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Your search keyword '"Saeki, Masachika"' showing total 28 results
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28 results on '"Saeki, Masachika"'

3. Analytical performance of four rapid molecular testing for SARS-CoV-2

Author

Katayama Yuki, Murai Ryosei, Sato Yuki, Moriai Mikako, Nirasawa Shinya, Saeki Masachika, Yakuwa Yuki, Fujiya Yoshihiro, Kuronuma Koji, and Takahashi Satoshi

Subjects
rapid molecular test, sars-cov-2, sensitivity, Medical technology, R855-855.5
Abstract

Various reagents and equipment for testing SARS-CoV-2 infections have been developed, particularly rapid molecular tests based on polymerase chain reaction (PCR).

Published
2022
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5. Clinical performance and potential of a SARS-CoV-2 detection kit without RNA purification steps

Author

Sato Yuki, Kondo Takashi, Moriai Mikako, Nirasawa Shinya, Saeki Masachika, Yakuwa Yuki, Katayama Yuki, Kitayama Ikumi, Kobayashi Ryo, Endoh Akemi, Asanuma Koichi, Fujiya Yoshihiro, and Takahashi Satoshi

Subjects
assay, covid-19, detection, real-time rt-pcr, sars-cov-2, Medical technology, R855-855.5
Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is rapidly spreading globally. Early diagnosis plays an essential role in controlling the infection. Therefore, early and accurate SARS-CoV-2 detection assays along with easy operation are required. The aim of this study was to compare the clinical performance of the Ampdirect™ 2019-nCoV Detection Kit (SHIMADZU assay), which does not require RNA purification steps, with that of the preexisting SARS-CoV-2 detection assays, which use a purified RNA template.

Published
2021
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15. Diagnostic importance of overexpression of Bmi-1 mRNA in early breast cancers.

Author

Saeki M, Kobayashi D, Tsuji N, Kuribayashi K, and Watanabe N

Subjects
Female, Humans, Immunohistochemistry, Nuclear Proteins biosynthesis, Polycomb Repressive Complex 1, Proto-Oncogene Proteins biosynthesis, RNA, Messenger analysis, ROC Curve, Repressor Proteins biosynthesis, Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity, Biomarkers, Tumor analysis, Breast Neoplasms genetics, Breast Neoplasms pathology, Gene Expression, Nuclear Proteins genetics, Proto-Oncogene Proteins genetics, Repressor Proteins genetics
Abstract

Target molecules for a highly sensitive and specific diagnosis of breast cancer in its early clinical stages have not yet been identified. Here, we show the first evidence for diagnostic performance of the molecule B cell-specific Moloney murine leukemia virus integration site 1 (Bmi-1) in breast cancer patients. Only 5 out of 46 non-cancerous samples were positive for Bmi-1 mRNA expression resulting in a sensitivity and specificity of 72.0 and 91.3%, respectively. The mRNA expression was estimated using the cut-off value obtained from the receiver operating characteristic curve analysis. Further, Bmi-1 mRNA expression was found to be elevated in 97.8% (45/46) of cancerous tissues in comparison to the expression in paired cancerous tissues and non-cancerous tissues obtained from identical patients. Bmi-1 mRNA was found to be highly expressed even in the early clinical stages of breast cancer. Our results suggest that Bmi-1 mRNA might be a new tool to support the diagnosis of breast cancers, irrespective of the clinical stage.

Published
2009
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