde (MDA, 72%), shoot weight (31%), root weight (27%), chlorophyll (Chl) a + b (26%), relative water content (RWC, 23%), and EO yield (30%). The application of MT and Ti NPs controlled drought stress by reducing MDA and EL, enhancing plant weight, Chl, RWC, and EO production, and minimizing Cd accumulation in plant tissues. The predominant compounds in the EO were α-thujone, 1,8-cineole, β-thujone, camphor, and α-humulene. MT and Ti NPs caused α-thujone to rise, whereas Cd stress caused it to fall. Based on heat map analysis, MDA was the trait that was most sensitive to treatments. In summary, the research emphasizes the possibility of MT and Ti NPs, particularly MT at 200 μM, to mitigate Cd toxicity in sage plants and enhance their biochemical reactions. [ABSTRACT FROM AUTHOR]

Introduction: The present study aimed to investigate the effect of Salvia officinalis on anxiety-like behaviors triggered by intracerebroventricular injection of streptozotocin in male Wistar rats. Methods: The animals were divided into four groups: control, vehicle, streptozotocin, and streptozotocin with Salvia officinalis treatment, which included dosages of 50, 25, and 100 mg/kg. The control group received no treatment. The Vehicle group received saline intraperitoneally and intracerebroventricularly. The streptozotocin group and the streptozotocin + Salvia officinalis treatment groups received saline intraperitoneally and hydroalcoholic extract of Salvia officinalis, respectively, for 14 days. Intracerebroventricular cannulas were then implanted into the animals' brains. One week after recovery, the streptozotocin group was administered streptozotocin (3 mg/kg), while the streptozotocin+ Salvia officinalis group was administered Salvia officinalis extract intraventricularly. Anxiety-like behavior was evaluated at two time points: 5 minutes and one week after streptozotocin administration, using the Elevated Plus Maze. Results: Salvia officinalis at the high dose (100 mg/kg) significantly increased both the percentage of open arm times and the percentage of open arm entries compared to the streptozotocin group (P<0.05). The medium dose (50 mg/kg) only resulted in a significant increase in the percentage of open arm times (P<0.05), while the low dose (25 mg/kg) did not result in a reduction of streptozotocin-induced anxiety. Conclusion: The plant extract of Salvia officinalis shows a dose-dependent protective effect against anxiety induced by intracerebroventricular administration of streptozotocin in rats. [ABSTRACT FROM AUTHOR]