Search

Your search keyword '"Saegusa M"' showing total 328 results
Start Over Author "Saegusa M"
328 results on '"Saegusa M"'

5. Loss of DCC expression in astrocytomas: relation to p53 abnormalities, cell kinetics, and survival. (papers)

Author

Saegusa, M, Mikami, T, Okayasu, I, and Hara, A

Subjects
Astrocytoma -- Genetic aspects -- Prognosis, Tumor suppressor genes -- Analysis -- Genetic aspects, Colorectal cancer -- Prognosis -- Genetic aspects, Gliomas -- Genetic aspects -- Prognosis, Health, Analysis, Genetic aspects, Prognosis
Abstract

Abstract Aims--Although frequent reduction or loss of DCC (deleted in colorectal carcinomas) has been demonstrated in gliomas, the association with cell kinetics and survival is still unclear. Methods--A total of [...]

Published
2001

6. S100A16, a promising candidate as a prognostic marker for platinum-based adjuvant chemotherapy in resected lung adenocarcinoma

Author

Katono K, Sato Y, Kobayashi M, Nagashio R, Ryuge S, Igawa S, Ichinoe M, Murakumo Y, Saegusa M, and Masuda N

Subjects
S100A16, Lung adenocarcinoma, Prognostic marker, Platinum-based adjuvant chemotherapy, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Immunohistochemistry, lcsh:RC254-282
Abstract

Ken Katono,1 Yuichi Sato,2 Makoto Kobayashi,3 Ryo Nagashio,2 Shinichiro Ryuge,1 Satoshi Igawa,1 Masaaki Ichinoe,4 Yoshiki Murakumo,4 Makoto Saegusa,4 Noriyuki Masuda1 1Department of Respiratory Medicine, School of Medicine, 2Department of Molecular Diagnostics, School of Allied Health Sciences, 3Department of Applied Tumor Pathology, Graduate School of Medical Sciences, 4Department of Pathology, School of Medicine, Kitasato University, Minami-ku, Sagamihara, Kanagawa, Japan Purpose: Although cisplatin-based adjuvant chemotherapy improves the survival of patients with resected non-small-cell lung cancer, not all patients show a survival benefit, and some patients experience severe toxicity. Therefore, identifying biomarkers is important for selecting subgroups of patients who may show improved survival with platinum-based adjuvant chemotherapy. S100A16 is thought to play key roles during different steps of tumor progression. The aim of this study was to evaluate the use of S100A16 expression as a prognostic marker in patients with completely resected lung adenocarcinoma receiving platinum-based adjuvant chemotherapy. Methods: S100A16 expression was immunohistochemically studied in 65 consecutive lung adenocarcinoma patients who underwent complete resection and received platinum-based adjuvant chemotherapy. Kaplan–Meier survival analysis and Cox proportional hazards models were used to estimate the effect of S100A16 expression on disease-free survival (DFS) and overall survival (OS).Results: S100A16 expression was detected in 26 of the 65 (40.0%) lung adenocarcinoma patients. Although S100A16 expression was not correlated with DFS (P=0.062), it was significantly correlated with OS (P=0.009). In addition, multivariable analysis revealed that S100A16 expression independently predicted a poorer survival (HR =4.79; 95% CI =1.87–12.23; P=0.001). Conclusion: The present study revealed that S100A16 is a promising candidate as a prognostic marker for platinum-based adjuvant chemotherapy in resected lung adenocarcinoma. A further large-scale study is needed to confirm the present results. Keywords: S100A16, lung adenocarcinoma, platinum-based adjuvant chemotherapy, immunohistochemistry, prognostic marker

Published
2017

18. General Lectures-(II)

Author

Toriie, S., Nakajima, M., Kohli, Y., Takebayashi, M., Misaki, F., Kobayashi, A., Hashimoto, Y., Mitsuyoshi, Y., Ida, K., Kawai, K., Fujita, R., Kumura, F., Takahashi, M., Ohsawa, H., Hasegawa, Y., Kidokoro, T., Jojima, Y., Miyoshi, H., Okumura, Y., Yokouchi, A., Asano, H., Uehara, A., Oka, S., Saito, H., Yaginuma, M., Yamamoto, T., Asano, A., Nakamura, M., Okada, Y., Tomioka, T., Tamesue, N., Ikeda, S., Kunisaki, T., Emura, T., Yasumoto, M., Someya, N., Goto, A., Morokuma, K., Sakamoto, H., Kasaki, Y., Imamura, K., Amano, I., Iwanaga, K., Okugawa, N., Ninomiya, K., Yoshida, S., Okabe, N., Shimogawa, Y., Nakajima M., Ida, K., Akasaka, Y., Shimamoto, K., Misaki, F., Toriie, S., Tada, M., Sugawara, K., Chikayasu, I., Takeda, H., Kawai, K., Shibue, T., Yamaguchi, A., Osame, T., Shimada, K., Hori, M., Irisa, T., Miura, Y., Hanamure, B., Chuman, Y., Sato, H., Kizu, M., Kasugai, T., Kuno, N., Aoki, I., Nitta, Y., Machii, A., Murakami, Y., Aiso, Y., Kitahara, N., Yoshizawa, Y., Hishinuma, Y., Nao, Y., Ishiyama, K., Watanabe, M., Kimura, T., Hara, T., Sakaue, S., Shinoda, T., Ito, R., Hayashi, K., Sugie, H., Sekizawa, H., Nakamura, S., Kondo, J., Tanaka, E., Taniguchi, T., Kanayama, M., Koizumi, S., Watabiki, S., Ikeda, S., Tamura, K., Okada, Y., Arai, T., Saito, T., Tojo, S., Furuya, M., Hanaue, H., Ogoshi, K., Murohisa, B., Uchimura, M., Ishigaki, J., Waki, S., Nakafuji, H., Iida, F., Aratake, K., Nakano, T., Ono, N., Hara, I., Sassa, T., Takahashi, T., Inoue, J., Maruyama, Y., Mori, Y., Kawamura, T., Imai, F., Yorita, S., Saegusa, M., Ishihara, F., Asukata, I., Matsuda, M., Wakabayashi, K., Mitani, S., Sugahara, K., Ishikawa, K., Nakayoshi, A., Nakamoto, M., Kono, A., Oda, T., Sato, Y., Takahashi, T., Nagao, F., Nagano, M., Furusawa, T., Nakama, T., Itoh, H., Nishimura, M., Tsutsumi, K., Saito, H., Yoshida, K., Ito, H., Yoshimura, M., Yoshioka, K., Shimizu, T., Kuroda, C., Uchida, H., Ishida, O., Inoue, T., Hasegawa, S., Mitsusada, K., Kajiyama, Y., Matsuzawa, Y., Yokota, K., Hayashida, Y., Ikegudhi, S., Shida, S., Tsurumi, K., Ito, K., Kamiya, K., Takada, H., Ueno, S., Onda, M., Kojima, G., Shoji, E., Miyaji, M., Goto, K., Nitta, Y., Yazaki, Y., Ito, M., Kozuka, M., Tanabe, A., Murate, H., Takeuchi, T., Koshikawa, M., Kato, N., Maeda, K., Hayashi, K., Tsuru, T., Ooyama, I., Kukimoto, H., Nakashima, Y., Katsuki, T., Ueda, N., Kanno, T., Noda, A., Toda, Y., Hayakawa, T., Nakajima, S., Morishita, R., Furukawa, K., Ikeda, F., Fujii, M., Yamamoto, T., Wakisaka, G., Matasumoto, Y., Ono, H., Hirose, S., Kobayashi, K., Sawabu, N., Takeuchi, J., Kajikawa, K., Takada, A., Hirai, Y., Ohki, I., Sato, K., Tasaka, S., Sato, A., Aono, G., Kidokoro, T., Takezoe, K., Jojima, Y., Ohara, T., Soma, S., Ukawa, S., Yamaguchi, S., Shirahama, T., Sugiyama, K., Koyama, H., Haga, M., Arikawa, H., Toyokawa, H., Sato, R., Ueno, Y., Karasawa, Y., Onuma, H., Suzuki, H., Murakami, T., Yasui, A., Ichinose, Y., Hirase, Y., Okazaki, T., Takai, T., Watanabe, K., Kato, M., Yamada, M., Tsuji, T., Kunito, Y., Kobayashi, S., Udo, K., Iriyama, K., Sugiura, Y., Takahashi, Y., Kawamura, O., Ando, S., Hayashi, K., Tsuji, K., Yukawa, Y., Saito, N., Miyazawa, M., Imai, K., Tabayashi, T., Ito, H., Umehara, S., Watanabe, Y., Murai, S., Nukaga, A., Ishimatsu, N., Sotoyama, S., Abe, M., Hirai, K., Yoshida, K., Aoki, T., Nagao, F., Aoki, Y., Taniguchi, K., Wada, N., Tabuse, K., Yanagi, I., Tsuhada, K., Katsumi, M., Nakamura, K., Takemoto, K., Yamaura, Y., Karibe, N., Yamada, G., Ichikawa, H., Ogiwara, M., Matsushita, M., Kobayashi, I., Kusano, M., Yasuna, O., Hayashi, S., Yoshizumi, M., Kojima, Y., Matsubayashi, K., Yamamoto, T., Nishimura, R., Koga, M., Tachibana, M., Kurata, M., Suto, H., Ichinose, I., Ishizuka, K., Shimoda, M., Onai, M., Akiyama, T., Sekiguchi, T., Kobayashi, S., Matsuyoshi, M., Yonezawa, N., Kasamatsu, M., Yokota, Y., Toyoda, T., Uchimoto, I., Kanamoto, M., Fukai, Y., Sugawara, K., Katoh, M., Takebayashi, M., Mitsuyoshi, Y., Shimamoto, K., Nakamura, I., Tamura, M., Nishio, M., Mukaide, Y., Kuzumoto, Y., Ota, K., Yoshida, T., Sakamoto, A., Akiyama, T., Kaneko, S., Yanagida, M., Kishimoto, S., Miyaji, K., Shiraki, Y., Inoue, K., Tamada, T., Usui, T., Ohtsuka, K., Yamada, S., Fujishima, S., Tamiya, A., Saji, K., Ueda, A., Shiraki, Y., Inoue, K., Tamada, T., Usui, T., Ohtsuka, K., Yamada, S., Fujishima, S., Tamiya, A., Saji, K., Ueda, A., Yasutake, K., Irie, K., Ijiri, Y., Sato, H., Nishijima, H., Ogino, K., Okuno, T., Date, H., Yao, T., Koga, Y., Tomioka, T., Fuyuno, S., Okabe, H., Mitsui, H., Tamechika, Y., Masuda, N., Fujiwara, T., Sakimura, M., Okada, Y., Takamura, Y., Kono, T., Kurihara, M., Sumida, M., Izumi, T., Haraikawa, M., Hayakawa, H., Shirakabe, H., Yasui, A., Ushio, K., Okamoto, M., Noguchi, M., Kinoshita, A., Yamada, T., Ichikawa, H., Shimotori, K., Kudo, T., Mukaida, I., Ishikawa, H., Sato, K., Shirane, T., Kano, A., Suzuki, T., Tanaka, M., Iwanaga, T., Taniguchi, H., Inawashiro, M., Endo, N., Kawamura, T., Suzuki, Sh., Suzuki, H., Maki, T., Hayakawa, K., Ikezawa, H., Jao, C. C., Yamada, K., Nakamura, M., Tanaka, M., Maruyama, M., Nagasako, K., Oi, I., Kozu, T., Yamashita, K., Yokoyama, I., Endo, M., Takemoto, T., Nakayama, K., Hayakawa, R., Ishiguro, M., Nakano, H., Nakazawa, S., Tsuboi, Y., Yamase, H., Yamashita, T., Fujita, T., Ishikawa, Y., Ito, N., Mitsuno, T., Kanazawa, K., Yamashiro, M., Kubo, T., Iizuka, H., Watanabe, T., Sanada, M., Satoh, H., Shimada, H., Kusama, S., Ishikawa, K., Ikeda, K., Naramoto, J., Koga, M., Okazaki, Y., Nakamura, K., Kawamura, S., Fujimoto, S., Urayama, S., Matsuura, H., Sekitani, T., Sasayama, T., Nakagawa, K., Toyama, K., Nakagawa, S., Takada, T., Kusaka, K., Takaba, S., Satomura, A., Suzuki, T., Kawakami, Y., Watanabe, Y., Suzuki, H., Koike, T., Joh, S., Hara, T., Tamura, K., Kobayagawa, K., Hashimoto, T., Uemura, F., Fukui, O., Takahashi, M., Takasato, Y., Shirakawa, K., Hisamatsu, M., Saito, T., Ashizawa, S., Sakurane, Y., Miyamura, K., Sasaki, H., Katsumi, M., Ura, S., Emoto, M., Tatsumi, Y., Totsuka, Y., Chiba, N., Ozeki, M., Nakazawa, M., Takamura, S., Iida, F., Sawano, Y., Sugita, T., Funabiki, T., Watanabe, S., Moriya, T., Tomita, N., Nishida, K., Todo, A., Miyake, T., Suzaki, Y., Yamamoto, Y., Ariyoshi, J., Hajiro, K., Oishi, M., Yanagihara, K., Nakamura, A., Kuramata, H., Soeda, S., Kondo, N., Akashi, M., Hemmi, T., Kadono, H., Ito, T., Tsuchiya, R., Ikeda, Y., Futatsuki, K., Nomoto, S., Kino, I., Arai, M., Shimazu, H., Kobori, O., Ishikawa, K., Hiroshima, Y., Matsusaka, Y., Katase, K., Sakuma, Y., Murata, N., Komura, K., Ando, H., Ohara, K., Hayashi, A., Suzuki, M., Watanuki, T., Asano, T., Koide, N., Shiobara, M., Matsuo, N., Imai, K., Segawa, K., Nakazawa, S., Okabe, N., Kawai, K., Matsumoto, K., Shimada, H., Machida, K., Koizumi, Y., Hoshi, Y., Oi, M., Watanuki, T., Seki, H., Matsumura, M., Kimura, M., Yoshimura, M., Ishikawa, I., Kishi, S., Kondo, Y., Uchida, Y., Harada, H., Mandai, M., Kikuchi, T., Mishima, K., Yamagata, Y., Suyama, T., Kawagoe, K., Inagawa, T., Kishimoto, S., Sugiya, T., Kai, T., Miyoshi, A., Fukumoto, S., Watanabe, K., Kojo, H., Turuhara, I., Miyoshi, Y., Okamoto, K., Inata, H., Okamoto, H., Sakurai, S., Sugiyama, M., Sasaki, H., Miura, K., Kurihara, T., Sakumoto, K., Okita, E., Tanaka, H., Ishihara, K., Okawa, M., Okumura, Y., Watanabe, Y., Nishizaki, N., Murakami, T., Saito, K., Aoyagi, K., Hamaguchi, E., Kitamura, T., Matsuo, Y., Seki, A., Mori, H., Ishikawa, T., Nakajima, T., Shimomura, T., Sengoku, K., Aoyagi, K., Hamaguchi, E., Uchiya, Y., Yabana, T., Konta, M., Kamijo, K., Yachi, A., Okuse, S., Ohara, H., Sato, K., Wada, T., Kurata, M., Furuta, K., Nishii, M., Yamawaki, T., Nishii, K., Umeda, K., Yoshida, H., Ito, H., Okabayashi, T., Kato, Y., Yatsuji, Y., Suzuki, Y., Nomura, K., Matsumoto, K., Kamisaka, K., Motoki, T., Kamii, K., Kameda, H., Imamura, H., Uchiya, T., Ishizawa, M., Nishizaki, H., Murakami, T., Orimo, H., Yoshida, A., Yoshida, A., Orimo, H., Fujita, T., Oda, T., Itoh, Z., Honda, R., Takeuchi, S., Fukushima, T., Suda, T., Shinonaga, M., Ishiguro, N., Fujisawa, S., Nishiyama, K., and Ohkubo, T.

Published
1973
Full Text
View/download PDF

19. Functional role of ALK-related signal cascades on modulation of epithelialmesenchymal transition and apoptosis in uterine carcinosarcoma.

Author

Inoue, H., Hashimura, M., Akiya, M., Chiba, R., and Saegusa, M.

Subjects
APOPTOSIS, UTERINE diseases, UTERINE cancer, GYNECOLOGIC cancer, CANCER cells, CANCER treatment
Abstract

Background: Anaplastic lymphoma kinase (ALK), which is a receptor tyrosine kinase, is essentially and transiently expressed in the developing nervous system. Recently, the deregulated expression of full-length ALK has been observed in some primary solid tumors, but little is known about its involvement in the tumorigenesis of uterine carcinosarcomas (UCSs). Here we examined the functional role of the ALK gene in UCSs. Methods: Regulation and function of the ALK gene were assessed using two endometrial carcinoma cell lines. Expression of ALK and its related molecules were also investigated using clinical samples of UCSs. Results: In cell lines, ALK promoter activity was significantly increased by transfection of Sox11 and N-myc, which are known to contribute to neuronal properties. Cells stably overexpressing full-length ALK showed an enhancement of EMT properties mediated by TGF-β1 and HGF, along with an increase in phosphorylated (p) Akt and nuclear p65. Overexpression of p65 also led to transactivation of Twist1 gene, known as an EMT inducer. Finally, treatment of the stable ALK-overexpressing cells with doxorubicin resulted in inhibition of apoptosis with progressive increase in the expression ratio of both pAkt and bcl2 relative to total Akt and bax, respectively. In clinical samples, strong cytoplasmic ALK immunoreactivity and mRNA signals without rearrangement or amplification of the ALK locus were frequently observed in UCSs, particularly in the sarcomatous components. Further, ALK IHC score was found to be positively correlated with Sox11, N-myc, Twist1, and bcl2 scores. Conclusion: ALK-related signal cascades containing Akt, NF-κB, Twist1, and bcl2 may participate in initial signaling for divergent sarcomatous differentiation driven from carcinomatous components in UCSs through induction of the EMT process and inhibition of apoptotic features. [ABSTRACT FROM AUTHOR]

Published
2017
Full Text
View/download PDF

20. Intra-articular fracture of the knee with spondyloepiphyseal dysplasia congenita: successful result of open reduction and internal fixation

Author

Kozo Nakamura, Takashi Matsushita, Saegusa M, Hiromi Oda, and K. Inokuchi

Subjects
musculoskeletal diseases, Spondyloepiphyseal dysplasia, Adult, Joint Instability, medicine.medical_specialty, medicine.medical_treatment, Knee Injuries, Osteochondrodysplasias, medicine, Tibial plateau fracture, Internal fixation, Humans, Orthopedics and Sports Medicine, Kyphosis, Kyphoscoliosis, Femur fracture, Osteosynthesis, business.industry, General Medicine, musculoskeletal system, medicine.disease, Surgery, Tibial Fractures, Atlanto-Axial Joint, Scoliosis, Atlantoaxial instability, Spondyloepiphyseal dysplasia congenita, Female, business, Femoral Fractures
Abstract

In spondyloepiphyseal dysplasia congenita (SEDC), since the cartilage is congenitally abnormal, functional recovery of an intra-articular fracture is uncertain even with surgical treatment. We report a 29-year-old Japanese woman with SEDC whose left knee injury (intercondylar femur fracture and tibial plateau fracture) was surgically reduced and fixed. Although special care was required during the operation for associated atlantoaxial instability and cardiopulmonary suppression due to severe thoracolumbar kyphoscoliosis as well as osteopenia, she had neither restriction of knee motion nor pain at follow-up 2 years and 4 months after surgery. Therefore, although the situation involving fractures in a patient with SEDC is complicated, we believe the main problem to be solved is whether the risk-related kyphoscoliosis and atlantoaxial instability can be managed or not. Fractures themselves can be treated based on the principles used for patients without SEDC.

Published
2001

35. Recent results of LH experiments on the JT-60 tokamak

Author

Akaoka, N, primary, Akasaka, H, additional, Akiba, M, additional, Akino, N, additional, Ando, T, additional, Annou, K, additional, Aoyagi, T, additional, Arai, T, additional, Arakawa, K, additional, Araki, M, additional, Azumi, M, additional, Chiba, S, additional, Dairaku, M, additional, Ebisawa, N, additional, Fujii, T, additional, Fukuda, T, additional, Funahashi, A, additional, Furukawa, H, additional, Gunji, H, additional, Hamamatsu, K, additional, Hanada, M, additional, Hara, M, additional, Haraguchi, K, additional, Hiratsuka, H, additional, Hirayama, T, additional, Hiroki, S, additional, Hiruta, K, additional, Honda, M, additional, Horiike, H, additional, Hosogane, N, additional, Iida, Y, additional, Iijima, T, additional, Ikeda, K, additional, Ikeda, Y, additional, Imai, T, additional, Inoue, T, additional, Isaji, N, additional, Isaka, M, additional, Isei, N, additional, Ishida, S, additional, Itami, K, additional, Itige, N, additional, Ito, T, additional, Kakizaki, T, additional, Kamada, Y, additional, Kaminaga, A, additional, Kaneko, T, additional, Kawai, M, additional, Kawabe, M, additional, Kawamata, Y, additional, Kawano, Y, additional, Kikuchi, K, additional, Kikuchi, M, additional, Kimura, H, additional, Kimura, T, additional, Kishimoto, H, additional, Kitamura, S, additional, Kiyono, K, additional, Kodama, K, additional, Koide, Y, additional, Koide, T, additional, Kobayashi, T, additional, Komata, M, additional, Kondo, I, additional, Kondo, T, additional, Konoshima, S, additional, Kubo, H, additional, Kunieda, S, additional, Kurihara, K, additional, Kuriyama, M, additional, Kusaka, M, additional, Kusama, Y, additional, Kushima, T, additional, Maeno, M, additional, Matoba, T, additional, Matsuda, S, additional, Matsukawa, M, additional, Matsuoka, M, additional, Matsuzaki, Y, additional, Miura, Y, additional, Miya, N, additional, Miyachi, K, additional, Miyake, K, additional, Miyo, Y, additional, Mizuno, M, additional, Mogaki, K, additional, Moriyama, S, additional, Murakami, Y, additional, Muto, M, additional, Nagami, M, additional, Nagashima, A, additional, Nagashima, K, additional, Nagashima, T, additional, Nagaya, S, additional, Nagayama, K, additional, Naito, O, additional, Nakamura, H, additional, Nagafuji, T, additional, Nemoto, H, additional, Nemoto, M, additional, Neyatani, Y, additional, Ninomiya, H, additional, Nishino, N, additional, Nishitani, T, additional, Nobusaka, H, additional, Nomata, H, additional, Oikawa, A, additional, Obara, K, additional, Odajima, K, additional, Ogiwara, N, additional, Ohga, T, additional, Ohara, Y, additional, Oohara, H, additional, Ohshima, T, additional, Ohta, K, additional, Ohta, M, additional, Ohuchi, S, additional, Ohuchi, Y, additional, Okumura, H, additional, Omori, K, additional, Omori, S, additional, Omori, Y, additional, Ozeki, T, additional, Saegusa, M, additional, Saitoh, N, additional, Sakasai, A, additional, Sakata, S, additional, Sakuma, T, additional, Sasajima, T, additional, Satou, K, additional, Satou, M, additional, Sawahata, M, additional, Seimiya, M, additional, Seki, M, additional, Seki, S, additional, Shibanuma, K, additional, Shimada, M, additional, Shimizu, K, additional, Shimizu, M, additional, Shimomura, Y, additional, Shinozaki, S, additional, Shirai, H, additional, Shirakata, H, additional, Shitomi, M, additional, Suganuma, K, additional, Sugawara, T, additional, Sugie, T, additional, Sunaoshi, H, additional, Suzuki, M, additional, Suzuki, N, additional, Suzuki, S, additional, Tachibana, H, additional, Takahashi, M, additional, Takahashi, S, additional, Takahashi, T, additional, Takasaki, M, additional, Takatsu, H, additional, Takeuchi, H, additional, Takeshita, A, additional, Takizuka, T, additional, Tamura, S, additional, Tanaka, S, additional, Tanaka, T, additional, Tanaka, Y, additional, Tani, T, additional, Terakado, M, additional, Terakado, T, additional, Tobita, K, additional, Totsuka, T, additional, Toyoshima, N, additional, Tsugita, T, additional, Tsuji, S, additional, Tsukahara, Y, additional, Tsuneoka, M, additional, Uehara, K, additional, Uramoto, Y, additional, Usami, H, additional, Ushigusa, K, additional, Usui, K, additional, Yagyu, J, additional, Yamagishi, K, additional, Yamagiwa, M, additional, Yamamoto, M, additional, Yamashita, O, additional, Yamazaki, T, additional, Yokokura, K, additional, Yokoyama, K, additional, Yoshida, H, additional, Yoshida, Z, additional, Yoshino, R, additional, Yoshioka, Y, additional, Yonekawa, I, additional, and Watanabe, K, additional

Published
1990
Full Text
View/download PDF

37. DCC expression is related to mucinous differentiation but not changes in expression of p21WAF1/Cip1 and p27Kip1, apoptosis, cell proliferation and human papillomavirus infection in uterine cervical adenocarcinomas.

Author

Saegusa, M and Okayasu, I

Subjects
*ADENOCARCINOMA, *UTERINE cervix incompetence, *MUCINS
Abstract

To clarify possible roles of DCC expression in tumour differentiation and cell kinetics, we immunohistochemically investigated 80 uterine cervical adenocarcinomas (C-ACs), including 31 mucinous (M) and 31 endometrioid (E) lesions, and 18 adenocarcinomas in situ (AIS), along with 39 normal cervical samples. The results were compared with findings for p21[SUPWAF1/Cip1] and p27[SUPKip1] expression, apoptosis, cell proliferation and human papillomavirus (HPV) infection. Nine C-AC cases were also examined using a combination of the reverse transcription-polymerase chain reaction and Southern blot hybridization, as well as Western blot assays. Significantly decreased DCC scores were observed in E-ACs but not M-ACs, as compared to normal cervical glandular epithelia and AIS. Average p21[SUPWAF1/Cip1] and p27[SUPKip1] scores were significantly higher in E-ACs than M-ACs, in line with high apoptotic, mitotic and Ki-67 labelling indices. A concordance of the results for DCC and p21[SUPWAF1/Cip1] expression between mRNA- and protein-based assays was also noted. Change of DCC expression, however, was not related to any of the cell kinetic markers or clinicopathological features in ACs of either type. There was also no association with the HPV status, although infection was significantly linked with high values for cell kinetics. These results suggest that DCC expression in C-ACs is closely associated with mucinous differentiation. [ABSTRACT FROM AUTHOR]

Published
1999
Full Text
View/download PDF

38. The role of the Epstein-Barr virus in the oncogenesis of EBV(+) gastric carcinomas.

Author

Takano, Y., Kato, Y., Saegusa, Makoto, Mori, Shigeo, Shiota, Mami, Masuda, Mitsunobu, Mikami, Tetsuo, Okayasu, Isao, Saegusa, M, Mori, S, Shiota, M, Masuda, M, Mikami, T, and Okayasu, I

Abstract

Five hundred and thirteen cases of gastric carcinoma were investigated for the presence of viral RNA, and the clinico-pathological data, geno-type, BamHIF restriction fragment polymorphism (RFLP) and specific LMP-1 30 bp gene deletion were also examined. EBVs detected in lymphocytes in 20 normal gastric mucosa, 7 lymphoma cell lines (LCLs) maintained in severe combined immunodeficiency (SCID) mice and 18 non-Hodgkin's lymphomas were compared with those in the gastric carcinoma cases. Thirty-three cases (6.4%) were demonstrated to be positive for EBV by means of EBER-1 RNA in situ hybridization. Clinico-pathological data showed no statistically significant difference in histological grading, location of cancer and status of vessel and lymphatic invasion between the EBV-positive and -negative groups, although the former significantly predominated in the submucosal invasion group (submucosal vs mucosal P=0.021; submucosal vs advanced cancer P=0.033). Some of these data were different from corresponding data in earlier reports. In cases that were evaluated by molecular biology, type A, wild-type F and LMP-1 gene deletion predominated except one in 21 informative cases, one in 24 and two in 16, respectively. EBVs detected in lymphocytes in normal gastric mucosa, LCLs in SCID mice and non-Hodgkin's lymphoma were also predominantly affected by type A, wild-type F and LMP-1 gene deletion with few exceptions. The results indicate a lack of genetic differences among EBVs in gastric carcinoma, normal population, LCLs of SCID mice and non-Hodgkin lymphomas. Some EBV infections in gastric carcinomas may be transient, especially in the submucosal invasion group. [ABSTRACT FROM AUTHOR]

Published
1999
Full Text
View/download PDF

39. Bcl-2 expression and allelic loss of the p53 gene in gastric carcinomas.

Author

Saegusa, M., Takano, Y., Kamata, Y., and Okayasu, I.

Abstract

In order to clarify the association between bcl-2 protein (Bcl-2) expression and genetic alteration, we investigated p53 and DCC (deleted in the colon carcinoma gene locus) gene abnormalities in Bcl-2-positive and-negative gastric carcinomas using a polymerase chain reaction/loss of heterozygosity (LOH) assay. Bcl-2 immunoreactivity was found in 25 of 178 (14%) gastric carcinoma cases. With these 25 positive cases, the proportion 18/87 (20.6%) of the total in early stages demonstrating invasion of the mucosa and/or submucosa was significantly greater ( P=0.013) than the 7/91 (7.7%) found for advanced tumors exhibiting invasion into or through the muscularis propria. However, there was no statistically significant variation between the proportions for differentiated (17/98 cases, 17.3%) and undifferentiated (8/80 cases, 10%) lesions. Sixteen Bcl-2-positive cases (9 cases were not studied because of insufficient specimen material to allow extraction of DNA) and 31 cases randomly selected from a Bcl-2-negative group were analyzed for the presence of p53-LOH or DCC-LOH and for p53 by immunohistochemistry. The minority of the Bcl-2-positive group had p53-LOH and were immunopositive for p53 ( P=0.033, P=0.028 respectively), while no association was found in the Bcl-2-negative category. In contrast, there was no correlation at all between Bcl-2 expression and DCC-LOH although the number of informative cases analyzed was too small to allow definite conclusions. These results indicate that Bcl-2 may be predominantly expressed at an early stage in gastric carcinomas, possibly in negative association with p53 gene abnormalities. [ABSTRACT FROM AUTHOR]

Published
1996
Full Text
View/download PDF

44. Significant correlations of E-cadherin, catenin, and CD44 variant form expression with carcinoma cell differentiation and prognosis of extrahepatic bile duct carcinomas.

Author

Mikami, T, Saegusa, M, Mitomi, H, Yanagisawa, N, Ichinoe, M, and Okayasu, I

Abstract

To clarify the relation between alteration of expression of cell adhesion molecules and progression of extrahepatic bile duct carcinomas. 55 cases were immunohistochemically examined for E-cadherin, alpha-catenin, beta-catenin, and CD44, with additional reverse transcription-polymerase chain reaction and Southern blotting hybridization (RT-PCR/SBH) assays. Levels of E-cadherin, alpha-catenin, and beta-catenin proteins were lower in carcinomas than in normal mucosa, while CD44 variants 3 and 6 were upregulated. Well-differentiated carcinoma showed higher expression of E-cadherin and alpha-catenin than moderately to poorly differentiated types. Macroscopically papillary lesions had higher expression of E-cadherin than their nonpapillary counterparts. RT-PCR/SBH for CD44 revealed the CD44 variant form to be more prevalent in carcinoma than in normal mucosa, correlating with the immunohistochemical results, and with more exon variety. The Cox proportional hazards test identified histologic type and E-cadherin expression as prognostic factors. Among the examined molecules, E-cadherin was especially related to papillary mass formation and a good prognosis.

Published
2001
Full Text
View/download PDF

45. Recent results in JT-60 experiments

Author

Nagami, M, primary, Aoki, I, additional, Akaoka, N, additional, Akasaka, H, additional, Akiba, M, additional, Akino, N, additional, Ando, T, additional, Annou, K, additional, Aoyagi, T, additional, Arai, T, additional, Arakawa, K, additional, Araki, M, additional, Azumi, M, additional, Chiba, S, additional, Dairaku, M, additional, Ebisawa, N, additional, Fujii, T, additional, Fukuda, T, additional, Funahashi, A, additional, Furukawa, H, additional, Gunji, H, additional, Hamamatsu, K, additional, Hanada, M, additional, Hara, M, additional, Haraguchi, K, additional, Hiratsuka, H, additional, Hirayama, T, additional, Hiroki, S, additional, Hiruta, K, additional, Honda, M, additional, Horiike, H, additional, Hosada, R, additional, Hosogane, N, additional, Iida, K, additional, Iida, Y, additional, Iijima, T, additional, Ikeda, K, additional, Ikeda, Y, additional, Imai, T, additional, Inoue, T, additional, Isaji, N, additional, Isaka, M, additional, Ishida, S, additional, Itami, K, additional, Itige, N, additional, Ito, T, additional, Kakizaki, T, additional, Kamada, Y, additional, Kaminaga, A, additional, Kaneko, T, additional, Kato, T, additional, Kawai, M, additional, Kawabe, M, additional, Kawamata, Y, additional, Kawano, Y, additional, Kawasaki, K, additional, Kikuchi, K, additional, Kikuchi, M, additional, Kimura, H, additional, Kimura, T, additional, Kishimoto, H, additional, Kitamura, S, additional, Kiyono, K, additional, Kobayashi, N, additional, Kodama, K, additional, Kurihata, Y, additional, Koide, Y, additional, Koike, T, additional, Komata, M, additional, Kondo, I, additional, Konoshima, S, additional, Kubo, H, additional, Kunieda, S, additional, Kurihara, K, additional, Kuriyama, M, additional, Kusaka, M, additional, Kusama, Y, additional, Kushima, T, additional, Mabuti, Y, additional, Maehara, S, additional, Maeno, K, additional, Matoba, T, additional, Matsuda, S, additional, Matsukawa, M, additional, Matsukawa, T, additional, Matsuoka, M, additional, Matsuzaki, Y, additional, Miura, Y, additional, Miya, N, additional, Miyachi, K, additional, Miyo, Y, additional, Mizuno, M, additional, Mogaki, K, additional, Moriyama, S, additional, Murakami, Y, additional, Muto, M, additional, Nagami, M, additional, Nagase, K, additional, Nagashima, A, additional, Nagashima, K, additional, Nagashima, T, additional, Nagaya, S, additional, Naito, O, additional, Nakamura, H, additional, Nemoto, H, additional, Nemoto, M, additional, Neyatani, Y, additional, Ninomiya, H, additional, Nishino, N, additional, Nishitani, T, additional, Nobusaka, H, additional, Nomata, H, additional, Obara, K, additional, Odajima, K, additional, Ogawa, Y, additional, Ogiwara, N, additional, Ohga, T, additional, Ohara, Y, additional, Oohara, H, additional, Ohshima, T, additional, Ohta, K, additional, Ohta, M, additional, Ohuchi, S, additional, Ohuchi, Y, additional, Okumura, H, additional, Okumura, Y, additional, Omori, K, additional, Omori, S, additional, Omori, Y, additional, Ozeki, T, additional, Saegusa, M, additional, Saitoh, N, additional, Sakasai, A, additional, Sakata, S, additional, Sasajima, T, additional, Sato, K, additional, Sato, M, additional, Sawahata, M, additional, Sebata, T, additional, Seimiya, M, additional, Seki, M, additional, Seki, S, additional, Shibanuma, K, additional, Shimada, M, additional, Shimada, R, additional, Shimizu, K, additional, Shimizu, M, additional, Shimomura, Y, additional, Shinozaki, S, additional, Shirai, H, additional, Shirakata, H, additional, Shitomi, M, additional, Suganuma, K, additional, Sugawara, T, additional, Sugie, T, additional, Sunaoshi, H, additional, Suzuki, M, additional, Suzuki, N, additional, Suzuki, S, additional, Tachibana, H, additional, Takahashi, M, additional, Takahashi, S, additional, Takahashi, T, additional, Takasaki, M, additional, Takatsu, H, additional, Takeuchi, H, additional, Takeshita, A, additional, Takizuka, T, additional, Tamura, S, additional, Tanaka, S, additional, Tanaka, T, additional, Tanaka, Y, additional, Tani, K, additional, Terakado, M, additional, Terakado, T, additional, Tobita, K, additional, Totsuka, T, additional, Toyoshima, N, additional, Tsuda, F, additional, Tsugita, T, additional, Tsuji, S, additional, Tsukahara, Y, additional, Tsuneoka, M, additional, Uehara, K, additional, Uramoto, Y, additional, Usami, H, additional, Ushigusa, K, additional, Usui, K, additional, Yagyu, J, additional, Yamagiwa, M, additional, Yamamoto, M, additional, Yamashita, O, additional, Yamazaki, T, additional, Yokokura, K, additional, Yokoyama, K, additional, Yoshikawa, K, additional, Yoshida, H, additional, Yoshino, R, additional, Yoshioka, Y, additional, Yonekawa, I, additional, Yoneda, T, additional, and Watanabe, K, additional

Published
1989
Full Text
View/download PDF

46. Characteristics of the JT-60 divertor and limiter plasmas with high power auxiliary heating

Author

Aikawa, H, primary, Akaoka, N, additional, Akasaka, H, additional, Akino, N, additional, Akiyama, T, additional, Ando, T, additional, Annoh, K, additional, Aoyagi, T, additional, Arai, T, additional, Arakawa, K, additional, Araki, M, additional, Azumi, M, additional, Chiba, S, additional, Dairaku, M, additional, Ebisawa, N, additional, Fujii, T, additional, Fukuda, T, additional, Funahashi, A, additional, Furukawa, H, additional, Hamamatsu, K, additional, Hanada, M, additional, Hara, M, additional, Haraguchi, K, additional, Hiratsuka, H, additional, Hirayama, T, additional, Hiroki, S, additional, Hiruta, K, additional, Honda, M, additional, Horiike, H, additional, Hosoda, R, additional, Hosogane, N, additional, Iijima, T, additional, Ikeda, K, additional, Ikeda, Y, additional, Imai, T, additional, Inoue, T, additional, Isaji, N, additional, Isaka, M, additional, Ishida, S, additional, Itami, K, additional, Ichige, N, additional, Itoh, T, additional, Kakizaki, T, additional, Kaminaga, A, additional, Katoh, T, additional, Kawai, M, additional, Kawabe, M, additional, Kawamata, Y, additional, Kawasaki, K, additional, Kikuchi, K, additional, Kikuchi, M, additional, Kimura, H, additional, Kimura, T, additional, Kishimoto, H, additional, Kitamura, S, additional, Kitsunezaki, A, additional, Kiyono, K, additional, Kobayashi, N, additional, Kodama, K, additional, Koide, S, additional, Koide, Y, additional, Koike, T, additional, Komata, M, additional, Kondo, I, additional, Konoshima, S, additional, Kubo, H, additional, Kunieda, S, additional, Kurihara, K, additional, Kuriyama, M, additional, Kuroda, T, additional, Kusaka, M, additional, Kusama, Y, additional, Mabuchi, Y, additional, Maehara, S, additional, Maeno, K, additional, Matoba, T, additional, Matsuda, S, additional, Matsukawa, M, additional, Matsukawa, T, additional, Matsuoka, M, additional, Miura, Y, additional, Miya, N, additional, Miyachi, K, additional, Miyo, Y, additional, Mizuno, M, additional, Mori, M, additional, Moriyama, S, additional, Mutoh, M, additional, Nagami, M, additional, Nagashima, A, additional, Nagashima, K, additional, Nagashima, T, additional, Nagaya, S, additional, Naito, O, additional, Nakamura, H, additional, Nakamura, Y, additional, Nemoto, M, additional, Neyatani, Y, additional, Ninomiya, H, additional, Nishino, N, additional, Nishitani, T, additional, Obara, K, additional, Obinata, H, additional, Ogawa, Y, additional, Ogiwara, N, additional, Ohga, T, additional, Ohara, Y, additional, Ohasa, K, additional, Ohara, H, additional, Ohshima, T, additional, Ohkubo, M, additional, Ohsawa, S, additional, Ohta, K, additional, Ohta, M, additional, Ohtaka, M, additional, Ohuchi, Y, additional, Oikawa, A, additional, Okumura, H, additional, Okumura, Y, additional, Omori, K, additional, Omori, S, additional, Omori, Y, additional, Ozeki, T, additional, Saegusa, M, additional, Saitoh, N, additional, Sakamoto, K, additional, Sakasai, A, additional, Sakata, S, additional, Sasajima, T, additional, Satou, K, additional, Satou, M, additional, Sakurai, A, additional, Sawahata, M, additional, Sebata, T, additional, Seimiya, M, additional, Seki, M, additional, Seki, S, additional, Shibanuma, K, additional, Shimada, R, additional, Shimada, T, additional, Shimizu, K, additional, Shimizu, M, additional, Shimomura, Y, additional, Shinozaki, S, additional, Shirai, H, additional, Shirakata, H, additional, Shitomi, M, additional, Suganuma, K, additional, Sugie, T, additional, Sugiyama, T, additional, Sunaoshi, H, additional, Suzuki, K, additional, Suzuki, M, additional, Suzuki, N, additional, Suzuki, S, additional, Suzuki, Y, additional, Takahashi, M, additional, Takahashi, S, additional, Takahashi, T, additional, Takasaki, M, additional, Takatsu, H, additional, Takeuchi, H, additional, Takeshita, A, additional, Takizuka, T, additional, Tamura, S, additional, Tanaka, S, additional, Tani, K, additional, Terakado, M, additional, Terakado, T, additional, Tobita, K, additional, Tokutake, T, additional, Totsuka, T, additional, Toyoshima, N, additional, Tsuda, F, additional, Tsugita, T, additional, Tsuji, S, additional, Tsukahara, Y, additional, Tsuneoka, M, additional, Uehara, K, additional, Umehara, M, additional, Uramoto, Y, additional, Usami, H, additional, Ushigusa, K, additional, Usui, K, additional, Yagyu, J, additional, Yamagiwa, M, additional, Yamamoto, M, additional, Yamamoto, T, additional, Yamashita, O, additional, Yamazaki, T, additional, Yasukawa, T, additional, Yokokura, K, additional, Yokomizo, H, additional, Yokoyama, K, additional, Yoshikawa, K, additional, Yoshikawa, M, additional, Yoshida, H, additional, Yoshino, R, additional, Yoshioka, Y, additional, Yonekawa, I, additional, Yoneda, T, additional, Watanabe, K, additional, Bell, M.G, additional, Bickerton, R.J, additional, Engelhardt, W, additional, Goldston, R.J, additional, Ilne, E.K, additional, Kaline, J, additional, Kugel, H.W, additional, Mondino, P.L, additional, Soldner, F.X, additional, Takase, Y, additional, Thomas, P.R, additional, and Wong, K.L, additional

Published
1989
Full Text
View/download PDF

48. High power heating results on JT-60

Author

Akiba, M, primary, Aikawa, H, additional, Akaoka, N, additional, Akasaka, H, additional, Akino, N, additional, Akiyama, T, additional, Ando, T, additional, Annoh, K, additional, Aoyagi, T, additional, Arai, T, additional, Arakawa, K, additional, Araki, M, additional, Azumi, M, additional, Chiba, S, additional, Dairaku, M, additional, Ebisawa, N, additional, Fujii, T, additional, Fukuda, T, additional, Funahashi, A, additional, Furukawa, H, additional, Hamamatsu, K, additional, Hanada, M, additional, Hara, M, additional, Haraguchi, K, additional, Hiratsuka, H, additional, Hirayama, T, additional, Hiroki, S, additional, Hiruta, K, additional, Honda, M, additional, Horiike, H, additional, Hosoda, R, additional, Hosogane, N, additional, Iijima, T, additional, Ikeda, Y, additional, Ikeda, K, additional, Imai, T, additional, Inoue, T, additional, Isaji, N, additional, Isaka, M, additional, Ishida, S, additional, Itami, K, additional, Ichige, N, additional, Itoh, T, additional, Kakizaki, T, additional, Kaminaga, A, additional, Katoh, T, additional, Kawai, M, additional, Kawabe, M, additional, Kawamata, Y, additional, Kawasaki, K, additional, Kikuchi, K, additional, Kikuchi, M, additional, Kimura, H, additional, Kimura, T, additional, Kishimoto, H, additional, Kitamura, S, additional, Kitsunezaki, A, additional, Kiyono, K, additional, Kobayashi, N, additional, Kodama, K, additional, Koide, S, additional, Koide, Y, additional, Kioke, T, additional, Komata, M, additional, Kondo, I, additional, Konoshima, S, additional, Kubo, H, additional, Kunieda, S, additional, Kurihara, K, additional, Kuriyama, M, additional, Kuroda, T, additional, Kusaka, M, additional, Kusama, Y, additional, Mabuchi, Y, additional, Maehara, S, additional, Maeno, K, additional, Matoba, T, additional, Matsuda, S, additional, Matsukawa, M, additional, Matsukawa, T, additional, Matsuoka, M, additional, Miura, Y, additional, Miya, N, additional, Miyachi, K, additional, Mori, Y, additional, Moriyama, S, additional, Mutoh, M, additional, Nagami, M, additional, Nagashima, A, additional, Nagashima, K, additional, Nagashima, T, additional, Nagaya, S, additional, Naitoh, O, additional, Nakamura, H, additional, Nakamura, Y, additional, Nemoto, M, additional, Neyatani, Y, additional, Ninomiya, H, additional, Nishino, N, additional, Nishitani, T, additional, Obara, K, additional, Obinata, H, additional, Ogawa, Y, additional, Ogiwara, N, additional, Ohga, T, additional, Ohara, Y, additional, Ohasa, K, additional, Oohara, H, additional, Ohshima, T, additional, Ohkubo, M, additional, Ohsawa, S, additional, Ohta, K, additional, Ohta, M, additional, Ohtaka, M, additional, Ohuchi, Y, additional, Oikawa, A, additional, Okumura, H, additional, Okumura, Y, additional, Omori, K, additional, Omori, S, additional, Omori, Y, additional, Ozeki, T, additional, Saegusa, M, additional, Saitoh, N, additional, Sakamoto, K, additional, Sakasai, A, additional, Sakata, S, additional, Sasajima, T, additional, Satou, K, additional, Satou, M, additional, Sakurai, A, additional, Sawahata, M, additional, Sebata, T, additional, Seimiya, M, additional, Seki, M, additional, Seki, S, additional, Shibanuma, K, additional, Shimada, R, additional, Shimada, T, additional, Shimizu, K, additional, Shimizu, M, additional, Shimomura, Y, additional, Shinozaki, S, additional, Shirai, H, additional, Shirakata, H, additional, Shitomi, M, additional, Suganuma, K, additional, Sugie, T, additional, Sugiyama, T, additional, Sunaoshi, H, additional, Suzuki, K, additional, Suzuki, M, additional, Suzuki, N, additional, Suzuki, S, additional, Suzuki, Y, additional, Takahashi, M, additional, Takahashi, S, additional, Takahashi, T, additional, Takasaki, M, additional, Takatsu, H, additional, Takeuchi, H, additional, Takeshita, A, additional, Takizuka, T, additional, Tamura, S, additional, Tanaka, S, additional, Tanaka, T, additional, Tani, K, additional, Terakado, M, additional, Terakado, T, additional, Tobita, K, additional, Tokutake, T, additional, Totsuka, T, additional, Toyoshima, N, additional, Tusda, F, additional, Tsugita, T, additional, Tsuji, S, additional, Tsukahara, Y, additional, Tsuneoka, M, additional, Uehara, K, additional, Umechara, M, additional, Uramoto, Y, additional, Usami, H, additional, Ushigusa, K, additional, Usui, K, additional, Yagyu, J, additional, Yamagiwa, M, additional, Yamamoto, M, additional, Yamamoto, T, additional, Yamashita, O, additional, Yamazaki, T, additional, Yasukawa, T, additional, Yokokura, K, additional, Yokomizo, H, additional, Yokoyama, K, additional, Yoshikawa, K, additional, Yoshikawa, M, additional, Yoshida, H, additional, Yoshino, R, additional, Yoshioka, Y, additional, Yonekawa, I, additional, Yoneda, T, additional, Watanabe, K, additional, Bell, M G, additional, Bickerton, R J, additional, Englehardt, W, additional, Goldston, R J, additional, Kallne, E, additional, Kallne, J, additional, Jugel, H W, additional, Mondiono, P L, additional, Solnder, F X, additional, Takase, Y, additional, Thomas, P R, additional, and Wong, K L, additional

Published
1988
Full Text
View/download PDF

49. Characteristics of the JT-60 divertor and limiter plasmas with high power auxiliary heating

Author

The JT-60 Team, Aikawa, H, Akaoka, N, Akasaka, H, Akino, N, Akiyama, T, Ando, T, Annoh, K, Aoyagi, T, Arai, T, Arakawa, K, Araki, M, Azumi, M, Chiba, S, Dairaku, M, Ebisawa, N, Fujii, T, Fukuda, T, Funahashi, A, Furukawa, H, Hamamatsu, K, Hanada, M, Hara, M, Haraguchi, K, Hiratsuka, H, Hirayama, T, Hiroki, S, Hiruta, K, Honda, M, Horiike, H, Hosoda, R, Hosogane, N, Iijima, T, Ikeda, K, Ikeda, Y, Imai, T, Inoue, T, Isaji, N, Isaka, M, Ishida, S, Itami, K, Ichige, N, Itoh, T, Kakizaki, T, Kaminaga, A, Katoh, T, Kawai, M, Kawabe, M, Kawamata, Y, Kawasaki, K, Kikuchi, K, Kikuchi, M, Kimura, H, Kimura, T, Kishimoto, H, Kitamura, S, Kitsunezaki, A, Kiyono, K, Kobayashi, N, Kodama, K, Koide, S, Koide, Y, Koike, T, Komata, M, Kondo, I, Konoshima, S, Kubo, H, Kunieda, S, Kurihara, K, Kuriyama, M, Kuroda, T, Kusaka, M, Kusama, Y, Mabuchi, Y, Maehara, S, Maeno, K, Matoba, T, Matsuda, S, Matsukawa, M, Matsukawa, T, Matsuoka, M, Miura, Y, Miya, N, Miyachi, K, Miyo, Y, Mizuno, M, Mori, M, Moriyama, S, Mutoh, M, Nagami, M, Nagashima, A, Nagashima, K, Nagashima, T, Nagaya, S, Naito, O, Nakamura, H, Nakamura, Y, Nemoto, M, Neyatani, Y, Ninomiya, H, Nishino, N, Nishitani, T, Obara, K, Obinata, H, Ogawa, Y, Ogiwara, N, Ohga, T, Ohara, Y, Ohasa, K, Ohara, H, Ohshima, T, Ohkubo, M, Ohsawa, S, Ohta, K, Ohta, M, Ohtaka, M, Ohuchi, Y, Oikawa, A, Okumura, H, Okumura, Y, Omori, K, Omori, S, Omori, Y, Ozeki, T, Saegusa, M, Saitoh, N, Sakamoto, K, Sakasai, A, Sakata, S, Sasajima, T, Satou, K, Satou, M, Sakurai, A, Sawahata, M, Sebata, T, Seimiya, M, Seki, M, Seki, S, Shibanuma, K, Shimada, R, Shimada, T, Shimizu, K, Shimizu, M, Shimomura, Y, Shinozaki, S, Shirai, H, Shirakata, H, Shitomi, M, Suganuma, K, Sugie, T, Sugiyama, T, Sunaoshi, H, Suzuki, K, Suzuki, M, Suzuki, N, Suzuki, S, Suzuki, Y, Takahashi, M, Takahashi, S, Takahashi, T, Takasaki, M, Takatsu, H, Takeuchi, H, Takeshita, A, Takizuka, T, Tamura, S, Tanaka, S, Tani, K, Terakado, M, Terakado, T, Tobita, K, Tokutake, T, Totsuka, T, Toyoshima, N, Tsuda, F, Tsugita, T, Tsuji, S, Tsukahara, Y, Tsuneoka, M, Uehara, K, Umehara, M, Uramoto, Y, Usami, H, Ushigusa, K, Usui, K, Yagyu, J, Yamagiwa, M, Yamamoto, M, Yamamoto, T, Yamashita, O, Yamazaki, T, Yasukawa, T, Yokokura, K, Yokomizo, H, Yokoyama, K, Yoshikawa, K, Yoshikawa, M, Yoshida, H, Yoshino, R, Yoshioka, Y, Yonekawa, I, Yoneda, T, Watanabe, K, Bell, M.G, Bickerton, R.J, Engelhardt, W, Goldston, R.J, Ilne, E.K, Kaline, J, Kugel, H.W, Mondino, P.L, Soldner, F.X, Takase, Y, Thomas, P.R, and Wong, K.L

Published
1989
Full Text
View/download PDF

50. S100A4 contributes to colorectal carcinoma aggressive behavior and to chemoradiotherapy resistance in locally advanced rectal carcinoma.

Author

Harada Y, Ikeda S, Kawabe Y, Oguri Y, Hashimura M, Yokoi A, Sida A, Fukagawa N, Hayashi M, Ono M, Kusano C, Takahashi H, and Saegusa M

Subjects
Humans, Male, Female, Middle Aged, HCT116 Cells, Aged, beta Catenin metabolism, Tumor Suppressor Protein p53 metabolism, Tumor Suppressor Protein p53 genetics, Prognosis, Cell Proliferation, Apoptosis, Adult, Neoadjuvant Therapy, Gene Expression Regulation, Neoplastic, S100 Calcium-Binding Protein A4 metabolism, S100 Calcium-Binding Protein A4 genetics, Rectal Neoplasms metabolism, Rectal Neoplasms pathology, Rectal Neoplasms therapy, Rectal Neoplasms genetics, Colorectal Neoplasms metabolism, Colorectal Neoplasms pathology, Colorectal Neoplasms genetics, Colorectal Neoplasms therapy, Chemoradiotherapy, Drug Resistance, Neoplasm
Abstract

To investigate the functional role of S100A4 in advanced colorectal carcinoma (Ad-CRC) and locally advanced rectal carcinoma (LAd-RC) receiving neoadjuvant chemoradiotherapy (NCRT). We analyzed histopathological and immunohistochemical sections from 150 patients with Ad-CRC and 177 LAd-RC patients treated with NCRT. S100A4 knockout (KO) HCT116 cells were also used. S100A4 expression was absent in normal mucosa but increased progressively from colorectal adenoma to carcinoma, suggesting that S100A4 regulation is an early event in colorectal carcinogenesis. In Ad-CRC, high S100A4 expression correlated with high tumor budding and nuclear β-catenin, deep invasion, lymph-vascular involvement, and unfavorable prognosis. In NCRT-treated LAd-RC, high S100A4 expression was associated with poor treatment response and short progression-free survival. S100A4 KO decreased the proliferation of HCT116 cells through activation of the p53/p21 waf1 axis, and sensitized cells to adriamycin-induced apoptosis. Levels of the apoptotic marker, cleaved poly (ADP-ribose) polymerase 1, were significantly higher in samples with low S100A4 and wild type p53. Finally, we observed a direct interaction between S100A4 and p53. In conclusion, S100A4 expression engenders aggressive behavior in Ad-CRC through association with β-catenin-driven tumor buddings. S100A4 exerts anti-apoptotic and proliferative effects via inhibition of p53 in LAd-RC patients receiving NCRT, which leads to chemoradioresistance and poor prognosis., Competing Interests: Declarations. Competing interests: The authors declare no competing interests. Consent for publication: Not applicable. Ethics approval: This study was conducted in accordance with the principles expressed in the Declaration of Helsinki 1964 and later versions. This study was approved by the Kitasato University Medical Ethics Committee (B19-155). This article does not contain any studies with animals performed by any of the authors. Informed consent: was obtained from all subjects involved in the study., (© 2024. The Author(s).)

Published
2024
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results