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Objective: The objective of this work was to understand how triglyceride plant oils can deliver strength and softness benefits to hair by their penetration. These plant oils are complex mixtures of TAGs, so the initial studies performed were with pure TAGs and then these data compared to plant oils and their measured TAG compositions. Methods: LC–MS was used to identify the di and triglycerides in coconut oil, Camellia oleifera oil and safflower seed oil. Penetration of these plant oils and pure individual triglycerides was measured by a differential extraction method. Cross‐sections of oils treated with 13C‐labelled triolein were studied by NanoSIMS to visualize location of triglyceride inside hair. Fatigue strength was measured using constant stress to generate a survival distribution. Models of the lipid‐rich cell membrane complex (CMC) were created with the equimolar ratio of 18‐methyl‐eicosanoic acid (MEAS), palmitic acid (C16:0) and oleic acid (C18:1). Results: Penetration of the individual pure TAGs was confirmed for all chain lengths and degree of unsaturation tested with higher penetration for shorter chain lengths and unsaturated fatty acids. Detailed compositional analysis of selected plant oils showed a wide variety of TAGs and penetration was also demonstrated for these oils. NanoSIMS and modelling confirmed these TAGs are penetrating the lipid‐rich CMC of hair and are interacting with the fatty acids that make up the CMC. All plant oils delivered a fatigue strength improvement by penetration into the CMC and it is proposed that these oils prevent formation and/or propagation of flaws in the CMC network that leads to breakage. Conclusions: Many plant oils with a wide range of triglyceride compositions can penetrate into hair and NanoSIMS data confirmed these oils partition into the lipid‐rich cell membrane complex. Penetration studies of individual TAGs shown to be present in these oils confirmed TAGs of varying chain length can penetrate and there is a correlation between increased penetration efficacy and shorter chain lengths and presence of unsaturation in the fatty acid chains. All the oils studied delivered single fibre fatigue strength benefits. [ABSTRACT FROM AUTHOR]

Background: Essential amino acid (EAA) and omega-3 fatty acid ingestion independently attenuate leg skeletal muscle disuse atrophy in uninjured persons. However, no data exist regarding the effectiveness of combined EAA and omega-3 fatty acid ingestion to mitigate skeletal muscle disuse atrophy in response to anterior cruciate ligament reconstruction (ACLR) surgery. This pilot trial will explore the feasibility of recruitment and retention of ACLR outpatients from a single center across 18 months to consume either a combination of omega-3 fatty acids and EAAs, or a placebo control, for 4 weeks before and 2 weeks after surgery. Methods: Thirty adult (≥ 18 years old) ACLR outpatients will be recruited for this single center, double-blind, two-arm randomized controlled feasibility pilot trial. Participants will consume either 5 g⋅day−1 of omega-3 fatty acids (fish oil) and 40 g⋅day−1 of EAAs or 5 g⋅day−1 of a control fatty acid mixture (safflower oil) and 40 g⋅day−1 of non-essential amino acids (NEAAs). Fatty acid supplements will be consumed 4 weeks before and for 2 weeks after ACLR surgery, whereas the EAAs and NEAAs will be consumed 1 week before and for 2 weeks after ACLR surgery. The primary outcomes are feasibility of recruitment and retention, with the goal to recruit 30 outpatients across 18 months and retain 22 participants upon completion of the study protocol following 12 weeks of data collection. These results will be reported using descriptive statistics, along with reasons and timepoints for study dropout. Secondary exploratory outcomes will be reported using inferential statistics for purposes of hypothesis generation and elucidation of mechanistic targets for future work; no inferences to clinical efficacy will be made. These outcomes include integrated rates of skeletal muscle protein synthesis, skeletal muscle protein content and expression of translation factors, skeletal muscle and erythrocyte phospholipid composition, and measures of skeletal muscle mass, strength, and power. Impact: This work will set the foundation for a future randomized controlled trial powered to detect an effect of EAA + omega-3 fatty acid intake on skeletal muscle size or function in response to ACLR surgery. Trial registration: ClinicalTrials.gov, NCT06233825. Registered 31 January 2024. https://clinicaltrials.gov/study/NCT06233825?term=NCT06233825&rank=1 [ABSTRACT FROM AUTHOR]

This study aimed to develop and evaluate a novel herbal gel formulation containing celecoxib and bioactive compounds of Salai Guggul for effective pain management. Celecoxib, a selective COX-2 inhibitor with anti-inflammatory and analgesic properties, was combined with menthol, safflower oil, and Salai Guggul extract to enhance therapeutic efficacy through synergistic effects. Three gel formulations (CHG I, CHG II, and CHG III) were prepared using varying concentrations of key excipients and evaluated for physicochemical properties, including pH, viscosity, extrudability, spreadability, and in vitro drug diffusion. All formulations exhibited favorable properties with pH values near skin compatibility, acceptable viscosity, and superior extrudability and spreadability. In vitro drug diffusion studies revealed that CHG I provided the highest drug release (97.27%) over 240 minutes. The study demonstrated the potential of this herbal gel formulation as a promising topical treatment for pain management, offering improved skin permeation and prolonged drug release. [ABSTRACT FROM AUTHOR]

Vegetable oils are crucial for the human diet, providing energy and essential fatty acids. This study investigates the formulation of a high-quality cold-pressed vegetable oil blend from rapeseed, sunflower, sesame, and safflower, chosen for their agronomic benefits, cost-effectiveness, and reduced environmental impact. For the first time, this study is carried out in order to enhance the nutritional profile of these blend oils compared to commercial oils. The study's results showed that all formulated blend oils had higher total polyphenol and flavonoid content. Specifically, the blend of 1/2 rapeseed, 1/4 sunflower, 1/8 sesame, and 1/8 safflower had an oil yield ranging from 37 to 39% and was rich in total polyphenols (18 mg GAE/100 g), total flavonoids (2 mg/g), antioxidant activities (52%), oleic acid (46.4%), and saturated fatty acids (11%), with a balanced omega-6/omega-3 ratio (2.5). Consuming this blend oil offers a healthier choice rich in nutrients and natural antioxidants. This could open new market opportunities and cater to the growing demand for healthier oil options, especially since it is extracted without a refining process. Further research could focus on the sensory attributes and consumer acceptance of these blend oils to ensure market success, noting that sesame and sunflower involve agreeable pronounced aromas. [ABSTRACT FROM AUTHOR]

The aim of this research was to evaluate the effects of fat type and mango peel powder (MP) on the physicochemical properties of cooked beef patties during cold storage and after in vitro digestion. Beef patties were prepared with saturated beef fat (BF) and pre‐emulsified avocado oil (AO) or pre‐emulsified safflower oil (SO). MP was added at 0% or 1%. The treatments were as follows: T1 (BF, no added MP), T2 (AO, no added MP), T3 (SO, no added MP), T4 (BF + 1%MP), T5 (AO + 1%MP), and T6 (SO + 1%MP). Substituting saturated fat with AO and SO improved the fatty acid profile of beef patties. The addition of pre‐emulsified oils increased (p < 0.05) the L∗, a∗, and b∗ values. Moreover, the incorporation of MP in the meat formulation decreased (p < 0.05) lipid oxidation during cold storage. Adding MP to the meat formulation decreased (p < 0.05) lipid oxidation before and after in vitro digestion. Replacement of saturated fat with vegetable oils and incorporation of MP may be an alternative strategy to improve the quality of beef patties during cold storage and decrease lipid oxidation after in vitro digestion. [ABSTRACT FROM AUTHOR]

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Safflower oil with high oleic is an important source of oil due to its oxidative stability and nutritional value as well as its industrial benefits. Identification of molecular markers associated with oil quality traits may aid in genotype selection in breeding efforts of safflower. To determine the genetic basis of seed oil, oleic acid, and linoleic acid content in safflower seeds, a recombinant inbred population was developed from a cross between Goldasht (low oleic line) and Mex 22–191 (high oleic line) by individual self-pollinated plants in each generation (from F2 to F9). Fatty acid components were detected by gas chromatography and subsequently analyzed by QTL analysis. The high variability in fatty acid composition was demonstrated and the first high-oleic safflower genotype was generated. A new linkage map with 69 AFLP and 45 ISSR polymorphic markers was made, covering 1472,441 cM of the safflower genome. A total of 63 individual QTLs and 238 epistatic QTLs, related to oil quality, were detected in 14 genomic regions. Some major QTLs with phenotypic variation greater than 10% were identified for the measured traits. These results demonstrate that major QTL may play an important role in increase the oleic acid of the genetic material used. Furthermore, the larger number of minor QTLs and epistatic QTLs, both of which have additive effects, indicates that the expression of these traits is also influenced by interactions between multiple genes. Our results provide additional genetic resources that can be used to precisely breed these traits in the future. [ABSTRACT FROM AUTHOR]

This study aimed to determine the effect of ultrasonic sound waves on modifying the chemical structure of biodiesel to bring its physical properties closer to petroleum diesel. In this direction, safflower oil was selected because its fatty acid composition is similar to fatty acid esters of petroleum diesel and is a sustainable source. Refined safflower oil, the free fatty acid content of which was determined, was reacted with methanol under NaOH catalyst to perform the transesterification reaction. After biodiesel production, samples were incubated in an ultrasonic bath for 60, 120, and 180 minutes. FTIR, density, free fatty acid content, flash point, viscosity, and cloud point analyses investigated the effect of incubation times on biodiesel's chemical structure and properties. FTIR spectra showed that ultrasonic sound waves partially decomposed fatty acid methyl esters and increased the number of volatile components in biodiesel. The flash point of biodiesel has been associated with a decrease of 89°C, and the low flash point is expected to increase fuel efficiency. Kinematic viscosity values were measured in the 3.4583-3.5115 mm²/s range, and density values were measured in the 0.8820-0.8872 g/ml range. These values show that biodiesel complies with national and international standards. As a result, the ultrasonic bath process applied to biodiesel showed a similar result to chemical modification methods by affecting the structure of fatty acid chains. Thus, it brought the physical properties of biodiesel closer to petroleum diesel. It is seen that this method is a more efficient alternative for biodiesel production because it does not use additional chemicals, and the process is faster. In conclusion, by increasing the production of the drought-resistant safflower plant, sustainable energy resources will be contributed, while its waste can be evaluated as animal feed. Ultrasonicated safflower biodiesel can also be used as an efficient, environmentally and mechanically friendly alternative fuel source.

Safflower is a climate-resilient, quality oilseed with high resistance to water scarcity, soil salinity, and frost-prone areas, and it has a wide range of applications in daily life, ranging from food to pharmaceutical to industrial. Adapting high-quality oil-content-producing safflower cultivars can help reduce costs and reduce precious foreign exchange in countries like Pakistan. Exotic germplasm imported from the United States Department of Agriculture-Agricultural Research Service (USDA-ARS), consisting of 145 exotic safflower accessions and four local control cultivars were planted under semi-arid conditions in Faisalabad, Pakistan, during winter 2018-19 and 2019-20 season using an augmented design with unreplicated entries and replicated checks. Genotypic coefficient of variability (GCV) analysis revealed significant variation among the accessions of safflower for achene yield plant-1, heads plant-1, and branches plant-1. The Pearson correlation analysis revealed a significant but negative correlation between days to maturity and days to 50% flowering. The results revealed larger achene yields and earlier maturity in safflower planted in early winter. Biplot analysis found that five of the tested accessions had higher achene yield plant-1, while four of the other accessions had a higher percentage of oil than the control, which was the local safflower check-31, which had the highest oil content and best quality traits. Furthermore, the dendrogram revealed that four safflower accessions exhibited higher morphological uniqueness across the investigated traits during both years of study, which can be employed for future varietal development. [ABSTRACT FROM AUTHOR]

Meat products containing Vitamin D3 (VD3) are an innovative option that could contribute to reducing deficiencies in this micronutrient. Designing nanoemulsions that carry VD3 is the first step in developing functional meat products. Thereby, this study investigated the impact of food components on the nanoemulsion properties. A central composite design was used to study the effects of pea protein (PP, 0.5–2.5%), safflower oil (SO, 5–15%), and salt (0–0.5%) on the nanoemulsion stability (ζ-potential and particle size) and the VD3 retention. Also, the optimized nanoemulsion carrying VD3 was incorporated into a meat matrix to study its retention after cooking. The combination of food components in the optimized nanoemulsion were SO = 9.12%, PP = 1.54%, and salt content = 0.4%, resulting in the predicted values of ζ-potential, particle size, and VD3 retention of −37.76 mV, 485 nm, and 55.1%, respectively. The VD3 that was nanoencapsulated and included in a meat product remained more stable after cooking than the VD3 that was not encapsulated. If a meat product is formulated with 5 or 10% safflower oil, the stability of the nanoencapsulated VD3 is reduced. This research contributes to developing functional meat products carrying nanoencapsulated vitamin D3 in natural food-grade components. [ABSTRACT FROM AUTHOR]

Biodiesel production represents a great opportunity to reduce marine and land pollution while incorporating circular economy principles into modern society. The objective of this research is to use the response surface methodology to evaluate the best engine performance and emission characteristics of safflower-based biodiesel and diesel mix dual-fuel Compression ignition engines. The optimization aims to increase brake power, and brake thermal efficiency, and reduce brake-specific fuel consumption and exhaust emissions. The independent input variables were biodiesel blending ratio, compression ratio, and engine load. The novelty of this study is to optimize the operating parameters of a dual-fuel compression ignition engine fueled with safflower biodiesel and diesel blend fraction. A total of 48 experimental trials were conducted. The oil output from safflower seed in the lab and the biodiesel yield obtained were 26% and 91%, respectively. RSM results predict the optimized magnitude of the biodiesel blending ratio, CR, and EL are B16.66, 16.68, and 12 kg, respectively. The optimal values for dependent parameters, that is, BP, BTE, BSFC, CO, UHC, CO2, and NO were 3.27 kW, 19.60%, 3.30 kg/kWh, 0.0006 (% vol.), 0.8 (ppm), 0.78 (% vol.), and 140.87 ppm, respectively. The cumulative composite desirability was found to be 0.5877. [ABSTRACT FROM AUTHOR]

Introduction Weeds are one of the most important factors that reduce the growth and yield of plants, and weed control is one of the most important managements in farms. Various methods have been reported to control weeds. In order to control weeds, farmers have to use herbicides with destructive biological effects. The use of high concentrations of herbicides increases the resistance of various weeds and endangers the health of the environment. Weeds are controlled by manual, agronomic and chemical methods. Although weed management by methods such as weeding and the use of herbicides are effective in controlling weeds, these methods are uneconomical and cause environmental pollution. Therefore, it is necessary to use an alternate method to control weeds. According to the mentioned topics, the purpose of this study is to investigate the effect of integrated and agronomic management of weeds in spring safflower fields and the yield and yield components of safflower seeds (Carthamus tinctorius L.) in Kalibar city in East Azerbaijan. Materials and methods In this study, the effect of integrated and agronomic management of weed control on the yield of safflower oil seed and medicinal plant was carried out in four replications based on a randomized complete block design. In this study, experimental treatments include the application of various weed control methods at 9 levels, including 1. Complete weeding of weeds during the entire safflower growth period (control), 2. Straw and stubble mulch of wheat (three tons/ha), 3. V. villosa (density of 50 plants per square meter), planting distance on the row 10 cm and between rows 20 cm, 4. V. ervilia cover plant (density of 50 plants per square meter), planting distance on the row 10 cm and between rows 20 cm, 5. Application of 100 % trifluralin herbicide (2.5 L/ha) before planting, 6. Application of 100% haloxyfop -- R-Methyl herbicide (1 L/ha) in the five-leaf stage of weeds and narrow leaf plants, 7. Application of 50% trifluralin herbicide (1.25 L/ha) along with straw and stubble mulch before planting, 8. Application of 50% haloxyfop -- R-Methyl herbicide (0.5 L/ha) along with straw and stubble mulch in the five-leaf stage of weeds of narrow leaf plants, 9. Lack of control of weeds. Weeds were present throughout the growth period (uncontrollable evidence). Analysis of the variance of the measured traits was done in the form of randomized complete block design with four replications. Before analysis of variance, establishing the assumptions of normality of distribution of deviations, uniformity of within-treatment variances and summability of block and treatment effects were checked and confirmed by Tukey 's test. Comparison of the average traits was done using Duncan's test at the five percent probability level. SPSS and Excel software were used for statistical analyzes and graphs. Results and discussion According to the findings of this study, the highest yield of safflower seed was achieved in the complete weeding of weeds, which was not significantly different from trifluralin herbicide + straw mulch and wheat stubble. Trifluralin herbicide treatments + straw mulch and wheat stubble and complete weeding caused an increase of 119% and 128.6% respectively in safflower seed yield. The application of mulch alone increased the yield of safflower seed by 62.5%, but the cover plant of safflower, the cover plant of cowpea and the herbicide haloxyfop -- R-Methyl had no significant effect on the yield of safflower seed. The weed control treatments increased the grain yield by increasing the number of seeds produced, but the 1000-seed weight was not affected by the weed control treatments. Conclusions The highest increase in the number of seeds per plant (34.3%), the number of seeds per plant (97.8%) and the yield of seeds (119.%) was obtained in the treatment of trifluralin herbicide + straw and stubble mulch of wheat. These results show that trifluralin herbicide + straw and stubble mulch of wheat were the most effective among the non-mechanical weed control treatments of safflower field. The results showed that the seed yield with 128.6% and the number of seeds per plant with 110% changes showed the greatest response to weed control treatments. The results showed that using straw and stubble mulch can reduce the use of chemical pesticides in weed control. According to the results of weed control, it has had a significant effect on reducing the density and biomass of weeds. Introduction Weeds are one of the most important factors that reduce the growth and yield of plants, and weed control is one of the most important managements in farms. Various methods have been reported to control weeds. In order to control weeds, farmers have to use herbicides with destructive biological effects. The use of high concentrations of herbicides increases the resistance of various weeds and endangers the health of the environment. Weeds are controlled by manual, agronomic and chemical methods. Although weed management by methods such as weeding and the use of herbicides are effective in controlling weeds, these methods are uneconomical and cause environmental pollution. Therefore, it is necessary to use an alternate method to control weeds. According to the mentioned topics, the purpose of this study is to investigate the effect of integrated and agronomic management of weeds in spring safflower fields and the yield and yield components of safflower seeds (Carthamus tinctorius L.) in Kalibar city in East Azerbaijan. Materials and methods In this study, the effect of integrated and agronomic management of weed control on the yield of safflower oil seed and medicinal plant was carried out in four replications based on a randomized complete block design. In this study, experimental treatments include the application of various weed control methods at 9 levels, including 1. Complete weeding of weeds during the entire safflower growth period (control), 2. Straw and stubble mulch of wheat (three tons/ha), 3. V. villosa (density of 50 plants per square meter), planting distance on the row 10 cm and between rows 20 cm, 4. V. ervilia cover plant (density of 50 plants per square meter), planting distance on the row 10 cm and between rows 20 cm, 5. Application of 100 % trifluralin herbicide (2.5 L/ha) before planting, 6. Application of 100% haloxyfop -- R-Methyl herbicide (1 L/ha) in the five-leaf stage of weeds and narrow leaf plants, 7. Application of 50% trifluralin herbicide (1.25 L/ha) along with straw and stubble mulch before planting, 8. Application of 50% haloxyfop -- R-Methyl herbicide (0.5 L/ha) along with straw and stubble mulch in the five-leaf stage of weeds of narrow leaf plants, 9. Lack of control of weeds. Weeds were present throughout the growth period (uncontrollable evidence). Analysis of the variance of the measured traits was done in the form of randomized complete block design with four replications. Before analysis of variance, establishing the assumptions of normality of distribution of deviations, uniformity of within-treatment variances and summability of block and treatment effects were checked and confirmed by Tukey 's test. Comparison of the average traits was done using Duncan's test at the five percent probability level. SPSS and Excel software were used for statistical analyzes and graphs. Results and discussion According to the findings of this study, the highest yield of safflower seed was achieved in the complete weeding of weeds, which was not significantly different from trifluralin herbicide + straw mulch and wheat stubble. Trifluralin herbicide treatments + straw mulch and wheat stubble and complete weeding caused an increase of 119% and 128.6% respectively in safflower seed yield. The application of mulch alone increased the yield of safflower seed by 62.5%, but the cover plant of safflower, the cover plant of cowpea and the herbicide haloxyfop -- R-Methyl had no significant effect on the yield of safflower seed. The weed control treatments increased the grain yield by increasing the number of seeds produced, but the 1000-seed weight was not affected by the weed control treatments. Conclusions The highest increase in the number of seeds per plant (34.3%), the number of seeds per plant (97.8%) and the yield of seeds (119.%) was obtained in the treatment of trifluralin herbicide + straw and stubble mulch of wheat. These results show that trifluralin herbicide + straw and stubble mulch of wheat were the most effective among the non-mechanical weed control treatments of safflower field. The results showed that the seed yield with 128.6% and the number of seeds per plant with 110% changes showed the greatest response to weed control treatments. The results showed that using straw and stubble mulch can reduce the use of chemical pesticides in weed control. According to the results of weed control, it has had a significant effect on reducing the density and biomass of weeds. [ABSTRACT FROM AUTHOR]

This study aims to improve press equipment for safflower oil production by using a mechanism that optimizes pressure distribution within screw turns. A detailed analysis of the main components of the produced safflower oil was performed, encompassing both quantitative and qualitative assessments. Through the exploration of dependencies governing the safflower oil pressing process on the screw press, the optimal parameters were determined. As a result of the research, the optimal diaphragm gap between the gape cylinder and the pressing screw was determined, with the optimal oil yield percentage achieved at ω = 6.2 rad/s and δ = 5 mm. The study also compared the performance of the existing Dream Modern ODM-01 screw press and its upgraded version by analyzing the extracted oil. The results reveal changes in the quantitative and qualitative composition of the main oil components following the operation of the existing and the modernized screw presses. For instance, the amount of unsaturated fatty acids, such as oleic acid (7.7 ± 0.566%), linoleic acid (85.3 ± 1.185%), and linolenic acid (1.2 ± 0.223%), increased. There was an increase in the presence of inorganic substances in safflower oil: iron (0.023 ± 0.031 mg/kg), phosphorus (0.086 ± 0.059 mg/kg), silicium (0.136 ± 0.075 mg/kg), and others. The findings of this study hold significant commercial value and offer promising prospects for global market implementation. [ABSTRACT FROM AUTHOR]

Safflower (Carthamus tinctorius L.) is a multipurpose minor crop consumed by developed and developing nations around the world with limited research funding and genetic resources. Genomic selection (GS) is an effective modern breeding tool that can help to fast-track the genetic diversity preserved in genebank collections to facilitate rapid and efficient germplasm improvement and variety development. In the present study, we simulated four GS strategies to compare genetic gains and inbreeding during breeding cycles in a safflower recurrent selection breeding program targeting grain yield (GY) and seed oil content (OL). We observed positive genetic gains over cycles in all four GS strategies, where the first cycle delivered the largest genetic gain. Single-trait GS strategies had the greatest gain for the target trait but had very limited genetic improvement for the other trait. Simultaneous selection for GY and OL via indices indicated higher gains for both traits than crossing between the two single-trait independent culling strategies. The multi-trait GS strategy with mating relationship control (GS_GY + OL + Rel) resulted in a lower inbreeding coefficeint but a similar gain compared to that of the GS_GY + OL (without inbreeding control) strategy after a few cycles. Our findings lay the foundation for future safflower GS breeding. [ABSTRACT FROM AUTHOR]

Safflower honey (SH) is highly valued for its unique aromatic components and potentialbiological activity. However, the current volatile profiles on SH are insufficient. Here, we characterized the pollen feature of SH and safflower pollen (SP) from pollen analysis, and performed a widely-targeted volatilome analysis on SH and SP. A total of 374 shared significantly different metabolites were identified between safflower pollen and safflower honey, including 90 terpenoids, 58 esters, 56 heterocyclic compounds, 33 alcohols and others. Of these, hotrienol was the most significantly up-regulated aroma component of SH, and therefore likely to contribute to the sweet and floral flavour. In addition, the characteristic components of safflower essential oil such as linalool, terpinen-4-ol, α-terpineol, benzaldehyde and others were detected in SH. These results suggest that safflower honey may have potential health benefits similar to those of its parent plant and could be used as a novel functional food. [ABSTRACT FROM AUTHOR]

The study aims to analyse the qualitative characteristics of safflower oil, including its fatty acid profile, and determine the acid and peroxide numbers. The study also investigated the levels of heavy metals in the oil and assessed its potential as an inhibitor of α-amylase and α-glucosidase enzymatic activity. In this study, standardised methods were used to analyse the fatty acid composition of safflower oil using a standard mixture of 37 fatty acid methyl esters (Supelco™ 37 Component FAME Mix), which ensured high accuracy in determining the fatty acid composition. The physical properties of safflower oil, such as density (0.94) and specific gravity (0.917), depend on the fatty acid composition and temperature. The refractive index, determined at 1.469, was used to estimate changes in the oil's unsaturation and viscosity (45.6 cP). Colour parameters (lightness 45.24, red-green scale -2.87, yellowblue scale 21.04) confirm the authenticity of the oil. The results of gas chromatographic analysis confirmed that the composition of the samples did not meet the requirements of the standard. The study also included the evaluation of the inhibitory activity of safflower oil against the enzymes α-amylase and α-glucosidase, where one of the samples showed significant inhibition of α-amylase activity and the other α-glucosidase activity, indicating their potential use as natural inhibitors. The safety control of heavy metal content showed that the levels of lead, cadmium and other toxic elements in the oil are well below the permissible limits, confirming its safety for consumption. These data indicate that, although some safflower oil samples do not meet the standards for fatty acid composition, its potential as a natural enzyme inhibitor and safety in terms of heavy metal content opens up prospects for further research and possible optimisation of production processes. [ABSTRACT FROM AUTHOR]

Biodiesel stands out as a promising contender in the quest for renewable energy solutions, offering a greener alternative to traditional fossil fuels. Derived primarily from the transesterification of vegetable oils or animal fats, biodiesel offers an eco-friendly energy avenue with a minimized carbon footprint. Catalysts are central to the success of this process, which significantly enhance yield rates. Geopolymers, traditionally associated with construction applications due to their inorganic nature, have been derived from aluminosilicate sources activated using alkaline solutions. However, recent advancements spotlight geopolymers in a new light, emphasizing their prospective role as nanocatalytic agents for biodiesel synthesis. This paradigm shift suggests improved production efficiency and an innovative method of repurposing industrial waste. This study centers on the pioneering application of geopolymers, fortified with magnetite, as potent heterogeneous catalysts for biodiesel generation from soybean and safflower oils. By leveraging a meticulously crafted geopolymer matrix—consisting of metakaolin, sodium hydroxide, and magnetite—this research replaced traditional catalysts with this advanced nanostructured geopolymer variant in the biodiesel methylation process. The research delved deep to ascertain the prime synthesis conditions. Furthermore, utilizing cutting-edge machine learning methodologies provided an analytical lens to navigate the extensive experimental data, thereby fine-tuning the optimization trajectory. One of the salient takeaways from this research is the validation that geopolymer catalysts, rooted in kaolinite, can be ingeniously tailored to ensure elevated biodiesel yields across a spectrum of oil sources, underscoring their unparalleled efficiency and versatility in the biofuel domain. [ABSTRACT FROM AUTHOR]

Oil droplets of S/O/W emulsion were manipulated by magnetic field. This S/O/W emulsion is safflower oil, containing magnetic particles, dispersed in water. This S/O/W emulsion is prepared using SPG membrane. First, magnetic field were applied to the statically placed S/O/W emulsion by an electromagnet from the side. As a result, the oil droplets moved in the direction of the magnet. Then, the magnetic field were applied to the oil droplets of the S/O/W emulsion flowing in a bifurcated channel branching in two directions. As a result, more than half of the oil droplets were guided to one of the branches. These experiments showed the larger the diameter of the oil droplets and the stronger the magnetic field, the faster the oil droplets moved. The larger the diameter of the oil droplet, the more it was guided to one of the branches. [ABSTRACT FROM AUTHOR]

This research letter explores the potential therapeutic benefits of safflower oil-based linoleic acid (LA) supplementation on Barth syndrome cardiomyopathy. Barth syndrome is a lipid disorder caused by mutations in the TAFAZZIN gene, and cardiomyopathy is a significant clinical feature of the syndrome. The study investigates the effects of a high-LA safflower oil diet on mouse models of Barth syndrome and finds that LA supplementation can prevent and partially reverse cardiomyopathy in these models. The findings suggest that LA supplementation could be a potential treatment for Barth syndrome cardiomyopathy, although more research is needed to understand its long-term effects and mechanisms of action. [Extracted from the article]

In this investigation, the examination revolves around the characterization of diatomite-enhanced modified safflower oil (MSO)-derived polyester biocomposites. The primary objective is to explore the feasibility of these biocomposites as a substitute for petrochemical-based unsaturated polyester (UP) materials, with the overarching goal of enhancing their economic sustainability. Experimental data analysis employed Response Surface Methodology (RSM) and Artificial Neural Network (ANN), uncovering the optimal composition for the polyester biocomposite to be 6.7 wt.% MSO and 4.5 wt.% diatomite. During the RSM analysis, it was noted that the response parameters exhibited quadratic p -values, specifically, for density (p <.0001), thermal conductivity (p <.0001), and Shore D hardness (p <.0003). However, higher ratios of MSO lead to decreased hardness and increased curing time. SEM images reveal a detrimental impact on the surface morphology of the polyester biocomposite when the diatomite content reaches 8 wt.%. Additionally, Fourier Transform Infrared Spectroscopy (FTIR) and Thermogravimetric Analysis (TGA) offer valuable insights into the chemical bond structure and thermal behavior of the biocomposite, respectively. The Cure Index (CI) value for the diatomite-enhanced composite was determined to be 0.925, indicating a favorable contribution to the polyester curing process. The study finds that diatomite contributes to a linear change in the thermal conductivity coefficient, making the biocomposite suitable for use in the insulation industry. Overall, the study suggests that diatomite reinforced MSO-based polyester biocomposites have the potential as an alternative to petrochemical unsaturated polyester. [ABSTRACT FROM AUTHOR]

Induction of obesity by dietary fats and oils differs according to the type of fat. Adiponectin is believed to be related to obesity prevention. We hypothesized that flaxseed oil is important for preventing obesity and producing adiponectin. To clarify this hypothesis, we investigated the relationship between obesity and different fat sources in mice fed diets with 6 types of fat and oils. C57BL/6J mice were given a control diet containing 5% corn oil or a high-fat diet containing 20% of either lard, palm oil, rapeseed oil, oleate-rich safflower oil, corn oil, or flaxseed oil for 14 weeks. In another experiment, mice were given a control diet and rosiglitazone (10 mg/kg body weight) by oral gavage for 1 week. At the end of study, plasma adiponectin and expression of fatty acid metabolism–related factors in white and brown adipose tissue and the liver were measured. Dietary flaxseed oil, which is rich in α-linolenic acid, did not induce obesity. Flaxseed oil resulted in increased β-oxidation–related factors in epididymal white adipose tissue, decreased fatty acid synthesis–related factors in the liver, and thermogenesis-related factor in brown adipose tissue following increase of plasma adiponectin. The results suggested that increase in plasma adiponectin after intake of flaxseed oil may be due to altered expression of AdipoQ and peroxisome proliferator-activated receptor γ in epididymal white adipose tissue. Flaxseed oil increased expression of adiponectin in visceral fat and regulated obesity-controlling fatty acid metabolism-related factors in white adipose tissue and liver, and thermogenesis-related factor in brown adipose tissue. A high-fat diet containing lard or palm oil induced obesity, whereas a high-fat diet containing flaxseed oil prevented it. Flaxseed oil promoted mRNA expression of AdipoQ in eWAT, but not in sWAT. Plasma adiponectin induced by flaxseed oil related to regulation of β-oxidation–related factors in eWAT, fatty acid synthesis–related factors in the liver, and thermogenesis-related factor in BAT. Abbreviations: BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; sWAT, subcutaneous white adipose tissue. [Display omitted] [ABSTRACT FROM AUTHOR]

In this study, we examined the effects of seven different sulfur treatments on safflower seeds. The treatments included: no sulfur application (S0), 25 kg/ha of pure bulk sulfur (S25), 50 kg/ha of pure bulk sulfur (S50), 25 kg/ha of sulfur phosphate (Sp25), 50 kg/ha of sulfur phosphate (Sp50), 25 kg/ha of zinc sulfate (Zs25), and 50 kg/ha of zinc sulfate (Zs50). Our evaluation covered various seed quality attributes, including ash percentage (ASH), oil percentage (OIL), and protein percentage (PRO). Additionally, we analyzed the fatty acid composition, including palmitic acid 16: 0 (PAL), stearic acid 18: 0 (STE), oleic acid 18: 1 (OLE), linoleic acid 18: 2 (LINL), arachidic acid 20: 0 (ARA), and linolenic acid 18: 3 (LINN). The vector-view of the biplot illustrated positive associations among the fatty acids STE, PAL, and OLE, whereas ASH exhibited negative associations with OIL, LINL, and LINN. The polygon-view graph was divided into four sectors, with the genotype S50 emerging as the top performer for attributes such as OIL, PRO, LINL, ARA, and LINN. Treatment Zs50 occupied the vertex of another sector and displayed the highest values for palmitic acid PAL, STE, and OLE, while treatment S0 was positioned at the vertex of the next sector, characterized by its high ASH content. By utilizing the ideal tester tool of treatment by trait biplot, we identified OIL as the desirable trait that most effectively represented the data. The qualitative properties of safflower oil were notably influenced by sulfur application, with treatment S50 proving to be the most effective in enhancing these properties. [ABSTRACT FROM AUTHOR]

The combined effect of γ-linolenic acid (GLA)-rich evening primrose oil and soybean phospholipid on lipid metabolism was investigated. Male ICR mice were fed diets containing 100 g/kg of coconut, safflower or GLA oil, supplemented with 31 g/kg of soybean oil or 50 g/kg of soybean phospholipid, for 21 d. All experimental diets provided the same amount of fatty acids. The diets containing GLA oil significantly reduced hepatic triacylglycerol levels compared with those containing coconut oil. The values obtained from diets containing GLA oil also tended to be lower than those obtained from the diets containing safflower oil. Soybean phospholipid, regardless of the type of dietary fat, reduced hepatic triacylglycerol levels. Therefore, the combination of GLA oil and soybean phospholipid was effective in causing a marked decrease in hepatic triacylglycerol levels. Changes in hepatic fatty acid oxidation and lipogenesis may be responsible for the observed results. [ABSTRACT FROM AUTHOR]

Background: Safflower (Carthamus tinctorius L.) is an oilseed crop with substantial medicinal and economic value. However, the methods for constructing safflower core germplasm resources are limited, and the molecular mechanisms of lipid biosynthesis in safflower seeds are not well understood. Results: In this study, 11 oil-related quantitative traits and 50 pairs of InDel markers were used to assess the diversity of a collection of 605 safflower germplasms. The original safflower germplasm exhibited rich phenotypic diversity, with high variation for most of the phenotypic traits under investigation. Similarly, high genetic diversity was evaluated in the original germplasm, in which the mean Shannon's information index (I), observed heterozygosity (H0), and expected heterozygosity (He) were 0.553, 0.182, and 0.374, respectively. Four subgroups with strong genetic structures were identified and a core germplasm of 214 cultivars was constructed, which is well represented in the original germplasm. Meanwhile, differential expression analysis of the transcriptomes of high and low linoleic acid safflower varieties at two stages of seed development identified a total of 47 genes associated with lipid biosynthesis. High expression of the genes KAS II and SAD enhanced the synthesis and accumulation of oleic acid, while FAD genes like FAD2 (Chr8G0104100), FAD3, FAD7 and FAD8 promoted the consumption of oleic acid conversion. The coordinated regulation of these multiple genes ensures the high accumulation of oleic acid in safflower seed oil. Conclusions: Based on these findings, a core germplasm of 214 cultivars was constructed and 47 candidate genes related to unsaturated fatty acid biosynthesis and lipid accumulation were identified. These results not only provide guidance for further studies to elucidate the molecular basis of oil lipid accumulation in safflower seeds, but also contribute to safflower cultivar improvements. [ABSTRACT FROM AUTHOR]

Oil cakes are pomace that is a by-product of pressing oil from oilseeds. Due to the content of bioactive ingredients, there is more and more talk about their valorization through use in human and animal nutrition as well as microbiological processes aimed at the biosynthesis of biosurfactants, enzymes and fragrance compounds. The aim of this research was a qualitative assessment of selected oil cakes (linseed, rapeseed, hemp, safflower and camelina oil cakes) in the context of their potential use in microbiological cultures aimed at the biosynthesis of green note aroma compounds. The scope of the research included the analysis of the fatty acid composition of the lipid fraction of oil cakes as well as the determination of lipoxygenase activity and the polyphenol content in the extracts of these raw materials. Chromatographic analysis of the lipid fraction showed that hemp and safflower cakes had the highest content of polyunsaturated fatty acids. PUFAs constitute 67.18 ± 2.2% and 73.72 ± 1.8% in their extracts, respectively. Hemp cake extracts were also characterized by the highest lipoxygenase activity—76.4–1.2 U/mL with a low content of phenolic compounds at the level of 0.116 ± 0.005 mg GAE/mL of extract. [ABSTRACT FROM AUTHOR]

The antioxidant activity of curcumin (0.02-0.1%) was evaluated in olive, sesame, and safflower oils at 373, 383, and 393 K. The results were examined in contrast to the effects of tocopherol (0.1%) and BHT (0.02%), so that the inhibitory function of curcumin was evaluated comparatively. The activation energy of oxidation was determined for olive (82.94 kJ·mol-1), sesame (77.39 kJ·mol-1) and safflower oils (74.42 kJ·mol-1). Adding curcumin (0.1%) enhanced the activation energy by 26.26, 26.64, and 38.81% in the case of olive, safflower, and sesame oils, respectively. Based on Gibbs free energy, curcumin functioned more effectively in olive oil at 373 K (growth coefficient: 1.52%), compared to the action of the other two antioxidants, namely tocopherol (1.43%) and BHT (1.39%). The efficiency of curcumin was lower in oils which had a higher degree of polyunsaturation due to the disproportionation of the hydrogen-donating mechanism and the rate of free-radical formation in these oils. [ABSTRACT FROM AUTHOR]

The objective of the present study was to increase the frying stability of refined safflower oil (RSO) by blending it with refined olive pomace oil (ROPO) during deep fat frying. For this purpose; RSO, ROPO and their blends were utilized for frying of potato sticks at 180°C for 3 consecutive days. The frying stability of the oils was monitored by analyzing them for their free fatty acids, peroxide values, total polar contents, ultraviolet spectrophotometric indices at 232 and 270 nm, fatty acid profiles, p-anisidine values, α- tocopherol contents and photometric color indices. 3-monochloropropane-1,2-diol (3-MCPD) and glycidyl ester (GE) levels of oils before and after frying were measured as well. The results have shown that thermooxidative degradation products increased as the frying progressed for all oils, however the decomposition rate was found to slow down in blend oils by stabilizing with ROPO. Blending RSO with ROPO decreased linoleic and linolenic; but increased the oleic and palmitic acid percentages of the blends. C18:2/C16:0 ratio was found to decrease by frying for RSO and the blend oils, however ROPO was not affected significantly. 3-MCPD-E levels of the blends increased as the ratio of ROPO increased. Principal component analysis enabled a clear discrimination between oils with different composition throughout the frying process. [ABSTRACT FROM AUTHOR]

Conjugated linoleic acid (CLA) is perceived to protect the body from metabolic diseases. This study was conducted to determine the effect of Lactiplantibacillus plantarum (Lp. plantarum) on CLA production and sensory characteristics of cheddar cheese. Lp. plantarum can convert linoleic acid (LA) to CLA. To increase CLA in cheddar cheese and monitor the conversion of LA to CLA by Lp. plantarum, the LA content of cheese milk (3.4% fat) was increased by partially replacing fat with safflower oil (85% LA of oil) at 0, 3, 6, and 9% concentrations (T1, T2, T3, and T4). Furthermore, Lp. plantarum 108 colony-forming units (CFU)/mL (8 log CFU mL−1) was added in all treatments along with traditional cheddar cheese culture (Lactococcus lactis ssp. lactis and L. lactis ssp. cremoris). After 30 days of ripening, Lp. plantarum in T1, T2, T3, and T4 was 6.75, 6.72, 6.65, and 6.55 log CFU g−1. After 60 days of ripening, Lp. plantarum in T1, T2, T3, and T4 was 6.35, 6.27, 6.19, and 6.32 log CFU g−1. After 60 days of ripening, Lp. plantarum in T1, T2, T3, and T4 was 6.41, 6.25, 6.69, and 6.65 log CFU g−1. GC-MS analysis showed that concentrations of CLA in the 90 days' control, T1, T2, T3, and T4 were 1.18, 2.73, 4.44, 6.24, and 9.57 mg/100 g, respectively. HPLC analysis revealed that treatments containing Lp. plantarum and LA presented higher concentrations of organic acids than the control sample. The addition of safflower oil at all concentrations did not affect cheese composition, free fatty acids (FFA), and the peroxide value (POV) of cheddar cheese. Color flavor and texture scores of experimental cheeses were not different from the control cheese. It was concluded that Lp. plantarum and safflower oil can be used to increase CLA production in cheddar cheese. [ABSTRACT FROM AUTHOR]

This study aims to improve press equipment for safflower oil production by using a mechanism that optimizes pressure distribution within screw turns. A detailed analysis of the main components of the produced safflower oil was performed, encompassing both quantitative and qualitative assessments. Through the exploration of dependencies governing the safflower oil pressing process on the screw press, the optimal parameters were determined. As a result of the research, the optimal diaphragm gap between the gape cylinder and the pressing screw was determined, with the optimal oil yield percentage achieved at ω = 6.2 rad/s and δ = 5 mm. The study also compared the performance of the existing Dream Modern ODM-01 screw press and its upgraded version by analyzing the extracted oil. The results reveal changes in the quantitative and qualitative composition of the main oil components following the operation of the existing and the modernized screw presses. For instance, the amount of unsaturated fatty acids, such as oleic acid (7.7 ± 0.566%), linoleic acid (85.3 ± 1.185%), and linolenic acid (1.2 ± 0.223%), increased. There was an increase in the presence of inorganic substances in safflower oil: iron (0.023 ± 0.031 mg/kg), phosphorus (0.086 ± 0.059 mg/kg), silicium (0.136 ± 0.075 mg/kg), and others. The findings of this study hold significant commercial value and offer promising prospects for global market implementation.

In this study, safflower oil oleogels were made using propolis wax and carnauba wax in three different concentrations each, and their effectiveness as a fat substitute in cake was evaluated afterward. Oleogels' oxidative stability and characterization were looked into. In oleogels, the oil binding capacity, solid fat content, and crystallization time were all assessed. The cakes underwent an examination for moisture content, texture, and sensory evaluation. Additionally, analyses of fatty acid composition, free fatty acidity, oxidative stability (peroxide value, conjugated diene‐triene), 3‐monochloropropane‐1,2‐diol (3‐MCPD), and glycidyl were carried out both before and after baking in oleogels and shortening. Several of the physical, textural, and sensory qualities of the oleogel‐based cakes were acceptable when compared to those of the shortening‐based cakes. The general acceptability of cakes made with carnauba wax was very high and almost under control. The acceptability of cakes made with propolis wax oleogels was lower than this. The study of these criteria has shown that safflower oil‐based carnauba and propolis wax oleogels can be utilized to produce high‐quality, healthful cakes with a high amount of unsaturated fatty acids. Practical Applications: To replace fat phases in cake products high in saturated fatty acids and to enhance the fatty acid profile of the cakes, safflower oil‐based oleogels with propolis wax and carnauba wax are a very good option. The results obtained provide useful information for the production of high‐quality cakes with higher unsaturated fatty acid content, recommended for a healthier diet, with these oleogels containing different concentrations of oleogelator. [ABSTRACT FROM AUTHOR]

There is growing interest in exploring new types of cultivated plants that offer unique health benefits. Safflower is a promising plant nutrition resource that has gained popularity in Kazakhstan and worldwide. However, one of the pressing issues is improving the consumption pattern of food products, including vitamins and microelements. This article aims to develop a small‐scale processing line for safflower oil production in small enterprises of the cereal‐processing industry. The primary focus is on purifying safflower from impurities and husks, as well as compressing and centrifuging the oil raw material for high‐quality and biologically valuable product. The authors developed a processing line consisting of a sheller‐compress and a centrifuge of original constructions for separating husks from the kernel and compressing and centrifuging the oil raw material. The process combines husking, compressing, filtration, and sedimentation to ensure primary purification of plant oils and the removal of mechanical impurities. The production of safflower oil has become an urgent task due to its rich composition of vitamins and phospholipids. The newly developed processing line ensures the production of high‐quality safflower oil and has great potential for small enterprises in the cereal‐processing industry. The article highlights the importance of primary purification of plant oils and its impact on the quality of the final product. Practical applications: The following conclusions emerged from the research. The relevance of fat oil growing, including signs of increased popularity of safflower oil in Kazakhstan and in the world has been studied. Various types (husking, compressing, sedimentation, centrifuging and filtering) of primary purification of safflower and purification of oil from mechanical impurities have been investigated. The dependence of extraction of pressed safflower oil on the speed of rotation of the filter and settling rotor and the equipment structure itself has been studied. The sheller‐compress for purification of safflower has been developed to combine husking and compressing processes to increase the oil content of the processed oil raw material and the productivity of the processing equipment. The centrifuge is designed for primary purification of safflower oil from mechanical impurities, allowing to combine the sedimentation and filtering processes due to rotors and to obtain purified safflower oil. The problem of intensification of the purification process is characterized by the ratio of different defined parameters. As a result, optimum parameters were determined and a processing line was developed with the use of a sheller‐compress and a centrifuge with a filtering and pre. [ABSTRACT FROM AUTHOR]

This study aimed to determine the chemical properties (fatty acid composition, oil content, sterol and tocopherol compositions) of the oils extracted from the seeds of safflower (Dinçer, Remzibey, Balci, Linas, Yenice, Olas) varieties harvested in different periods from flowering to ripening period. In parallel with the increase of harvest time, the humidity rate decreased, while the oil ratios increased. It was determined that palmitic (16:0) and stearic (18:0) acids, which are significant saturated fatty acids, and oleic (18:1) and linoleic (18:2) acids, which are unsaturated fatty acids, are quite high in the oils of all safflower varieties. These fatty acids showed significant changes from the first harvest to the last harvest. The total saturated fatty acid ratios decreased, while the amount of unsaturated fatty acids increased as the maturation progressed. The first and latest harvest samples of Dinçer, Remzibey, Balcı, Linas, Yenice, Olas cultivars were selected and their sterol and tocopherol compositions were examined. The highest level of sterol in all cultivars was β‐sitosterol and the amount of sterols decreased towards full maturity. It was determined that α‐tocopherol was the dominant tocopherol found in the safflower oils and the amount of tocopherol increased towards full maturity. [ABSTRACT FROM AUTHOR]

Safflower oil is a very valuable product for the body and human health. It is rich in macro- and microelements, vitamins and minerals, and also has antioxidant properties. The primary purification of safflower oil is an important stage of its production and directly affects the quality of the final product and its storage ability. Purifying safflower oil using a combination of filtration and sedimentation processes in an experimental cone-shaped centrifuge is a new direction in its processing. The purpose of this study was to determine the effects of flax fiber as a filter material for safflower oil. The Akmai variety of the safflower was tested. The results showed that the quality indicators of safflower oil before and after filtration through flax fiber are different. The amount of unsaturated fatty acids such as oleic (18.31 ± 0.874%) and cis-linoleic acid (82.52 ± 1.854%) increased, as well as the content of arginine (2.1), tyrosine (0.57), methionine (0.4), cystine (2.5), tryptophan (2.6), and other amino acids (in oil g per 100 g of protein). The increase in the total amount of phenols (322.12 ± 6 mgEAG/kg of oil) was observed, which directly caused the higher antioxidant activity (42.65 ± 8%) of the safflower oil. These results demonstrate that flax fiber can enrich safflower oil. To find the optimal conditions for safflower oil centrifugation in a cone-shaped sedimentary-filtering centrifuge, the thickness of the flax fiber and the distance between the inner and outer perforated filter rotor were tested. It was found that the optimal and effective thickness of the flax fiber is 1.5 × 107 nm, while the thickness of the sediment is 0.5 × 107 nm. [ABSTRACT FROM AUTHOR]

Wei safflower seed oil (WSO) prepared by the cold pressing method and organic solvent extraction method was characterized in this study. The yield of cold-pressed WSO (CP-WSO) was inferior to that of n-hexane-extracted WSO (HE-WSO). The physicochemical properties (refractive index, density, iodine value, insoluble impurities) and fatty acid compositions were similar, and both were rich in linoleic acid. However, CP-WSO had better color and less solvent residue. The type and content of vitamin E in CP-WSO was also superior to that in HE-WSO, which explained the high oxidative stability of CP-WSO in the Rancimat test. Our results provide a reference for the development of Wei safflower seed oil. [ABSTRACT FROM AUTHOR]

Hexanal, hexenal, nonenal and their corresponding alcohols are used as green notes in the fragrance and flavour industry. The production of bio-based hexanal starts from linoleic acid, which can be obtained from sunflower or safflower oil. The biocatalytic process utilizes C13-specific lipoxygenase (LOX) for hydroperoxidation and consecutive splitting with hydroperoxide lyase (HPL). In this study, we investigated the chemical splitting of the LOX product 13-HPODE in comparison to HPL catalysis. In addition, 13-HPODE was synthesized using enriched linoleic acid from safflower oil. Varying amounts of soybean flour suspension as a source of LOX yielded up to 60% HPODE with a regioselectivity of 92% towards 13-HPODE. Using low-toxicity Lewis acids like AlCl3 and ZrCl4, cleavage of the produced 13-HPODE was possible. A maximum hexanal yield of 22.9% was reached with AlCl3 under mild reaction conditions, though product degradation was an interfering process. Comparative trials with N-terminal truncated HPL from papaya revealed hexanal recovery within a comparable range. Additionally, we successfully demonstrated the viability of Hock rearrangement of 13-HPODE through heterogeneous catalysts. Notably, Beta zeolite and Montmorillonite K10 exhibited a turnover frequency (TOF) on par with common heterogeneous catalysts employed in industrial processes. [ABSTRACT FROM AUTHOR]

Safflower oil‐based oleogels were produced from beeswax and rice bran wax. Oleogels demonstrated higher oxidative stability than shortening at the cooking temperature. Peroxide values in shortening, rice bran wax oleogels, and beeswax oleogels samples were found in the range of 4.8–27.76, 13.21–20.45 and 4.30–7.72 meqO2kg−1 oil. Following oleogelation, there was no significant change in fatty acid composition of safflower oil. In addition, after baking process, the changes in the major fatty acids were not determined to be significant. Solid fat content ratios (carried out at 35°C) of rice bran wax oleogels, in beeswax oleogels and in shortening samples were defined in the range of 4.10%–7.70%, 0.80%–5.00%, and 9.61%, respectively. The highest oil binding capacity was revealed in beeswax oleogels with 99.93%–99.98%. The shortest crystallization time was determined as 3 min in oleogel containing 10% rice bran wax. Cakes consisted of oleogel were acceptable in terms of texture and sensory properties compared to cake produced with shortening. Sensory results revealed that some cakes produced with oleogels were found to be more acceptable as compared with control group samples. In this respect, oleogels produced with safflower oil‐based beeswax and rice bran wax could be used instead of commercial solid fat widely used in the cake industry. [ABSTRACT FROM AUTHOR]

Oxidative stress and inflammation, as natural parts of metabolic adaptations during the transition from late gestation to early lactation, are critical indicators of dairy cows' metabolic health. This study was designed to investigate the effects of abomasal infusion of essential fatty acids (EFA), particularly α-linolenic acid, and conjugated linoleic acid (CLA) on plasma, erythrocyte, and liver markers of oxidative stress in dairy cows during the transition period. Rumen-cannulated German Holstein cows (n = 38) in their second lactation (11,101 ± 1,118 kg milk/305 d, mean ± standard deviation) were abomasally infused with one of the following treatments from d −63 antepartum until d 63 postpartum (PP): CTRL (n = 9; 76 g/d coconut oil); EFA (n = 9; 78 g/d linseed plus 4 g/d safflower oil); CLA (n = 10; isomers cis -9, trans -11 and trans -10, cis -12 CLA; 38 g/d); and EFA+CLA (n = 10; 120 g/d). Hematological parameters as well as markers of oxidative status were measured in plasma, erythrocytes, and liver before and after calving. Immunohematological parameters, including erythrocyte number, hematocrit, hemoglobin, mean corpuscular hemoglobin, leukocytes, and basophils, were affected by time, and their peak levels were observed on the day after calving. The oxidative stress markers glutathione peroxidase 1 and reactive oxygen metabolites in plasma and erythrocytes were both affected by time, exhibiting the highest levels on d 1 PP, whereas β-carotene, retinol, and tocopherol were at their lowest levels at the same time. Immunohematological parameters were only marginally affected by fatty acid treatment in a time-dependent manner. As such, lymphocyte and atypical lymphocyte counts were both significantly highest in the groups that received EFA at d 1 PP. Moreover, EFA supplementation increased the mean corpuscular volume and showed a trend for induction of mean corpuscular hemoglobin compared with the CLA group during the transition period. The PP mean thrombocyte volume was higher in the EFA than in the CLA group (except for d 28) and both EFA and CLA reduced number of thrombocytes and thrombocrit at distinct time points. Hepatic mRNA abundance of markers related to oxidative status, including glutathione peroxidase (GPX-1) and catalase (CAT), was lower (P < 0.05) in EFA-treated than non-EFA-treated cows at d 28 PP. Dairy cows at the onset of lactation were characterized by induced markers of both oxidative stress and inflammation. Supplementing EFA and CLA had minor and time-dependent effects on markers of oxidative stress in plasma, erythrocytes, and liver. A comparison of EFA supplementation with CLA or CTRL showed higher immunohematological response at d 1 PP and lower hepatic antioxidant levels by d 28 PP. Supplementation with EFA+CLA had only a minor effect on oxidative markers, which were more similar to those with the EFA treatment. Altogether, despite the time-dependent differences, the current findings show only minor effects of EFA and CLA supplementation in the prevention of early lactation-induced oxidative stress. [ABSTRACT FROM AUTHOR]

Investigated were the influence of drying and pressing parameters of rapeseed, sunflower, and safflower seeds on oil yield, as well as antioxidant addition on the oxidative stability of cold pressed oils. The parameters of the oilseeds and cold pressed oils quality (moisture content, iodine value, saponification value, peroxide value, free fatty acids, and insoluble impurities content) were determined using standard methods. In terms of oxidative stability, rosemary, green tea, and pomegranate extracts were used as natural antioxidants, and propyl gallate was used as synthetic antioxidant. The results showed that the moisture content of the oilseeds, as well as nozzle size and electromotor frequency of the press had an effect on the yield of cold pressed oil. By reducing the nozzle size and electromotor frequency, the volume of produced cold pressed oil increased. Rosemary extract effectively increased the oxidative stability of sunflower and safflower oils, and green tea extract increased the stability of rapeseed oil. Greater stabilisation of the tested oils was achieved with the addition of 0.4 % of natural antioxidants compared to the addition of 0.2 %. The synthetic antioxidant, propyl gallate, failed to stabilise safflower oil, and unlike the natural antioxidants, it was less protective for rapeseed and sunflower oil. [ABSTRACT FROM AUTHOR]

To increase the nutritional value of wheat bread as a staple food using non-conventional wholesome floury ingredients should meet high expectations of consumers. The study was aimed to investigate the effect of amaranth groats application into breadmaking focused on the technological qualities and biological value of wheat bread enriched with onion powder and safflower oil. The influence of scalded amaranth groats on the bread quality was measured by technological, physical, chemical, instrumental, and computational methods analysing raw materials and developed products. The use of the amaranth grain-derived ingredient at 4-8% as a valuable plant source in the wheat bread formulations enhanced the product consumer characteristics. The proofing of dough of the developed formulations was intensified followed by 6-20% increase in the bread specific volume and better organoleptic properties of bread. Amaranth groats showed high amino acid score for lysine (156%), phenylalanine and tyrosine (125%), and scores for threonine, valine, and cysteine were 2-fold to wheat flour scarce in bioactive compounds. The developed wheat-amaranth bread had an improved amino acid composition due to an increase in the number of essential amino acids, a 2.6 times higher protein utility, contributing to its digestibility. The main factor in changing the fatty acid composition of the developed products was the introduction of safflower oil with a high content of linoleic acid into the product. Introduction of scalded amaranth groats into bread formulations with vegetable-based additives is promising way to attribute the product with therapeutic, and health-improving properties

Objective. The intent of the present study was to test two hypotheses. The primary hypothesis was that there would be differences between blood serum individual free fatty acids (SIFFA) and serum individual total fatty acids (SITFA) in terms of their different relationships (correlations) to each of homeostatic model assessment-individual insulin resistance (HOMA-IR) and homeostatic model assessment-individual insulin resistance-percentage β-cell function (HOMA-% β) remaining in human type 2 diabetic patients with pre-flaxseed oil (FXO) and pre-safflower oil (SFO) administration. The secondary hypothesis was that FXO (rich in alpha-linolenic acid, ALA) supplementation would alter these correlations differently in the SIFFA and STIFFA pools in comparison with the placebo SFO (poor in ALA).