Search

Your search keyword '"Sachiho Nagashio"' showing total 16 results
Start Over Author "Sachiho Nagashio"
16 results on '"Sachiho Nagashio"'

1. Presence of periodontitis may synergistically contribute to cancer progression via Treg and IL-6

Author

Ryo Kajihara, Hironori Sakai, Yibing Han, Kei Amari, Makiko Kawamoto, Yusuke Hakoyama, Sachiho Nagashio, Shin-ichi Yamada, Hideki Sanjo, and Hiroshi Kurita

Subjects
Medicine, Science
Abstract

Abstract A close causal relationship has been suggested to exist between cancer and periodontitis. We hypothesized that the immune surveillance system is impaired in patients with periodontitis, which contributes to cancer development and growth. Therefore, the present study investigated the relationship between immune surveillance mechanisms and periodontitis in cancer patients. The presence or absence of periodontitis was assessed and the peripheral blood (PB) concentrations of IL-6, immunosuppressive cytokines (VEGF, TGF-β1, and CCL22) and proportion of T regulatory cells (Treg, CD3 + CD4 + CD25 + Foxp3 +) were measured. Subjects were classified into the following four groups: non-cancer patients without periodontitis (C − P −), non-cancer patients with periodontitis (C − P +), cancer patients without periodontitis (C + P −), and cancer patients with periodontitis (C + P +). The results of a multivariate analysis showed that the PB concentration of IL-6 was significantly higher in C + than in C- and higher in C + P + than in C + P −. The PB proportion of Treg was significantly higher in C + P + than in C + P −, C − P + , and C − P −. The results of this study suggested that the presence of periodontitis and cancer synergistically increased Treg in PB, which may be one of the underlying causes of immunosuppression and immune evasion in cancer. It was also suggested that the presence of periodontal disease and/or cancer also increases IL-6 in PB, which would be associated with cancer progression. These results suggest the possibility that the presence of periodontitis might synergistically contribute to cancer progression.

Published
2022
Full Text
View/download PDF

2. Water intake accelerates ATP release from myofibroblast cells in rats: ATP-mediated podoplanin-dependent control for physiological function and immunity

Author

Tomomi Watanabe-Asaka, Nariaki Arai, Ryo Kajihara, Toshio Ohhashi, Yumiko Yokoyama, Daisuke Maejima, Moyuru Hayashi, Maki Kaidoh, Yoshiko Kawai, Kei Amari, and Sachiho Nagashio

Subjects
0301 basic medicine, Physiology, Drinking, Stimulation, Interleukin 22, 03 medical and health sciences, Adenosine Triphosphate, 0302 clinical medicine, Downregulation and upregulation, Physiology (medical), Intestine, Small, medicine, Humans, Lymphocytes, Intestinal Mucosa, Myofibroblasts, Immunity, Mucosal, Lamina propria, Membrane Glycoproteins, Hepatology, ATP synthase, biology, Chemistry, Innate lymphoid cell, Gastroenterology, Immunity, Innate, Cell biology, 030104 developmental biology, medicine.anatomical_structure, Podoplanin, biology.protein, Myofibroblast, 030217 neurology & neurosurgery, Transcription Factors
Abstract

We previously demonstrated that water intake increased mesenteric lymph flow and the total flux of IL-22 in rat jejunum. The drained water and the higher permeability of albumin in the jejunal microcirculation contributed to increase the lymph flow and IL-22 transport via the activation of great bulk flow in the jejunal villi. To address the effects of water intake-mediated great bulk flow-dependent mechanical force on jejunal physiological function and immunological regulation of innate lymphoid cells (ILC)-3, we examined the effects of shear stress stimulation on cultured rat myofibroblast cells. Next, we investigated the effects of water intake on podoplanin and IL-22 expressions in cultured human intestinal epithelial cells and rat in vivo jejunal preparations, respectively. Shear stress stimulation of the myofibroblast cells induced ATP release via an activation of cell surface F1/F0 ATP synthase. ATP produced podoplanin expression in the intestinal epithelial cells. Water intake accelerated immunohistochemical expressions of podoplanin and IL-22 in the interepithelial layers and lamina propria of the jejunum. ATP dose-dependently increased IL-22 mRNA expression in ILC-3, which are housed in the lamina propria. Water intake also increased immunohistochemical and mRNA expressions of ecto-nucleoside triphosphate diphosphohydrolases 2 and 5 in jejunal villi. In conclusion, water intake-mediated shear stress stimulation-dependent ATP release from myofibroblast cells maintains higher tissue colloid osmotic pressure in the jejunal microcirculation through podoplanin upregulation in the interepithelial layers. ATP induces IL-22 mRNA expression in ILC-3 in jejunal villi, which may contribute to regulation of mucosal immunity in small intestine.NEW & NOTEWORTHY We investigated effects of shear stress stimulation on cultured myofibroblast cells and water intake on podoplanin and IL-22 expressions in rat jejunal villi. The stimulation induced ATP release from the cells. Water intake accelerated podoplanin and IL-22 expression levels. ATP increased IL-22 mRNA expression in innate lymphoid cells (ILC)-3. Hence, water intake maintains higher osmotic pressure in the jejunal villi through ATP release and podoplanin upregulation. Water intake may regulate the mucosal immunity.

Published
2021
Full Text
View/download PDF

5. Water intake releases serotonin from enterochromaffin cells in rat jejunal villi

Author

Tomomi Watanabe-Asaka, Toshio Ohhashi, Nariaki Arai, Kei Amari, Yoshiko Kawai, Maki Kaidoh, Moyuru Hayashi, Daisuke Maejima, Sachiho Nagashio, Ryo Kajihara, and Yumiko Yokoyama

Subjects
0301 basic medicine, medicine.medical_specialty, Ketanserin, Physiology, Chemistry, Clinical Biochemistry, Albumin, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Endocrinology, Distilled water, Physiology (medical), Internal medicine, cardiovascular system, medicine, Enterochromaffin cell, Serotonin Production, Serotonin, Lymph, Receptor, 030217 neurology & neurosurgery, medicine.drug
Abstract

The present study aims to investigate the roles of water intake in serotonin production and release in rat jejunum. We evaluated the changes in concentrations of serotonin in the portal vein and mesenteric lymph vessel induced by the intragastric administration of distilled water. The density of granules in enterochromaffin cells and the immunoreactivity of serotonin in the jejunal villi were investigated before and after water intake. The effects of intravenous administration of serotonin and/or ketanserin on mesenteric lymph flow and concentrations of albumin and IL-22 in the lymph were also addressed. Water intake increased serotonin concentration in the portal vein, but not in the mesenteric lymph vessel. The flux of serotonin through the portal vein was significantly larger than that through the mesenteric lymph vessel. Water intake decreased the density of granules in the enterochromaffin cells and increased the immunoreactivity of serotonin in the jejunal villi. The intravenous administration of serotonin increased significantly mesenteric lymph flow and the concentrations of albumin and IL-22; both were significantly reduced by the intravenous pretreatment with ketanserin. We showed that serotonin released from enterochromaffin cells by water intake was mainly transported through the portal vein. Additionally, serotonin in blood was found to increase mesenteric lymph formation with permeant albumin in the jejunal villi via the activation of 5-HT2 receptor.

Published
2021
Full Text
View/download PDF

6. Water intake releases serotonin from enterochromaffin cells in rat jejunal villi

Author

Ryo, Kajihara, Kei, Amari, Nariaki, Arai, Sachiho, Nagashio, Moyuru, Hayashi, Tomomi, Watanabe-Asaka, Maki, Kaidoh, Yumiko, Yokoyama, Daisuke, Maejima, Yoshiko, Kawai, and Toshio, Ohhashi

Subjects
Male, Rats, Sprague-Dawley, Serotonin, Jejunum, Portal Vein, Albumins, Interleukins, Drinking, Enterochromaffin Cells, Animals, Cytoplasmic Granules, Rats
Abstract

The present study aims to investigate the roles of water intake in serotonin production and release in rat jejunum. We evaluated the changes in concentrations of serotonin in the portal vein and mesenteric lymph vessel induced by the intragastric administration of distilled water. The density of granules in enterochromaffin cells and the immunoreactivity of serotonin in the jejunal villi were investigated before and after water intake. The effects of intravenous administration of serotonin and/or ketanserin on mesenteric lymph flow and concentrations of albumin and IL-22 in the lymph were also addressed. Water intake increased serotonin concentration in the portal vein, but not in the mesenteric lymph vessel. The flux of serotonin through the portal vein was significantly larger than that through the mesenteric lymph vessel. Water intake decreased the density of granules in the enterochromaffin cells and increased the immunoreactivity of serotonin in the jejunal villi. The intravenous administration of serotonin increased significantly mesenteric lymph flow and the concentrations of albumin and IL-22; both were significantly reduced by the intravenous pretreatment with ketanserin. We showed that serotonin released from enterochromaffin cells by water intake was mainly transported through the portal vein. Additionally, serotonin in blood was found to increase mesenteric lymph formation with permeant albumin in the jejunal villi via the activation of 5-HT

Published
2021

7. Relationship between dry mouth and hypertension

Author

Hirokazu Tanaka, Atsushi Takizawa, Takahiko Gibo, Ryo Kajihara, Hironori Sakai, Shin-ichi Yamada, Hiroshi Kurita, Junichi Yajima, Tomoki Kaneko, Sachiho Nagashio, Eiji Kondo, Takeshi Uehara, and Makiko Kawamoto

Subjects
medicine.medical_specialty, business.industry, Submandibular Gland, 030206 dentistry, Arteriosclerosis, Degeneration (medical), medicine.disease, Dry mouth, Gastroenterology, Xerostomia, 03 medical and health sciences, Stenosis, 0302 clinical medicine, stomatognathic system, 030220 oncology & carcinogenesis, Internal medicine, Epidemiology, Hypertension, Medicine, Humans, In patient, Fatty infiltration, medicine.symptom, business, General Dentistry
Abstract

Salivary dysfunction, such as reduced salivary flow and an altered salivary composition, is caused by several diseases, medical conditions, and medications. The purpose of the present study was to clarify the relationship between hypertension and morphological changes in the submandibular glands. An epidemiological study was conducted to elucidate the relationship between hypertension and dry mouth. The effects of hypertension on morphological changes and the intima thickness of arteries in the submandibular glands were histopathologically investigated. Among 1933 subjects in the epidemiological study, 155 (8.0%) had dry mouth. A multivariate analysis revealed that dry mouth correlated with age (p

Published
2020

8. Water intake increases mesenteric lymph flow and the total flux of albumin, long-chain fatty acids, and IL-22 in rats: new concept of absorption in jejunum

Author

Tomomi Watanabe-Asaka, Ryo Kajihara, Daisuke Maejima, Moyuru Hayashi, Yoshiko Kawai, Yumiko Yokoyama, Hideki Sanjo, Shinshuke Taki, Maki Kaidoh, Kumiko Ajima, Toshio Ohhashi, and Sachiho Nagashio

Subjects
Male, 0301 basic medicine, medicine.medical_specialty, Physiology, Drinking, Intestinal absorption, Jejunum, Interleukin 22, 03 medical and health sciences, 0302 clinical medicine, Albumins, Physiology (medical), Internal medicine, medicine, Animals, Lymphocytes, Water intake, Lymphatic Vessels, Hepatology, Chemistry, Interleukins, Fatty Acids, Gastroenterology, Albumin, Immunity, Innate, Small intestine, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, Intestinal Absorption, Liver, 030211 gastroenterology & hepatology, Lymph, Rabbits, Long chain
Abstract

The traditional Japanese health care custom recommends that a suitable volume of water is consumed. However, physiological and immunological mechanisms in support of this practice are unknown. Therefore, we conducted rat and rabbit in vivo experiments to investigate the effects of intragastric administration of distilled water on the jejunal-originated lymph flow and the concentrations and total flux of cells, albumin, long-chain fatty acids, and innate lymphoid cell 3 (ILC-3)-secreted interleukin-22 (IL-22) through mesenteric lymph vessels. The distribution and activity of ILC-3 in rat small intestine by water intake were evaluated using flow cytometry and RT-PCR. The intragastric administration of distilled water caused significant increases in rat mesenteric lymph flow and in the total flux of cells, albumin, long-chain fatty acids, and IL-22 through the lymph vessels. Intravenously injected Evans blue dye was rapidly transported into rabbit mesenteric lymph vessel and cisterna chyli. The distribution of ILC-3 and the expression of IL-22 mRNA were maximal in the lamina propria cells of the rat jejunum. No significant presence of ILC-3 in the lymph was observed in the control and under water intake conditions. In conclusion, the absorbed water in the jejunum is transported through mesenteric lymph vessels. The higher permeability of albumin in the jejunal microcirculation may play key roles in the transport of consumed water and the reservoir and transporter of long-chain fatty acids. Water intake also accelerates the transfer of IL-22 to the mesenteric lymph, which may contribute, in part, to maintaining and promoting the innate immunity in the body. NEW & NOTEWORTHY The higher permeability of albumin-mediated transport of water-soluble substances in mesenteric lymph vessels of the jejunum may have a large impact on the classic concept suggesting that water-soluble small molecules travel to the liver via the portal vein. ILC-3 is mainly housed in the lamina propria of the jejunum, especially its upper part. IL-22 released from the ILC-3 is also transported through mesenteric lymph in collaboration with the albumin-mediated movement of consumed water.

Published
2019
Full Text
View/download PDF

10. Evaluating Lymph Flow Through the Thoracic Duct Using Urine Osmolarity in Human Participants

Author

Sachiho Nagashio, Yoshiko Kawai, Toshio Ohhashi, Takayuki Honda, Tomomi Watanabe-Asaka, Kei Amari, Moyuru Hayashi, Mitsutoshi Sugano, Ryo Kajihara, Yumiko Yokoyama, Maki Kaidoh, and Daisuke Maejima

Subjects
medicine.medical_specialty, Urology, Diaphragmatic breathing, 030204 cardiovascular system & hematology, Thoracic duct, Thoracic Duct, 03 medical and health sciences, 0302 clinical medicine, Chlorides, Intragastric administration, medicine, Humans, Water intake, Human studies, business.industry, fungi, Osmolar Concentration, Sodium, food and beverages, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Lymph flow, Urine osmolality, Animal studies, Lymph, Cardiology and Cardiovascular Medicine, business
Abstract

Background: Previous animal studies have shown that intragastric administration of water can accelerate mesenteric lymph flow. Similarly, human studies have shown that abdominal breathing can induc...

Published
2020

12. Water intake accelerates ATP release from myofibroblast cells in rats: ATPmediated podoplanin-dependent control for physiological function and immunity.

Author

Moyuru Hayashi, Tomomi Watanabe-Asaka, Sachiho Nagashio, Maki Kaidoh, Yumiko Yokoyama, Daisuke Maejima, Ryo Kajihara, Kei Amari, Nariaki Arai, Yoshiko Kawai, and Toshio Ohhashi

Abstract

We previously demonstrated that water intake increased mesenteric lymph flow and the total flux of IL-22 in rat jejunum. The drained water and the higher permeability of albumin in the jejunal microcirculation contributed to increase the lymph flow and IL-22 transport via the activation of great bulk flow in the jejunal villi. To address the effects of water intake-mediated great bulk flow-dependent mechanical force on jejunal physiological function and immunological regulation of innate lymphoid cells (ILC)-3, we examined the effects of shear stress stimulation on cultured rat myofibroblast cells. Next, we investigated the effects of water intake on podoplanin and IL-22 expressions in cultured human intestinal epithelial cells and rat in vivo jejunal preparations, respectively. Shear stress stimulation of the myofibroblast cells induced ATP release via an activation of cell surface F1/F0 ATP synthase. ATP produced podoplanin expression in the intestinal epithelial cells. Water intake accelerated immunohistochemical expressions of podoplanin and IL-22 in the interepithelial layers and lamina propria of the jejunum. ATP dose-dependently increased IL-22 mRNA expression in ILC-3, which are housed in the lamina propria. Water intake also increased immunohistochemical and mRNA expressions of ecto-nucleoside triphosphate diphosphohydrolases 2 and 5 in jejunal villi. In conclusion, water intake-mediated shear stress stimulation-dependent ATP release from myofibroblast cells maintains higher tissue colloid osmotic pressure in the jejunal microcirculation through podoplanin upregulation in the interepithelial layers. ATP induces IL-22 mRNA expression in ILC-3 in jejunal villi, which may contribute to regulation of mucosal immunity in small intestine. [ABSTRACT FROM AUTHOR]

Published
2021
Full Text
View/download PDF

13. Evaluating Lymph Flow Through the Thoracic Duct Using Urine Osmolarity in Human Participants.

Author

Moyuru Hayashi, Tomomi Watanabe-Asaka, Daisuke Maejima, Sachiho Nagashio, Ryo Kajihara, Kei Amari, Yumiko Yokoyama, Maki Kaidoh, Mitsutoshi Sugano, Takayuki Honda, Yoshiko Kawai, and Toshio Ohhashi

Abstract

Background: Previous animal studies have shown that intragastric administration of water can accelerate mesenteric lymph flow. Similarly, human studies have shown that abdominal breathing can induce thoracic lymph drainage. In these studies, lymph flow was measured by hemodilution and a corresponding reduction in blood anti-diuretic hormone (ADH) levels, the latter being linked to urine osmolarity. Hence, we questioned if induction of lymph flow through water administration and supine positioning could be measured by monitoring urine osmolarity. Methods and Results: Volunteers were given 250 mL of distilled water and then made to rest for either 10 or 30 minutes in a supine position. Blood samples were taken pre and postrest to monitor changes in plasma ADH, total protein, plasma albumin, red blood cell, and hemoglobin concentrations. Urine was collected to monitor [Na+], [Cl-], and osmolarity. Intake of 250 mL distilled water with 10-minute rest caused a significant reduction in plasma ADH concentration, with decreases in urine [Na+], [Cl-], and osmolarity. We found a linear relationship between the ratio of plasma ADH concentrations after/before rest (between 1.1 and 3.0 pg$mL) and the ratio of urine osmolarity after/before rest (between 180 and 601 mOsm$L). Conclusions: Intake of 250 mL distilled water with 10-minute rest in a supine position caused hemodilution and a reduction in urine osmolarity consistent with thoracic lymph drainage. Urine osmolarity is a simple, safe clinical measure for monitoring lymph flow that could be used to evaluate the technique of lymph edema therapists. [ABSTRACT FROM AUTHOR]

Published
2020
Full Text
View/download PDF

15. Second Primary Squamous Cell Carcinoma Arising in a Skin Flap: A Case Report and Literature Review on Etiologic Factors and Treatment Strategy

Author

Hiroshi Kurita, Sachiho Nagashio, Atsushi Shinohara, and Reiko Tokita

Subjects
Male, medicine.medical_specialty, medicine.medical_treatment, Skin flap, Alphapapillomavirus, Surgical Flaps, Resection, Carcinoma, Humans, Medicine, Basal cell, Cyclin-Dependent Kinase Inhibitor p16, Aged, 80 and over, Bone Transplantation, Gingival Neoplasms, business.industry, Papillomavirus Infections, Neoplasms, Second Primary, Neck dissection, Forearm flap, Second primary cancer, medicine.disease, eye diseases, Neoplasm Proteins, Surgery, Tumor Virus Infections, stomatognathic diseases, Otorhinolaryngology, Erythroplasia, Carcinoma, Squamous Cell, Neck Dissection, Treatment strategy, Mandibular Reconstruction, Oral Surgery, business
Abstract

The number of reports describing malignant tumors arising in flaps has been increasing recently. This report describes the case of a patient who had a second squamous cell carcinoma (SCC) in the center of the skin island of a forearm flap raised approximately 6 years previously. Histopathologic examination suggested human papillomavirus infection and "mucosalization" of the flap. In addition, the authors review previous cases, with special attention to etiologic factors and surgical strategies. There might be a causal relation between second primary SCC in the flap and the carcinogenic effect (chronic inflammation) induced by the environment surrounding the squamous epithelium of the flap. If environmental factors have greatly contributed to the occurrence of a tumor, there is a potential for tumor occurrence in the remaining flap. There also might be a potential for generating second primary SCC in these foci. The results suggested that total resection of the flap might be recommended in the surgical resection of second primary SCC arising in a flap.

Published
2013
Full Text
View/download PDF

16. Water intake increases mesenteric lymph flow and the total flux of albumin, long-chain fatty acids, and IL-22 in rats: new concept of absorption in jejunum.

Author

Sachiho Nagashio, Kumiko Ajima, Daisuke Maejima, Hideki Sanjo, Ryo Kajihara, Moyuru Hayashi, Tomomi Watanabe-Asaka, Maki Kaidoh, Yumiko Yokoyama, Shinshuke Taki, Yoshiko Kawai, and Toshio Ohhashi

Abstract

The traditional Japanese health care custom recommends that a suitable volume of water is consumed. However, physiological and immunological mechanisms in support of this practice are unknown. Therefore, we conducted rat and rabbit in vivo experiments to investigate the effects of intragastric administration of distilled water on the jejunal-originated lymph flow and the concentrations and total flux of cells, albumin, long-chain fatty acids, and innate lymphoid cell 3 (ILC-3)-secreted interleukin-22 (IL-22) through mesenteric lymph vessels. The distribution and activity of ILC-3 in rat small intestine by water intake were evaluated using flow cytometry and RT-PCR. The intragastric administration of distilled water caused significant increases in rat mesenteric lymph flow and in the total flux of cells, albumin, long-chain fatty acids, and IL-22 through the lymph vessels. Intravenously injected Evans blue dye was rapidly transported into rabbit mesenteric lymph vessel and cisterna chyli. The distribution of ILC-3 and the expression of IL-22 mRNA were maximal in the lamina propria cells of the rat jejunum. No significant presence of ILC-3 in the lymph was observed in the control and under water intake conditions. In conclusion, the absorbed water in the jejunum is transported through mesenteric lymph vessels. The higher permeability of albumin in the jejunal microcirculation may play key roles in the transport of consumed water and the reservoir and transporter of long-chain fatty acids. Water intake also accelerates the transfer of IL-22 to the mesenteric lymph, which may contribute, in part, to maintaining and promoting the innate immunity in the body. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results