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1. Detection of Mycobacterium avium subsp. paratuberculosis in infected tissues by new species-specific immunohistological procedures.

Author

UCL - MD/MNOP - Département de morphologie normale et pathologique, UCL - MD/MINT - Département de médecine interne, UCL - MD/CHIR - Département de chirurgie, Coetsier, C, Denef, Jean-François, Havaux, Xavier, Mattelard, F, Sadatte, S, Cormont, F., Buergelt, K, Limbourg, B, Latinne, Dominique, Bazin, Hervé, Cocito, Carlo, UCL - MD/MNOP - Département de morphologie normale et pathologique, UCL - MD/MINT - Département de médecine interne, UCL - MD/CHIR - Département de chirurgie, Coetsier, C, Denef, Jean-François, Havaux, Xavier, Mattelard, F, Sadatte, S, Cormont, F., Buergelt, K, Limbourg, B, Latinne, Dominique, Bazin, Hervé, and Cocito, Carlo

Abstract

We have previously described the cloning and sequencing of a gene portion coding for the terminal part of a 34-kDa protein of Mycobacterium avium subsp. paratuberculosis, the etiological agent of Johne's disease (P. Gilot, M. De Kesel, L. Machtelinckx, M. Coene, and C. Cocito, J. Bacteriol. 175:4930-4935, 1993). The recombinant polypeptide (a362) carries species-specific B-cell epitopes which do not cross-react with other mycobacterial pathogens (M. De Kesel, P. Gilot, M.-C. Misonne, M. Coene, and C. Cocito, J. Clin. Microbiol. 31:947-954, 1993). The present work describes the preparation of polyclonal and monoclonal antibodies directed against a362 and the use of these immunoglobulins for histopathological diagnosis of Johne's disease. The new immunohistological procedures herewith detailed proved to be able to identify M. avium subsp. paratuberculosis antigens in the intestinal tissues and lymph nodes of cattle affected by either the paucibacillary or pluribacillary form of the disease. They yielded negative responses not only with healthy animals but also with those affected by tuberculosis (Mycobacterium bovis). Both immunohistological procedures proved to be as sensitive as or more sensitive than Ziehl-Neelsen staining and, in addition, to be endowed with species specificity.

Published
1998

2. Detection of Mycobacterium avium subsp. paratuberculosis in infected tissues by new species-specific immunohistological procedures.

Author

Coetsier, C, Havaux, X, Mattelard, F, Sadatte, S, Cormont, F, Buergelt, K, Limbourg, B, Latinne, D, Bazin, H, Denef, J F, and Cocito, C

Abstract

We have previously described the cloning and sequencing of a gene portion coding for the terminal part of a 34-kDa protein of Mycobacterium avium subsp. paratuberculosis, the etiological agent of Johne's disease (P. Gilot, M. De Kesel, L. Machtelinckx, M. Coene, and C. Cocito, J. Bacteriol. 175:4930-4935, 1993). The recombinant polypeptide (a362) carries species-specific B-cell epitopes which do not cross-react with other mycobacterial pathogens (M. De Kesel, P. Gilot, M.-C. Misonne, M. Coene, and C. Cocito, J. Clin. Microbiol. 31:947-954, 1993). The present work describes the preparation of polyclonal and monoclonal antibodies directed against a362 and the use of these immunoglobulins for histopathological diagnosis of Johne's disease. The new immunohistological procedures herewith detailed proved to be able to identify M. avium subsp. paratuberculosis antigens in the intestinal tissues and lymph nodes of cattle affected by either the paucibacillary or pluribacillary form of the disease. They yielded negative responses not only with healthy animals but also with those affected by tuberculosis (Mycobacterium bovis). Both immunohistological procedures proved to be as sensitive as or more sensitive than Ziehl-Neelsen staining and, in addition, to be endowed with species specificity.

Published
1998

3. Detection of Mycobacterium avium subsp. paratuberculosis in infected tissues by new species-specific immunohistological procedures.

Author

Coetsier C, Havaux X, Mattelard F, Sadatte S, Cormont F, Buergelt K, Limbourg B, Latinne D, Bazin H, Denef JF, and Cocito C

Subjects
Animals, Antibodies, Bacterial, Antibodies, Monoclonal, Antibody Specificity, Antigens, Bacterial genetics, Bacterial Proteins genetics, Bacterial Proteins immunology, Cattle, Cattle Diseases diagnosis, Cattle Diseases immunology, Cattle Diseases microbiology, Cattle Diseases pathology, Genes, Bacterial, Histological Techniques, Mycobacterium avium subsp. paratuberculosis genetics, Paratuberculosis diagnosis, Paratuberculosis immunology, Paratuberculosis microbiology, Paratuberculosis pathology, Rabbits, Rats, Recombinant Proteins genetics, Recombinant Proteins immunology, Sensitivity and Specificity, Species Specificity, Immunologic Techniques statistics & numerical data, Mycobacterium avium subsp. paratuberculosis immunology, Mycobacterium avium subsp. paratuberculosis isolation & purification
Abstract

We have previously described the cloning and sequencing of a gene portion coding for the terminal part of a 34-kDa protein of Mycobacterium avium subsp. paratuberculosis, the etiological agent of Johne's disease (P. Gilot, M. De Kesel, L. Machtelinckx, M. Coene, and C. Cocito, J. Bacteriol. 175:4930-4935, 1993). The recombinant polypeptide (a362) carries species-specific B-cell epitopes which do not cross-react with other mycobacterial pathogens (M. De Kesel, P. Gilot, M.-C. Misonne, M. Coene, and C. Cocito, J. Clin. Microbiol. 31:947-954, 1993). The present work describes the preparation of polyclonal and monoclonal antibodies directed against a362 and the use of these immunoglobulins for histopathological diagnosis of Johne's disease. The new immunohistological procedures herewith detailed proved to be able to identify M. avium subsp. paratuberculosis antigens in the intestinal tissues and lymph nodes of cattle affected by either the paucibacillary or pluribacillary form of the disease. They yielded negative responses not only with healthy animals but also with those affected by tuberculosis (Mycobacterium bovis). Both immunohistological procedures proved to be as sensitive as or more sensitive than Ziehl-Neelsen staining and, in addition, to be endowed with species specificity.

Published
1998
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